id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001134-8ljgxnhf	Lin, Chao-Nan	Comparison of viremia of type II porcine reproductive and respiratory syndrome virus in naturally infected pigs by zip nucleic acid probe-based real-time PCR	2013-09-12		.txt	text/plain	2978	165	52	title: Comparison of viremia of type II porcine reproductive and respiratory syndrome virus in naturally infected pigs by zip nucleic acid probe-based real-time PCR In this study, we developed a sensitive and specific zip nucleic acid probe-based real-time PCR assay to evaluate the viremia of natural PRRSV-infected pigs in Taiwan. CONCLUSIONS: ZNA probe-based real-time PCR can be a useful tool to diagnose symptomatic and asymptomatic PRRSV-infected pigs. ZNA probe-based real-time PCR amplification and the limit of detection Tenfold serial plasmid dilutions (10 1 to 10 6 copies/μl) were tested and used to construct the standard curve by plotting the logarithm of the plasmid copy number against the measured quantification cycles (Cq) values. However, this is first study to report the viral load using serum samples in asymptomatic PRRSV-infected pigs using ZNA probebased real-time PCR. Development of a onestep real-time quantitative PCR assay based on primer-probe energy transfer for the detection of porcine reproductive and respiratory syndrome virus	./cache/cord-001134-8ljgxnhf.txt	./txt/cord-001134-8ljgxnhf.txt