id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004151-9815ikzg	Pan, Xiaocheng	Illumination of PRRSV Cytotoxic T Lymphocyte Epitopes by the Three-Dimensional Structure and Peptidome of Swine Lymphocyte Antigen Class I (SLA-I)	2020-01-08		.txt	text/plain	6365	371	56	To investigate CTL epitope applications in swine, SLA-1(*)1502-restricted peptide epitopes matching porcine reproductive and respiratory syndrome virus (PRRSV) strains were explored by crystallography, biochemistry, and the specific pathogen-free (SPF) swine experiments. Next, the potential SLA-1(*)1502-restricted peptide epitopes matching four typical genetic PRRSV strains were identified based on the peptide-binding motif of SLA-1(*)1502 determined by structural analysis and alanine scanning of the NSP9-TMP9 peptide. In an attempt to identify anti-PRRSV CTL epitopes in this study, first, predicted peptide epitopes derived from PRRSV were synthesized, and a trimolecular complex, the structure of the epitope from PRRSV-NSP9 (TMPPGFELY, termed NSP9-TMP9)-bound SLA-1 * 1502 (pSLA-1 * 1502), was solved. The purified complex (44 kDa) of pSLA-1 * 1502 with the NSP9-TMP9 peptide (amino acid sequence TMPPGFELY, derived from residues 198-206 of the PRRSV non-structural protein) was dialyzed against crystallization buffer (20 mM Tris-HCl pH 8.0, 50 mM NaCl) and concentrated to 12 mg/mL.	./cache/cord-004151-9815ikzg.txt	./txt/cord-004151-9815ikzg.txt