id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-342118-fsmuqktd	Dyakov, Ilya N.	FN3 protein fragment containing two type III fibronectin domains from B. longum GT15 binds to human tumor necrosis factor alpha in vitro	2020-08-06		.txt	text/plain	2445	150	57	Previously, based on the analysis of the genomes of 34 species of bifidobacteria, we 233 identified and characterized a species-specific cluster of PFNA genes, which consists of To develop a sandwich ELISA assay that would allow us to check the ability of the 298 recombinant FN3 protein to bind human cytokines, we raised polyclonal antibodies 299 specific to the recombinant FN3 protein. The resulting affinity 302 purified and concentrated preparation contained 1 mg of FN3-specific rabbit polyclonal 303 antibodies per 1 mL and was highly active -specific interaction with the FN3 protein 304 was detected even after 1:820000 dilution. To exclude the possibility of blockage of the cytokine-binding motif of the FN3 335 protein and its nonspecific adsorption onto the solid surface, we employed ELISA 336 scheme 3 described in the materials and methods section: The FN3-specific rabbit 337 polyclonal antibodies were first adsorbed onto the solid surface after which the FN3 338 protein was added as a "second layer".	./cache/cord-342118-fsmuqktd.txt	./txt/cord-342118-fsmuqktd.txt