id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-279598-xzionafe	Chang, Chia-Yu	Identification of Neutralizing Monoclonal Antibodies Targeting Novel Conformational Epitopes of the Porcine Epidemic Diarrhoea Virus Spike Protein	2019-02-21		.txt	text/plain	5378	267	53	title: Identification of Neutralizing Monoclonal Antibodies Targeting Novel Conformational Epitopes of the Porcine Epidemic Diarrhoea Virus Spike Protein However, E10E-1-10, which targets a novel neutralizing epitope as shown with ICC staining, was also capable of binding to the full-length PEDV spike protein as well as truncated PEDV proteins, S 1-639 , S 1-575 , S 1-509 , S 1-501 , and S 1-485 with appropriate dilution effects. Similar to the results obtained by ICC staining, E10E-1-10 showed no binding ability www.nature.com/scientificreports www.nature.com/scientificreports/ toward PEDV S 1-435 by ELISA and therefore, an NmAb targeting a novel epitope of the new variant of PEDV specifically at a.a. 435-485 in the S1 region was confirmed. To further determine whether the binding of neutralizing epitopes require conformational integrity, immunodot blotting was performed by denaturing the various truncated PEDV spike proteins. Cell Attachment Domains of the Porcine Epidemic Diarrhea Virus Spike Protein Are Key Targets of Neutralizing Antibodies	./cache/cord-279598-xzionafe.txt	./txt/cord-279598-xzionafe.txt