id	sid	tid	token	lemma	pos
cord-104282-90t1m430	1	1	key	key	JJ
cord-104282-90t1m430	1	2	:	:	:
cord-104282-90t1m430	1	3	cord-104282	cord-104282	NNP
cord-104282-90t1m430	1	4	-	-	HYPH
cord-104282-90t1m430	1	5	90t1m430	90t1m430	CD
cord-104282-90t1m430	1	6	authors	author	NNS
cord-104282-90t1m430	1	7	:	:	:
cord-104282-90t1m430	2	1	nan	nan	NNP
cord-104282-90t1m430	2	2	title	title	NN
cord-104282-90t1m430	2	3	:	:	:
cord-104282-90t1m430	3	1	Microsomal	microsomal	JJ
cord-104282-90t1m430	3	2	aldehyde	aldehyde	NN
cord-104282-90t1m430	3	3	dehydrogenase	dehydrogenase	NN
cord-104282-90t1m430	3	4	is	be	VBZ
cord-104282-90t1m430	3	5	localized	localize	VBN
cord-104282-90t1m430	3	6	to	to	IN
cord-104282-90t1m430	3	7	the	the	DT
cord-104282-90t1m430	3	8	endoplasmic	endoplasmic	NN
cord-104282-90t1m430	3	9	reticulum	reticulum	NN
cord-104282-90t1m430	3	10	via	via	IN
cord-104282-90t1m430	3	11	its	-PRON-	PRP$
cord-104282-90t1m430	3	12	carboxyl	carboxyl	NN
cord-104282-90t1m430	3	13	-	-	HYPH
cord-104282-90t1m430	3	14	terminal	terminal	NN
cord-104282-90t1m430	3	15	35	35	CD
cord-104282-90t1m430	3	16	amino	amino	JJ
cord-104282-90t1m430	3	17	acids	acid	NNS
cord-104282-90t1m430	3	18	date	date	NN
cord-104282-90t1m430	3	19	:	:	:
cord-104282-90t1m430	3	20	1994	1994	CD
cord-104282-90t1m430	3	21	-	-	SYM
cord-104282-90t1m430	3	22	09	09	CD
cord-104282-90t1m430	3	23	-	-	HYPH
cord-104282-90t1m430	3	24	02	02	CD
cord-104282-90t1m430	3	25	journal	journal	NN
cord-104282-90t1m430	3	26	:	:	:
cord-104282-90t1m430	3	27	J	J	NNP
cord-104282-90t1m430	3	28	Cell	Cell	NNP
cord-104282-90t1m430	3	29	Biol	Biol	NNP
cord-104282-90t1m430	3	30	DOI	DOI	NNP
cord-104282-90t1m430	3	31	:	:	:
cord-104282-90t1m430	3	32	nan	nan	NNP
cord-104282-90t1m430	3	33	sha	sha	NNP
cord-104282-90t1m430	3	34	:	:	:
cord-104282-90t1m430	3	35	3c6ed103b79757989428ac0f837b7a69f0d62b86	3c6ed103b79757989428ac0f837b7a69f0d62b86	CD
cord-104282-90t1m430	3	36	doc_id	doc_id	CD
cord-104282-90t1m430	3	37	:	:	:
cord-104282-90t1m430	3	38	104282	104282	CD
cord-104282-90t1m430	3	39	cord_uid	cord_uid	NNS
cord-104282-90t1m430	3	40	:	:	:
cord-104282-90t1m430	4	1	90t1m430	90t1m430	NFP
cord-104282-90t1m430	4	2	Rat	Rat	NNP
cord-104282-90t1m430	4	3	microsomal	microsomal	NN
cord-104282-90t1m430	4	4	aldehyde	aldehyde	NN
cord-104282-90t1m430	4	5	dehydrogenase	dehydrogenase	NN
cord-104282-90t1m430	4	6	(	(	-LRB-
cord-104282-90t1m430	4	7	msALDH	msALDH	NNP
cord-104282-90t1m430	4	8	)	)	-RRB-
cord-104282-90t1m430	4	9	has	have	VBZ
cord-104282-90t1m430	4	10	no	no	DT
cord-104282-90t1m430	4	11	amino	amino	NN
cord-104282-90t1m430	4	12	-	-	HYPH
cord-104282-90t1m430	4	13	terminal	terminal	NN
cord-104282-90t1m430	4	14	signal	signal	NN
cord-104282-90t1m430	4	15	sequence	sequence	NN
cord-104282-90t1m430	4	16	,	,	,
cord-104282-90t1m430	4	17	but	but	CC
cord-104282-90t1m430	4	18	instead	instead	RB
cord-104282-90t1m430	4	19	it	-PRON-	PRP
cord-104282-90t1m430	4	20	has	have	VBZ
cord-104282-90t1m430	4	21	a	a	DT
cord-104282-90t1m430	4	22	characteristic	characteristic	JJ
cord-104282-90t1m430	4	23	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	4	24	domain	domain	NN
cord-104282-90t1m430	4	25	at	at	IN
cord-104282-90t1m430	4	26	the	the	DT
cord-104282-90t1m430	4	27	carboxyl	carboxyl	NN
cord-104282-90t1m430	4	28	terminus	terminus	NN
cord-104282-90t1m430	4	29	(	(	-LRB-
cord-104282-90t1m430	4	30	Miyauchi	Miyauchi	NNP
cord-104282-90t1m430	4	31	,	,	,
cord-104282-90t1m430	4	32	K.	K.	NNP
cord-104282-90t1m430	4	33	,	,	,
cord-104282-90t1m430	4	34	R.	R.	NNP
cord-104282-90t1m430	4	35	Masaki	Masaki	NNP
cord-104282-90t1m430	4	36	,	,	,
cord-104282-90t1m430	4	37	S.	S.	NNP
cord-104282-90t1m430	4	38	Taketani	Taketani	NNP
cord-104282-90t1m430	4	39	,	,	,
cord-104282-90t1m430	4	40	A.	A.	NNP
cord-104282-90t1m430	4	41	Yamamoto	Yamamoto	NNP
cord-104282-90t1m430	4	42	,	,	,
cord-104282-90t1m430	4	43	A.	A.	NNP
cord-104282-90t1m430	4	44	Akayama	Akayama	NNP
cord-104282-90t1m430	4	45	,	,	,
cord-104282-90t1m430	4	46	and	and	CC
cord-104282-90t1m430	4	47	Y.	Y.	NNP
cord-104282-90t1m430	4	48	Tashiro	Tashiro	NNP
cord-104282-90t1m430	4	49	.	.	.
cord-104282-90t1m430	5	1	1991	1991	CD
cord-104282-90t1m430	5	2	.	.	.
cord-104282-90t1m430	6	1	J.	J.	NNP
cord-104282-90t1m430	6	2	Biol	Biol	NNP
cord-104282-90t1m430	6	3	.	.	.
cord-104282-90t1m430	7	1	Chem	Chem	NNP
cord-104282-90t1m430	7	2	.	.	.
cord-104282-90t1m430	8	1	266:19536-	266:19536-	CD
cord-104282-90t1m430	8	2	19542	19542	CD
cord-104282-90t1m430	8	3	)	)	-RRB-
cord-104282-90t1m430	8	4	.	.	.
cord-104282-90t1m430	9	1	This	this	DT
cord-104282-90t1m430	9	2	membrane	membrane	NN
cord-104282-90t1m430	9	3	-	-	HYPH
cord-104282-90t1m430	9	4	bound	bind	VBN
cord-104282-90t1m430	9	5	enzyme	enzyme	NN
cord-104282-90t1m430	9	6	is	be	VBZ
cord-104282-90t1m430	9	7	a	a	DT
cord-104282-90t1m430	9	8	useful	useful	JJ
cord-104282-90t1m430	9	9	model	model	NN
cord-104282-90t1m430	9	10	protein	protein	NN
cord-104282-90t1m430	9	11	for	for	IN
cord-104282-90t1m430	9	12	studying	study	VBG
cord-104282-90t1m430	9	13	posttranslational	posttranslational	JJ
cord-104282-90t1m430	9	14	localization	localization	NN
cord-104282-90t1m430	9	15	to	to	IN
cord-104282-90t1m430	9	16	its	-PRON-	PRP$
cord-104282-90t1m430	9	17	final	final	JJ
cord-104282-90t1m430	9	18	destination	destination	NN
cord-104282-90t1m430	9	19	.	.	.
cord-104282-90t1m430	10	1	When	when	WRB
cord-104282-90t1m430	10	2	expressed	express	VBN
cord-104282-90t1m430	10	3	from	from	IN
cord-104282-90t1m430	10	4	cDNA	cdna	NN
cord-104282-90t1m430	10	5	in	in	IN
cord-104282-90t1m430	10	6	COS-1	COS-1	NNP
cord-104282-90t1m430	10	7	cells	cell	NNS
cord-104282-90t1m430	10	8	,	,	,
cord-104282-90t1m430	10	9	wild	wild	JJ
cord-104282-90t1m430	10	10	-	-	HYPH
cord-104282-90t1m430	10	11	type	type	NN
cord-104282-90t1m430	10	12	msALDH	msaldh	NN
cord-104282-90t1m430	10	13	is	be	VBZ
cord-104282-90t1m430	10	14	localized	localize	VBN
cord-104282-90t1m430	10	15	exclusively	exclusively	RB
cord-104282-90t1m430	10	16	in	in	IN
cord-104282-90t1m430	10	17	the	the	DT
cord-104282-90t1m430	10	18	well	well	RB
cord-104282-90t1m430	10	19	-	-	HYPH
cord-104282-90t1m430	10	20	developed	develop	VBN
cord-104282-90t1m430	10	21	ER	ER	NNP
cord-104282-90t1m430	10	22	.	.	.
cord-104282-90t1m430	11	1	The	the	DT
cord-104282-90t1m430	11	2	removal	removal	NN
cord-104282-90t1m430	11	3	of	of	IN
cord-104282-90t1m430	11	4	the	the	DT
cord-104282-90t1m430	11	5	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	11	6	domain	domain	NN
cord-104282-90t1m430	11	7	results	result	NNS
cord-104282-90t1m430	11	8	in	in	IN
cord-104282-90t1m430	11	9	the	the	DT
cord-104282-90t1m430	11	10	cytosolic	cytosolic	JJ
cord-104282-90t1m430	11	11	localization	localization	NN
cord-104282-90t1m430	11	12	of	of	IN
cord-104282-90t1m430	11	13	truncated	truncated	JJ
cord-104282-90t1m430	11	14	proteins	protein	NNS
cord-104282-90t1m430	11	15	,	,	,
cord-104282-90t1m430	11	16	thus	thus	RB
cord-104282-90t1m430	11	17	suggesting	suggest	VBG
cord-104282-90t1m430	11	18	that	that	IN
cord-104282-90t1m430	11	19	the	the	DT
cord-104282-90t1m430	11	20	portion	portion	NN
cord-104282-90t1m430	11	21	is	be	VBZ
cord-104282-90t1m430	11	22	responsible	responsible	JJ
cord-104282-90t1m430	11	23	for	for	IN
cord-104282-90t1m430	11	24	membrane	membrane	NN
cord-104282-90t1m430	11	25	anchoring	anchoring	NN
cord-104282-90t1m430	11	26	.	.	.
cord-104282-90t1m430	12	1	The	the	DT
cord-104282-90t1m430	12	2	last	last	JJ
cord-104282-90t1m430	12	3	35	35	CD
cord-104282-90t1m430	12	4	amino	amino	NN
cord-104282-90t1m430	12	5	acids	acid	NNS
cord-104282-90t1m430	12	6	of	of	IN
cord-104282-90t1m430	12	7	msALDH	msALDH	NNP
cord-104282-90t1m430	12	8	,	,	,
cord-104282-90t1m430	12	9	including	include	VBG
cord-104282-90t1m430	12	10	the	the	DT
cord-104282-90t1m430	12	11	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	12	12	domain	domain	NN
cord-104282-90t1m430	12	13	,	,	,
cord-104282-90t1m430	12	14	are	be	VBP
cord-104282-90t1m430	12	15	sufficient	sufficient	JJ
cord-104282-90t1m430	12	16	for	for	IN
cord-104282-90t1m430	12	17	targeting	target	VBG
cord-104282-90t1m430	12	18	of	of	IN
cord-104282-90t1m430	12	19	E.	E.	NNP
cord-104282-90t1m430	12	20	coli	coli	NNS
cord-104282-90t1m430	12	21	beta	beta	NN
cord-104282-90t1m430	12	22	-	-	HYPH
cord-104282-90t1m430	12	23	galactosidase	galactosidase	NN
cord-104282-90t1m430	12	24	to	to	IN
cord-104282-90t1m430	12	25	the	the	DT
cord-104282-90t1m430	12	26	ER	ER	NNP
cord-104282-90t1m430	12	27	membrane	membrane	NN
cord-104282-90t1m430	12	28	.	.	.
cord-104282-90t1m430	13	1	Further	further	JJ
cord-104282-90t1m430	13	2	studies	study	NNS
cord-104282-90t1m430	13	3	using	use	VBG
cord-104282-90t1m430	13	4	chloramphenicol	chloramphenicol	NNP
cord-104282-90t1m430	13	5	acetyltransferase	acetyltransferase	NNP
cord-104282-90t1m430	13	6	fusion	fusion	NN
cord-104282-90t1m430	13	7	proteins	protein	NNS
cord-104282-90t1m430	13	8	suggest	suggest	VBP
cord-104282-90t1m430	13	9	that	that	IN
cord-104282-90t1m430	13	10	two	two	CD
cord-104282-90t1m430	13	11	hydrophilic	hydrophilic	JJ
cord-104282-90t1m430	13	12	sequences	sequence	NNS
cord-104282-90t1m430	13	13	on	on	IN
cord-104282-90t1m430	13	14	either	either	DT
cord-104282-90t1m430	13	15	side	side	NN
cord-104282-90t1m430	13	16	of	of	IN
cord-104282-90t1m430	13	17	the	the	DT
cord-104282-90t1m430	13	18	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	13	19	domain	domain	NN
cord-104282-90t1m430	13	20	play	play	VBP
cord-104282-90t1m430	13	21	an	an	DT
cord-104282-90t1m430	13	22	important	important	JJ
cord-104282-90t1m430	13	23	role	role	NN
cord-104282-90t1m430	13	24	in	in	IN
cord-104282-90t1m430	13	25	ER	ER	NNP
cord-104282-90t1m430	13	26	targeting	targeting	NN
cord-104282-90t1m430	13	27	.	.	.
cord-104282-90t1m430	14	1	domain	domain	NN
cord-104282-90t1m430	14	2	results	result	VBZ
cord-104282-90t1m430	14	3	in	in	IN
cord-104282-90t1m430	14	4	the	the	DT
cord-104282-90t1m430	14	5	cytosolic	cytosolic	JJ
cord-104282-90t1m430	14	6	localization	localization	NN
cord-104282-90t1m430	14	7	of	of	IN
cord-104282-90t1m430	14	8	truncated	truncated	JJ
cord-104282-90t1m430	14	9	proteins	protein	NNS
cord-104282-90t1m430	14	10	,	,	,
cord-104282-90t1m430	14	11	thus	thus	RB
cord-104282-90t1m430	14	12	suggesting	suggest	VBG
cord-104282-90t1m430	14	13	that	that	IN
cord-104282-90t1m430	14	14	the	the	DT
cord-104282-90t1m430	14	15	portion	portion	NN
cord-104282-90t1m430	14	16	is	be	VBZ
cord-104282-90t1m430	14	17	responsible	responsible	JJ
cord-104282-90t1m430	14	18	for	for	IN
cord-104282-90t1m430	14	19	membrane	membrane	NN
cord-104282-90t1m430	14	20	anchoring	anchoring	NN
cord-104282-90t1m430	14	21	.	.	.
cord-104282-90t1m430	15	1	The	the	DT
cord-104282-90t1m430	15	2	last	last	JJ
cord-104282-90t1m430	15	3	35	35	CD
cord-104282-90t1m430	15	4	amino	amino	NN
cord-104282-90t1m430	15	5	acids	acid	NNS
cord-104282-90t1m430	15	6	of	of	IN
cord-104282-90t1m430	15	7	msALDH	msALDH	NNP
cord-104282-90t1m430	15	8	,	,	,
cord-104282-90t1m430	15	9	including	include	VBG
cord-104282-90t1m430	15	10	the	the	DT
cord-104282-90t1m430	15	11	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	15	12	domain	domain	NN
cord-104282-90t1m430	15	13	,	,	,
cord-104282-90t1m430	15	14	are	be	VBP
cord-104282-90t1m430	15	15	sufficient	sufficient	JJ
cord-104282-90t1m430	15	16	for	for	IN
cord-104282-90t1m430	15	17	targeting	target	VBG
cord-104282-90t1m430	15	18	of	of	IN
cord-104282-90t1m430	15	19	E.	E.	NNP
cord-104282-90t1m430	15	20	coli	coli	NNS
cord-104282-90t1m430	16	1	~5-galactosidase	~5-galactosidase	NNP
cord-104282-90t1m430	17	1	to	to	IN
cord-104282-90t1m430	17	2	the	the	DT
cord-104282-90t1m430	17	3	ER	ER	NNP
cord-104282-90t1m430	17	4	membrane	membrane	NN
cord-104282-90t1m430	17	5	.	.	.
cord-104282-90t1m430	18	1	Further	further	JJ
cord-104282-90t1m430	18	2	studies	study	NNS
cord-104282-90t1m430	18	3	using	use	VBG
cord-104282-90t1m430	18	4	chloramphenicol	chloramphenicol	NNP
cord-104282-90t1m430	18	5	acetyltransferase	acetyltransferase	NNP
cord-104282-90t1m430	18	6	fusion	fusion	NN
cord-104282-90t1m430	18	7	proteins	protein	NNS
cord-104282-90t1m430	18	8	suggest	suggest	VBP
cord-104282-90t1m430	18	9	that	that	IN
cord-104282-90t1m430	18	10	two	two	CD
cord-104282-90t1m430	18	11	hydrophilie	hydrophilie	NNP
cord-104282-90t1m430	18	12	sequences	sequence	NNS
cord-104282-90t1m430	18	13	on	on	IN
cord-104282-90t1m430	18	14	either	either	DT
cord-104282-90t1m430	18	15	side	side	NN
cord-104282-90t1m430	18	16	of	of	IN
cord-104282-90t1m430	18	17	the	the	DT
cord-104282-90t1m430	18	18	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	18	19	domain	domain	NN
cord-104282-90t1m430	18	20	play	play	VBP
cord-104282-90t1m430	18	21	an	an	DT
cord-104282-90t1m430	18	22	important	important	JJ
cord-104282-90t1m430	18	23	role	role	NN
cord-104282-90t1m430	18	24	in	in	IN
cord-104282-90t1m430	18	25	ER	ER	NNP
cord-104282-90t1m430	18	26	targeting	targeting	NN
cord-104282-90t1m430	18	27	.	.	.
cord-104282-90t1m430	19	1	great	great	JJ
cord-104282-90t1m430	19	2	deal	deal	NN
cord-104282-90t1m430	19	3	of	of	IN
cord-104282-90t1m430	19	4	attention	attention	NN
cord-104282-90t1m430	19	5	has	have	VBZ
cord-104282-90t1m430	19	6	been	be	VBN
cord-104282-90t1m430	19	7	paid	pay	VBN
cord-104282-90t1m430	19	8	to	to	TO
cord-104282-90t1m430	19	9	resolve	resolve	VB
cord-104282-90t1m430	19	10	the	the	DT
cord-104282-90t1m430	19	11	mechanism	mechanism	NN
cord-104282-90t1m430	19	12	for	for	IN
cord-104282-90t1m430	19	13	sorting	sort	VBG
cord-104282-90t1m430	19	14	and	and	CC
cord-104282-90t1m430	19	15	targeting	targeting	NN
cord-104282-90t1m430	19	16	of	of	IN
cord-104282-90t1m430	19	17	newly	newly	RB
cord-104282-90t1m430	19	18	synthesized	synthesize	VBN
cord-104282-90t1m430	19	19	proteins	protein	NNS
cord-104282-90t1m430	19	20	to	to	IN
cord-104282-90t1m430	19	21	their	-PRON-	PRP$
cord-104282-90t1m430	19	22	final	final	JJ
cord-104282-90t1m430	19	23	destinations	destination	NNS
cord-104282-90t1m430	19	24	,	,	,
cord-104282-90t1m430	19	25	and	and	CC
cord-104282-90t1m430	19	26	this	this	DT
cord-104282-90t1m430	19	27	is	be	VBZ
cord-104282-90t1m430	19	28	one	one	CD
cord-104282-90t1m430	19	29	of	of	IN
cord-104282-90t1m430	19	30	the	the	DT
cord-104282-90t1m430	19	31	fundamental	fundamental	JJ
cord-104282-90t1m430	19	32	problemsin	problemsin	JJ
cord-104282-90t1m430	19	33	cell	cell	NN
cord-104282-90t1m430	19	34	biology	biology	NN
cord-104282-90t1m430	19	35	.	.	.
cord-104282-90t1m430	20	1	Newly	newly	RB
cord-104282-90t1m430	20	2	synthesized	synthesize	VBN
cord-104282-90t1m430	20	3	proteins	protein	NNS
cord-104282-90t1m430	20	4	are	be	VBP
cord-104282-90t1m430	20	5	destined	destine	VBN
cord-104282-90t1m430	20	6	to	to	TO
cord-104282-90t1m430	20	7	follow	follow	VB
cord-104282-90t1m430	20	8	two	two	CD
cord-104282-90t1m430	20	9	distinct	distinct	JJ
cord-104282-90t1m430	20	10	routes	route	NNS
cord-104282-90t1m430	20	11	,	,	,
cord-104282-90t1m430	20	12	depending	depend	VBG
cord-104282-90t1m430	20	13	on	on	IN
cord-104282-90t1m430	20	14	the	the	DT
cord-104282-90t1m430	20	15	presence	presence	NN
cord-104282-90t1m430	20	16	or	or	CC
cord-104282-90t1m430	20	17	absence	absence	NN
cord-104282-90t1m430	20	18	of	of	IN
cord-104282-90t1m430	20	19	a	a	DT
cord-104282-90t1m430	20	20	signal	signal	JJ
cord-104282-90t1m430	20	21	sequence	sequence	NN
cord-104282-90t1m430	20	22	at	at	IN
cord-104282-90t1m430	20	23	their	-PRON-	PRP$
cord-104282-90t1m430	20	24	amino	amino	NN
cord-104282-90t1m430	20	25	termini	terminus	NNS
cord-104282-90t1m430	20	26	(	(	-LRB-
cord-104282-90t1m430	20	27	37	37	CD
cord-104282-90t1m430	20	28	)	)	-RRB-
cord-104282-90t1m430	20	29	.	.	.
cord-104282-90t1m430	21	1	The	the	DT
cord-104282-90t1m430	21	2	signal	signal	NN
cord-104282-90t1m430	21	3	recognition	recognition	NN
cord-104282-90t1m430	21	4	particle	particle	NN
cord-104282-90t1m430	21	5	(	(	-LRB-
cord-104282-90t1m430	21	6	SRP	SRP	NNP
cord-104282-90t1m430	21	7	)	)	-RRB-
cord-104282-90t1m430	21	8	t	t	NN
cord-104282-90t1m430	21	9	in	in	IN
cord-104282-90t1m430	21	10	the	the	DT
cord-104282-90t1m430	21	11	cytosol	cytosol	NN
cord-104282-90t1m430	21	12	recognizes	recognize	VBZ
cord-104282-90t1m430	21	13	and	and	CC
cord-104282-90t1m430	21	14	binds	bind	VBZ
cord-104282-90t1m430	21	15	to	to	IN
cord-104282-90t1m430	21	16	a	a	DT
cord-104282-90t1m430	21	17	signal	signal	JJ
cord-104282-90t1m430	21	18	sequence	sequence	NN
cord-104282-90t1m430	21	19	of	of	IN
cord-104282-90t1m430	21	20	nascent	nascent	JJ
cord-104282-90t1m430	21	21	peptides	peptide	NNS
cord-104282-90t1m430	21	22	.	.	.
cord-104282-90t1m430	22	1	The	the	DT
cord-104282-90t1m430	22	2	resulting	result	VBG
cord-104282-90t1m430	22	3	SRP	SRP	NNP
cord-104282-90t1m430	22	4	-	-	HYPH
cord-104282-90t1m430	22	5	ribosome	ribosome	NN
cord-104282-90t1m430	22	6	-	-	HYPH
cord-104282-90t1m430	22	7	nascent	nascent	JJ
cord-104282-90t1m430	22	8	peptides	peptide	NNS
cord-104282-90t1m430	22	9	complexes	complex	NNS
cord-104282-90t1m430	22	10	are	be	VBP
cord-104282-90t1m430	22	11	targeted	target	VBN
cord-104282-90t1m430	22	12	to	to	IN
cord-104282-90t1m430	22	13	the	the	DT
cord-104282-90t1m430	22	14	ER	ER	NNP
cord-104282-90t1m430	22	15	membrane	membrane	NN
cord-104282-90t1m430	22	16	through	through	IN
cord-104282-90t1m430	22	17	their	-PRON-	PRP$
cord-104282-90t1m430	22	18	interactions	interaction	NNS
cord-104282-90t1m430	22	19	with	with	IN
cord-104282-90t1m430	22	20	the	the	DT
cord-104282-90t1m430	22	21	docking	docking	NN
cord-104282-90t1m430	22	22	protein	protein	NN
cord-104282-90t1m430	22	23	complex	complex	NN
cord-104282-90t1m430	22	24	(	(	-LRB-
cord-104282-90t1m430	22	25	35	35	CD
cord-104282-90t1m430	22	26	,	,	,
cord-104282-90t1m430	22	27	36	36	CD
cord-104282-90t1m430	22	28	)	)	-RRB-
cord-104282-90t1m430	22	29	.	.	.
cord-104282-90t1m430	23	1	After	after	IN
cord-104282-90t1m430	23	2	translocation	translocation	NN
cord-104282-90t1m430	23	3	across	across	IN
cord-104282-90t1m430	23	4	the	the	DT
cord-104282-90t1m430	23	5	ER	ER	NNP
cord-104282-90t1m430	23	6	membrane	membrane	NN
cord-104282-90t1m430	23	7	,	,	,
cord-104282-90t1m430	23	8	secretory	secretory	NN
cord-104282-90t1m430	23	9	and	and	CC
cord-104282-90t1m430	23	10	plasma	plasma	NN
cord-104282-90t1m430	23	11	membrane	membrane	NN
cord-104282-90t1m430	23	12	proteins	protein	NNS
cord-104282-90t1m430	23	13	follow	follow	VBP
cord-104282-90t1m430	23	14	a	a	DT
cord-104282-90t1m430	23	15	common	common	JJ
cord-104282-90t1m430	23	16	pathway	pathway	NN
cord-104282-90t1m430	23	17	from	from	IN
cord-104282-90t1m430	23	18	the	the	DT
cord-104282-90t1m430	23	19	ER	ER	NNP
cord-104282-90t1m430	23	20	,	,	,
cord-104282-90t1m430	23	21	through	through	IN
cord-104282-90t1m430	23	22	the	the	DT
cord-104282-90t1m430	23	23	Golgi	Golgi	NNP
cord-104282-90t1m430	23	24	complex	complex	NN
cord-104282-90t1m430	23	25	,	,	,
cord-104282-90t1m430	23	26	to	to	IN
cord-104282-90t1m430	23	27	the	the	DT
cord-104282-90t1m430	23	28	cell	cell	NN
cord-104282-90t1m430	23	29	surface	surface	NN
cord-104282-90t1m430	23	30	by	by	IN
cord-104282-90t1m430	23	31	default	default	NN
cord-104282-90t1m430	23	32	with	with	IN
cord-104282-90t1m430	23	33	bulk	bulk	JJ
cord-104282-90t1m430	23	34	flow	flow	NN
cord-104282-90t1m430	23	35	of	of	IN
cord-104282-90t1m430	23	36	lipids	lipid	NNS
cord-104282-90t1m430	23	37	(	(	-LRB-
cord-104282-90t1m430	23	38	33	33	CD
cord-104282-90t1m430	23	39	)	)	-RRB-
cord-104282-90t1m430	23	40	.	.	.
cord-104282-90t1m430	24	1	Resident	resident	JJ
cord-104282-90t1m430	24	2	proteins	protein	NNS
cord-104282-90t1m430	24	3	in	in	IN
cord-104282-90t1m430	24	4	the	the	DT
cord-104282-90t1m430	24	5	central	central	JJ
cord-104282-90t1m430	24	6	vacuolar	vacuolar	NNP
cord-104282-90t1m430	24	7	system	system	NN
cord-104282-90t1m430	24	8	are	be	VBP
cord-104282-90t1m430	24	9	localized	localize	VBN
cord-104282-90t1m430	24	10	and	and	CC
cord-104282-90t1m430	24	11	retained	retain	VBN
cord-104282-90t1m430	24	12	in	in	IN
cord-104282-90t1m430	24	13	their	-PRON-	PRP$
cord-104282-90t1m430	24	14	final	final	JJ
cord-104282-90t1m430	24	15	destinations	destination	NNS
cord-104282-90t1m430	24	16	with	with	IN
cord-104282-90t1m430	24	17	the	the	DT
cord-104282-90t1m430	24	18	aid	aid	NN
cord-104282-90t1m430	24	19	of	of	IN
cord-104282-90t1m430	24	20	either	either	DT
cord-104282-90t1m430	24	21	specific	specific	JJ
cord-104282-90t1m430	24	22	targeting	targeting	NN
cord-104282-90t1m430	24	23	(	(	-LRB-
cord-104282-90t1m430	24	24	18	18	CD
cord-104282-90t1m430	24	25	)	)	-RRB-
cord-104282-90t1m430	24	26	or	or	CC
cord-104282-90t1m430	24	27	retention	retention	NN
cord-104282-90t1m430	24	28	signals	signal	NNS
cord-104282-90t1m430	24	29	(	(	-LRB-
cord-104282-90t1m430	24	30	25	25	CD
cord-104282-90t1m430	24	31	)	)	-RRB-
cord-104282-90t1m430	24	32	(	(	-LRB-
cord-104282-90t1m430	24	33	26	26	CD
cord-104282-90t1m430	24	34	)	)	-RRB-
cord-104282-90t1m430	24	35	(	(	-LRB-
cord-104282-90t1m430	24	36	27	27	CD
cord-104282-90t1m430	24	37	)	)	-RRB-
cord-104282-90t1m430	24	38	44	44	CD
cord-104282-90t1m430	24	39	)	)	-RRB-
cord-104282-90t1m430	24	40	.	.	.
cord-104282-90t1m430	25	1	On	on	IN
cord-104282-90t1m430	25	2	the	the	DT
cord-104282-90t1m430	25	3	other	other	JJ
cord-104282-90t1m430	25	4	hand	hand	NN
cord-104282-90t1m430	25	5	,	,	,
cord-104282-90t1m430	25	6	proteins	protein	NNS
cord-104282-90t1m430	25	7	without	without	IN
cord-104282-90t1m430	25	8	a	a	DT
cord-104282-90t1m430	25	9	signal	signal	JJ
cord-104282-90t1m430	25	10	sequence	sequence	NN
cord-104282-90t1m430	25	11	at	at	IN
cord-104282-90t1m430	25	12	their	-PRON-	PRP$
cord-104282-90t1m430	25	13	amino	amino	NN
cord-104282-90t1m430	25	14	termini	terminus	NNS
cord-104282-90t1m430	25	15	are	be	VBP
cord-104282-90t1m430	25	16	synthesized	synthesize	VBN
cord-104282-90t1m430	25	17	on	on	IN
cord-104282-90t1m430	25	18	free	free	JJ
cord-104282-90t1m430	25	19	polysomes	polysome	NNS
cord-104282-90t1m430	25	20	and	and	CC
cord-104282-90t1m430	25	21	1	1	CD
cord-104282-90t1m430	25	22	.	.	.
cord-104282-90t1m430	26	1	Abbreviations	abbreviation	NNS
cord-104282-90t1m430	26	2	used	use	VBN
cord-104282-90t1m430	26	3	in	in	IN
cord-104282-90t1m430	26	4	this	this	DT
cord-104282-90t1m430	26	5	paper	paper	NN
cord-104282-90t1m430	26	6	:	:	:
cord-104282-90t1m430	26	7	CAT	cat	NN
cord-104282-90t1m430	26	8	,	,	,
cord-104282-90t1m430	26	9	chloramphenicol	chloramphenicol	NN
cord-104282-90t1m430	26	10	acetyltransferase	acetyltransferase	NN
cord-104282-90t1m430	26	11	;	;	:
cord-104282-90t1m430	26	12	FP2	FP2	NNP
cord-104282-90t1m430	26	13	,	,	,
cord-104282-90t1m430	26	14	NADPH	nadph	NN
cord-104282-90t1m430	26	15	-	-	HYPH
cord-104282-90t1m430	26	16	cytochrome	cytochrome	NN
cord-104282-90t1m430	26	17	P-450	p-450	NN
cord-104282-90t1m430	26	18	reductase	reductase	NN
cord-104282-90t1m430	26	19	;	;	:
cord-104282-90t1m430	26	20	msALDH	msALDH	NNP
cord-104282-90t1m430	26	21	,	,	,
cord-104282-90t1m430	26	22	microsomal	microsomal	NN
cord-104282-90t1m430	26	23	aldehyde	aldehyde	NN
cord-104282-90t1m430	26	24	dehydrogenase	dehydrogenase	NN
cord-104282-90t1m430	26	25	;	;	:
cord-104282-90t1m430	26	26	PBS(+	PBS(+	NNP
cord-104282-90t1m430	26	27	)	)	-RRB-
cord-104282-90t1m430	26	28	,	,	,
cord-104282-90t1m430	26	29	PBS	PBS	NNP
cord-104282-90t1m430	26	30	containing	contain	VBG
cord-104282-90t1m430	26	31	1	1	CD
cord-104282-90t1m430	26	32	mM	mm	CD
cord-104282-90t1m430	26	33	CaCI2	CaCI2	NNP
cord-104282-90t1m430	26	34	and	and	CC
cord-104282-90t1m430	26	35	0.5	0.5	CD
cord-104282-90t1m430	26	36	mM	mm	CD
cord-104282-90t1m430	26	37	MgCI2	MgCI2	NNP
cord-104282-90t1m430	26	38	;	;	:
cord-104282-90t1m430	26	39	PDI	PDI	NNP
cord-104282-90t1m430	26	40	,	,	,
cord-104282-90t1m430	26	41	protein	protein	NN
cord-104282-90t1m430	26	42	disulfide	disulfide	NN
cord-104282-90t1m430	26	43	isomerase	isomerase	NN
cord-104282-90t1m430	26	44	;	;	:
cord-104282-90t1m430	26	45	PTP	PTP	NNP
cord-104282-90t1m430	26	46	,	,	,
cord-104282-90t1m430	26	47	protein	protein	NN
cord-104282-90t1m430	26	48	tyrosine	tyrosine	NN
cord-104282-90t1m430	26	49	phosphatase	phosphatase	NN
cord-104282-90t1m430	26	50	;	;	:
cord-104282-90t1m430	26	51	STE	STE	NNP
cord-104282-90t1m430	26	52	,	,	,
cord-104282-90t1m430	26	53	sucrose	sucrose	NN
cord-104282-90t1m430	26	54	solution	solution	NN
cord-104282-90t1m430	26	55	containing	contain	VBG
cord-104282-90t1m430	26	56	10	10	CD
cord-104282-90t1m430	26	57	mM	mm	CD
cord-104282-90t1m430	26	58	Tris	Tris	NNP
cord-104282-90t1m430	26	59	-	-	HYPH
cord-104282-90t1m430	26	60	HCl	HCl	NNP
cord-104282-90t1m430	26	61	,	,	,
cord-104282-90t1m430	26	62	pH	pH	NNP
cord-104282-90t1m430	26	63	7.4	7.4	CD
cord-104282-90t1m430	26	64	,	,	,
cord-104282-90t1m430	26	65	1	1	CD
cord-104282-90t1m430	26	66	mM	mm	CD
cord-104282-90t1m430	26	67	EDTA	edta	NN
cord-104282-90t1m430	26	68	,	,	,
cord-104282-90t1m430	27	1	10	10	CD
cord-104282-90t1m430	27	2	t	t	NN
cord-104282-90t1m430	27	3	~	~	CD
cord-104282-90t1m430	27	4	g	g	NN
cord-104282-90t1m430	27	5	/	/	SYM
cord-104282-90t1m430	27	6	ml	ml	NNP
cord-104282-90t1m430	27	7	leupepdn	leupepdn	NNP
cord-104282-90t1m430	27	8	A	A	NNP
cord-104282-90t1m430	27	9	,	,	,
cord-104282-90t1m430	27	10	0.5	0.5	CD
cord-104282-90t1m430	27	11	mM	mm	CD
cord-104282-90t1m430	27	12	PMSF	PMSF	NNP
cord-104282-90t1m430	27	13	,	,	,
cord-104282-90t1m430	27	14	and	and	CC
cord-104282-90t1m430	27	15	10	10	CD
cord-104282-90t1m430	27	16	U	u	NN
cord-104282-90t1m430	27	17	/	/	SYM
cord-104282-90t1m430	27	18	ml	ml	NNP
cord-104282-90t1m430	27	19	Trasyol	Trasyol	NNP
cord-104282-90t1m430	27	20	;	;	:
cord-104282-90t1m430	27	21	SRP	SRP	NNP
cord-104282-90t1m430	27	22	,	,	,
cord-104282-90t1m430	27	23	signal	signal	NN
cord-104282-90t1m430	27	24	recognition	recognition	NN
cord-104282-90t1m430	27	25	particle	particle	NN
cord-104282-90t1m430	27	26	.	.	.
cord-104282-90t1m430	28	1	are	be	VBP
cord-104282-90t1m430	28	2	posttranslationally	posttranslationally	RB
cord-104282-90t1m430	28	3	directed	direct	VBN
cord-104282-90t1m430	28	4	to	to	IN
cord-104282-90t1m430	28	5	intracellular	intracellular	JJ
cord-104282-90t1m430	28	6	organdies	organdie	NNS
cord-104282-90t1m430	28	7	such	such	JJ
cord-104282-90t1m430	28	8	as	as	IN
cord-104282-90t1m430	28	9	mitochondria	mitochondrion	NNS
cord-104282-90t1m430	28	10	,	,	,
cord-104282-90t1m430	28	11	peroxisomes	peroxisome	NNS
cord-104282-90t1m430	28	12	,	,	,
cord-104282-90t1m430	28	13	the	the	DT
cord-104282-90t1m430	28	14	nucleus	nucleus	NN
cord-104282-90t1m430	28	15	,	,	,
cord-104282-90t1m430	28	16	and	and	CC
cord-104282-90t1m430	28	17	the	the	DT
cord-104282-90t1m430	28	18	ER	ER	NNP
cord-104282-90t1m430	28	19	,	,	,
cord-104282-90t1m430	28	20	or	or	CC
cord-104282-90t1m430	28	21	they	-PRON-	PRP
cord-104282-90t1m430	28	22	remain	remain	VBP
cord-104282-90t1m430	28	23	in	in	IN
cord-104282-90t1m430	28	24	the	the	DT
cord-104282-90t1m430	28	25	cytosol	cytosol	NN
cord-104282-90t1m430	28	26	.	.	.
cord-104282-90t1m430	29	1	Much	much	JJ
cord-104282-90t1m430	29	2	is	be	VBZ
cord-104282-90t1m430	29	3	known	know	VBN
cord-104282-90t1m430	29	4	regarding	regard	VBG
cord-104282-90t1m430	29	5	targeting	target	VBG
cord-104282-90t1m430	29	6	signals	signal	NNS
cord-104282-90t1m430	29	7	(	(	-LRB-
cord-104282-90t1m430	29	8	14	14	CD
cord-104282-90t1m430	29	9	,	,	,
cord-104282-90t1m430	29	10	16	16	CD
cord-104282-90t1m430	29	11	,	,	,
cord-104282-90t1m430	29	12	39	39	CD
cord-104282-90t1m430	29	13	)	)	-RRB-
cord-104282-90t1m430	29	14	and	and	CC
cord-104282-90t1m430	29	15	cytosolic	cytosolic	JJ
cord-104282-90t1m430	29	16	protein	protein	NN
cord-104282-90t1m430	29	17	factors	factor	NNS
cord-104282-90t1m430	29	18	(	(	-LRB-
cord-104282-90t1m430	29	19	1	1	CD
cord-104282-90t1m430	29	20	,	,	,
cord-104282-90t1m430	29	21	15	15	CD
cord-104282-90t1m430	29	22	)	)	-RRB-
cord-104282-90t1m430	29	23	by	by	IN
cord-104282-90t1m430	29	24	which	which	WDT
cord-104282-90t1m430	29	25	proteins	protein	NNS
cord-104282-90t1m430	29	26	are	be	VBP
cord-104282-90t1m430	29	27	imported	import	VBN
cord-104282-90t1m430	29	28	into	into	IN
cord-104282-90t1m430	29	29	these	these	DT
cord-104282-90t1m430	29	30	organelles	organelle	NNS
cord-104282-90t1m430	29	31	.	.	.
cord-104282-90t1m430	30	1	However	however	RB
cord-104282-90t1m430	30	2	,	,	,
cord-104282-90t1m430	30	3	less	less	JJR
cord-104282-90t1m430	30	4	is	be	VBZ
cord-104282-90t1m430	30	5	known	know	VBN
cord-104282-90t1m430	30	6	regarding	regard	VBG
cord-104282-90t1m430	30	7	posttranslational	posttranslational	JJ
cord-104282-90t1m430	30	8	targeting	targeting	NN
cord-104282-90t1m430	30	9	of	of	IN
cord-104282-90t1m430	30	10	integral	integral	JJ
cord-104282-90t1m430	30	11	membrane	membrane	NN
cord-104282-90t1m430	30	12	proteins	protein	NNS
cord-104282-90t1m430	30	13	to	to	IN
cord-104282-90t1m430	30	14	the	the	DT
cord-104282-90t1m430	30	15	outer	outer	JJ
cord-104282-90t1m430	30	16	mitochondrial	mitochondrial	NN
cord-104282-90t1m430	30	17	membrane	membrane	NN
cord-104282-90t1m430	30	18	,	,	,
cord-104282-90t1m430	30	19	the	the	DT
cord-104282-90t1m430	30	20	peroxisomal	peroxisomal	JJ
cord-104282-90t1m430	30	21	membrane	membrane	NN
cord-104282-90t1m430	30	22	,	,	,
cord-104282-90t1m430	30	23	or	or	CC
cord-104282-90t1m430	30	24	the	the	DT
cord-104282-90t1m430	30	25	ER	ER	NNP
cord-104282-90t1m430	30	26	membrane	membrane	NN
cord-104282-90t1m430	30	27	.	.	.
cord-104282-90t1m430	31	1	Cytochrome	cytochrome	NN
cord-104282-90t1m430	31	2	b5	b5	NNP
cord-104282-90t1m430	31	3	has	have	VBZ
cord-104282-90t1m430	31	4	a	a	DT
cord-104282-90t1m430	31	5	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	31	6	domain	domain	NN
cord-104282-90t1m430	31	7	instead	instead	RB
cord-104282-90t1m430	31	8	of	of	IN
cord-104282-90t1m430	31	9	an	an	DT
cord-104282-90t1m430	31	10	amino	amino	NN
cord-104282-90t1m430	31	11	-	-	HYPH
cord-104282-90t1m430	31	12	terminal	terminal	NN
cord-104282-90t1m430	31	13	signal	signal	NN
cord-104282-90t1m430	31	14	sequence	sequence	NN
cord-104282-90t1m430	31	15	at	at	IN
cord-104282-90t1m430	31	16	its	-PRON-	PRP$
cord-104282-90t1m430	31	17	carboxyl	carboxyl	NN
cord-104282-90t1m430	31	18	terminus	terminus	NN
cord-104282-90t1m430	31	19	(	(	-LRB-
cord-104282-90t1m430	31	20	42	42	CD
cord-104282-90t1m430	31	21	)	)	-RRB-
cord-104282-90t1m430	31	22	.	.	.
cord-104282-90t1m430	32	1	For	for	IN
cord-104282-90t1m430	32	2	a	a	DT
cord-104282-90t1m430	32	3	long	long	JJ
cord-104282-90t1m430	32	4	time	time	NN
cord-104282-90t1m430	32	5	,	,	,
cord-104282-90t1m430	32	6	cytochrome	cytochrome	NN
cord-104282-90t1m430	32	7	b5	b5	NNP
cord-104282-90t1m430	32	8	has	have	VBZ
cord-104282-90t1m430	32	9	been	be	VBN
cord-104282-90t1m430	32	10	noted	note	VBN
cord-104282-90t1m430	32	11	to	to	TO
cord-104282-90t1m430	32	12	be	be	VB
cord-104282-90t1m430	32	13	a	a	DT
cord-104282-90t1m430	32	14	typical	typical	JJ
cord-104282-90t1m430	32	15	membrane	membrane	NN
cord-104282-90t1m430	32	16	protein	protein	NN
cord-104282-90t1m430	32	17	inserted	insert	VBD
cord-104282-90t1m430	32	18	posttranslationally	posttranslationally	RB
cord-104282-90t1m430	32	19	into	into	IN
cord-104282-90t1m430	32	20	microsomal	microsomal	NNP
cord-104282-90t1m430	32	21	membranes	membrane	NNS
cord-104282-90t1m430	32	22	through	through	IN
cord-104282-90t1m430	32	23	the	the	DT
cord-104282-90t1m430	32	24	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	32	25	domain	domain	NN
cord-104282-90t1m430	32	26	.	.	.
cord-104282-90t1m430	33	1	Besides	besides	RB
cord-104282-90t1m430	33	2	,	,	,
cord-104282-90t1m430	33	3	it	-PRON-	PRP
cord-104282-90t1m430	33	4	has	have	VBZ
cord-104282-90t1m430	33	5	been	be	VBN
cord-104282-90t1m430	33	6	reported	report	VBN
cord-104282-90t1m430	33	7	that	that	IN
cord-104282-90t1m430	33	8	cytochrome	cytochrome	NN
cord-104282-90t1m430	33	9	b5	b5	NN
cord-104282-90t1m430	33	10	is	be	VBZ
cord-104282-90t1m430	33	11	localized	localize	VBN
cord-104282-90t1m430	33	12	in	in	IN
cord-104282-90t1m430	33	13	any	any	DT
cord-104282-90t1m430	33	14	membranes	membrane	NNS
cord-104282-90t1m430	33	15	of	of	IN
cord-104282-90t1m430	33	16	intracellular	intracellular	JJ
cord-104282-90t1m430	33	17	organdies	organdie	NNS
cord-104282-90t1m430	33	18	,	,	,
cord-104282-90t1m430	33	19	including	include	VBG
cord-104282-90t1m430	33	20	the	the	DT
cord-104282-90t1m430	33	21	ER	ER	NNP
cord-104282-90t1m430	33	22	membrane	membrane	NN
cord-104282-90t1m430	33	23	,	,	,
cord-104282-90t1m430	33	24	the	the	DT
cord-104282-90t1m430	33	25	Golgi	Golgi	NNP
cord-104282-90t1m430	33	26	membrane	membrane	NN
cord-104282-90t1m430	33	27	,	,	,
cord-104282-90t1m430	33	28	the	the	DT
cord-104282-90t1m430	33	29	plasma	plasma	NN
cord-104282-90t1m430	33	30	membrane	membrane	NN
cord-104282-90t1m430	33	31	,	,	,
cord-104282-90t1m430	33	32	and	and	CC
cord-104282-90t1m430	33	33	the	the	DT
cord-104282-90t1m430	33	34	outer	outer	JJ
cord-104282-90t1m430	33	35	mitochondrial	mitochondrial	NN
cord-104282-90t1m430	33	36	membrane	membrane	NN
cord-104282-90t1m430	33	37	(	(	-LRB-
cord-104282-90t1m430	33	38	47	47	CD
cord-104282-90t1m430	33	39	)	)	-RRB-
cord-104282-90t1m430	33	40	.	.	.
cord-104282-90t1m430	34	1	Experiments	experiment	NNS
cord-104282-90t1m430	34	2	using	use	VBG
cord-104282-90t1m430	34	3	SRP	SRP	NNP
cord-104282-90t1m430	34	4	-	-	HYPH
cord-104282-90t1m430	34	5	depleted	deplete	VBN
cord-104282-90t1m430	34	6	or	or	CC
cord-104282-90t1m430	34	7	-supplemented	-supplemented	JJ
cord-104282-90t1m430	35	1	in	in	IN
cord-104282-90t1m430	35	2	vitro	vitro	FW
cord-104282-90t1m430	35	3	systems	system	NNS
cord-104282-90t1m430	35	4	have	have	VBP
cord-104282-90t1m430	35	5	shown	show	VBN
cord-104282-90t1m430	35	6	that	that	IN
cord-104282-90t1m430	35	7	this	this	DT
cord-104282-90t1m430	35	8	protein	protein	NN
cord-104282-90t1m430	35	9	does	do	VBZ
cord-104282-90t1m430	35	10	not	not	RB
cord-104282-90t1m430	35	11	require	require	VB
cord-104282-90t1m430	35	12	SRP	SRP	NNP
cord-104282-90t1m430	35	13	for	for	IN
cord-104282-90t1m430	35	14	insertion	insertion	NN
cord-104282-90t1m430	35	15	into	into	IN
cord-104282-90t1m430	35	16	microsomal	microsomal	JJ
cord-104282-90t1m430	35	17	membranes	membrane	NNS
cord-104282-90t1m430	35	18	(	(	-LRB-
cord-104282-90t1m430	35	19	3	3	CD
cord-104282-90t1m430	35	20	)	)	-RRB-
cord-104282-90t1m430	35	21	.	.	.
cord-104282-90t1m430	36	1	Therefore	therefore	RB
cord-104282-90t1m430	36	2	,	,	,
cord-104282-90t1m430	36	3	the	the	DT
cord-104282-90t1m430	36	4	carboxylterminal	carboxylterminal	JJ
cord-104282-90t1m430	36	5	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	36	6	sequence	sequence	NN
cord-104282-90t1m430	36	7	was	be	VBD
cord-104282-90t1m430	36	8	termed	term	VBN
cord-104282-90t1m430	36	9	an	an	DT
cord-104282-90t1m430	36	10	"	"	``
cord-104282-90t1m430	36	11	insertion	insertion	NN
cord-104282-90t1m430	36	12	"	"	''
cord-104282-90t1m430	36	13	sequence	sequence	NN
cord-104282-90t1m430	36	14	through	through	IN
cord-104282-90t1m430	36	15	which	which	WDT
cord-104282-90t1m430	36	16	spontaneous	spontaneous	JJ
cord-104282-90t1m430	36	17	integration	integration	NN
cord-104282-90t1m430	36	18	of	of	IN
cord-104282-90t1m430	36	19	cytochrome	cytochrome	NN
cord-104282-90t1m430	36	20	b5	b5	NN
cord-104282-90t1m430	36	21	into	into	IN
cord-104282-90t1m430	36	22	any	any	DT
cord-104282-90t1m430	36	23	exposed	expose	VBN
cord-104282-90t1m430	36	24	membranes	membrane	NNS
cord-104282-90t1m430	36	25	could	could	MD
cord-104282-90t1m430	36	26	occur	occur	VB
cord-104282-90t1m430	36	27	not	not	RB
cord-104282-90t1m430	36	28	only	only	RB
cord-104282-90t1m430	36	29	in	in	IN
cord-104282-90t1m430	36	30	vitro	vitro	FW
cord-104282-90t1m430	36	31	,	,	,
cord-104282-90t1m430	36	32	but	but	CC
cord-104282-90t1m430	36	33	also	also	RB
cord-104282-90t1m430	36	34	in	in	IN
cord-104282-90t1m430	36	35	vivo	vivo	NNP
cord-104282-90t1m430	36	36	(	(	-LRB-
cord-104282-90t1m430	36	37	5	5	CD
cord-104282-90t1m430	36	38	)	)	-RRB-
cord-104282-90t1m430	36	39	.	.	.
cord-104282-90t1m430	37	1	However	however	RB
cord-104282-90t1m430	37	2	,	,	,
cord-104282-90t1m430	37	3	recent	recent	JJ
cord-104282-90t1m430	37	4	studies	study	NNS
cord-104282-90t1m430	37	5	have	have	VBP
cord-104282-90t1m430	37	6	shown	show	VBN
cord-104282-90t1m430	37	7	that	that	DT
cord-104282-90t1m430	37	8	cytochrome	cytochrome	NN
cord-104282-90t1m430	37	9	b5	b5	NN
cord-104282-90t1m430	37	10	is	be	VBZ
cord-104282-90t1m430	37	11	restricted	restrict	VBN
cord-104282-90t1m430	37	12	in	in	IN
cord-104282-90t1m430	37	13	the	the	DT
cord-104282-90t1m430	37	14	ER	ER	NNP
cord-104282-90t1m430	37	15	in	in	IN
cord-104282-90t1m430	37	16	vivo	vivo	NN
cord-104282-90t1m430	37	17	(	(	-LRB-
cord-104282-90t1m430	37	18	11	11	CD
cord-104282-90t1m430	37	19	)	)	-RRB-
cord-104282-90t1m430	37	20	,	,	,
cord-104282-90t1m430	37	21	and	and	CC
cord-104282-90t1m430	37	22	that	that	IN
cord-104282-90t1m430	37	23	the	the	DT
cord-104282-90t1m430	37	24	last	last	JJ
cord-104282-90t1m430	37	25	10	10	CD
cord-104282-90t1m430	37	26	amino	amino	NN
cord-104282-90t1m430	37	27	acids	acid	NNS
cord-104282-90t1m430	37	28	of	of	IN
cord-104282-90t1m430	37	29	this	this	DT
cord-104282-90t1m430	37	30	protein	protein	NN
cord-104282-90t1m430	37	31	adjacent	adjacent	JJ
cord-104282-90t1m430	37	32	to	to	IN
cord-104282-90t1m430	37	33	the	the	DT
cord-104282-90t1m430	37	34	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	37	35	domain	domain	NN
cord-104282-90t1m430	37	36	are	be	VBP
cord-104282-90t1m430	37	37	important	important	JJ
cord-104282-90t1m430	37	38	for	for	IN
cord-104282-90t1m430	37	39	its	-PRON-	PRP$
cord-104282-90t1m430	37	40	targeting	targeting	NN
cord-104282-90t1m430	37	41	to	to	IN
cord-104282-90t1m430	37	42	the	the	DT
cord-104282-90t1m430	37	43	ER	ER	NNP
cord-104282-90t1m430	37	44	(	(	-LRB-
cord-104282-90t1m430	37	45	23	23	CD
cord-104282-90t1m430	37	46	)	)	-RRB-
cord-104282-90t1m430	37	47	.	.	.
cord-104282-90t1m430	38	1	Recently	recently	RB
cord-104282-90t1m430	38	2	,	,	,
cord-104282-90t1m430	38	3	we	-PRON-	PRP
cord-104282-90t1m430	38	4	have	have	VBP
cord-104282-90t1m430	38	5	isolated	isolate	VBN
cord-104282-90t1m430	38	6	and	and	CC
cord-104282-90t1m430	38	7	sequenced	sequence	VBN
cord-104282-90t1m430	38	8	a	a	DT
cord-104282-90t1m430	38	9	full	full	JJ
cord-104282-90t1m430	38	10	-	-	HYPH
cord-104282-90t1m430	38	11	length	length	NN
cord-104282-90t1m430	38	12	cDNA	cdna	NN
cord-104282-90t1m430	38	13	for	for	IN
cord-104282-90t1m430	38	14	rat	rat	NNP
cord-104282-90t1m430	38	15	microsomal	microsomal	NNP
cord-104282-90t1m430	38	16	aldehyde	aldehyde	NN
cord-104282-90t1m430	38	17	dehydrogenase	dehydrogenase	NN
cord-104282-90t1m430	38	18	(	(	-LRB-
cord-104282-90t1m430	38	19	msALDH	msALDH	NNP
cord-104282-90t1m430	38	20	)	)	-RRB-
cord-104282-90t1m430	38	21	(	(	-LRB-
cord-104282-90t1m430	38	22	24	24	CD
cord-104282-90t1m430	38	23	)	)	-RRB-
cord-104282-90t1m430	38	24	.	.	.
cord-104282-90t1m430	39	1	The	the	DT
cord-104282-90t1m430	39	2	deduced	deduce	VBN
cord-104282-90t1m430	39	3	amino	amino	NN
cord-104282-90t1m430	39	4	acid	acid	NN
cord-104282-90t1m430	39	5	sequence	sequence	NN
cord-104282-90t1m430	39	6	of	of	IN
cord-104282-90t1m430	39	7	this	this	DT
cord-104282-90t1m430	39	8	enzyme	enzyme	NN
cord-104282-90t1m430	39	9	prediets	prediets	NNP
cord-104282-90t1m430	39	10	a	a	DT
cord-104282-90t1m430	39	11	similar	similar	JJ
cord-104282-90t1m430	39	12	molecular	molecular	JJ
cord-104282-90t1m430	39	13	structure	structure	NN
cord-104282-90t1m430	39	14	to	to	IN
cord-104282-90t1m430	39	15	that	that	DT
cord-104282-90t1m430	39	16	of	of	IN
cord-104282-90t1m430	39	17	cytochrome	cytochrome	NN
cord-104282-90t1m430	39	18	b5	b5	NN
cord-104282-90t1m430	39	19	,	,	,
cord-104282-90t1m430	39	20	a	a	DT
cord-104282-90t1m430	39	21	bulky	bulky	JJ
cord-104282-90t1m430	39	22	amino	amino	NN
cord-104282-90t1m430	39	23	-	-	HYPH
cord-104282-90t1m430	39	24	terminal	terminal	NN
cord-104282-90t1m430	39	25	domain	domain	NN
cord-104282-90t1m430	39	26	without	without	IN
cord-104282-90t1m430	39	27	an	an	DT
cord-104282-90t1m430	39	28	amino	amino	NN
cord-104282-90t1m430	39	29	-	-	HYPH
cord-104282-90t1m430	39	30	terminal	terminal	NN
cord-104282-90t1m430	39	31	signal	signal	NN
cord-104282-90t1m430	39	32	sequence	sequence	NN
cord-104282-90t1m430	39	33	and	and	CC
cord-104282-90t1m430	39	34	a	a	DT
cord-104282-90t1m430	39	35	short	short	JJ
cord-104282-90t1m430	39	36	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	39	37	domain	domain	NN
cord-104282-90t1m430	39	38	at	at	IN
cord-104282-90t1m430	39	39	the	the	DT
cord-104282-90t1m430	39	40	carboxyl	carboxyl	NN
cord-104282-90t1m430	39	41	terminus	terminus	NN
cord-104282-90t1m430	39	42	.	.	.
cord-104282-90t1m430	40	1	We	-PRON-	PRP
cord-104282-90t1m430	40	2	report	report	VBP
cord-104282-90t1m430	40	3	here	here	RB
cord-104282-90t1m430	40	4	that	that	IN
cord-104282-90t1m430	40	5	msALDH	msALDH	NNP
cord-104282-90t1m430	40	6	is	be	VBZ
cord-104282-90t1m430	40	7	localized	localize	VBN
cord-104282-90t1m430	40	8	exclusively	exclusively	RB
cord-104282-90t1m430	40	9	in	in	IN
cord-104282-90t1m430	40	10	the	the	DT
cord-104282-90t1m430	40	11	ER	ER	NNP
cord-104282-90t1m430	40	12	in	in	IN
cord-104282-90t1m430	40	13	COS-1	COS-1	NNP
cord-104282-90t1m430	40	14	cells	cell	NNS
cord-104282-90t1m430	40	15	when	when	WRB
cord-104282-90t1m430	40	16	expressed	express	VBN
cord-104282-90t1m430	40	17	from	from	IN
cord-104282-90t1m430	40	18	cDNA	cdna	NN
cord-104282-90t1m430	40	19	,	,	,
cord-104282-90t1m430	40	20	and	and	CC
cord-104282-90t1m430	40	21	that	that	DT
cord-104282-90t1m430	40	22	expression	expression	NN
cord-104282-90t1m430	40	23	of	of	IN
cord-104282-90t1m430	40	24	this	this	DT
cord-104282-90t1m430	40	25	protein	protein	NN
cord-104282-90t1m430	40	26	apparently	apparently	RB
cord-104282-90t1m430	40	27	alters	alter	VBZ
cord-104282-90t1m430	40	28	the	the	DT
cord-104282-90t1m430	40	29	structure	structure	NN
cord-104282-90t1m430	40	30	of	of	IN
cord-104282-90t1m430	40	31	the	the	DT
cord-104282-90t1m430	40	32	ER	ER	NNP
cord-104282-90t1m430	40	33	from	from	IN
cord-104282-90t1m430	40	34	a	a	DT
cord-104282-90t1m430	40	35	reticular	reticular	NN
cord-104282-90t1m430	40	36	to	to	IN
cord-104282-90t1m430	40	37	large	large	JJ
cord-104282-90t1m430	40	38	vesicular	vesicular	JJ
cord-104282-90t1m430	40	39	one	one	CD
cord-104282-90t1m430	40	40	.	.	.
cord-104282-90t1m430	41	1	In	in	IN
cord-104282-90t1m430	41	2	addition	addition	NN
cord-104282-90t1m430	41	3	,	,	,
cord-104282-90t1m430	41	4	we	-PRON-	PRP
cord-104282-90t1m430	41	5	report	report	VBP
cord-104282-90t1m430	41	6	that	that	IN
cord-104282-90t1m430	41	7	the	the	DT
cord-104282-90t1m430	41	8	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	41	9	domain	domain	NN
cord-104282-90t1m430	41	10	is	be	VBZ
cord-104282-90t1m430	41	11	responsible	responsible	JJ
cord-104282-90t1m430	41	12	for	for	IN
cord-104282-90t1m430	41	13	membrane	membrane	NN
cord-104282-90t1m430	41	14	anchoring	anchor	VBG
cord-104282-90t1m430	41	15	and	and	CC
cord-104282-90t1m430	41	16	that	that	IN
cord-104282-90t1m430	41	17	msALDH	msALDH	NNP
cord-104282-90t1m430	41	18	is	be	VBZ
cord-104282-90t1m430	41	19	likely	likely	JJ
cord-104282-90t1m430	41	20	to	to	TO
cord-104282-90t1m430	41	21	have	have	VB
cord-104282-90t1m430	41	22	two	two	CD
cord-104282-90t1m430	41	23	ER	er	NN
cord-104282-90t1m430	41	24	targeting	target	VBG
cord-104282-90t1m430	41	25	sequences	sequence	NNS
cord-104282-90t1m430	41	26	on	on	IN
cord-104282-90t1m430	41	27	either	either	DT
cord-104282-90t1m430	41	28	side	side	NN
cord-104282-90t1m430	41	29	of	of	IN
cord-104282-90t1m430	41	30	the	the	DT
cord-104282-90t1m430	41	31	membrane	membrane	NN
cord-104282-90t1m430	41	32	anchoring	anchor	VBG
cord-104282-90t1m430	41	33	domain	domain	NN
cord-104282-90t1m430	41	34	.	.	.
cord-104282-90t1m430	42	1	Our	-PRON-	PRP$
cord-104282-90t1m430	42	2	results	result	NNS
cord-104282-90t1m430	42	3	suggest	suggest	VBP
cord-104282-90t1m430	42	4	a	a	DT
cord-104282-90t1m430	42	5	novel	novel	JJ
cord-104282-90t1m430	42	6	mechanism	mechanism	NN
cord-104282-90t1m430	42	7	for	for	IN
cord-104282-90t1m430	42	8	the	the	DT
cord-104282-90t1m430	42	9	posttranslational	posttranslational	JJ
cord-104282-90t1m430	42	10	ER	er	NN
cord-104282-90t1m430	42	11	targeting	targeting	NN
cord-104282-90t1m430	42	12	of	of	IN
cord-104282-90t1m430	42	13	this	this	DT
cord-104282-90t1m430	42	14	tail	tail	NN
cord-104282-90t1m430	42	15	-	-	HYPH
cord-104282-90t1m430	42	16	anchored	anchor	VBN
cord-104282-90t1m430	42	17	protein	protein	NN
cord-104282-90t1m430	42	18	.	.	.
cord-104282-90t1m430	43	1	(	(	-LRB-
cord-104282-90t1m430	43	2	Toyama	Toyama	NNP
cord-104282-90t1m430	43	3	,	,	,
cord-104282-90t1m430	43	4	Japan	Japan	NNP
cord-104282-90t1m430	43	5	)	)	-RRB-
cord-104282-90t1m430	43	6	,	,	,
cord-104282-90t1m430	43	7	respectively	respectively	RB
cord-104282-90t1m430	43	8	.	.	.
cord-104282-90t1m430	44	1	Since	since	IN
cord-104282-90t1m430	44	2	the/3-subunit	the/3-subunit	DT
cord-104282-90t1m430	44	3	of	of	IN
cord-104282-90t1m430	44	4	prolyl	prolyl	NNP
cord-104282-90t1m430	44	5	4-hydroxylase	4-hydroxylase	CD
cord-104282-90t1m430	44	6	has	have	VBZ
cord-104282-90t1m430	44	7	been	be	VBN
cord-104282-90t1m430	44	8	shown	show	VBN
cord-104282-90t1m430	44	9	to	to	TO
cord-104282-90t1m430	44	10	be	be	VB
cord-104282-90t1m430	44	11	identical	identical	JJ
cord-104282-90t1m430	44	12	to	to	IN
cord-104282-90t1m430	44	13	protein	protein	NN
cord-104282-90t1m430	44	14	disulfide	disulfide	NN
cord-104282-90t1m430	44	15	isomerase	isomerase	NN
cord-104282-90t1m430	44	16	(	(	-LRB-
cord-104282-90t1m430	44	17	PDI	PDI	NNP
cord-104282-90t1m430	44	18	)	)	-RRB-
cord-104282-90t1m430	44	19	(	(	-LRB-
cord-104282-90t1m430	44	20	34	34	CD
cord-104282-90t1m430	44	21	)	)	-RRB-
cord-104282-90t1m430	44	22	,	,	,
cord-104282-90t1m430	44	23	this	this	DT
cord-104282-90t1m430	44	24	mAb	mab	NN
cord-104282-90t1m430	44	25	is	be	VBZ
cord-104282-90t1m430	44	26	referred	refer	VBN
cord-104282-90t1m430	44	27	to	to	IN
cord-104282-90t1m430	44	28	as	as	IN
cord-104282-90t1m430	44	29	PDI	PDI	NNP
cord-104282-90t1m430	44	30	mAb	mab	NN
cord-104282-90t1m430	44	31	in	in	IN
cord-104282-90t1m430	44	32	this	this	DT
cord-104282-90t1m430	44	33	paper	paper	NN
cord-104282-90t1m430	44	34	.	.	.
cord-104282-90t1m430	45	1	Rabbit	rabbit	NN
cord-104282-90t1m430	45	2	antibodies	antibody	NNS
cord-104282-90t1m430	45	3	to	to	IN
cord-104282-90t1m430	45	4	rat	rat	NN
cord-104282-90t1m430	46	1	msALDH	msALDH	NNP
cord-104282-90t1m430	46	2	,	,	,
cord-104282-90t1m430	46	3	NADPH	NADPH	NNP
cord-104282-90t1m430	46	4	-	-	HYPH
cord-104282-90t1m430	46	5	cytoehrome	cytoehrome	NNP
cord-104282-90t1m430	47	1	P-450	p-450	CC
cord-104282-90t1m430	47	2	reductase	reductase	NN
cord-104282-90t1m430	47	3	(	(	-LRB-
cord-104282-90t1m430	47	4	FP2	FP2	NNP
cord-104282-90t1m430	47	5	)	)	-RRB-
cord-104282-90t1m430	47	6	,	,	,
cord-104282-90t1m430	47	7	and	and	CC
cord-104282-90t1m430	47	8	PDI	PDI	NNP
cord-104282-90t1m430	47	9	have	have	VBP
cord-104282-90t1m430	47	10	been	be	VBN
cord-104282-90t1m430	47	11	prepared	prepare	VBN
cord-104282-90t1m430	47	12	and	and	CC
cord-104282-90t1m430	47	13	characterized	characterize	VBN
cord-104282-90t1m430	47	14	as	as	IN
cord-104282-90t1m430	47	15	described	describe	VBN
cord-104282-90t1m430	47	16	(	(	-LRB-
cord-104282-90t1m430	47	17	2	2	CD
cord-104282-90t1m430	47	18	,	,	,
cord-104282-90t1m430	47	19	21	21	CD
cord-104282-90t1m430	47	20	,	,	,
cord-104282-90t1m430	47	21	24	24	CD
cord-104282-90t1m430	47	22	)	)	-RRB-
cord-104282-90t1m430	47	23	.	.	.
cord-104282-90t1m430	48	1	Rabbit	Rabbit	NNP
cord-104282-90t1m430	48	2	antiserum	antiserum	NN
cord-104282-90t1m430	48	3	to	to	IN
cord-104282-90t1m430	48	4	bovine	bovine	JJ
cord-104282-90t1m430	48	5	mitochondrial	mitochondrial	NN
cord-104282-90t1m430	48	6	complex	complex	NN
cord-104282-90t1m430	49	1	HI	HI	NNP
cord-104282-90t1m430	49	2	was	be	VBD
cord-104282-90t1m430	49	3	a	a	DT
cord-104282-90t1m430	49	4	generous	generous	JJ
cord-104282-90t1m430	49	5	gift	gift	NN
cord-104282-90t1m430	49	6	from	from	IN
cord-104282-90t1m430	49	7	Dr.	Dr.	NNP
cord-104282-90t1m430	49	8	Takamasa	Takamasa	NNP
cord-104282-90t1m430	49	9	Ozawa	Ozawa	NNP
cord-104282-90t1m430	49	10	(	(	-LRB-
cord-104282-90t1m430	49	11	Nagoya	Nagoya	NNP
cord-104282-90t1m430	49	12	University	University	NNP
cord-104282-90t1m430	49	13	,	,	,
cord-104282-90t1m430	49	14	Nagoya	Nagoya	NNP
cord-104282-90t1m430	49	15	,	,	,
cord-104282-90t1m430	49	16	Japan	Japan	NNP
cord-104282-90t1m430	49	17	)	)	-RRB-
cord-104282-90t1m430	49	18	.	.	.
cord-104282-90t1m430	50	1	The	the	DT
cord-104282-90t1m430	50	2	eukaryotic	eukaryotic	JJ
cord-104282-90t1m430	50	3	assay	assay	NN
cord-104282-90t1m430	50	4	vectors	vector	NNS
cord-104282-90t1m430	50	5	,	,	,
cord-104282-90t1m430	50	6	pCH110	pch110	NN
cord-104282-90t1m430	50	7	and	and	CC
cord-104282-90t1m430	50	8	pSV2CAT	pSV2CAT	NNS
cord-104282-90t1m430	50	9	,	,	,
cord-104282-90t1m430	50	10	were	be	VBD
cord-104282-90t1m430	50	11	from	from	IN
cord-104282-90t1m430	50	12	Pharmaeia	Pharmaeia	NNP
cord-104282-90t1m430	50	13	LKB	LKB	NNP
cord-104282-90t1m430	50	14	Biotechnohigy	Biotechnohigy	NNP
cord-104282-90t1m430	50	15	,	,	,
cord-104282-90t1m430	50	16	Inc.	Inc.	NNP
cord-104282-90t1m430	50	17	(	(	-LRB-
cord-104282-90t1m430	50	18	Uppsala	Uppsala	NNP
cord-104282-90t1m430	50	19	,	,	,
cord-104282-90t1m430	50	20	Sweden	Sweden	NNP
cord-104282-90t1m430	50	21	)	)	-RRB-
cord-104282-90t1m430	50	22	and	and	CC
cord-104282-90t1m430	50	23	Stratagene	Stratagene	NNP
cord-104282-90t1m430	50	24	(	(	-LRB-
cord-104282-90t1m430	50	25	La	La	NNP
cord-104282-90t1m430	50	26	JoUa	JoUa	NNP
cord-104282-90t1m430	50	27	,	,	,
cord-104282-90t1m430	50	28	CA	CA	NNP
cord-104282-90t1m430	50	29	)	)	-RRB-
cord-104282-90t1m430	50	30	,	,	,
cord-104282-90t1m430	50	31	respectively	respectively	RB
cord-104282-90t1m430	50	32	.	.	.
cord-104282-90t1m430	51	1	The	the	DT
cord-104282-90t1m430	51	2	eukaryotic	eukaryotic	JJ
cord-104282-90t1m430	51	3	expression	expression	NN
cord-104282-90t1m430	51	4	vector	vector	NN
cord-104282-90t1m430	51	5	pMIW	pmiw	NN
cord-104282-90t1m430	51	6	(	(	-LRB-
cord-104282-90t1m430	51	7	43	43	CD
cord-104282-90t1m430	51	8	)	)	-RRB-
cord-104282-90t1m430	51	9	was	be	VBD
cord-104282-90t1m430	51	10	kindly	kindly	RB
cord-104282-90t1m430	51	11	provided	provide	VBN
cord-104282-90t1m430	51	12	by	by	IN
cord-104282-90t1m430	51	13	Dr.	Dr.	NNP
cord-104282-90t1m430	51	14	Akihiro	Akihiro	NNP
cord-104282-90t1m430	51	15	Inoue	Inoue	NNP
cord-104282-90t1m430	51	16	(	(	-LRB-
cord-104282-90t1m430	51	17	National	National	NNP
cord-104282-90t1m430	51	18	Institute	Institute	NNP
cord-104282-90t1m430	51	19	for	for	IN
cord-104282-90t1m430	51	20	Physiological	Physiological	NNP
cord-104282-90t1m430	51	21	Sciences	Sciences	NNPS
cord-104282-90t1m430	51	22	,	,	,
cord-104282-90t1m430	51	23	Okazaki	Okazaki	NNP
cord-104282-90t1m430	51	24	,	,	,
cord-104282-90t1m430	51	25	Japan	Japan	NNP
cord-104282-90t1m430	51	26	)	)	-RRB-
cord-104282-90t1m430	51	27	.	.	.
cord-104282-90t1m430	52	1	Restriction	restriction	NN
cord-104282-90t1m430	52	2	enzymes	enzyme	NNS
cord-104282-90t1m430	52	3	and	and	CC
cord-104282-90t1m430	52	4	DNA	dna	NN
cord-104282-90t1m430	52	5	modifying	modify	VBG
cord-104282-90t1m430	52	6	enzymes	enzyme	NNS
cord-104282-90t1m430	52	7	were	be	VBD
cord-104282-90t1m430	52	8	purchased	purchase	VBN
cord-104282-90t1m430	52	9	from	from	IN
cord-104282-90t1m430	52	10	Nippon	Nippon	NNP
cord-104282-90t1m430	52	11	Gene	Gene	NNP
cord-104282-90t1m430	52	12	(	(	-LRB-
cord-104282-90t1m430	52	13	Toyama	Toyama	NNP
cord-104282-90t1m430	52	14	,	,	,
cord-104282-90t1m430	52	15	Japan	Japan	NNP
cord-104282-90t1m430	52	16	)	)	-RRB-
cord-104282-90t1m430	52	17	and	and	CC
cord-104282-90t1m430	52	18	Takara	Takara	NNP
cord-104282-90t1m430	52	19	Co.	Co.	NNP
cord-104282-90t1m430	52	20	,	,	,
cord-104282-90t1m430	52	21	Ltd.	Ltd.	NNP
cord-104282-90t1m430	52	22	(	(	-LRB-
cord-104282-90t1m430	52	23	Kyoto	Kyoto	NNP
cord-104282-90t1m430	52	24	,	,	,
cord-104282-90t1m430	52	25	Japan	Japan	NNP
cord-104282-90t1m430	52	26	)	)	-RRB-
cord-104282-90t1m430	52	27	.	.	.
cord-104282-90t1m430	53	1	All	all	DT
cord-104282-90t1m430	53	2	other	other	JJ
cord-104282-90t1m430	53	3	chemicals	chemical	NNS
cord-104282-90t1m430	53	4	were	be	VBD
cord-104282-90t1m430	53	5	of	of	IN
cord-104282-90t1m430	53	6	the	the	DT
cord-104282-90t1m430	53	7	highest	high	JJS
cord-104282-90t1m430	53	8	purity	purity	NN
cord-104282-90t1m430	53	9	commercially	commercially	RB
cord-104282-90t1m430	53	10	available	available	JJ
cord-104282-90t1m430	53	11	.	.	.
cord-104282-90t1m430	54	1	All	all	DT
cord-104282-90t1m430	54	2	constructions	construction	NNS
cord-104282-90t1m430	54	3	were	be	VBD
cord-104282-90t1m430	54	4	verified	verify	VBN
cord-104282-90t1m430	54	5	by	by	IN
cord-104282-90t1m430	54	6	the	the	DT
cord-104282-90t1m430	54	7	dideoxy	dideoxy	NN
cord-104282-90t1m430	54	8	chain	chain	NN
cord-104282-90t1m430	54	9	termination	termination	NN
cord-104282-90t1m430	54	10	method	method	NN
cord-104282-90t1m430	54	11	(	(	-LRB-
cord-104282-90t1m430	54	12	38	38	CD
cord-104282-90t1m430	54	13	)	)	-RRB-
cord-104282-90t1m430	54	14	.	.	.
cord-104282-90t1m430	55	1	Insertion	insertion	NN
cord-104282-90t1m430	55	2	of	of	IN
cord-104282-90t1m430	55	3	a	a	DT
cord-104282-90t1m430	55	4	full	full	JJ
cord-104282-90t1m430	55	5	-	-	HYPH
cord-104282-90t1m430	55	6	length	length	NN
cord-104282-90t1m430	55	7	eDNA	edna	NN
cord-104282-90t1m430	55	8	encoding	encode	VBG
cord-104282-90t1m430	55	9	rat	rat	NN
cord-104282-90t1m430	55	10	msALDH	msALDH	VBN
cord-104282-90t1m430	55	11	into	into	IN
cord-104282-90t1m430	55	12	the	the	DT
cord-104282-90t1m430	55	13	unique	unique	JJ
cord-104282-90t1m430	55	14	EcoRI	ecori	NN
cord-104282-90t1m430	55	15	site	site	NN
cord-104282-90t1m430	55	16	of	of	IN
cord-104282-90t1m430	55	17	the	the	DT
cord-104282-90t1m430	55	18	SV-40-based	sv-40-based	JJ
cord-104282-90t1m430	55	19	vector	vector	NN
cord-104282-90t1m430	55	20	pCD	pCD	NNP
cord-104282-90t1m430	55	21	(	(	-LRB-
cord-104282-90t1m430	55	22	30	30	CD
cord-104282-90t1m430	55	23	)	)	-RRB-
cord-104282-90t1m430	55	24	has	have	VBZ
cord-104282-90t1m430	55	25	been	be	VBN
cord-104282-90t1m430	55	26	described	describe	VBN
cord-104282-90t1m430	55	27	previously	previously	RB
cord-104282-90t1m430	55	28	(	(	-LRB-
cord-104282-90t1m430	55	29	24	24	CD
cord-104282-90t1m430	55	30	)	)	-RRB-
cord-104282-90t1m430	55	31	.	.	.
cord-104282-90t1m430	56	1	The	the	DT
cord-104282-90t1m430	56	2	gapped	gap	VBN
cord-104282-90t1m430	56	3	duplex	duplex	NN
cord-104282-90t1m430	56	4	method	method	NN
cord-104282-90t1m430	56	5	of	of	IN
cord-104282-90t1m430	56	6	oligonueleotide	oligonueleotide	RB
cord-104282-90t1m430	56	7	-	-	HYPH
cord-104282-90t1m430	56	8	directed	direct	VBN
cord-104282-90t1m430	56	9	mutagenesis	mutagenesis	NN
cord-104282-90t1m430	56	10	(	(	-LRB-
cord-104282-90t1m430	56	11	19	19	CD
cord-104282-90t1m430	56	12	)	)	-RRB-
cord-104282-90t1m430	57	1	A	a	DT
cord-104282-90t1m430	57	2	chimeric	chimeric	JJ
cord-104282-90t1m430	57	3	eDNA	edna	NN
cord-104282-90t1m430	57	4	for	for	IN
cord-104282-90t1m430	57	5	E.	E.	NNP
cord-104282-90t1m430	57	6	coli/~-gaiactosidase	coli/~-gaiactosidase	NNP
cord-104282-90t1m430	58	1	fusion	fusion	NN
cord-104282-90t1m430	58	2	protein	protein	NN
cord-104282-90t1m430	58	3	was	be	VBD
cord-104282-90t1m430	58	4	constructed	construct	VBN
cord-104282-90t1m430	58	5	as	as	IN
cord-104282-90t1m430	58	6	follows	follow	NNS
cord-104282-90t1m430	58	7	.	.	.
cord-104282-90t1m430	59	1	First	first	RB
cord-104282-90t1m430	59	2	,	,	,
cord-104282-90t1m430	59	3	a	a	DT
cord-104282-90t1m430	59	4	DNA	dna	NN
cord-104282-90t1m430	59	5	fragment	fragment	NN
cord-104282-90t1m430	59	6	encoding	encode	VBG
cord-104282-90t1m430	59	7	the	the	DT
cord-104282-90t1m430	59	8	earboxyl	earboxyl	NN
cord-104282-90t1m430	59	9	-	-	HYPH
cord-104282-90t1m430	59	10	terminal	terminal	NN
cord-104282-90t1m430	59	11	53	53	CD
cord-104282-90t1m430	59	12	amino	amino	NN
cord-104282-90t1m430	59	13	acids	acid	NNS
cord-104282-90t1m430	59	14	(	(	-LRB-
cord-104282-90t1m430	59	15	amino	amino	JJ
cord-104282-90t1m430	59	16	acids	acid	NNS
cord-104282-90t1m430	59	17	994	994	CD
cord-104282-90t1m430	59	18	-	-	HYPH
cord-104282-90t1m430	59	19	1046	1046	CD
cord-104282-90t1m430	59	20	)	)	-RRB-
cord-104282-90t1m430	60	1	of/5-galaetosidase	of/5-galaetosidase	NNP
cord-104282-90t1m430	60	2	,	,	,
cord-104282-90t1m430	60	3	followed	follow	VBN
cord-104282-90t1m430	60	4	by	by	IN
cord-104282-90t1m430	60	5	five	five	CD
cord-104282-90t1m430	60	6	amino	amino	NN
cord-104282-90t1m430	60	7	acids	acid	NNS
cord-104282-90t1m430	60	8	(	(	-LRB-
cord-104282-90t1m430	60	9	amino	amino	NN
cord-104282-90t1m430	60	10	acids	acid	NNS
cord-104282-90t1m430	60	11	450	450	CD
cord-104282-90t1m430	60	12	-	-	SYM
cord-104282-90t1m430	60	13	454	454	CD
cord-104282-90t1m430	60	14	)	)	-RRB-
cord-104282-90t1m430	60	15	of	of	IN
cord-104282-90t1m430	60	16	msALDH	msALDH	NNP
cord-104282-90t1m430	60	17	,	,	,
cord-104282-90t1m430	60	18	was	be	VBD
cord-104282-90t1m430	60	19	created	create	VBN
cord-104282-90t1m430	60	20	by	by	IN
cord-104282-90t1m430	60	21	PCR	PCR	NNP
cord-104282-90t1m430	60	22	using	use	VBG
cord-104282-90t1m430	60	23	oligonuclcotides	oligonuclcotides	NNP
cord-104282-90t1m430	60	24	no	no	NN
cord-104282-90t1m430	60	25	.	.	NN
cord-104282-90t1m430	60	26	6	6	CD
cord-104282-90t1m430	60	27	(	(	-LRB-
cord-104282-90t1m430	60	28	5	5	CD
cord-104282-90t1m430	60	29	'	'	``
cord-104282-90t1m430	60	30	AGCCATCGCCATCTGCTG	AGCCATCGCCATCTGCTG	NNP
cord-104282-90t1m430	60	31	3	3	NNP
cord-104282-90t1m430	60	32	'	'	''
cord-104282-90t1m430	60	33	)	)	-RRB-
cord-104282-90t1m430	60	34	and	and	CC
cord-104282-90t1m430	60	35	no	no	NN
cord-104282-90t1m430	60	36	.	.	NN
cord-104282-90t1m430	60	37	7	7	CD
cord-104282-90t1m430	60	38	(	(	-LRB-
cord-104282-90t1m430	60	39	5	5	CD
cord-104282-90t1m430	60	40	'	'	''
cord-104282-90t1m430	60	41	GGAAGAATTTCGACCATrTTW_tACACCAGACC	GGAAGAATTTCGACCATrTTW_tACACCAGACC	NNP
cord-104282-90t1m430	60	42	3	3	CD
cord-104282-90t1m430	60	43	'	'	''
cord-104282-90t1m430	60	44	)	)	-RRB-
cord-104282-90t1m430	60	45	.	.	.
cord-104282-90t1m430	61	1	Similarly	similarly	RB
cord-104282-90t1m430	61	2	,	,	,
cord-104282-90t1m430	61	3	a	a	DT
cord-104282-90t1m430	61	4	second	second	JJ
cord-104282-90t1m430	61	5	DNA	dna	NN
cord-104282-90t1m430	61	6	fragment	fragment	NN
cord-104282-90t1m430	61	7	corresponding	correspond	VBG
cord-104282-90t1m430	61	8	to	to	IN
cord-104282-90t1m430	61	9	the	the	DT
cord-104282-90t1m430	61	10	last	last	JJ
cord-104282-90t1m430	61	11	five	five	CD
cord-104282-90t1m430	61	12	amino	amino	NN
cord-104282-90t1m430	61	13	acids	acid	NNS
cord-104282-90t1m430	61	14	(	(	-LRB-
cord-104282-90t1m430	61	15	amino	amino	JJ
cord-104282-90t1m430	61	16	acids	acid	NNS
cord-104282-90t1m430	61	17	1042	1042	CD
cord-104282-90t1m430	61	18	-	-	HYPH
cord-104282-90t1m430	61	19	1046	1046	CD
cord-104282-90t1m430	61	20	)	)	-RRB-
cord-104282-90t1m430	62	1	of/5-galactosidase	of/5-galactosidase	NNP
cord-104282-90t1m430	62	2	followed	follow	VBN
cord-104282-90t1m430	62	3	by	by	IN
cord-104282-90t1m430	62	4	the	the	DT
cord-104282-90t1m430	62	5	last	last	JJ
cord-104282-90t1m430	62	6	35	35	CD
cord-104282-90t1m430	62	7	amino	amino	NN
cord-104282-90t1m430	62	8	acids	acid	NNS
cord-104282-90t1m430	62	9	(	(	-LRB-
cord-104282-90t1m430	62	10	amino	amino	NN
cord-104282-90t1m430	62	11	acids	acid	NNS
cord-104282-90t1m430	62	12	450	450	CD
cord-104282-90t1m430	62	13	-	-	SYM
cord-104282-90t1m430	62	14	484	484	CD
cord-104282-90t1m430	62	15	)	)	-RRB-
cord-104282-90t1m430	62	16	and	and	CC
cord-104282-90t1m430	63	1	3	3	LS
cord-104282-90t1m430	63	2	'	'	POS
cord-104282-90t1m430	63	3	untranslated	untranslated	JJ
cord-104282-90t1m430	63	4	sequence	sequence	NN
cord-104282-90t1m430	63	5	of	of	IN
cord-104282-90t1m430	63	6	msALDH	msALDH	NNP
cord-104282-90t1m430	63	7	was	be	VBD
cord-104282-90t1m430	63	8	amplified	amplify	VBN
cord-104282-90t1m430	63	9	using	use	VBG
cord-104282-90t1m430	63	10	oligonueleotides	oligonueleotide	NNS
cord-104282-90t1m430	63	11	no	no	UH
cord-104282-90t1m430	63	12	.	.	.
cord-104282-90t1m430	64	1	8	8	CD
cord-104282-90t1m430	65	1	(	(	-LRB-
cord-104282-90t1m430	65	2	5	5	CD
cord-104282-90t1m430	65	3	'	'	''
cord-104282-90t1m430	65	4	C_~'ICTC.~TG'IC_AAAAA'IUGTCGAAAT	C_~'ICTC.~TG'IC_AAAAA'IUGTCGAAAT	NNP
cord-104282-90t1m430	65	5	-	-	HYPH
cord-104282-90t1m430	65	6	TCTICC	TCTICC	NNP
cord-104282-90t1m430	65	7	3	3	NNP
cord-104282-90t1m430	65	8	'	'	''
cord-104282-90t1m430	65	9	)	)	-RRB-
cord-104282-90t1m430	65	10	and	and	CC
cord-104282-90t1m430	65	11	no	no	UH
cord-104282-90t1m430	65	12	.	.	NN
cord-104282-90t1m430	65	13	9	9	CD
cord-104282-90t1m430	65	14	with	with	IN
cord-104282-90t1m430	65	15	mutated	mutate	VBN
cord-104282-90t1m430	65	16	nucleotides	nucleotide	NNS
cord-104282-90t1m430	65	17	underlined	underline	VBN
cord-104282-90t1m430	65	18	for	for	IN
cord-104282-90t1m430	65	19	introduction	introduction	NN
cord-104282-90t1m430	65	20	of	of	IN
cord-104282-90t1m430	65	21	an	an	DT
cord-104282-90t1m430	65	22	EcoRI	ecori	NN
cord-104282-90t1m430	65	23	site	site	NN
cord-104282-90t1m430	65	24	(	(	-LRB-
cord-104282-90t1m430	65	25	5	5	CD
cord-104282-90t1m430	65	26	'	'	''
cord-104282-90t1m430	65	27	AACAACTTAGAATTCAC	AACAACTTAGAATTCAC	NNP
cord-104282-90t1m430	65	28	~	~	NFP
cord-104282-90t1m430	65	29	GTTC	GTTC	NNP
cord-104282-90t1m430	65	30	3	3	NNP
cord-104282-90t1m430	65	31	'	'	''
cord-104282-90t1m430	65	32	)	)	-RRB-
cord-104282-90t1m430	65	33	.	.	.
cord-104282-90t1m430	66	1	These	these	DT
cord-104282-90t1m430	66	2	two	two	CD
cord-104282-90t1m430	66	3	fragments	fragment	NNS
cord-104282-90t1m430	66	4	were	be	VBD
cord-104282-90t1m430	66	5	then	then	RB
cord-104282-90t1m430	66	6	used	use	VBN
cord-104282-90t1m430	66	7	as	as	IN
cord-104282-90t1m430	66	8	the	the	DT
cord-104282-90t1m430	66	9	templates	template	NNS
cord-104282-90t1m430	66	10	to	to	TO
cord-104282-90t1m430	66	11	create	create	VB
cord-104282-90t1m430	66	12	a	a	DT
cord-104282-90t1m430	66	13	chimeric	chimeric	JJ
cord-104282-90t1m430	66	14	eDNA	edna	NN
cord-104282-90t1m430	66	15	with	with	IN
cord-104282-90t1m430	66	16	the	the	DT
cord-104282-90t1m430	66	17	oligonucleotides	oligonucleotide	NNS
cord-104282-90t1m430	66	18	nos	nos	NNP
cord-104282-90t1m430	66	19	.	.	NNP
cord-104282-90t1m430	66	20	6	6	CD
cord-104282-90t1m430	66	21	and	and	CC
cord-104282-90t1m430	66	22	9	9	CD
cord-104282-90t1m430	66	23	.	.	.
cord-104282-90t1m430	67	1	The	the	DT
cord-104282-90t1m430	67	2	resultant	resultant	JJ
cord-104282-90t1m430	67	3	PCR	pcr	NN
cord-104282-90t1m430	67	4	fragment	fragment	NN
cord-104282-90t1m430	67	5	was	be	VBD
cord-104282-90t1m430	67	6	digested	digest	VBN
cord-104282-90t1m430	67	7	with	with	IN
cord-104282-90t1m430	67	8	EcoRI	ecori	NN
cord-104282-90t1m430	67	9	and	and	CC
cord-104282-90t1m430	67	10	ligated	ligate	VBN
cord-104282-90t1m430	67	11	in	in	IN
cord-104282-90t1m430	67	12	-	-	HYPH
cord-104282-90t1m430	67	13	frame	frame	NN
cord-104282-90t1m430	67	14	to	to	IN
cord-104282-90t1m430	67	15	the	the	DT
cord-104282-90t1m430	67	16	EcoRI	ecori	NN
cord-104282-90t1m430	67	17	site	site	NN
cord-104282-90t1m430	67	18	(	(	-LRB-
cord-104282-90t1m430	67	19	amino	amino	JJ
cord-104282-90t1m430	67	20	acids	acid	NNS
cord-104282-90t1m430	67	21	1029	1029	CD
cord-104282-90t1m430	67	22	-	-	SYM
cord-104282-90t1m430	67	23	1030	1030	CD
cord-104282-90t1m430	67	24	)	)	-RRB-
cord-104282-90t1m430	67	25	of	of	IN
cord-104282-90t1m430	67	26	pCHII0	pCHII0	NNP
cord-104282-90t1m430	67	27	to	to	TO
cord-104282-90t1m430	67	28	construct	construct	VB
cord-104282-90t1m430	67	29	pCHII0	pCHII0	NNP
cord-104282-90t1m430	67	30	/	/	SYM
cord-104282-90t1m430	67	31	ALDH	ALDH	NNP
cord-104282-90t1m430	67	32	.	.	.
cord-104282-90t1m430	68	1	Since	since	IN
cord-104282-90t1m430	68	2	it	-PRON-	PRP
cord-104282-90t1m430	68	3	was	be	VBD
cord-104282-90t1m430	68	4	difficult	difficult	JJ
cord-104282-90t1m430	68	5	to	to	TO
cord-104282-90t1m430	68	6	construct	construct	VB
cord-104282-90t1m430	68	7	a	a	DT
cord-104282-90t1m430	68	8	series	series	NN
cord-104282-90t1m430	68	9	of	of	IN
cord-104282-90t1m430	68	10	various	various	JJ
cord-104282-90t1m430	68	11	fusion	fusion	NN
cord-104282-90t1m430	68	12	genes	gene	NNS
cord-104282-90t1m430	68	13	using	use	VBG
cord-104282-90t1m430	68	14	~-gaiactosidase	~-gaiactosidase	NFP
cord-104282-90t1m430	69	1	eDNA	eDNA	NNP
cord-104282-90t1m430	70	1	according	accord	VBG
cord-104282-90t1m430	70	2	to	to	IN
cord-104282-90t1m430	70	3	the	the	DT
cord-104282-90t1m430	70	4	method	method	NN
cord-104282-90t1m430	70	5	described	describe	VBN
cord-104282-90t1m430	70	6	above	above	RB
cord-104282-90t1m430	70	7	,	,	,
cord-104282-90t1m430	70	8	we	-PRON-	PRP
cord-104282-90t1m430	70	9	used	use	VBD
cord-104282-90t1m430	70	10	CAT	cat	NN
cord-104282-90t1m430	70	11	eDNA	edna	NN
cord-104282-90t1m430	71	1	for	for	IN
cord-104282-90t1m430	71	2	further	further	JJ
cord-104282-90t1m430	71	3	analyses	analysis	NNS
cord-104282-90t1m430	71	4	on	on	IN
cord-104282-90t1m430	71	5	the	the	DT
cord-104282-90t1m430	71	6	role	role	NN
cord-104282-90t1m430	71	7	of	of	IN
cord-104282-90t1m430	71	8	the	the	DT
cord-104282-90t1m430	71	9	carboxyl	carboxyl	NN
cord-104282-90t1m430	71	10	-	-	HYPH
cord-104282-90t1m430	71	11	terminai	terminai	NN
cord-104282-90t1m430	71	12	portion	portion	NN
cord-104282-90t1m430	71	13	of	of	IN
cord-104282-90t1m430	71	14	msALDH	msALDH	NNP
cord-104282-90t1m430	71	15	in	in	IN
cord-104282-90t1m430	71	16	the	the	DT
cord-104282-90t1m430	71	17	intracellular	intracellular	JJ
cord-104282-90t1m430	71	18	localization	localization	NN
cord-104282-90t1m430	71	19	.	.	.
cord-104282-90t1m430	72	1	Chimeric	chimeric	JJ
cord-104282-90t1m430	72	2	cDNAs	cdna	NNS
cord-104282-90t1m430	72	3	for	for	IN
cord-104282-90t1m430	72	4	CAT	cat	NN
cord-104282-90t1m430	72	5	fusion	fusion	NN
cord-104282-90t1m430	72	6	proteins	protein	NNS
cord-104282-90t1m430	72	7	(	(	-LRB-
cord-104282-90t1m430	72	8	CAT	CAT	NNP
cord-104282-90t1m430	72	9	/	/	SYM
cord-104282-90t1m430	72	10	ALDH	ALDH	NNP
cord-104282-90t1m430	72	11	chimeras	chimera	NNS
cord-104282-90t1m430	72	12	)	)	-RRB-
cord-104282-90t1m430	72	13	were	be	VBD
cord-104282-90t1m430	72	14	constructed	construct	VBN
cord-104282-90t1m430	72	15	essentially	essentially	RB
cord-104282-90t1m430	72	16	by	by	IN
cord-104282-90t1m430	72	17	combination	combination	NN
cord-104282-90t1m430	72	18	of	of	IN
cord-104282-90t1m430	72	19	oligonucleotide	oligonucleotide	RB
cord-104282-90t1m430	72	20	-	-	HYPH
cord-104282-90t1m430	72	21	directed	direct	VBN
cord-104282-90t1m430	72	22	mutagenesis	mutagenesis	NN
cord-104282-90t1m430	72	23	and	and	CC
cord-104282-90t1m430	72	24	PCR	PCR	NNP
cord-104282-90t1m430	72	25	.	.	.
cord-104282-90t1m430	73	1	A	a	DT
cord-104282-90t1m430	73	2	1.8-kbp	1.8-kbp	CD
cord-104282-90t1m430	73	3	HindHLBamHI	hindhlbamhi	NN
cord-104282-90t1m430	73	4	eDNA	edna	NN
cord-104282-90t1m430	73	5	fragment	fragment	NN
cord-104282-90t1m430	73	6	encoding	encode	VBG
cord-104282-90t1m430	73	7	CAT	cat	NN
cord-104282-90t1m430	73	8	in	in	IN
cord-104282-90t1m430	73	9	the	the	DT
cord-104282-90t1m430	73	10	pSV2CAT	pSV2CAT	NNS
cord-104282-90t1m430	73	11	was	be	VBD
cord-104282-90t1m430	73	12	cloned	clone	VBN
cord-104282-90t1m430	73	13	into	into	IN
cord-104282-90t1m430	73	14	Ml3tvl8	Ml3tvl8	NNP
cord-104282-90t1m430	73	15	.	.	.
cord-104282-90t1m430	74	1	Oligonuclnotides	Oligonuclnotides	NNP
cord-104282-90t1m430	74	2	no	no	RB
cord-104282-90t1m430	74	3	.	.	.
cord-104282-90t1m430	75	1	10	10	CD
cord-104282-90t1m430	75	2	(	(	-LRB-
cord-104282-90t1m430	75	3	5	5	CD
cord-104282-90t1m430	75	4	'	'	''
cord-104282-90t1m430	75	5	AG	AG	NNP
cord-104282-90t1m430	75	6	~	~	NFP
cord-104282-90t1m430	75	7	GGC	GGC	NNP
cord-104282-90t1m430	75	8	~	~	NFP
cord-104282-90t1m430	75	9	GGTACCT	GGTACCT	NNP
cord-104282-90t1m430	75	10	-	-	HYPH
cord-104282-90t1m430	75	11	AATTTTTTTA	AATTTTTTTA	NNP
cord-104282-90t1m430	75	12	3	3	NNP
cord-104282-90t1m430	75	13	'	'	''
cord-104282-90t1m430	75	14	)	)	-RRB-
cord-104282-90t1m430	76	1	and	and	CC
cord-104282-90t1m430	76	2	no	no	UH
cord-104282-90t1m430	76	3	.	.	NN
cord-104282-90t1m430	76	4	11	11	CD
cord-104282-90t1m430	76	5	(	(	-LRB-
cord-104282-90t1m430	76	6	5	5	CD
cord-104282-90t1m430	76	7	'	'	``
cord-104282-90t1m430	76	8	ATAAGTGATATCAAGCGGATGA	ATAAGTGATATCAAGCGGATGA	NNP
cord-104282-90t1m430	76	9	3	3	NNP
cord-104282-90t1m430	76	10	'	'	''
cord-104282-90t1m430	76	11	)	)	-RRB-
cord-104282-90t1m430	76	12	with	with	IN
cord-104282-90t1m430	76	13	mutated	mutate	VBN
cord-104282-90t1m430	76	14	nucleotides	nucleotide	NNS
cord-104282-90t1m430	76	15	underlined	underline	VBN
cord-104282-90t1m430	76	16	were	be	VBD
cord-104282-90t1m430	76	17	used	use	VBN
cord-104282-90t1m430	76	18	for	for	IN
cord-104282-90t1m430	76	19	generation	generation	NN
cord-104282-90t1m430	76	20	of	of	IN
cord-104282-90t1m430	76	21	a	a	DT
cord-104282-90t1m430	76	22	KpnI	KpnI	NNP
cord-104282-90t1m430	76	23	site	site	NN
cord-104282-90t1m430	76	24	at	at	IN
cord-104282-90t1m430	76	25	the	the	DT
cord-104282-90t1m430	76	26	carboxyl	carboxyl	NN
cord-104282-90t1m430	76	27	terminus	terminus	NN
cord-104282-90t1m430	76	28	and	and	CC
cord-104282-90t1m430	76	29	an	an	DT
cord-104282-90t1m430	76	30	EcoRV	EcoRV	NNP
cord-104282-90t1m430	76	31	site	site	NN
cord-104282-90t1m430	76	32	in	in	IN
cord-104282-90t1m430	76	33	the	the	DT
cord-104282-90t1m430	76	34	3	3	CD
cord-104282-90t1m430	76	35	'	'	POS
cord-104282-90t1m430	76	36	untranslated	untranslated	JJ
cord-104282-90t1m430	76	37	sequence	sequence	NN
cord-104282-90t1m430	76	38	of	of	IN
cord-104282-90t1m430	76	39	CAT	cat	NN
cord-104282-90t1m430	76	40	,	,	,
cord-104282-90t1m430	76	41	respectively	respectively	RB
cord-104282-90t1m430	76	42	.	.	.
cord-104282-90t1m430	77	1	The	the	DT
cord-104282-90t1m430	77	2	mutated	mutate	VBN
cord-104282-90t1m430	77	3	eDNA	edna	NN
cord-104282-90t1m430	77	4	was	be	VBD
cord-104282-90t1m430	77	5	cloned	clone	VBN
cord-104282-90t1m430	77	6	into	into	IN
cord-104282-90t1m430	77	7	HindlH/	hindlh/	JJ
cord-104282-90t1m430	77	8	BamHI	bamhi	NN
cord-104282-90t1m430	77	9	-	-	HYPH
cord-104282-90t1m430	77	10	digested	digest	VBN
cord-104282-90t1m430	77	11	pMIW	pmiw	JJ
cord-104282-90t1m430	77	12	expression	expression	NN
cord-104282-90t1m430	77	13	vector	vector	NN
cord-104282-90t1m430	77	14	to	to	TO
cord-104282-90t1m430	77	15	construct	construct	VB
cord-104282-90t1m430	77	16	pMIWCAT	pmiwcat	NN
cord-104282-90t1m430	77	17	.	.	.
cord-104282-90t1m430	78	1	PCR	PCR	NNP
cord-104282-90t1m430	78	2	was	be	VBD
cord-104282-90t1m430	78	3	then	then	RB
cord-104282-90t1m430	78	4	used	use	VBN
cord-104282-90t1m430	78	5	to	to	TO
cord-104282-90t1m430	78	6	generate	generate	VB
cord-104282-90t1m430	78	7	DNA	dna	NN
cord-104282-90t1m430	78	8	fragments	fragment	NNS
cord-104282-90t1m430	78	9	corresponding	correspond	VBG
cord-104282-90t1m430	78	10	to	to	IN
cord-104282-90t1m430	78	11	the	the	DT
cord-104282-90t1m430	78	12	carboxylterminal	carboxylterminal	JJ
cord-104282-90t1m430	78	13	regions	region	NNS
cord-104282-90t1m430	78	14	of	of	IN
cord-104282-90t1m430	78	15	msALDH	msALDH	NNP
cord-104282-90t1m430	78	16	,	,	,
cord-104282-90t1m430	78	17	including	include	VBG
cord-104282-90t1m430	78	18	the	the	DT
cord-104282-90t1m430	78	19	3	3	CD
cord-104282-90t1m430	78	20	'	'	POS
cord-104282-90t1m430	78	21	untranslated	untranslated	JJ
cord-104282-90t1m430	78	22	sequence	sequence	NN
cord-104282-90t1m430	78	23	.	.	.
cord-104282-90t1m430	79	1	PCR	PCR	NNP
cord-104282-90t1m430	79	2	reactions	reaction	NNS
cord-104282-90t1m430	79	3	used	use	VBD
cord-104282-90t1m430	79	4	the	the	DT
cord-104282-90t1m430	79	5	following	follow	VBG
cord-104282-90t1m430	79	6	primers	primer	NNS
cord-104282-90t1m430	79	7	and	and	CC
cord-104282-90t1m430	79	8	templates	template	NNS
cord-104282-90t1m430	79	9	to	to	TO
cord-104282-90t1m430	79	10	amplify	amplify	VB
cord-104282-90t1m430	79	11	DNA	dna	NN
cord-104282-90t1m430	79	12	fragments	fragment	NNS
cord-104282-90t1m430	79	13	termed	term	VBN
cord-104282-90t1m430	79	14	ALDH1	ALDH1	NNP
cord-104282-90t1m430	79	15	-	-	HYPH
cord-104282-90t1m430	79	16	7	7	CD
cord-104282-90t1m430	79	17	and	and	CC
cord-104282-90t1m430	79	18	9	9	CD
cord-104282-90t1m430	79	19	:	:	:
cord-104282-90t1m430	79	20	ALDH1	ALDH1	NNP
cord-104282-90t1m430	79	21	;	;	:
cord-104282-90t1m430	79	22	oligonueleotides	oligonueleotides	NNP
cord-104282-90t1m430	79	23	no	no	UH
cord-104282-90t1m430	79	24	.	.	.
cord-104282-90t1m430	80	1	12	12	CD
cord-104282-90t1m430	80	2	(	(	-LRB-
cord-104282-90t1m430	80	3	5	5	CD
cord-104282-90t1m430	80	4	'	'	POS
cord-104282-90t1m430	80	5	GAGTCCAAGGGTACCTGGTCGAAATTC	GAGTCCAAGGGTACCTGGTCGAAATTC	NNP
cord-104282-90t1m430	80	6	3	3	CD
cord-104282-90t1m430	80	7	'	'	''
cord-104282-90t1m430	80	8	)	)	-RRB-
cord-104282-90t1m430	80	9	with	with	IN
cord-104282-90t1m430	80	10	mutated	mutate	VBN
cord-104282-90t1m430	80	11	nueicotides	nueicotide	NNS
cord-104282-90t1m430	80	12	underlined	underline	VBN
cord-104282-90t1m430	80	13	for	for	IN
cord-104282-90t1m430	80	14	introduction	introduction	NN
cord-104282-90t1m430	80	15	of	of	IN
cord-104282-90t1m430	80	16	a	a	DT
cord-104282-90t1m430	80	17	KpnI	KpnI	NNP
cord-104282-90t1m430	80	18	site	site	NN
cord-104282-90t1m430	80	19	and	and	CC
cord-104282-90t1m430	80	20	no	no	NN
cord-104282-90t1m430	80	21	.	.	NN
cord-104282-90t1m430	80	22	9	9	CD
cord-104282-90t1m430	80	23	(	(	-LRB-
cord-104282-90t1m430	80	24	pCDALDH	pCDALDH	NNP
cord-104282-90t1m430	80	25	as	as	IN
cord-104282-90t1m430	80	26	template	template	NN
cord-104282-90t1m430	80	27	)	)	-RRB-
cord-104282-90t1m430	80	28	,	,	,
cord-104282-90t1m430	80	29	ALDI	ALDI	NNP
cord-104282-90t1m430	80	30	-	-	HYPH
cord-104282-90t1m430	80	31	I2	I2	NNP
cord-104282-90t1m430	80	32	;	;	:
cord-104282-90t1m430	80	33	oligonucleotides	oligonucleotide	NNS
cord-104282-90t1m430	80	34	no	no	RB
cord-104282-90t1m430	80	35	.	.	.
cord-104282-90t1m430	81	1	12	12	CD
cord-104282-90t1m430	81	2	and	and	CC
cord-104282-90t1m430	81	3	no	no	UH
cord-104282-90t1m430	81	4	.	.	.
cord-104282-90t1m430	82	1	9	9	LS
cord-104282-90t1m430	82	2	(	(	-LRB-
cord-104282-90t1m430	82	3	pCDALDHA481	pcdaldha481	VB
cord-104282-90t1m430	82	4	-	-	SYM
cord-104282-90t1m430	82	5	484	484	CD
cord-104282-90t1m430	82	6	as	as	IN
cord-104282-90t1m430	82	7	template	template	NN
cord-104282-90t1m430	82	8	)	)	-RRB-
cord-104282-90t1m430	82	9	,	,	,
cord-104282-90t1m430	82	10	ALDH3	ALDH3	NNP
cord-104282-90t1m430	82	11	;	;	:
cord-104282-90t1m430	82	12	oligonucleotides	oligonucleotide	NNS
cord-104282-90t1m430	82	13	no	no	RB
cord-104282-90t1m430	82	14	.	.	.
cord-104282-90t1m430	83	1	13	13	CD
cord-104282-90t1m430	83	2	(	(	-LRB-
cord-104282-90t1m430	83	3	5	5	CD
cord-104282-90t1m430	83	4	'	'	``
cord-104282-90t1m430	83	5	AAACAGTICAAC	AAACAGTICAAC	NNP
cord-104282-90t1m430	83	6	-	-	HYPH
cord-104282-90t1m430	83	7	GGTACCAGC	GGTACCAGC	NNP
cord-104282-90t1m430	84	1	~TGCAGCTG	~TGCAGCTG	NFP
cord-104282-90t1m430	84	2	3	3	LS
cord-104282-90t1m430	84	3	'	'	''
cord-104282-90t1m430	84	4	)	)	-RRB-
cord-104282-90t1m430	84	5	and	and	CC
cord-104282-90t1m430	84	6	no	no	NN
cord-104282-90t1m430	84	7	.	.	NN
cord-104282-90t1m430	84	8	9	9	CD
cord-104282-90t1m430	84	9	(	(	-LRB-
cord-104282-90t1m430	84	10	pCDALDH	pCDALDH	NNP
cord-104282-90t1m430	84	11	as	as	IN
cord-104282-90t1m430	84	12	template	template	NN
cord-104282-90t1m430	84	13	)	)	-RRB-
cord-104282-90t1m430	84	14	,	,	,
cord-104282-90t1m430	84	15	ALDH4	ALDH4	NNP
cord-104282-90t1m430	84	16	;	;	:
cord-104282-90t1m430	84	17	oligonucleotides	oligonucleotide	NNS
cord-104282-90t1m430	84	18	no	no	RB
cord-104282-90t1m430	84	19	.	.	.
cord-104282-90t1m430	85	1	13	13	CD
cord-104282-90t1m430	85	2	and	and	CC
cord-104282-90t1m430	85	3	no	no	UH
cord-104282-90t1m430	85	4	.	.	.
cord-104282-90t1m430	86	1	9	9	LS
cord-104282-90t1m430	86	2	(	(	-LRB-
cord-104282-90t1m430	86	3	pCDALDHA481	pcdaldha481	VB
cord-104282-90t1m430	86	4	-	-	SYM
cord-104282-90t1m430	86	5	484	484	CD
cord-104282-90t1m430	86	6	as	as	IN
cord-104282-90t1m430	86	7	template	template	NN
cord-104282-90t1m430	86	8	)	)	-RRB-
cord-104282-90t1m430	86	9	,	,	,
cord-104282-90t1m430	86	10	ALDH5	ALDH5	NNP
cord-104282-90t1m430	86	11	;	;	:
cord-104282-90t1m430	86	12	oligonucleotides	oligonucleotide	NNS
cord-104282-90t1m430	86	13	no	no	RB
cord-104282-90t1m430	86	14	.	.	.
cord-104282-90t1m430	87	1	14	14	CD
cord-104282-90t1m430	87	2	(	(	-LRB-
cord-104282-90t1m430	87	3	5	5	CD
cord-104282-90t1m430	87	4	'	'	``
cord-104282-90t1m430	87	5	GAAATTCTTCGGTACC	GAAATTCTTCGGTACC	NNP
cord-104282-90t1m430	87	6	-	-	HYPH
cord-104282-90t1m430	87	7	AAACAGTTCAAC	AAACAGTTCAAC	NNP
cord-104282-90t1m430	87	8	3	3	CD
cord-104282-90t1m430	87	9	'	'	''
cord-104282-90t1m430	87	10	)	)	-RRB-
cord-104282-90t1m430	88	1	and	and	CC
cord-104282-90t1m430	88	2	no	no	UH
cord-104282-90t1m430	88	3	.	.	NN
cord-104282-90t1m430	88	4	9	9	CD
cord-104282-90t1m430	88	5	(	(	-LRB-
cord-104282-90t1m430	88	6	pCDALDH	pCDALDH	NNP
cord-104282-90t1m430	88	7	as	as	IN
cord-104282-90t1m430	88	8	template	template	NN
cord-104282-90t1m430	88	9	)	)	-RRB-
cord-104282-90t1m430	88	10	,	,	,
cord-104282-90t1m430	88	11	ALDH6	ALDH6	NNP
cord-104282-90t1m430	88	12	;	;	:
cord-104282-90t1m430	88	13	oligonucleotides	oligonucleotide	NNS
cord-104282-90t1m430	88	14	no	no	RB
cord-104282-90t1m430	88	15	.	.	.
cord-104282-90t1m430	89	1	14	14	CD
cord-104282-90t1m430	90	1	and	and	CC
cord-104282-90t1m430	90	2	no	no	UH
cord-104282-90t1m430	90	3	.	.	NN
cord-104282-90t1m430	90	4	9	9	CD
cord-104282-90t1m430	90	5	(	(	-LRB-
cord-104282-90t1m430	90	6	pCDALDHA481	pcdaldha481	VB
cord-104282-90t1m430	90	7	-	-	SYM
cord-104282-90t1m430	90	8	484	484	CD
cord-104282-90t1m430	90	9	as	as	IN
cord-104282-90t1m430	90	10	template	template	NN
cord-104282-90t1m430	90	11	)	)	-RRB-
cord-104282-90t1m430	90	12	,	,	.
cord-104282-90t1m430	91	1	ALDH7	aldh7	UH
cord-104282-90t1m430	91	2	;	;	:
cord-104282-90t1m430	91	3	oligonucleotides	oligonucleotide	NNS
cord-104282-90t1m430	91	4	no	no	RB
cord-104282-90t1m430	91	5	.	.	.
cord-104282-90t1m430	92	1	12	12	CD
cord-104282-90t1m430	92	2	and	and	CC
cord-104282-90t1m430	92	3	no	no	UH
cord-104282-90t1m430	92	4	.	.	.
cord-104282-90t1m430	93	1	9	9	LS
cord-104282-90t1m430	93	2	(	(	-LRB-
cord-104282-90t1m430	93	3	pCDALDHA457	pCDALDHA457	NNS
cord-104282-90t1m430	93	4	-	-	SYM
cord-104282-90t1m430	93	5	459	459	CD
cord-104282-90t1m430	93	6	as	as	IN
cord-104282-90t1m430	93	7	template	template	NN
cord-104282-90t1m430	93	8	)	)	-RRB-
cord-104282-90t1m430	93	9	,	,	,
cord-104282-90t1m430	93	10	and	and	CC
cord-104282-90t1m430	93	11	ALDH9	ALDH9	NNP
cord-104282-90t1m430	93	12	;	;	:
cord-104282-90t1m430	93	13	oligonucleotides	oligonucleotide	NNS
cord-104282-90t1m430	93	14	no	no	RB
cord-104282-90t1m430	93	15	.	.	.
cord-104282-90t1m430	94	1	12	12	CD
cord-104282-90t1m430	94	2	and	and	CC
cord-104282-90t1m430	94	3	no	no	NN
cord-104282-90t1m430	94	4	.	.	NN
cord-104282-90t1m430	94	5	9	9	CD
cord-104282-90t1m430	94	6	(	(	-LRB-
cord-104282-90t1m430	94	7	pCDALDHA460	pcdaldha460	CD
cord-104282-90t1m430	94	8	-	-	SYM
cord-104282-90t1m430	94	9	463	463	CD
cord-104282-90t1m430	94	10	as	as	IN
cord-104282-90t1m430	94	11	template	template	NN
cord-104282-90t1m430	94	12	)	)	-RRB-
cord-104282-90t1m430	94	13	.	.	.
cord-104282-90t1m430	95	1	These	these	DT
cord-104282-90t1m430	95	2	amplified	amplify	VBN
cord-104282-90t1m430	95	3	fragments	fragment	NNS
cord-104282-90t1m430	95	4	were	be	VBD
cord-104282-90t1m430	95	5	then	then	RB
cord-104282-90t1m430	95	6	digested	digest	VBN
cord-104282-90t1m430	95	7	with	with	IN
cord-104282-90t1m430	95	8	KpnI	KpnI	NNP
cord-104282-90t1m430	95	9	and	and	CC
cord-104282-90t1m430	95	10	HpaI	HpaI	NNP
cord-104282-90t1m430	95	11	and	and	CC
cord-104282-90t1m430	95	12	ligated	ligate	VBD
cord-104282-90t1m430	95	13	into	into	IN
cord-104282-90t1m430	95	14	the	the	DT
cord-104282-90t1m430	95	15	KpnI	KpnI	NNP
cord-104282-90t1m430	95	16	-	-	HYPH
cord-104282-90t1m430	95	17	EcoRV	EcoRV	NNP
cord-104282-90t1m430	95	18	site	site	NN
cord-104282-90t1m430	95	19	of	of	IN
cord-104282-90t1m430	95	20	pMIWCAT	pmiwcat	NN
cord-104282-90t1m430	95	21	.	.	.
cord-104282-90t1m430	96	1	The	the	DT
cord-104282-90t1m430	96	2	resultant	resultant	JJ
cord-104282-90t1m430	96	3	plasmids	plasmid	NNS
cord-104282-90t1m430	96	4	were	be	VBD
cord-104282-90t1m430	96	5	designated	designate	VBN
cord-104282-90t1m430	97	1	pMIWCAT	pMIWCAT	NNP
cord-104282-90t1m430	97	2	/	/	SYM
cord-104282-90t1m430	97	3	ALDH1	ALDH1	NNP
cord-104282-90t1m430	97	4	-	-	HYPH
cord-104282-90t1m430	97	5	7	7	CD
cord-104282-90t1m430	97	6	,	,	,
cord-104282-90t1m430	97	7	and	and	CC
cord-104282-90t1m430	98	1	pMIWCAT	pMIWCAT	NNP
cord-104282-90t1m430	98	2	/	/	SYM
cord-104282-90t1m430	98	3	ALDH9	ALDH9	NNP
cord-104282-90t1m430	98	4	,	,	,
cord-104282-90t1m430	98	5	respectively	respectively	RB
cord-104282-90t1m430	98	6	.	.	.
cord-104282-90t1m430	99	1	For	for	IN
cord-104282-90t1m430	99	2	construction	construction	NN
cord-104282-90t1m430	99	3	of	of	IN
cord-104282-90t1m430	99	4	pMIWCAT	pmiwcat	NN
cord-104282-90t1m430	99	5	/	/	SYM
cord-104282-90t1m430	99	6	ALDHS	ALDHS	NNP
cord-104282-90t1m430	99	7	,	,	,
cord-104282-90t1m430	99	8	pMIWCAT	pmiwcat	NN
cord-104282-90t1m430	99	9	/	/	SYM
cord-104282-90t1m430	99	10	ALDH2	ALDH2	NNP
cord-104282-90t1m430	99	11	,	,	,
cord-104282-90t1m430	99	12	which	which	WDT
cord-104282-90t1m430	99	13	has	have	VBZ
cord-104282-90t1m430	99	14	the	the	DT
cord-104282-90t1m430	99	15	unique	unique	JJ
cord-104282-90t1m430	99	16	PstI	PstI	NNP
cord-104282-90t1m430	99	17	site	site	NN
cord-104282-90t1m430	99	18	in	in	IN
cord-104282-90t1m430	99	19	the	the	DT
cord-104282-90t1m430	99	20	coding	code	VBG
cord-104282-90t1m430	99	21	sequence	sequence	NN
cord-104282-90t1m430	99	22	for	for	IN
cord-104282-90t1m430	99	23	the	the	DT
cord-104282-90t1m430	99	24	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	99	25	domain	domain	NN
cord-104282-90t1m430	99	26	of	of	IN
cord-104282-90t1m430	99	27	msALDH	msALDH	NNP
cord-104282-90t1m430	99	28	,	,	,
cord-104282-90t1m430	99	29	was	be	VBD
cord-104282-90t1m430	99	30	digested	digest	VBN
cord-104282-90t1m430	99	31	with	with	IN
cord-104282-90t1m430	99	32	PstI	PstI	NNP
cord-104282-90t1m430	99	33	and	and	CC
cord-104282-90t1m430	99	34	BamHI	BamHI	NNP
cord-104282-90t1m430	99	35	,	,	,
cord-104282-90t1m430	99	36	and	and	CC
cord-104282-90t1m430	99	37	ligated	ligate	VBD
cord-104282-90t1m430	99	38	into	into	IN
cord-104282-90t1m430	100	1	Pstl	Pstl	NNP
cord-104282-90t1m430	100	2	/	/	SYM
cord-104282-90t1m430	100	3	BamHI	BamHI	NNP
cord-104282-90t1m430	100	4	digested	digest	VBD
cord-104282-90t1m430	100	5	pMIWCATIALDH7	pmiwcatialdh7	PRP
cord-104282-90t1m430	100	6	.	.	.
cord-104282-90t1m430	101	1	Similarly	similarly	RB
cord-104282-90t1m430	101	2	,	,	,
cord-104282-90t1m430	101	3	the	the	DT
cord-104282-90t1m430	101	4	same	same	JJ
cord-104282-90t1m430	101	5	fragment	fragment	NN
cord-104282-90t1m430	101	6	was	be	VBD
cord-104282-90t1m430	101	7	ligated	ligate	VBN
cord-104282-90t1m430	101	8	into	into	IN
cord-104282-90t1m430	102	1	PstI	PstI	NNP
cord-104282-90t1m430	102	2	/	/	SYM
cord-104282-90t1m430	102	3	BamHI	BamHI	NNP
cord-104282-90t1m430	102	4	digested	digest	VBD
cord-104282-90t1m430	102	5	pM1WCAT	pM1WCAT	NNP
cord-104282-90t1m430	102	6	/	/	SYM
cord-104282-90t1m430	102	7	ALDH9	aldh9	NN
cord-104282-90t1m430	102	8	to	to	TO
cord-104282-90t1m430	102	9	construct	construct	VB
cord-104282-90t1m430	102	10	pMIWCAT	pmiwcat	NN
cord-104282-90t1m430	102	11	/	/	SYM
cord-104282-90t1m430	102	12	ALDH10	ALDH10	NNP
cord-104282-90t1m430	102	13	.	.	.
cord-104282-90t1m430	103	1	COS-1	COS-1	NNP
cord-104282-90t1m430	103	2	cells	cell	NNS
cord-104282-90t1m430	103	3	were	be	VBD
cord-104282-90t1m430	103	4	maintained	maintain	VBN
cord-104282-90t1m430	103	5	in	in	IN
cord-104282-90t1m430	103	6	DME	DME	NNP
cord-104282-90t1m430	103	7	with	with	IN
cord-104282-90t1m430	103	8	10	10	CD
cord-104282-90t1m430	103	9	%	%	NN
cord-104282-90t1m430	103	10	FBS	FBS	NNP
cord-104282-90t1m430	103	11	,	,	,
cord-104282-90t1m430	103	12	50	50	CD
cord-104282-90t1m430	103	13	U	u	NN
cord-104282-90t1m430	103	14	/	/	SYM
cord-104282-90t1m430	103	15	ml	ml	NN
cord-104282-90t1m430	103	16	penicillin	penicillin	NN
cord-104282-90t1m430	103	17	,	,	,
cord-104282-90t1m430	103	18	and	and	CC
cord-104282-90t1m430	103	19	50	50	CD
cord-104282-90t1m430	103	20	~g	~g	NN
cord-104282-90t1m430	103	21	/	/	SYM
cord-104282-90t1m430	103	22	ml	ml	NN
cord-104282-90t1m430	103	23	streptomycin	streptomycin	NNS
cord-104282-90t1m430	103	24	at	at	IN
cord-104282-90t1m430	103	25	37"C	37"c	CD
cord-104282-90t1m430	103	26	in	in	IN
cord-104282-90t1m430	103	27	a	a	DT
cord-104282-90t1m430	103	28	5	5	CD
cord-104282-90t1m430	103	29	%	%	NN
cord-104282-90t1m430	103	30	COz	COz	NNS
cord-104282-90t1m430	103	31	incubator	incubator	NN
cord-104282-90t1m430	103	32	,	,	,
cord-104282-90t1m430	103	33	and	and	CC
cord-104282-90t1m430	103	34	were	be	VBD
cord-104282-90t1m430	103	35	replated	replate	VBN
cord-104282-90t1m430	103	36	the	the	DT
cord-104282-90t1m430	103	37	day	day	NN
cord-104282-90t1m430	103	38	before	before	IN
cord-104282-90t1m430	103	39	transfection	transfection	NN
cord-104282-90t1m430	103	40	by	by	IN
cord-104282-90t1m430	103	41	trypsinization	trypsinization	NN
cord-104282-90t1m430	103	42	.	.	.
cord-104282-90t1m430	104	1	Transfection	Transfection	NNP
cord-104282-90t1m430	104	2	was	be	VBD
cord-104282-90t1m430	104	3	performed	perform	VBN
cord-104282-90t1m430	104	4	4	4	CD
cord-104282-90t1m430	104	5	h	h	NN
cord-104282-90t1m430	104	6	after	after	IN
cord-104282-90t1m430	104	7	the	the	DT
cord-104282-90t1m430	104	8	medium	medium	NN
cord-104282-90t1m430	104	9	was	be	VBD
cord-104282-90t1m430	104	10	replaced	replace	VBN
cord-104282-90t1m430	104	11	by	by	IN
cord-104282-90t1m430	104	12	the	the	DT
cord-104282-90t1m430	104	13	fresh	fresh	JJ
cord-104282-90t1m430	104	14	one	one	CD
cord-104282-90t1m430	104	15	.	.	.
cord-104282-90t1m430	105	1	For	for	IN
cord-104282-90t1m430	105	2	subeellniar	subeellniar	JJ
cord-104282-90t1m430	105	3	fractionation	fractionation	NN
cord-104282-90t1m430	105	4	experiments	experiment	NNS
cord-104282-90t1m430	105	5	,	,	,
cord-104282-90t1m430	105	6	cells	cell	NNS
cord-104282-90t1m430	105	7	plated	plate	VBN
cord-104282-90t1m430	105	8	in	in	IN
cord-104282-90t1m430	105	9	a	a	DT
cord-104282-90t1m430	105	10	lO0-mm	lO0-mm	NNP
cord-104282-90t1m430	105	11	dish	dish	NN
cord-104282-90t1m430	105	12	(	(	-LRB-
cord-104282-90t1m430	105	13	50	50	CD
cord-104282-90t1m430	105	14	-	-	SYM
cord-104282-90t1m430	105	15	70	70	CD
cord-104282-90t1m430	105	16	%	%	NN
cord-104282-90t1m430	105	17	confluem	confluem	NN
cord-104282-90t1m430	105	18	)	)	-RRB-
cord-104282-90t1m430	105	19	were	be	VBD
cord-104282-90t1m430	105	20	transfected	transfecte	VBN
cord-104282-90t1m430	105	21	with	with	IN
cord-104282-90t1m430	105	22	an	an	DT
cord-104282-90t1m430	105	23	expression	expression	NN
cord-104282-90t1m430	105	24	plasmid	plasmid	NN
cord-104282-90t1m430	105	25	(	(	-LRB-
cord-104282-90t1m430	105	26	20	20	CD
cord-104282-90t1m430	105	27	~g	~g	NNP
cord-104282-90t1m430	105	28	)	)	-RRB-
cord-104282-90t1m430	105	29	using	use	VBG
cord-104282-90t1m430	105	30	the	the	DT
cord-104282-90t1m430	105	31	cai-	cai-	NN
cord-104282-90t1m430	105	32	sequences	sequence	NNS
cord-104282-90t1m430	105	33	of	of	IN
cord-104282-90t1m430	105	34	the	the	DT
cord-104282-90t1m430	105	35	carboxyl	carboxyl	NN
cord-104282-90t1m430	105	36	termini	termini	NN
cord-104282-90t1m430	105	37	of	of	IN
cord-104282-90t1m430	105	38	wild	wild	JJ
cord-104282-90t1m430	105	39	-	-	HYPH
cord-104282-90t1m430	105	40	type	type	NN
cord-104282-90t1m430	105	41	and	and	CC
cord-104282-90t1m430	105	42	truncated	truncated	JJ
cord-104282-90t1m430	105	43	forms	form	NNS
cord-104282-90t1m430	105	44	of	of	IN
cord-104282-90t1m430	105	45	msALDH	msALDH	NNP
cord-104282-90t1m430	105	46	.	.	.
cord-104282-90t1m430	106	1	The	the	DT
cord-104282-90t1m430	106	2	single	single	JJ
cord-104282-90t1m430	106	3	amino	amino	NN
cord-104282-90t1m430	106	4	acid	acid	NN
cord-104282-90t1m430	106	5	code	code	NN
cord-104282-90t1m430	106	6	is	be	VBZ
cord-104282-90t1m430	106	7	used	use	VBN
cord-104282-90t1m430	106	8	,	,	,
cord-104282-90t1m430	106	9	and	and	CC
cord-104282-90t1m430	106	10	the	the	DT
cord-104282-90t1m430	106	11	amino	amino	NN
cord-104282-90t1m430	106	12	acid	acid	NN
cord-104282-90t1m430	106	13	numbers	number	NNS
cord-104282-90t1m430	106	14	of	of	IN
cord-104282-90t1m430	106	15	msALDH	msALDH	NNS
cord-104282-90t1m430	106	16	are	be	VBP
cord-104282-90t1m430	106	17	shown	show	VBN
cord-104282-90t1m430	106	18	at	at	IN
cord-104282-90t1m430	106	19	the	the	DT
cord-104282-90t1m430	106	20	top	top	NN
cord-104282-90t1m430	106	21	.	.	.
cord-104282-90t1m430	107	1	Positively	positively	RB
cord-104282-90t1m430	107	2	and	and	CC
cord-104282-90t1m430	107	3	negatively	negatively	RB
cord-104282-90t1m430	107	4	charged	charge	VBN
cord-104282-90t1m430	107	5	amino	amino	NN
cord-104282-90t1m430	107	6	acids	acid	NNS
cord-104282-90t1m430	107	7	are	be	VBP
cord-104282-90t1m430	107	8	marked	mark	VBN
cord-104282-90t1m430	107	9	+	+	SYM
cord-104282-90t1m430	107	10	and	and	CC
cord-104282-90t1m430	107	11	-at	-at	NFP
cord-104282-90t1m430	107	12	the	the	DT
cord-104282-90t1m430	107	13	bottom	bottom	NN
cord-104282-90t1m430	107	14	,	,	,
cord-104282-90t1m430	107	15	respectively	respectively	RB
cord-104282-90t1m430	107	16	.	.	.
cord-104282-90t1m430	108	1	The	the	DT
cord-104282-90t1m430	108	2	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	108	3	domains	domain	NNS
cord-104282-90t1m430	108	4	are	be	VBP
cord-104282-90t1m430	108	5	underlined	underline	VBN
cord-104282-90t1m430	108	6	.	.	.
cord-104282-90t1m430	109	1	The	the	DT
cord-104282-90t1m430	109	2	eDNA	edna	NN
cord-104282-90t1m430	109	3	for	for	IN
cord-104282-90t1m430	109	4	msALDH	msALDH	NNP
cord-104282-90t1m430	109	5	was	be	VBD
cord-104282-90t1m430	109	6	converted	convert	VBN
cord-104282-90t1m430	109	7	to	to	IN
cord-104282-90t1m430	109	8	code	code	NN
cord-104282-90t1m430	109	9	for	for	IN
cord-104282-90t1m430	109	10	three	three	CD
cord-104282-90t1m430	109	11	truncated	truncated	JJ
cord-104282-90t1m430	109	12	proteins	protein	NNS
cord-104282-90t1m430	109	13	,	,	,
cord-104282-90t1m430	109	14	msALDHA450	msALDHA450	NNP
cord-104282-90t1m430	109	15	-	-	HYPH
cord-104282-90t1m430	109	16	484	484	CD
cord-104282-90t1m430	109	17	,	,	,
cord-104282-90t1m430	109	18	msALDHA471	msaldha471	CD
cord-104282-90t1m430	109	19	-	-	SYM
cord-104282-90t1m430	109	20	484	484	CD
cord-104282-90t1m430	109	21	,	,	,
cord-104282-90t1m430	109	22	or	or	CC
cord-104282-90t1m430	109	23	ms	ms	NNP
cord-104282-90t1m430	109	24	-	-	NNP
cord-104282-90t1m430	109	25	ALDHA481	ALDHA481	NNP
cord-104282-90t1m430	109	26	-	-	HYPH
cord-104282-90t1m430	109	27	484	484	CD
cord-104282-90t1m430	109	28	by	by	IN
cord-104282-90t1m430	109	29	oligonucleotide	oligonucleotide	RB
cord-104282-90t1m430	109	30	-	-	HYPH
cord-104282-90t1m430	109	31	directed	direct	VBN
cord-104282-90t1m430	109	32	mutagenesis	mutagenesis	NN
cord-104282-90t1m430	109	33	.	.	.
cord-104282-90t1m430	110	1	cium	cium	NNP
cord-104282-90t1m430	110	2	phosphate	phosphate	NN
cord-104282-90t1m430	110	3	precipitation	precipitation	NN
cord-104282-90t1m430	110	4	method	method	NN
cord-104282-90t1m430	110	5	(	(	-LRB-
cord-104282-90t1m430	110	6	48	48	CD
cord-104282-90t1m430	110	7	)	)	-RRB-
cord-104282-90t1m430	110	8	.	.	.
cord-104282-90t1m430	111	1	For	for	IN
cord-104282-90t1m430	111	2	immunottuorescent	immunottuorescent	NN
cord-104282-90t1m430	111	3	experiments	experiment	NNS
cord-104282-90t1m430	111	4	,	,	,
cord-104282-90t1m430	111	5	cells	cell	NNS
cord-104282-90t1m430	111	6	were	be	VBD
cord-104282-90t1m430	111	7	grown	grow	VBN
cord-104282-90t1m430	111	8	on	on	IN
cord-104282-90t1m430	111	9	a	a	DT
cord-104282-90t1m430	111	10	22	22	CD
cord-104282-90t1m430	111	11	×	×	NN
cord-104282-90t1m430	111	12	22-ram	22-ram	CD
cord-104282-90t1m430	111	13	coverslip	coverslip	NN
cord-104282-90t1m430	111	14	in	in	IN
cord-104282-90t1m430	111	15	a	a	DT
cord-104282-90t1m430	111	16	35-ram	35-ram	CD
cord-104282-90t1m430	111	17	dish	dish	NN
cord-104282-90t1m430	111	18	(	(	-LRB-
cord-104282-90t1m430	111	19	10	10	CD
cord-104282-90t1m430	111	20	-	-	SYM
cord-104282-90t1m430	111	21	20	20	CD
cord-104282-90t1m430	111	22	%	%	NN
cord-104282-90t1m430	111	23	confluent	confluent	JJ
cord-104282-90t1m430	111	24	)	)	-RRB-
cord-104282-90t1m430	111	25	and	and	CC
cord-104282-90t1m430	111	26	were	be	VBD
cord-104282-90t1m430	111	27	transfected	transfecte	VBN
cord-104282-90t1m430	111	28	with	with	IN
cord-104282-90t1m430	111	29	4	4	CD
cord-104282-90t1m430	111	30	/~g	/~g	CD
cord-104282-90t1m430	111	31	plasmid	plasmid	NN
cord-104282-90t1m430	111	32	DNA	dna	NN
cord-104282-90t1m430	111	33	per	per	IN
cord-104282-90t1m430	111	34	35-mm	35-mm	CD
cord-104282-90t1m430	111	35	dish	dish	NN
cord-104282-90t1m430	111	36	.	.	.
cord-104282-90t1m430	112	1	For	for	IN
cord-104282-90t1m430	112	2	immunogold	immunogold	JJ
cord-104282-90t1m430	112	3	localization	localization	NN
cord-104282-90t1m430	112	4	of	of	IN
cord-104282-90t1m430	112	5	CAT	CAT	NNP
cord-104282-90t1m430	112	6	/	/	SYM
cord-104282-90t1m430	112	7	ALDH	ALDH	NNP
cord-104282-90t1m430	112	8	chimeras	chimera	NNS
cord-104282-90t1m430	112	9	,	,	,
cord-104282-90t1m430	112	10	cells	cell	NNS
cord-104282-90t1m430	112	11	plated	plate	VBN
cord-104282-90t1m430	112	12	in	in	IN
cord-104282-90t1m430	112	13	a	a	DT
cord-104282-90t1m430	112	14	60-ram	60-ram	CD
cord-104282-90t1m430	112	15	dish	dish	NN
cord-104282-90t1m430	112	16	(	(	-LRB-
cord-104282-90t1m430	112	17	50	50	CD
cord-104282-90t1m430	112	18	-	-	SYM
cord-104282-90t1m430	112	19	70	70	CD
cord-104282-90t1m430	112	20	%	%	NN
cord-104282-90t1m430	112	21	confluent	confluent	JJ
cord-104282-90t1m430	112	22	)	)	-RRB-
cord-104282-90t1m430	112	23	were	be	VBD
cord-104282-90t1m430	112	24	transfected	transfecte	VBN
cord-104282-90t1m430	112	25	with	with	IN
cord-104282-90t1m430	112	26	10	10	CD
cord-104282-90t1m430	112	27	ttg	ttg	NNP
cord-104282-90t1m430	112	28	plasmid	plasmid	NN
cord-104282-90t1m430	112	29	DNA	dna	NN
cord-104282-90t1m430	112	30	per	per	IN
cord-104282-90t1m430	112	31	dish	dish	NN
cord-104282-90t1m430	112	32	.	.	.
cord-104282-90t1m430	113	1	4	4	LS
cord-104282-90t1m430	113	2	h	h	NN
cord-104282-90t1m430	113	3	after	after	IN
cord-104282-90t1m430	113	4	application	application	NN
cord-104282-90t1m430	113	5	of	of	IN
cord-104282-90t1m430	113	6	DNA	dna	NN
cord-104282-90t1m430	113	7	-	-	HYPH
cord-104282-90t1m430	113	8	calcium	calcium	NN
cord-104282-90t1m430	113	9	phosphate	phosphate	NN
cord-104282-90t1m430	113	10	precipitate	precipitate	NN
cord-104282-90t1m430	113	11	at	at	IN
cord-104282-90t1m430	113	12	37	37	CD
cord-104282-90t1m430	113	13	°	°	NN
cord-104282-90t1m430	113	14	C	c	NN
cord-104282-90t1m430	113	15	,	,	,
cord-104282-90t1m430	113	16	cells	cell	NNS
cord-104282-90t1m430	113	17	were	be	VBD
cord-104282-90t1m430	113	18	shocked	shock	VBN
cord-104282-90t1m430	113	19	with	with	IN
cord-104282-90t1m430	113	20	15	15	CD
cord-104282-90t1m430	113	21	%	%	NN
cord-104282-90t1m430	113	22	glycerol	glycerol	NN
cord-104282-90t1m430	113	23	for	for	IN
cord-104282-90t1m430	113	24	2	2	CD
cord-104282-90t1m430	113	25	rain	rain	NN
cord-104282-90t1m430	113	26	at	at	IN
cord-104282-90t1m430	113	27	room	room	NN
cord-104282-90t1m430	113	28	temperature	temperature	NN
cord-104282-90t1m430	113	29	,	,	,
cord-104282-90t1m430	113	30	then	then	RB
cord-104282-90t1m430	113	31	incubated	incubate	VBN
cord-104282-90t1m430	113	32	again	again	RB
cord-104282-90t1m430	113	33	at	at	IN
cord-104282-90t1m430	113	34	370C	370c	CD
cord-104282-90t1m430	113	35	for	for	IN
cord-104282-90t1m430	113	36	an	an	DT
cord-104282-90t1m430	113	37	additional	additional	JJ
cord-104282-90t1m430	113	38	44	44	CD
cord-104282-90t1m430	113	39	h	h	NN
cord-104282-90t1m430	113	40	before	before	IN
cord-104282-90t1m430	113	41	harvesting	harvest	VBG
cord-104282-90t1m430	113	42	for	for	IN
cord-104282-90t1m430	113	43	subcellular	subcellular	JJ
cord-104282-90t1m430	113	44	fractionation	fractionation	NN
cord-104282-90t1m430	113	45	or	or	CC
cord-104282-90t1m430	113	46	fixation	fixation	NN
cord-104282-90t1m430	113	47	for	for	IN
cord-104282-90t1m430	113	48	indirect	indirect	JJ
cord-104282-90t1m430	113	49	immunofluorescence	immunofluorescence	NN
cord-104282-90t1m430	113	50	microscopy	microscopy	NN
cord-104282-90t1m430	113	51	or	or	CC
cord-104282-90t1m430	113	52	immunoelectmn	immunoelectmn	NN
cord-104282-90t1m430	113	53	microscopy	microscopy	NN
cord-104282-90t1m430	113	54	.	.	.
cord-104282-90t1m430	114	1	Cell	cell	NN
cord-104282-90t1m430	114	2	fractionation	fractionation	NN
cord-104282-90t1m430	114	3	was	be	VBD
cord-104282-90t1m430	114	4	performed	perform	VBN
cord-104282-90t1m430	114	5	essentially	essentially	RB
cord-104282-90t1m430	114	6	as	as	IN
cord-104282-90t1m430	114	7	described	describe	VBN
cord-104282-90t1m430	114	8	previously	previously	RB
cord-104282-90t1m430	114	9	for	for	IN
cord-104282-90t1m430	114	10	COS	COS	NNP
cord-104282-90t1m430	114	11	-	-	:
cord-104282-90t1m430	114	12	I	-PRON-	PRP
cord-104282-90t1m430	114	13	cells	cell	NNS
cord-104282-90t1m430	114	14	by	by	IN
cord-104282-90t1m430	114	15	Clark	Clark	NNP
cord-104282-90t1m430	114	16	and	and	CC
cord-104282-90t1m430	114	17	Waterman	Waterman	NNP
cord-104282-90t1m430	114	18	(	(	-LRB-
cord-104282-90t1m430	114	19	10	10	CD
cord-104282-90t1m430	114	20	)	)	-RRB-
cord-104282-90t1m430	114	21	with	with	IN
cord-104282-90t1m430	114	22	slight	slight	JJ
cord-104282-90t1m430	114	23	modifications	modification	NNS
cord-104282-90t1m430	114	24	.	.	.
cord-104282-90t1m430	115	1	Briefly	briefly	RB
cord-104282-90t1m430	115	2	,	,	,
cord-104282-90t1m430	115	3	cells	cell	NNS
cord-104282-90t1m430	115	4	were	be	VBD
cord-104282-90t1m430	115	5	washed	wash	VBN
cord-104282-90t1m430	115	6	once	once	RB
cord-104282-90t1m430	115	7	with	with	IN
cord-104282-90t1m430	115	8	PBS	PBS	NNP
cord-104282-90t1m430	115	9	and	and	CC
cord-104282-90t1m430	115	10	harvested	harvest	VBN
cord-104282-90t1m430	115	11	in	in	IN
cord-104282-90t1m430	115	12	5	5	CD
cord-104282-90t1m430	115	13	ml	ml	NNS
cord-104282-90t1m430	115	14	of	of	IN
cord-104282-90t1m430	115	15	ice	ice	NN
cord-104282-90t1m430	115	16	-	-	HYPH
cord-104282-90t1m430	115	17	cold	cold	JJ
cord-104282-90t1m430	115	18	0.5	0.5	CD
cord-104282-90t1m430	115	19	M	m	NN
cord-104282-90t1m430	115	20	sucrose	sucrose	NN
cord-104282-90t1m430	115	21	containing	contain	VBG
cord-104282-90t1m430	115	22	10	10	CD
cord-104282-90t1m430	115	23	mM	mm	CD
cord-104282-90t1m430	115	24	Tris	Tris	NNP
cord-104282-90t1m430	115	25	-	-	HYPH
cord-104282-90t1m430	115	26	HCl	HCl	NNP
cord-104282-90t1m430	115	27	,	,	,
cord-104282-90t1m430	115	28	pH	pH	NNP
cord-104282-90t1m430	115	29	7.4	7.4	CD
cord-104282-90t1m430	115	30	,	,	,
cord-104282-90t1m430	115	31	1	1	CD
cord-104282-90t1m430	115	32	mM	mm	CD
cord-104282-90t1m430	115	33	EDTA	EDTA	NNP
cord-104282-90t1m430	115	34	,	,	,
cord-104282-90t1m430	115	35	10	10	CD
cord-104282-90t1m430	115	36	#	#	$
cord-104282-90t1m430	115	37	g	g	NN
cord-104282-90t1m430	115	38	/	/	SYM
cord-104282-90t1m430	115	39	ml	ml	NN
cord-104282-90t1m430	115	40	leupeptin	leupeptin	NN
cord-104282-90t1m430	115	41	,	,	,
cord-104282-90t1m430	115	42	10/~g	10/~g	NNP
cord-104282-90t1m430	115	43	/	/	SYM
cord-104282-90t1m430	115	44	ml	ml	NNS
cord-104282-90t1m430	115	45	pepstatin	pepstatin	NNP
cord-104282-90t1m430	115	46	A	A	NNP
cord-104282-90t1m430	115	47	,	,	,
cord-104282-90t1m430	115	48	0.5	0.5	CD
cord-104282-90t1m430	115	49	m_M	m_m	JJ
cord-104282-90t1m430	115	50	PMSF	PMSF	NNP
cord-104282-90t1m430	115	51	,	,	,
cord-104282-90t1m430	115	52	and	and	CC
cord-104282-90t1m430	115	53	10	10	CD
cord-104282-90t1m430	115	54	U	u	NN
cord-104282-90t1m430	115	55	/	/	SYM
cord-104282-90t1m430	115	56	ml	ml	NNP
cord-104282-90t1m430	115	57	Trasyol	Trasyol	NNP
cord-104282-90t1m430	115	58	(	(	-LRB-
cord-104282-90t1m430	115	59	0.5	0.5	CD
cord-104282-90t1m430	115	60	M	M	NNP
cord-104282-90t1m430	115	61	STE	STE	NNP
cord-104282-90t1m430	115	62	)	)	-RRB-
cord-104282-90t1m430	115	63	.	.	.
cord-104282-90t1m430	116	1	After	after	IN
cord-104282-90t1m430	116	2	centrifugation	centrifugation	NN
cord-104282-90t1m430	116	3	at	at	IN
cord-104282-90t1m430	116	4	800	800	CD
cord-104282-90t1m430	116	5	g	g	NN
cord-104282-90t1m430	116	6	for	for	IN
cord-104282-90t1m430	116	7	5	5	CD
cord-104282-90t1m430	116	8	rain	rain	NN
cord-104282-90t1m430	116	9	,	,	,
cord-104282-90t1m430	116	10	the	the	DT
cord-104282-90t1m430	116	11	pellet	pellet	NN
cord-104282-90t1m430	116	12	was	be	VBD
cord-104282-90t1m430	116	13	suspended	suspend	VBN
cord-104282-90t1m430	116	14	in	in	IN
cord-104282-90t1m430	116	15	0.5	0.5	CD
cord-104282-90t1m430	116	16	ml	ml	NNS
cord-104282-90t1m430	116	17	of	of	IN
cord-104282-90t1m430	116	18	0.5	0.5	CD
cord-104282-90t1m430	116	19	M	M	NNP
cord-104282-90t1m430	116	20	STE	STE	NNP
cord-104282-90t1m430	116	21	,	,	,
cord-104282-90t1m430	116	22	homogenized	homogenize	VBN
cord-104282-90t1m430	116	23	with	with	IN
cord-104282-90t1m430	116	24	a	a	DT
cord-104282-90t1m430	116	25	Teflon	Teflon	NNP
cord-104282-90t1m430	116	26	-	-	HYPH
cord-104282-90t1m430	116	27	glass	glass	NN
cord-104282-90t1m430	116	28	homogenizer	homogenizer	NN
cord-104282-90t1m430	116	29	,	,	,
cord-104282-90t1m430	116	30	then	then	RB
cord-104282-90t1m430	116	31	diluted	dilute	VBN
cord-104282-90t1m430	116	32	with	with	IN
cord-104282-90t1m430	116	33	an	an	DT
cord-104282-90t1m430	116	34	equal	equal	JJ
cord-104282-90t1m430	116	35	volume	volume	NN
cord-104282-90t1m430	116	36	of	of	IN
cord-104282-90t1m430	116	37	10	10	CD
cord-104282-90t1m430	116	38	mM	mM	NNP
cord-104282-90t1m430	116	39	Tris	Tris	NNP
cord-104282-90t1m430	116	40	-	-	HYPH
cord-104282-90t1m430	116	41	HCl	HCl	NNP
cord-104282-90t1m430	116	42	,	,	,
cord-104282-90t1m430	116	43	pH	pH	NNP
cord-104282-90t1m430	116	44	7.4	7.4	CD
cord-104282-90t1m430	116	45	,	,	,
cord-104282-90t1m430	116	46	1	1	CD
cord-104282-90t1m430	116	47	mM	mm	CD
cord-104282-90t1m430	116	48	EDTA	EDTA	NNP
cord-104282-90t1m430	116	49	,	,	,
cord-104282-90t1m430	116	50	10	10	CD
cord-104282-90t1m430	116	51	/	/	SYM
cord-104282-90t1m430	116	52	tg	tg	NNP
cord-104282-90t1m430	116	53	/	/	SYM
cord-104282-90t1m430	116	54	nd	nd	NNP
cord-104282-90t1m430	116	55	leupeptin	leupeptin	NNP
cord-104282-90t1m430	116	56	,	,	,
cord-104282-90t1m430	117	1	10/=g	10/=g	CD
cord-104282-90t1m430	117	2	/	/	SYM
cord-104282-90t1m430	117	3	ml	ml	NNS
cord-104282-90t1m430	117	4	pepstatin	pepstatin	NNP
cord-104282-90t1m430	117	5	A	A	NNP
cord-104282-90t1m430	117	6	,	,	,
cord-104282-90t1m430	117	7	0.5	0.5	CD
cord-104282-90t1m430	117	8	mM	mm	CD
cord-104282-90t1m430	117	9	PMSF	PMSF	NNP
cord-104282-90t1m430	117	10	,	,	,
cord-104282-90t1m430	117	11	and	and	CC
cord-104282-90t1m430	117	12	10	10	CD
cord-104282-90t1m430	117	13	U	u	NN
cord-104282-90t1m430	117	14	/	/	SYM
cord-104282-90t1m430	117	15	rni	rni	NNP
cord-104282-90t1m430	117	16	Trasyol	Trasyol	NNP
cord-104282-90t1m430	117	17	to	to	TO
cord-104282-90t1m430	117	18	obtain	obtain	VB
cord-104282-90t1m430	117	19	isotonic	isotonic	JJ
cord-104282-90t1m430	117	20	conditions	condition	NNS
cord-104282-90t1m430	117	21	.	.	.
cord-104282-90t1m430	118	1	The	the	DT
cord-104282-90t1m430	118	2	total	total	JJ
cord-104282-90t1m430	118	3	homogenate	homogenate	NNP
cord-104282-90t1m430	118	4	(	(	-LRB-
cord-104282-90t1m430	118	5	designated	designate	VBN
cord-104282-90t1m430	118	6	H	H	NNP
cord-104282-90t1m430	118	7	)	)	-RRB-
cord-104282-90t1m430	118	8	was	be	VBD
cord-104282-90t1m430	118	9	layered	layer	VBN
cord-104282-90t1m430	118	10	over	over	IN
cord-104282-90t1m430	118	11	0.5	0.5	CD
cord-104282-90t1m430	118	12	ml	ml	NNS
cord-104282-90t1m430	118	13	of	of	IN
cord-104282-90t1m430	118	14	0.5	0.5	CD
cord-104282-90t1m430	118	15	M	M	NNP
cord-104282-90t1m430	118	16	STE	STE	NNP
cord-104282-90t1m430	118	17	and	and	CC
cord-104282-90t1m430	118	18	centrifuged	centrifuge	VBD
cord-104282-90t1m430	118	19	at	at	IN
cord-104282-90t1m430	118	20	800	800	CD
cord-104282-90t1m430	118	21	g	g	NN
cord-104282-90t1m430	118	22	for	for	IN
cord-104282-90t1m430	118	23	10	10	CD
cord-104282-90t1m430	118	24	rain	rain	NN
cord-104282-90t1m430	118	25	at	at	IN
cord-104282-90t1m430	118	26	4	4	CD
cord-104282-90t1m430	118	27	°	°	NN
cord-104282-90t1m430	118	28	C	c	NN
cord-104282-90t1m430	118	29	using	use	VBG
cord-104282-90t1m430	118	30	a	a	DT
cord-104282-90t1m430	118	31	swing	swing	NN
cord-104282-90t1m430	118	32	but	but	CC
cord-104282-90t1m430	118	33	bucket	bucket	NN
cord-104282-90t1m430	118	34	,	,	,
cord-104282-90t1m430	118	35	yielding	yield	VBG
cord-104282-90t1m430	118	36	a	a	DT
cord-104282-90t1m430	118	37	pellet	pellet	NN
cord-104282-90t1m430	118	38	(	(	-LRB-
cord-104282-90t1m430	118	39	P1	p1	NN
cord-104282-90t1m430	118	40	)	)	-RRB-
cord-104282-90t1m430	118	41	consisting	consist	VBG
cord-104282-90t1m430	118	42	mainly	mainly	RB
cord-104282-90t1m430	118	43	of	of	IN
cord-104282-90t1m430	118	44	nuclei	nucleus	NNS
cord-104282-90t1m430	118	45	and	and	CC
cord-104282-90t1m430	118	46	unbroken	unbroken	JJ
cord-104282-90t1m430	118	47	cells	cell	NNS
cord-104282-90t1m430	118	48	.	.	.
cord-104282-90t1m430	119	1	The	the	DT
cord-104282-90t1m430	119	2	supernatant	supernatant	JJ
cord-104282-90t1m430	119	3	and	and	CC
cord-104282-90t1m430	119	4	the	the	DT
cord-104282-90t1m430	119	5	interface	interface	NN
cord-104282-90t1m430	119	6	were	be	VBD
cord-104282-90t1m430	119	7	again	again	RB
cord-104282-90t1m430	119	8	layered	layer	VBN
cord-104282-90t1m430	119	9	over	over	IN
cord-104282-90t1m430	119	10	0.5	0.5	CD
cord-104282-90t1m430	119	11	M	M	NNP
cord-104282-90t1m430	119	12	STE	STE	NNP
cord-104282-90t1m430	119	13	and	and	CC
cord-104282-90t1m430	119	14	centrifuged	centrifuge	VBD
cord-104282-90t1m430	119	15	as	as	IN
cord-104282-90t1m430	119	16	above	above	RB
cord-104282-90t1m430	119	17	at	at	IN
cord-104282-90t1m430	119	18	9,000	9,000	CD
cord-104282-90t1m430	119	19	g	g	NN
cord-104282-90t1m430	119	20	for	for	IN
cord-104282-90t1m430	119	21	10	10	CD
cord-104282-90t1m430	119	22	min	min	NN
cord-104282-90t1m430	119	23	to	to	TO
cord-104282-90t1m430	119	24	isolate	isolate	VB
cord-104282-90t1m430	119	25	mitochondrial	mitochondrial	JJ
cord-104282-90t1m430	119	26	fraction	fraction	NN
cord-104282-90t1m430	119	27	(	(	-LRB-
cord-104282-90t1m430	119	28	P2	P2	NNP
cord-104282-90t1m430	119	29	)	)	-RRB-
cord-104282-90t1m430	119	30	.	.	.
cord-104282-90t1m430	120	1	The	the	DT
cord-104282-90t1m430	120	2	resultant	resultant	JJ
cord-104282-90t1m430	120	3	supernatant	supernatant	NN
cord-104282-90t1m430	120	4	was	be	VBD
cord-104282-90t1m430	120	5	centrifuged	centrifuge	VBN
cord-104282-90t1m430	120	6	at	at	IN
cord-104282-90t1m430	120	7	88,000	88,000	CD
cord-104282-90t1m430	120	8	g	g	NN
cord-104282-90t1m430	120	9	for	for	IN
cord-104282-90t1m430	120	10	80	80	CD
cord-104282-90t1m430	120	11	rain	rain	NN
cord-104282-90t1m430	120	12	at	at	IN
cord-104282-90t1m430	120	13	40C	40c	CD
cord-104282-90t1m430	120	14	to	to	IN
cord-104282-90t1m430	120	15	sediment	sediment	NN
cord-104282-90t1m430	120	16	microsomal	microsomal	NNP
cord-104282-90t1m430	120	17	fraction	fraction	NN
cord-104282-90t1m430	120	18	(	(	-LRB-
cord-104282-90t1m430	120	19	P3	P3	NNP
cord-104282-90t1m430	120	20	,	,	,
cord-104282-90t1m430	120	21	mostly	mostly	RB
cord-104282-90t1m430	120	22	of	of	IN
cord-104282-90t1m430	120	23	the	the	DT
cord-104282-90t1m430	120	24	ER	ER	NNP
cord-104282-90t1m430	120	25	membrane	membrane	NN
cord-104282-90t1m430	120	26	)	)	-RRB-
cord-104282-90t1m430	120	27	.	.	.
cord-104282-90t1m430	121	1	The	the	DT
cord-104282-90t1m430	121	2	final	final	JJ
cord-104282-90t1m430	121	3	supernatant	supernatant	NN
cord-104282-90t1m430	121	4	,	,	,
cord-104282-90t1m430	121	5	consisting	consist	VBG
cord-104282-90t1m430	121	6	mostly	mostly	RB
cord-104282-90t1m430	121	7	of	of	IN
cord-104282-90t1m430	121	8	cytosol	cytosol	NN
cord-104282-90t1m430	121	9	,	,	,
cord-104282-90t1m430	121	10	was	be	VBD
cord-104282-90t1m430	121	11	designated	designate	VBN
cord-104282-90t1m430	121	12	$	$	$
cord-104282-90t1m430	121	13	3	3	CD
cord-104282-90t1m430	121	14	.	.	.
cord-104282-90t1m430	122	1	Membrane	membrane	NN
cord-104282-90t1m430	122	2	fractions	fraction	NNS
cord-104282-90t1m430	122	3	,	,	,
cord-104282-90t1m430	122	4	P1	P1	NNP
cord-104282-90t1m430	122	5	,	,	,
cord-104282-90t1m430	122	6	P2	P2	NNP
cord-104282-90t1m430	122	7	,	,	,
cord-104282-90t1m430	122	8	and	and	CC
cord-104282-90t1m430	122	9	P3	P3	NNP
cord-104282-90t1m430	122	10	were	be	VBD
cord-104282-90t1m430	122	11	resuspended	resuspend	VBN
cord-104282-90t1m430	122	12	by	by	IN
cord-104282-90t1m430	122	13	hand	hand	NN
cord-104282-90t1m430	122	14	homogenization	homogenization	NN
cord-104282-90t1m430	122	15	in	in	IN
cord-104282-90t1m430	122	16	0.25	0.25	CD
cord-104282-90t1m430	122	17	M	M	NNP
cord-104282-90t1m430	122	18	STE	STE	NNP
cord-104282-90t1m430	122	19	.	.	.
cord-104282-90t1m430	123	1	Endogenous	endogenous	JJ
cord-104282-90t1m430	123	2	enzyme	enzyme	NN
cord-104282-90t1m430	123	3	activities	activity	NNS
cord-104282-90t1m430	123	4	of	of	IN
cord-104282-90t1m430	123	5	FP2	fp2	NN
cord-104282-90t1m430	123	6	(	(	-LRB-
cord-104282-90t1m430	123	7	an	an	DT
cord-104282-90t1m430	123	8	ER	ER	NNP
cord-104282-90t1m430	123	9	marker	marker	NN
cord-104282-90t1m430	123	10	)	)	-RRB-
cord-104282-90t1m430	123	11	and	and	CC
cord-104282-90t1m430	123	12	succinate	succinate	NN
cord-104282-90t1m430	123	13	-	-	HYPH
cord-104282-90t1m430	123	14	cytochrome	cytochrome	JJ
cord-104282-90t1m430	123	15	c	c	NN
cord-104282-90t1m430	123	16	reductase	reductase	NN
cord-104282-90t1m430	123	17	(	(	-LRB-
cord-104282-90t1m430	123	18	a	a	DT
cord-104282-90t1m430	123	19	mitochondrial	mitochondrial	JJ
cord-104282-90t1m430	123	20	marker	marker	NN
cord-104282-90t1m430	123	21	)	)	-RRB-
cord-104282-90t1m430	123	22	were	be	VBD
cord-104282-90t1m430	123	23	assayed	assay	VBN
cord-104282-90t1m430	123	24	by	by	IN
cord-104282-90t1m430	123	25	the	the	DT
cord-104282-90t1m430	123	26	methods	method	NNS
cord-104282-90t1m430	123	27	of	of	IN
cord-104282-90t1m430	123	28	Omura	Omura	NNP
cord-104282-90t1m430	123	29	and	and	CC
cord-104282-90t1m430	123	30	Takesue	Takesue	NNP
cord-104282-90t1m430	123	31	(	(	-LRB-
cord-104282-90t1m430	123	32	31	31	CD
cord-104282-90t1m430	123	33	)	)	-RRB-
cord-104282-90t1m430	123	34	and	and	CC
cord-104282-90t1m430	123	35	King	King	NNP
cord-104282-90t1m430	123	36	(	(	-LRB-
cord-104282-90t1m430	123	37	17	17	CD
cord-104282-90t1m430	123	38	)	)	-RRB-
cord-104282-90t1m430	123	39	,	,	,
cord-104282-90t1m430	123	40	respectively	respectively	RB
cord-104282-90t1m430	123	41	.	.	.
cord-104282-90t1m430	124	1	Protein	protein	NN
cord-104282-90t1m430	124	2	was	be	VBD
cord-104282-90t1m430	124	3	measured	measure	VBN
cord-104282-90t1m430	124	4	by	by	IN
cord-104282-90t1m430	124	5	Bradford	Bradford	NNP
cord-104282-90t1m430	124	6	's	's	POS
cord-104282-90t1m430	124	7	method	method	NN
cord-104282-90t1m430	124	8	(	(	-LRB-
cord-104282-90t1m430	124	9	7	7	CD
cord-104282-90t1m430	124	10	)	)	-RRB-
cord-104282-90t1m430	124	11	.	.	.
cord-104282-90t1m430	125	1	Membrane	membrane	NN
cord-104282-90t1m430	125	2	fractions	fraction	NNS
cord-104282-90t1m430	125	3	(	(	-LRB-
cord-104282-90t1m430	125	4	100	100	CD
cord-104282-90t1m430	125	5	/	/	SYM
cord-104282-90t1m430	125	6	tg	tg	NNP
cord-104282-90t1m430	125	7	protein	protein	NNP
cord-104282-90t1m430	125	8	)	)	-RRB-
cord-104282-90t1m430	125	9	were	be	VBD
cord-104282-90t1m430	125	10	resuspended	resuspend	VBN
cord-104282-90t1m430	125	11	in	in	IN
cord-104282-90t1m430	125	12	0.8	0.8	CD
cord-104282-90t1m430	125	13	ml	ml	NNS
cord-104282-90t1m430	125	14	of	of	IN
cord-104282-90t1m430	125	15	100	100	CD
cord-104282-90t1m430	125	16	mM	mm	CD
cord-104282-90t1m430	125	17	Na2CO3	Na2CO3	NNP
cord-104282-90t1m430	125	18	(	(	-LRB-
cord-104282-90t1m430	125	19	pH	pH	NNP
cord-104282-90t1m430	125	20	11.5	11.5	CD
cord-104282-90t1m430	125	21	)	)	-RRB-
cord-104282-90t1m430	125	22	,	,	,
cord-104282-90t1m430	125	23	and	and	CC
cord-104282-90t1m430	125	24	were	be	VBD
cord-104282-90t1m430	125	25	incubated	incubate	VBN
cord-104282-90t1m430	125	26	for	for	IN
cord-104282-90t1m430	125	27	30	30	CD
cord-104282-90t1m430	125	28	rain	rain	NN
cord-104282-90t1m430	125	29	at	at	IN
cord-104282-90t1m430	125	30	0*C	0*c	CD
cord-104282-90t1m430	125	31	(	(	-LRB-
cord-104282-90t1m430	125	32	13	13	CD
cord-104282-90t1m430	125	33	)	)	-RRB-
cord-104282-90t1m430	125	34	.	.	.
cord-104282-90t1m430	126	1	The	the	DT
cord-104282-90t1m430	126	2	suspension	suspension	NN
cord-104282-90t1m430	126	3	was	be	VBD
cord-104282-90t1m430	126	4	then	then	RB
cord-104282-90t1m430	126	5	centrifuged	centrifuge	VBN
cord-104282-90t1m430	126	6	at	at	IN
cord-104282-90t1m430	126	7	88,000	88,000	CD
cord-104282-90t1m430	126	8	g	g	NN
cord-104282-90t1m430	126	9	for	for	IN
cord-104282-90t1m430	126	10	80	80	CD
cord-104282-90t1m430	126	11	rain	rain	NN
cord-104282-90t1m430	126	12	at	at	IN
cord-104282-90t1m430	126	13	40C	40c	CD
cord-104282-90t1m430	126	14	,	,	,
cord-104282-90t1m430	126	15	and	and	CC
cord-104282-90t1m430	126	16	the	the	DT
cord-104282-90t1m430	126	17	pellet	pellet	NN
cord-104282-90t1m430	126	18	(	(	-LRB-
cord-104282-90t1m430	126	19	P	p	NN
cord-104282-90t1m430	126	20	)	)	-RRB-
cord-104282-90t1m430	126	21	was	be	VBD
cord-104282-90t1m430	126	22	suspended	suspend	VBN
cord-104282-90t1m430	126	23	in	in	IN
cord-104282-90t1m430	126	24	100/~i	100/~i	CD
cord-104282-90t1m430	126	25	of	of	IN
cord-104282-90t1m430	126	26	SDS	SDS	NNP
cord-104282-90t1m430	126	27	-	-	HYPH
cord-104282-90t1m430	126	28	PAGE	page	NN
cord-104282-90t1m430	126	29	sample	sample	NN
cord-104282-90t1m430	126	30	buffer	buffer	NN
cord-104282-90t1m430	126	31	(	(	-LRB-
cord-104282-90t1m430	126	32	20	20	CD
cord-104282-90t1m430	126	33	)	)	-RRB-
cord-104282-90t1m430	126	34	.	.	.
cord-104282-90t1m430	127	1	The	the	DT
cord-104282-90t1m430	127	2	supernatant	supernatant	NN
cord-104282-90t1m430	127	3	(	(	-LRB-
cord-104282-90t1m430	127	4	S	s	NN
cord-104282-90t1m430	127	5	)	)	-RRB-
cord-104282-90t1m430	127	6	was	be	VBD
cord-104282-90t1m430	127	7	precipitated	precipitate	VBN
cord-104282-90t1m430	127	8	with	with	IN
cord-104282-90t1m430	127	9	10	10	CD
cord-104282-90t1m430	127	10	%	%	NN
cord-104282-90t1m430	127	11	TCA	TCA	NNP
cord-104282-90t1m430	127	12	,	,	,
cord-104282-90t1m430	127	13	washed	wash	VBD
cord-104282-90t1m430	127	14	twice	twice	RB
cord-104282-90t1m430	127	15	with	with	IN
cord-104282-90t1m430	127	16	90	90	CD
cord-104282-90t1m430	127	17	%	%	NN
cord-104282-90t1m430	127	18	ethanol	ethanol	NN
cord-104282-90t1m430	127	19	and	and	CC
cord-104282-90t1m430	127	20	once	once	RB
cord-104282-90t1m430	127	21	with	with	IN
cord-104282-90t1m430	127	22	diethyl	diethyl	JJ
cord-104282-90t1m430	127	23	ether	ether	NN
cord-104282-90t1m430	127	24	,	,	,
cord-104282-90t1m430	127	25	and	and	CC
cord-104282-90t1m430	127	26	dried	dry	VBN
cord-104282-90t1m430	127	27	.	.	.
cord-104282-90t1m430	128	1	The	the	DT
cord-104282-90t1m430	128	2	resultant	resultant	JJ
cord-104282-90t1m430	128	3	pellet	pellet	NN
cord-104282-90t1m430	128	4	was	be	VBD
cord-104282-90t1m430	128	5	suspended	suspend	VBN
cord-104282-90t1m430	128	6	in	in	IN
cord-104282-90t1m430	128	7	100	100	CD
cord-104282-90t1m430	128	8	/	/	SYM
cord-104282-90t1m430	128	9	d	d	NN
cord-104282-90t1m430	128	10	of	of	IN
cord-104282-90t1m430	128	11	SDS	SDS	NNP
cord-104282-90t1m430	128	12	-	-	HYPH
cord-104282-90t1m430	128	13	PAGE	page	NN
cord-104282-90t1m430	128	14	sample	sample	NN
cord-104282-90t1m430	128	15	buffer	buffer	NN
cord-104282-90t1m430	128	16	.	.	.
cord-104282-90t1m430	129	1	All	all	DT
cord-104282-90t1m430	129	2	procedures	procedure	NNS
cord-104282-90t1m430	129	3	were	be	VBD
cord-104282-90t1m430	129	4	done	do	VBN
cord-104282-90t1m430	129	5	at	at	IN
cord-104282-90t1m430	129	6	room	room	NN
cord-104282-90t1m430	129	7	temperature	temperature	NN
cord-104282-90t1m430	129	8	.	.	.
cord-104282-90t1m430	130	1	Proteins	protein	NNS
cord-104282-90t1m430	130	2	were	be	VBD
cord-104282-90t1m430	130	3	separated	separate	VBN
cord-104282-90t1m430	130	4	on	on	IN
cord-104282-90t1m430	130	5	8.5	8.5	CD
cord-104282-90t1m430	130	6	%	%	NN
cord-104282-90t1m430	130	7	polyacrylamide	polyacrylamide	NN
cord-104282-90t1m430	130	8	gels	gel	NNS
cord-104282-90t1m430	130	9	(	(	-LRB-
cord-104282-90t1m430	130	10	20	20	CD
cord-104282-90t1m430	130	11	)	)	-RRB-
cord-104282-90t1m430	130	12	and	and	CC
cord-104282-90t1m430	130	13	electrophoretically	electrophoretically	RB
cord-104282-90t1m430	130	14	transferred	transfer	VBN
cord-104282-90t1m430	130	15	to	to	IN
cord-104282-90t1m430	130	16	a	a	DT
cord-104282-90t1m430	130	17	dora	dora	NNP
cord-104282-90t1m430	130	18	-	-	HYPH
cord-104282-90t1m430	130	19	pore	pore	NN
cord-104282-90t1m430	130	20	membrane	membrane	NN
cord-104282-90t1m430	130	21	according	accord	VBG
cord-104282-90t1m430	130	22	to	to	IN
cord-104282-90t1m430	130	23	the	the	DT
cord-104282-90t1m430	130	24	method	method	NN
cord-104282-90t1m430	130	25	of	of	IN
cord-104282-90t1m430	130	26	Burnette	Burnette	NNP
cord-104282-90t1m430	130	27	(	(	-LRB-
cord-104282-90t1m430	130	28	8)	8)	NNP
cord-104282-90t1m430	130	29	using	use	VBG
cord-104282-90t1m430	130	30	a	a	DT
cord-104282-90t1m430	130	31	semidry	semidry	JJ
cord-104282-90t1m430	130	32	transfer	transfer	NN
cord-104282-90t1m430	130	33	blotter	blotter	NN
cord-104282-90t1m430	130	34	for	for	IN
cord-104282-90t1m430	130	35	2.5	2.5	CD
cord-104282-90t1m430	130	36	h	h	NN
cord-104282-90t1m430	130	37	at	at	IN
cord-104282-90t1m430	130	38	36	36	CD
cord-104282-90t1m430	130	39	V.	v.	NN
cord-104282-90t1m430	130	40	After	after	IN
cord-104282-90t1m430	130	41	blocking	block	VBG
cord-104282-90t1m430	130	42	with	with	IN
cord-104282-90t1m430	130	43	3	3	CD
cord-104282-90t1m430	130	44	%	%	NN
cord-104282-90t1m430	130	45	skim	skim	NN
cord-104282-90t1m430	130	46	milk	milk	NN
cord-104282-90t1m430	130	47	in	in	IN
cord-104282-90t1m430	130	48	TBS	TBS	NNP
cord-104282-90t1m430	130	49	for	for	IN
cord-104282-90t1m430	130	50	1.5	1.5	CD
cord-104282-90t1m430	130	51	h	h	NN
cord-104282-90t1m430	130	52	,	,	,
cord-104282-90t1m430	130	53	blots	blot	NNS
cord-104282-90t1m430	130	54	were	be	VBD
cord-104282-90t1m430	130	55	incubated	incubate	VBN
cord-104282-90t1m430	130	56	with	with	IN
cord-104282-90t1m430	130	57	primary	primary	JJ
cord-104282-90t1m430	130	58	antibody	antibody	NN
cord-104282-90t1m430	130	59	in	in	IN
cord-104282-90t1m430	130	60	3	3	CD
cord-104282-90t1m430	130	61	%	%	NN
cord-104282-90t1m430	130	62	skim	skim	NN
cord-104282-90t1m430	130	63	milk/	milk/	IN
cord-104282-90t1m430	130	64	TBS	TBS	NNP
cord-104282-90t1m430	130	65	for	for	IN
cord-104282-90t1m430	130	66	1.5	1.5	CD
cord-104282-90t1m430	130	67	h	h	NN
cord-104282-90t1m430	130	68	,	,	,
cord-104282-90t1m430	130	69	and	and	CC
cord-104282-90t1m430	130	70	washed	wash	VBD
cord-104282-90t1m430	130	71	four	four	CD
cord-104282-90t1m430	130	72	times	time	NNS
cord-104282-90t1m430	130	73	(	(	-LRB-
cord-104282-90t1m430	130	74	5	5	CD
cord-104282-90t1m430	130	75	rain	rain	NN
cord-104282-90t1m430	130	76	each	each	DT
cord-104282-90t1m430	130	77	)	)	-RRB-
cord-104282-90t1m430	130	78	with	with	IN
cord-104282-90t1m430	130	79	0.05	0.05	CD
cord-104282-90t1m430	130	80	%	%	NN
cord-104282-90t1m430	130	81	Tween-20	Tween-20	NNP
cord-104282-90t1m430	130	82	in	in	IN
cord-104282-90t1m430	130	83	TBS	TBS	NNP
cord-104282-90t1m430	130	84	.	.	.
cord-104282-90t1m430	131	1	They	-PRON-	PRP
cord-104282-90t1m430	131	2	were	be	VBD
cord-104282-90t1m430	131	3	then	then	RB
cord-104282-90t1m430	131	4	incubated	incubate	VBN
cord-104282-90t1m430	131	5	with	with	IN
cord-104282-90t1m430	131	6	secondary	secondary	JJ
cord-104282-90t1m430	131	7	antibody	antibody	NN
cord-104282-90t1m430	131	8	(	(	-LRB-
cord-104282-90t1m430	131	9	pemxidaseconjugated	pemxidaseconjugated	NNP
cord-104282-90t1m430	131	10	goat	goat	NNP
cord-104282-90t1m430	131	11	anti	anti	JJ
cord-104282-90t1m430	131	12	-	-	JJ
cord-104282-90t1m430	131	13	rabbit	rabbit	JJ
cord-104282-90t1m430	131	14	IgG	igg	NN
cord-104282-90t1m430	131	15	or	or	CC
cord-104282-90t1m430	131	16	anti	anti	JJ
cord-104282-90t1m430	131	17	-	-	JJ
cord-104282-90t1m430	131	18	mouse	mouse	JJ
cord-104282-90t1m430	131	19	IgG	igg	NN
cord-104282-90t1m430	131	20	)	)	-RRB-
cord-104282-90t1m430	131	21	in	in	IN
cord-104282-90t1m430	131	22	0.05	0.05	CD
cord-104282-90t1m430	131	23	%	%	NN
cord-104282-90t1m430	131	24	Tween-20/	Tween-20/	NNP
cord-104282-90t1m430	131	25	TBS	TBS	NNP
cord-104282-90t1m430	131	26	for	for	IN
cord-104282-90t1m430	131	27	1.5	1.5	CD
cord-104282-90t1m430	131	28	h	h	NN
cord-104282-90t1m430	131	29	,	,	,
cord-104282-90t1m430	131	30	followed	follow	VBN
cord-104282-90t1m430	131	31	by	by	IN
cord-104282-90t1m430	131	32	four	four	CD
cord-104282-90t1m430	131	33	washes	wash	NNS
cord-104282-90t1m430	131	34	(	(	-LRB-
cord-104282-90t1m430	131	35	5	5	CD
cord-104282-90t1m430	131	36	rain	rain	NN
cord-104282-90t1m430	131	37	each	each	DT
cord-104282-90t1m430	131	38	)	)	-RRB-
cord-104282-90t1m430	131	39	with	with	IN
cord-104282-90t1m430	131	40	0.05	0.05	CD
cord-104282-90t1m430	131	41	%	%	NN
cord-104282-90t1m430	131	42	Tween-20	Tween-20	NNP
cord-104282-90t1m430	131	43	/	/	SYM
cord-104282-90t1m430	131	44	TBS	tbs	NN
cord-104282-90t1m430	131	45	.	.	.
cord-104282-90t1m430	132	1	Blots	blot	NNS
cord-104282-90t1m430	132	2	were	be	VBD
cord-104282-90t1m430	132	3	stained	stain	VBN
cord-104282-90t1m430	132	4	using	use	VBG
cord-104282-90t1m430	132	5	the	the	DT
cord-104282-90t1m430	132	6	enhanced	enhance	VBN
cord-104282-90t1m430	132	7	chemiluminescence	chemiluminescence	NN
cord-104282-90t1m430	132	8	Western	western	JJ
cord-104282-90t1m430	132	9	blotting	blot	VBG
cord-104282-90t1m430	132	10	detection	detection	NN
cord-104282-90t1m430	132	11	system	system	NN
cord-104282-90t1m430	132	12	(	(	-LRB-
cord-104282-90t1m430	132	13	Amersham	Amersham	NNP
cord-104282-90t1m430	132	14	Corp.	Corp.	NNP
cord-104282-90t1m430	132	15	,	,	,
cord-104282-90t1m430	132	16	Arlington	Arlington	NNP
cord-104282-90t1m430	132	17	Heights	Heights	NNP
cord-104282-90t1m430	132	18	,	,	,
cord-104282-90t1m430	132	19	IL	IL	NNP
cord-104282-90t1m430	132	20	)	)	-RRB-
cord-104282-90t1m430	132	21	.	.	.
cord-104282-90t1m430	133	1	Protein	protein	NN
cord-104282-90t1m430	133	2	bands	band	NNS
cord-104282-90t1m430	133	3	were	be	VBD
cord-104282-90t1m430	133	4	quantitated	quantitate	VBN
cord-104282-90t1m430	133	5	using	use	VBG
cord-104282-90t1m430	133	6	an	an	DT
cord-104282-90t1m430	133	7	enhanced	enhance	VBN
cord-104282-90t1m430	133	8	laser	laser	NN
cord-104282-90t1m430	133	9	densitometer	densitometer	NN
cord-104282-90t1m430	133	10	(	(	-LRB-
cord-104282-90t1m430	133	11	LKB	LKB	NNP
cord-104282-90t1m430	133	12	Instruments	Instruments	NNPS
cord-104282-90t1m430	133	13	Inc.	Inc.	NNP
cord-104282-90t1m430	133	14	,	,	,
cord-104282-90t1m430	133	15	Bromma	Bromma	NNP
cord-104282-90t1m430	133	16	,	,	,
cord-104282-90t1m430	133	17	Sweden	Sweden	NNP
cord-104282-90t1m430	133	18	)	)	-RRB-
cord-104282-90t1m430	133	19	to	to	TO
cord-104282-90t1m430	133	20	evaluate	evaluate	VB
cord-104282-90t1m430	133	21	the	the	DT
cord-104282-90t1m430	133	22	relative	relative	JJ
cord-104282-90t1m430	133	23	level	level	NN
cord-104282-90t1m430	133	24	of	of	IN
cord-104282-90t1m430	133	25	proteins	protein	NNS
cord-104282-90t1m430	133	26	in	in	IN
cord-104282-90t1m430	133	27	each	each	DT
cord-104282-90t1m430	133	28	subcellular	subcellular	JJ
cord-104282-90t1m430	133	29	fraction	fraction	NN
cord-104282-90t1m430	133	30	.	.	.
cord-104282-90t1m430	134	1	The	the	DT
cord-104282-90t1m430	134	2	densitometric	densitometric	JJ
cord-104282-90t1m430	134	3	scan	scan	JJ
cord-104282-90t1m430	134	4	value	value	NN
cord-104282-90t1m430	134	5	was	be	VBD
cord-104282-90t1m430	134	6	then	then	RB
cord-104282-90t1m430	134	7	estimated	estimate	VBN
cord-104282-90t1m430	134	8	by	by	IN
cord-104282-90t1m430	134	9	multiplying	multiply	VBG
cord-104282-90t1m430	134	10	the	the	DT
cord-104282-90t1m430	134	11	relative	relative	JJ
cord-104282-90t1m430	134	12	level	level	NN
cord-104282-90t1m430	134	13	by	by	IN
cord-104282-90t1m430	134	14	the	the	DT
cord-104282-90t1m430	134	15	protein	protein	NN
cord-104282-90t1m430	134	16	yield	yield	NN
cord-104282-90t1m430	134	17	(	(	-LRB-
cord-104282-90t1m430	134	18	milligrams	milligram	NNS
cord-104282-90t1m430	134	19	of	of	IN
cord-104282-90t1m430	134	20	protein	protein	NN
cord-104282-90t1m430	134	21	)	)	-RRB-
cord-104282-90t1m430	134	22	in	in	IN
cord-104282-90t1m430	134	23	corresponding	correspond	VBG
cord-104282-90t1m430	134	24	subcellular	subcellular	JJ
cord-104282-90t1m430	134	25	fraction	fraction	NN
cord-104282-90t1m430	134	26	.	.	.
cord-104282-90t1m430	135	1	In	in	IN
cord-104282-90t1m430	135	2	calculating	calculate	VBG
cord-104282-90t1m430	135	3	the	the	DT
cord-104282-90t1m430	135	4	percent	percent	NN
cord-104282-90t1m430	135	5	distribution	distribution	NN
cord-104282-90t1m430	135	6	of	of	IN
cord-104282-90t1m430	135	7	each	each	DT
cord-104282-90t1m430	135	8	immunodetectable	immunodetectable	JJ
cord-104282-90t1m430	135	9	protein	protein	NN
cord-104282-90t1m430	135	10	,	,	,
cord-104282-90t1m430	135	11	the	the	DT
cord-104282-90t1m430	135	12	densitometric	densitometric	JJ
cord-104282-90t1m430	135	13	scan	scan	JJ
cord-104282-90t1m430	135	14	value	value	NN
cord-104282-90t1m430	135	15	of	of	IN
cord-104282-90t1m430	135	16	the	the	DT
cord-104282-90t1m430	135	17	homogenate	homogenate	NNP
cord-104282-90t1m430	135	18	was	be	VBD
cord-104282-90t1m430	135	19	defined	define	VBN
cord-104282-90t1m430	135	20	as	as	IN
cord-104282-90t1m430	135	21	100	100	CD
cord-104282-90t1m430	135	22	%	%	NN
cord-104282-90t1m430	135	23	.	.	.
cord-104282-90t1m430	136	1	All	all	DT
cord-104282-90t1m430	136	2	procedures	procedure	NNS
cord-104282-90t1m430	136	3	,	,	,
cord-104282-90t1m430	136	4	except	except	IN
cord-104282-90t1m430	136	5	for	for	IN
cord-104282-90t1m430	136	6	the	the	DT
cord-104282-90t1m430	136	7	incubation	incubation	NN
cord-104282-90t1m430	136	8	with	with	IN
cord-104282-90t1m430	136	9	antibodies	antibody	NNS
cord-104282-90t1m430	136	10	or	or	CC
cord-104282-90t1m430	136	11	FITCconjugated	fitcconjugated	JJ
cord-104282-90t1m430	136	12	lectin	lectin	NN
cord-104282-90t1m430	136	13	at	at	IN
cord-104282-90t1m430	136	14	37	37	CD
cord-104282-90t1m430	136	15	°	°	NN
cord-104282-90t1m430	136	16	C	c	NN
cord-104282-90t1m430	136	17	,	,	,
cord-104282-90t1m430	136	18	were	be	VBD
cord-104282-90t1m430	136	19	carried	carry	VBN
cord-104282-90t1m430	136	20	out	out	RP
cord-104282-90t1m430	136	21	at	at	IN
cord-104282-90t1m430	136	22	room	room	NN
cord-104282-90t1m430	136	23	temperature	temperature	NN
cord-104282-90t1m430	136	24	.	.	.
cord-104282-90t1m430	137	1	Cells	cell	NNS
cord-104282-90t1m430	137	2	grown	grow	VBN
cord-104282-90t1m430	137	3	on	on	IN
cord-104282-90t1m430	137	4	coverslips	coverslip	NNS
cord-104282-90t1m430	137	5	were	be	VBD
cord-104282-90t1m430	137	6	washed	wash	VBN
cord-104282-90t1m430	137	7	gently	gently	RB
cord-104282-90t1m430	137	8	three	three	CD
cord-104282-90t1m430	137	9	times	time	NNS
cord-104282-90t1m430	137	10	(	(	-LRB-
cord-104282-90t1m430	137	11	5	5	CD
cord-104282-90t1m430	137	12	rain	rain	NN
cord-104282-90t1m430	137	13	each	each	DT
cord-104282-90t1m430	137	14	)	)	-RRB-
cord-104282-90t1m430	137	15	with	with	IN
cord-104282-90t1m430	137	16	PBS	PBS	NNP
cord-104282-90t1m430	137	17	,	,	,
cord-104282-90t1m430	137	18	fixed	fix	VBN
cord-104282-90t1m430	137	19	with	with	IN
cord-104282-90t1m430	137	20	4	4	CD
cord-104282-90t1m430	137	21	%	%	NN
cord-104282-90t1m430	137	22	paraformaldehyde	paraformaldehyde	NNP
cord-104282-90t1m430	137	23	in	in	IN
cord-104282-90t1m430	137	24	PBS	PBS	NNP
cord-104282-90t1m430	137	25	containing	contain	VBG
cord-104282-90t1m430	137	26	1	1	CD
cord-104282-90t1m430	137	27	mM	mm	CD
cord-104282-90t1m430	137	28	CaC12	cac12	NN
cord-104282-90t1m430	137	29	and	and	CC
cord-104282-90t1m430	137	30	0.5	0.5	CD
cord-104282-90t1m430	137	31	mM	mm	CD
cord-104282-90t1m430	138	1	MgCI2	MgCI2	NNP
cord-104282-90t1m430	138	2	[	[	-LRB-
cord-104282-90t1m430	138	3	PBS(+	PBS(+	NNP
cord-104282-90t1m430	138	4	)	)	-RRB-
cord-104282-90t1m430	138	5	]	]	-RRB-
cord-104282-90t1m430	138	6	for	for	IN
cord-104282-90t1m430	138	7	20	20	CD
cord-104282-90t1m430	138	8	rain	rain	NN
cord-104282-90t1m430	138	9	,	,	,
cord-104282-90t1m430	138	10	and	and	CC
cord-104282-90t1m430	138	11	permeabilized	permeabilize	VBN
cord-104282-90t1m430	138	12	with	with	IN
cord-104282-90t1m430	138	13	0.1	0.1	CD
cord-104282-90t1m430	138	14	%	%	NN
cord-104282-90t1m430	138	15	Triton	Triton	NNP
cord-104282-90t1m430	138	16	X-100	x-100	NN
cord-104282-90t1m430	138	17	in	in	IN
cord-104282-90t1m430	138	18	PBS(+	pbs(+	CD
cord-104282-90t1m430	138	19	)	)	-RRB-
cord-104282-90t1m430	139	1	for	for	IN
cord-104282-90t1m430	139	2	1	1	CD
cord-104282-90t1m430	139	3	h.	h.	NN
cord-104282-90t1m430	140	1	They	-PRON-	PRP
cord-104282-90t1m430	140	2	were	be	VBD
cord-104282-90t1m430	140	3	then	then	RB
cord-104282-90t1m430	140	4	rinsed	rinse	VBN
cord-104282-90t1m430	140	5	twice	twice	RB
cord-104282-90t1m430	140	6	with	with	IN
cord-104282-90t1m430	140	7	PBS(+	PBS(+	NNP
cord-104282-90t1m430	140	8	)	)	-RRB-
cord-104282-90t1m430	140	9	,	,	,
cord-104282-90t1m430	140	10	and	and	CC
cord-104282-90t1m430	140	11	incubated	incubate	VBN
cord-104282-90t1m430	140	12	with	with	IN
cord-104282-90t1m430	140	13	2	2	CD
cord-104282-90t1m430	140	14	%	%	NN
cord-104282-90t1m430	140	15	FBS	FBS	NNP
cord-104282-90t1m430	140	16	in	in	IN
cord-104282-90t1m430	140	17	PBS(+	PBS(+	NNP
cord-104282-90t1m430	140	18	)	)	-RRB-
cord-104282-90t1m430	140	19	to	to	TO
cord-104282-90t1m430	140	20	block	block	VB
cord-104282-90t1m430	140	21	nonspecific	nonspecific	JJ
cord-104282-90t1m430	140	22	binding	binding	NN
cord-104282-90t1m430	140	23	of	of	IN
cord-104282-90t1m430	140	24	primary	primary	JJ
cord-104282-90t1m430	140	25	antibodies	antibody	NNS
cord-104282-90t1m430	140	26	for	for	IN
cord-104282-90t1m430	140	27	1	1	CD
cord-104282-90t1m430	140	28	h	h	NN
cord-104282-90t1m430	140	29	followed	follow	VBN
cord-104282-90t1m430	140	30	by	by	IN
cord-104282-90t1m430	140	31	45	45	CD
cord-104282-90t1m430	140	32	rain	rain	NN
cord-104282-90t1m430	140	33	incubation	incubation	NN
cord-104282-90t1m430	140	34	with	with	IN
cord-104282-90t1m430	140	35	primary	primary	JJ
cord-104282-90t1m430	140	36	antibody	antibody	NN
cord-104282-90t1m430	140	37	in	in	IN
cord-104282-90t1m430	140	38	2	2	CD
cord-104282-90t1m430	140	39	%	%	NN
cord-104282-90t1m430	140	40	FBS	FBS	NNP
cord-104282-90t1m430	140	41	/	/	SYM
cord-104282-90t1m430	140	42	PBS(+	PBS(+	NNP
cord-104282-90t1m430	140	43	)	)	-RRB-
cord-104282-90t1m430	140	44	.	.	.
cord-104282-90t1m430	141	1	After	after	IN
cord-104282-90t1m430	141	2	washing	wash	VBG
cord-104282-90t1m430	141	3	four	four	CD
cord-104282-90t1m430	141	4	times	time	NNS
cord-104282-90t1m430	141	5	(	(	-LRB-
cord-104282-90t1m430	141	6	5	5	CD
cord-104282-90t1m430	141	7	rain	rain	NN
cord-104282-90t1m430	141	8	each	each	DT
cord-104282-90t1m430	141	9	)	)	-RRB-
cord-104282-90t1m430	141	10	with	with	IN
cord-104282-90t1m430	141	11	PBS(+	pbs(+	CD
cord-104282-90t1m430	141	12	)	)	-RRB-
cord-104282-90t1m430	141	13	,	,	,
cord-104282-90t1m430	141	14	cells	cell	NNS
cord-104282-90t1m430	141	15	were	be	VBD
cord-104282-90t1m430	141	16	incubated	incubate	VBN
cord-104282-90t1m430	141	17	with	with	IN
cord-104282-90t1m430	141	18	secondary	secondary	JJ
cord-104282-90t1m430	141	19	antibody	antibody	NN
cord-104282-90t1m430	141	20	in	in	IN
cord-104282-90t1m430	141	21	2	2	CD
cord-104282-90t1m430	141	22	%	%	NN
cord-104282-90t1m430	141	23	FBS	FBS	NNP
cord-104282-90t1m430	141	24	/	/	SYM
cord-104282-90t1m430	141	25	PBS(+	PBS(+	NNP
cord-104282-90t1m430	141	26	)	)	-RRB-
cord-104282-90t1m430	141	27	for	for	IN
cord-104282-90t1m430	141	28	45	45	CD
cord-104282-90t1m430	141	29	min	min	NN
cord-104282-90t1m430	141	30	.	.	.
cord-104282-90t1m430	142	1	For	for	IN
cord-104282-90t1m430	142	2	localization	localization	NN
cord-104282-90t1m430	142	3	of	of	IN
cord-104282-90t1m430	142	4	the	the	DT
cord-104282-90t1m430	142	5	Golgi	Golgi	NNP
cord-104282-90t1m430	142	6	complex	complex	NN
cord-104282-90t1m430	142	7	,	,	,
cord-104282-90t1m430	142	8	cells	cell	NNS
cord-104282-90t1m430	142	9	were	be	VBD
cord-104282-90t1m430	142	10	incubated	incubate	VBN
cord-104282-90t1m430	142	11	with	with	IN
cord-104282-90t1m430	142	12	FITCconjugated	fitcconjugated	JJ
cord-104282-90t1m430	142	13	wheat	wheat	NN
cord-104282-90t1m430	142	14	germ	germ	NNP
cord-104282-90t1m430	142	15	agglutinin	agglutinin	NNS
cord-104282-90t1m430	142	16	in	in	IN
cord-104282-90t1m430	142	17	2	2	CD
cord-104282-90t1m430	142	18	%	%	NN
cord-104282-90t1m430	142	19	FBS	FBS	NNP
cord-104282-90t1m430	142	20	/	/	SYM
cord-104282-90t1m430	142	21	PBS(+	PBS(+	NNP
cord-104282-90t1m430	142	22	)	)	-RRB-
cord-104282-90t1m430	142	23	for	for	IN
cord-104282-90t1m430	142	24	45	45	CD
cord-104282-90t1m430	142	25	rain	rain	NN
cord-104282-90t1m430	142	26	.	.	.
cord-104282-90t1m430	143	1	After	after	IN
cord-104282-90t1m430	143	2	washing	wash	VBG
cord-104282-90t1m430	143	3	with	with	IN
cord-104282-90t1m430	143	4	PBS(+	PBS(+	NNP
cord-104282-90t1m430	143	5	)	)	-RRB-
cord-104282-90t1m430	143	6	,	,	,
cord-104282-90t1m430	143	7	they	-PRON-	PRP
cord-104282-90t1m430	143	8	were	be	VBD
cord-104282-90t1m430	143	9	then	then	RB
cord-104282-90t1m430	143	10	mounted	mount	VBN
cord-104282-90t1m430	143	11	on	on	IN
cord-104282-90t1m430	143	12	glass	glass	NN
cord-104282-90t1m430	143	13	slides	slide	NNS
cord-104282-90t1m430	143	14	with	with	IN
cord-104282-90t1m430	143	15	90	90	CD
cord-104282-90t1m430	143	16	%	%	NN
cord-104282-90t1m430	143	17	glycerol	glycerol	NN
cord-104282-90t1m430	143	18	in	in	IN
cord-104282-90t1m430	143	19	PBS	PBS	NNP
cord-104282-90t1m430	143	20	containing	contain	VBG
cord-104282-90t1m430	143	21	1	1	CD
cord-104282-90t1m430	143	22	m~/ml	m~/ml	JJ
cord-104282-90t1m430	143	23	paraphenylenediamine	paraphenylenediamine	NN
cord-104282-90t1m430	143	24	,	,	,
cord-104282-90t1m430	143	25	examined	examine	VBN
cord-104282-90t1m430	143	26	,	,	,
cord-104282-90t1m430	143	27	and	and	CC
cord-104282-90t1m430	143	28	photographed	photograph	VBN
cord-104282-90t1m430	143	29	on	on	IN
cord-104282-90t1m430	143	30	a	a	DT
cord-104282-90t1m430	143	31	microscope	microscope	NN
cord-104282-90t1m430	143	32	(	(	-LRB-
cord-104282-90t1m430	143	33	BH-2	BH-2	NNP
cord-104282-90t1m430	143	34	;	;	:
cord-104282-90t1m430	143	35	Olympus	Olympus	NNP
cord-104282-90t1m430	143	36	Corp.	Corp.	NNP
cord-104282-90t1m430	143	37	,	,	,
cord-104282-90t1m430	143	38	Tokyo	Tokyo	NNP
cord-104282-90t1m430	143	39	,	,	,
cord-104282-90t1m430	143	40	Japan	Japan	NNP
cord-104282-90t1m430	143	41	)	)	-RRB-
cord-104282-90t1m430	143	42	with	with	IN
cord-104282-90t1m430	143	43	Ektachrome	Ektachrome	NNP
cord-104282-90t1m430	143	44	400	400	CD
cord-104282-90t1m430	143	45	film	film	NN
cord-104282-90t1m430	143	46	(	(	-LRB-
cord-104282-90t1m430	143	47	Kodak	Kodak	NNP
cord-104282-90t1m430	143	48	,	,	,
cord-104282-90t1m430	143	49	Rochester	Rochester	NNP
cord-104282-90t1m430	143	50	,	,	,
cord-104282-90t1m430	143	51	NY	NY	NNP
cord-104282-90t1m430	143	52	)	)	-RRB-
cord-104282-90t1m430	143	53	.	.	.
cord-104282-90t1m430	144	1	Frozen	frozen	JJ
cord-104282-90t1m430	144	2	ultramicrotomy	ultramicrotomy	NN
cord-104282-90t1m430	144	3	was	be	VBD
cord-104282-90t1m430	144	4	performed	perform	VBN
cord-104282-90t1m430	144	5	as	as	IN
cord-104282-90t1m430	144	6	described	describe	VBN
cord-104282-90t1m430	144	7	by	by	IN
cord-104282-90t1m430	144	8	Tokuyasu	Tokuyasu	NNP
cord-104282-90t1m430	144	9	(	(	-LRB-
cord-104282-90t1m430	144	10	46	46	CD
cord-104282-90t1m430	144	11	)	)	-RRB-
cord-104282-90t1m430	144	12	.	.	.
cord-104282-90t1m430	145	1	Transfected	transfected	JJ
cord-104282-90t1m430	145	2	cells	cell	NNS
cord-104282-90t1m430	145	3	were	be	VBD
cord-104282-90t1m430	145	4	harvested	harvest	VBN
cord-104282-90t1m430	145	5	by	by	IN
cord-104282-90t1m430	145	6	centrifugation	centrifugation	NN
cord-104282-90t1m430	145	7	at	at	IN
cord-104282-90t1m430	145	8	1,000	1,000	CD
cord-104282-90t1m430	145	9	g	g	NN
cord-104282-90t1m430	145	10	for	for	IN
cord-104282-90t1m430	145	11	3	3	CD
cord-104282-90t1m430	145	12	rain	rain	NN
cord-104282-90t1m430	145	13	,	,	,
cord-104282-90t1m430	145	14	and	and	CC
cord-104282-90t1m430	145	15	the	the	DT
cord-104282-90t1m430	145	16	pellet	pellet	NN
cord-104282-90t1m430	145	17	was	be	VBD
cord-104282-90t1m430	145	18	fixed	fix	VBN
cord-104282-90t1m430	145	19	with	with	IN
cord-104282-90t1m430	145	20	4	4	CD
cord-104282-90t1m430	145	21	%	%	NN
cord-104282-90t1m430	145	22	paraformaldehyde	paraformaldehyde	NN
cord-104282-90t1m430	145	23	and	and	CC
cord-104282-90t1m430	145	24	0.1	0.1	CD
cord-104282-90t1m430	145	25	%	%	NN
cord-104282-90t1m430	145	26	glutaraldehyde	glutaraldehyde	VBN
cord-104282-90t1m430	145	27	dis-	dis-	IN
cord-104282-90t1m430	146	1	Cell	cell	NN
cord-104282-90t1m430	146	2	fractionation	fractionation	NN
cord-104282-90t1m430	146	3	was	be	VBD
cord-104282-90t1m430	146	4	performed	perform	VBN
cord-104282-90t1m430	146	5	as	as	IN
cord-104282-90t1m430	146	6	described	describe	VBN
cord-104282-90t1m430	146	7	in	in	IN
cord-104282-90t1m430	146	8	Materials	material	NNS
cord-104282-90t1m430	146	9	and	and	CC
cord-104282-90t1m430	146	10	Methods	method	NNS
cord-104282-90t1m430	146	11	.	.	.
cord-104282-90t1m430	147	1	In	in	IN
cord-104282-90t1m430	147	2	calculating	calculate	VBG
cord-104282-90t1m430	147	3	the	the	DT
cord-104282-90t1m430	147	4	percent	percent	NN
cord-104282-90t1m430	147	5	distribution	distribution	NN
cord-104282-90t1m430	147	6	,	,	,
cord-104282-90t1m430	147	7	each	each	DT
cord-104282-90t1m430	147	8	value	value	NN
cord-104282-90t1m430	147	9	of	of	IN
cord-104282-90t1m430	147	10	the	the	DT
cord-104282-90t1m430	147	11	homogenate	homogenate	NNP
cord-104282-90t1m430	147	12	was	be	VBD
cord-104282-90t1m430	147	13	defined	define	VBN
cord-104282-90t1m430	147	14	as	as	IN
cord-104282-90t1m430	147	15	100	100	CD
cord-104282-90t1m430	147	16	%	%	NN
cord-104282-90t1m430	147	17	.	.	.
cord-104282-90t1m430	148	1	The	the	DT
cord-104282-90t1m430	148	2	distribution	distribution	NN
cord-104282-90t1m430	148	3	of	of	IN
cord-104282-90t1m430	148	4	FP2	fp2	NN
cord-104282-90t1m430	148	5	and	and	CC
cord-104282-90t1m430	148	6	succinate	succinate	NN
cord-104282-90t1m430	148	7	-	-	HYPH
cord-104282-90t1m430	148	8	cytochrome	cytochrome	JJ
cord-104282-90t1m430	148	9	c	c	NN
cord-104282-90t1m430	148	10	reductase(SCR	reductase(SCR	NNP
cord-104282-90t1m430	148	11	)	)	-RRB-
cord-104282-90t1m430	148	12	is	be	VBZ
cord-104282-90t1m430	148	13	determined	determine	VBN
cord-104282-90t1m430	148	14	from	from	IN
cord-104282-90t1m430	148	15	the	the	DT
cord-104282-90t1m430	148	16	specific	specific	JJ
cord-104282-90t1m430	148	17	activities	activity	NNS
cord-104282-90t1m430	148	18	measured	measure	VBN
cord-104282-90t1m430	148	19	.	.	.
cord-104282-90t1m430	149	1	The	the	DT
cord-104282-90t1m430	149	2	specific	specific	JJ
cord-104282-90t1m430	149	3	activities	activity	NNS
cord-104282-90t1m430	149	4	of	of	IN
cord-104282-90t1m430	149	5	the	the	DT
cord-104282-90t1m430	149	6	homogenate	homogenate	NNP
cord-104282-90t1m430	149	7	fractions	fraction	NNS
cord-104282-90t1m430	149	8	are	be	VBP
cord-104282-90t1m430	150	1	52.3	52.3	CD
cord-104282-90t1m430	150	2	+	+	SYM
cord-104282-90t1m430	150	3	11.0	11.0	CD
cord-104282-90t1m430	150	4	nmol	nmol	NNP
cord-104282-90t1m430	150	5	cytechrome	cytechrome	NNP
cord-104282-90t1m430	150	6	c	c	NNP
cord-104282-90t1m430	150	7	reduced	reduced	NNP
cord-104282-90t1m430	150	8	/	/	SYM
cord-104282-90t1m430	150	9	rain	rain	NN
cord-104282-90t1m430	150	10	per	per	IN
cord-104282-90t1m430	150	11	mg	mg	NN
cord-104282-90t1m430	150	12	protein	protein	NN
cord-104282-90t1m430	150	13	(	(	-LRB-
cord-104282-90t1m430	150	14	the	the	DT
cord-104282-90t1m430	150	15	mean	mean	NN
cord-104282-90t1m430	150	16	+	+	CC
cord-104282-90t1m430	150	17	SD	sd	NN
cord-104282-90t1m430	150	18	n	n	NN
cord-104282-90t1m430	150	19	=	=	SYM
cord-104282-90t1m430	150	20	4	4	CD
cord-104282-90t1m430	150	21	)	)	-RRB-
cord-104282-90t1m430	150	22	and	and	CC
cord-104282-90t1m430	150	23	26.9	26.9	CD
cord-104282-90t1m430	150	24	+	+	SYM
cord-104282-90t1m430	150	25	2.8	2.8	CD
cord-104282-90t1m430	150	26	nmol	nmol	JJ
cord-104282-90t1m430	150	27	cytoehrome	cytoehrome	NNP
cord-104282-90t1m430	150	28	c	c	NNP
cord-104282-90t1m430	150	29	reduced	reduced	NNP
cord-104282-90t1m430	150	30	/	/	SYM
cord-104282-90t1m430	150	31	min	min	NN
cord-104282-90t1m430	150	32	per	per	IN
cord-104282-90t1m430	150	33	mg	mg	NN
cord-104282-90t1m430	150	34	protein	protein	NN
cord-104282-90t1m430	150	35	for	for	IN
cord-104282-90t1m430	150	36	FP2	fp2	NN
cord-104282-90t1m430	150	37	and	and	CC
cord-104282-90t1m430	150	38	succinate	succinate	NN
cord-104282-90t1m430	150	39	-	-	HYPH
cord-104282-90t1m430	150	40	cytochrome	cytochrome	JJ
cord-104282-90t1m430	150	41	c	c	NN
cord-104282-90t1m430	150	42	reductase	reductase	NN
cord-104282-90t1m430	150	43	,	,	,
cord-104282-90t1m430	150	44	respectively	respectively	RB
cord-104282-90t1m430	150	45	.	.	.
cord-104282-90t1m430	151	1	In	in	IN
cord-104282-90t1m430	151	2	the	the	DT
cord-104282-90t1m430	151	3	previous	previous	JJ
cord-104282-90t1m430	151	4	study	study	NN
cord-104282-90t1m430	151	5	(	(	-LRB-
cord-104282-90t1m430	151	6	24	24	CD
cord-104282-90t1m430	151	7	)	)	-RRB-
cord-104282-90t1m430	151	8	,	,	,
cord-104282-90t1m430	151	9	we	-PRON-	PRP
cord-104282-90t1m430	151	10	cloned	clone	VBD
cord-104282-90t1m430	151	11	and	and	CC
cord-104282-90t1m430	151	12	sequenced	sequence	VBD
cord-104282-90t1m430	151	13	the	the	DT
cord-104282-90t1m430	151	14	fulllength	fulllength	NN
cord-104282-90t1m430	151	15	cDNA	cdna	NN
cord-104282-90t1m430	151	16	for	for	IN
cord-104282-90t1m430	151	17	rat	rat	NN
cord-104282-90t1m430	151	18	msALDH	msALDH	NNP
cord-104282-90t1m430	151	19	.	.	.
cord-104282-90t1m430	152	1	The	the	DT
cord-104282-90t1m430	152	2	nucleotide	nucleotide	JJ
cord-104282-90t1m430	152	3	sequence	sequence	NN
cord-104282-90t1m430	152	4	predicts	predict	VBZ
cord-104282-90t1m430	152	5	a	a	DT
cord-104282-90t1m430	152	6	polypeptide	polypeptide	NN
cord-104282-90t1m430	152	7	of	of	IN
cord-104282-90t1m430	152	8	484	484	CD
cord-104282-90t1m430	152	9	amino	amino	JJ
cord-104282-90t1m430	152	10	acids	acid	NNS
cord-104282-90t1m430	152	11	,	,	,
cord-104282-90t1m430	152	12	and	and	CC
cord-104282-90t1m430	152	13	the	the	DT
cord-104282-90t1m430	152	14	most	most	RBS
cord-104282-90t1m430	152	15	characteristic	characteristic	JJ
cord-104282-90t1m430	152	16	feature	feature	NN
cord-104282-90t1m430	152	17	of	of	IN
cord-104282-90t1m430	152	18	this	this	DT
cord-104282-90t1m430	152	19	membrane	membrane	NN
cord-104282-90t1m430	152	20	-	-	HYPH
cord-104282-90t1m430	152	21	bound	bind	VBN
cord-104282-90t1m430	152	22	ALDH	ALDH	NNP
cord-104282-90t1m430	152	23	is	be	VBZ
cord-104282-90t1m430	152	24	carboxyl	carboxyl	NN
cord-104282-90t1m430	152	25	-	-	HYPH
cord-104282-90t1m430	152	26	terminal	terminal	NN
cord-104282-90t1m430	152	27	35	35	CD
cord-104282-90t1m430	152	28	amino	amino	NN
cord-104282-90t1m430	152	29	acids	acid	NNS
cord-104282-90t1m430	152	30	,	,	,
cord-104282-90t1m430	152	31	consisting	consist	VBG
cord-104282-90t1m430	152	32	of	of	IN
cord-104282-90t1m430	152	33	a	a	DT
cord-104282-90t1m430	152	34	stem	stem	NN
cord-104282-90t1m430	152	35	region	region	NN
cord-104282-90t1m430	152	36	(	(	-LRB-
cord-104282-90t1m430	152	37	amino	amino	NN
cord-104282-90t1m430	152	38	acids	acid	NNS
cord-104282-90t1m430	152	39	450	450	CD
cord-104282-90t1m430	152	40	-	-	SYM
cord-104282-90t1m430	152	41	463	463	CD
cord-104282-90t1m430	152	42	)	)	-RRB-
cord-104282-90t1m430	152	43	and	and	CC
cord-104282-90t1m430	152	44	a	a	DT
cord-104282-90t1m430	152	45	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	152	46	domain	domain	NN
cord-104282-90t1m430	152	47	(	(	-LRB-
cord-104282-90t1m430	152	48	amino	amino	NN
cord-104282-90t1m430	152	49	acids	acid	NNS
cord-104282-90t1m430	152	50	464	464	CD
cord-104282-90t1m430	152	51	-	-	SYM
cord-104282-90t1m430	152	52	480	480	CD
cord-104282-90t1m430	152	53	)	)	-RRB-
cord-104282-90t1m430	152	54	followed	follow	VBN
cord-104282-90t1m430	152	55	by	by	IN
cord-104282-90t1m430	152	56	a	a	DT
cord-104282-90t1m430	152	57	short	short	JJ
cord-104282-90t1m430	152	58	hydrophilic	hydrophilic	JJ
cord-104282-90t1m430	152	59	tail	tail	NN
cord-104282-90t1m430	152	60	region	region	NN
cord-104282-90t1m430	152	61	(	(	-LRB-
cord-104282-90t1m430	152	62	amino	amino	JJ
cord-104282-90t1m430	152	63	acids	acid	NNS
cord-104282-90t1m430	152	64	481	481	CD
cord-104282-90t1m430	152	65	-	-	SYM
cord-104282-90t1m430	152	66	484	484	CD
cord-104282-90t1m430	152	67	)	)	-RRB-
cord-104282-90t1m430	152	68	as	as	IN
cord-104282-90t1m430	152	69	shown	show	VBN
cord-104282-90t1m430	152	70	in	in	IN
cord-104282-90t1m430	152	71	Fig	Fig	NNP
cord-104282-90t1m430	152	72	.	.	.
cord-104282-90t1m430	153	1	1	1	LS
cord-104282-90t1m430	153	2	.	.	.
cord-104282-90t1m430	154	1	Since	since	IN
cord-104282-90t1m430	154	2	rat	rat	NN
cord-104282-90t1m430	154	3	cytosolic	cytosolic	JJ
cord-104282-90t1m430	154	4	tumor	tumor	NN
cord-104282-90t1m430	154	5	-	-	HYPH
cord-104282-90t1m430	154	6	associated	associate	VBN
cord-104282-90t1m430	154	7	ALDH	ALDH	NNP
cord-104282-90t1m430	154	8	,	,	,
cord-104282-90t1m430	154	9	which	which	WDT
cord-104282-90t1m430	154	10	is	be	VBZ
cord-104282-90t1m430	154	11	65.5	65.5	CD
cord-104282-90t1m430	154	12	%	%	NN
cord-104282-90t1m430	154	13	identical	identical	JJ
cord-104282-90t1m430	154	14	to	to	IN
cord-104282-90t1m430	154	15	msALDH	msALDH	NNP
cord-104282-90t1m430	154	16	(	(	-LRB-
cord-104282-90t1m430	154	17	24	24	CD
cord-104282-90t1m430	154	18	)	)	-RRB-
cord-104282-90t1m430	154	19	,	,	,
cord-104282-90t1m430	154	20	lacks	lack	VBZ
cord-104282-90t1m430	154	21	the	the	DT
cord-104282-90t1m430	154	22	carboxyl	carboxyl	NN
cord-104282-90t1m430	154	23	-	-	HYPH
cord-104282-90t1m430	154	24	terminal	terminal	NN
cord-104282-90t1m430	154	25	portion	portion	NN
cord-104282-90t1m430	154	26	,	,	,
cord-104282-90t1m430	154	27	we	-PRON-	PRP
cord-104282-90t1m430	154	28	asked	ask	VBD
cord-104282-90t1m430	154	29	whether	whether	IN
cord-104282-90t1m430	154	30	this	this	DT
cord-104282-90t1m430	154	31	portion	portion	NN
cord-104282-90t1m430	154	32	played	play	VBD
cord-104282-90t1m430	154	33	an	an	DT
cord-104282-90t1m430	154	34	important	important	JJ
cord-104282-90t1m430	154	35	role	role	NN
cord-104282-90t1m430	154	36	in	in	IN
cord-104282-90t1m430	154	37	the	the	DT
cord-104282-90t1m430	154	38	intracellular	intracellular	JJ
cord-104282-90t1m430	154	39	localization	localization	NN
cord-104282-90t1m430	154	40	of	of	IN
cord-104282-90t1m430	154	41	msALDH	msALDH	NNP
cord-104282-90t1m430	154	42	.	.	.
cord-104282-90t1m430	155	1	For	for	IN
cord-104282-90t1m430	155	2	this	this	DT
cord-104282-90t1m430	155	3	purpose	purpose	NN
cord-104282-90t1m430	155	4	,	,	,
cord-104282-90t1m430	155	5	we	-PRON-	PRP
cord-104282-90t1m430	155	6	constructed	construct	VBD
cord-104282-90t1m430	155	7	three	three	CD
cord-104282-90t1m430	155	8	mutant	mutant	JJ
cord-104282-90t1m430	155	9	proteins	protein	NNS
cord-104282-90t1m430	155	10	truncated	truncate	VBN
cord-104282-90t1m430	155	11	at	at	IN
cord-104282-90t1m430	155	12	either	either	DT
cord-104282-90t1m430	155	13	amino	amino	NN
cord-104282-90t1m430	155	14	acid	acid	NN
cord-104282-90t1m430	155	15	450	450	CD
cord-104282-90t1m430	155	16	(	(	-LRB-
cord-104282-90t1m430	155	17	msALDHA450	msALDHA450	NNP
cord-104282-90t1m430	155	18	-	-	HYPH
cord-104282-90t1m430	155	19	484	484	CD
cord-104282-90t1m430	155	20	,	,	,
cord-104282-90t1m430	155	21	which	which	WDT
cord-104282-90t1m430	155	22	corresponds	correspond	VBZ
cord-104282-90t1m430	155	23	to	to	IN
cord-104282-90t1m430	155	24	the	the	DT
cord-104282-90t1m430	155	25	tumor	tumor	NN
cord-104282-90t1m430	155	26	-	-	HYPH
cord-104282-90t1m430	155	27	associated	associate	VBN
cord-104282-90t1m430	155	28	ALDH	ALDH	NNP
cord-104282-90t1m430	155	29	in	in	IN
cord-104282-90t1m430	155	30	size	size	NN
cord-104282-90t1m430	155	31	)	)	-RRB-
cord-104282-90t1m430	155	32	,	,	,
cord-104282-90t1m430	155	33	471	471	CD
cord-104282-90t1m430	155	34	(	(	-LRB-
cord-104282-90t1m430	155	35	msALDHA471	msaldha471	CD
cord-104282-90t1m430	155	36	-	-	SYM
cord-104282-90t1m430	155	37	484	484	CD
cord-104282-90t1m430	155	38	,	,	,
cord-104282-90t1m430	155	39	deletion	deletion	NN
cord-104282-90t1m430	155	40	of	of	IN
cord-104282-90t1m430	155	41	more	more	JJR
cord-104282-90t1m430	155	42	than	than	IN
cord-104282-90t1m430	155	43	half	half	NN
cord-104282-90t1m430	155	44	of	of	IN
cord-104282-90t1m430	155	45	the	the	DT
cord-104282-90t1m430	155	46	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	155	47	domain	domain	NN
cord-104282-90t1m430	155	48	together	together	RB
cord-104282-90t1m430	155	49	with	with	IN
cord-104282-90t1m430	155	50	the	the	DT
cord-104282-90t1m430	155	51	tail	tail	NN
cord-104282-90t1m430	155	52	region	region	NN
cord-104282-90t1m430	155	53	)	)	-RRB-
cord-104282-90t1m430	155	54	,	,	,
cord-104282-90t1m430	155	55	or	or	CC
cord-104282-90t1m430	155	56	481	481	CD
cord-104282-90t1m430	155	57	(	(	-LRB-
cord-104282-90t1m430	155	58	msALDHA481	msALDHA481	NNP
cord-104282-90t1m430	155	59	-	-	SYM
cord-104282-90t1m430	155	60	484	484	CD
cord-104282-90t1m430	155	61	,	,	,
cord-104282-90t1m430	155	62	deletion	deletion	NN
cord-104282-90t1m430	155	63	of	of	IN
cord-104282-90t1m430	155	64	solely	solely	RB
cord-104282-90t1m430	155	65	the	the	DT
cord-104282-90t1m430	155	66	tail	tail	NN
cord-104282-90t1m430	155	67	region	region	NN
cord-104282-90t1m430	155	68	)	)	-RRB-
cord-104282-90t1m430	155	69	by	by	IN
cord-104282-90t1m430	155	70	oligonucleotide	oligonucleotide	NN
cord-104282-90t1m430	155	71	-	-	HYPH
cord-104282-90t1m430	155	72	directed	direct	VBN
cord-104282-90t1m430	155	73	mutagenesis	mutagenesis	NN
cord-104282-90t1m430	155	74	(	(	-LRB-
cord-104282-90t1m430	155	75	Fig	Fig	NNP
cord-104282-90t1m430	155	76	.	.	.
cord-104282-90t1m430	155	77	1	1	CD
cord-104282-90t1m430	155	78	)	)	-RRB-
cord-104282-90t1m430	155	79	.	.	.
cord-104282-90t1m430	156	1	These	these	DT
cord-104282-90t1m430	156	2	mutant	mutant	JJ
cord-104282-90t1m430	156	3	proteins	protein	NNS
cord-104282-90t1m430	156	4	and	and	CC
cord-104282-90t1m430	156	5	wild	wild	JJ
cord-104282-90t1m430	156	6	-	-	HYPH
cord-104282-90t1m430	156	7	type	type	NN
cord-104282-90t1m430	156	8	msALDH	msALDH	NNS
cord-104282-90t1m430	156	9	were	be	VBD
cord-104282-90t1m430	156	10	expressed	express	VBN
cord-104282-90t1m430	156	11	transiently	transiently	RB
cord-104282-90t1m430	156	12	in	in	IN
cord-104282-90t1m430	156	13	COS-1	COS-1	NNP
cord-104282-90t1m430	156	14	cells	cell	NNS
cord-104282-90t1m430	156	15	under	under	IN
cord-104282-90t1m430	156	16	the	the	DT
cord-104282-90t1m430	156	17	control	control	NN
cord-104282-90t1m430	156	18	of	of	IN
cord-104282-90t1m430	156	19	SV-40	SV-40	NNP
cord-104282-90t1m430	156	20	promoter	promoter	NN
cord-104282-90t1m430	156	21	in	in	IN
cord-104282-90t1m430	156	22	the	the	DT
cord-104282-90t1m430	156	23	pCD	pCD	NNP
cord-104282-90t1m430	156	24	expression	expression	NN
cord-104282-90t1m430	156	25	vector	vector	NN
cord-104282-90t1m430	156	26	.	.	.
cord-104282-90t1m430	157	1	This	this	DT
cord-104282-90t1m430	157	2	transient	transient	JJ
cord-104282-90t1m430	157	3	expression	expression	NN
cord-104282-90t1m430	157	4	system	system	NN
cord-104282-90t1m430	157	5	in	in	IN
cord-104282-90t1m430	157	6	COS-1	COS-1	NNP
cord-104282-90t1m430	157	7	cells	cell	NNS
cord-104282-90t1m430	157	8	was	be	VBD
cord-104282-90t1m430	157	9	chosen	choose	VBN
cord-104282-90t1m430	157	10	as	as	IN
cord-104282-90t1m430	157	11	the	the	DT
cord-104282-90t1m430	157	12	most	most	RBS
cord-104282-90t1m430	157	13	rapid	rapid	JJ
cord-104282-90t1m430	157	14	method	method	NN
cord-104282-90t1m430	157	15	for	for	IN
cord-104282-90t1m430	157	16	evaluating	evaluate	VBG
cord-104282-90t1m430	157	17	the	the	DT
cord-104282-90t1m430	157	18	intracellular	intracellular	JJ
cord-104282-90t1m430	157	19	localization	localization	NN
cord-104282-90t1m430	157	20	of	of	IN
cord-104282-90t1m430	157	21	expressed	express	VBN
cord-104282-90t1m430	157	22	proteins	protein	NNS
cord-104282-90t1m430	157	23	.	.	.
cord-104282-90t1m430	158	1	Cells	cell	NNS
cord-104282-90t1m430	158	2	were	be	VBD
cord-104282-90t1m430	158	3	allowed	allow	VBN
cord-104282-90t1m430	158	4	to	to	TO
cord-104282-90t1m430	158	5	express	express	VB
cord-104282-90t1m430	158	6	these	these	DT
cord-104282-90t1m430	158	7	proteins	protein	NNS
cord-104282-90t1m430	158	8	for	for	IN
cord-104282-90t1m430	158	9	44	44	CD
cord-104282-90t1m430	158	10	h	h	NN
cord-104282-90t1m430	158	11	,	,	,
cord-104282-90t1m430	158	12	harvested	harvest	VBN
cord-104282-90t1m430	158	13	,	,	,
cord-104282-90t1m430	158	14	and	and	CC
cord-104282-90t1m430	158	15	the	the	DT
cord-104282-90t1m430	158	16	expressed	express	VBN
cord-104282-90t1m430	158	17	proteins	protein	NNS
cord-104282-90t1m430	158	18	were	be	VBD
cord-104282-90t1m430	158	19	analyzed	analyze	VBN
cord-104282-90t1m430	158	20	by	by	IN
cord-104282-90t1m430	158	21	immunoblotting	immunoblotte	VBG
cord-104282-90t1m430	158	22	using	use	VBG
cord-104282-90t1m430	158	23	anti	anti	JJ
cord-104282-90t1m430	158	24	-	-	JJ
cord-104282-90t1m430	158	25	msALDH	msaldh	JJ
cord-104282-90t1m430	158	26	antibody	antibody	NN
cord-104282-90t1m430	158	27	.	.	.
cord-104282-90t1m430	159	1	As	as	IN
cord-104282-90t1m430	159	2	shown	show	VBN
cord-104282-90t1m430	159	3	in	in	IN
cord-104282-90t1m430	159	4	Fig	Fig	NNP
cord-104282-90t1m430	159	5	.	.	.
cord-104282-90t1m430	160	1	2	2	LS
cord-104282-90t1m430	161	1	A	a	LS
cord-104282-90t1m430	161	2	,	,	,
cord-104282-90t1m430	161	3	msALDH	msaldh	NN
cord-104282-90t1m430	161	4	and	and	CC
cord-104282-90t1m430	161	5	three	three	CD
cord-104282-90t1m430	161	6	truncated	truncated	JJ
cord-104282-90t1m430	161	7	proteins	protein	NNS
cord-104282-90t1m430	161	8	were	be	VBD
cord-104282-90t1m430	161	9	expressed	express	VBN
cord-104282-90t1m430	161	10	efficiently	efficiently	RB
cord-104282-90t1m430	161	11	in	in	IN
cord-104282-90t1m430	161	12	the	the	DT
cord-104282-90t1m430	161	13	total	total	JJ
cord-104282-90t1m430	161	14	homogenates	homogenate	NNS
cord-104282-90t1m430	161	15	.	.	.
cord-104282-90t1m430	162	1	As	as	IN
cord-104282-90t1m430	162	2	expected	expect	VBN
cord-104282-90t1m430	162	3	,	,	,
cord-104282-90t1m430	162	4	molecular	molecular	JJ
cord-104282-90t1m430	162	5	masses	masse	NNS
cord-104282-90t1m430	162	6	of	of	IN
cord-104282-90t1m430	162	7	the	the	DT
cord-104282-90t1m430	162	8	truncated	truncated	JJ
cord-104282-90t1m430	162	9	proteins	protein	NNS
cord-104282-90t1m430	162	10	were	be	VBD
cord-104282-90t1m430	162	11	smaller	small	JJR
cord-104282-90t1m430	162	12	than	than	IN
cord-104282-90t1m430	162	13	that	that	DT
cord-104282-90t1m430	162	14	of	of	IN
cord-104282-90t1m430	162	15	msALDH	msALDH	NNP
cord-104282-90t1m430	162	16	(	(	-LRB-
cord-104282-90t1m430	162	17	54	54	CD
cord-104282-90t1m430	162	18	kD	kd	ADD
cord-104282-90t1m430	162	19	)	)	-RRB-
cord-104282-90t1m430	162	20	.	.	.
cord-104282-90t1m430	163	1	In	in	IN
cord-104282-90t1m430	163	2	addition	addition	NN
cord-104282-90t1m430	163	3	,	,	,
cord-104282-90t1m430	163	4	no	no	DT
cord-104282-90t1m430	163	5	crossreactive	crossreactive	JJ
cord-104282-90t1m430	163	6	protein	protein	NN
cord-104282-90t1m430	163	7	was	be	VBD
cord-104282-90t1m430	163	8	detected	detect	VBN
cord-104282-90t1m430	163	9	in	in	IN
cord-104282-90t1m430	163	10	untransfected	untransfected	JJ
cord-104282-90t1m430	163	11	COS-1	COS-1	NNP
cord-104282-90t1m430	163	12	cells	cell	NNS
cord-104282-90t1m430	163	13	(	(	-LRB-
cord-104282-90t1m430	163	14	data	datum	NNS
cord-104282-90t1m430	163	15	not	not	RB
cord-104282-90t1m430	163	16	shown	show	VBN
cord-104282-90t1m430	163	17	)	)	-RRB-
cord-104282-90t1m430	163	18	,	,	,
cord-104282-90t1m430	163	19	indicating	indicate	VBG
cord-104282-90t1m430	163	20	the	the	DT
cord-104282-90t1m430	163	21	absence	absence	NN
cord-104282-90t1m430	163	22	of	of	IN
cord-104282-90t1m430	163	23	endogenous	endogenous	JJ
cord-104282-90t1m430	163	24	msALDH	msALDH	NNS
cord-104282-90t1m430	163	25	.	.	.
cord-104282-90t1m430	164	1	These	these	DT
cord-104282-90t1m430	164	2	results	result	NNS
cord-104282-90t1m430	164	3	allowed	allow	VBD
cord-104282-90t1m430	164	4	us	-PRON-	PRP
cord-104282-90t1m430	164	5	to	to	TO
cord-104282-90t1m430	164	6	investigate	investigate	VB
cord-104282-90t1m430	164	7	the	the	DT
cord-104282-90t1m430	164	8	intracellular	intracellular	JJ
cord-104282-90t1m430	164	9	localization	localization	NN
cord-104282-90t1m430	164	10	of	of	IN
cord-104282-90t1m430	164	11	msALDH	msaldh	NN
cord-104282-90t1m430	164	12	by	by	IN
cord-104282-90t1m430	164	13	transfection	transfection	NN
cord-104282-90t1m430	164	14	experiments	experiment	NNS
cord-104282-90t1m430	164	15	.	.	.
cord-104282-90t1m430	165	1	We	-PRON-	PRP
cord-104282-90t1m430	165	2	analyzed	analyze	VBD
cord-104282-90t1m430	165	3	the	the	DT
cord-104282-90t1m430	165	4	intraceUular	intraceuular	JJ
cord-104282-90t1m430	165	5	distribution	distribution	NN
cord-104282-90t1m430	165	6	of	of	IN
cord-104282-90t1m430	165	7	msALDH	msALDH	NNP
cord-104282-90t1m430	165	8	and	and	CC
cord-104282-90t1m430	165	9	three	three	CD
cord-104282-90t1m430	165	10	truncated	truncated	JJ
cord-104282-90t1m430	165	11	proteins	protein	NNS
cord-104282-90t1m430	165	12	by	by	IN
cord-104282-90t1m430	165	13	subcellular	subcellular	JJ
cord-104282-90t1m430	165	14	fractionation	fractionation	NN
cord-104282-90t1m430	165	15	accord-	accord-	NN
cord-104282-90t1m430	166	1	Figure	figure	NN
cord-104282-90t1m430	166	2	3	3	CD
cord-104282-90t1m430	166	3	.	.	.
cord-104282-90t1m430	167	1	Effect	effect	NN
cord-104282-90t1m430	167	2	of	of	IN
cord-104282-90t1m430	167	3	sodium	sodium	NN
cord-104282-90t1m430	167	4	carbonate	carbonate	NN
cord-104282-90t1m430	167	5	treatment	treatment	NN
cord-104282-90t1m430	167	6	on	on	IN
cord-104282-90t1m430	167	7	the	the	DT
cord-104282-90t1m430	167	8	membrane	membrane	NNP
cord-104282-90t1m430	167	9	association	association	NNP
cord-104282-90t1m430	167	10	of	of	IN
cord-104282-90t1m430	167	11	wild	wild	JJ
cord-104282-90t1m430	167	12	-	-	HYPH
cord-104282-90t1m430	167	13	type	type	NN
cord-104282-90t1m430	167	14	msALDH	msaldh	NN
cord-104282-90t1m430	167	15	and	and	CC
cord-104282-90t1m430	167	16	two	two	CD
cord-104282-90t1m430	167	17	truncated	truncated	JJ
cord-104282-90t1m430	167	18	proteins	protein	NNS
cord-104282-90t1m430	167	19	.	.	.
cord-104282-90t1m430	168	1	(	(	-LRB-
cord-104282-90t1m430	168	2	,	,	,
cord-104282-90t1m430	168	3	4	4	CD
cord-104282-90t1m430	168	4	)	)	-RRB-
cord-104282-90t1m430	169	1	Each	each	DT
cord-104282-90t1m430	169	2	P3	P3	NNP
cord-104282-90t1m430	169	3	fraction	fraction	NN
cord-104282-90t1m430	169	4	was	be	VBD
cord-104282-90t1m430	169	5	treated	treat	VBN
cord-104282-90t1m430	169	6	with	with	IN
cord-104282-90t1m430	169	7	100	100	CD
cord-104282-90t1m430	169	8	mM	mm	CD
cord-104282-90t1m430	169	9	Na2CO3	na2co3	NN
cord-104282-90t1m430	169	10	for	for	IN
cord-104282-90t1m430	169	11	30	30	CD
cord-104282-90t1m430	169	12	rain	rain	NN
cord-104282-90t1m430	169	13	at	at	IN
cord-104282-90t1m430	169	14	0	0	CD
cord-104282-90t1m430	169	15	°	°	NN
cord-104282-90t1m430	169	16	C	c	NN
cord-104282-90t1m430	169	17	,	,	,
cord-104282-90t1m430	169	18	and	and	CC
cord-104282-90t1m430	169	19	centrifuged	centrifuge	VBD
cord-104282-90t1m430	169	20	at	at	IN
cord-104282-90t1m430	169	21	88,000	88,000	CD
cord-104282-90t1m430	169	22	g	g	NN
cord-104282-90t1m430	169	23	for	for	IN
cord-104282-90t1m430	169	24	80	80	CD
cord-104282-90t1m430	169	25	min	min	NN
cord-104282-90t1m430	169	26	to	to	TO
cord-104282-90t1m430	169	27	separate	separate	VB
cord-104282-90t1m430	169	28	the	the	DT
cord-104282-90t1m430	169	29	pellets	pellet	NNS
cord-104282-90t1m430	169	30	(	(	-LRB-
cord-104282-90t1m430	169	31	P	p	NN
cord-104282-90t1m430	169	32	)	)	-RRB-
cord-104282-90t1m430	169	33	from	from	IN
cord-104282-90t1m430	169	34	the	the	DT
cord-104282-90t1m430	169	35	supernatants	supernatant	NNS
cord-104282-90t1m430	169	36	(	(	-LRB-
cord-104282-90t1m430	169	37	S	S	NNP
cord-104282-90t1m430	169	38	)	)	-RRB-
cord-104282-90t1m430	169	39	.	.	.
cord-104282-90t1m430	170	1	The	the	DT
cord-104282-90t1m430	170	2	distribution	distribution	NN
cord-104282-90t1m430	170	3	of	of	IN
cord-104282-90t1m430	170	4	msALDH	msALDH	NNP
cord-104282-90t1m430	170	5	,	,	,
cord-104282-90t1m430	170	6	msALDHA481	msALDHA481	NNP
cord-104282-90t1m430	170	7	-	-	SYM
cord-104282-90t1m430	170	8	484	484	CD
cord-104282-90t1m430	170	9	,	,	,
cord-104282-90t1m430	170	10	msALDHA471	msaldha471	CD
cord-104282-90t1m430	170	11	-	-	SYM
cord-104282-90t1m430	170	12	484	484	CD
cord-104282-90t1m430	170	13	,	,	,
cord-104282-90t1m430	170	14	FP2	FP2	NNS
cord-104282-90t1m430	170	15	,	,	,
cord-104282-90t1m430	170	16	or	or	CC
cord-104282-90t1m430	170	17	PDI	PDI	NNP
cord-104282-90t1m430	170	18	in	in	IN
cord-104282-90t1m430	170	19	the	the	DT
cord-104282-90t1m430	170	20	P	p	NN
cord-104282-90t1m430	170	21	and	and	CC
cord-104282-90t1m430	170	22	S	S	NNP
cord-104282-90t1m430	170	23	fractions	fraction	NNS
cord-104282-90t1m430	170	24	was	be	VBD
cord-104282-90t1m430	170	25	assayed	assay	VBN
cord-104282-90t1m430	170	26	by	by	IN
cord-104282-90t1m430	170	27	immunoblotting	immunoblotte	VBG
cord-104282-90t1m430	170	28	.	.	.
cord-104282-90t1m430	171	1	Polyclonal	Polyclonal	NNP
cord-104282-90t1m430	171	2	anti	anti	JJ
cord-104282-90t1m430	171	3	-	-	JJ
cord-104282-90t1m430	171	4	PDI	pdi	JJ
cord-104282-90t1m430	171	5	antibody	antibody	NN
cord-104282-90t1m430	171	6	was	be	VBD
cord-104282-90t1m430	171	7	used	use	VBN
cord-104282-90t1m430	171	8	for	for	IN
cord-104282-90t1m430	171	9	immunodetection	immunodetection	NN
cord-104282-90t1m430	171	10	of	of	IN
cord-104282-90t1m430	171	11	endogenous	endogenous	JJ
cord-104282-90t1m430	171	12	PDI	PDI	NNP
cord-104282-90t1m430	171	13	.	.	.
cord-104282-90t1m430	172	1	(	(	-LRB-
cord-104282-90t1m430	172	2	B	b	NN
cord-104282-90t1m430	172	3	)	)	-RRB-
cord-104282-90t1m430	173	1	The	the	DT
cord-104282-90t1m430	173	2	relative	relative	JJ
cord-104282-90t1m430	173	3	level	level	NN
cord-104282-90t1m430	173	4	of	of	IN
cord-104282-90t1m430	173	5	each	each	DT
cord-104282-90t1m430	173	6	protein	protein	NN
cord-104282-90t1m430	173	7	in	in	IN
cord-104282-90t1m430	173	8	the	the	DT
cord-104282-90t1m430	173	9	P	P	NNP
cord-104282-90t1m430	173	10	and	and	CC
cord-104282-90t1m430	173	11	S	S	NNP
cord-104282-90t1m430	173	12	fractions	fraction	NNS
cord-104282-90t1m430	173	13	was	be	VBD
cord-104282-90t1m430	173	14	quantitated	quantitate	VBN
cord-104282-90t1m430	173	15	by	by	IN
cord-104282-90t1m430	173	16	a	a	DT
cord-104282-90t1m430	173	17	scanning	scanning	NN
cord-104282-90t1m430	173	18	densitometer	densitometer	NN
cord-104282-90t1m430	173	19	.	.	.
cord-104282-90t1m430	174	1	In	in	IN
cord-104282-90t1m430	174	2	calculating	calculate	VBG
cord-104282-90t1m430	174	3	portions	portion	NNS
cord-104282-90t1m430	174	4	recovered	recover	VBN
cord-104282-90t1m430	174	5	in	in	IN
cord-104282-90t1m430	174	6	the	the	DT
cord-104282-90t1m430	174	7	P	p	NN
cord-104282-90t1m430	174	8	fraction	fraction	NN
cord-104282-90t1m430	174	9	,	,	,
cord-104282-90t1m430	174	10	the	the	DT
cord-104282-90t1m430	174	11	total	total	JJ
cord-104282-90t1m430	174	12	level	level	NN
cord-104282-90t1m430	174	13	of	of	IN
cord-104282-90t1m430	174	14	each	each	DT
cord-104282-90t1m430	174	15	protein	protein	NN
cord-104282-90t1m430	174	16	recovered	recover	VBD
cord-104282-90t1m430	174	17	in	in	IN
cord-104282-90t1m430	174	18	the	the	DT
cord-104282-90t1m430	174	19	P	p	NN
cord-104282-90t1m430	174	20	and	and	CC
cord-104282-90t1m430	174	21	S	S	NNP
cord-104282-90t1m430	174	22	fractions	fraction	NNS
cord-104282-90t1m430	174	23	was	be	VBD
cord-104282-90t1m430	174	24	defined	define	VBN
cord-104282-90t1m430	174	25	as	as	IN
cord-104282-90t1m430	174	26	100	100	CD
cord-104282-90t1m430	174	27	%	%	NN
cord-104282-90t1m430	174	28	.	.	.
cord-104282-90t1m430	175	1	The	the	DT
cord-104282-90t1m430	175	2	bars	bar	NNS
cord-104282-90t1m430	175	3	show	show	VBP
cord-104282-90t1m430	175	4	the	the	DT
cord-104282-90t1m430	175	5	mean	mean	NN
cord-104282-90t1m430	175	6	+	+	CC
cord-104282-90t1m430	175	7	SD	sd	NN
cord-104282-90t1m430	175	8	(	(	-LRB-
cord-104282-90t1m430	175	9	n	n	NN
cord-104282-90t1m430	175	10	=	=	SYM
cord-104282-90t1m430	175	11	3	3	CD
cord-104282-90t1m430	175	12	)	)	-RRB-
cord-104282-90t1m430	175	13	.	.	.
cord-104282-90t1m430	176	1	ing	e	VBG
cord-104282-90t1m430	176	2	to	to	IN
cord-104282-90t1m430	176	3	the	the	DT
cord-104282-90t1m430	176	4	method	method	NN
cord-104282-90t1m430	176	5	of	of	IN
cord-104282-90t1m430	176	6	Clark	Clark	NNP
cord-104282-90t1m430	176	7	and	and	CC
cord-104282-90t1m430	176	8	Waterman	Waterman	NNP
cord-104282-90t1m430	176	9	(	(	-LRB-
cord-104282-90t1m430	176	10	10	10	CD
cord-104282-90t1m430	176	11	)	)	-RRB-
cord-104282-90t1m430	176	12	.	.	.
cord-104282-90t1m430	177	1	The	the	DT
cord-104282-90t1m430	177	2	separation	separation	NN
cord-104282-90t1m430	177	3	of	of	IN
cord-104282-90t1m430	177	4	ER	ER	NNP
cord-104282-90t1m430	177	5	membranes	membrane	NNS
cord-104282-90t1m430	177	6	from	from	IN
cord-104282-90t1m430	177	7	mitochondria	mitochondrion	NNS
cord-104282-90t1m430	177	8	was	be	VBD
cord-104282-90t1m430	177	9	checked	check	VBN
cord-104282-90t1m430	177	10	by	by	IN
cord-104282-90t1m430	177	11	assaying	assay	VBG
cord-104282-90t1m430	177	12	two	two	CD
cord-104282-90t1m430	177	13	typical	typical	JJ
cord-104282-90t1m430	177	14	marker	marker	NN
cord-104282-90t1m430	177	15	enzymes	enzyme	NNS
cord-104282-90t1m430	177	16	,	,	,
cord-104282-90t1m430	177	17	FP2	FP2	NNS
cord-104282-90t1m430	177	18	(	(	-LRB-
cord-104282-90t1m430	177	19	NADPH	nadph	NN
cord-104282-90t1m430	177	20	-	-	HYPH
cord-104282-90t1m430	177	21	cytochrome	cytochrome	NN
cord-104282-90t1m430	177	22	P-450	p-450	NN
cord-104282-90t1m430	177	23	reductase	reductase	NN
cord-104282-90t1m430	177	24	)	)	-RRB-
cord-104282-90t1m430	177	25	,	,	,
cord-104282-90t1m430	177	26	which	which	WDT
cord-104282-90t1m430	177	27	has	have	VBZ
cord-104282-90t1m430	177	28	been	be	VBN
cord-104282-90t1m430	177	29	shown	show	VBN
cord-104282-90t1m430	177	30	to	to	TO
cord-104282-90t1m430	177	31	be	be	VB
cord-104282-90t1m430	177	32	an	an	DT
cord-104282-90t1m430	177	33	integral	integral	JJ
cord-104282-90t1m430	177	34	ER	ER	NNP
cord-104282-90t1m430	177	35	membrane	membrane	NN
cord-104282-90t1m430	177	36	protein	protein	NN
cord-104282-90t1m430	177	37	(	(	-LRB-
cord-104282-90t1m430	177	38	22	22	CD
cord-104282-90t1m430	177	39	)	)	-RRB-
cord-104282-90t1m430	177	40	,	,	,
cord-104282-90t1m430	177	41	and	and	CC
cord-104282-90t1m430	177	42	a	a	DT
cord-104282-90t1m430	177	43	mitochondrial	mitochondrial	JJ
cord-104282-90t1m430	177	44	marker	marker	NN
cord-104282-90t1m430	177	45	succinate	succinate	NN
cord-104282-90t1m430	177	46	-	-	HYPH
cord-104282-90t1m430	177	47	cytochrome	cytochrome	NN
cord-104282-90t1m430	177	48	c	c	NN
cord-104282-90t1m430	177	49	reductase	reductase	NN
cord-104282-90t1m430	177	50	.	.	.
cord-104282-90t1m430	178	1	As	as	IN
cord-104282-90t1m430	178	2	shown	show	VBN
cord-104282-90t1m430	178	3	in	in	IN
cord-104282-90t1m430	178	4	Table	table	NN
cord-104282-90t1m430	179	1	I	-PRON-	PRP
cord-104282-90t1m430	179	2	,	,	,
cord-104282-90t1m430	179	3	FP2	fp2	NN
cord-104282-90t1m430	179	4	and	and	CC
cord-104282-90t1m430	179	5	succinate	succinate	NN
cord-104282-90t1m430	179	6	-	-	HYPH
cord-104282-90t1m430	179	7	cytochrome	cytochrome	JJ
cord-104282-90t1m430	179	8	c	c	NN
cord-104282-90t1m430	179	9	reductase	reductase	NN
cord-104282-90t1m430	179	10	were	be	VBD
cord-104282-90t1m430	179	11	enriched	enrich	VBN
cord-104282-90t1m430	179	12	in	in	IN
cord-104282-90t1m430	179	13	the	the	DT
cord-104282-90t1m430	179	14	P3	P3	NNP
cord-104282-90t1m430	179	15	fraction	fraction	NN
cord-104282-90t1m430	179	16	and	and	CC
cord-104282-90t1m430	179	17	the	the	DT
cord-104282-90t1m430	179	18	P2	P2	NNP
cord-104282-90t1m430	179	19	fraction	fraction	NN
cord-104282-90t1m430	179	20	,	,	,
cord-104282-90t1m430	179	21	respectively	respectively	RB
cord-104282-90t1m430	179	22	.	.	.
cord-104282-90t1m430	180	1	In	in	IN
cord-104282-90t1m430	180	2	addition	addition	NN
cord-104282-90t1m430	180	3	,	,	,
cord-104282-90t1m430	180	4	when	when	WRB
cord-104282-90t1m430	180	5	equal	equal	JJ
cord-104282-90t1m430	180	6	amounts	amount	NNS
cord-104282-90t1m430	180	7	of	of	IN
cord-104282-90t1m430	180	8	protein	protein	NN
cord-104282-90t1m430	180	9	were	be	VBD
cord-104282-90t1m430	180	10	immunoblotted	immunoblotte	VBN
cord-104282-90t1m430	180	11	,	,	,
cord-104282-90t1m430	180	12	the	the	DT
cord-104282-90t1m430	180	13	highest	high	JJS
cord-104282-90t1m430	180	14	levels	level	NNS
cord-104282-90t1m430	180	15	of	of	IN
cord-104282-90t1m430	180	16	FP2	FP2	NNS
cord-104282-90t1m430	180	17	and	and	CC
cord-104282-90t1m430	180	18	mitochondria	mitochondrion	NNS
cord-104282-90t1m430	180	19	Rieske	Rieske	NNP
cord-104282-90t1m430	180	20	iron	iron	NN
cord-104282-90t1m430	180	21	-	-	HYPH
cord-104282-90t1m430	180	22	sulfur	sulfur	NN
cord-104282-90t1m430	180	23	protein	protein	NN
cord-104282-90t1m430	180	24	were	be	VBD
cord-104282-90t1m430	180	25	found	find	VBN
cord-104282-90t1m430	180	26	inthe	inthe	DT
cord-104282-90t1m430	180	27	P3	P3	NNP
cord-104282-90t1m430	180	28	fraction	fraction	NN
cord-104282-90t1m430	180	29	and	and	CC
cord-104282-90t1m430	180	30	the	the	DT
cord-104282-90t1m430	180	31	P2	P2	NNP
cord-104282-90t1m430	180	32	fraction	fraction	NN
cord-104282-90t1m430	180	33	,	,	,
cord-104282-90t1m430	180	34	respectively	respectively	RB
cord-104282-90t1m430	180	35	(	(	-LRB-
cord-104282-90t1m430	180	36	Fig	fig	NN
cord-104282-90t1m430	180	37	.	.	.
cord-104282-90t1m430	180	38	2	2	CD
cord-104282-90t1m430	180	39	B	b	NN
cord-104282-90t1m430	180	40	)	)	-RRB-
cord-104282-90t1m430	180	41	.	.	.
cord-104282-90t1m430	181	1	The	the	DT
cord-104282-90t1m430	181	2	percent	percent	NN
cord-104282-90t1m430	181	3	distribution	distribution	NN
cord-104282-90t1m430	181	4	of	of	IN
cord-104282-90t1m430	181	5	the	the	DT
cord-104282-90t1m430	181	6	two	two	CD
cord-104282-90t1m430	181	7	proteins	protein	NNS
cord-104282-90t1m430	181	8	in	in	IN
cord-104282-90t1m430	181	9	each	each	DT
cord-104282-90t1m430	181	10	subcellular	subcellular	JJ
cord-104282-90t1m430	181	11	fraction	fraction	NN
cord-104282-90t1m430	181	12	also	also	RB
cord-104282-90t1m430	181	13	confirmed	confirm	VBD
cord-104282-90t1m430	181	14	a	a	DT
cord-104282-90t1m430	181	15	good	good	JJ
cord-104282-90t1m430	181	16	separation	separation	NN
cord-104282-90t1m430	181	17	of	of	IN
cord-104282-90t1m430	181	18	ER	ER	NNP
cord-104282-90t1m430	181	19	membranes	membrane	NNS
cord-104282-90t1m430	181	20	from	from	IN
cord-104282-90t1m430	181	21	mitochondria	mitochondrion	NNS
cord-104282-90t1m430	181	22	(	(	-LRB-
cord-104282-90t1m430	181	23	Fig	fig	NN
cord-104282-90t1m430	181	24	.	.	NNP
cord-104282-90t1m430	181	25	2	2	CD
cord-104282-90t1m430	181	26	C	C	NNP
cord-104282-90t1m430	181	27	)	)	-RRB-
cord-104282-90t1m430	181	28	.	.	.
cord-104282-90t1m430	182	1	The	the	DT
cord-104282-90t1m430	182	2	subceUular	subceuular	JJ
cord-104282-90t1m430	182	3	distribution	distribution	NN
cord-104282-90t1m430	182	4	of	of	IN
cord-104282-90t1m430	182	5	msALDH	msALDH	NNP
cord-104282-90t1m430	182	6	and	and	CC
cord-104282-90t1m430	182	7	msALDHA481	msALDHA481	NNP
cord-104282-90t1m430	182	8	-	-	HYPH
cord-104282-90t1m430	182	9	484	484	CD
cord-104282-90t1m430	182	10	was	be	VBD
cord-104282-90t1m430	182	11	almost	almost	RB
cord-104282-90t1m430	182	12	identical	identical	JJ
cord-104282-90t1m430	182	13	to	to	IN
cord-104282-90t1m430	182	14	that	that	DT
cord-104282-90t1m430	182	15	of	of	IN
cord-104282-90t1m430	182	16	FP2	FP2	NNS
cord-104282-90t1m430	182	17	(	(	-LRB-
cord-104282-90t1m430	182	18	Fig	fig	NN
cord-104282-90t1m430	182	19	.	.	.
cord-104282-90t1m430	182	20	 	 	_SP
cord-104282-90t1m430	183	1	2	2	LS
cord-104282-90t1m430	183	2	,	,	,
cord-104282-90t1m430	183	3	B	b	NN
cord-104282-90t1m430	183	4	and	and	CC
cord-104282-90t1m430	183	5	C	C	NNP
cord-104282-90t1m430	183	6	)	)	-RRB-
cord-104282-90t1m430	183	7	,	,	.
cord-104282-90t1m430	184	1	suggesting	suggest	VBG
cord-104282-90t1m430	184	2	the	the	DT
cord-104282-90t1m430	184	3	ER	ER	NNP
cord-104282-90t1m430	184	4	localization	localization	NN
cord-104282-90t1m430	184	5	of	of	IN
cord-104282-90t1m430	184	6	these	these	DT
cord-104282-90t1m430	184	7	proteins	protein	NNS
cord-104282-90t1m430	184	8	in	in	IN
cord-104282-90t1m430	184	9	transfected	transfecte	VBN
cord-104282-90t1m430	184	10	COS-1	COS-1	NNP
cord-104282-90t1m430	184	11	cells	cell	NNS
cord-104282-90t1m430	184	12	.	.	.
cord-104282-90t1m430	185	1	On	on	IN
cord-104282-90t1m430	185	2	the	the	DT
cord-104282-90t1m430	185	3	other	other	JJ
cord-104282-90t1m430	185	4	hand	hand	NN
cord-104282-90t1m430	185	5	,	,	,
cord-104282-90t1m430	185	6	msALDH	msALDH	NNP
cord-104282-90t1m430	185	7	A450	A450	NNP
cord-104282-90t1m430	185	8	-	-	HYPH
cord-104282-90t1m430	185	9	484	484	CD
cord-104282-90t1m430	185	10	was	be	VBD
cord-104282-90t1m430	185	11	found	find	VBN
cord-104282-90t1m430	185	12	exclusively	exclusively	RB
cord-104282-90t1m430	185	13	in	in	IN
cord-104282-90t1m430	185	14	the	the	DT
cord-104282-90t1m430	185	15	$	$	$
cord-104282-90t1m430	185	16	3	3	CD
cord-104282-90t1m430	185	17	fraction	fraction	NN
cord-104282-90t1m430	185	18	.	.	.
cord-104282-90t1m430	186	1	This	this	DT
cord-104282-90t1m430	186	2	result	result	NN
cord-104282-90t1m430	186	3	indicated	indicate	VBD
cord-104282-90t1m430	186	4	that	that	IN
cord-104282-90t1m430	186	5	the	the	DT
cord-104282-90t1m430	186	6	mutant	mutant	JJ
cord-104282-90t1m430	186	7	protein	protein	NN
cord-104282-90t1m430	186	8	lacking	lack	VBG
cord-104282-90t1m430	186	9	the	the	DT
cord-104282-90t1m430	186	10	last	last	JJ
cord-104282-90t1m430	186	11	35	35	CD
cord-104282-90t1m430	186	12	amino	amino	NNS
cord-104282-90t1m430	186	13	acid8	acid8	NN
cord-104282-90t1m430	186	14	of	of	IN
cord-104282-90t1m430	186	15	msALDH	msALDH	NNP
cord-104282-90t1m430	186	16	was	be	VBD
cord-104282-90t1m430	186	17	no	no	RB
cord-104282-90t1m430	186	18	longer	long	RBR
cord-104282-90t1m430	186	19	associated	associate	VBN
cord-104282-90t1m430	186	20	with	with	IN
cord-104282-90t1m430	186	21	intracellular	intracellular	JJ
cord-104282-90t1m430	186	22	membranes	membrane	NNS
cord-104282-90t1m430	186	23	.	.	.
cord-104282-90t1m430	187	1	Curiously	curiously	RB
cord-104282-90t1m430	187	2	,	,	,
cord-104282-90t1m430	187	3	msALDHA471	msALDHA471	:
cord-104282-90t1m430	187	4	-	-	HYPH
cord-104282-90t1m430	187	5	484	484	CD
cord-104282-90t1m430	187	6	was	be	VBD
cord-104282-90t1m430	187	7	recovered	recover	VBN
cord-104282-90t1m430	187	8	not	not	RB
cord-104282-90t1m430	187	9	only	only	RB
cord-104282-90t1m430	187	10	in	in	IN
cord-104282-90t1m430	187	11	the	the	DT
cord-104282-90t1m430	187	12	$	$	$
cord-104282-90t1m430	187	13	3	3	CD
cord-104282-90t1m430	187	14	fraction	fraction	NN
cord-104282-90t1m430	187	15	,	,	,
cord-104282-90t1m430	187	16	but	but	CC
cord-104282-90t1m430	187	17	also	also	RB
cord-104282-90t1m430	187	18	in	in	IN
cord-104282-90t1m430	187	19	the	the	DT
cord-104282-90t1m430	187	20	P3	P3	NNP
cord-104282-90t1m430	187	21	fraction	fraction	NN
cord-104282-90t1m430	187	22	,	,	,
cord-104282-90t1m430	187	23	showing	show	VBG
cord-104282-90t1m430	187	24	an	an	DT
cord-104282-90t1m430	187	25	intermediate	intermediate	JJ
cord-104282-90t1m430	187	26	distribution	distribution	NN
cord-104282-90t1m430	187	27	between	between	IN
cord-104282-90t1m430	187	28	msALDH	msaldh	NN
cord-104282-90t1m430	187	29	and	and	CC
cord-104282-90t1m430	187	30	msALDHA450	msaldha450	NN
cord-104282-90t1m430	187	31	-	-	HYPH
cord-104282-90t1m430	187	32	484	484	CD
cord-104282-90t1m430	187	33	.	.	.
cord-104282-90t1m430	188	1	We	-PRON-	PRP
cord-104282-90t1m430	188	2	explored	explore	VBD
cord-104282-90t1m430	188	3	the	the	DT
cord-104282-90t1m430	188	4	nature	nature	NN
cord-104282-90t1m430	188	5	of	of	IN
cord-104282-90t1m430	188	6	the	the	DT
cord-104282-90t1m430	188	7	association	association	NN
cord-104282-90t1m430	188	8	of	of	IN
cord-104282-90t1m430	188	9	the	the	DT
cord-104282-90t1m430	188	10	expressed	express	VBN
cord-104282-90t1m430	188	11	proteins	protein	NNS
cord-104282-90t1m430	188	12	with	with	IN
cord-104282-90t1m430	188	13	microsomal	microsomal	NNP
cord-104282-90t1m430	188	14	membranes	membrane	NNS
cord-104282-90t1m430	188	15	.	.	.
cord-104282-90t1m430	189	1	Upon	upon	IN
cord-104282-90t1m430	189	2	treatment	treatment	NN
cord-104282-90t1m430	189	3	of	of	IN
cord-104282-90t1m430	189	4	the	the	DT
cord-104282-90t1m430	189	5	P3	P3	NNP
cord-104282-90t1m430	189	6	fraction	fraction	NN
cord-104282-90t1m430	189	7	with	with	IN
cord-104282-90t1m430	189	8	100	100	CD
cord-104282-90t1m430	189	9	mM	mm	CD
cord-104282-90t1m430	189	10	Na2CO3	Na2CO3	NNP
cord-104282-90t1m430	189	11	(	(	-LRB-
cord-104282-90t1m430	189	12	pH	pH	NNP
cord-104282-90t1m430	189	13	11.5	11.5	CD
cord-104282-90t1m430	189	14	)	)	-RRB-
cord-104282-90t1m430	189	15	(	(	-LRB-
cord-104282-90t1m430	189	16	13	13	CD
cord-104282-90t1m430	189	17	)	)	-RRB-
cord-104282-90t1m430	189	18	,	,	,
cord-104282-90t1m430	189	19	FP2	FP2	NNP
cord-104282-90t1m430	189	20	(	(	-LRB-
cord-104282-90t1m430	189	21	the	the	DT
cord-104282-90t1m430	189	22	integral	integral	JJ
cord-104282-90t1m430	189	23	ER	ER	NNP
cord-104282-90t1m430	189	24	membrane	membrane	NN
cord-104282-90t1m430	189	25	protein	protein	NN
cord-104282-90t1m430	189	26	)	)	-RRB-
cord-104282-90t1m430	189	27	remained	remain	VBD
cord-104282-90t1m430	189	28	attached	attach	VBN
cord-104282-90t1m430	189	29	to	to	IN
cord-104282-90t1m430	189	30	microsomal	microsomal	NNP
cord-104282-90t1m430	189	31	membranes	membrane	NNS
cord-104282-90t1m430	189	32	as	as	IN
cord-104282-90t1m430	189	33	judged	judge	VBN
cord-104282-90t1m430	189	34	by	by	IN
cord-104282-90t1m430	189	35	immunoblotting	immunoblotte	VBG
cord-104282-90t1m430	189	36	(	(	-LRB-
cord-104282-90t1m430	190	1	Fig	Fig	NNP
cord-104282-90t1m430	190	2	.	.	.
cord-104282-90t1m430	190	3	 	 	_SP
cord-104282-90t1m430	190	4	3	3	CD
cord-104282-90t1m430	190	5	,	,	,
cord-104282-90t1m430	190	6	A	A	NNP
cord-104282-90t1m430	190	7	and	and	CC
cord-104282-90t1m430	190	8	B	b	NN
cord-104282-90t1m430	190	9	)	)	-RRB-
cord-104282-90t1m430	190	10	.	.	.
cord-104282-90t1m430	191	1	Wild	wild	JJ
cord-104282-90t1m430	191	2	-	-	HYPH
cord-104282-90t1m430	191	3	type	type	NN
cord-104282-90t1m430	192	1	msALDH	msALDH	NNP
cord-104282-90t1m430	192	2	and	and	CC
cord-104282-90t1m430	192	3	msALDI	msALDI	NNS
cord-104282-90t1m430	192	4	-	-	:
cord-104282-90t1m430	192	5	I~481	i~481	NN
cord-104282-90t1m430	192	6	-	-	HYPH
cord-104282-90t1m430	192	7	484	484	CD
cord-104282-90t1m430	192	8	were	be	VBD
cord-104282-90t1m430	192	9	also	also	RB
cord-104282-90t1m430	192	10	resistant	resistant	JJ
cord-104282-90t1m430	192	11	to	to	IN
cord-104282-90t1m430	192	12	alkali	alkali	JJ
cord-104282-90t1m430	192	13	extraction	extraction	NN
cord-104282-90t1m430	192	14	,	,	,
cord-104282-90t1m430	193	1	although	although	IN
cord-104282-90t1m430	193	2	,	,	,
cord-104282-90t1m430	193	3	x,35	x,35	VBZ
cord-104282-90t1m430	193	4	%	%	NN
cord-104282-90t1m430	193	5	of	of	IN
cord-104282-90t1m430	193	6	msALDHA481	msALDHA481	NNP
cord-104282-90t1m430	193	7	-	-	SYM
cord-104282-90t1m430	193	8	484	484	CD
cord-104282-90t1m430	193	9	was	be	VBD
cord-104282-90t1m430	193	10	released	release	VBN
cord-104282-90t1m430	193	11	.	.	.
cord-104282-90t1m430	194	1	Under	under	IN
cord-104282-90t1m430	194	2	the	the	DT
cord-104282-90t1m430	194	3	same	same	JJ
cord-104282-90t1m430	194	4	conditions	condition	NNS
cord-104282-90t1m430	194	5	,	,	,
cord-104282-90t1m430	194	6	>	>	XX
cord-104282-90t1m430	194	7	80	80	CD
cord-104282-90t1m430	194	8	%	%	NN
cord-104282-90t1m430	194	9	of	of	IN
cord-104282-90t1m430	194	10	msALDHA471	msaldha471	CD
cord-104282-90t1m430	194	11	-	-	SYM
cord-104282-90t1m430	194	12	484	484	CD
cord-104282-90t1m430	194	13	and	and	CC
cord-104282-90t1m430	194	14	PDI	PDI	NNP
cord-104282-90t1m430	194	15	(	(	-LRB-
cord-104282-90t1m430	194	16	a	a	DT
cord-104282-90t1m430	194	17	luminal	luminal	JJ
cord-104282-90t1m430	194	18	ER	er	NN
cord-104282-90t1m430	194	19	residen0	residen0	NN
cord-104282-90t1m430	194	20	were	be	VBD
cord-104282-90t1m430	194	21	released	release	VBN
cord-104282-90t1m430	194	22	,	,	,
cord-104282-90t1m430	194	23	indicating	indicate	VBG
cord-104282-90t1m430	194	24	a	a	DT
cord-104282-90t1m430	194	25	loose	loose	JJ
cord-104282-90t1m430	194	26	association	association	NN
cord-104282-90t1m430	194	27	of	of	IN
cord-104282-90t1m430	194	28	these	these	DT
cord-104282-90t1m430	194	29	proteins	protein	NNS
cord-104282-90t1m430	194	30	with	with	IN
cord-104282-90t1m430	194	31	microsomal	microsomal	NNP
cord-104282-90t1m430	194	32	membranes	membrane	NNS
cord-104282-90t1m430	194	33	.	.	.
cord-104282-90t1m430	195	1	Similar	similar	JJ
cord-104282-90t1m430	195	2	results	result	NNS
cord-104282-90t1m430	195	3	were	be	VBD
cord-104282-90t1m430	195	4	obtained	obtain	VBN
cord-104282-90t1m430	195	5	upon	upon	IN
cord-104282-90t1m430	195	6	Triton	Triton	NNP
cord-104282-90t1m430	195	7	X-114	X-114	VBZ
cord-104282-90t1m430	195	8	cloud	cloud	NN
cord-104282-90t1m430	195	9	point	point	NN
cord-104282-90t1m430	195	10	extraction	extraction	NN
cord-104282-90t1m430	195	11	(	(	-LRB-
cord-104282-90t1m430	195	12	6	6	CD
cord-104282-90t1m430	195	13	)	)	-RRB-
cord-104282-90t1m430	195	14	(	(	-LRB-
cord-104282-90t1m430	195	15	data	datum	NNS
cord-104282-90t1m430	195	16	not	not	RB
cord-104282-90t1m430	195	17	shown	show	VBN
cord-104282-90t1m430	195	18	)	)	-RRB-
cord-104282-90t1m430	195	19	.	.	.
cord-104282-90t1m430	196	1	These	these	DT
cord-104282-90t1m430	196	2	data	datum	NNS
cord-104282-90t1m430	196	3	,	,	,
cord-104282-90t1m430	196	4	together	together	RB
cord-104282-90t1m430	196	5	with	with	IN
cord-104282-90t1m430	196	6	those	those	DT
cord-104282-90t1m430	196	7	from	from	IN
cord-104282-90t1m430	196	8	subcellular	subcellular	JJ
cord-104282-90t1m430	196	9	fractionation	fractionation	NN
cord-104282-90t1m430	196	10	,	,	,
cord-104282-90t1m430	196	11	suggested	suggest	VBD
cord-104282-90t1m430	196	12	that	that	IN
cord-104282-90t1m430	196	13	the	the	DT
cord-104282-90t1m430	196	14	carboxyl	carboxyl	NN
cord-104282-90t1m430	196	15	-	-	HYPH
cord-104282-90t1m430	196	16	terminal	terminal	NN
cord-104282-90t1m430	196	17	portion	portion	NN
cord-104282-90t1m430	196	18	of	of	IN
cord-104282-90t1m430	196	19	msALDH	msALDH	NNP
cord-104282-90t1m430	196	20	including	include	VBG
cord-104282-90t1m430	196	21	the	the	DT
cord-104282-90t1m430	196	22	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	196	23	sequence	sequence	NN
cord-104282-90t1m430	196	24	(	(	-LRB-
cord-104282-90t1m430	196	25	amino	amino	NN
cord-104282-90t1m430	196	26	acid	acid	NN
cord-104282-90t1m430	196	27	464	464	CD
cord-104282-90t1m430	196	28	-	-	SYM
cord-104282-90t1m430	196	29	480	480	CD
cord-104282-90t1m430	196	30	)	)	-RRB-
cord-104282-90t1m430	196	31	was	be	VBD
cord-104282-90t1m430	196	32	necessary	necessary	JJ
cord-104282-90t1m430	196	33	for	for	IN
cord-104282-90t1m430	196	34	both	both	DT
cord-104282-90t1m430	196	35	its	-PRON-	PRP$
cord-104282-90t1m430	196	36	ER	ER	NNP
cord-104282-90t1m430	196	37	localization	localization	NN
cord-104282-90t1m430	196	38	and	and	CC
cord-104282-90t1m430	196	39	the	the	DT
cord-104282-90t1m430	196	40	tight	tight	JJ
cord-104282-90t1m430	196	41	association	association	NN
cord-104282-90t1m430	196	42	with	with	IN
cord-104282-90t1m430	196	43	the	the	DT
cord-104282-90t1m430	196	44	ER	ER	NNP
cord-104282-90t1m430	196	45	membrane	membrane	NN
cord-104282-90t1m430	196	46	.	.	.
cord-104282-90t1m430	197	1	We	-PRON-	PRP
cord-104282-90t1m430	197	2	next	next	RB
cord-104282-90t1m430	197	3	determined	determine	VBD
cord-104282-90t1m430	197	4	the	the	DT
cord-104282-90t1m430	197	5	intracellular	intracellular	JJ
cord-104282-90t1m430	197	6	localization	localization	NN
cord-104282-90t1m430	197	7	of	of	IN
cord-104282-90t1m430	197	8	msALDH	msALDH	NNP
cord-104282-90t1m430	197	9	and	and	CC
cord-104282-90t1m430	197	10	the	the	DT
cord-104282-90t1m430	197	11	truncated	truncated	JJ
cord-104282-90t1m430	197	12	proteins	protein	NNS
cord-104282-90t1m430	197	13	using	use	VBG
cord-104282-90t1m430	197	14	indirect	indirect	JJ
cord-104282-90t1m430	197	15	immunofluorescence	immunofluorescence	NN
cord-104282-90t1m430	197	16	microscopy	microscopy	NN
cord-104282-90t1m430	197	17	.	.	.
cord-104282-90t1m430	198	1	Cells	cell	NNS
cord-104282-90t1m430	198	2	transfected	transfecte	VBN
cord-104282-90t1m430	198	3	with	with	IN
cord-104282-90t1m430	198	4	cDNAs	cdna	NNS
cord-104282-90t1m430	198	5	in	in	IN
cord-104282-90t1m430	198	6	the	the	DT
cord-104282-90t1m430	198	7	pCD	pcd	XX
cord-104282-90t1m430	198	8	vector	vector	NN
cord-104282-90t1m430	198	9	were	be	VBD
cord-104282-90t1m430	198	10	fixed	fix	VBN
cord-104282-90t1m430	198	11	and	and	CC
cord-104282-90t1m430	198	12	permeabilized	permeabilized	JJ
cord-104282-90t1m430	198	13	44	44	CD
cord-104282-90t1m430	198	14	h	h	NN
cord-104282-90t1m430	198	15	after	after	IN
cord-104282-90t1m430	198	16	transfection	transfection	NN
cord-104282-90t1m430	198	17	,	,	,
cord-104282-90t1m430	198	18	and	and	CC
cord-104282-90t1m430	198	19	the	the	DT
cord-104282-90t1m430	198	20	expressed	express	VBN
cord-104282-90t1m430	198	21	proteins	protein	NNS
cord-104282-90t1m430	198	22	were	be	VBD
cord-104282-90t1m430	198	23	detected	detect	VBN
cord-104282-90t1m430	198	24	by	by	IN
cord-104282-90t1m430	198	25	incubation	incubation	NN
cord-104282-90t1m430	198	26	with	with	IN
cord-104282-90t1m430	198	27	anti	anti	JJ
cord-104282-90t1m430	198	28	-	-	JJ
cord-104282-90t1m430	198	29	msALDH	msaldh	JJ
cord-104282-90t1m430	198	30	antibody	antibody	NN
cord-104282-90t1m430	198	31	followed	follow	VBN
cord-104282-90t1m430	198	32	by	by	IN
cord-104282-90t1m430	198	33	TRITC	TRITC	NNP
cord-104282-90t1m430	198	34	-	-	HYPH
cord-104282-90t1m430	198	35	conjugated	conjugate	VBN
cord-104282-90t1m430	198	36	secondary	secondary	JJ
cord-104282-90t1m430	198	37	antibody	antibody	NN
cord-104282-90t1m430	198	38	.	.	.
cord-104282-90t1m430	199	1	As	as	IN
cord-104282-90t1m430	199	2	shown	show	VBN
cord-104282-90t1m430	199	3	in	in	IN
cord-104282-90t1m430	199	4	Fig	Fig	NNP
cord-104282-90t1m430	199	5	.	.	.
cord-104282-90t1m430	200	1	4	4	LS
cord-104282-90t1m430	200	2	A	a	DT
cord-104282-90t1m430	200	3	,	,	,
cord-104282-90t1m430	200	4	anti	anti	JJ
cord-104282-90t1m430	200	5	-	-	JJ
cord-104282-90t1m430	200	6	msALDH	msaldh	JJ
cord-104282-90t1m430	200	7	antibody	antibody	NN
cord-104282-90t1m430	200	8	stained	stain	VBD
cord-104282-90t1m430	200	9	a	a	DT
cord-104282-90t1m430	200	10	number	number	NN
cord-104282-90t1m430	200	11	of	of	IN
cord-104282-90t1m430	200	12	large	large	JJ
cord-104282-90t1m430	200	13	vesicular	vesicular	JJ
cord-104282-90t1m430	200	14	structures	structure	NNS
cord-104282-90t1m430	200	15	that	that	WDT
cord-104282-90t1m430	200	16	surrounded	surround	VBD
cord-104282-90t1m430	200	17	the	the	DT
cord-104282-90t1m430	200	18	nucleus	nucleus	NN
cord-104282-90t1m430	200	19	,	,	,
cord-104282-90t1m430	200	20	in	in	IN
cord-104282-90t1m430	200	21	addition	addition	NN
cord-104282-90t1m430	200	22	to	to	TO
cord-104282-90t1m430	200	23	diffuse	diffuse	VB
cord-104282-90t1m430	200	24	reticular	reticular	JJ
cord-104282-90t1m430	200	25	ones	one	NNS
cord-104282-90t1m430	200	26	in	in	IN
cord-104282-90t1m430	200	27	COS-1	COS-1	NNP
cord-104282-90t1m430	200	28	cells	cell	NNS
cord-104282-90t1m430	200	29	transfected	transfecte	VBN
cord-104282-90t1m430	200	30	with	with	IN
cord-104282-90t1m430	200	31	msALDH	msALDH	NNP
cord-104282-90t1m430	200	32	eDNA	edna	NN
cord-104282-90t1m430	200	33	.	.	.
cord-104282-90t1m430	201	1	No	no	DT
cord-104282-90t1m430	201	2	staining	staining	NN
cord-104282-90t1m430	201	3	was	be	VBD
cord-104282-90t1m430	201	4	seen	see	VBN
cord-104282-90t1m430	201	5	at	at	IN
cord-104282-90t1m430	201	6	the	the	DT
cord-104282-90t1m430	201	7	plasma	plasma	NN
cord-104282-90t1m430	201	8	membrane	membrane	NN
cord-104282-90t1m430	201	9	and	and	CC
cord-104282-90t1m430	201	10	in	in	IN
cord-104282-90t1m430	201	11	the	the	DT
cord-104282-90t1m430	201	12	nucleoplasm	nucleoplasm	NN
cord-104282-90t1m430	201	13	.	.	.
cord-104282-90t1m430	202	1	To	to	TO
cord-104282-90t1m430	202	2	explore	explore	VB
cord-104282-90t1m430	202	3	this	this	DT
cord-104282-90t1m430	202	4	strange	strange	JJ
cord-104282-90t1m430	202	5	structure	structure	NN
cord-104282-90t1m430	202	6	in	in	IN
cord-104282-90t1m430	202	7	more	more	JJR
cord-104282-90t1m430	202	8	detail	detail	NN
cord-104282-90t1m430	202	9	,	,	,
cord-104282-90t1m430	202	10	we	-PRON-	PRP
cord-104282-90t1m430	202	11	used	use	VBD
cord-104282-90t1m430	202	12	double	double	JJ
cord-104282-90t1m430	202	13	indirect	indirect	JJ
cord-104282-90t1m430	202	14	immunofluorescence	immunofluorescence	NN
cord-104282-90t1m430	202	15	microscopy	microscopy	NN
cord-104282-90t1m430	202	16	using	use	VBG
cord-104282-90t1m430	202	17	two	two	CD
cord-104282-90t1m430	202	18	mAbs	mAbs	NNP
cord-104282-90t1m430	202	19	or	or	CC
cord-104282-90t1m430	202	20	FITC	FITC	NNP
cord-104282-90t1m430	202	21	-	-	HYPH
cord-104282-90t1m430	202	22	conjugated	conjugate	VBN
cord-104282-90t1m430	202	23	lectin	lectin	NN
cord-104282-90t1m430	202	24	.	.	.
cord-104282-90t1m430	203	1	Expressed	express	VBN
cord-104282-90t1m430	203	2	msALDH	msALDH	NNP
cord-104282-90t1m430	203	3	was	be	VBD
cord-104282-90t1m430	203	4	found	find	VBN
cord-104282-90t1m430	203	5	to	to	IN
cord-104282-90t1m430	203	6	colocalize	colocalize	VB
cord-104282-90t1m430	203	7	with	with	IN
cord-104282-90t1m430	203	8	endogenous	endogenous	JJ
cord-104282-90t1m430	203	9	PDI	PDI	NNP
cord-104282-90t1m430	203	10	(	(	-LRB-
cord-104282-90t1m430	203	11	Fig	fig	NN
cord-104282-90t1m430	203	12	.	.	.
cord-104282-90t1m430	203	13	4	4	CD
cord-104282-90t1m430	203	14	B	b	NN
cord-104282-90t1m430	203	15	,	,	,
cord-104282-90t1m430	203	16	arrow	arrow	NN
cord-104282-90t1m430	203	17	)	)	-RRB-
cord-104282-90t1m430	203	18	,	,	,
cord-104282-90t1m430	203	19	although	although	IN
cord-104282-90t1m430	203	20	PDI	PDI	NNP
cord-104282-90t1m430	203	21	displayed	display	VBD
cord-104282-90t1m430	203	22	a	a	DT
cord-104282-90t1m430	203	23	characteristic	characteristic	JJ
cord-104282-90t1m430	203	24	reticular	reticular	JJ
cord-104282-90t1m430	203	25	staining	staining	NN
cord-104282-90t1m430	203	26	pattern	pattern	NN
cord-104282-90t1m430	203	27	in	in	IN
cord-104282-90t1m430	203	28	cells	cell	NNS
cord-104282-90t1m430	203	29	not	not	RB
cord-104282-90t1m430	203	30	expressing	express	VBG
cord-104282-90t1m430	203	31	msALDH	msALDH	NNP
cord-104282-90t1m430	204	1	(	(	-LRB-
cord-104282-90t1m430	204	2	Fig	Fig	NNP
cord-104282-90t1m430	204	3	.	.	.
cord-104282-90t1m430	204	4	4	4	CD
cord-104282-90t1m430	204	5	B	b	NN
cord-104282-90t1m430	204	6	,	,	,
cord-104282-90t1m430	204	7	arrowhead	arrowhead	NN
cord-104282-90t1m430	204	8	)	)	-RRB-
cord-104282-90t1m430	204	9	.	.	.
cord-104282-90t1m430	205	1	In	in	IN
cord-104282-90t1m430	205	2	contrast	contrast	NN
cord-104282-90t1m430	205	3	,	,	,
cord-104282-90t1m430	205	4	msALDH	msALDH	NNP
cord-104282-90t1m430	205	5	colocalized	colocalize	VBN
cord-104282-90t1m430	205	6	neither	neither	CC
cord-104282-90t1m430	205	7	with	with	IN
cord-104282-90t1m430	205	8	a	a	DT
cord-104282-90t1m430	205	9	mitochondrial	mitochondrial	NN
cord-104282-90t1m430	205	10	65-kD	65-kd	CD
cord-104282-90t1m430	205	11	protein	protein	NN
cord-104282-90t1m430	205	12	(	(	-LRB-
cord-104282-90t1m430	205	13	Fig	Fig	NNP
cord-104282-90t1m430	205	14	.	.	NNP
cord-104282-90t1m430	205	15	4	4	CD
cord-104282-90t1m430	205	16	,	,	,
cord-104282-90t1m430	205	17	C	C	NNP
cord-104282-90t1m430	205	18	and	and	CC
cord-104282-90t1m430	205	19	D	D	NNP
cord-104282-90t1m430	205	20	)	)	-RRB-
cord-104282-90t1m430	205	21	nor	nor	CC
cord-104282-90t1m430	205	22	with	with	IN
cord-104282-90t1m430	205	23	the	the	DT
cord-104282-90t1m430	205	24	perinuclear	perinuclear	NNP
cord-104282-90t1m430	205	25	Golgi	Golgi	NNP
cord-104282-90t1m430	205	26	complex	complex	NN
cord-104282-90t1m430	205	27	visualized	visualize	VBN
cord-104282-90t1m430	205	28	by	by	IN
cord-104282-90t1m430	205	29	FITC	FITC	NNP
cord-104282-90t1m430	205	30	-	-	HYPH
cord-104282-90t1m430	205	31	conjugated	conjugate	VBN
cord-104282-90t1m430	205	32	wheat	wheat	NN
cord-104282-90t1m430	205	33	germ	germ	NN
cord-104282-90t1m430	205	34	agglutinin	agglutinin	NNS
cord-104282-90t1m430	205	35	(	(	-LRB-
cord-104282-90t1m430	205	36	Fig	Fig	NNP
cord-104282-90t1m430	205	37	.	.	NNP
cord-104282-90t1m430	205	38	4	4	CD
cord-104282-90t1m430	205	39	,	,	,
cord-104282-90t1m430	205	40	E	E	NNP
cord-104282-90t1m430	205	41	and	and	CC
cord-104282-90t1m430	205	42	F	F	NNP
cord-104282-90t1m430	205	43	)	)	-RRB-
cord-104282-90t1m430	205	44	.	.	.
cord-104282-90t1m430	206	1	These	these	DT
cord-104282-90t1m430	206	2	results	result	NNS
cord-104282-90t1m430	206	3	strongly	strongly	RB
cord-104282-90t1m430	206	4	suggest	suggest	VBP
cord-104282-90t1m430	206	5	the	the	DT
cord-104282-90t1m430	206	6	ER	ER	NNP
cord-104282-90t1m430	206	7	localization	localization	NN
cord-104282-90t1m430	206	8	of	of	IN
cord-104282-90t1m430	206	9	msALDH	msALDH	NNP
cord-104282-90t1m430	206	10	and	and	CC
cord-104282-90t1m430	206	11	the	the	DT
cord-104282-90t1m430	206	12	drastic	drastic	JJ
cord-104282-90t1m430	206	13	morphological	morphological	JJ
cord-104282-90t1m430	206	14	change	change	NN
cord-104282-90t1m430	206	15	of	of	IN
cord-104282-90t1m430	206	16	the	the	DT
cord-104282-90t1m430	206	17	ER	ER	NNP
cord-104282-90t1m430	206	18	by	by	IN
cord-104282-90t1m430	206	19	expression	expression	NN
cord-104282-90t1m430	206	20	of	of	IN
cord-104282-90t1m430	206	21	msALDH	msALDH	NNP
cord-104282-90t1m430	206	22	.	.	.
cord-104282-90t1m430	207	1	A	a	DT
cord-104282-90t1m430	207	2	similar	similar	JJ
cord-104282-90t1m430	207	3	ER	er	NN
cord-104282-90t1m430	207	4	staining	staining	NN
cord-104282-90t1m430	207	5	pattern	pattern	NN
cord-104282-90t1m430	207	6	was	be	VBD
cord-104282-90t1m430	207	7	observed	observe	VBN
cord-104282-90t1m430	207	8	for	for	IN
cord-104282-90t1m430	207	9	msALDHA481	msALDHA481	NNP
cord-104282-90t1m430	207	10	-	-	SYM
cord-104282-90t1m430	207	11	484	484	CD
cord-104282-90t1m430	208	1	(	(	-LRB-
cord-104282-90t1m430	208	2	Fig	Fig	NNP
cord-104282-90t1m430	208	3	.	.	NNP
cord-104282-90t1m430	208	4	4	4	CD
cord-104282-90t1m430	208	5	,	,	,
cord-104282-90t1m430	208	6	G	G	NNP
cord-104282-90t1m430	208	7	and	and	CC
cord-104282-90t1m430	208	8	H	H	NNP
cord-104282-90t1m430	208	9	)	)	-RRB-
cord-104282-90t1m430	208	10	,	,	,
cord-104282-90t1m430	208	11	whereas	whereas	IN
cord-104282-90t1m430	208	12	both	both	DT
cord-104282-90t1m430	208	13	msALDHA471	msaldha471	CD
cord-104282-90t1m430	208	14	-	-	SYM
cord-104282-90t1m430	208	15	484	484	CD
cord-104282-90t1m430	208	16	and	and	CC
cord-104282-90t1m430	208	17	msALDHA450	msaldha450	NN
cord-104282-90t1m430	208	18	-	-	HYPH
cord-104282-90t1m430	208	19	484	484	CD
cord-104282-90t1m430	208	20	were	be	VBD
cord-104282-90t1m430	208	21	found	find	VBN
cord-104282-90t1m430	208	22	distributed	distribute	VBN
cord-104282-90t1m430	208	23	diffusely	diffusely	RB
cord-104282-90t1m430	208	24	throughout	throughout	IN
cord-104282-90t1m430	208	25	the	the	DT
cord-104282-90t1m430	208	26	cytoplasm	cytoplasm	NN
cord-104282-90t1m430	208	27	,	,	,
cord-104282-90t1m430	208	28	and	and	CC
cord-104282-90t1m430	208	29	they	-PRON-	PRP
cord-104282-90t1m430	208	30	did	do	VBD
cord-104282-90t1m430	208	31	not	not	RB
cord-104282-90t1m430	208	32	colocalize	colocalize	VB
cord-104282-90t1m430	208	33	with	with	IN
cord-104282-90t1m430	208	34	PDI	PDI	NNP
cord-104282-90t1m430	208	35	(	(	-LRB-
cord-104282-90t1m430	208	36	Fig	fig	NN
cord-104282-90t1m430	208	37	.	.	.
cord-104282-90t1m430	209	1	4	4	LS
cord-104282-90t1m430	209	2	,	,	,
cord-104282-90t1m430	209	3	I	I	NNP
cord-104282-90t1m430	209	4	-	-	HYPH
cord-104282-90t1m430	209	5	L	L	NNP
cord-104282-90t1m430	209	6	)	)	-RRB-
cord-104282-90t1m430	209	7	.	.	.
cord-104282-90t1m430	210	1	In	in	IN
cord-104282-90t1m430	210	2	addition	addition	NN
cord-104282-90t1m430	210	3	,	,	,
cord-104282-90t1m430	210	4	a	a	DT
cord-104282-90t1m430	210	5	significant	significant	JJ
cord-104282-90t1m430	210	6	amount	amount	NN
cord-104282-90t1m430	210	7	of	of	IN
cord-104282-90t1m430	210	8	msALDHA450	msALDHA450	NNP
cord-104282-90t1m430	210	9	-	-	HYPH
cord-104282-90t1m430	210	10	484	484	CD
cord-104282-90t1m430	210	11	appeared	appear	VBD
cord-104282-90t1m430	210	12	to	to	TO
cord-104282-90t1m430	210	13	enter	enter	VB
cord-104282-90t1m430	210	14	the	the	DT
cord-104282-90t1m430	210	15	nucleus	nucleus	NN
cord-104282-90t1m430	211	1	(	(	-LRB-
cord-104282-90t1m430	211	2	Fig	Fig	NNP
cord-104282-90t1m430	211	3	.	.	.
cord-104282-90t1m430	211	4	4	4	CD
cord-104282-90t1m430	211	5	K	K	NNP
cord-104282-90t1m430	211	6	)	)	-RRB-
cord-104282-90t1m430	211	7	.	.	.
cord-104282-90t1m430	212	1	These	these	DT
cord-104282-90t1m430	212	2	immunolocalization	immunolocalization	NN
cord-104282-90t1m430	212	3	data	datum	NNS
cord-104282-90t1m430	212	4	were	be	VBD
cord-104282-90t1m430	212	5	consistent	consistent	JJ
cord-104282-90t1m430	212	6	with	with	IN
cord-104282-90t1m430	212	7	those	those	DT
cord-104282-90t1m430	212	8	from	from	IN
cord-104282-90t1m430	212	9	subcellular	subcellular	JJ
cord-104282-90t1m430	212	10	fractionation	fractionation	NN
cord-104282-90t1m430	212	11	,	,	,
cord-104282-90t1m430	212	12	suggesting	suggest	VBG
cord-104282-90t1m430	212	13	strongly	strongly	RB
cord-104282-90t1m430	212	14	the	the	DT
cord-104282-90t1m430	212	15	important	important	JJ
cord-104282-90t1m430	212	16	role	role	NN
cord-104282-90t1m430	212	17	of	of	IN
cord-104282-90t1m430	212	18	the	the	DT
cord-104282-90t1m430	212	19	carboxyl	carboxyl	NN
cord-104282-90t1m430	212	20	-	-	HYPH
cord-104282-90t1m430	212	21	terminal	terminal	NN
cord-104282-90t1m430	212	22	portion	portion	NN
cord-104282-90t1m430	212	23	of	of	IN
cord-104282-90t1m430	212	24	msALDH	msALDH	NNP
cord-104282-90t1m430	212	25	in	in	IN
cord-104282-90t1m430	212	26	its	-PRON-	PRP$
cord-104282-90t1m430	212	27	ER	ER	NNP
cord-104282-90t1m430	212	28	localization	localization	NN
cord-104282-90t1m430	212	29	.	.	.
cord-104282-90t1m430	213	1	We	-PRON-	PRP
cord-104282-90t1m430	213	2	focused	focus	VBD
cord-104282-90t1m430	213	3	our	-PRON-	PRP$
cord-104282-90t1m430	213	4	attention	attention	NN
cord-104282-90t1m430	213	5	on	on	IN
cord-104282-90t1m430	213	6	the	the	DT
cord-104282-90t1m430	213	7	role	role	NN
cord-104282-90t1m430	213	8	that	that	WDT
cord-104282-90t1m430	213	9	the	the	DT
cord-104282-90t1m430	213	10	carboxylterminal	carboxylterminal	JJ
cord-104282-90t1m430	213	11	portion	portion	NN
cord-104282-90t1m430	213	12	of	of	IN
cord-104282-90t1m430	213	13	msALDH	msALDH	NNP
cord-104282-90t1m430	213	14	might	may	MD
cord-104282-90t1m430	213	15	play	play	VB
cord-104282-90t1m430	213	16	in	in	IN
cord-104282-90t1m430	213	17	ER	ER	NNP
cord-104282-90t1m430	213	18	targeting	targeting	NN
cord-104282-90t1m430	213	19	,	,	,
cord-104282-90t1m430	213	20	and	and	CC
cord-104282-90t1m430	213	21	we	-PRON-	PRP
cord-104282-90t1m430	213	22	asked	ask	VBD
cord-104282-90t1m430	213	23	whether	whether	IN
cord-104282-90t1m430	213	24	this	this	DT
cord-104282-90t1m430	213	25	portion	portion	NN
cord-104282-90t1m430	213	26	could	could	MD
cord-104282-90t1m430	213	27	direct	direct	VB
cord-104282-90t1m430	213	28	a	a	DT
cord-104282-90t1m430	213	29	heterologous	heterologous	JJ
cord-104282-90t1m430	213	30	protein	protein	NN
cord-104282-90t1m430	213	31	to	to	IN
cord-104282-90t1m430	213	32	the	the	DT
cord-104282-90t1m430	213	33	ER	ER	NNP
cord-104282-90t1m430	213	34	membrane	membrane	NN
cord-104282-90t1m430	213	35	.	.	.
cord-104282-90t1m430	214	1	For	for	IN
cord-104282-90t1m430	214	2	this	this	DT
cord-104282-90t1m430	214	3	purpose	purpose	NN
cord-104282-90t1m430	214	4	,	,	,
cord-104282-90t1m430	214	5	we	-PRON-	PRP
cord-104282-90t1m430	214	6	constructed	construct	VBD
cord-104282-90t1m430	214	7	an	an	DT
cord-104282-90t1m430	214	8	expression	expression	NN
cord-104282-90t1m430	214	9	plasmid	plasmid	NN
cord-104282-90t1m430	214	10	shown	show	VBN
cord-104282-90t1m430	214	11	in	in	IN
cord-104282-90t1m430	214	12	Fig	Fig	NNP
cord-104282-90t1m430	214	13	.	.	.
cord-104282-90t1m430	215	1	5	5	CD
cord-104282-90t1m430	215	2	A.	a.	NN
cord-104282-90t1m430	216	1	The	the	DT
cord-104282-90t1m430	216	2	control	control	NN
cord-104282-90t1m430	216	3	vector	vector	NN
cord-104282-90t1m430	216	4	(	(	-LRB-
cord-104282-90t1m430	216	5	pCHll0	pchll0	NN
cord-104282-90t1m430	216	6	)	)	-RRB-
cord-104282-90t1m430	216	7	contains	contain	VBZ
cord-104282-90t1m430	216	8	E.	E.	NNP
cord-104282-90t1m430	216	9	coli/3-galactosidase	coli/3-galactosidase	FW
cord-104282-90t1m430	216	10	,	,	,
cord-104282-90t1m430	216	11	which	which	WDT
cord-104282-90t1m430	216	12	The	the	DT
cord-104282-90t1m430	216	13	chimeric	chimeric	JJ
cord-104282-90t1m430	216	14	eDNA	edna	NN
cord-104282-90t1m430	216	15	fragment	fragment	NN
cord-104282-90t1m430	216	16	with	with	IN
cord-104282-90t1m430	216	17	EcoRI	ecori	NN
cord-104282-90t1m430	216	18	sites	site	NNS
cord-104282-90t1m430	216	19	shown	show	VBN
cord-104282-90t1m430	216	20	by	by	IN
cord-104282-90t1m430	216	21	the	the	DT
cord-104282-90t1m430	216	22	bar	bar	NN
cord-104282-90t1m430	216	23	was	be	VBD
cord-104282-90t1m430	216	24	amplified	amplify	VBN
cord-104282-90t1m430	216	25	by	by	IN
cord-104282-90t1m430	216	26	PCR	PCR	NNP
cord-104282-90t1m430	216	27	and	and	CC
cord-104282-90t1m430	216	28	cloned	clone	VBN
cord-104282-90t1m430	216	29	in	in	IN
cord-104282-90t1m430	216	30	-	-	HYPH
cord-104282-90t1m430	216	31	frame	frame	NN
cord-104282-90t1m430	216	32	to	to	IN
cord-104282-90t1m430	216	33	the	the	DT
cord-104282-90t1m430	216	34	unique	unique	JJ
cord-104282-90t1m430	216	35	EcoRI	ecori	NN
cord-104282-90t1m430	216	36	site	site	NN
cord-104282-90t1m430	216	37	in	in	IN
cord-104282-90t1m430	216	38	pCHll0	pCHll0	NNP
cord-104282-90t1m430	216	39	as	as	IN
cord-104282-90t1m430	216	40	described	describe	VBN
cord-104282-90t1m430	216	41	in	in	IN
cord-104282-90t1m430	216	42	Materials	material	NNS
cord-104282-90t1m430	216	43	and	and	CC
cord-104282-90t1m430	216	44	Methods	method	NNS
cord-104282-90t1m430	216	45	.	.	.
cord-104282-90t1m430	217	1	To	to	IN
cord-104282-90t1m430	217	2	the	the	DT
cord-104282-90t1m430	217	3	right	right	NN
cord-104282-90t1m430	217	4	,	,	,
cord-104282-90t1m430	217	5	the	the	DT
cord-104282-90t1m430	217	6	intracellular	intracellular	JJ
cord-104282-90t1m430	217	7	location	location	NN
cord-104282-90t1m430	217	8	of	of	IN
cord-104282-90t1m430	217	9	each	each	DT
cord-104282-90t1m430	217	10	protein	protein	NN
cord-104282-90t1m430	217	11	is	be	VBZ
cord-104282-90t1m430	217	12	noted	note	VBN
cord-104282-90t1m430	217	13	as	as	IN
cord-104282-90t1m430	217	14	E	E	NNP
cord-104282-90t1m430	217	15	for	for	IN
cord-104282-90t1m430	217	16	ER	ER	NNP
cord-104282-90t1m430	217	17	or	or	CC
cord-104282-90t1m430	217	18	C	C	NNP
cord-104282-90t1m430	217	19	for	for	IN
cord-104282-90t1m430	217	20	cytosolie	cytosolie	NNP
cord-104282-90t1m430	217	21	.	.	.
cord-104282-90t1m430	218	1	(	(	-LRB-
cord-104282-90t1m430	218	2	B	b	NN
cord-104282-90t1m430	218	3	)	)	-RRB-
cord-104282-90t1m430	218	4	COS-1	COS-1	NNP
cord-104282-90t1m430	218	5	cells	cell	NNS
cord-104282-90t1m430	218	6	were	be	VBD
cord-104282-90t1m430	218	7	transfected	transfecte	VBN
cord-104282-90t1m430	218	8	with	with	IN
cord-104282-90t1m430	218	9	pCHll0	pCHll0	NNP
cord-104282-90t1m430	218	10	(	(	-LRB-
cord-104282-90t1m430	218	11	~Gal	~Gal	NFP
cord-104282-90t1m430	218	12	)	)	-RRB-
cord-104282-90t1m430	219	1	or	or	CC
cord-104282-90t1m430	219	2	pCHll0	pCHll0	VBN
cord-104282-90t1m430	219	3	/	/	SYM
cord-104282-90t1m430	219	4	ALDH	ALDH	NNP
cord-104282-90t1m430	219	5	~3GaI	~3GaI	NNP
cord-104282-90t1m430	219	6	-	-	HYPH
cord-104282-90t1m430	219	7	ALDH	ALDH	NNP
cord-104282-90t1m430	219	8	)	)	-RRB-
cord-104282-90t1m430	219	9	,	,	,
cord-104282-90t1m430	219	10	and	and	CC
cord-104282-90t1m430	219	11	harvested	harvest	VBD
cord-104282-90t1m430	219	12	44	44	CD
cord-104282-90t1m430	219	13	h	h	NN
cord-104282-90t1m430	219	14	after	after	IN
cord-104282-90t1m430	219	15	transfection	transfection	NN
cord-104282-90t1m430	219	16	.	.	.
cord-104282-90t1m430	220	1	The	the	DT
cord-104282-90t1m430	220	2	membrane	membrane	NN
cord-104282-90t1m430	220	3	(	(	-LRB-
cord-104282-90t1m430	220	4	P	p	NN
cord-104282-90t1m430	220	5	)	)	-RRB-
cord-104282-90t1m430	220	6	and	and	CC
cord-104282-90t1m430	220	7	cytosol	cytosol	NN
cord-104282-90t1m430	220	8	(	(	-LRB-
cord-104282-90t1m430	220	9	S	S	NNP
cord-104282-90t1m430	220	10	)	)	-RRB-
cord-104282-90t1m430	220	11	fractions	fraction	NNS
cord-104282-90t1m430	220	12	were	be	VBD
cord-104282-90t1m430	220	13	prepared	prepare	VBN
cord-104282-90t1m430	220	14	by	by	IN
cord-104282-90t1m430	220	15	centrifugation	centrifugation	NN
cord-104282-90t1m430	220	16	of	of	IN
cord-104282-90t1m430	220	17	the	the	DT
cord-104282-90t1m430	220	18	postnuelear	postnuelear	JJ
cord-104282-90t1m430	220	19	fractions	fraction	NNS
cord-104282-90t1m430	220	20	at	at	IN
cord-104282-90t1m430	220	21	88,000	88,000	CD
cord-104282-90t1m430	220	22	g	g	NN
cord-104282-90t1m430	220	23	for	for	IN
cord-104282-90t1m430	220	24	80	80	CD
cord-104282-90t1m430	220	25	i	i	PRP
cord-104282-90t1m430	220	26	rain	rain	VBP
cord-104282-90t1m430	220	27	.	.	.
cord-104282-90t1m430	221	1	The	the	DT
cord-104282-90t1m430	221	2	membrane	membrane	NN
cord-104282-90t1m430	221	3	fraction	fraction	NN
cord-104282-90t1m430	221	4	containing/3GaI	containing/3GaI	NNP
cord-104282-90t1m430	221	5	-	-	HYPH
cord-104282-90t1m430	221	6	ALDH	ALDH	NNP
cord-104282-90t1m430	221	7	was	be	VBD
cord-104282-90t1m430	221	8	treated	treat	VBN
cord-104282-90t1m430	221	9	•	•	NNP
cord-104282-90t1m430	221	10	with	with	IN
cord-104282-90t1m430	221	11	100	100	CD
cord-104282-90t1m430	221	12	mM	mm	CD
cord-104282-90t1m430	221	13	Na2CO3	Na2CO3	NNP
cord-104282-90t1m430	221	14	at	at	IN
cord-104282-90t1m430	221	15	0*C	0*c	CD
cord-104282-90t1m430	221	16	for	for	IN
cord-104282-90t1m430	221	17	30	30	CD
cord-104282-90t1m430	221	18	min	min	NN
cord-104282-90t1m430	221	19	,	,	,
cord-104282-90t1m430	221	20	and	and	CC
cord-104282-90t1m430	221	21	was	be	VBD
cord-104282-90t1m430	221	22	centrifuged	centrifuge	VBN
cord-104282-90t1m430	221	23	at	at	IN
cord-104282-90t1m430	221	24	88,000	88,000	CD
cord-104282-90t1m430	221	25	g	g	NN
cord-104282-90t1m430	221	26	for	for	IN
cord-104282-90t1m430	221	27	80	80	CD
cord-104282-90t1m430	221	28	min	min	NN
cord-104282-90t1m430	221	29	to	to	TO
cord-104282-90t1m430	221	30	separate	separate	VB
cord-104282-90t1m430	221	31	the	the	DT
cord-104282-90t1m430	221	32	pellet	pellet	NN
cord-104282-90t1m430	221	33	(	(	-LRB-
cord-104282-90t1m430	221	34	P	p	NN
cord-104282-90t1m430	221	35	)	)	-RRB-
cord-104282-90t1m430	221	36	from	from	IN
cord-104282-90t1m430	221	37	the	the	DT
cord-104282-90t1m430	221	38	supernatant	supernatant	NN
cord-104282-90t1m430	221	39	(	(	-LRB-
cord-104282-90t1m430	221	40	S	S	NNP
cord-104282-90t1m430	221	41	)	)	-RRB-
cord-104282-90t1m430	221	42	.	.	.
cord-104282-90t1m430	222	1	Each	each	DT
cord-104282-90t1m430	222	2	fraction	fraction	NN
cord-104282-90t1m430	222	3	was	be	VBD
cord-104282-90t1m430	222	4	assayed	assay	VBN
cord-104282-90t1m430	222	5	by	by	IN
cord-104282-90t1m430	222	6	immunoblotting	immunoblotte	VBG
cord-104282-90t1m430	222	7	using	use	VBG
cord-104282-90t1m430	222	8	mAb	mAb	NNS
cord-104282-90t1m430	222	9	to	to	IN
cord-104282-90t1m430	222	10	/3-galactosidase	/3-galactosidase	NNP
cord-104282-90t1m430	222	11	.	.	.
cord-104282-90t1m430	223	1	was	be	VBD
cord-104282-90t1m430	223	2	supposed	suppose	VBN
cord-104282-90t1m430	223	3	to	to	TO
cord-104282-90t1m430	223	4	remain	remain	VB
cord-104282-90t1m430	223	5	in	in	IN
cord-104282-90t1m430	223	6	the	the	DT
cord-104282-90t1m430	223	7	cytoplasm	cytoplasm	NN
cord-104282-90t1m430	223	8	when	when	WRB
cord-104282-90t1m430	223	9	expressedin	expressedin	NNP
cord-104282-90t1m430	223	10	COS-1	COS-1	NNP
cord-104282-90t1m430	223	11	cells	cell	NNS
cord-104282-90t1m430	223	12	under	under	IN
cord-104282-90t1m430	223	13	the	the	DT
cord-104282-90t1m430	223	14	control	control	NN
cord-104282-90t1m430	223	15	of	of	IN
cord-104282-90t1m430	223	16	the	the	DT
cord-104282-90t1m430	223	17	SV-40	SV-40	NNP
cord-104282-90t1m430	223	18	promoter	promoter	NN
cord-104282-90t1m430	223	19	.	.	.
cord-104282-90t1m430	224	1	The	the	DT
cord-104282-90t1m430	224	2	constructed	construct	VBN
cord-104282-90t1m430	224	3	expression	expression	NN
cord-104282-90t1m430	224	4	plasmid	plasmid	NN
cord-104282-90t1m430	224	5	(	(	-LRB-
cord-104282-90t1m430	224	6	pCHll0	pCHll0	NNP
cord-104282-90t1m430	224	7	/	/	SYM
cord-104282-90t1m430	224	8	ALDI	ALDI	NNP
cord-104282-90t1m430	224	9	-	-	HYPH
cord-104282-90t1m430	224	10	I	I	NNP
cord-104282-90t1m430	224	11	)	)	-RRB-
cord-104282-90t1m430	224	12	contains	contain	VBZ
cord-104282-90t1m430	224	13	the	the	DT
cord-104282-90t1m430	224	14	last	last	JJ
cord-104282-90t1m430	224	15	35	35	CD
cord-104282-90t1m430	224	16	amino	amino	NN
cord-104282-90t1m430	224	17	acids	acid	NNS
cord-104282-90t1m430	224	18	of	of	IN
cord-104282-90t1m430	224	19	msALDH	msALDH	NNP
cord-104282-90t1m430	224	20	cloned	clone	VBN
cord-104282-90t1m430	224	21	in	in	IN
cord-104282-90t1m430	224	22	-	-	HYPH
cord-104282-90t1m430	224	23	frame	frame	NN
cord-104282-90t1m430	224	24	to	to	IN
cord-104282-90t1m430	224	25	the	the	DT
cord-104282-90t1m430	224	26	3	3	CD
cord-104282-90t1m430	224	27	'	'	NN
cord-104282-90t1m430	224	28	end	end	NN
cord-104282-90t1m430	224	29	of/3-galactosidase	of/3-galactosidase	NNP
cord-104282-90t1m430	224	30	.	.	.
cord-104282-90t1m430	225	1	COS-1	COS-1	NNP
cord-104282-90t1m430	225	2	cells	cell	NNS
cord-104282-90t1m430	225	3	were	be	VBD
cord-104282-90t1m430	225	4	transfected	transfecte	VBN
cord-104282-90t1m430	225	5	with	with	IN
cord-104282-90t1m430	225	6	these	these	DT
cord-104282-90t1m430	225	7	DNAs	dna	NNS
cord-104282-90t1m430	225	8	and	and	CC
cord-104282-90t1m430	225	9	subjected	subject	VBN
cord-104282-90t1m430	225	10	to	to	IN
cord-104282-90t1m430	225	11	a	a	DT
cord-104282-90t1m430	225	12	crude	crude	JJ
cord-104282-90t1m430	225	13	subcellular	subcellular	JJ
cord-104282-90t1m430	225	14	fractionation	fractionation	NN
cord-104282-90t1m430	225	15	.	.	.
cord-104282-90t1m430	226	1	In	in	IN
cord-104282-90t1m430	226	2	this	this	DT
cord-104282-90t1m430	226	3	case	case	NN
cord-104282-90t1m430	226	4	,	,	,
cord-104282-90t1m430	226	5	the	the	DT
cord-104282-90t1m430	226	6	postnuclear	postnuclear	NN
cord-104282-90t1m430	226	7	supernatant	supernatant	NNP
cord-104282-90t1m430	226	8	was	be	VBD
cord-104282-90t1m430	226	9	centrifuged	centrifuge	VBN
cord-104282-90t1m430	226	10	at	at	IN
cord-104282-90t1m430	226	11	88,000	88,000	CD
cord-104282-90t1m430	226	12	g	g	NN
cord-104282-90t1m430	226	13	for	for	IN
cord-104282-90t1m430	226	14	80	80	CD
cord-104282-90t1m430	226	15	min	min	NN
cord-104282-90t1m430	226	16	to	to	TO
cord-104282-90t1m430	226	17	separate	separate	VB
cord-104282-90t1m430	226	18	the	the	DT
cord-104282-90t1m430	226	19	membrane	membrane	NN
cord-104282-90t1m430	226	20	fraction	fraction	NN
cord-104282-90t1m430	226	21	(	(	-LRB-
cord-104282-90t1m430	226	22	P	p	NN
cord-104282-90t1m430	226	23	,	,	,
cord-104282-90t1m430	226	24	containing	contain	VBG
cord-104282-90t1m430	226	25	mostly	mostly	RB
cord-104282-90t1m430	226	26	mitochondria	mitochondrion	NNS
cord-104282-90t1m430	226	27	and	and	CC
cord-104282-90t1m430	226	28	microsomes	microsome	NNS
cord-104282-90t1m430	226	29	)	)	-RRB-
cord-104282-90t1m430	226	30	from	from	IN
cord-104282-90t1m430	226	31	the	the	DT
cord-104282-90t1m430	226	32	cytosol	cytosol	NN
cord-104282-90t1m430	226	33	fraction	fraction	NN
cord-104282-90t1m430	226	34	(	(	-LRB-
cord-104282-90t1m430	226	35	S	S	NNP
cord-104282-90t1m430	226	36	)	)	-RRB-
cord-104282-90t1m430	226	37	.	.	.
cord-104282-90t1m430	227	1	Immunoblotting	immunoblotte	VBG
cord-104282-90t1m430	227	2	using	use	VBG
cord-104282-90t1m430	227	3	mAb	mAb	NNS
cord-104282-90t1m430	227	4	to	to	IN
cord-104282-90t1m430	227	5	E.	E.	NNP
cord-104282-90t1m430	227	6	coli	coli	VBZ
cord-104282-90t1m430	227	7	~-galactosidase	~-galactosidase	NNS
cord-104282-90t1m430	227	8	revealed	reveal	VBD
cord-104282-90t1m430	227	9	that	that	IN
cord-104282-90t1m430	227	10	wild	wild	JJ
cord-104282-90t1m430	227	11	-	-	HYPH
cord-104282-90t1m430	227	12	type	type	NN
cord-104282-90t1m430	227	13	~-galactosidase	~-galactosidase	NFP
cord-104282-90t1m430	227	14	was	be	VBD
cord-104282-90t1m430	227	15	recovered	recover	VBN
cord-104282-90t1m430	227	16	,	,	,
cord-104282-90t1m430	227	17	as	as	IN
cord-104282-90t1m430	227	18	expected	expect	VBN
cord-104282-90t1m430	227	19	,	,	,
cord-104282-90t1m430	227	20	in	in	IN
cord-104282-90t1m430	227	21	the	the	DT
cord-104282-90t1m430	227	22	S	S	NNP
cord-104282-90t1m430	227	23	fraction	fraction	NN
cord-104282-90t1m430	227	24	,	,	,
cord-104282-90t1m430	227	25	whereas	whereas	IN
cord-104282-90t1m430	227	26	~-galactosidase	~-galactosidase	NNP
cord-104282-90t1m430	227	27	/	/	SYM
cord-104282-90t1m430	227	28	ALDH	ALDH	NNP
cord-104282-90t1m430	227	29	chimera	chimera	NN
cord-104282-90t1m430	227	30	was	be	VBD
cord-104282-90t1m430	227	31	found	find	VBN
cord-104282-90t1m430	227	32	concentrated	concentrated	JJ
cord-104282-90t1m430	227	33	in	in	IN
cord-104282-90t1m430	227	34	the	the	DT
cord-104282-90t1m430	227	35	P	p	NN
cord-104282-90t1m430	227	36	fraction	fraction	NN
cord-104282-90t1m430	228	1	(	(	-LRB-
cord-104282-90t1m430	228	2	Fig	Fig	NNP
cord-104282-90t1m430	228	3	.	.	.
cord-104282-90t1m430	228	4	5	5	CD
cord-104282-90t1m430	228	5	B	b	NN
cord-104282-90t1m430	228	6	)	)	-RRB-
cord-104282-90t1m430	228	7	.	.	.
cord-104282-90t1m430	229	1	In	in	IN
cord-104282-90t1m430	229	2	addition	addition	NN
cord-104282-90t1m430	229	3	,	,	,
cord-104282-90t1m430	229	4	the	the	DT
cord-104282-90t1m430	229	5	chimera	chimera	NN
cord-104282-90t1m430	229	6	in	in	IN
cord-104282-90t1m430	229	7	the	the	DT
cord-104282-90t1m430	229	8	P	p	NN
cord-104282-90t1m430	229	9	fraction	fraction	NN
cord-104282-90t1m430	229	10	was	be	VBD
cord-104282-90t1m430	229	11	resistant	resistant	JJ
cord-104282-90t1m430	229	12	to	to	IN
cord-104282-90t1m430	229	13	alkali	alkali	JJ
cord-104282-90t1m430	229	14	extraction	extraction	NN
cord-104282-90t1m430	229	15	,	,	,
cord-104282-90t1m430	229	16	indicaring	indicare	VBG
cord-104282-90t1m430	229	17	a	a	DT
cord-104282-90t1m430	229	18	fight	fight	NN
cord-104282-90t1m430	229	19	anchoring	anchoring	NN
cord-104282-90t1m430	229	20	of	of	IN
cord-104282-90t1m430	229	21	this	this	DT
cord-104282-90t1m430	229	22	protein	protein	NN
cord-104282-90t1m430	229	23	to	to	IN
cord-104282-90t1m430	229	24	intracellular	intracellular	JJ
cord-104282-90t1m430	229	25	membranes	membrane	NNS
cord-104282-90t1m430	229	26	(	(	-LRB-
cord-104282-90t1m430	229	27	Fig	fig	NN
cord-104282-90t1m430	229	28	.	.	.
cord-104282-90t1m430	229	29	5	5	CD
cord-104282-90t1m430	229	30	B	b	NN
cord-104282-90t1m430	229	31	)	)	-RRB-
cord-104282-90t1m430	229	32	.	.	.
cord-104282-90t1m430	230	1	Indirect	indirect	JJ
cord-104282-90t1m430	230	2	immunofluorescence	immunofluorescence	NN
cord-104282-90t1m430	230	3	microscopy	microscopy	NNS
cord-104282-90t1m430	230	4	showed	show	VBD
cord-104282-90t1m430	230	5	that	that	IN
cord-104282-90t1m430	230	6	~-gaiactosidase	~-gaiactosidase	NNP
cord-104282-90t1m430	230	7	was	be	VBD
cord-104282-90t1m430	230	8	distributed	distribute	VBN
cord-104282-90t1m430	230	9	diffusely	diffusely	RB
cord-104282-90t1m430	230	10	throughout	throughout	IN
cord-104282-90t1m430	230	11	the	the	DT
cord-104282-90t1m430	230	12	cytoplasm	cytoplasm	NN
cord-104282-90t1m430	231	1	(	(	-LRB-
cord-104282-90t1m430	231	2	Fig	Fig	NNP
cord-104282-90t1m430	231	3	.	.	.
cord-104282-90t1m430	231	4	6	6	CD
cord-104282-90t1m430	231	5	A	a	NN
cord-104282-90t1m430	231	6	)	)	-RRB-
cord-104282-90t1m430	231	7	.	.	.
cord-104282-90t1m430	232	1	However	however	RB
cord-104282-90t1m430	232	2	,	,	,
cord-104282-90t1m430	232	3	attachment	attachment	NN
cord-104282-90t1m430	232	4	of	of	IN
cord-104282-90t1m430	232	5	the	the	DT
cord-104282-90t1m430	232	6	carboxylterminal	carboxylterminal	JJ
cord-104282-90t1m430	232	7	35	35	CD
cord-104282-90t1m430	232	8	amino	amino	NN
cord-104282-90t1m430	232	9	acids	acid	NNS
cord-104282-90t1m430	232	10	of	of	IN
cord-104282-90t1m430	232	11	msALDH	msALDH	NNP
cord-104282-90t1m430	232	12	to	to	IN
cord-104282-90t1m430	232	13	~-galactosidase	~-galactosidase	NNP
cord-104282-90t1m430	232	14	resulted	result	VBD
cord-104282-90t1m430	232	15	in	in	IN
cord-104282-90t1m430	232	16	the	the	DT
cord-104282-90t1m430	232	17	reticular	reticular	JJ
cord-104282-90t1m430	232	18	pattern	pattern	NN
cord-104282-90t1m430	232	19	of	of	IN
cord-104282-90t1m430	232	20	staining	staining	NN
cord-104282-90t1m430	232	21	that	that	WDT
cord-104282-90t1m430	232	22	surrounded	surround	VBD
cord-104282-90t1m430	232	23	the	the	DT
cord-104282-90t1m430	232	24	nucleus	nucleus	NN
cord-104282-90t1m430	232	25	and	and	CC
cord-104282-90t1m430	232	26	extended	extend	VBN
cord-104282-90t1m430	232	27	throughout	throughout	IN
cord-104282-90t1m430	232	28	the	the	DT
cord-104282-90t1m430	232	29	cytoplasm	cytoplasm	NN
cord-104282-90t1m430	233	1	(	(	-LRB-
cord-104282-90t1m430	233	2	Fig	Fig	NNP
cord-104282-90t1m430	233	3	.	.	.
cord-104282-90t1m430	233	4	6	6	CD
cord-104282-90t1m430	233	5	 	 	_SP
cord-104282-90t1m430	233	6	B	B	NNP
cord-104282-90t1m430	233	7	)	)	-RRB-
cord-104282-90t1m430	233	8	.	.	.
cord-104282-90t1m430	234	1	Double	double	JJ
cord-104282-90t1m430	234	2	indirect	indirect	JJ
cord-104282-90t1m430	234	3	immunofluorescence	immunofluorescence	NN
cord-104282-90t1m430	234	4	microscopy	microscopy	NNS
cord-104282-90t1m430	234	5	using	use	VBG
cord-104282-90t1m430	234	6	polyclonal	polyclonal	JJ
cord-104282-90t1m430	234	7	anti	anti	JJ
cord-104282-90t1m430	234	8	-	-	JJ
cord-104282-90t1m430	234	9	PDI	pdi	JJ
cord-104282-90t1m430	234	10	antibody	antibody	NN
cord-104282-90t1m430	234	11	showed	show	VBD
cord-104282-90t1m430	234	12	the	the	DT
cord-104282-90t1m430	234	13	colocalization	colocalization	NN
cord-104282-90t1m430	234	14	of	of	IN
cord-104282-90t1m430	234	15	/3-galactosidase	/3-galactosidase	NNP
cord-104282-90t1m430	234	16	/	/	SYM
cord-104282-90t1m430	234	17	ALDH	ALDH	NNP
cord-104282-90t1m430	234	18	chimera	chimera	NN
cord-104282-90t1m430	234	19	and	and	CC
cord-104282-90t1m430	234	20	PDI	PDI	NNP
cord-104282-90t1m430	234	21	in	in	IN
cord-104282-90t1m430	234	22	the	the	DT
cord-104282-90t1m430	234	23	ER	er	NN
cord-104282-90t1m430	234	24	(	(	-LRB-
cord-104282-90t1m430	234	25	Fig	fig	NN
cord-104282-90t1m430	234	26	.	.	.
cord-104282-90t1m430	234	27	6	6	CD
cord-104282-90t1m430	234	28	 	 	_SP
cord-104282-90t1m430	234	29	C	C	NNP
cord-104282-90t1m430	234	30	)	)	-RRB-
cord-104282-90t1m430	234	31	.	.	.
cord-104282-90t1m430	235	1	These	these	DT
cord-104282-90t1m430	235	2	results	result	NNS
cord-104282-90t1m430	235	3	suggested	suggest	VBD
cord-104282-90t1m430	235	4	that	that	IN
cord-104282-90t1m430	235	5	the	the	DT
cord-104282-90t1m430	235	6	carboxyl	carboxyl	NN
cord-104282-90t1m430	235	7	-	-	HYPH
cord-104282-90t1m430	235	8	terminai	terminai	NNP
cord-104282-90t1m430	235	9	35	35	CD
cord-104282-90t1m430	235	10	amino	amino	NN
cord-104282-90t1m430	235	11	acids	acid	NNS
cord-104282-90t1m430	235	12	were	be	VBD
cord-104282-90t1m430	235	13	sufficient	sufficient	JJ
cord-104282-90t1m430	235	14	for	for	IN
cord-104282-90t1m430	235	15	targeting	target	VBG
cord-104282-90t1m430	235	16	of	of	IN
cord-104282-90t1m430	235	17	E.	E.	NNP
cord-104282-90t1m430	235	18	coli	coli	NNS
cord-104282-90t1m430	236	1	#	#	$
cord-104282-90t1m430	237	1	-galactosidase	-galactosidase	:
cord-104282-90t1m430	237	2	to	to	IN
cord-104282-90t1m430	237	3	the	the	DT
cord-104282-90t1m430	237	4	ER	ER	NNP
cord-104282-90t1m430	237	5	membrane	membrane	NN
cord-104282-90t1m430	237	6	.	.	.
cord-104282-90t1m430	238	1	We	-PRON-	PRP
cord-104282-90t1m430	238	2	attempted	attempt	VBD
cord-104282-90t1m430	238	3	to	to	TO
cord-104282-90t1m430	238	4	define	define	VB
cord-104282-90t1m430	238	5	the	the	DT
cord-104282-90t1m430	238	6	sequence	sequence	NN
cord-104282-90t1m430	238	7	requirement	requirement	NN
cord-104282-90t1m430	238	8	for	for	IN
cord-104282-90t1m430	238	9	ER	ER	NNP
cord-104282-90t1m430	238	10	targeting	target	VBG
cord-104282-90t1m430	238	11	within	within	IN
cord-104282-90t1m430	238	12	the	the	DT
cord-104282-90t1m430	238	13	last	last	JJ
cord-104282-90t1m430	238	14	35	35	CD
cord-104282-90t1m430	238	15	amino	amino	NN
cord-104282-90t1m430	238	16	acids	acid	NNS
cord-104282-90t1m430	238	17	of	of	IN
cord-104282-90t1m430	238	18	msALDH	msALDH	NNP
cord-104282-90t1m430	238	19	,	,	,
cord-104282-90t1m430	238	20	which	which	WDT
cord-104282-90t1m430	238	21	is	be	VBZ
cord-104282-90t1m430	238	22	composed	compose	VBN
cord-104282-90t1m430	238	23	of	of	IN
cord-104282-90t1m430	238	24	three	three	CD
cord-104282-90t1m430	238	25	regions	region	NNS
cord-104282-90t1m430	238	26	as	as	IN
cord-104282-90t1m430	238	27	shown	show	VBN
cord-104282-90t1m430	238	28	in	in	IN
cord-104282-90t1m430	238	29	Fig	Fig	NNP
cord-104282-90t1m430	238	30	.	.	.
cord-104282-90t1m430	239	1	l	l	NNP
cord-104282-90t1m430	239	2	,	,	,
cord-104282-90t1m430	239	3	and	and	CC
cord-104282-90t1m430	239	4	we	-PRON-	PRP
cord-104282-90t1m430	239	5	constructed	construct	VBD
cord-104282-90t1m430	239	6	a	a	DT
cord-104282-90t1m430	239	7	series	series	NN
cord-104282-90t1m430	239	8	of	of	IN
cord-104282-90t1m430	239	9	CAT	CAT	NNP
cord-104282-90t1m430	239	10	/	/	SYM
cord-104282-90t1m430	239	11	ALDH	ALDH	NNP
cord-104282-90t1m430	239	12	chimeras	chimera	NNS
cord-104282-90t1m430	239	13	(	(	-LRB-
cord-104282-90t1m430	239	14	CAT	CAT	NNP
cord-104282-90t1m430	239	15	/	/	SYM
cord-104282-90t1m430	239	16	ALDH1	ALDH1	NNP
cord-104282-90t1m430	239	17	-	-	HYPH
cord-104282-90t1m430	239	18	4	4	CD
cord-104282-90t1m430	239	19	)	)	-RRB-
cord-104282-90t1m430	240	1	(	(	-LRB-
cord-104282-90t1m430	240	2	Fig	Fig	NNP
cord-104282-90t1m430	240	3	.	.	NNP
cord-104282-90t1m430	240	4	7	7	CD
cord-104282-90t1m430	240	5	)	)	-RRB-
cord-104282-90t1m430	240	6	.	.	.
cord-104282-90t1m430	241	1	CAT	CAT	NNP
cord-104282-90t1m430	241	2	/	/	SYM
cord-104282-90t1m430	241	3	ALDH1	ALDH1	NNP
cord-104282-90t1m430	241	4	contains	contain	VBZ
cord-104282-90t1m430	241	5	the	the	DT
cord-104282-90t1m430	241	6	last	last	JJ
cord-104282-90t1m430	241	7	35	35	CD
cord-104282-90t1m430	241	8	amino	amino	NN
cord-104282-90t1m430	241	9	acids	acid	NNS
cord-104282-90t1m430	241	10	of	of	IN
cord-104282-90t1m430	241	11	msALDH	msALDH	NNS
cord-104282-90t1m430	241	12	at	at	IN
cord-104282-90t1m430	241	13	the	the	DT
cord-104282-90t1m430	241	14	carboxyl	carboxyl	NN
cord-104282-90t1m430	241	15	terminus	terminus	NN
cord-104282-90t1m430	241	16	of	of	IN
cord-104282-90t1m430	241	17	CAT	cat	NN
cord-104282-90t1m430	241	18	,	,	,
cord-104282-90t1m430	241	19	while	while	IN
cord-104282-90t1m430	241	20	CAT/	CAT/	NNP
cord-104282-90t1m430	241	21	ALDH2	ALDH2	NNP
cord-104282-90t1m430	241	22	-	-	HYPH
cord-104282-90t1m430	241	23	4	4	CD
cord-104282-90t1m430	241	24	chimeras	chimera	NNS
cord-104282-90t1m430	241	25	lack	lack	VBP
cord-104282-90t1m430	241	26	either	either	CC
cord-104282-90t1m430	241	27	the	the	DT
cord-104282-90t1m430	241	28	tail	tail	NN
cord-104282-90t1m430	241	29	,	,	,
cord-104282-90t1m430	241	30	stem	stem	NN
cord-104282-90t1m430	241	31	,	,	,
cord-104282-90t1m430	241	32	or	or	CC
cord-104282-90t1m430	241	33	both	both	DT
cord-104282-90t1m430	241	34	regions	region	NNS
cord-104282-90t1m430	241	35	of	of	IN
cord-104282-90t1m430	241	36	msALDH	msALDH	NNP
cord-104282-90t1m430	241	37	.	.	.
cord-104282-90t1m430	242	1	COS-1	COS-1	NNP
cord-104282-90t1m430	242	2	cells	cell	NNS
cord-104282-90t1m430	242	3	were	be	VBD
cord-104282-90t1m430	242	4	transfected	transfecte	VBN
cord-104282-90t1m430	242	5	with	with	IN
cord-104282-90t1m430	242	6	these	these	DT
cord-104282-90t1m430	242	7	cDNAs	cdna	NNS
cord-104282-90t1m430	242	8	in	in	IN
cord-104282-90t1m430	242	9	the	the	DT
cord-104282-90t1m430	242	10	pMIW	pmiw	JJ
cord-104282-90t1m430	242	11	expression	expression	NN
cord-104282-90t1m430	242	12	vector	vector	NN
cord-104282-90t1m430	242	13	that	that	WDT
cord-104282-90t1m430	242	14	possesses	possess	VBZ
cord-104282-90t1m430	242	15	/3-actin	/3-actin	NNP
cord-104282-90t1m430	242	16	promoter	promoter	NN
cord-104282-90t1m430	242	17	and	and	CC
cord-104282-90t1m430	242	18	Rous	rous	JJ
cord-104282-90t1m430	242	19	sarcoma	sarcoma	NN
cord-104282-90t1m430	242	20	enhancer	enhancer	NN
cord-104282-90t1m430	242	21	.	.	.
cord-104282-90t1m430	243	1	The	the	DT
cord-104282-90t1m430	243	2	intracellular	intracellular	JJ
cord-104282-90t1m430	243	3	localization	localization	NN
cord-104282-90t1m430	243	4	of	of	IN
cord-104282-90t1m430	243	5	the	the	DT
cord-104282-90t1m430	243	6	expressed	express	VBN
cord-104282-90t1m430	243	7	proteins	protein	NNS
cord-104282-90t1m430	243	8	were	be	VBD
cord-104282-90t1m430	243	9	determined	determine	VBN
cord-104282-90t1m430	243	10	by	by	IN
cord-104282-90t1m430	243	11	indirect	indirect	JJ
cord-104282-90t1m430	243	12	immunofluorescence	immunofluorescence	NN
cord-104282-90t1m430	243	13	microscopy	microscopy	NNS
cord-104282-90t1m430	243	14	using	use	VBG
cord-104282-90t1m430	243	15	anti	anti	JJ
cord-104282-90t1m430	243	16	-	-	JJ
cord-104282-90t1m430	243	17	CAT	cat	JJ
cord-104282-90t1m430	243	18	antibody	antibody	NN
cord-104282-90t1m430	243	19	.	.	.
cord-104282-90t1m430	244	1	Wild	wild	JJ
cord-104282-90t1m430	244	2	-	-	HYPH
cord-104282-90t1m430	244	3	type	type	NN
cord-104282-90t1m430	244	4	CAT	cat	NN
cord-104282-90t1m430	244	5	was	be	VBD
cord-104282-90t1m430	244	6	distributed	distribute	VBN
cord-104282-90t1m430	244	7	,	,	,
cord-104282-90t1m430	244	8	as	as	IN
cord-104282-90t1m430	244	9	expected	expect	VBN
cord-104282-90t1m430	244	10	,	,	,
cord-104282-90t1m430	244	11	throughout	throughout	IN
cord-104282-90t1m430	244	12	the	the	DT
cord-104282-90t1m430	244	13	cytoplasm	cytoplasm	NN
cord-104282-90t1m430	244	14	.	.	.
cord-104282-90t1m430	245	1	In	in	IN
cord-104282-90t1m430	245	2	addition	addition	NN
cord-104282-90t1m430	245	3	,	,	,
cord-104282-90t1m430	245	4	it	-PRON-	PRP
cord-104282-90t1m430	245	5	was	be	VBD
cord-104282-90t1m430	245	6	also	also	RB
cord-104282-90t1m430	245	7	detected	detect	VBN
cord-104282-90t1m430	245	8	in	in	IN
cord-104282-90t1m430	245	9	the	the	DT
cord-104282-90t1m430	245	10	nucleoplasm	nucleoplasm	NN
cord-104282-90t1m430	245	11	(	(	-LRB-
cord-104282-90t1m430	245	12	Fig	Fig	NNP
cord-104282-90t1m430	245	13	.	.	.
cord-104282-90t1m430	245	14	8	8	CD
cord-104282-90t1m430	245	15	A	a	NN
cord-104282-90t1m430	245	16	)	)	-RRB-
cord-104282-90t1m430	245	17	.	.	.
cord-104282-90t1m430	246	1	CAT	CAT	NNP
cord-104282-90t1m430	246	2	/	/	SYM
cord-104282-90t1m430	246	3	ALDH1	ALDH1	NNP
cord-104282-90t1m430	246	4	showed	show	VBD
cord-104282-90t1m430	246	5	the	the	DT
cord-104282-90t1m430	246	6	characteristic	characteristic	JJ
cord-104282-90t1m430	246	7	ER	er	NN
cord-104282-90t1m430	246	8	staining	staining	NN
cord-104282-90t1m430	246	9	pattern	pattern	NN
cord-104282-90t1m430	246	10	(	(	-LRB-
cord-104282-90t1m430	246	11	Fig	fig	NN
cord-104282-90t1m430	246	12	.	.	.
cord-104282-90t1m430	246	13	8	8	CD
cord-104282-90t1m430	246	14	B	b	NN
cord-104282-90t1m430	246	15	)	)	-RRB-
cord-104282-90t1m430	246	16	,	,	,
cord-104282-90t1m430	246	17	which	which	WDT
cord-104282-90t1m430	246	18	was	be	VBD
cord-104282-90t1m430	246	19	confirmed	confirm	VBN
cord-104282-90t1m430	246	20	by	by	IN
cord-104282-90t1m430	246	21	double	double	JJ
cord-104282-90t1m430	246	22	indirect	indirect	JJ
cord-104282-90t1m430	246	23	immunofluorescence	immunofluorescence	NN
cord-104282-90t1m430	246	24	microscopy	microscopy	NN
cord-104282-90t1m430	246	25	using	use	VBG
cord-104282-90t1m430	246	26	PDI	PDI	NNP
cord-104282-90t1m430	246	27	mAb	mAb	NNPS
cord-104282-90t1m430	246	28	(	(	-LRB-
cord-104282-90t1m430	246	29	Fig	Fig	NNP
cord-104282-90t1m430	246	30	.	.	.
cord-104282-90t1m430	246	31	8	8	CD
cord-104282-90t1m430	246	32	C	c	NN
cord-104282-90t1m430	246	33	)	)	-RRB-
cord-104282-90t1m430	246	34	.	.	.
cord-104282-90t1m430	247	1	This	this	DT
cord-104282-90t1m430	247	2	result	result	NN
cord-104282-90t1m430	247	3	was	be	VBD
cord-104282-90t1m430	247	4	similar	similar	JJ
cord-104282-90t1m430	247	5	to	to	IN
cord-104282-90t1m430	247	6	that	that	DT
cord-104282-90t1m430	247	7	obtained	obtain	VBN
cord-104282-90t1m430	247	8	with/5-gaiactosidase	with/5-gaiactosidase	CD
cord-104282-90t1m430	247	9	/	/	SYM
cord-104282-90t1m430	247	10	ALDH	ALDH	NNP
cord-104282-90t1m430	247	11	(	(	-LRB-
cord-104282-90t1m430	247	12	Fig	Fig	NNP
cord-104282-90t1m430	247	13	.	.	NNP
cord-104282-90t1m430	247	14	6	6	CD
cord-104282-90t1m430	247	15	,	,	,
cord-104282-90t1m430	247	16	B	b	NN
cord-104282-90t1m430	247	17	and	and	CC
cord-104282-90t1m430	247	18	C	C	NNP
cord-104282-90t1m430	247	19	)	)	-RRB-
cord-104282-90t1m430	247	20	.	.	.
cord-104282-90t1m430	248	1	Both	both	DT
cord-104282-90t1m430	248	2	CAT	CAT	NNP
cord-104282-90t1m430	248	3	/	/	SYM
cord-104282-90t1m430	248	4	ALDI	ALDI	NNP
cord-104282-90t1m430	248	5	-	-	HYPH
cord-104282-90t1m430	248	6	I2	I2	NNP
cord-104282-90t1m430	248	7	and	and	CC
cord-104282-90t1m430	248	8	CAT	CAT	NNP
cord-104282-90t1m430	248	9	/	/	SYM
cord-104282-90t1m430	248	10	ALDH3	ALDH3	NNP
cord-104282-90t1m430	248	11	were	be	VBD
cord-104282-90t1m430	248	12	also	also	RB
cord-104282-90t1m430	248	13	found	find	VBN
cord-104282-90t1m430	248	14	to	to	IN
cord-104282-90t1m430	248	15	colocalize	colocalize	VB
cord-104282-90t1m430	248	16	with	with	IN
cord-104282-90t1m430	248	17	endogenous	endogenous	JJ
cord-104282-90t1m430	248	18	PDI	PDI	NNP
cord-104282-90t1m430	248	19	in	in	IN
cord-104282-90t1m430	248	20	the	the	DT
cord-104282-90t1m430	248	21	ER	er	NN
cord-104282-90t1m430	248	22	(	(	-LRB-
cord-104282-90t1m430	248	23	Fig	fig	NN
cord-104282-90t1m430	248	24	.	.	.
cord-104282-90t1m430	249	1	8	8	CD
cord-104282-90t1m430	249	2	,	,	,
cord-104282-90t1m430	249	3	D	D	NNP
cord-104282-90t1m430	249	4	-	-	HYPH
cord-104282-90t1m430	249	5	G	G	NNP
cord-104282-90t1m430	249	6	)	)	-RRB-
cord-104282-90t1m430	249	7	.	.	.
cord-104282-90t1m430	250	1	On	on	IN
cord-104282-90t1m430	250	2	the	the	DT
cord-104282-90t1m430	250	3	other	other	JJ
cord-104282-90t1m430	250	4	hand	hand	NN
cord-104282-90t1m430	250	5	,	,	,
cord-104282-90t1m430	250	6	CAT	CAT	NNP
cord-104282-90t1m430	250	7	/	/	SYM
cord-104282-90t1m430	250	8	ALDH4	ALDH4	NNP
cord-104282-90t1m430	250	9	containing	contain	VBG
cord-104282-90t1m430	250	10	only	only	RB
cord-104282-90t1m430	250	11	the	the	DT
cord-104282-90t1m430	250	12	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	250	13	domain	domain	NN
cord-104282-90t1m430	250	14	of	of	IN
cord-104282-90t1m430	250	15	msALDH	msALDH	NNP
cord-104282-90t1m430	250	16	at	at	IN
cord-104282-90t1m430	250	17	its	-PRON-	PRP$
cord-104282-90t1m430	250	18	carboxyl	carboxyl	NN
cord-104282-90t1m430	250	19	terminus	terminus	NN
cord-104282-90t1m430	250	20	showed	show	VBD
cord-104282-90t1m430	250	21	a	a	DT
cord-104282-90t1m430	250	22	similar	similar	JJ
cord-104282-90t1m430	250	23	pattern	pattern	NN
cord-104282-90t1m430	250	24	of	of	IN
cord-104282-90t1m430	250	25	staining	stain	VBG
cord-104282-90t1m430	250	26	to	to	IN
cord-104282-90t1m430	250	27	that	that	DT
cord-104282-90t1m430	250	28	of	of	IN
cord-104282-90t1m430	250	29	wild	wild	JJ
cord-104282-90t1m430	250	30	-	-	HYPH
cord-104282-90t1m430	250	31	type	type	NN
cord-104282-90t1m430	250	32	CAT	cat	NN
cord-104282-90t1m430	250	33	.	.	.
cord-104282-90t1m430	251	1	This	this	DT
cord-104282-90t1m430	251	2	chimera	chimera	NN
cord-104282-90t1m430	251	3	remained	remain	VBD
cord-104282-90t1m430	251	4	not	not	RB
cord-104282-90t1m430	251	5	only	only	RB
cord-104282-90t1m430	251	6	in	in	IN
cord-104282-90t1m430	251	7	the	the	DT
cord-104282-90t1m430	251	8	cytoplasm	cytoplasm	NN
cord-104282-90t1m430	251	9	,	,	,
cord-104282-90t1m430	251	10	but	but	CC
cord-104282-90t1m430	251	11	in	in	IN
cord-104282-90t1m430	251	12	the	the	DT
cord-104282-90t1m430	251	13	nucleoplasm	nucleoplasm	NN
cord-104282-90t1m430	251	14	as	as	RB
cord-104282-90t1m430	251	15	well	well	RB
cord-104282-90t1m430	251	16	(	(	-LRB-
cord-104282-90t1m430	251	17	Fig	Fig	NNP
cord-104282-90t1m430	251	18	.	.	.
cord-104282-90t1m430	251	19	8	8	CD
cord-104282-90t1m430	251	20	H	h	NN
cord-104282-90t1m430	251	21	)	)	-RRB-
cord-104282-90t1m430	251	22	,	,	,
cord-104282-90t1m430	251	23	and	and	CC
cord-104282-90t1m430	251	24	it	-PRON-	PRP
cord-104282-90t1m430	251	25	did	do	VBD
cord-104282-90t1m430	251	26	not	not	RB
cord-104282-90t1m430	251	27	colocaiize	colocaiize	VB
cord-104282-90t1m430	251	28	with	with	IN
cord-104282-90t1m430	251	29	PDI	PDI	NNP
cord-104282-90t1m430	251	30	(	(	-LRB-
cord-104282-90t1m430	251	31	Fig	Fig	NNP
cord-104282-90t1m430	251	32	.	.	.
cord-104282-90t1m430	251	33	8	8	CD
cord-104282-90t1m430	251	34	I	i	NN
cord-104282-90t1m430	251	35	)	)	-RRB-
cord-104282-90t1m430	251	36	.	.	.
cord-104282-90t1m430	252	1	The	the	DT
cord-104282-90t1m430	252	2	simplest	simple	JJS
cord-104282-90t1m430	252	3	interpretation	interpretation	NN
cord-104282-90t1m430	252	4	of	of	IN
cord-104282-90t1m430	252	5	these	these	DT
cord-104282-90t1m430	252	6	results	result	NNS
cord-104282-90t1m430	252	7	is	be	VBZ
cord-104282-90t1m430	252	8	that	that	IN
cord-104282-90t1m430	252	9	both	both	CC
cord-104282-90t1m430	252	10	the	the	DT
cord-104282-90t1m430	252	11	stem	stem	NN
cord-104282-90t1m430	252	12	and	and	CC
cord-104282-90t1m430	252	13	tail	tail	NN
cord-104282-90t1m430	252	14	regions	region	NNS
cord-104282-90t1m430	252	15	appear	appear	VBP
cord-104282-90t1m430	252	16	to	to	TO
cord-104282-90t1m430	252	17	contain	contain	VB
cord-104282-90t1m430	252	18	ERtargeting	ertargete	VBG
cord-104282-90t1m430	252	19	information	information	NN
cord-104282-90t1m430	252	20	of	of	IN
cord-104282-90t1m430	252	21	msALDH	msALDH	NNP
cord-104282-90t1m430	252	22	.	.	.
cord-104282-90t1m430	253	1	To	to	TO
cord-104282-90t1m430	253	2	elucidate	elucidate	VB
cord-104282-90t1m430	253	3	more	more	RBR
cord-104282-90t1m430	253	4	narrowly	narrowly	RB
cord-104282-90t1m430	253	5	the	the	DT
cord-104282-90t1m430	253	6	sequence	sequence	NN
cord-104282-90t1m430	253	7	requirement	requirement	NN
cord-104282-90t1m430	253	8	within	within	IN
cord-104282-90t1m430	253	9	the	the	DT
cord-104282-90t1m430	253	10	stem	stem	NN
cord-104282-90t1m430	253	11	region	region	NN
cord-104282-90t1m430	253	12	for	for	IN
cord-104282-90t1m430	253	13	ER	ER	NNP
cord-104282-90t1m430	253	14	targeting	targeting	NN
cord-104282-90t1m430	253	15	,	,	,
cord-104282-90t1m430	253	16	we	-PRON-	PRP
cord-104282-90t1m430	253	17	constructed	construct	VBD
cord-104282-90t1m430	253	18	an	an	DT
cord-104282-90t1m430	253	19	additional	additional	JJ
cord-104282-90t1m430	253	20	series	series	NN
cord-104282-90t1m430	253	21	of	of	IN
cord-104282-90t1m430	253	22	CAT	CAT	NNP
cord-104282-90t1m430	253	23	/	/	SYM
cord-104282-90t1m430	253	24	ALDH	ALDH	NNP
cord-104282-90t1m430	253	25	chimeras	chimera	NNS
cord-104282-90t1m430	253	26	(	(	-LRB-
cord-104282-90t1m430	253	27	CAT	cat	NN
cord-104282-90t1m430	253	28	/	/	SYM
cord-104282-90t1m430	253	29	ALDH5	ALDH5	NNP
cord-104282-90t1m430	253	30	-	-	HYPH
cord-104282-90t1m430	253	31	10	10	CD
cord-104282-90t1m430	253	32	)	)	-RRB-
cord-104282-90t1m430	253	33	(	(	-LRB-
cord-104282-90t1m430	253	34	Fig	Fig	NNP
cord-104282-90t1m430	253	35	.	.	.
cord-104282-90t1m430	253	36	9	9	CD
cord-104282-90t1m430	253	37	)	)	-RRB-
cord-104282-90t1m430	253	38	,	,	,
cord-104282-90t1m430	253	39	in	in	IN
cord-104282-90t1m430	253	40	which	which	WDT
cord-104282-90t1m430	253	41	three	three	CD
cord-104282-90t1m430	253	42	to	to	TO
cord-104282-90t1m430	253	43	seven	seven	CD
cord-104282-90t1m430	253	44	amino	amino	NN
cord-104282-90t1m430	253	45	acids	acid	NNS
cord-104282-90t1m430	253	46	of	of	IN
cord-104282-90t1m430	253	47	the	the	DT
cord-104282-90t1m430	253	48	stem	stem	NN
cord-104282-90t1m430	253	49	region	region	NN
cord-104282-90t1m430	253	50	of	of	IN
cord-104282-90t1m430	253	51	either	either	DT
cord-104282-90t1m430	253	52	CAT	CAT	NNP
cord-104282-90t1m430	253	53	/	/	SYM
cord-104282-90t1m430	253	54	ALDH1	ALDH1	NNP
cord-104282-90t1m430	253	55	or	or	CC
cord-104282-90t1m430	253	56	CAT	CAT	NNP
cord-104282-90t1m430	253	57	/	/	SYM
cord-104282-90t1m430	253	58	ALDI	ALDI	NNP
cord-104282-90t1m430	253	59	-	-	HYPH
cord-104282-90t1m430	253	60	I2	I2	NNP
cord-104282-90t1m430	253	61	are	be	VBP
cord-104282-90t1m430	253	62	deleted	delete	VBN
cord-104282-90t1m430	253	63	.	.	.
cord-104282-90t1m430	254	1	The	the	DT
cord-104282-90t1m430	254	2	intracellular	intracellular	JJ
cord-104282-90t1m430	254	3	localization	localization	NN
cord-104282-90t1m430	254	4	of	of	IN
cord-104282-90t1m430	254	5	these	these	DT
cord-104282-90t1m430	254	6	chimeras	chimera	NNS
cord-104282-90t1m430	254	7	expressed	express	VBN
cord-104282-90t1m430	254	8	in	in	IN
cord-104282-90t1m430	254	9	COS-1	COS-1	NNP
cord-104282-90t1m430	254	10	cells	cell	NNS
cord-104282-90t1m430	254	11	was	be	VBD
cord-104282-90t1m430	254	12	analyzed	analyze	VBN
cord-104282-90t1m430	254	13	by	by	IN
cord-104282-90t1m430	254	14	indirect	indirect	JJ
cord-104282-90t1m430	254	15	immunofluorescence	immunofluorescence	NN
cord-104282-90t1m430	254	16	microscopy	microscopy	NN
cord-104282-90t1m430	254	17	.	.	.
cord-104282-90t1m430	255	1	Three	three	CD
cord-104282-90t1m430	255	2	chimeras	chimera	NNS
cord-104282-90t1m430	255	3	,	,	,
cord-104282-90t1m430	255	4	CAT	cat	NN
cord-104282-90t1m430	255	5	/	/	SYM
cord-104282-90t1m430	255	6	ALDH5	ALDH5	NNP
cord-104282-90t1m430	255	7	,	,	,
cord-104282-90t1m430	255	8	CAT	CAT	NNP
cord-104282-90t1m430	255	9	/	/	SYM
cord-104282-90t1m430	255	10	ALDH7	aldh7	NN
cord-104282-90t1m430	255	11	,	,	,
cord-104282-90t1m430	255	12	and	and	CC
cord-104282-90t1m430	255	13	CAT	CAT	NNP
cord-104282-90t1m430	255	14	/	/	SYM
cord-104282-90t1m430	255	15	ALDH9	ALDH9	NNP
cord-104282-90t1m430	255	16	,	,	,
cord-104282-90t1m430	255	17	were	be	VBD
cord-104282-90t1m430	255	18	supposed	suppose	VBN
cord-104282-90t1m430	255	19	to	to	TO
cord-104282-90t1m430	255	20	be	be	VB
cord-104282-90t1m430	255	21	directed	direct	VBN
cord-104282-90t1m430	255	22	to	to	IN
cord-104282-90t1m430	255	23	the	the	DT
cord-104282-90t1m430	255	24	ER	ER	NNP
cord-104282-90t1m430	255	25	by	by	IN
cord-104282-90t1m430	255	26	virtue	virtue	NN
cord-104282-90t1m430	255	27	of	of	IN
cord-104282-90t1m430	255	28	the	the	DT
cord-104282-90t1m430	255	29	tail	tail	NN
cord-104282-90t1m430	255	30	region	region	NN
cord-104282-90t1m430	255	31	,	,	,
cord-104282-90t1m430	255	32	even	even	RB
cord-104282-90t1m430	255	33	if	if	IN
cord-104282-90t1m430	255	34	they	-PRON-	PRP
cord-104282-90t1m430	255	35	lack	lack	VBP
cord-104282-90t1m430	255	36	an	an	DT
cord-104282-90t1m430	255	37	ER	er	NN
cord-104282-90t1m430	255	38	targeting	targeting	NN
cord-104282-90t1m430	255	39	sequence	sequence	NN
cord-104282-90t1m430	255	40	within	within	IN
cord-104282-90t1m430	255	41	the	the	DT
cord-104282-90t1m430	255	42	stem	stem	NN
cord-104282-90t1m430	255	43	region	region	NN
cord-104282-90t1m430	255	44	,	,	,
cord-104282-90t1m430	255	45	and	and	CC
cord-104282-90t1m430	255	46	indeed	indeed	RB
cord-104282-90t1m430	255	47	they	-PRON-	PRP
cord-104282-90t1m430	255	48	showed	show	VBD
cord-104282-90t1m430	255	49	the	the	DT
cord-104282-90t1m430	255	50	reticu	reticu	JJ
cord-104282-90t1m430	255	51	~	~	NFP
cord-104282-90t1m430	255	52	lar	lar	NN
cord-104282-90t1m430	255	53	staining	staining	NN
cord-104282-90t1m430	255	54	pattern	pattern	NN
cord-104282-90t1m430	255	55	including	include	VBG
cord-104282-90t1m430	255	56	the	the	DT
cord-104282-90t1m430	255	57	nuclear	nuclear	JJ
cord-104282-90t1m430	255	58	membrane	membrane	NN
cord-104282-90t1m430	255	59	(	(	-LRB-
cord-104282-90t1m430	255	60	Fig	Fig	NNP
cord-104282-90t1m430	255	61	.	.	.
cord-104282-90t1m430	255	62	10	10	CD
cord-104282-90t1m430	255	63	,	,	,
cord-104282-90t1m430	255	64	A	A	NNP
cord-104282-90t1m430	255	65	,	,	,
cord-104282-90t1m430	255	66	C	C	NNP
cord-104282-90t1m430	255	67	,	,	,
cord-104282-90t1m430	255	68	and	and	CC
cord-104282-90t1m430	255	69	E	e	LS
cord-104282-90t1m430	255	70	)	)	-RRB-
cord-104282-90t1m430	255	71	.	.	.
cord-104282-90t1m430	256	1	Among	among	IN
cord-104282-90t1m430	256	2	the	the	DT
cord-104282-90t1m430	256	3	mutant	mutant	JJ
cord-104282-90t1m430	256	4	proteins	protein	NNS
cord-104282-90t1m430	256	5	lacking	lack	VBG
cord-104282-90t1m430	256	6	the	the	DT
cord-104282-90t1m430	256	7	tail	tail	NN
cord-104282-90t1m430	256	8	region	region	NN
cord-104282-90t1m430	256	9	,	,	,
cord-104282-90t1m430	256	10	CAT	cat	NN
cord-104282-90t1m430	256	11	/	/	SYM
cord-104282-90t1m430	256	12	ALDH6	aldh6	NN
cord-104282-90t1m430	256	13	and	and	CC
cord-104282-90t1m430	256	14	CAT	CAT	NNP
cord-104282-90t1m430	256	15	/	/	SYM
cord-104282-90t1m430	256	16	ALDH10	ALDH10	NNP
cord-104282-90t1m430	256	17	showed	show	VBD
cord-104282-90t1m430	256	18	the	the	DT
cord-104282-90t1m430	256	19	reticular	reticular	JJ
cord-104282-90t1m430	256	20	pattern	pattern	NN
cord-104282-90t1m430	256	21	(	(	-LRB-
cord-104282-90t1m430	256	22	Fig	Fig	NNP
cord-104282-90t1m430	256	23	.	.	.
cord-104282-90t1m430	256	24	10	10	CD
cord-104282-90t1m430	256	25	,	,	,
cord-104282-90t1m430	256	26	B	B	NNP
cord-104282-90t1m430	256	27	and	and	CC
cord-104282-90t1m430	256	28	F	F	NNP
cord-104282-90t1m430	256	29	)	)	-RRB-
cord-104282-90t1m430	256	30	.	.	.
cord-104282-90t1m430	257	1	These	these	DT
cord-104282-90t1m430	257	2	reticular	reticular	JJ
cord-104282-90t1m430	257	3	compartments	compartment	NNS
cord-104282-90t1m430	257	4	were	be	VBD
cord-104282-90t1m430	257	5	confirmed	confirm	VBN
cord-104282-90t1m430	257	6	to	to	TO
cord-104282-90t1m430	257	7	be	be	VB
cord-104282-90t1m430	257	8	the	the	DT
cord-104282-90t1m430	257	9	ER	er	NN
cord-104282-90t1m430	257	10	by	by	IN
cord-104282-90t1m430	257	11	colocalization	colocalization	NN
cord-104282-90t1m430	257	12	with	with	IN
cord-104282-90t1m430	257	13	PDI	PDI	NNP
cord-104282-90t1m430	257	14	(	(	-LRB-
cord-104282-90t1m430	257	15	data	datum	NNS
cord-104282-90t1m430	257	16	not	not	RB
cord-104282-90t1m430	257	17	shown	show	VBN
cord-104282-90t1m430	257	18	)	)	-RRB-
cord-104282-90t1m430	257	19	.	.	.
cord-104282-90t1m430	258	1	In	in	IN
cord-104282-90t1m430	258	2	contrast	contrast	NN
cord-104282-90t1m430	258	3	,	,	,
cord-104282-90t1m430	258	4	CAT	CAT	NNP
cord-104282-90t1m430	258	5	/	/	SYM
cord-104282-90t1m430	258	6	ALDH8	ALDH8	NNP
cord-104282-90t1m430	258	7	,	,	,
cord-104282-90t1m430	258	8	which	which	WDT
cord-104282-90t1m430	258	9	lacks	lack	VBZ
cord-104282-90t1m430	258	10	the	the	DT
cord-104282-90t1m430	258	11	Lys	Lys	NNP
cord-104282-90t1m430	258	12	-	-	HYPH
cord-104282-90t1m430	258	13	Gln	Gln	NNP
cord-104282-90t1m430	258	14	-	-	HYPH
cord-104282-90t1m430	258	15	Phe	phe	NN
cord-104282-90t1m430	258	16	sequence	sequence	NN
cord-104282-90t1m430	258	17	(	(	-LRB-
cord-104282-90t1m430	258	18	amino	amino	JJ
cord-104282-90t1m430	258	19	acids	acid	NNS
cord-104282-90t1m430	258	20	457	457	CD
cord-104282-90t1m430	258	21	-	-	SYM
cord-104282-90t1m430	258	22	459	459	CD
cord-104282-90t1m430	258	23	)	)	-RRB-
cord-104282-90t1m430	258	24	within	within	IN
cord-104282-90t1m430	258	25	the	the	DT
cord-104282-90t1m430	258	26	stem	stem	NN
cord-104282-90t1m430	258	27	region	region	NN
cord-104282-90t1m430	258	28	and	and	CC
cord-104282-90t1m430	258	29	the	the	DT
cord-104282-90t1m430	258	30	tail	tail	NN
cord-104282-90t1m430	258	31	region	region	NN
cord-104282-90t1m430	258	32	,	,	,
cord-104282-90t1m430	258	33	was	be	VBD
cord-104282-90t1m430	258	34	found	find	VBN
cord-104282-90t1m430	258	35	distributed	distribute	VBN
cord-104282-90t1m430	258	36	diffusely	diffusely	RB
cord-104282-90t1m430	258	37	throughout	throughout	IN
cord-104282-90t1m430	258	38	the	the	DT
cord-104282-90t1m430	258	39	cytoplasm	cytoplasm	NN
cord-104282-90t1m430	258	40	and	and	CC
cord-104282-90t1m430	258	41	the	the	DT
cord-104282-90t1m430	258	42	nucleoplasm	nucleoplasm	NN
cord-104282-90t1m430	259	1	(	(	-LRB-
cord-104282-90t1m430	259	2	Fig	Fig	NNP
cord-104282-90t1m430	259	3	.	.	.
cord-104282-90t1m430	259	4	10	10	CD
cord-104282-90t1m430	259	5	D	d	NN
cord-104282-90t1m430	259	6	)	)	-RRB-
cord-104282-90t1m430	259	7	.	.	.
cord-104282-90t1m430	260	1	We	-PRON-	PRP
cord-104282-90t1m430	260	2	investigated	investigate	VBD
cord-104282-90t1m430	260	3	in	in	IN
cord-104282-90t1m430	260	4	more	more	JJR
cord-104282-90t1m430	260	5	detail	detail	NN
cord-104282-90t1m430	260	6	the	the	DT
cord-104282-90t1m430	260	7	intracellular	intracellular	JJ
cord-104282-90t1m430	260	8	localization	localization	NN
cord-104282-90t1m430	260	9	of	of	IN
cord-104282-90t1m430	260	10	CAT	cat	NN
cord-104282-90t1m430	260	11	and	and	CC
cord-104282-90t1m430	260	12	CAT	CAT	NNP
cord-104282-90t1m430	260	13	/	/	SYM
cord-104282-90t1m430	260	14	ALDH	ALDH	NNP
cord-104282-90t1m430	260	15	chimeras	chimera	NNS
cord-104282-90t1m430	260	16	in	in	IN
cord-104282-90t1m430	260	17	transfected	transfecte	VBN
cord-104282-90t1m430	260	18	COS-1	COS-1	NNP
cord-104282-90t1m430	260	19	cells	cell	NNS
cord-104282-90t1m430	260	20	using	use	VBG
cord-104282-90t1m430	260	21	an	an	DT
cord-104282-90t1m430	260	22	IgG	igg	NN
cord-104282-90t1m430	260	23	-	-	HYPH
cord-104282-90t1m430	260	24	gold	gold	NNP
cord-104282-90t1m430	260	25	immunoelectron	immunoelectron	NNP
cord-104282-90t1m430	260	26	microscopic	microscopic	JJ
cord-104282-90t1m430	260	27	technique	technique	NN
cord-104282-90t1m430	260	28	on	on	IN
cord-104282-90t1m430	260	29	frozen	frozen	JJ
cord-104282-90t1m430	260	30	ultrathin	ultrathin	JJ
cord-104282-90t1m430	260	31	sections	section	NNS
cord-104282-90t1m430	260	32	.	.	.
cord-104282-90t1m430	261	1	Gold	gold	NN
cord-104282-90t1m430	261	2	particles	particle	NNS
cord-104282-90t1m430	261	3	were	be	VBD
cord-104282-90t1m430	261	4	distributed	distribute	VBN
cord-104282-90t1m430	261	5	in	in	IN
cord-104282-90t1m430	261	6	the	the	DT
cord-104282-90t1m430	261	7	cytoplasm	cytoplasm	NN
cord-104282-90t1m430	261	8	and	and	CC
cord-104282-90t1m430	261	9	in	in	IN
cord-104282-90t1m430	261	10	the	the	DT
cord-104282-90t1m430	261	11	nucleoplasm	nucleoplasm	NN
cord-104282-90t1m430	261	12	in	in	IN
cord-104282-90t1m430	261	13	COS-1	COS-1	NNP
cord-104282-90t1m430	261	14	cells	cell	NNS
cord-104282-90t1m430	261	15	transfected	transfecte	VBN
cord-104282-90t1m430	261	16	with	with	IN
cord-104282-90t1m430	261	17	pMIWCAT	pmiwcat	NN
cord-104282-90t1m430	261	18	(	(	-LRB-
cord-104282-90t1m430	261	19	Fig	Fig	NNP
cord-104282-90t1m430	261	20	.	.	.
cord-104282-90t1m430	261	21	11	11	CD
cord-104282-90t1m430	261	22	A	A	NNP
cord-104282-90t1m430	261	23	)	)	-RRB-
cord-104282-90t1m430	261	24	.	.	.
cord-104282-90t1m430	262	1	A	a	DT
cord-104282-90t1m430	262	2	similar	similar	JJ
cord-104282-90t1m430	262	3	distribution	distribution	NN
cord-104282-90t1m430	262	4	of	of	IN
cord-104282-90t1m430	262	5	gold	gold	NN
cord-104282-90t1m430	262	6	particles	particle	NNS
cord-104282-90t1m430	262	7	was	be	VBD
cord-104282-90t1m430	262	8	observed	observe	VBN
cord-104282-90t1m430	262	9	in	in	IN
cord-104282-90t1m430	262	10	COS-1	COS-1	NNP
cord-104282-90t1m430	262	11	cells	cell	NNS
cord-104282-90t1m430	262	12	expressing	express	VBG
cord-104282-90t1m430	262	13	either	either	CC
cord-104282-90t1m430	262	14	CAT	cat	NN
cord-104282-90t1m430	262	15	/	/	SYM
cord-104282-90t1m430	262	16	ALDH4	ALDH4	NNP
cord-104282-90t1m430	263	1	(	(	-LRB-
cord-104282-90t1m430	263	2	data	datum	NNS
cord-104282-90t1m430	263	3	not	not	RB
cord-104282-90t1m430	263	4	shown	show	VBN
cord-104282-90t1m430	263	5	)	)	-RRB-
cord-104282-90t1m430	263	6	or	or	CC
cord-104282-90t1m430	264	1	CAT/	cat/	JJ
cord-104282-90t1m430	264	2	ALDH8	aldh8	RB
cord-104282-90t1m430	264	3	(	(	-LRB-
cord-104282-90t1m430	264	4	Fig	fig	NN
cord-104282-90t1m430	264	5	.	.	.
cord-104282-90t1m430	264	6	11	11	CD
cord-104282-90t1m430	264	7	D	d	NN
cord-104282-90t1m430	264	8	)	)	-RRB-
cord-104282-90t1m430	264	9	.	.	.
cord-104282-90t1m430	265	1	In	in	IN
cord-104282-90t1m430	265	2	marked	marked	JJ
cord-104282-90t1m430	265	3	contrast	contrast	NN
cord-104282-90t1m430	265	4	,	,	,
cord-104282-90t1m430	265	5	gold	gold	NN
cord-104282-90t1m430	265	6	particles	particle	NNS
cord-104282-90t1m430	265	7	were	be	VBD
cord-104282-90t1m430	265	8	predominantly	predominantly	RB
cord-104282-90t1m430	265	9	detected	detect	VBN
cord-104282-90t1m430	265	10	on	on	IN
cord-104282-90t1m430	265	11	the	the	DT
cord-104282-90t1m430	265	12	cytoplasmic	cytoplasmic	JJ
cord-104282-90t1m430	265	13	surface	surface	NN
cord-104282-90t1m430	265	14	of	of	IN
cord-104282-90t1m430	265	15	the	the	DT
cord-104282-90t1m430	265	16	ER	ER	NNP
cord-104282-90t1m430	265	17	membrane	membrane	NN
cord-104282-90t1m430	265	18	in	in	IN
cord-104282-90t1m430	265	19	COS-1	COS-1	NNP
cord-104282-90t1m430	265	20	cells	cell	NNS
cord-104282-90t1m430	265	21	expressing	express	VBG
cord-104282-90t1m430	265	22	CAT	cat	NN
cord-104282-90t1m430	265	23	/	/	SYM
cord-104282-90t1m430	265	24	ALDH1	ALDH1	NNP
cord-104282-90t1m430	266	1	(	(	-LRB-
cord-104282-90t1m430	266	2	Fig	Fig	NNP
cord-104282-90t1m430	266	3	.	.	.
cord-104282-90t1m430	266	4	11	11	CD
cord-104282-90t1m430	266	5	 	 	_SP
cord-104282-90t1m430	266	6	B	B	NNP
cord-104282-90t1m430	266	7	)	)	-RRB-
cord-104282-90t1m430	267	1	,	,	,
cord-104282-90t1m430	267	2	CAT	CAT	NNP
cord-104282-90t1m430	267	3	/	/	SYM
cord-104282-90t1m430	267	4	ALDH7	aldh7	NN
cord-104282-90t1m430	268	1	(	(	-LRB-
cord-104282-90t1m430	268	2	Fig	Fig	NNP
cord-104282-90t1m430	268	3	.	.	.
cord-104282-90t1m430	268	4	11	11	CD
cord-104282-90t1m430	268	5	C	C	NNP
cord-104282-90t1m430	268	6	)	)	-RRB-
cord-104282-90t1m430	268	7	,	,	,
cord-104282-90t1m430	268	8	or	or	CC
cord-104282-90t1m430	268	9	the	the	DT
cord-104282-90t1m430	268	10	other	other	JJ
cord-104282-90t1m430	268	11	CAT	cat	NN
cord-104282-90t1m430	268	12	/	/	SYM
cord-104282-90t1m430	268	13	ALDH	ALDH	NNP
cord-104282-90t1m430	268	14	chimeras	chimera	NNS
cord-104282-90t1m430	268	15	(	(	-LRB-
cord-104282-90t1m430	268	16	data	datum	NNS
cord-104282-90t1m430	268	17	not	not	RB
cord-104282-90t1m430	268	18	shown	show	VBN
cord-104282-90t1m430	268	19	)	)	-RRB-
cord-104282-90t1m430	268	20	.	.	.
cord-104282-90t1m430	269	1	These	these	DT
cord-104282-90t1m430	269	2	immunoelectron	immunoelectron	NNP
cord-104282-90t1m430	269	3	micro-	micro-	NNP
cord-104282-90t1m430	269	4	scopic	scopic	NNP
cord-104282-90t1m430	269	5	data	data	NNP
cord-104282-90t1m430	269	6	are	be	VBP
cord-104282-90t1m430	269	7	consistent	consistent	JJ
cord-104282-90t1m430	269	8	with	with	IN
cord-104282-90t1m430	269	9	indirect	indirect	JJ
cord-104282-90t1m430	269	10	immunofluorescent	immunofluorescent	NNP
cord-104282-90t1m430	269	11	data	datum	NNS
cord-104282-90t1m430	269	12	,	,	,
cord-104282-90t1m430	269	13	and	and	CC
cord-104282-90t1m430	269	14	these	these	DT
cord-104282-90t1m430	269	15	immunolocalization	immunolocalization	NN
cord-104282-90t1m430	269	16	data	datum	NNS
cord-104282-90t1m430	269	17	together	together	RB
cord-104282-90t1m430	269	18	strongly	strongly	RB
cord-104282-90t1m430	269	19	suggest	suggest	VBP
cord-104282-90t1m430	269	20	that	that	IN
cord-104282-90t1m430	269	21	the	the	DT
cord-104282-90t1m430	269	22	ER	ER	NNP
cord-104282-90t1m430	269	23	-	-	HYPH
cord-104282-90t1m430	269	24	targeting	target	VBG
cord-104282-90t1m430	269	25	sequences	sequence	NNS
cord-104282-90t1m430	269	26	of	of	IN
cord-104282-90t1m430	269	27	msALDH	msALDH	NNS
cord-104282-90t1m430	269	28	exist	exist	VBP
cord-104282-90t1m430	269	29	within	within	IN
cord-104282-90t1m430	269	30	the	the	DT
cord-104282-90t1m430	269	31	Lys	Lys	NNP
cord-104282-90t1m430	269	32	-	-	HYPH
cord-104282-90t1m430	269	33	Gln	Gln	NNP
cord-104282-90t1m430	269	34	-	-	HYPH
cord-104282-90t1m430	269	35	Phe	phe	NN
cord-104282-90t1m430	269	36	sequence	sequence	NN
cord-104282-90t1m430	269	37	in	in	IN
cord-104282-90t1m430	269	38	the	the	DT
cord-104282-90t1m430	269	39	stem	stem	NN
cord-104282-90t1m430	269	40	region	region	NN
cord-104282-90t1m430	269	41	and	and	CC
cord-104282-90t1m430	269	42	within	within	IN
cord-104282-90t1m430	269	43	the	the	DT
cord-104282-90t1m430	269	44	Lys	Lys	NNP
cord-104282-90t1m430	269	45	-	-	HYPH
cord-104282-90t1m430	269	46	Asp	Asp	NNP
cord-104282-90t1m430	269	47	-	-	HYPH
cord-104282-90t1m430	269	48	Gln	Gln	NNP
cord-104282-90t1m430	269	49	-	-	HYPH
cord-104282-90t1m430	269	50	Leu	Leu	NNP
cord-104282-90t1m430	269	51	sequence	sequence	NN
cord-104282-90t1m430	269	52	of	of	IN
cord-104282-90t1m430	269	53	the	the	DT
cord-104282-90t1m430	269	54	tail	tail	NN
cord-104282-90t1m430	269	55	region	region	NN
cord-104282-90t1m430	269	56	.	.	.
cord-104282-90t1m430	270	1	We	-PRON-	PRP
cord-104282-90t1m430	270	2	have	have	VBP
cord-104282-90t1m430	270	3	shown	show	VBN
cord-104282-90t1m430	270	4	here	here	RB
cord-104282-90t1m430	270	5	by	by	IN
cord-104282-90t1m430	270	6	subcellular	subcellular	JJ
cord-104282-90t1m430	270	7	fracfionation	fracfionation	NN
cord-104282-90t1m430	270	8	and	and	CC
cord-104282-90t1m430	270	9	indirect	indirect	JJ
cord-104282-90t1m430	270	10	immunofluorescence	immunofluorescence	NN
cord-104282-90t1m430	270	11	experiments	experiment	NNS
cord-104282-90t1m430	270	12	that	that	WDT
cord-104282-90t1m430	270	13	not	not	RB
cord-104282-90t1m430	270	14	only	only	RB
cord-104282-90t1m430	270	15	msALDH	msALDH	VBN
cord-104282-90t1m430	270	16	,	,	,
cord-104282-90t1m430	270	17	but	but	CC
cord-104282-90t1m430	270	18	also	also	RB
cord-104282-90t1m430	270	19	msALDHA481	msaldha481	CD
cord-104282-90t1m430	270	20	-	-	SYM
cord-104282-90t1m430	270	21	484	484	CD
cord-104282-90t1m430	270	22	,	,	,
cord-104282-90t1m430	270	23	colocalizes	colocalize	VBZ
cord-104282-90t1m430	270	24	with	with	IN
cord-104282-90t1m430	270	25	two	two	CD
cord-104282-90t1m430	270	26	ER	ER	NNP
cord-104282-90t1m430	270	27	marker	marker	NN
cord-104282-90t1m430	270	28	proteins	protein	NNS
cord-104282-90t1m430	270	29	,	,	,
cord-104282-90t1m430	270	30	FP2	FP2	NNS
cord-104282-90t1m430	270	31	and	and	CC
cord-104282-90t1m430	270	32	PDI	PDI	NNP
cord-104282-90t1m430	270	33	,	,	,
cord-104282-90t1m430	270	34	when	when	WRB
cord-104282-90t1m430	270	35	expressed	express	VBN
cord-104282-90t1m430	270	36	from	from	IN
cord-104282-90t1m430	270	37	cDNAs	cDNAs	NNPS
cord-104282-90t1m430	270	38	in	in	IN
cord-104282-90t1m430	270	39	COS-1	COS-1	NNP
cord-104282-90t1m430	270	40	cells	cell	NNS
cord-104282-90t1m430	270	41	.	.	.
cord-104282-90t1m430	271	1	Surprisingly	surprisingly	RB
cord-104282-90t1m430	271	2	,	,	,
cord-104282-90t1m430	271	3	indirect	indirect	JJ
cord-104282-90t1m430	271	4	immunofluorescence	immunofluorescence	NN
cord-104282-90t1m430	271	5	microscopy	microscopy	NN
cord-104282-90t1m430	271	6	revealed	reveal	VBD
cord-104282-90t1m430	271	7	the	the	DT
cord-104282-90t1m430	271	8	apparent	apparent	JJ
cord-104282-90t1m430	271	9	alteration	alteration	NN
cord-104282-90t1m430	271	10	of	of	IN
cord-104282-90t1m430	271	11	the	the	DT
cord-104282-90t1m430	271	12	ER	ER	NNP
cord-104282-90t1m430	271	13	structure	structure	NN
cord-104282-90t1m430	271	14	in	in	IN
cord-104282-90t1m430	271	15	cells	cell	NNS
cord-104282-90t1m430	271	16	expressing	express	VBG
cord-104282-90t1m430	271	17	either	either	CC
cord-104282-90t1m430	271	18	msALDH	msALDH	VBN
cord-104282-90t1m430	271	19	or	or	CC
cord-104282-90t1m430	271	20	msALDH	msALDH	VBN
cord-104282-90t1m430	271	21	-	-	HYPH
cord-104282-90t1m430	271	22	A481	a481	NN
cord-104282-90t1m430	271	23	-	-	HYPH
cord-104282-90t1m430	271	24	484	484	CD
cord-104282-90t1m430	271	25	.	.	.
cord-104282-90t1m430	272	1	The	the	DT
cord-104282-90t1m430	272	2	altered	altered	JJ
cord-104282-90t1m430	272	3	ER	ER	NNP
cord-104282-90t1m430	272	4	structure	structure	NN
cord-104282-90t1m430	272	5	was	be	VBD
cord-104282-90t1m430	272	6	characterized	characterize	VBN
cord-104282-90t1m430	272	7	by	by	IN
cord-104282-90t1m430	272	8	large	large	JJ
cord-104282-90t1m430	272	9	vesicular	vesicular	JJ
cord-104282-90t1m430	272	10	structures	structure	NNS
cord-104282-90t1m430	272	11	mainly	mainly	RB
cord-104282-90t1m430	272	12	located	locate	VBN
cord-104282-90t1m430	272	13	near	near	IN
cord-104282-90t1m430	272	14	the	the	DT
cord-104282-90t1m430	272	15	nucleus	nucleus	NN
cord-104282-90t1m430	272	16	.	.	.
cord-104282-90t1m430	273	1	Since	since	IN
cord-104282-90t1m430	273	2	the	the	DT
cord-104282-90t1m430	273	3	unusual	unusual	JJ
cord-104282-90t1m430	273	4	staining	staining	NN
cord-104282-90t1m430	273	5	pattern	pattern	NN
cord-104282-90t1m430	273	6	was	be	VBD
cord-104282-90t1m430	273	7	observed	observe	VBN
cord-104282-90t1m430	273	8	by	by	IN
cord-104282-90t1m430	273	9	expression	expression	NN
cord-104282-90t1m430	273	10	of	of	IN
cord-104282-90t1m430	273	11	msALDH	msALDH	NNP
cord-104282-90t1m430	273	12	or	or	CC
cord-104282-90t1m430	273	13	msALDHA481.484	msaldha481.484	NN
cord-104282-90t1m430	273	14	,	,	,
cord-104282-90t1m430	273	15	it	-PRON-	PRP
cord-104282-90t1m430	273	16	appeared	appear	VBD
cord-104282-90t1m430	273	17	that	that	IN
cord-104282-90t1m430	273	18	the	the	DT
cord-104282-90t1m430	273	19	ER	ER	NNP
cord-104282-90t1m430	273	20	an-	an-	CC
cord-104282-90t1m430	273	21	choring	choring	NN
cord-104282-90t1m430	273	22	of	of	IN
cord-104282-90t1m430	273	23	these	these	DT
cord-104282-90t1m430	273	24	proteins	protein	NNS
cord-104282-90t1m430	273	25	led	lead	VBD
cord-104282-90t1m430	273	26	to	to	IN
cord-104282-90t1m430	273	27	the	the	DT
cord-104282-90t1m430	273	28	morphological	morphological	JJ
cord-104282-90t1m430	273	29	alteration	alteration	NN
cord-104282-90t1m430	273	30	of	of	IN
cord-104282-90t1m430	273	31	the	the	DT
cord-104282-90t1m430	273	32	ER	ER	NNP
cord-104282-90t1m430	273	33	.	.	.
cord-104282-90t1m430	274	1	We	-PRON-	PRP
cord-104282-90t1m430	274	2	are	be	VBP
cord-104282-90t1m430	274	3	now	now	RB
cord-104282-90t1m430	274	4	investigating	investigate	VBG
cord-104282-90t1m430	274	5	in	in	IN
cord-104282-90t1m430	274	6	more	more	JJR
cord-104282-90t1m430	274	7	detail	detail	NN
cord-104282-90t1m430	274	8	the	the	DT
cord-104282-90t1m430	274	9	altered	alter	VBN
cord-104282-90t1m430	274	10	ER	ER	NNP
cord-104282-90t1m430	274	11	structure	structure	NN
cord-104282-90t1m430	274	12	by	by	IN
cord-104282-90t1m430	274	13	immunoelectron	immunoelectron	NNP
cord-104282-90t1m430	274	14	microscopy	microscopy	NNP
cord-104282-90t1m430	274	15	,	,	,
cord-104282-90t1m430	274	16	and	and	CC
cord-104282-90t1m430	274	17	we	-PRON-	PRP
cord-104282-90t1m430	274	18	have	have	VBP
cord-104282-90t1m430	274	19	recently	recently	RB
cord-104282-90t1m430	274	20	found	find	VBN
cord-104282-90t1m430	274	21	that	that	IN
cord-104282-90t1m430	274	22	the	the	DT
cord-104282-90t1m430	274	23	ER	ER	NNP
cord-104282-90t1m430	274	24	tubules	tubule	NNS
cord-104282-90t1m430	274	25	in	in	IN
cord-104282-90t1m430	274	26	COS-1	COS-1	NNP
cord-104282-90t1m430	274	27	cells	cell	NNS
cord-104282-90t1m430	274	28	expressing	express	VBG
cord-104282-90t1m430	274	29	msALDH	msALDH	NNS
cord-104282-90t1m430	274	30	are	be	VBP
cord-104282-90t1m430	274	31	packed	pack	VBN
cord-104282-90t1m430	274	32	in	in	IN
cord-104282-90t1m430	274	33	crystalloid	crystalloid	JJ
cord-104282-90t1m430	274	34	hexagonal	hexagonal	JJ
cord-104282-90t1m430	274	35	arrays	array	NNS
cord-104282-90t1m430	274	36	(	(	-LRB-
cord-104282-90t1m430	274	37	Yamamoto	Yamamoto	NNP
cord-104282-90t1m430	274	38	,	,	,
cord-104282-90t1m430	274	39	A.	A.	NNP
cord-104282-90t1m430	274	40	,	,	,
cord-104282-90t1m430	274	41	R.	R.	NNP
cord-104282-90t1m430	274	42	Masaki	Masaki	NNP
cord-104282-90t1m430	274	43	,	,	,
cord-104282-90t1m430	274	44	and	and	CC
cord-104282-90t1m430	274	45	Y.	Y.	NNP
cord-104282-90t1m430	274	46	Tashiro	Tashiro	NNP
cord-104282-90t1m430	274	47	,	,	,
cord-104282-90t1m430	274	48	manuscript	manuscript	NN
cord-104282-90t1m430	274	49	in	in	IN
cord-104282-90t1m430	274	50	preparation	preparation	NN
cord-104282-90t1m430	274	51	)	)	-RRB-
cord-104282-90t1m430	274	52	.	.	.
cord-104282-90t1m430	275	1	The	the	DT
cord-104282-90t1m430	275	2	elucidation	elucidation	NN
cord-104282-90t1m430	275	3	of	of	IN
cord-104282-90t1m430	275	4	the	the	DT
cord-104282-90t1m430	275	5	altered	alter	VBN
cord-104282-90t1m430	275	6	ER	ER	NNP
cord-104282-90t1m430	275	7	structure	structure	NN
cord-104282-90t1m430	275	8	would	would	MD
cord-104282-90t1m430	275	9	provide	provide	VB
cord-104282-90t1m430	275	10	interesting	interesting	JJ
cord-104282-90t1m430	275	11	information	information	NN
cord-104282-90t1m430	275	12	on	on	IN
cord-104282-90t1m430	275	13	the	the	DT
cord-104282-90t1m430	275	14	morphological	morphological	JJ
cord-104282-90t1m430	275	15	change	change	NN
cord-104282-90t1m430	275	16	of	of	IN
cord-104282-90t1m430	275	17	the	the	DT
cord-104282-90t1m430	275	18	intracellular	intracellular	JJ
cord-104282-90t1m430	275	19	organelle	organelle	NN
cord-104282-90t1m430	275	20	induced	induce	VBN
cord-104282-90t1m430	275	21	by	by	IN
cord-104282-90t1m430	275	22	overexpression	overexpression	NN
cord-104282-90t1m430	275	23	of	of	IN
cord-104282-90t1m430	275	24	a	a	DT
cord-104282-90t1m430	275	25	resident	resident	JJ
cord-104282-90t1m430	275	26	membrane	membrane	NN
cord-104282-90t1m430	275	27	protein	protein	NN
cord-104282-90t1m430	275	28	.	.	.
cord-104282-90t1m430	276	1	We	-PRON-	PRP
cord-104282-90t1m430	276	2	showed	show	VBD
cord-104282-90t1m430	276	3	previously	previously	RB
cord-104282-90t1m430	276	4	that	that	IN
cord-104282-90t1m430	276	5	msALDH	msALDH	NNP
cord-104282-90t1m430	276	6	has	have	VBZ
cord-104282-90t1m430	276	7	no	no	DT
cord-104282-90t1m430	276	8	amino	amino	NN
cord-104282-90t1m430	276	9	-	-	HYPH
cord-104282-90t1m430	276	10	terminai	terminai	NN
cord-104282-90t1m430	276	11	signal	signal	NN
cord-104282-90t1m430	276	12	sequence	sequence	NN
cord-104282-90t1m430	276	13	,	,	,
cord-104282-90t1m430	276	14	but	but	CC
cord-104282-90t1m430	276	15	instead	instead	RB
cord-104282-90t1m430	276	16	,	,	,
cord-104282-90t1m430	276	17	that	that	IN
cord-104282-90t1m430	276	18	it	-PRON-	PRP
cord-104282-90t1m430	276	19	has	have	VBZ
cord-104282-90t1m430	276	20	a	a	DT
cord-104282-90t1m430	276	21	longer	long	RBR
cord-104282-90t1m430	276	22	carhoxylterminal	carhoxylterminal	JJ
cord-104282-90t1m430	276	23	portion	portion	NN
cord-104282-90t1m430	276	24	than	than	IN
cord-104282-90t1m430	276	25	cytosolic	cytosolic	JJ
cord-104282-90t1m430	276	26	tumor	tumor	NN
cord-104282-90t1m430	276	27	-	-	HYPH
cord-104282-90t1m430	276	28	associated	associate	VBN
cord-104282-90t1m430	276	29	ALDH	ALDH	NNP
cord-104282-90t1m430	276	30	(	(	-LRB-
cord-104282-90t1m430	276	31	24	24	CD
cord-104282-90t1m430	276	32	)	)	-RRB-
cord-104282-90t1m430	276	33	.	.	.
cord-104282-90t1m430	277	1	Our	-PRON-	PRP$
cord-104282-90t1m430	277	2	truncation	truncation	NN
cord-104282-90t1m430	277	3	and	and	CC
cord-104282-90t1m430	277	4	domain	domain	NN
cord-104282-90t1m430	277	5	replacement	replacement	NN
cord-104282-90t1m430	277	6	experiments	experiment	NNS
cord-104282-90t1m430	277	7	have	have	VBP
cord-104282-90t1m430	277	8	shown	show	VBN
cord-104282-90t1m430	277	9	here	here	RB
cord-104282-90t1m430	277	10	that	that	IN
cord-104282-90t1m430	277	11	the	the	DT
cord-104282-90t1m430	277	12	last	last	JJ
cord-104282-90t1m430	277	13	35	35	CD
cord-104282-90t1m430	277	14	amino	amino	NN
cord-104282-90t1m430	277	15	acids	acid	NNS
cord-104282-90t1m430	277	16	of	of	IN
cord-104282-90t1m430	277	17	msALDH	msALDH	NNS
cord-104282-90t1m430	277	18	are	be	VBP
cord-104282-90t1m430	277	19	necessary	necessary	JJ
cord-104282-90t1m430	277	20	for	for	IN
cord-104282-90t1m430	277	21	its	-PRON-	PRP$
cord-104282-90t1m430	277	22	ER	ER	NNP
cord-104282-90t1m430	277	23	localization	localization	NN
cord-104282-90t1m430	277	24	and	and	CC
cord-104282-90t1m430	277	25	also	also	RB
cord-104282-90t1m430	277	26	sufficient	sufficient	JJ
cord-104282-90t1m430	277	27	enough	enough	RB
cord-104282-90t1m430	277	28	for	for	IN
cord-104282-90t1m430	277	29	localization	localization	NN
cord-104282-90t1m430	277	30	of	of	IN
cord-104282-90t1m430	277	31	two	two	CD
cord-104282-90t1m430	277	32	beterologous	beterologous	JJ
cord-104282-90t1m430	277	33	proteins	protein	NNS
cord-104282-90t1m430	277	34	,	,	,
cord-104282-90t1m430	277	35	E.	E.	NNP
cord-104282-90t1m430	277	36	coli	coli	NNS
cord-104282-90t1m430	277	37	/~-galactosidase	/~-galactosidase	NN
cord-104282-90t1m430	277	38	and	and	CC
cord-104282-90t1m430	277	39	CAT	cat	NN
cord-104282-90t1m430	277	40	,	,	,
cord-104282-90t1m430	277	41	to	to	IN
cord-104282-90t1m430	277	42	the	the	DT
cord-104282-90t1m430	277	43	ER	ER	NNP
cord-104282-90t1m430	277	44	.	.	.
cord-104282-90t1m430	278	1	We	-PRON-	PRP
cord-104282-90t1m430	278	2	therefore	therefore	RB
cord-104282-90t1m430	278	3	conclude	conclude	VBP
cord-104282-90t1m430	278	4	that	that	IN
cord-104282-90t1m430	278	5	the	the	DT
cord-104282-90t1m430	278	6	carboxyl	carboxyl	NN
cord-104282-90t1m430	278	7	-	-	HYPH
cord-104282-90t1m430	278	8	terminal	terminal	NN
cord-104282-90t1m430	278	9	portion	portion	NN
cord-104282-90t1m430	278	10	contains	contain	VBZ
cord-104282-90t1m430	278	11	an	an	DT
cord-104282-90t1m430	278	12	ER	ER	NNP
cord-104282-90t1m430	278	13	-	-	HYPH
cord-104282-90t1m430	278	14	targeting	target	VBG
cord-104282-90t1m430	278	15	sequence	sequence	NN
cord-104282-90t1m430	278	16	of	of	IN
cord-104282-90t1m430	278	17	msALDH	msALDH	NNP
cord-104282-90t1m430	278	18	.	.	.
cord-104282-90t1m430	279	1	Further	further	JJ
cord-104282-90t1m430	279	2	analyses	analysis	NNS
cord-104282-90t1m430	279	3	on	on	IN
cord-104282-90t1m430	279	4	the	the	DT
cord-104282-90t1m430	279	5	intraceilular	intraceilular	JJ
cord-104282-90t1m430	279	6	distribution	distribution	NN
cord-104282-90t1m430	279	7	of	of	IN
cord-104282-90t1m430	279	8	various	various	JJ
cord-104282-90t1m430	279	9	CAT	cat	NN
cord-104282-90t1m430	279	10	/	/	SYM
cord-104282-90t1m430	279	11	ALDH	ALDH	NNP
cord-104282-90t1m430	279	12	chimeras	chimera	NNS
cord-104282-90t1m430	279	13	have	have	VBP
cord-104282-90t1m430	279	14	defined	define	VBN
cord-104282-90t1m430	279	15	the	the	DT
cord-104282-90t1m430	279	16	ER	ER	NNP
cord-104282-90t1m430	279	17	targeting	targeting	NN
cord-104282-90t1m430	279	18	sequence	sequence	NN
cord-104282-90t1m430	279	19	ofmsALDH	ofmsALDH	NNP
cord-104282-90t1m430	279	20	.	.	.
cord-104282-90t1m430	280	1	Both	both	DT
cord-104282-90t1m430	280	2	CAT	CAT	NNP
cord-104282-90t1m430	280	3	/	/	SYM
cord-104282-90t1m430	280	4	ALDH4	ALDH4	NNP
cord-104282-90t1m430	280	5	and	and	CC
cord-104282-90t1m430	280	6	CAT	CAT	NNP
cord-104282-90t1m430	281	1	/ALDH8	/ALDH8	:
cord-104282-90t1m430	281	2	were	be	VBD
cord-104282-90t1m430	281	3	distributed	distribute	VBN
cord-104282-90t1m430	281	4	in	in	IN
cord-104282-90t1m430	281	5	the	the	DT
cord-104282-90t1m430	281	6	cytoplasm	cytoplasm	NN
cord-104282-90t1m430	281	7	and	and	CC
cord-104282-90t1m430	281	8	nucleoplasm	nucleoplasm	NN
cord-104282-90t1m430	281	9	similar	similar	JJ
cord-104282-90t1m430	281	10	to	to	IN
cord-104282-90t1m430	281	11	wild	wild	JJ
cord-104282-90t1m430	281	12	-	-	HYPH
cord-104282-90t1m430	281	13	type	type	NN
cord-104282-90t1m430	281	14	CAT	cat	NN
cord-104282-90t1m430	281	15	in	in	IN
cord-104282-90t1m430	281	16	spite	spite	NN
cord-104282-90t1m430	281	17	of	of	IN
cord-104282-90t1m430	281	18	possessing	possess	VBG
cord-104282-90t1m430	281	19	the	the	DT
cord-104282-90t1m430	281	20	whole	whole	JJ
cord-104282-90t1m430	281	21	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	281	22	domain	domain	NN
cord-104282-90t1m430	281	23	(	(	-LRB-
cord-104282-90t1m430	281	24	amino	amino	NN
cord-104282-90t1m430	281	25	acids	acid	NNS
cord-104282-90t1m430	281	26	464	464	CD
cord-104282-90t1m430	281	27	-	-	SYM
cord-104282-90t1m430	281	28	480	480	CD
cord-104282-90t1m430	281	29	)	)	-RRB-
cord-104282-90t1m430	281	30	of	of	IN
cord-104282-90t1m430	281	31	msALDH	msALDH	NNP
cord-104282-90t1m430	281	32	at	at	IN
cord-104282-90t1m430	281	33	their	-PRON-	PRP$
cord-104282-90t1m430	281	34	carboxyl	carboxyl	NN
cord-104282-90t1m430	281	35	termini	terminus	NNS
cord-104282-90t1m430	281	36	,	,	,
cord-104282-90t1m430	281	37	whereas	whereas	IN
cord-104282-90t1m430	281	38	the	the	DT
cord-104282-90t1m430	281	39	other	other	JJ
cord-104282-90t1m430	281	40	chimeras	chimera	NNS
cord-104282-90t1m430	281	41	were	be	VBD
cord-104282-90t1m430	281	42	predominantly	predominantly	RB
cord-104282-90t1m430	281	43	localized	localize	VBN
cord-104282-90t1m430	281	44	to	to	IN
cord-104282-90t1m430	281	45	the	the	DT
cord-104282-90t1m430	281	46	ER	ER	NNP
cord-104282-90t1m430	281	47	.	.	.
cord-104282-90t1m430	282	1	The	the	DT
cord-104282-90t1m430	282	2	two	two	CD
cord-104282-90t1m430	282	3	chimeras	chimera	NNS
cord-104282-90t1m430	282	4	are	be	VBP
cord-104282-90t1m430	282	5	lacking	lack	VBG
cord-104282-90t1m430	282	6	the	the	DT
cord-104282-90t1m430	282	7	Lys	Lys	NNP
cord-104282-90t1m430	282	8	-	-	HYPH
cord-104282-90t1m430	282	9	Gln	Gln	NNP
cord-104282-90t1m430	282	10	-	-	HYPH
cord-104282-90t1m430	282	11	Phe	phe	NN
cord-104282-90t1m430	282	12	sequence	sequence	NN
cord-104282-90t1m430	282	13	within	within	IN
cord-104282-90t1m430	282	14	the	the	DT
cord-104282-90t1m430	282	15	stem	stem	NN
cord-104282-90t1m430	282	16	region	region	NN
cord-104282-90t1m430	282	17	and	and	CC
cord-104282-90t1m430	282	18	the	the	DT
cord-104282-90t1m430	282	19	Lys	Lys	NNP
cord-104282-90t1m430	282	20	-	-	HYPH
cord-104282-90t1m430	282	21	Asp	Asp	NNP
cord-104282-90t1m430	282	22	-	-	HYPH
cord-104282-90t1m430	282	23	Gln	Gln	NNP
cord-104282-90t1m430	282	24	-	-	HYPH
cord-104282-90t1m430	282	25	Leu	Leu	NNP
cord-104282-90t1m430	282	26	sequence	sequence	NN
cord-104282-90t1m430	282	27	of	of	IN
cord-104282-90t1m430	282	28	the	the	DT
cord-104282-90t1m430	282	29	tail	tail	NN
cord-104282-90t1m430	282	30	region	region	NN
cord-104282-90t1m430	282	31	,	,	,
cord-104282-90t1m430	282	32	thus	thus	RB
cord-104282-90t1m430	282	33	suggesting	suggest	VBG
cord-104282-90t1m430	282	34	that	that	IN
cord-104282-90t1m430	282	35	both	both	DT
cord-104282-90t1m430	282	36	sequences	sequence	NNS
cord-104282-90t1m430	282	37	represent	represent	VBP
cord-104282-90t1m430	282	38	the	the	DT
cord-104282-90t1m430	282	39	ER	ER	NNP
cord-104282-90t1m430	282	40	-	-	HYPH
cord-104282-90t1m430	282	41	targeting	target	VBG
cord-104282-90t1m430	282	42	sequences	sequence	NNS
cord-104282-90t1m430	282	43	of	of	IN
cord-104282-90t1m430	282	44	msALDH	msALDH	NNP
cord-104282-90t1m430	282	45	.	.	.
cord-104282-90t1m430	283	1	We	-PRON-	PRP
cord-104282-90t1m430	283	2	are	be	VBP
cord-104282-90t1m430	283	3	now	now	RB
cord-104282-90t1m430	283	4	in	in	IN
cord-104282-90t1m430	283	5	the	the	DT
cord-104282-90t1m430	283	6	process	process	NN
cord-104282-90t1m430	283	7	of	of	IN
cord-104282-90t1m430	283	8	determining	determine	VBG
cord-104282-90t1m430	283	9	through	through	IN
cord-104282-90t1m430	283	10	mutagenesis	mutagenesis	NN
cord-104282-90t1m430	283	11	which	which	WDT
cord-104282-90t1m430	283	12	amino	amino	VBZ
cord-104282-90t1m430	283	13	acids	acid	NNS
cord-104282-90t1m430	283	14	within	within	IN
cord-104282-90t1m430	283	15	these	these	DT
cord-104282-90t1m430	283	16	two	two	CD
cord-104282-90t1m430	283	17	regions	region	NNS
cord-104282-90t1m430	283	18	are	be	VBP
cord-104282-90t1m430	283	19	required	require	VBN
cord-104282-90t1m430	283	20	for	for	IN
cord-104282-90t1m430	283	21	ER	ER	NNP
cord-104282-90t1m430	283	22	targeting	targeting	NN
cord-104282-90t1m430	283	23	.	.	.
cord-104282-90t1m430	284	1	On	on	IN
cord-104282-90t1m430	284	2	the	the	DT
cord-104282-90t1m430	284	3	other	other	JJ
cord-104282-90t1m430	284	4	hand	hand	NN
cord-104282-90t1m430	284	5	,	,	,
cord-104282-90t1m430	284	6	it	-PRON-	PRP
cord-104282-90t1m430	284	7	seems	seem	VBZ
cord-104282-90t1m430	284	8	likely	likely	JJ
cord-104282-90t1m430	284	9	that	that	IN
cord-104282-90t1m430	284	10	the	the	DT
cord-104282-90t1m430	284	11	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	284	12	sequence	sequence	NN
cord-104282-90t1m430	284	13	of	of	IN
cord-104282-90t1m430	284	14	msALDH	msALDH	NNP
cord-104282-90t1m430	284	15	functions	function	NNS
cord-104282-90t1m430	284	16	mainly	mainly	RB
cord-104282-90t1m430	284	17	as	as	IN
cord-104282-90t1m430	284	18	the	the	DT
cord-104282-90t1m430	284	19	ER	ER	NNP
cord-104282-90t1m430	284	20	membrane	membrane	NN
cord-104282-90t1m430	284	21	anchor	anchor	NN
cord-104282-90t1m430	284	22	because	because	IN
cord-104282-90t1m430	284	23	this	this	DT
cord-104282-90t1m430	284	24	portion	portion	NN
cord-104282-90t1m430	284	25	is	be	VBZ
cord-104282-90t1m430	284	26	essential	essential	JJ
cord-104282-90t1m430	284	27	for	for	IN
cord-104282-90t1m430	284	28	membrane	membrane	NN
cord-104282-90t1m430	284	29	anchoring	anchoring	NN
cord-104282-90t1m430	284	30	.	.	.
cord-104282-90t1m430	285	1	To	to	IN
cord-104282-90t1m430	285	2	the	the	DT
cord-104282-90t1m430	285	3	best	good	JJS
cord-104282-90t1m430	285	4	of	of	IN
cord-104282-90t1m430	285	5	our	-PRON-	PRP$
cord-104282-90t1m430	285	6	knowledge	knowledge	NN
cord-104282-90t1m430	285	7	,	,	,
cord-104282-90t1m430	285	8	our	-PRON-	PRP$
cord-104282-90t1m430	285	9	data	datum	NNS
cord-104282-90t1m430	285	10	characterize	characterize	VBP
cord-104282-90t1m430	285	11	the	the	DT
cord-104282-90t1m430	285	12	first	first	JJ
cord-104282-90t1m430	285	13	intracellular	intracellular	JJ
cord-104282-90t1m430	285	14	protein	protein	NN
cord-104282-90t1m430	285	15	that	that	WDT
cord-104282-90t1m430	285	16	appears	appear	VBZ
cord-104282-90t1m430	285	17	to	to	TO
cord-104282-90t1m430	285	18	have	have	VB
cord-104282-90t1m430	285	19	two	two	CD
cord-104282-90t1m430	285	20	targeting	target	VBG
cord-104282-90t1m430	285	21	sequences	sequence	NNS
cord-104282-90t1m430	285	22	separated	separate	VBN
cord-104282-90t1m430	285	23	by	by	IN
cord-104282-90t1m430	285	24	a	a	DT
cord-104282-90t1m430	285	25	membrane	membrane	NN
cord-104282-90t1m430	285	26	anchor	anchor	NN
cord-104282-90t1m430	285	27	.	.	.
cord-104282-90t1m430	286	1	The	the	DT
cord-104282-90t1m430	286	2	ambiguous	ambiguous	JJ
cord-104282-90t1m430	286	3	behavior	behavior	NN
cord-104282-90t1m430	286	4	of	of	IN
cord-104282-90t1m430	286	5	msALDHA471	msaldha471	NN
cord-104282-90t1m430	286	6	-	-	HYPH
cord-104282-90t1m430	286	7	484	484	CD
cord-104282-90t1m430	286	8	could	could	MD
cord-104282-90t1m430	286	9	be	be	VB
cord-104282-90t1m430	286	10	explained	explain	VBN
cord-104282-90t1m430	286	11	as	as	IN
cord-104282-90t1m430	286	12	follows	follow	VBZ
cord-104282-90t1m430	286	13	.	.	.
cord-104282-90t1m430	287	1	A	a	DT
cord-104282-90t1m430	287	2	portion	portion	NN
cord-104282-90t1m430	287	3	of	of	IN
cord-104282-90t1m430	287	4	this	this	DT
cord-104282-90t1m430	287	5	truncated	truncated	JJ
cord-104282-90t1m430	287	6	protein	protein	NN
cord-104282-90t1m430	287	7	could	could	MD
cord-104282-90t1m430	287	8	be	be	VB
cord-104282-90t1m430	287	9	targeted	target	VBN
cord-104282-90t1m430	287	10	to	to	IN
cord-104282-90t1m430	287	11	the	the	DT
cord-104282-90t1m430	287	12	ER	ER	NNP
cord-104282-90t1m430	287	13	by	by	IN
cord-104282-90t1m430	287	14	virtue	virtue	NN
cord-104282-90t1m430	287	15	of	of	IN
cord-104282-90t1m430	287	16	the	the	DT
cord-104282-90t1m430	287	17	Lys	Lys	NNP
cord-104282-90t1m430	287	18	-	-	HYPH
cord-104282-90t1m430	287	19	Gln	Gln	NNP
cord-104282-90t1m430	287	20	-	-	HYPH
cord-104282-90t1m430	287	21	Phe	phe	NN
cord-104282-90t1m430	287	22	sequence	sequence	NN
cord-104282-90t1m430	287	23	within	within	IN
cord-104282-90t1m430	287	24	the	the	DT
cord-104282-90t1m430	287	25	stem	stem	NN
cord-104282-90t1m430	287	26	region	region	NN
cord-104282-90t1m430	287	27	,	,	,
cord-104282-90t1m430	287	28	hut	hut	NN
cord-104282-90t1m430	287	29	because	because	IN
cord-104282-90t1m430	287	30	of	of	IN
cord-104282-90t1m430	287	31	the	the	DT
cord-104282-90t1m430	287	32	shortened	shorten	VBN
cord-104282-90t1m430	287	33	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	287	34	domain	domain	NN
cord-104282-90t1m430	287	35	,	,	,
cord-104282-90t1m430	287	36	the	the	DT
cord-104282-90t1m430	287	37	portion	portion	NN
cord-104282-90t1m430	287	38	would	would	MD
cord-104282-90t1m430	287	39	he	-PRON-	PRP
cord-104282-90t1m430	287	40	either	either	RB
cord-104282-90t1m430	287	41	released	release	VBN
cord-104282-90t1m430	287	42	into	into	IN
cord-104282-90t1m430	287	43	the	the	DT
cord-104282-90t1m430	287	44	cytosol	cytosol	NN
cord-104282-90t1m430	287	45	or	or	CC
cord-104282-90t1m430	287	46	maintain	maintain	VB
cord-104282-90t1m430	287	47	only	only	RB
cord-104282-90t1m430	287	48	a	a	DT
cord-104282-90t1m430	287	49	loose	loose	JJ
cord-104282-90t1m430	287	50	association	association	NN
cord-104282-90t1m430	287	51	with	with	IN
cord-104282-90t1m430	287	52	the	the	DT
cord-104282-90t1m430	287	53	membrane	membrane	NN
cord-104282-90t1m430	287	54	.	.	.
cord-104282-90t1m430	288	1	The	the	DT
cord-104282-90t1m430	288	2	significance	significance	NN
cord-104282-90t1m430	288	3	of	of	IN
cord-104282-90t1m430	288	4	the	the	DT
cord-104282-90t1m430	288	5	presence	presence	NN
cord-104282-90t1m430	288	6	of	of	IN
cord-104282-90t1m430	288	7	two	two	CD
cord-104282-90t1m430	288	8	separated	separate	VBN
cord-104282-90t1m430	288	9	targeting	targeting	NN
cord-104282-90t1m430	288	10	sequences	sequence	NNS
cord-104282-90t1m430	288	11	is	be	VBZ
cord-104282-90t1m430	288	12	not	not	RB
cord-104282-90t1m430	288	13	yet	yet	RB
cord-104282-90t1m430	288	14	clear	clear	JJ
cord-104282-90t1m430	288	15	.	.	.
cord-104282-90t1m430	289	1	A	a	DT
cord-104282-90t1m430	289	2	small	small	JJ
cord-104282-90t1m430	289	3	portion	portion	NN
cord-104282-90t1m430	289	4	of	of	IN
cord-104282-90t1m430	289	5	msALDHA481	msALDHA481	NNP
cord-104282-90t1m430	289	6	-	-	SYM
cord-104282-90t1m430	289	7	484	484	CD
cord-104282-90t1m430	289	8	was	be	VBD
cord-104282-90t1m430	289	9	,	,	,
cord-104282-90t1m430	289	10	in	in	IN
cord-104282-90t1m430	289	11	fact	fact	NN
cord-104282-90t1m430	289	12	,	,	,
cord-104282-90t1m430	289	13	recovered	recover	VBN
cord-104282-90t1m430	289	14	in	in	IN
cord-104282-90t1m430	289	15	the	the	DT
cord-104282-90t1m430	289	16	$	$	$
cord-104282-90t1m430	289	17	3	3	CD
cord-104282-90t1m430	289	18	fraction	fraction	NN
cord-104282-90t1m430	289	19	,	,	,
cord-104282-90t1m430	289	20	whereas	whereas	IN
cord-104282-90t1m430	289	21	only	only	RB
cord-104282-90t1m430	289	22	insignificant	insignificant	JJ
cord-104282-90t1m430	289	23	amounts	amount	NNS
cord-104282-90t1m430	289	24	of	of	IN
cord-104282-90t1m430	289	25	msALDH	msALDH	NNS
cord-104282-90t1m430	289	26	or	or	CC
cord-104282-90t1m430	289	27	FP2	FP2	NNS
cord-104282-90t1m430	289	28	(	(	-LRB-
cord-104282-90t1m430	289	29	Fig	Fig	NNP
cord-104282-90t1m430	289	30	.	.	NNP
cord-104282-90t1m430	289	31	2	2	CD
cord-104282-90t1m430	289	32	,	,	,
cord-104282-90t1m430	289	33	B	b	NN
cord-104282-90t1m430	289	34	and	and	CC
cord-104282-90t1m430	289	35	C	C	NNP
cord-104282-90t1m430	289	36	)	)	-RRB-
cord-104282-90t1m430	289	37	,	,	,
cord-104282-90t1m430	289	38	which	which	WDT
cord-104282-90t1m430	289	39	is	be	VBZ
cord-104282-90t1m430	289	40	efficiently	efficiently	RB
cord-104282-90t1m430	289	41	targeted	target	VBN
cord-104282-90t1m430	289	42	to	to	IN
cord-104282-90t1m430	289	43	the	the	DT
cord-104282-90t1m430	289	44	ER	ER	NNP
cord-104282-90t1m430	289	45	through	through	IN
cord-104282-90t1m430	289	46	the	the	DT
cord-104282-90t1m430	289	47	aminoterminal	aminoterminal	JJ
cord-104282-90t1m430	289	48	signal	signal	NN
cord-104282-90t1m430	289	49	sequence	sequence	NN
cord-104282-90t1m430	289	50	,	,	,
cord-104282-90t1m430	289	51	were	be	VBD
cord-104282-90t1m430	289	52	recovered	recover	VBN
cord-104282-90t1m430	289	53	in	in	IN
cord-104282-90t1m430	289	54	the	the	DT
cord-104282-90t1m430	289	55	$	$	$
cord-104282-90t1m430	289	56	3	3	CD
cord-104282-90t1m430	289	57	fraction	fraction	NN
cord-104282-90t1m430	289	58	.	.	.
cord-104282-90t1m430	290	1	Therefore	therefore	RB
cord-104282-90t1m430	290	2	,	,	,
cord-104282-90t1m430	290	3	it	-PRON-	PRP
cord-104282-90t1m430	290	4	is	be	VBZ
cord-104282-90t1m430	290	5	likely	likely	JJ
cord-104282-90t1m430	290	6	that	that	IN
cord-104282-90t1m430	290	7	the	the	DT
cord-104282-90t1m430	290	8	presence	presence	NN
cord-104282-90t1m430	290	9	of	of	IN
cord-104282-90t1m430	290	10	two	two	CD
cord-104282-90t1m430	290	11	targeting	target	VBG
cord-104282-90t1m430	290	12	sequences	sequence	NNS
cord-104282-90t1m430	290	13	in	in	IN
cord-104282-90t1m430	290	14	the	the	DT
cord-104282-90t1m430	290	15	same	same	JJ
cord-104282-90t1m430	290	16	protein	protein	NN
cord-104282-90t1m430	290	17	might	may	MD
cord-104282-90t1m430	290	18	be	be	VB
cord-104282-90t1m430	290	19	a	a	DT
cord-104282-90t1m430	290	20	means	means	NN
cord-104282-90t1m430	290	21	to	to	TO
cord-104282-90t1m430	290	22	ensure	ensure	VB
cord-104282-90t1m430	290	23	the	the	DT
cord-104282-90t1m430	290	24	more	more	RBR
cord-104282-90t1m430	290	25	efficient	efficient	JJ
cord-104282-90t1m430	290	26	ER	er	NN
cord-104282-90t1m430	290	27	localization	localization	NN
cord-104282-90t1m430	290	28	of	of	IN
cord-104282-90t1m430	290	29	msALDH	msALDH	NNP
cord-104282-90t1m430	290	30	.	.	.
cord-104282-90t1m430	291	1	In	in	IN
cord-104282-90t1m430	291	2	addition	addition	NN
cord-104282-90t1m430	291	3	,	,	,
cord-104282-90t1m430	291	4	the	the	DT
cord-104282-90t1m430	291	5	hydrophilic	hydrophilic	JJ
cord-104282-90t1m430	291	6	tail	tail	NN
cord-104282-90t1m430	291	7	region	region	NN
cord-104282-90t1m430	291	8	might	may	MD
cord-104282-90t1m430	291	9	also	also	RB
cord-104282-90t1m430	291	10	serve	serve	VB
cord-104282-90t1m430	291	11	to	to	TO
cord-104282-90t1m430	291	12	increase	increase	VB
cord-104282-90t1m430	291	13	the	the	DT
cord-104282-90t1m430	291	14	strength	strength	NN
cord-104282-90t1m430	291	15	of	of	IN
cord-104282-90t1m430	291	16	the	the	DT
cord-104282-90t1m430	291	17	membrane	membrane	NN
cord-104282-90t1m430	291	18	anchoring	anchor	VBG
cord-104282-90t1m430	291	19	,	,	,
cord-104282-90t1m430	291	20	as	as	IN
cord-104282-90t1m430	291	21	indicated	indicate	VBN
cord-104282-90t1m430	291	22	by	by	IN
cord-104282-90t1m430	291	23	the	the	DT
cord-104282-90t1m430	291	24	partial	partial	JJ
cord-104282-90t1m430	291	25	membrane	membrane	NN
cord-104282-90t1m430	291	26	extraction	extraction	NN
cord-104282-90t1m430	291	27	of	of	IN
cord-104282-90t1m430	291	28	msALDHA481	msALDHA481	NNP
cord-104282-90t1m430	291	29	-	-	SYM
cord-104282-90t1m430	291	30	484	484	CD
cord-104282-90t1m430	291	31	with	with	IN
cord-104282-90t1m430	291	32	Na2CO3	Na2CO3	NNP
cord-104282-90t1m430	292	1	(	(	-LRB-
cord-104282-90t1m430	292	2	Fig	Fig	NNP
cord-104282-90t1m430	292	3	.	.	.
cord-104282-90t1m430	292	4	3	3	CD
cord-104282-90t1m430	292	5	)	)	-RRB-
cord-104282-90t1m430	292	6	.	.	.
cord-104282-90t1m430	293	1	Although	although	IN
cord-104282-90t1m430	293	2	it	-PRON-	PRP
cord-104282-90t1m430	293	3	is	be	VBZ
cord-104282-90t1m430	293	4	not	not	RB
cord-104282-90t1m430	293	5	yet	yet	RB
cord-104282-90t1m430	293	6	clear	clear	JJ
cord-104282-90t1m430	293	7	whether	whether	IN
cord-104282-90t1m430	293	8	the	the	DT
cord-104282-90t1m430	293	9	(	(	-LRB-
cord-104282-90t1m430	293	10	24	24	CD
cord-104282-90t1m430	293	11	)	)	-RRB-
cord-104282-90t1m430	293	12	.	.	.
cord-104282-90t1m430	294	1	The	the	DT
cord-104282-90t1m430	294	2	single	single	JJ
cord-104282-90t1m430	294	3	amino	amino	NN
cord-104282-90t1m430	294	4	acid	acid	NN
cord-104282-90t1m430	294	5	code	code	NN
cord-104282-90t1m430	294	6	is	be	VBZ
cord-104282-90t1m430	294	7	used	use	VBN
cord-104282-90t1m430	294	8	.	.	.
cord-104282-90t1m430	295	1	Positively	positively	RB
cord-104282-90t1m430	295	2	and	and	CC
cord-104282-90t1m430	295	3	negatively	negatively	RB
cord-104282-90t1m430	295	4	charged	charge	VBN
cord-104282-90t1m430	295	5	amino	amino	NN
cord-104282-90t1m430	295	6	acids	acid	NNS
cord-104282-90t1m430	295	7	are	be	VBP
cord-104282-90t1m430	295	8	marked	mark	VBN
cord-104282-90t1m430	295	9	+	+	.
cord-104282-90t1m430	296	1	and	and	CC
cord-104282-90t1m430	296	2	-at	-at	NFP
cord-104282-90t1m430	296	3	the	the	DT
cord-104282-90t1m430	296	4	top	top	NN
cord-104282-90t1m430	296	5	,	,	,
cord-104282-90t1m430	296	6	respectively	respectively	RB
cord-104282-90t1m430	296	7	.	.	.
cord-104282-90t1m430	297	1	Each	each	DT
cord-104282-90t1m430	297	2	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	297	3	domain	domain	NN
cord-104282-90t1m430	297	4	is	be	VBZ
cord-104282-90t1m430	297	5	underlined	underline	VBN
cord-104282-90t1m430	297	6	and	and	CC
cord-104282-90t1m430	297	7	the	the	DT
cord-104282-90t1m430	297	8	ER	ER	NNP
cord-104282-90t1m430	297	9	targeting	targeting	NN
cord-104282-90t1m430	297	10	sequences	sequence	NNS
cord-104282-90t1m430	297	11	of	of	IN
cord-104282-90t1m430	297	12	either	either	DT
cord-104282-90t1m430	297	13	cytochrome	cytochrome	NN
cord-104282-90t1m430	297	14	b5	b5	NN
cord-104282-90t1m430	297	15	(	(	-LRB-
cord-104282-90t1m430	297	16	23	23	CD
cord-104282-90t1m430	297	17	)	)	-RRB-
cord-104282-90t1m430	297	18	or	or	CC
cord-104282-90t1m430	297	19	msALDH	msALDH	NNP
cord-104282-90t1m430	297	20	are	be	VBP
cord-104282-90t1m430	297	21	indicated	indicate	VBN
cord-104282-90t1m430	297	22	by	by	IN
cord-104282-90t1m430	297	23	broken	broken	JJ
cord-104282-90t1m430	297	24	lines	line	NNS
cord-104282-90t1m430	297	25	.	.	.
cord-104282-90t1m430	298	1	(	(	-LRB-
cord-104282-90t1m430	298	2	B	b	NN
cord-104282-90t1m430	298	3	)	)	-RRB-
cord-104282-90t1m430	298	4	Two	two	CD
cord-104282-90t1m430	298	5	possible	possible	JJ
cord-104282-90t1m430	298	6	models	model	NNS
cord-104282-90t1m430	298	7	for	for	IN
cord-104282-90t1m430	298	8	ER	ER	NNP
cord-104282-90t1m430	298	9	targeting	targeting	NN
cord-104282-90t1m430	298	10	of	of	IN
cord-104282-90t1m430	298	11	msALDH	msALDH	NNP
cord-104282-90t1m430	298	12	.	.	.
cord-104282-90t1m430	299	1	In	in	IN
cord-104282-90t1m430	299	2	model	model	NN
cord-104282-90t1m430	299	3	I	-PRON-	PRP
cord-104282-90t1m430	299	4	,	,	,
cord-104282-90t1m430	299	5	the	the	DT
cord-104282-90t1m430	299	6	aggregation	aggregation	NN
cord-104282-90t1m430	299	7	of	of	IN
cord-104282-90t1m430	299	8	the	the	DT
cord-104282-90t1m430	299	9	newly	newly	RB
cord-104282-90t1m430	299	10	synthesized	synthesize	VBN
cord-104282-90t1m430	299	11	msALDH	msaldh	NN
cord-104282-90t1m430	299	12	polypeptides	polypeptide	NNS
cord-104282-90t1m430	299	13	in	in	IN
cord-104282-90t1m430	299	14	the	the	DT
cord-104282-90t1m430	299	15	cytoplasm	cytoplasm	NN
cord-104282-90t1m430	299	16	is	be	VBZ
cord-104282-90t1m430	299	17	prevented	prevent	VBN
cord-104282-90t1m430	299	18	by	by	IN
cord-104282-90t1m430	299	19	the	the	DT
cord-104282-90t1m430	299	20	hydrophilic	hydrophilic	JJ
cord-104282-90t1m430	299	21	ER	er	NN
cord-104282-90t1m430	299	22	targeting	target	VBG
cord-104282-90t1m430	299	23	sequences	sequence	NNS
cord-104282-90t1m430	299	24	adjacent	adjacent	JJ
cord-104282-90t1m430	299	25	to	to	IN
cord-104282-90t1m430	299	26	the	the	DT
cord-104282-90t1m430	299	27	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	299	28	anchoring	anchoring	NN
cord-104282-90t1m430	299	29	sequence	sequence	NN
cord-104282-90t1m430	299	30	.	.	.
cord-104282-90t1m430	300	1	The	the	DT
cord-104282-90t1m430	300	2	monomeric	monomeric	JJ
cord-104282-90t1m430	300	3	msALDH	msaldh	NN
cord-104282-90t1m430	300	4	would	would	MD
cord-104282-90t1m430	300	5	be	be	VB
cord-104282-90t1m430	300	6	inserted	insert	VBN
cord-104282-90t1m430	300	7	into	into	IN
cord-104282-90t1m430	300	8	the	the	DT
cord-104282-90t1m430	300	9	ER	ER	NNP
cord-104282-90t1m430	300	10	membrane	membrane	NN
cord-104282-90t1m430	300	11	with	with	IN
cord-104282-90t1m430	300	12	the	the	DT
cord-104282-90t1m430	300	13	aid	aid	NN
cord-104282-90t1m430	300	14	of	of	IN
cord-104282-90t1m430	300	15	a	a	DT
cord-104282-90t1m430	300	16	receptor	receptor	NN
cord-104282-90t1m430	300	17	protein	protein	NN
cord-104282-90t1m430	300	18	on	on	IN
cord-104282-90t1m430	300	19	the	the	DT
cord-104282-90t1m430	300	20	ER	ER	NNP
cord-104282-90t1m430	300	21	membrane	membrane	NN
cord-104282-90t1m430	300	22	.	.	.
cord-104282-90t1m430	301	1	In	in	IN
cord-104282-90t1m430	301	2	model	model	NN
cord-104282-90t1m430	301	3	II	II	NNP
cord-104282-90t1m430	301	4	,	,	,
cord-104282-90t1m430	301	5	the	the	DT
cord-104282-90t1m430	301	6	complex	complex	NN
cord-104282-90t1m430	301	7	of	of	IN
cord-104282-90t1m430	301	8	msALDH	msALDH	NNP
cord-104282-90t1m430	301	9	and	and	CC
cord-104282-90t1m430	301	10	a	a	DT
cord-104282-90t1m430	301	11	cytosolic	cytosolic	NN
cord-104282-90t1m430	302	1	•	•	NNP
cord-104282-90t1m430	303	1	receptor	receptor	NN
cord-104282-90t1m430	303	2	protein	protein	NN
cord-104282-90t1m430	303	3	would	would	MD
cord-104282-90t1m430	303	4	be	be	VB
cord-104282-90t1m430	303	5	targeted	target	VBN
cord-104282-90t1m430	303	6	to	to	IN
cord-104282-90t1m430	303	7	the	the	DT
cord-104282-90t1m430	303	8	ER	ER	NNP
cord-104282-90t1m430	303	9	,	,	,
cord-104282-90t1m430	303	10	and	and	CC
cord-104282-90t1m430	303	11	msALDH	msALDH	NNP
cord-104282-90t1m430	303	12	would	would	MD
cord-104282-90t1m430	303	13	be	be	VB
cord-104282-90t1m430	303	14	inserted	insert	VBN
cord-104282-90t1m430	303	15	correctly	correctly	RB
cord-104282-90t1m430	303	16	into	into	IN
cord-104282-90t1m430	303	17	the	the	DT
cord-104282-90t1m430	303	18	ER	ER	NNP
cord-104282-90t1m430	303	19	membrane	membrane	NN
cord-104282-90t1m430	303	20	spontaneously	spontaneously	RB
cord-104282-90t1m430	303	21	or	or	CC
cord-104282-90t1m430	303	22	through	through	IN
cord-104282-90t1m430	303	23	the	the	DT
cord-104282-90t1m430	303	24	interaction	interaction	NN
cord-104282-90t1m430	303	25	with	with	IN
cord-104282-90t1m430	303	26	a	a	DT
cord-104282-90t1m430	303	27	receptor	receptor	NN
cord-104282-90t1m430	303	28	protein	protein	NN
cord-104282-90t1m430	303	29	on	on	IN
cord-104282-90t1m430	303	30	the	the	DT
cord-104282-90t1m430	303	31	membrane	membrane	NN
cord-104282-90t1m430	303	32	.	.	.
cord-104282-90t1m430	304	1	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	304	2	segment	segment	NN
cord-104282-90t1m430	304	3	fully	fully	RB
cord-104282-90t1m430	304	4	transverses	transverse	VBZ
cord-104282-90t1m430	304	5	the	the	DT
cord-104282-90t1m430	304	6	membrane	membrane	NN
cord-104282-90t1m430	304	7	,	,	,
cord-104282-90t1m430	304	8	this	this	DT
cord-104282-90t1m430	304	9	transmembrane	transmembrane	NN
cord-104282-90t1m430	304	10	disposition	disposition	NN
cord-104282-90t1m430	304	11	seems	seem	VBZ
cord-104282-90t1m430	304	12	more	more	RBR
cord-104282-90t1m430	304	13	likely	likely	JJ
cord-104282-90t1m430	304	14	to	to	TO
cord-104282-90t1m430	304	15	explain	explain	VB
cord-104282-90t1m430	304	16	the	the	DT
cord-104282-90t1m430	304	17	tight	tight	JJ
cord-104282-90t1m430	304	18	anchoring	anchoring	NN
cord-104282-90t1m430	304	19	of	of	IN
cord-104282-90t1m430	304	20	the	the	DT
cord-104282-90t1m430	304	21	protein	protein	NN
cord-104282-90t1m430	304	22	in	in	IN
cord-104282-90t1m430	304	23	the	the	DT
cord-104282-90t1m430	304	24	ER	ER	NNP
cord-104282-90t1m430	304	25	membrane	membrane	NN
cord-104282-90t1m430	304	26	than	than	IN
cord-104282-90t1m430	304	27	a	a	DT
cord-104282-90t1m430	304	28	loop	loop	NN
cord-104282-90t1m430	304	29	model	model	NN
cord-104282-90t1m430	304	30	where	where	WRB
cord-104282-90t1m430	304	31	the	the	DT
cord-104282-90t1m430	304	32	hydrophilic	hydrophilic	JJ
cord-104282-90t1m430	304	33	tail	tail	NN
cord-104282-90t1m430	304	34	remains	remain	VBZ
cord-104282-90t1m430	304	35	on	on	IN
cord-104282-90t1m430	304	36	the	the	DT
cord-104282-90t1m430	304	37	cytoplasmic	cytoplasmic	JJ
cord-104282-90t1m430	304	38	surface	surface	NN
cord-104282-90t1m430	304	39	.	.	.
cord-104282-90t1m430	305	1	The	the	DT
cord-104282-90t1m430	305	2	Lys	Lys	NNP
cord-104282-90t1m430	305	3	-	-	HYPH
cord-104282-90t1m430	305	4	Asp	Asp	NNP
cord-104282-90t1m430	305	5	-	-	HYPH
cord-104282-90t1m430	305	6	Gln	Gln	NNP
cord-104282-90t1m430	305	7	-	-	HYPH
cord-104282-90t1m430	305	8	Leu	Leu	NNP
cord-104282-90t1m430	305	9	sequence	sequence	NN
cord-104282-90t1m430	305	10	of	of	IN
cord-104282-90t1m430	305	11	the	the	DT
cord-104282-90t1m430	305	12	tail	tail	NN
cord-104282-90t1m430	305	13	region	region	NN
cord-104282-90t1m430	305	14	strongly	strongly	RB
cord-104282-90t1m430	305	15	resembles	resemble	VBZ
cord-104282-90t1m430	305	16	the	the	DT
cord-104282-90t1m430	305	17	well	well	RB
cord-104282-90t1m430	305	18	-	-	HYPH
cord-104282-90t1m430	305	19	characterized	characterize	VBN
cord-104282-90t1m430	305	20	Lys	Lys	NNP
cord-104282-90t1m430	305	21	-	-	HYPH
cord-104282-90t1m430	305	22	Asp	Asp	NNP
cord-104282-90t1m430	305	23	-	-	HYPH
cord-104282-90t1m430	305	24	Glu	Glu	NNP
cord-104282-90t1m430	305	25	-	-	HYPH
cord-104282-90t1m430	305	26	Leu	Leu	NNP
cord-104282-90t1m430	305	27	retention	retention	NN
cord-104282-90t1m430	305	28	signal	signal	NN
cord-104282-90t1m430	305	29	found	find	VBN
cord-104282-90t1m430	305	30	in	in	IN
cord-104282-90t1m430	305	31	the	the	DT
cord-104282-90t1m430	305	32	ER	ER	NNP
cord-104282-90t1m430	305	33	luminal	luminal	JJ
cord-104282-90t1m430	305	34	proteins	protein	NNS
cord-104282-90t1m430	305	35	(	(	-LRB-
cord-104282-90t1m430	305	36	25	25	CD
cord-104282-90t1m430	305	37	)	)	-RRB-
cord-104282-90t1m430	305	38	,	,	,
cord-104282-90t1m430	305	39	suggesting	suggest	VBG
cord-104282-90t1m430	305	40	that	that	IN
cord-104282-90t1m430	305	41	the	the	DT
cord-104282-90t1m430	305	42	Lys	Lys	NNP
cord-104282-90t1m430	305	43	-	-	HYPH
cord-104282-90t1m430	305	44	Asp	Asp	NNP
cord-104282-90t1m430	305	45	-	-	HYPH
cord-104282-90t1m430	305	46	Gln	Gln	NNP
cord-104282-90t1m430	305	47	-	-	HYPH
cord-104282-90t1m430	305	48	Leu	Leu	NNP
cord-104282-90t1m430	305	49	sequence	sequence	NN
cord-104282-90t1m430	305	50	,	,	,
cord-104282-90t1m430	305	51	if	if	IN
cord-104282-90t1m430	305	52	translocated	translocate	VBN
cord-104282-90t1m430	305	53	into	into	IN
cord-104282-90t1m430	305	54	the	the	DT
cord-104282-90t1m430	305	55	lumen	lumen	NN
cord-104282-90t1m430	305	56	of	of	IN
cord-104282-90t1m430	305	57	the	the	DT
cord-104282-90t1m430	305	58	ER	ER	NNP
cord-104282-90t1m430	305	59	,	,	,
cord-104282-90t1m430	305	60	serves	serve	VBZ
cord-104282-90t1m430	305	61	as	as	IN
cord-104282-90t1m430	305	62	an	an	DT
cord-104282-90t1m430	305	63	ER	ER	NNP
cord-104282-90t1m430	305	64	retention	retention	NN
cord-104282-90t1m430	305	65	signal	signal	NN
cord-104282-90t1m430	305	66	of	of	IN
cord-104282-90t1m430	305	67	msALDH	msALDH	NNP
cord-104282-90t1m430	305	68	.	.	.
cord-104282-90t1m430	306	1	However	however	RB
cord-104282-90t1m430	306	2	,	,	,
cord-104282-90t1m430	306	3	Andres	Andres	NNP
cord-104282-90t1m430	306	4	et	et	NNP
cord-104282-90t1m430	306	5	al	al	NNP
cord-104282-90t1m430	306	6	.	.	NNP
cord-104282-90t1m430	306	7	have	have	VBP
cord-104282-90t1m430	306	8	shown	show	VBN
cord-104282-90t1m430	306	9	that	that	WDT
cord-104282-90t1m430	306	10	proneuropeptide	proneuropeptide	NNP
cord-104282-90t1m430	306	11	Y	Y	NNP
cord-104282-90t1m430	306	12	mutant	mutant	NN
cord-104282-90t1m430	306	13	bearing	bear	VBG
cord-104282-90t1m430	306	14	the	the	DT
cord-104282-90t1m430	306	15	Lys	Lys	NNP
cord-104282-90t1m430	306	16	-	-	HYPH
cord-104282-90t1m430	306	17	Asp	Asp	NNP
cord-104282-90t1m430	306	18	-	-	HYPH
cord-104282-90t1m430	306	19	Gln	Gln	NNP
cord-104282-90t1m430	306	20	-	-	HYPH
cord-104282-90t1m430	306	21	Leu	Leu	NNP
cord-104282-90t1m430	306	22	sequence	sequence	NN
cord-104282-90t1m430	306	23	at	at	IN
cord-104282-90t1m430	306	24	the	the	DT
cord-104282-90t1m430	306	25	carboxyl	carboxyl	NN
cord-104282-90t1m430	306	26	terminus	terminus	NN
cord-104282-90t1m430	306	27	is	be	VBZ
cord-104282-90t1m430	306	28	processed	process	VBN
cord-104282-90t1m430	306	29	and	and	CC
cord-104282-90t1m430	306	30	secreted	secrete	VBN
cord-104282-90t1m430	306	31	like	like	IN
cord-104282-90t1m430	306	32	wild	wild	JJ
cord-104282-90t1m430	306	33	-	-	HYPH
cord-104282-90t1m430	306	34	type	type	NN
cord-104282-90t1m430	306	35	proneuropeptide	proneuropeptide	IN
cord-104282-90t1m430	306	36	Y	Y	NNP
cord-104282-90t1m430	306	37	when	when	WRB
cord-104282-90t1m430	306	38	expressed	express	VBN
cord-104282-90t1m430	306	39	in	in	IN
cord-104282-90t1m430	306	40	AtT-20	AtT-20	NNP
cord-104282-90t1m430	306	41	cells	cell	NNS
cord-104282-90t1m430	306	42	,	,	,
cord-104282-90t1m430	306	43	despite	despite	IN
cord-104282-90t1m430	306	44	the	the	DT
cord-104282-90t1m430	306	45	intraceUular	intraceuular	JJ
cord-104282-90t1m430	306	46	retention	retention	NN
cord-104282-90t1m430	306	47	of	of	IN
cord-104282-90t1m430	306	48	the	the	DT
cord-104282-90t1m430	306	49	unprocessed	unprocessed	JJ
cord-104282-90t1m430	306	50	proneuropep-	proneuropep-	NN
cord-104282-90t1m430	306	51	Nuclear	Nuclear	NNP
cord-104282-90t1m430	306	52	protein	protein	NN
cord-104282-90t1m430	306	53	import	import	NN
cord-104282-90t1m430	306	54	in	in	IN
cord-104282-90t1m430	306	55	permeabilized	permeabilized	JJ
cord-104282-90t1m430	306	56	mammalian	mammalian	JJ
cord-104282-90t1m430	306	57	cells	cell	NNS
cord-104282-90t1m430	306	58	requires	require	VBZ
cord-104282-90t1m430	306	59	soluble	soluble	JJ
cord-104282-90t1m430	306	60	cytoplasmic	cytoplasmic	JJ
cord-104282-90t1m430	306	61	factors	factor	NNS
cord-104282-90t1m430	306	62	Distribution	distribution	NN
cord-104282-90t1m430	306	63	of	of	IN
cord-104282-90t1m430	306	64	protein	protein	NN
cord-104282-90t1m430	306	65	disulfide	disulfide	NN
cord-104282-90t1m430	306	66	isomerase	isomerase	NN
cord-104282-90t1m430	306	67	in	in	IN
cord-104282-90t1m430	306	68	rat	rat	NN
cord-104282-90t1m430	306	69	hepatocytes	hepatocyte	NNS
cord-104282-90t1m430	306	70	Mechanisms	Mechanisms	NNPS
cord-104282-90t1m430	306	71	of	of	IN
cord-104282-90t1m430	306	72	integration	integration	NN
cord-104282-90t1m430	306	73	of	of	IN
cord-104282-90t1m430	306	74	de	de	NNP
cord-104282-90t1m430	306	75	novo	novo	NNP
cord-104282-90t1m430	306	76	-	-	HYPH
cord-104282-90t1m430	306	77	synthesized	synthesize	VBN
cord-104282-90t1m430	306	78	polypeptides	polypeptide	NNS
cord-104282-90t1m430	306	79	into	into	IN
cord-104282-90t1m430	306	80	membranes	membrane	NNS
cord-104282-90t1m430	306	81	:	:	:
cord-104282-90t1m430	306	82	signalrecognition	signalrecognition	NN
cord-104282-90t1m430	306	83	particle	particle	NN
cord-104282-90t1m430	306	84	is	be	VBZ
cord-104282-90t1m430	306	85	required	require	VBN
cord-104282-90t1m430	306	86	for	for	IN
cord-104282-90t1m430	306	87	integration	integration	NN
cord-104282-90t1m430	306	88	into	into	IN
cord-104282-90t1m430	306	89	microsomal	microsomal	JJ
cord-104282-90t1m430	306	90	membranes	membrane	NNS
cord-104282-90t1m430	306	91	of	of	IN
cord-104282-90t1m430	306	92	calcium	calcium	NN
cord-104282-90t1m430	306	93	ATPase	atpase	NN
cord-104282-90t1m430	306	94	and	and	CC
cord-104282-90t1m430	306	95	of	of	IN
cord-104282-90t1m430	306	96	lens	lens	NN
cord-104282-90t1m430	307	1	MP26	mp26	NN
cord-104282-90t1m430	307	2	but	but	CC
cord-104282-90t1m430	307	3	not	not	RB
cord-104282-90t1m430	307	4	of	of	IN
cord-104282-90t1m430	307	5	cytochrome	cytochrome	NN
cord-104282-90t1m430	307	6	b5	b5	NNP
cord-104282-90t1m430	307	7	Variants	Variants	NNPS
cord-104282-90t1m430	307	8	of	of	IN
cord-104282-90t1m430	307	9	the	the	DT
cord-104282-90t1m430	307	10	carboxylterminal	carboxylterminal	JJ
cord-104282-90t1m430	307	11	KDEL	KDEL	NNP
cord-104282-90t1m430	307	12	sequence	sequence	NN
cord-104282-90t1m430	307	13	direct	direct	JJ
cord-104282-90t1m430	307	14	intracellular	intracellular	JJ
cord-104282-90t1m430	307	15	retention	retention	NN
cord-104282-90t1m430	308	1	Intracellular	Intracellular	NNP
cord-104282-90t1m430	308	2	protein	protein	NN
cord-104282-90t1m430	308	3	topogenesis	topogenesis	NN
cord-104282-90t1m430	308	4	Phase	phase	NN
cord-104282-90t1m430	308	5	separation	separation	NN
cord-104282-90t1m430	308	6	of	of	IN
cord-104282-90t1m430	308	7	integral	integral	JJ
cord-104282-90t1m430	308	8	membrane	membrane	NN
cord-104282-90t1m430	308	9	proteins	protein	NNS
cord-104282-90t1m430	308	10	in	in	IN
cord-104282-90t1m430	308	11	Triton	Triton	NNP
cord-104282-90t1m430	308	12	X-114	X-114	VBZ
cord-104282-90t1m430	308	13	solution	solution	NN
cord-104282-90t1m430	309	1	A	a	DT
cord-104282-90t1m430	309	2	rapid	rapid	JJ
cord-104282-90t1m430	309	3	and	and	CC
cord-104282-90t1m430	309	4	sensitive	sensitive	JJ
cord-104282-90t1m430	309	5	method	method	NN
cord-104282-90t1m430	309	6	for	for	IN
cord-104282-90t1m430	309	7	the	the	DT
cord-104282-90t1m430	309	8	quantitation	quantitation	NN
cord-104282-90t1m430	309	9	of	of	IN
cord-104282-90t1m430	309	10	microgram	microgram	NN
cord-104282-90t1m430	309	11	quantities	quantity	NNS
cord-104282-90t1m430	309	12	of	of	IN
cord-104282-90t1m430	309	13	protein	protein	NN
cord-104282-90t1m430	309	14	utilizing	utilize	VBG
cord-104282-90t1m430	309	15	the	the	DT
cord-104282-90t1m430	309	16	principle	principle	NN
cord-104282-90t1m430	309	17	of	of	IN
cord-104282-90t1m430	309	18	protein	protein	NN
cord-104282-90t1m430	309	19	-	-	HYPH
cord-104282-90t1m430	309	20	dye	dye	NN
cord-104282-90t1m430	309	21	binding	bind	VBG
cord-104282-90t1m430	309	22	Western	western	JJ
cord-104282-90t1m430	309	23	blotting	blotting	NN
cord-104282-90t1m430	309	24	"	"	''
cord-104282-90t1m430	309	25	:	:	:
cord-104282-90t1m430	309	26	electrophoretic	electrophoretic	JJ
cord-104282-90t1m430	309	27	transfer	transfer	NN
cord-104282-90t1m430	309	28	of	of	IN
cord-104282-90t1m430	309	29	proteins	protein	NNS
cord-104282-90t1m430	309	30	from	from	IN
cord-104282-90t1m430	309	31	sodium	sodium	NN
cord-104282-90t1m430	309	32	dodecyl	dodecyl	NNP
cord-104282-90t1m430	309	33	sulfate	sulfate	NN
cord-104282-90t1m430	309	34	-	-	HYPH
cord-104282-90t1m430	309	35	polyacrylamide	polyacrylamide	NN
cord-104282-90t1m430	309	36	gels	gel	NNS
cord-104282-90t1m430	309	37	to	to	IN
cord-104282-90t1m430	309	38	unmodified	unmodified	JJ
cord-104282-90t1m430	309	39	nitrocellulose	nitrocellulose	NN
cord-104282-90t1m430	309	40	and	and	CC
cord-104282-90t1m430	309	41	radiographic	radiographic	JJ
cord-104282-90t1m430	309	42	detection	detection	NN
cord-104282-90t1m430	309	43	with	with	IN
cord-104282-90t1m430	309	44	antibody	antibody	NN
cord-104282-90t1m430	309	45	and	and	CC
cord-104282-90t1m430	309	46	radioiodinated	radioiodinated	JJ
cord-104282-90t1m430	309	47	protein	protein	NN
cord-104282-90t1m430	309	48	A	a	DT
cord-104282-90t1m430	309	49	Cloning	cloning	NN
cord-104282-90t1m430	309	50	of	of	IN
cord-104282-90t1m430	309	51	a	a	DT
cord-104282-90t1m430	309	52	cDNA	cdna	NN
cord-104282-90t1m430	309	53	for	for	IN
cord-104282-90t1m430	309	54	a	a	DT
cord-104282-90t1m430	309	55	major	major	JJ
cord-104282-90t1m430	309	56	human	human	JJ
cord-104282-90t1m430	309	57	protein	protein	NN
cord-104282-90t1m430	309	58	-	-	HYPH
cord-104282-90t1m430	309	59	tyrosinephosphatase	tyrosinephosphatase	NN
cord-104282-90t1m430	310	1	The	the	DT
cord-104282-90t1m430	310	2	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	310	3	amino	amino	NN
cord-104282-90t1m430	310	4	-	-	HYPH
cord-104282-90t1m430	310	5	terminal	terminal	NN
cord-104282-90t1m430	310	6	sequence	sequence	NN
cord-104282-90t1m430	310	7	of	of	IN
cord-104282-90t1m430	310	8	bovine	bovine	JJ
cord-104282-90t1m430	310	9	17~-hydroxylase	17~-hydroxylase	CD
cord-104282-90t1m430	310	10	is	be	VBZ
cord-104282-90t1m430	310	11	required	require	VBN
cord-104282-90t1m430	310	12	for	for	IN
cord-104282-90t1m430	310	13	the	the	DT
cord-104282-90t1m430	310	14	expression	expression	NN
cord-104282-90t1m430	310	15	of	of	IN
cord-104282-90t1m430	310	16	a	a	DT
cord-104282-90t1m430	310	17	functional	functional	JJ
cord-104282-90t1m430	310	18	hemoprotein	hemoprotein	NN
cord-104282-90t1m430	310	19	in	in	IN
cord-104282-90t1m430	310	20	COS	COS	NNP
cord-104282-90t1m430	310	21	-	-	HYPH
cord-104282-90t1m430	310	22	I	-PRON-	PRP
cord-104282-90t1m430	310	23	cells	cell	NNS
cord-104282-90t1m430	311	1	The	the	DT
cord-104282-90t1m430	311	2	specific	specific	JJ
cord-104282-90t1m430	311	3	subcellular	subcellular	JJ
cord-104282-90t1m430	311	4	localization	localization	NN
cord-104282-90t1m430	311	5	of	of	IN
cord-104282-90t1m430	311	6	two	two	CD
cord-104282-90t1m430	311	7	isoforms	isoform	NNS
cord-104282-90t1m430	311	8	of	of	IN
cord-104282-90t1m430	311	9	cytochrome	cytochrome	NN
cord-104282-90t1m430	311	10	b5	b5	NNP
cord-104282-90t1m430	311	11	suggests	suggest	VBZ
cord-104282-90t1m430	311	12	novel	novel	NN
cord-104282-90t1m430	311	13	targeting	target	VBG
cord-104282-90t1m430	311	14	pathways	pathway	NNS
cord-104282-90t1m430	312	1	The	the	DT
cord-104282-90t1m430	312	2	nontransmembrane	nontransmembrane	NNP
cord-104282-90t1m430	312	3	tyrosine	tyrosine	NN
cord-104282-90t1m430	312	4	phosphatase	phosphatase	NN
cord-104282-90t1m430	312	5	PTP	PTP	NNP
cord-104282-90t1m430	312	6	1B	1b	NN
cord-104282-90t1m430	312	7	localizes	localize	VBZ
cord-104282-90t1m430	312	8	to	to	IN
cord-104282-90t1m430	312	9	the	the	DT
cord-104282-90t1m430	312	10	endoplasmic	endoplasmic	NN
cord-104282-90t1m430	312	11	reticulum	reticulum	NN
cord-104282-90t1m430	312	12	via	via	IN
cord-104282-90t1m430	312	13	its	-PRON-	PRP$
cord-104282-90t1m430	312	14	35	35	CD
cord-104282-90t1m430	312	15	amino	amino	NN
cord-104282-90t1m430	312	16	acid	acid	NN
cord-104282-90t1m430	312	17	C	c	NN
cord-104282-90t1m430	312	18	-	-	HYPH
cord-104282-90t1m430	312	19	terminal	terminal	NN
cord-104282-90t1m430	312	20	sequence	sequence	NN
cord-104282-90t1m430	312	21	Isolation	isolation	NN
cord-104282-90t1m430	312	22	of	of	IN
cord-104282-90t1m430	312	23	intracellular	intracellular	JJ
cord-104282-90t1m430	312	24	membranes	membrane	NNS
cord-104282-90t1m430	312	25	by	by	IN
cord-104282-90t1m430	312	26	means	mean	NNS
cord-104282-90t1m430	312	27	of	of	IN
cord-104282-90t1m430	312	28	sodium	sodium	NN
cord-104282-90t1m430	312	29	carbonate	carbonate	NN
cord-104282-90t1m430	312	30	treatment	treatment	NN
cord-104282-90t1m430	312	31	:	:	:
cord-104282-90t1m430	312	32	application	application	NN
cord-104282-90t1m430	312	33	to	to	IN
cord-104282-90t1m430	312	34	endoplasmic	endoplasmic	NN
cord-104282-90t1m430	312	35	reticulum	reticulum	NN
cord-104282-90t1m430	313	1	A	a	DT
cord-104282-90t1m430	313	2	conserved	conserved	JJ
cord-104282-90t1m430	313	3	tripeptide	tripeptide	NN
cord-104282-90t1m430	313	4	sorts	sort	NNS
cord-104282-90t1m430	313	5	proteins	protein	NNS
cord-104282-90t1m430	313	6	to	to	IN
cord-104282-90t1m430	313	7	peroxisomes	peroxisome	NNS
cord-104282-90t1m430	313	8	A	a	DT
cord-104282-90t1m430	313	9	mitochondrial	mitochondrial	JJ
cord-104282-90t1m430	313	10	import	import	NN
cord-104282-90t1m430	313	11	factor	factor	NN
cord-104282-90t1m430	313	12	purified	purify	VBN
cord-104282-90t1m430	313	13	from	from	IN
cord-104282-90t1m430	313	14	rat	rat	NN
cord-104282-90t1m430	313	15	liver	liver	NN
cord-104282-90t1m430	313	16	cytosol	cytosol	NN
cord-104282-90t1m430	313	17	is	be	VBZ
cord-104282-90t1m430	313	18	an	an	DT
cord-104282-90t1m430	313	19	ATF	atf	NN
cord-104282-90t1m430	313	20	-	-	HYPH
cord-104282-90t1m430	313	21	dependent	dependent	JJ
cord-104282-90t1m430	313	22	conformational	conformational	JJ
cord-104282-90t1m430	313	23	modulator	modulator	NN
cord-104282-90t1m430	313	24	for	for	IN
cord-104282-90t1m430	313	25	precursor	precursor	NN
cord-104282-90t1m430	313	26	proteins	protein	NNS
cord-104282-90t1m430	313	27	Sequence	sequence	NN
cord-104282-90t1m430	313	28	requirements	requirement	NNS
cord-104282-90t1m430	313	29	for	for	IN
cord-104282-90t1m430	313	30	nuclear	nuclear	JJ
cord-104282-90t1m430	313	31	location	location	NN
cord-104282-90t1m430	313	32	of	of	IN
cord-104282-90t1m430	313	33	simian	simian	JJ
cord-104282-90t1m430	313	34	virus	virus	NN
cord-104282-90t1m430	313	35	40	40	CD
cord-104282-90t1m430	313	36	large	large	JJ
cord-104282-90t1m430	313	37	T	t	NN
cord-104282-90t1m430	313	38	antigen	antigen	NN
cord-104282-90t1m430	313	39	Preparations	preparation	NNS
cord-104282-90t1m430	313	40	of	of	IN
cord-104282-90t1m430	313	41	succinate	succinate	NN
cord-104282-90t1m430	313	42	-	-	HYPH
cord-104282-90t1m430	313	43	cytochrome	cytochrome	JJ
cord-104282-90t1m430	313	44	c	c	NN
cord-104282-90t1m430	313	45	reductase	reductase	NN
cord-104282-90t1m430	313	46	and	and	CC
cord-104282-90t1m430	313	47	the	the	DT
cord-104282-90t1m430	313	48	cytochrome	cytochrome	NN
cord-104282-90t1m430	313	49	b	b	NN
cord-104282-90t1m430	313	50	-	-	HYPH
cord-104282-90t1m430	313	51	c1	c1	NN
cord-104282-90t1m430	313	52	particle	particle	NN
cord-104282-90t1m430	313	53	,	,	,
cord-104282-90t1m430	313	54	and	and	CC
cord-104282-90t1m430	313	55	reconstitution	reconstitution	NN
cord-104282-90t1m430	313	56	of	of	IN
cord-104282-90t1m430	313	57	succinate	succinate	NN
cord-104282-90t1m430	313	58	-	-	HYPH
cord-104282-90t1m430	313	59	cytochrome	cytochrome	JJ
cord-104282-90t1m430	313	60	c	c	NN
cord-104282-90t1m430	313	61	reductase	reductase	NN
cord-104282-90t1m430	314	1	The	the	DT
cord-104282-90t1m430	314	2	biogenesis	biogenesis	NN
cord-104282-90t1m430	314	3	of	of	IN
cord-104282-90t1m430	314	4	lysosomes	lysosome	VBN
cord-104282-90t1m430	314	5	Oligonucleotide	Oligonucleotide	NNP
cord-104282-90t1m430	314	6	-	-	HYPH
cord-104282-90t1m430	314	7	directed	direct	VBN
cord-104282-90t1m430	314	8	construction	construction	NN
cord-104282-90t1m430	314	9	of	of	IN
cord-104282-90t1m430	314	10	mutations	mutation	NNS
cord-104282-90t1m430	314	11	via	via	IN
cord-104282-90t1m430	314	12	gapped	gap	VBN
cord-104282-90t1m430	314	13	duplex	duplex	NN
cord-104282-90t1m430	314	14	DNA	dna	NN
cord-104282-90t1m430	314	15	Cleavage	Cleavage	NNP
cord-104282-90t1m430	314	16	of	of	IN
cord-104282-90t1m430	314	17	structural	structural	JJ
cord-104282-90t1m430	314	18	proteins	protein	NNS
cord-104282-90t1m430	314	19	during	during	IN
cord-104282-90t1m430	314	20	the	the	DT
cord-104282-90t1m430	314	21	assembly	assembly	NN
cord-104282-90t1m430	314	22	of	of	IN
cord-104282-90t1m430	314	23	the	the	DT
cord-104282-90t1m430	314	24	head	head	NN
cord-104282-90t1m430	314	25	of	of	IN
cord-104282-90t1m430	314	26	bacteriophage	bacteriophage	NN
cord-104282-90t1m430	314	27	T4	T4	NNP
cord-104282-90t1m430	314	28	Cylochrome	Cylochrome	NNP
cord-104282-90t1m430	315	1	P-450	P-450	NNP
cord-104282-90t1m430	315	2	and	and	CC
cord-104282-90t1m430	315	3	NADPH	nadph	NN
cord-104282-90t1m430	315	4	-	-	HYPH
cord-104282-90t1m430	315	5	cytochrome	cytochrome	NN
cord-104282-90t1m430	315	6	P-450	p-450	NN
cord-104282-90t1m430	315	7	reductase	reductase	NN
cord-104282-90t1m430	315	8	are	be	VBP
cord-104282-90t1m430	315	9	degraded	degrade	VBN
cord-104282-90t1m430	315	10	in	in	IN
cord-104282-90t1m430	315	11	the	the	DT
cord-104282-90t1m430	315	12	autolysosomes	autolysosome	NNS
cord-104282-90t1m430	315	13	in	in	IN
cord-104282-90t1m430	315	14	rat	rat	NN
cord-104282-90t1m430	315	15	liver	liver	NN
cord-104282-90t1m430	315	16	Immunoelectron	Immunoelectron	NNP
cord-104282-90t1m430	315	17	microscope	microscope	NN
cord-104282-90t1m430	315	18	localization	localization	NN
cord-104282-90t1m430	315	19	of	of	IN
cord-104282-90t1m430	315	20	cytochrome	cytochrome	NN
cord-104282-90t1m430	315	21	P-450	p-450	NN
cord-104282-90t1m430	316	1	reductase	reductase	NNP
cord-104282-90t1m430	316	2	on	on	IN
cord-104282-90t1m430	316	3	microsomes	microsome	NNS
cord-104282-90t1m430	316	4	and	and	CC
cord-104282-90t1m430	316	5	other	other	JJ
cord-104282-90t1m430	316	6	membrane	membrane	NN
cord-104282-90t1m430	316	7	structures	structure	NNS
cord-104282-90t1m430	316	8	of	of	IN
cord-104282-90t1m430	316	9	rat	rat	NN
cord-104282-90t1m430	316	10	hepatocytes	hepatocyte	NNS
cord-104282-90t1m430	317	1	The	the	DT
cord-104282-90t1m430	317	2	carboxy	carboxy	JJ
cord-104282-90t1m430	317	3	-	-	HYPH
cord-104282-90t1m430	317	4	terminal	terminal	NN
cord-104282-90t1m430	317	5	10	10	CD
cord-104282-90t1m430	317	6	amino	amino	NN
cord-104282-90t1m430	317	7	acid	acid	NN
cord-104282-90t1m430	317	8	residues	residue	NNS
cord-104282-90t1m430	317	9	of	of	IN
cord-104282-90t1m430	317	10	cytochrome	cytochrome	NN
cord-104282-90t1m430	317	11	b5	b5	NN
cord-104282-90t1m430	317	12	are	be	VBP
cord-104282-90t1m430	317	13	necessary	necessary	JJ
cord-104282-90t1m430	317	14	for	for	IN
cord-104282-90t1m430	317	15	its	-PRON-	PRP$
cord-104282-90t1m430	317	16	targeting	targeting	NN
cord-104282-90t1m430	317	17	to	to	IN
cord-104282-90t1m430	317	18	the	the	DT
cord-104282-90t1m430	317	19	endoplasmic	endoplasmic	NN
cord-104282-90t1m430	317	20	reticulure	reticulure	NN
cord-104282-90t1m430	317	21	Molecular	molecular	JJ
cord-104282-90t1m430	317	22	cloning	cloning	NN
cord-104282-90t1m430	317	23	,	,	,
cord-104282-90t1m430	317	24	sequencing	sequencing	NN
cord-104282-90t1m430	317	25	,	,	,
cord-104282-90t1m430	317	26	and	and	CC
cord-104282-90t1m430	317	27	expression	expression	NN
cord-104282-90t1m430	317	28	of	of	IN
cord-104282-90t1m430	317	29	cDNA	cdna	NN
cord-104282-90t1m430	317	30	for	for	IN
cord-104282-90t1m430	317	31	rat	rat	NNP
cord-104282-90t1m430	317	32	liver	liver	NNP
cord-104282-90t1m430	317	33	microsomul	microsomul	NNS
cord-104282-90t1m430	318	1	aldehyde	aldehyde	NN
cord-104282-90t1m430	318	2	dehydrogenase	dehydrogenase	NN
cord-104282-90t1m430	318	3	A	a	DT
cord-104282-90t1m430	318	4	C	c	NN
cord-104282-90t1m430	318	5	-	-	HYPH
cord-104282-90t1m430	318	6	terminal	terminal	NN
cord-104282-90t1m430	318	7	signal	signal	NN
cord-104282-90t1m430	318	8	prevents	prevent	VBZ
cord-104282-90t1m430	318	9	secretion	secretion	NN
cord-104282-90t1m430	318	10	of	of	IN
cord-104282-90t1m430	318	11	luminal	luminal	JJ
cord-104282-90t1m430	318	12	ER	er	NN
cord-104282-90t1m430	318	13	proteins	protein	NNS
cord-104282-90t1m430	318	14	Sequences	Sequences	NNPS
cord-104282-90t1m430	318	15	within	within	IN
cord-104282-90t1m430	318	16	and	and	CC
cord-104282-90t1m430	318	17	adjacent	adjacent	JJ
cord-104282-90t1m430	318	18	to	to	IN
cord-104282-90t1m430	318	19	the	the	DT
cord-104282-90t1m430	318	20	transmembrane	transmembrane	NNP
cord-104282-90t1m430	318	21	segment	segment	NN
cord-104282-90t1m430	318	22	of	of	IN
cord-104282-90t1m430	318	23	c~-2,6-sialyltransferase	c~-2,6-sialyltransferase	NNS
cord-104282-90t1m430	318	24	specify	specify	VBP
cord-104282-90t1m430	318	25	Golgi	Golgi	NNP
cord-104282-90t1m430	318	26	retention	retention	NN
cord-104282-90t1m430	318	27	Short	short	JJ
cord-104282-90t1m430	318	28	cytoplasmic	cytoplasmic	JJ
cord-104282-90t1m430	318	29	sequences	sequence	NNS
cord-104282-90t1m430	318	30	serve	serve	VBP
cord-104282-90t1m430	318	31	as	as	IN
cord-104282-90t1m430	318	32	retention	retention	NN
cord-104282-90t1m430	318	33	signals	signal	NNS
cord-104282-90t1m430	318	34	for	for	IN
cord-104282-90t1m430	318	35	transmembrane	transmembrane	NN
cord-104282-90t1m430	318	36	proteins	protein	NNS
cord-104282-90t1m430	318	37	in	in	IN
cord-104282-90t1m430	318	38	the	the	DT
cord-104282-90t1m430	318	39	endoplasmic	endoplasmic	NN
cord-104282-90t1m430	318	40	reticulum	reticulum	NN
cord-104282-90t1m430	318	41	Ribosome	Ribosome	NNP
cord-104282-90t1m430	318	42	-	-	HYPH
cord-104282-90t1m430	318	43	binding	bind	VBG
cord-104282-90t1m430	318	44	protein	protein	NN
cord-104282-90t1m430	318	45	p34	p34	CD
cord-104282-90t1m430	318	46	is	be	VBZ
cord-104282-90t1m430	318	47	a	a	DT
cord-104282-90t1m430	318	48	member	member	NN
cord-104282-90t1m430	318	49	of	of	IN
cord-104282-90t1m430	318	50	the	the	DT
cord-104282-90t1m430	318	51	leucine	leucine	NN
cord-104282-90t1m430	318	52	-	-	HYPH
cord-104282-90t1m430	318	53	richrepeat	richrepeat	NN
cord-104282-90t1m430	318	54	-	-	HYPH
cord-104282-90t1m430	318	55	protein	protein	NN
cord-104282-90t1m430	318	56	superfamily	superfamily	NN
cord-104282-90t1m430	318	57	Studies	Studies	NNPS
cord-104282-90t1m430	318	58	on	on	IN
cord-104282-90t1m430	318	59	the	the	DT
cord-104282-90t1m430	318	60	biosynthesis	biosynthesis	NN
cord-104282-90t1m430	318	61	of	of	IN
cord-104282-90t1m430	318	62	microsomal	microsomal	NNP
cord-104282-90t1m430	318	63	proteins	protein	NNS
cord-104282-90t1m430	318	64	:	:	:
cord-104282-90t1m430	318	65	site	site	NN
cord-104282-90t1m430	318	66	of	of	IN
cord-104282-90t1m430	318	67	synthesis	synthesis	NN
cord-104282-90t1m430	318	68	and	and	CC
cord-104282-90t1m430	318	69	mode	mode	NN
cord-104282-90t1m430	318	70	of	of	IN
cord-104282-90t1m430	318	71	insertion	insertion	NN
cord-104282-90t1m430	318	72	of	of	IN
cord-104282-90t1m430	318	73	cytochrome	cytochrome	NN
cord-104282-90t1m430	318	74	bS	bS	NNP
cord-104282-90t1m430	318	75	,	,	,
cord-104282-90t1m430	318	76	cytochrome	cytochrome	NN
cord-104282-90t1m430	318	77	b5	b5	NNP
cord-104282-90t1m430	318	78	reductas¢	reductas¢	NNP
cord-104282-90t1m430	318	79	,	,	,
cord-104282-90t1m430	318	80	cytochrome	cytochrome	NN
cord-104282-90t1m430	318	81	P-450	p-450	XX
cord-104282-90t1m430	319	1	reductase	reductase	NN
cord-104282-90t1m430	319	2	and	and	CC
cord-104282-90t1m430	319	3	epoxide	epoxide	NN
cord-104282-90t1m430	319	4	hydrolase	hydrolase	NN
cord-104282-90t1m430	319	5	.	.	.
cord-104282-90t1m430	320	1	Fur	Fur	NNP
cord-104282-90t1m430	320	2	A	a	DT
cord-104282-90t1m430	320	3	cDNA	cdna	NN
cord-104282-90t1m430	320	4	cloning	cloning	NN
cord-104282-90t1m430	320	5	vector	vector	NN
cord-104282-90t1m430	320	6	that	that	WDT
cord-104282-90t1m430	320	7	permits	permit	VBZ
cord-104282-90t1m430	320	8	expression	expression	NN
cord-104282-90t1m430	320	9	of	of	IN
cord-104282-90t1m430	320	10	cDNA	cdna	NN
cord-104282-90t1m430	320	11	inserts	insert	NNS
cord-104282-90t1m430	320	12	in	in	IN
cord-104282-90t1m430	320	13	mammalian	mammalian	JJ
cord-104282-90t1m430	320	14	cells	cell	NNS
cord-104282-90t1m430	321	1	A	a	DT
cord-104282-90t1m430	321	2	new	new	JJ
cord-104282-90t1m430	321	3	method	method	NN
cord-104282-90t1m430	321	4	for	for	IN
cord-104282-90t1m430	321	5	simultaneous	simultaneous	JJ
cord-104282-90t1m430	321	6	purification	purification	NN
cord-104282-90t1m430	321	7	of	of	IN
cord-104282-90t1m430	321	8	cytochrnme	cytochrnme	NNP
cord-104282-90t1m430	321	9	b5	b5	NNP
cord-104282-90t1m430	321	10	and	and	CC
cord-104282-90t1m430	321	11	NADPH	NADPH	NNP
cord-104282-90t1m430	321	12	-	-	HYPH
cord-104282-90t1m430	321	13	cytochrome	cytochrome	NN
cord-104282-90t1m430	321	14	c	c	NN
cord-104282-90t1m430	321	15	reductase	reductase	NN
cord-104282-90t1m430	321	16	from	from	IN
cord-104282-90t1m430	321	17	rat	rat	NN
cord-104282-90t1m430	321	18	liver	liver	NN
cord-104282-90t1m430	321	19	microsomes	microsomes	NNP
cord-104282-90t1m430	321	20	Chemical	Chemical	NNP
cord-104282-90t1m430	321	21	structure	structure	NN
cord-104282-90t1m430	321	22	of	of	IN
cord-104282-90t1m430	321	23	rat	rat	NN
cord-104282-90t1m430	321	24	cytochrome	cytochrome	NN
cord-104282-90t1m430	321	25	bS	bS	NNP
cord-104282-90t1m430	321	26	:	:	:
cord-104282-90t1m430	321	27	isolation	isolation	NN
cord-104282-90t1m430	321	28	of	of	IN
cord-104282-90t1m430	321	29	peptides	peptide	NNS
cord-104282-90t1m430	321	30	by	by	IN
cord-104282-90t1m430	321	31	high	high	JJ
cord-104282-90t1m430	321	32	-	-	HYPH
cord-104282-90t1m430	321	33	pressure	pressure	NN
cord-104282-90t1m430	321	34	liquid	liquid	NN
cord-104282-90t1m430	321	35	chrom	chrom	NNP
cord-104282-90t1m430	321	36	~	~	NFP
cord-104282-90t1m430	321	37	atography	atography	NNP
cord-104282-90t1m430	321	38	Biosynthetic	biosynthetic	JJ
cord-104282-90t1m430	321	39	protein	protein	NN
cord-104282-90t1m430	321	40	transport	transport	NN
cord-104282-90t1m430	321	41	and	and	CC
cord-104282-90t1m430	321	42	sorting	sorting	NN
cord-104282-90t1m430	321	43	by	by	IN
cord-104282-90t1m430	321	44	the	the	DT
cord-104282-90t1m430	321	45	endoplasmic	endoplasmic	NN
cord-104282-90t1m430	321	46	reticulum	reticulum	NN
cord-104282-90t1m430	321	47	and	and	CC
cord-104282-90t1m430	321	48	Golgi	Golgi	NNP
cord-104282-90t1m430	321	49	Molecular	Molecular	NNP
cord-104282-90t1m430	321	50	cloning	cloning	NN
cord-104282-90t1m430	321	51	of	of	IN
cord-104282-90t1m430	321	52	the	the	DT
cord-104282-90t1m430	321	53	~-subunit	~-subunit	NNS
cord-104282-90t1m430	321	54	of	of	IN
cord-104282-90t1m430	321	55	human	human	JJ
cord-104282-90t1m430	321	56	prolyi	prolyi	NN
cord-104282-90t1m430	321	57	4-hydroxylase	4-hydroxylase	CD
cord-104282-90t1m430	321	58	.	.	.
cord-104282-90t1m430	322	1	This	this	DT
cord-104282-90t1m430	322	2	subunit	subunit	NN
cord-104282-90t1m430	322	3	and	and	CC
cord-104282-90t1m430	322	4	protein	protein	NN
cord-104282-90t1m430	322	5	disulfide	disulfide	NN
cord-104282-90t1m430	322	6	isomerase	isomerase	NN
cord-104282-90t1m430	322	7	are	be	VBP
cord-104282-90t1m430	322	8	products	product	NNS
cord-104282-90t1m430	322	9	of	of	IN
cord-104282-90t1m430	322	10	the	the	DT
cord-104282-90t1m430	322	11	same	same	JJ
cord-104282-90t1m430	322	12	gene	gene	NN
cord-104282-90t1m430	322	13	Protein	Protein	NNP
cord-104282-90t1m430	322	14	translocation	translocation	NN
cord-104282-90t1m430	322	15	across	across	IN
cord-104282-90t1m430	322	16	the	the	DT
cord-104282-90t1m430	322	17	ER	ER	NNP
cord-104282-90t1m430	322	18	requires	require	VBZ
cord-104282-90t1m430	322	19	a	a	DT
cord-104282-90t1m430	322	20	functional	functional	JJ
cord-104282-90t1m430	322	21	GTP	gtp	NN
cord-104282-90t1m430	322	22	binding	bind	VBG
cord-104282-90t1m430	322	23	site	site	NN
cord-104282-90t1m430	322	24	in	in	IN
cord-104282-90t1m430	322	25	the	the	DT
cord-104282-90t1m430	322	26	a	a	DT
cord-104282-90t1m430	322	27	subunit	subunit	NN
cord-104282-90t1m430	322	28	of	of	IN
cord-104282-90t1m430	322	29	the	the	DT
cord-104282-90t1m430	322	30	signal	signal	NN
cord-104282-90t1m430	322	31	recognition	recognition	NN
cord-104282-90t1m430	322	32	particle	particle	NN
cord-104282-90t1m430	322	33	receptor	receptor	NN
cord-104282-90t1m430	322	34	Transport	Transport	NNP
cord-104282-90t1m430	322	35	of	of	IN
cord-104282-90t1m430	322	36	proteins	protein	NNS
cord-104282-90t1m430	322	37	across	across	IN
cord-104282-90t1m430	322	38	the	the	DT
cord-104282-90t1m430	322	39	endoplasmic	endoplasmic	NN
cord-104282-90t1m430	322	40	reticulure	reticulure	NN
cord-104282-90t1m430	322	41	membrane	membrane	NN
cord-104282-90t1m430	322	42	Mechanisms	Mechanisms	NNPS
cord-104282-90t1m430	322	43	for	for	IN
cord-104282-90t1m430	322	44	the	the	DT
cord-104282-90t1m430	322	45	incorporation	incorporation	NN
cord-104282-90t1m430	322	46	of	of	IN
cord-104282-90t1m430	322	47	proteins	protein	NNS
cord-104282-90t1m430	322	48	in	in	IN
cord-104282-90t1m430	322	49	membranes	membrane	NNS
cord-104282-90t1m430	322	50	and	and	CC
cord-104282-90t1m430	322	51	organelles	organelle	NNS
cord-104282-90t1m430	322	52	DNA	dna	NN
cord-104282-90t1m430	322	53	sequencing	sequencing	NN
cord-104282-90t1m430	322	54	with	with	IN
cord-104282-90t1m430	322	55	chain	chain	NN
cord-104282-90t1m430	322	56	-	-	HYPH
cord-104282-90t1m430	322	57	terminating	terminate	VBG
cord-104282-90t1m430	322	58	inhibitors	inhibitor	NNS
cord-104282-90t1m430	323	1	Signals	signal	NNS
cord-104282-90t1m430	323	2	guiding	guide	VBG
cord-104282-90t1m430	323	3	proteins	protein	NNS
cord-104282-90t1m430	323	4	to	to	IN
cord-104282-90t1m430	323	5	their	-PRON-	PRP$
cord-104282-90t1m430	323	6	correct	correct	JJ
cord-104282-90t1m430	323	7	locations	location	NNS
cord-104282-90t1m430	323	8	in	in	IN
cord-104282-90t1m430	323	9	mitochoudria	mitochoudria	NNP
cord-104282-90t1m430	323	10	Cloning	cloning	NN
cord-104282-90t1m430	323	11	and	and	CC
cord-104282-90t1m430	323	12	expression	expression	NN
cord-104282-90t1m430	323	13	ofcDNA	ofcdna	RB
cord-104282-90t1m430	323	14	for	for	IN
cord-104282-90t1m430	323	15	rat	rat	NNP
cord-104282-90t1m430	323	16	heine	heine	NNP
cord-104282-90t1m430	323	17	oxygenase	oxygenase	NNP
cord-104282-90t1m430	323	18	Mechanism	Mechanism	NNP
cord-104282-90t1m430	323	19	of	of	IN
cord-104282-90t1m430	323	20	increase	increase	NN
cord-104282-90t1m430	323	21	of	of	IN
cord-104282-90t1m430	323	22	heine	heine	NNP
cord-104282-90t1m430	323	23	oxygenase	oxygenase	NNP
cord-104282-90t1m430	323	24	by	by	IN
cord-104282-90t1m430	323	25	hemin	hemin	NNP
cord-104282-90t1m430	323	26	in	in	IN
cord-104282-90t1m430	323	27	cultured	cultured	JJ
cord-104282-90t1m430	323	28	pig	pig	NN
cord-104282-90t1m430	323	29	alveolar	alveolar	NN
cord-104282-90t1m430	323	30	macrophages	macrophage	NNS
cord-104282-90t1m430	324	1	The	the	DT
cord-104282-90t1m430	324	2	binding	binding	NN
cord-104282-90t1m430	324	3	of	of	IN
cord-104282-90t1m430	324	4	cytochrome	cytochrome	NN
cord-104282-90t1m430	324	5	b5	b5	NN
cord-104282-90t1m430	324	6	to	to	IN
cord-104282-90t1m430	324	7	liver	liver	NN
cord-104282-90t1m430	324	8	microsomes	microsome	NNS
cord-104282-90t1m430	324	9	A	a	DT
cord-104282-90t1m430	324	10	mouse	mouse	NN
cord-104282-90t1m430	324	11	embryonic	embryonic	JJ
cord-104282-90t1m430	324	12	stem	stem	NN
cord-104282-90t1m430	324	13	cell	cell	NN
cord-104282-90t1m430	324	14	line	line	NN
cord-104282-90t1m430	324	15	showing	show	VBG
cord-104282-90t1m430	324	16	phiripotency	phiripotency	NN
cord-104282-90t1m430	324	17	of	of	IN
cord-104282-90t1m430	324	18	differentiation	differentiation	NN
cord-104282-90t1m430	324	19	in	in	IN
cord-104282-90t1m430	324	20	early	early	JJ
cord-104282-90t1m430	324	21	embryos	embryo	NNS
cord-104282-90t1m430	324	22	and	and	CC
cord-104282-90t1m430	324	23	ubiquitous	ubiquitous	JJ
cord-104282-90t1m430	324	24	~-galactosidase	~-galactosidase	JJ
cord-104282-90t1m430	324	25	expression	expression	NN
cord-104282-90t1m430	325	1	A	a	DT
cord-104282-90t1m430	325	2	Goigl	Goigl	NNP
cord-104282-90t1m430	325	3	retention	retention	NN
cord-104282-90t1m430	325	4	signal	signal	NN
cord-104282-90t1m430	325	5	in	in	IN
cord-104282-90t1m430	325	6	a	a	DT
cord-104282-90t1m430	325	7	membrane	membrane	NN
cord-104282-90t1m430	325	8	-	-	HYPH
cord-104282-90t1m430	325	9	spanning	span	VBG
cord-104282-90t1m430	325	10	domain	domain	NN
cord-104282-90t1m430	325	11	of	of	IN
cord-104282-90t1m430	325	12	coronavirus	coronavirus	NN
cord-104282-90t1m430	325	13	El	El	NNP
cord-104282-90t1m430	325	14	protein	protein	NN
cord-104282-90t1m430	325	15	Biogenesis	biogenesis	NN
cord-104282-90t1m430	325	16	of	of	IN
cord-104282-90t1m430	325	17	microsomal	microsomal	NNP
cord-104282-90t1m430	325	18	aldehyde	aldehyde	NN
cord-104282-90t1m430	325	19	dehydrogenase	dehydrogenase	NN
cord-104282-90t1m430	325	20	in	in	IN
cord-104282-90t1m430	325	21	rat	rat	NN
cord-104282-90t1m430	325	22	liver	liver	NN
cord-104282-90t1m430	325	23	Application	Application	NNP
cord-104282-90t1m430	325	24	of	of	IN
cord-104282-90t1m430	325	25	cryoultramicrotomy	cryoultramicrotomy	NNP
cord-104282-90t1m430	325	26	to	to	IN
cord-104282-90t1m430	325	27	immunocytochemistry	immunocytochemistry	NNP
cord-104282-90t1m430	325	28	Protein	Protein	NNP
cord-104282-90t1m430	325	29	translocatiou	translocatiou	RB
cord-104282-90t1m430	325	30	across	across	IN
cord-104282-90t1m430	325	31	membranes	membrane	NNS
cord-104282-90t1m430	325	32	Biochemical	biochemical	JJ
cord-104282-90t1m430	325	33	transfer	transfer	NN
cord-104282-90t1m430	325	34	of	of	IN
cord-104282-90t1m430	325	35	single	single	JJ
cord-104282-90t1m430	325	36	-	-	HYPH
cord-104282-90t1m430	325	37	copy	copy	NN
cord-104282-90t1m430	325	38	eucaryotic	eucaryotic	JJ
cord-104282-90t1m430	325	39	genes	gene	NNS
cord-104282-90t1m430	325	40	using	use	VBG
cord-104282-90t1m430	325	41	total	total	JJ
cord-104282-90t1m430	325	42	cellular	cellular	JJ
cord-104282-90t1m430	325	43	DNA	dna	NN
cord-104282-90t1m430	325	44	as	as	IN
cord-104282-90t1m430	325	45	donor	donor	NN
cord-104282-90t1m430	326	1	We	-PRON-	PRP
cord-104282-90t1m430	326	2	thank	thank	VBP
cord-104282-90t1m430	326	3	Kimie	Kimie	NNP
cord-104282-90t1m430	326	4	Masaki	Masaki	NNP
cord-104282-90t1m430	326	5	for	for	IN
cord-104282-90t1m430	326	6	valuable	valuable	JJ
cord-104282-90t1m430	326	7	technical	technical	JJ
cord-104282-90t1m430	326	8	assistance	assistance	NN
cord-104282-90t1m430	326	9	,	,	,
cord-104282-90t1m430	326	10	Dr.	Dr.	NNP
cord-104282-90t1m430	326	11	Shigeru	Shigeru	NNP
cord-104282-90t1m430	326	12	Taketani	Taketani	NNP
cord-104282-90t1m430	326	13	for	for	IN
cord-104282-90t1m430	326	14	helpful	helpful	JJ
cord-104282-90t1m430	326	15	comments	comment	NNS
cord-104282-90t1m430	326	16	on	on	IN
cord-104282-90t1m430	326	17	the	the	DT
cord-104282-90t1m430	326	18	manuscript	manuscript	NN
cord-104282-90t1m430	326	19	,	,	,
cord-104282-90t1m430	326	20	Dr.	Dr.	NNP
cord-104282-90t1m430	326	21	Akihiro	Akihiro	NNP
cord-104282-90t1m430	326	22	Inoue	Inoue	NNP
cord-104282-90t1m430	326	23	,	,	,
cord-104282-90t1m430	326	24	National	National	NNP
cord-104282-90t1m430	326	25	Institutes	Institutes	NNPS
cord-104282-90t1m430	326	26	for	for	IN
cord-104282-90t1m430	326	27	Physiological	Physiological	NNP
cord-104282-90t1m430	326	28	Science	Science	NNP
cord-104282-90t1m430	326	29	,	,	,
cord-104282-90t1m430	326	30	Okazaki	Okazaki	NNP
cord-104282-90t1m430	326	31	,	,	,
cord-104282-90t1m430	326	32	Japan	Japan	NNP
cord-104282-90t1m430	326	33	,	,	,
cord-104282-90t1m430	326	34	for	for	IN
cord-104282-90t1m430	326	35	the	the	DT
cord-104282-90t1m430	326	36	generous	generous	JJ
cord-104282-90t1m430	326	37	gift	gift	NN
cord-104282-90t1m430	326	38	of	of	IN
cord-104282-90t1m430	326	39	the	the	DT
cord-104282-90t1m430	326	40	pMIW	pmiw	JJ
cord-104282-90t1m430	326	41	vector	vector	NN
cord-104282-90t1m430	326	42	.	.	.
cord-104282-90t1m430	327	1	This	this	DT
cord-104282-90t1m430	327	2	work	work	NN
cord-104282-90t1m430	327	3	was	be	VBD
cord-104282-90t1m430	327	4	supported	support	VBN
cord-104282-90t1m430	327	5	in	in	IN
cord-104282-90t1m430	327	6	part	part	NN
cord-104282-90t1m430	327	7	by	by	IN
cord-104282-90t1m430	327	8	a	a	DT
cord-104282-90t1m430	327	9	Grant	Grant	NNP
cord-104282-90t1m430	327	10	-	-	HYPH
cord-104282-90t1m430	327	11	in	in	IN
cord-104282-90t1m430	327	12	-	-	HYPH
cord-104282-90t1m430	327	13	Aid	aid	NN
cord-104282-90t1m430	327	14	for	for	IN
cord-104282-90t1m430	327	15	Scientific	Scientific	NNP
cord-104282-90t1m430	327	16	Research	Research	NNP
cord-104282-90t1m430	327	17	from	from	IN
cord-104282-90t1m430	327	18	the	the	DT
cord-104282-90t1m430	327	19	Ministry	Ministry	NNP
cord-104282-90t1m430	327	20	of	of	IN
cord-104282-90t1m430	327	21	Education	Education	NNP
cord-104282-90t1m430	327	22	,	,	,
cord-104282-90t1m430	327	23	Science	Science	NNP
cord-104282-90t1m430	327	24	,	,	,
cord-104282-90t1m430	327	25	and	and	CC
cord-104282-90t1m430	327	26	Culture	Culture	NNP
cord-104282-90t1m430	327	27	of	of	IN
cord-104282-90t1m430	327	28	Japan	Japan	NNP
cord-104282-90t1m430	327	29	,	,	,
cord-104282-90t1m430	327	30	and	and	CC
cord-104282-90t1m430	327	31	by	by	IN
cord-104282-90t1m430	327	32	a	a	DT
cord-104282-90t1m430	327	33	grant	grant	NN
cord-104282-90t1m430	327	34	from	from	IN
cord-104282-90t1m430	327	35	Naito	Naito	NNP
cord-104282-90t1m430	327	36	Foundation	Foundation	NNP
cord-104282-90t1m430	327	37	.	.	.
cord-104282-90t1m430	328	1	Received	receive	VBN
cord-104282-90t1m430	328	2	for	for	IN
cord-104282-90t1m430	328	3	publication	publication	NN
cord-104282-90t1m430	328	4	12	12	CD
cord-104282-90t1m430	328	5	January	January	NNP
cord-104282-90t1m430	328	6	1994	1994	CD
cord-104282-90t1m430	328	7	and	and	CC
cord-104282-90t1m430	328	8	in	in	IN
cord-104282-90t1m430	328	9	revised	revise	VBN
cord-104282-90t1m430	328	10	form	form	NN
cord-104282-90t1m430	328	11	1	1	CD
cord-104282-90t1m430	328	12	June	June	NNP
cord-104282-90t1m430	328	13	1994	1994	CD
cord-104282-90t1m430	328	14	.	.	.
cord-104282-90t1m430	329	1	tide	tide	NNP
cord-104282-90t1m430	329	2	Y	Y	NNP
cord-104282-90t1m430	329	3	with	with	IN
cord-104282-90t1m430	329	4	the	the	DT
cord-104282-90t1m430	329	5	Lys	Lys	NNP
cord-104282-90t1m430	329	6	-	-	HYPH
cord-104282-90t1m430	329	7	Asp	Asp	NNP
cord-104282-90t1m430	329	8	-	-	HYPH
cord-104282-90t1m430	329	9	Glu	Glu	NNP
cord-104282-90t1m430	329	10	-	-	HYPH
cord-104282-90t1m430	329	11	Leu	Leu	NNP
cord-104282-90t1m430	329	12	sequence	sequence	NN
cord-104282-90t1m430	329	13	(	(	-LRB-
cord-104282-90t1m430	329	14	4	4	CD
cord-104282-90t1m430	329	15	)	)	-RRB-
cord-104282-90t1m430	329	16	,	,	,
cord-104282-90t1m430	329	17	thus	thus	RB
cord-104282-90t1m430	329	18	suggesting	suggest	VBG
cord-104282-90t1m430	329	19	the	the	DT
cord-104282-90t1m430	329	20	important	important	JJ
cord-104282-90t1m430	329	21	role	role	NN
cord-104282-90t1m430	329	22	of	of	IN
cord-104282-90t1m430	329	23	the	the	DT
cord-104282-90t1m430	329	24	acidic	acidic	JJ
cord-104282-90t1m430	329	25	Glu	Glu	NNP
cord-104282-90t1m430	329	26	residue	residue	NN
cord-104282-90t1m430	329	27	in	in	IN
cord-104282-90t1m430	329	28	ER	ER	NNP
cord-104282-90t1m430	329	29	retention	retention	NN
cord-104282-90t1m430	329	30	.	.	.
cord-104282-90t1m430	330	1	We	-PRON-	PRP
cord-104282-90t1m430	330	2	are	be	VBP
cord-104282-90t1m430	330	3	now	now	RB
cord-104282-90t1m430	330	4	studying	study	VBG
cord-104282-90t1m430	330	5	the	the	DT
cord-104282-90t1m430	330	6	membrane	membrane	NN
cord-104282-90t1m430	330	7	topology	topology	NN
cord-104282-90t1m430	330	8	of	of	IN
cord-104282-90t1m430	330	9	msALDH	msALDH	NNP
cord-104282-90t1m430	330	10	and	and	CC
cord-104282-90t1m430	330	11	the	the	DT
cord-104282-90t1m430	330	12	intracellular	intracellular	JJ
cord-104282-90t1m430	330	13	localization	localization	NN
cord-104282-90t1m430	330	14	of	of	IN
cord-104282-90t1m430	330	15	both	both	CC
cord-104282-90t1m430	330	16	msALDH	msALDH	NNP
cord-104282-90t1m430	330	17	and	and	CC
cord-104282-90t1m430	330	18	msALDHA481	msaldha481	CD
cord-104282-90t1m430	330	19	-	-	SYM
cord-104282-90t1m430	330	20	484	484	CD
cord-104282-90t1m430	330	21	in	in	IN
cord-104282-90t1m430	330	22	more	more	JJR
cord-104282-90t1m430	330	23	detail	detail	NN
cord-104282-90t1m430	330	24	by	by	IN
cord-104282-90t1m430	330	25	immunoelectron	immunoelectron	NNP
cord-104282-90t1m430	330	26	microscopy	microscopy	NNP
cord-104282-90t1m430	330	27	to	to	TO
cord-104282-90t1m430	330	28	check	check	VB
cord-104282-90t1m430	330	29	the	the	DT
cord-104282-90t1m430	330	30	potential	potential	JJ
cord-104282-90t1m430	330	31	role	role	NN
cord-104282-90t1m430	330	32	of	of	IN
cord-104282-90t1m430	330	33	the	the	DT
cord-104282-90t1m430	330	34	tail	tail	NN
cord-104282-90t1m430	330	35	region	region	NN
cord-104282-90t1m430	330	36	for	for	IN
cord-104282-90t1m430	330	37	ER	ER	NNP
cord-104282-90t1m430	330	38	retention	retention	NN
cord-104282-90t1m430	330	39	.	.	.
cord-104282-90t1m430	331	1	Novel	novel	JJ
cord-104282-90t1m430	331	2	ER	ER	NNP
cord-104282-90t1m430	331	3	targeting	targeting	NN
cord-104282-90t1m430	331	4	sequences	sequence	NNS
cord-104282-90t1m430	331	5	have	have	VBP
cord-104282-90t1m430	331	6	recently	recently	RB
cord-104282-90t1m430	331	7	been	be	VBN
cord-104282-90t1m430	331	8	reported	report	VBN
cord-104282-90t1m430	331	9	for	for	IN
cord-104282-90t1m430	331	10	two	two	CD
cord-104282-90t1m430	331	11	other	other	JJ
cord-104282-90t1m430	331	12	tail	tail	NN
cord-104282-90t1m430	331	13	-	-	HYPH
cord-104282-90t1m430	331	14	anchored	anchor	VBN
cord-104282-90t1m430	331	15	proteins	protein	NNS
cord-104282-90t1m430	331	16	.	.	.
cord-104282-90t1m430	332	1	Frangioni	Frangioni	NNP
cord-104282-90t1m430	332	2	et	et	NNP
cord-104282-90t1m430	332	3	al	al	NNP
cord-104282-90t1m430	332	4	.	.	NNP
cord-104282-90t1m430	332	5	have	have	VBP
cord-104282-90t1m430	332	6	found	find	VBN
cord-104282-90t1m430	332	7	that	that	IN
cord-104282-90t1m430	332	8	the	the	DT
cord-104282-90t1m430	332	9	ER	ER	NNP
cord-104282-90t1m430	332	10	targeting	target	VBG
cord-104282-90t1m430	332	11	sequence	sequence	NN
cord-104282-90t1m430	332	12	of	of	IN
cord-104282-90t1m430	332	13	protein	protein	NN
cord-104282-90t1m430	332	14	tyrosine	tyrosine	NN
cord-104282-90t1m430	332	15	phosphatase	phosphatase	NN
cord-104282-90t1m430	332	16	1B	1b	CD
cord-104282-90t1m430	332	17	(	(	-LRB-
cord-104282-90t1m430	332	18	PTP	PTP	NNP
cord-104282-90t1m430	332	19	1B	1b	CD
cord-104282-90t1m430	332	20	)	)	-RRB-
cord-104282-90t1m430	332	21	exists	exist	VBZ
cord-104282-90t1m430	332	22	within	within	IN
cord-104282-90t1m430	332	23	the	the	DT
cord-104282-90t1m430	332	24	carboxyl	carboxyl	NN
cord-104282-90t1m430	332	25	-	-	HYPH
cord-104282-90t1m430	332	26	terminal	terminal	NN
cord-104282-90t1m430	332	27	35	35	CD
cord-104282-90t1m430	332	28	amino	amino	NN
cord-104282-90t1m430	332	29	acids	acid	NNS
cord-104282-90t1m430	332	30	(	(	-LRB-
cord-104282-90t1m430	332	31	12	12	CD
cord-104282-90t1m430	332	32	)	)	-RRB-
cord-104282-90t1m430	332	33	.	.	.
cord-104282-90t1m430	333	1	Mitoma	Mitoma	NNP
cord-104282-90t1m430	333	2	and	and	CC
cord-104282-90t1m430	333	3	Ito	Ito	NNP
cord-104282-90t1m430	333	4	have	have	VBP
cord-104282-90t1m430	333	5	shown	show	VBN
cord-104282-90t1m430	333	6	that	that	IN
cord-104282-90t1m430	333	7	the	the	DT
cord-104282-90t1m430	333	8	last	last	JJ
cord-104282-90t1m430	333	9	10	10	CD
cord-104282-90t1m430	333	10	amino	amino	NN
cord-104282-90t1m430	333	11	acids	acid	NNS
cord-104282-90t1m430	333	12	of	of	IN
cord-104282-90t1m430	333	13	cytochrome	cytochrome	NN
cord-104282-90t1m430	333	14	b5	b5	NN
cord-104282-90t1m430	333	15	,	,	,
cord-104282-90t1m430	333	16	which	which	WDT
cord-104282-90t1m430	333	17	include	include	VBP
cord-104282-90t1m430	333	18	the	the	DT
cord-104282-90t1m430	333	19	hydrophilic	hydrophilic	JJ
cord-104282-90t1m430	333	20	tail	tail	NN
cord-104282-90t1m430	333	21	of	of	IN
cord-104282-90t1m430	333	22	this	this	DT
cord-104282-90t1m430	333	23	protein	protein	NN
cord-104282-90t1m430	333	24	,	,	,
cord-104282-90t1m430	333	25	are	be	VBP
cord-104282-90t1m430	333	26	important	important	JJ
cord-104282-90t1m430	333	27	for	for	IN
cord-104282-90t1m430	333	28	its	-PRON-	PRP$
cord-104282-90t1m430	333	29	targeting	targeting	NN
cord-104282-90t1m430	333	30	to	to	IN
cord-104282-90t1m430	333	31	the	the	DT
cord-104282-90t1m430	333	32	ER	ER	NNP
cord-104282-90t1m430	333	33	(	(	-LRB-
cord-104282-90t1m430	333	34	23	23	CD
cord-104282-90t1m430	333	35	)	)	-RRB-
cord-104282-90t1m430	333	36	.	.	.
cord-104282-90t1m430	334	1	Fig	Fig	NNP
cord-104282-90t1m430	334	2	.	.	.
cord-104282-90t1m430	335	1	12	12	CD
cord-104282-90t1m430	336	1	A	a	DT
cord-104282-90t1m430	336	2	shows	show	VBZ
cord-104282-90t1m430	336	3	an	an	DT
cord-104282-90t1m430	336	4	alignment	alignment	NN
cord-104282-90t1m430	336	5	of	of	IN
cord-104282-90t1m430	336	6	the	the	DT
cord-104282-90t1m430	336	7	last	last	JJ
cord-104282-90t1m430	336	8	35	35	CD
cord-104282-90t1m430	336	9	amino	amino	NN
cord-104282-90t1m430	336	10	acids	acid	NNS
cord-104282-90t1m430	336	11	of	of	IN
cord-104282-90t1m430	336	12	PTP	PTP	NNP
cord-104282-90t1m430	336	13	1B	1b	NN
cord-104282-90t1m430	336	14	(	(	-LRB-
cord-104282-90t1m430	336	15	9	9	CD
cord-104282-90t1m430	336	16	)	)	-RRB-
cord-104282-90t1m430	336	17	,	,	,
cord-104282-90t1m430	336	18	cytochrome	cytochrome	NN
cord-104282-90t1m430	336	19	b5	b5	NN
cord-104282-90t1m430	336	20	(	(	-LRB-
cord-104282-90t1m430	336	21	32	32	CD
cord-104282-90t1m430	336	22	)	)	-RRB-
cord-104282-90t1m430	336	23	,	,	,
cord-104282-90t1m430	336	24	and	and	CC
cord-104282-90t1m430	336	25	msALDH	msALDH	NNP
cord-104282-90t1m430	336	26	(	(	-LRB-
cord-104282-90t1m430	336	27	24	24	CD
cord-104282-90t1m430	336	28	)	)	-RRB-
cord-104282-90t1m430	336	29	,	,	,
cord-104282-90t1m430	336	30	where	where	WRB
cord-104282-90t1m430	336	31	each	each	DT
cord-104282-90t1m430	336	32	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	336	33	domain	domain	NN
cord-104282-90t1m430	336	34	is	be	VBZ
cord-104282-90t1m430	336	35	underlined	underline	VBN
cord-104282-90t1m430	336	36	and	and	CC
cord-104282-90t1m430	336	37	the	the	DT
cord-104282-90t1m430	336	38	ER	ER	NNP
cord-104282-90t1m430	336	39	-	-	HYPH
cord-104282-90t1m430	336	40	targeting	target	VBG
cord-104282-90t1m430	336	41	sequences	sequence	NNS
cord-104282-90t1m430	336	42	of	of	IN
cord-104282-90t1m430	336	43	cytochrome	cytochrome	NN
cord-104282-90t1m430	336	44	b5	b5	NN
cord-104282-90t1m430	336	45	and	and	CC
cord-104282-90t1m430	336	46	msALDH	msALDH	NNP
cord-104282-90t1m430	336	47	are	be	VBP
cord-104282-90t1m430	336	48	indicated	indicate	VBN
cord-104282-90t1m430	336	49	by	by	IN
cord-104282-90t1m430	336	50	the	the	DT
cord-104282-90t1m430	336	51	broken	broken	JJ
cord-104282-90t1m430	336	52	line	line	NN
cord-104282-90t1m430	336	53	.	.	.
cord-104282-90t1m430	337	1	Although	although	IN
cord-104282-90t1m430	337	2	the	the	DT
cord-104282-90t1m430	337	3	sequence	sequence	NN
cord-104282-90t1m430	337	4	required	require	VBN
cord-104282-90t1m430	337	5	for	for	IN
cord-104282-90t1m430	337	6	ER	ER	NNP
cord-104282-90t1m430	337	7	targeting	target	VBG
cord-104282-90t1m430	337	8	within	within	IN
cord-104282-90t1m430	337	9	the	the	DT
cord-104282-90t1m430	337	10	last	last	JJ
cord-104282-90t1m430	337	11	35	35	CD
cord-104282-90t1m430	337	12	amino	amino	NN
cord-104282-90t1m430	337	13	acids	acid	NNS
cord-104282-90t1m430	337	14	of	of	IN
cord-104282-90t1m430	337	15	PTP	PTP	NNP
cord-104282-90t1m430	337	16	1B	1b	NN
cord-104282-90t1m430	337	17	has	have	VBZ
cord-104282-90t1m430	337	18	not	not	RB
cord-104282-90t1m430	337	19	been	be	VBN
cord-104282-90t1m430	337	20	defined	define	VBN
cord-104282-90t1m430	337	21	,	,	,
cord-104282-90t1m430	337	22	PTP	PTP	NNP
cord-104282-90t1m430	337	23	1B	1b	NN
cord-104282-90t1m430	337	24	and	and	CC
cord-104282-90t1m430	337	25	cytochrome	cytochrome	NN
cord-104282-90t1m430	337	26	b5	b5	NNP
cord-104282-90t1m430	337	27	have	have	VBP
cord-104282-90t1m430	337	28	the	the	DT
cord-104282-90t1m430	337	29	common	common	JJ
cord-104282-90t1m430	337	30	Leu	Leu	NNP
cord-104282-90t1m430	337	31	-	-	HYPH
cord-104282-90t1m430	337	32	X	X	NNP
cord-104282-90t1m430	337	33	-	-	HYPH
cord-104282-90t1m430	337	34	Tyr	Tyr	NNP
cord-104282-90t1m430	337	35	-	-	HYPH
cord-104282-90t1m430	337	36	Arg	arg	NN
cord-104282-90t1m430	337	37	motif	motif	NN
cord-104282-90t1m430	337	38	(	(	-LRB-
cord-104282-90t1m430	337	39	Fig	fig	NN
cord-104282-90t1m430	337	40	.	.	.
cord-104282-90t1m430	337	41	12	12	CD
cord-104282-90t1m430	337	42	A	a	DT
cord-104282-90t1m430	337	43	)	)	-RRB-
cord-104282-90t1m430	337	44	within	within	IN
cord-104282-90t1m430	337	45	their	-PRON-	PRP$
cord-104282-90t1m430	337	46	last	last	JJ
cord-104282-90t1m430	337	47	10	10	CD
cord-104282-90t1m430	337	48	amino	amino	NN
cord-104282-90t1m430	337	49	acids	acid	NNS
cord-104282-90t1m430	337	50	.	.	.
cord-104282-90t1m430	338	1	In	in	IN
cord-104282-90t1m430	338	2	addition	addition	NN
cord-104282-90t1m430	338	3	,	,	,
cord-104282-90t1m430	338	4	PTP	PTP	NNP
cord-104282-90t1m430	338	5	1B	1b	NN
cord-104282-90t1m430	338	6	and	and	CC
cord-104282-90t1m430	338	7	cytochrome	cytochrome	NN
cord-104282-90t1m430	338	8	b5	b5	NNP
cord-104282-90t1m430	338	9	have	have	VBP
cord-104282-90t1m430	338	10	tails	tail	NNS
cord-104282-90t1m430	338	11	of	of	IN
cord-104282-90t1m430	338	12	similar	similar	JJ
cord-104282-90t1m430	338	13	length	length	NN
cord-104282-90t1m430	338	14	(	(	-LRB-
cord-104282-90t1m430	338	15	seven	seven	CD
cord-104282-90t1m430	338	16	and	and	CC
cord-104282-90t1m430	338	17	eight	eight	CD
cord-104282-90t1m430	338	18	amino	amino	NN
cord-104282-90t1m430	338	19	acid	acid	NN
cord-104282-90t1m430	338	20	residues	residue	NNS
cord-104282-90t1m430	338	21	,	,	,
cord-104282-90t1m430	338	22	respectively	respectively	RB
cord-104282-90t1m430	338	23	)	)	-RRB-
cord-104282-90t1m430	338	24	,	,	,
cord-104282-90t1m430	338	25	which	which	WDT
cord-104282-90t1m430	338	26	are	be	VBP
cord-104282-90t1m430	338	27	longer	long	JJR
cord-104282-90t1m430	338	28	than	than	IN
cord-104282-90t1m430	338	29	that	that	DT
cord-104282-90t1m430	339	1	(	(	-LRB-
cord-104282-90t1m430	339	2	four	four	CD
cord-104282-90t1m430	339	3	amino	amino	NN
cord-104282-90t1m430	339	4	acid	acid	NN
cord-104282-90t1m430	339	5	residues	residue	NNS
cord-104282-90t1m430	339	6	)	)	-RRB-
cord-104282-90t1m430	339	7	of	of	IN
cord-104282-90t1m430	339	8	msALDH	msALDH	NNP
cord-104282-90t1m430	339	9	.	.	.
cord-104282-90t1m430	340	1	It	-PRON-	PRP
cord-104282-90t1m430	340	2	therefore	therefore	RB
cord-104282-90t1m430	340	3	seems	seem	VBZ
cord-104282-90t1m430	340	4	likely	likely	JJ
cord-104282-90t1m430	340	5	that	that	IN
cord-104282-90t1m430	340	6	PTP	PTP	NNP
cord-104282-90t1m430	340	7	1B	1b	NN
cord-104282-90t1m430	340	8	possesses	possess	VBZ
cord-104282-90t1m430	340	9	a	a	DT
cord-104282-90t1m430	340	10	similar	similar	JJ
cord-104282-90t1m430	340	11	ER	er	NN
cord-104282-90t1m430	340	12	-	-	HYPH
cord-104282-90t1m430	340	13	targeting	target	VBG
cord-104282-90t1m430	340	14	sequence	sequence	NN
cord-104282-90t1m430	340	15	to	to	IN
cord-104282-90t1m430	340	16	that	that	DT
cord-104282-90t1m430	340	17	of	of	IN
cord-104282-90t1m430	340	18	cytochrome	cytochrome	NN
cord-104282-90t1m430	340	19	b5	b5	NN
cord-104282-90t1m430	340	20	.	.	.
cord-104282-90t1m430	341	1	We	-PRON-	PRP
cord-104282-90t1m430	341	2	have	have	VBP
cord-104282-90t1m430	341	3	not	not	RB
cord-104282-90t1m430	341	4	found	find	VBN
cord-104282-90t1m430	341	5	in	in	IN
cord-104282-90t1m430	341	6	the	the	DT
cord-104282-90t1m430	341	7	carboxyl	carboxyl	NN
cord-104282-90t1m430	341	8	-	-	HYPH
cord-104282-90t1m430	341	9	terminal	terminal	NN
cord-104282-90t1m430	341	10	portion	portion	NN
cord-104282-90t1m430	341	11	of	of	IN
cord-104282-90t1m430	341	12	msALDH	msALDH	NNP
cord-104282-90t1m430	341	13	a	a	DT
cord-104282-90t1m430	341	14	motif	motif	NN
cord-104282-90t1m430	341	15	similar	similar	JJ
cord-104282-90t1m430	341	16	to	to	IN
cord-104282-90t1m430	341	17	that	that	DT
cord-104282-90t1m430	341	18	in	in	IN
cord-104282-90t1m430	341	19	those	those	DT
cord-104282-90t1m430	341	20	two	two	CD
cord-104282-90t1m430	341	21	proteins	protein	NNS
cord-104282-90t1m430	341	22	,	,	,
cord-104282-90t1m430	341	23	despite	despite	IN
cord-104282-90t1m430	341	24	the	the	DT
cord-104282-90t1m430	341	25	fact	fact	NN
cord-104282-90t1m430	341	26	that	that	IN
cord-104282-90t1m430	341	27	the	the	DT
cord-104282-90t1m430	341	28	ERtargeting	ERtargeting	NNP
cord-104282-90t1m430	341	29	sequences	sequence	NNS
cord-104282-90t1m430	341	30	of	of	IN
cord-104282-90t1m430	341	31	cytochrome	cytochrome	NN
cord-104282-90t1m430	341	32	b5	b5	NN
cord-104282-90t1m430	341	33	and	and	CC
cord-104282-90t1m430	341	34	msALDH	msALDH	NNP
cord-104282-90t1m430	341	35	are	be	VBP
cord-104282-90t1m430	341	36	both	both	DT
cord-104282-90t1m430	341	37	hydrophilic	hydrophilic	JJ
cord-104282-90t1m430	341	38	and	and	CC
cord-104282-90t1m430	341	39	adjacent	adjacent	JJ
cord-104282-90t1m430	341	40	to	to	IN
cord-104282-90t1m430	341	41	the	the	DT
cord-104282-90t1m430	341	42	membrane	membrane	NN
cord-104282-90t1m430	341	43	anchors	anchor	NNS
cord-104282-90t1m430	341	44	.	.	.
cord-104282-90t1m430	342	1	In	in	IN
cord-104282-90t1m430	342	2	addition	addition	NN
cord-104282-90t1m430	342	3	,	,	,
cord-104282-90t1m430	342	4	the	the	DT
cord-104282-90t1m430	342	5	presence	presence	NN
cord-104282-90t1m430	342	6	in	in	IN
cord-104282-90t1m430	342	7	msALDH	msALDH	NNP
cord-104282-90t1m430	342	8	of	of	IN
cord-104282-90t1m430	342	9	two	two	CD
cord-104282-90t1m430	342	10	ER	ER	NNP
cord-104282-90t1m430	342	11	-	-	HYPH
cord-104282-90t1m430	342	12	targeting	targeting	JJ
cord-104282-90t1m430	342	13	sequences	sequence	NNS
cord-104282-90t1m430	342	14	separated	separate	VBN
cord-104282-90t1m430	342	15	by	by	IN
cord-104282-90t1m430	342	16	the	the	DT
cord-104282-90t1m430	342	17	membrane	membrane	NN
cord-104282-90t1m430	342	18	-	-	HYPH
cord-104282-90t1m430	342	19	anchoring	anchor	VBG
cord-104282-90t1m430	342	20	domain	domain	NN
cord-104282-90t1m430	342	21	suggests	suggest	VBZ
cord-104282-90t1m430	342	22	that	that	IN
cord-104282-90t1m430	342	23	this	this	DT
cord-104282-90t1m430	342	24	protein	protein	NN
cord-104282-90t1m430	342	25	,	,	,
cord-104282-90t1m430	342	26	with	with	IN
cord-104282-90t1m430	342	27	respect	respect	NN
cord-104282-90t1m430	342	28	to	to	IN
cord-104282-90t1m430	342	29	the	the	DT
cord-104282-90t1m430	342	30	ER	ER	NNP
cord-104282-90t1m430	342	31	-	-	HYPH
cord-104282-90t1m430	342	32	targeting	target	VBG
cord-104282-90t1m430	342	33	sequence	sequence	NN
cord-104282-90t1m430	342	34	,	,	,
cord-104282-90t1m430	342	35	belongs	belong	VBZ
cord-104282-90t1m430	342	36	to	to	IN
cord-104282-90t1m430	342	37	a	a	DT
cord-104282-90t1m430	342	38	different	different	JJ
cord-104282-90t1m430	342	39	class	class	NN
cord-104282-90t1m430	342	40	than	than	IN
cord-104282-90t1m430	342	41	the	the	DT
cord-104282-90t1m430	342	42	other	other	JJ
cord-104282-90t1m430	342	43	two	two	CD
cord-104282-90t1m430	342	44	.	.	.
cord-104282-90t1m430	343	1	In	in	IN
cord-104282-90t1m430	343	2	addition	addition	NN
cord-104282-90t1m430	343	3	to	to	IN
cord-104282-90t1m430	343	4	the	the	DT
cord-104282-90t1m430	343	5	three	three	CD
cord-104282-90t1m430	343	6	proteins	protein	NNS
cord-104282-90t1m430	343	7	described	describe	VBN
cord-104282-90t1m430	343	8	above	above	RB
cord-104282-90t1m430	343	9	,	,	,
cord-104282-90t1m430	343	10	several	several	JJ
cord-104282-90t1m430	343	11	other	other	JJ
cord-104282-90t1m430	343	12	ER	ER	NNP
cord-104282-90t1m430	343	13	membrane	membrane	NN
cord-104282-90t1m430	343	14	proteins	protein	NNS
cord-104282-90t1m430	343	15	are	be	VBP
cord-104282-90t1m430	343	16	known	know	VBN
cord-104282-90t1m430	343	17	that	that	WDT
cord-104282-90t1m430	343	18	also	also	RB
cord-104282-90t1m430	343	19	have	have	VBP
cord-104282-90t1m430	343	20	carboxyl	carboxyl	NN
cord-104282-90t1m430	343	21	-	-	HYPH
cord-104282-90t1m430	343	22	terminal	terminal	NN
cord-104282-90t1m430	343	23	anchors	anchor	NNS
cord-104282-90t1m430	343	24	,	,	,
cord-104282-90t1m430	343	25	such	such	JJ
cord-104282-90t1m430	343	26	as	as	IN
cord-104282-90t1m430	343	27	heine	heine	NNP
cord-104282-90t1m430	343	28	oxygenase	oxygenase	NNP
cord-104282-90t1m430	343	29	(	(	-LRB-
cord-104282-90t1m430	343	30	40	40	CD
cord-104282-90t1m430	343	31	)	)	-RRB-
cord-104282-90t1m430	343	32	and	and	CC
cord-104282-90t1m430	343	33	ribosome	ribosome	NN
cord-104282-90t1m430	343	34	-	-	HYPH
cord-104282-90t1m430	343	35	binding	bind	VBG
cord-104282-90t1m430	343	36	protein	protein	NN
cord-104282-90t1m430	343	37	p34	p34	CD
cord-104282-90t1m430	343	38	(	(	-LRB-
cord-104282-90t1m430	343	39	28	28	CD
cord-104282-90t1m430	343	40	)	)	-RRB-
cord-104282-90t1m430	343	41	,	,	,
cord-104282-90t1m430	343	42	but	but	CC
cord-104282-90t1m430	343	43	ER	er	NN
cord-104282-90t1m430	343	44	-	-	HYPH
cord-104282-90t1m430	343	45	targeting	target	VBG
cord-104282-90t1m430	343	46	sequences	sequence	NNS
cord-104282-90t1m430	343	47	of	of	IN
cord-104282-90t1m430	343	48	these	these	DT
cord-104282-90t1m430	343	49	proteins	protein	NNS
cord-104282-90t1m430	343	50	have	have	VBP
cord-104282-90t1m430	343	51	not	not	RB
cord-104282-90t1m430	343	52	yet	yet	RB
cord-104282-90t1m430	343	53	been	be	VBN
cord-104282-90t1m430	343	54	identified	identify	VBN
cord-104282-90t1m430	343	55	.	.	.
cord-104282-90t1m430	344	1	Cytochrome	cytochrome	NN
cord-104282-90t1m430	344	2	bS	bS	NNP
cord-104282-90t1m430	344	3	,	,	,
cord-104282-90t1m430	344	4	heme	heme	NN
cord-104282-90t1m430	344	5	oxygenase	oxygenase	NN
cord-104282-90t1m430	344	6	,	,	,
cord-104282-90t1m430	344	7	and	and	CC
cord-104282-90t1m430	344	8	msALDH	msALDH	NNP
cord-104282-90t1m430	344	9	are	be	VBP
cord-104282-90t1m430	344	10	synthesized	synthesize	VBN
cord-104282-90t1m430	344	11	on	on	IN
cord-104282-90t1m430	344	12	free	free	JJ
cord-104282-90t1m430	344	13	polysomes	polysome	NNS
cord-104282-90t1m430	344	14	(	(	-LRB-
cord-104282-90t1m430	344	15	29	29	CD
cord-104282-90t1m430	344	16	,	,	,
cord-104282-90t1m430	344	17	41	41	CD
cord-104282-90t1m430	344	18	,	,	,
cord-104282-90t1m430	344	19	45	45	CD
cord-104282-90t1m430	344	20	)	)	-RRB-
cord-104282-90t1m430	344	21	and	and	CC
cord-104282-90t1m430	344	22	posttranslationally	posttranslationally	RB
cord-104282-90t1m430	344	23	targeted	target	VBN
cord-104282-90t1m430	344	24	to	to	IN
cord-104282-90t1m430	344	25	the	the	DT
cord-104282-90t1m430	344	26	ER	ER	NNP
cord-104282-90t1m430	344	27	.	.	.
cord-104282-90t1m430	345	1	Indeed	indeed	RB
cord-104282-90t1m430	345	2	,	,	,
cord-104282-90t1m430	345	3	the	the	DT
cord-104282-90t1m430	345	4	SRP	SRP	NNP
cord-104282-90t1m430	345	5	-	-	HYPH
cord-104282-90t1m430	345	6	independent	independent	JJ
cord-104282-90t1m430	345	7	integration	integration	NN
cord-104282-90t1m430	345	8	of	of	IN
cord-104282-90t1m430	345	9	cytochrome	cytochrome	NN
cord-104282-90t1m430	345	10	b5	b5	NN
cord-104282-90t1m430	345	11	into	into	IN
cord-104282-90t1m430	345	12	microsomal	microsomal	NNP
cord-104282-90t1m430	345	13	membranes	membrane	NNS
cord-104282-90t1m430	345	14	has	have	VBZ
cord-104282-90t1m430	345	15	been	be	VBN
cord-104282-90t1m430	345	16	demonstrated	demonstrate	VBN
cord-104282-90t1m430	345	17	(	(	-LRB-
cord-104282-90t1m430	345	18	3	3	CD
cord-104282-90t1m430	345	19	)	)	-RRB-
cord-104282-90t1m430	345	20	.	.	.
cord-104282-90t1m430	346	1	Our	-PRON-	PRP$
cord-104282-90t1m430	346	2	present	present	JJ
cord-104282-90t1m430	346	3	study	study	NN
cord-104282-90t1m430	346	4	,	,	,
cord-104282-90t1m430	346	5	together	together	RB
cord-104282-90t1m430	346	6	with	with	IN
cord-104282-90t1m430	346	7	these	these	DT
cord-104282-90t1m430	346	8	findings	finding	NNS
cord-104282-90t1m430	346	9	,	,	,
cord-104282-90t1m430	346	10	suggests	suggest	VBZ
cord-104282-90t1m430	346	11	a	a	DT
cord-104282-90t1m430	346	12	novel	novel	JJ
cord-104282-90t1m430	346	13	pathway	pathway	NN
cord-104282-90t1m430	346	14	by	by	IN
cord-104282-90t1m430	346	15	which	which	WDT
cord-104282-90t1m430	346	16	a	a	DT
cord-104282-90t1m430	346	17	class	class	NN
cord-104282-90t1m430	346	18	of	of	IN
cord-104282-90t1m430	346	19	tailanchored	tailanchored	JJ
cord-104282-90t1m430	346	20	proteins	protein	NNS
cord-104282-90t1m430	346	21	with	with	IN
cord-104282-90t1m430	346	22	a	a	DT
cord-104282-90t1m430	346	23	novel	novel	JJ
cord-104282-90t1m430	346	24	targeting	targeting	NN
cord-104282-90t1m430	346	25	sequence	sequence	NN
cord-104282-90t1m430	346	26	is	be	VBZ
cord-104282-90t1m430	346	27	directed	direct	VBN
cord-104282-90t1m430	346	28	to	to	IN
cord-104282-90t1m430	346	29	the	the	DT
cord-104282-90t1m430	346	30	ER.How	ER.How	NNP
cord-104282-90t1m430	347	1	might	may	MD
cord-104282-90t1m430	347	2	the	the	DT
cord-104282-90t1m430	347	3	ER	ER	NNP
cord-104282-90t1m430	347	4	-	-	HYPH
cord-104282-90t1m430	347	5	targeting	target	VBG
cord-104282-90t1m430	347	6	sequence	sequence	NN
cord-104282-90t1m430	347	7	function	function	NN
cord-104282-90t1m430	347	8	?	?	.
cord-104282-90t1m430	348	1	Fig	Fig	NNP
cord-104282-90t1m430	348	2	.	.	.
cord-104282-90t1m430	349	1	12	12	CD
cord-104282-90t1m430	349	2	B	b	NN
cord-104282-90t1m430	349	3	shows	show	VBZ
cord-104282-90t1m430	349	4	two	two	CD
cord-104282-90t1m430	349	5	possible	possible	JJ
cord-104282-90t1m430	349	6	models	model	NNS
cord-104282-90t1m430	349	7	to	to	TO
cord-104282-90t1m430	349	8	explain	explain	VB
cord-104282-90t1m430	349	9	the	the	DT
cord-104282-90t1m430	349	10	ER	ER	NNP
cord-104282-90t1m430	349	11	targeting	targeting	NN
cord-104282-90t1m430	349	12	of	of	IN
cord-104282-90t1m430	349	13	msALDH	msALDH	NNP
cord-104282-90t1m430	349	14	.	.	.
cord-104282-90t1m430	350	1	One	one	CD
cord-104282-90t1m430	350	2	possibility	possibility	NN
cord-104282-90t1m430	350	3	(	(	-LRB-
cord-104282-90t1m430	350	4	/	/	NFP
cord-104282-90t1m430	350	5	)	)	-RRB-
cord-104282-90t1m430	350	6	is	be	VBZ
cord-104282-90t1m430	350	7	that	that	IN
cord-104282-90t1m430	350	8	the	the	DT
cord-104282-90t1m430	350	9	hydrophilic	hydrophilic	JJ
cord-104282-90t1m430	350	10	targeting	targeting	NN
cord-104282-90t1m430	350	11	sequences	sequence	NNS
cord-104282-90t1m430	350	12	serve	serve	VBP
cord-104282-90t1m430	350	13	to	to	TO
cord-104282-90t1m430	350	14	prevent	prevent	VB
cord-104282-90t1m430	350	15	the	the	DT
cord-104282-90t1m430	350	16	aggregation	aggregation	NN
cord-104282-90t1m430	350	17	of	of	IN
cord-104282-90t1m430	350	18	the	the	DT
cord-104282-90t1m430	350	19	newly	newly	RB
cord-104282-90t1m430	350	20	synthesized	synthesize	VBN
cord-104282-90t1m430	350	21	msALDH	msaldh	NN
cord-104282-90t1m430	350	22	polypeptides	polypeptide	NNS
cord-104282-90t1m430	350	23	through	through	IN
cord-104282-90t1m430	350	24	their	-PRON-	PRP$
cord-104282-90t1m430	350	25	hydrophobic	hydrophobic	JJ
cord-104282-90t1m430	350	26	anchoring	anchoring	NN
cord-104282-90t1m430	350	27	sequences	sequence	NNS
cord-104282-90t1m430	350	28	,	,	,
cord-104282-90t1m430	350	29	which	which	WDT
cord-104282-90t1m430	350	30	makes	make	VBZ
cord-104282-90t1m430	350	31	it	-PRON-	PRP
cord-104282-90t1m430	350	32	easier	easy	JJR
cord-104282-90t1m430	350	33	for	for	IN
cord-104282-90t1m430	350	34	them	-PRON-	PRP
cord-104282-90t1m430	350	35	finding	find	VBG
cord-104282-90t1m430	350	36	a.	a.	NN
cord-104282-90t1m430	350	37	specific	specific	JJ
cord-104282-90t1m430	350	38	receptor	receptor	NN
cord-104282-90t1m430	350	39	on	on	IN
cord-104282-90t1m430	350	40	the	the	DT
cord-104282-90t1m430	350	41	ER	ER	NNP
cord-104282-90t1m430	350	42	membrane	membrane	NN
cord-104282-90t1m430	350	43	.	.	.
cord-104282-90t1m430	351	1	Another	another	DT
cord-104282-90t1m430	351	2	possibility	possibility	NN
cord-104282-90t1m430	351	3	(	(	-LRB-
cord-104282-90t1m430	351	4	I/	I/	NNP
cord-104282-90t1m430	351	5	)	)	-RRB-
cord-104282-90t1m430	351	6	is	be	VBZ
cord-104282-90t1m430	351	7	that	that	IN
cord-104282-90t1m430	351	8	a	a	DT
cord-104282-90t1m430	351	9	cytosolic	cytosolic	JJ
cord-104282-90t1m430	351	10	receptor	receptor	NN
cord-104282-90t1m430	351	11	binds	bind	VBZ
cord-104282-90t1m430	351	12	to	to	IN
cord-104282-90t1m430	351	13	the	the	DT
cord-104282-90t1m430	351	14	targeting	target	VBG
cord-104282-90t1m430	351	15	sequences	sequence	NNS
cord-104282-90t1m430	351	16	of	of	IN
cord-104282-90t1m430	351	17	msALDH	msALDH	NNP
cord-104282-90t1m430	351	18	to	to	TO
cord-104282-90t1m430	351	19	form	form	VB
cord-104282-90t1m430	351	20	a	a	DT
cord-104282-90t1m430	351	21	complex	complex	NN
cord-104282-90t1m430	351	22	that	that	WDT
cord-104282-90t1m430	351	23	effects	effect	VBZ
cord-104282-90t1m430	351	24	the	the	DT
cord-104282-90t1m430	351	25	correct	correct	JJ
cord-104282-90t1m430	351	26	insertion	insertion	NN
cord-104282-90t1m430	351	27	of	of	IN
cord-104282-90t1m430	351	28	the	the	DT
cord-104282-90t1m430	351	29	carboxyl	carboxyl	NN
cord-104282-90t1m430	351	30	-	-	HYPH
cord-104282-90t1m430	351	31	terminal	terminal	NN
cord-104282-90t1m430	351	32	anchor	anchor	NN
cord-104282-90t1m430	351	33	into	into	IN
cord-104282-90t1m430	351	34	the	the	DT
cord-104282-90t1m430	351	35	ER	ER	NNP
cord-104282-90t1m430	351	36	membrane	membrane	NN
cord-104282-90t1m430	351	37	spontaneously	spontaneously	RB
cord-104282-90t1m430	351	38	or	or	CC
cord-104282-90t1m430	351	39	through	through	IN
cord-104282-90t1m430	351	40	their	-PRON-	PRP$
cord-104282-90t1m430	351	41	interactions	interaction	NNS
cord-104282-90t1m430	351	42	with	with	IN
cord-104282-90t1m430	351	43	a	a	DT
cord-104282-90t1m430	351	44	receptor	receptor	NN
cord-104282-90t1m430	351	45	on	on	IN
cord-104282-90t1m430	351	46	the	the	DT
cord-104282-90t1m430	351	47	membrane	membrane	NN
cord-104282-90t1m430	351	48	.	.	.
cord-104282-90t1m430	352	1	The	the	DT
cord-104282-90t1m430	352	2	elucidation	elucidation	NN
cord-104282-90t1m430	352	3	of	of	IN
cord-104282-90t1m430	352	4	the	the	DT
cord-104282-90t1m430	352	5	mechanisms	mechanism	NNS
cord-104282-90t1m430	352	6	by	by	IN
cord-104282-90t1m430	352	7	which	which	WDT
cord-104282-90t1m430	352	8	ER	er	NN
cord-104282-90t1m430	352	9	localization	localization	NN
cord-104282-90t1m430	352	10	of	of	IN
cord-104282-90t1m430	352	11	msALDH	msaldh	NN
cord-104282-90t1m430	352	12	occurs	occur	VBZ
cord-104282-90t1m430	352	13	should	should	MD
cord-104282-90t1m430	352	14	provide	provide	VB
cord-104282-90t1m430	352	15	valuable	valuable	JJ
cord-104282-90t1m430	352	16	clues	clue	NNS
cord-104282-90t1m430	352	17	for	for	IN
cord-104282-90t1m430	352	18	an	an	DT
cord-104282-90t1m430	352	19	understanding	understanding	NN
cord-104282-90t1m430	352	20	of	of	IN
cord-104282-90t1m430	352	21	the	the	DT
cord-104282-90t1m430	352	22	posttranslational	posttranslational	JJ
cord-104282-90t1m430	352	23	targeting	targeting	NN
cord-104282-90t1m430	352	24	and	and	CC
cord-104282-90t1m430	352	25	insertion	insertion	NN
cord-104282-90t1m430	352	26	of	of	IN
cord-104282-90t1m430	352	27	a	a	DT
cord-104282-90t1m430	352	28	class	class	NN
cord-104282-90t1m430	352	29	of	of	IN
cord-104282-90t1m430	352	30	membrane	membrane	NN
cord-104282-90t1m430	352	31	proteins	protein	NNS
cord-104282-90t1m430	352	32	with	with	IN
cord-104282-90t1m430	352	33	a	a	DT
cord-104282-90t1m430	352	34	carboxyl	carboxyl	NN
cord-104282-90t1m430	352	35	-	-	HYPH
cord-104282-90t1m430	352	36	terminal	terminal	NN
cord-104282-90t1m430	352	37	anchor	anchor	NN
cord-104282-90t1m430	352	38	.	.	.