id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001371-wf0vonkn	Xiao, Shuqi	Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR	2014-06-12		.txt	text/plain	2767	133	58	The real-time RT-PCR developed based on SYBR Green and TaqMan probe could be used for simultaneous detection and differentiation of HP-PRRSV and PRRSV in China, which will benefit much the PRRS control and research. In this research, the real-time RT-PCR for simultaneous detection and differentiation of HP-PRRSV and PRRSV by using both SYBR Green and TaqMan probe was developed and validated. Our results showed that real-time PCR using both SYBR Green I and TaqMan probe could be used to simultaneously detect and differentiate HP-PRRSV and PRRSV in China. The real-time PCR developed based on SYBR Green and TaqMan probe could be used for simultaneous detection and differentiation of HP-PRRSV and PRRSV in China, which provided two alternative diagnostic assays in diverse PRRSV epidemiological circumstances. Simultaneous detection and genotyping of porcine reproductive and respiratory syndrome virus (PRRSV) by real-time RT-PCR and amplicon melting curve analysis using SYBR Green	./cache/cord-001371-wf0vonkn.txt	./txt/cord-001371-wf0vonkn.txt