id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-327934-hjimlb6i	Acar, Delphine D.	Identification of peptide domains involved in the subcellular localization of the feline coronavirus 3b protein	2019-10-01		.txt	text/plain	6784	352	44	Transfection of different cell types with FCoV 3b fused to enhanced green fluorescent protein (EGFP) or 3×FLAG confirmed localization of FCoV 3b in the mitochondria and nucleoli. Transport of larger proteins to the nucleus, however, is an ATP-dependent active process driven by nuclear localization signals (NLSs), which are typically characterized by clusters of basic amino acids (reviewed in [38] [39] [40] [41] [42] ). The nucleocapsid proteins of several members of the order Nidovirales, such as porcine reproductive and respiratory syndrome virus (PRRSV, family Arteriviridae) [70, [77] [78] [79] [80] , transmissible gastroenteritis virus (TGEV) [81] , mouse hepatitis virus (MHV) [81, 82] and infectious bronchitis virus (IBV) [82] [83] [84] [85] (family Coronaviridae), also target the nucleolus and have been shown to incorporate one or more potential NLSs. Similarly, the SARS-CoV 3b protein was predicted to have two overlapping NLSs, a pat4 and bipartite motif, that were shown to be associated with nuclear (nucleolar) localization [27] .	./cache/cord-327934-hjimlb6i.txt	./txt/cord-327934-hjimlb6i.txt