id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007476-wu9tuvy9	Katz, Jonathan B.	Antigenic differences between European and American isolates of porcine reproductive and respiratory syndrome virus (PRRSV) are encoded by the carboxyterminal portion of viral open reading frame 3	2000-03-10		.txt	text/plain	3895	177	39	title: Antigenic differences between European and American isolates of porcine reproductive and respiratory syndrome virus (PRRSV) are encoded by the carboxyterminal portion of viral open reading frame 3 Antigenic differences between European and American isolates of porcine reproductive and respiratory syndrome virus (PRRSV) were revealed by serologic analysis of a recombinant protein derived from PRRSV open reading frame 3 (ORF 3). Sera from rabbits inoculated with BP03-P failed to neutralize both the European (Lelystad) and American (ATCC VR-2332) reference isolates of PRRSV and did not react by IPMA with PRRSV-infected cell cultures. Sera from rabbits inoculated with BPO3-P failed to neutralize both the European (Lelystad) and American ( ATCC VR-2332) reference isolates of PRRSV and did not react by IPMA with PRRSV-infected cell cultures. Two of the rabbit antipeptide sera were reproducibly reactive by western immunoblot with a diffuse (40 to 45 kDa) band of antigen found in homogenates of MARC-145 cells infected 16 h previously with the Lelystad isolate (Fig. 4) .	./cache/cord-007476-wu9tuvy9.txt	./txt/cord-007476-wu9tuvy9.txt