id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-303494-tofch4j7	Bai, Juan	Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine	2014-12-30		.txt	text/plain	3854	198	57	In order to map the minimal sequences of the epitopes recognized by McAbs, the series of truncated recombinant proteins were used in Western blot was to identify the reactivity of each of 10 anti-VP1 McAbs. The results showed that McAbs (6E11, 7A7 and 7C9) could reacted with fragments (F11, F9 and F3) containing the six amino acids V(2)ENAEK(7), but not reacted with the fragments with the deletion of any one of the six amino acids (F10, F7 and F8). Meanwhile, those purified truncated fragment proteins were used as the antigens in ELISA to detect the levels of those McAbs. The results showed that the OD 450 values of McAbs (6E11, 7A7 and 7C9) in F1, F3, F9 and F11 groups were significantly higher than those in F6, F7, F8 and F10 (p < 0.01) ( Figure 4A ).	./cache/cord-303494-tofch4j7.txt	./txt/cord-303494-tofch4j7.txt