id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-320790-ley91488	Fogg, M. J.	Application of the use of high‐throughput technologies to the determination of protein structures of bacterial and viral pathogens	2006-10-04		.txt	text/plain	7066	320	47	Here the impact of several technologies on the structural determination of proteins from human pathogens is illustrated with selected examples, including the parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens. Here the impact of several technologies on the structural determination of proteins from human pathogens is illustrated with selected examples, including the parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens. Further development and uptake of these methodologies (see Aricescu, Assenberg et al., 2006; Aricescu, Lu et al., 2006) is likely to have a major impact on the study of difficult viral targets, as indicated by preliminary results for EBV proteins, where insect-cell expression yielded soluble protein for 50% of the proteins tested (Tarbouriech et al., 2006) Here we illustrate the impact of a number of particular SPINE-based technologies on the structure determination of pathogen targets, namely (i) multiple-construct design for a single target, (ii) optimization of protein solubility, (iii) eukaryotic expression systems, (iv) standardized refolding protocols, (v) surface engineering and (vi) other biophysical characterization.	./cache/cord-320790-ley91488.txt	./txt/cord-320790-ley91488.txt