id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-319517-denczc6t	Salipalli, Sandeep	Recent advances in live cell imaging of hepatoma cells	2014-07-08		.txt	text/plain	9184	433	46	This review aims to provide an overview of the recent developments in the field of marker proteins (bioluminescence and fluorescent) and methodologies (fluorescent resonance energy transfer, fluorescent recovery after photobleaching and proximity ligation assay) employed as to achieve an improved imaging of biological processes in hepatoma cells. The success of live cell imaging relies on various factors including the specific imaging system, climate controlling devices for cultured cells under investigation, construction of recombinant plasmid DNA, transfer and expression of candidate genes and/or fluorescent proteins in mammalian cells. T4SS (Bartonella sp.) 60-70% 50% >70% >60% 50-60% [76] [77] [78] lipofection (1 μg) methods were used for transfection of plasmid encoding fluorescent protein (pEGFP and pEYFP) tagged to human and mouse ADRP genes in Huh-7 cells as to study the pathways of lipid droplet metabolism.	./cache/cord-319517-denczc6t.txt	./txt/cord-319517-denczc6t.txt