id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-274424-juj71nc5	Pulford, David J.	Intracellular processing of the porcine coronavirus transmissible gastroenteritis virus spike protein expressed by recombinant vaccinia virus	1991-06-30		.txt	text/plain	4901	231	49	Abstract The Spike (S) protein from a virulent British field isolate of porcine transmissible gastroenteritis virus (TGEV) FS772/70 was constructed from cDNA and inserted into the vaccinia virus (VV) thymidine kinase gene locus under the control of the VV early/late gene P7.5K promoter. In this paper we report the construction of a FS772/ 70 cDNA S gene and its expression by a recombinant vaccina virus (rVV) to study the antigenicity and cellular localization of the S protein. Expression of the recombinant spike antigens TGEV gene products synthesized by vTS-1 and vTSA-1 were analyzed by pulse labeling rVV-infected HTK-cells with L-[35S]methionine in the presence or absence of 10 pg ml-' tunicamycin for 6 hr. The lack of detectable ~160 or ~130 in the culture medium of vTS-l-and vTSA-l-infected cells, respectively (Fig. 4 lanes 4 and 7) , could result from the levels being too low to be detected by the antibodies due to reduction in synthesis or the lack of glycosylation of the S protein in the presence of tunicamycin.	./cache/cord-274424-juj71nc5.txt	./txt/cord-274424-juj71nc5.txt