id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-294712-kvvxmvqo	Pelosse, Martin	MultiBac: from protein complex structures to synthetic viral nanosystems	2017-10-30		.txt	text/plain	5379	272	38	The MultiBac baculovirus/insect cell expression vector system was conceived as a user-friendly, modular tool-kit for producing multiprotein complexes for structural biology applications. We then turned our attention to eukaryotic methods, specifically an expression vector system relying on a recombinant baculovirus, to infect insect cell cultures for producing the complexes in which we had interest. This particular feature, and the-at least conceptually-relative ease of manipulating baculovirus in the laboratory by non-expert users, was exciting and led us on to the development of MultiBac: a baculovirus/insect cell system specifically engineered for expressing functional multiprotein complexes in the quality and quantity required for high-resolution structural and mechanistic studies [7] [8] [9] [10] [11] . The introduced genome alterations resulted in a virus that exhibited delayed lysis of the insect cells, allowing the production of recombinant protein complexes at very high levels while the cells seemingly remained intact [11] . The MultiBac baculovirus/insect cell expression vector system for producing complex protein biologics	./cache/cord-294712-kvvxmvqo.txt	./txt/cord-294712-kvvxmvqo.txt