id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-289124-6w2zvvj1	Mok, Lawrence	Proteomic analysis of Pteropus alecto kidney cells in response to the viral mimic, Poly I:C	2015-11-02		.txt	text/plain	5444	356	57	Here, we investigated the differential protein expression of immortalised Pteropus alecto kidney cells (PaKiT03) following transfection with the viral mimic, Poly I:C. Two complementary proteomic approaches, difference gel electrophoresis (DIGE) and isobaric tagging for relative and absolute quantitation (iTRAQ) were used to quantify changes in protein expression following Poly I:C stimulation at 4, 8 and 20 hr post treatment (hpt). Through iTRAQ analysis we show that Poly I:C up-regulates key glycolytic enzymes at 4 hpt within PaKiT03 cells. The analysis with DIGE of Poly I:C transfected PaKiT03 cells showed over 215 individual spots differentially regulated, however only 25 spots could be unambiguously identified by LC-MS/MS. We further assessed the response of three glycolytic enzymes, α-enolase (Eno1), phosphoglycerate mutase 1 (Pgam1) and triosephosphate isomerase 1 (Tpi1) to Poly I:C across two human (HEK293T and HeLa) and two bat cell lines (PaKiT03, PaLuT02) using immunodetection.	./cache/cord-289124-6w2zvvj1.txt	./txt/cord-289124-6w2zvvj1.txt