id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-005376-tzmettky	Lee, Jung-Ah	Development of a chimeric strain of porcine reproductive and respiratory syndrome virus with an infectious clone and a Korean dominant field strain	2014-03-29		.txt	text/plain	1986	100	51	The K418 chimeric virus of porcine reproductive and respiratory syndrome virus (PRRSV) was engineered by replacing the genomic region containing structure protein genes of an infectious clone of PRRSV, FL12, with the same region obtained from a Korean dominant field strain, LMY. In previous studies, diverse chimera viruses of PRRSV have been generated using RG technology to manipulate viral genomes to determine the viral protein involved in the pathogenicity of field strains in pigs (Kwon et al., 2006; Zhou et al., 2012; Ni et al., 2013) . In this study, a chimeric virus was constructed using an infectious clone of PRRSV containing genomes of the FL12 strain and a Korean dominant field strain, LMY. To generate customized vaccine candidate, the genomic region of established PRRSV infectious clone encoding structure proteins that play a critical role in the virus neutralizing response were replaced with the same genomic region from a Korean dominant field strain of PRRSV.	./cache/cord-005376-tzmettky.txt	./txt/cord-005376-tzmettky.txt