id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-029839-jxqi9exm	Wang, Ye	Silence of TGF-β1 gene expression reduces prrsv replication and potentiates immunity of immune cells of tibetan pig	2019-09-26		.txt	text/plain	3964	209	55	Conversely, the mRNA level of PRRSV in shRNA treated Tp-PBMCs dramatically decreased, and there were significant increases of the transcription of immune genes, such as interleukin-2 (IL-2), interleukin-4 (IL-4), interferon-alpha (IFN-α), interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), toll-like receptor 3 (TLR3), toll-like receptor 7 (TLR7), Myeloid differentiation primary response gene (88) (MyD88), and interleukin-27p28 (IL-27p28). Therefore the knockdown of TGF-β1 gene expression by shRNA not only inhibits the replication of PRRSV but also improves immune responsiveness following viral infection, suggesting a novel way to facilitate the control of PRRSV infection in pigs. As Table 3 The relative mRNA levels of immune genes in Tp-PBMCs transfected with shTFGβ1-1 and pNeg plasmid and cultured for 48 h. Our experiment firstly screened out effective specific shRNA targeted to pig TGF-β1 gene, it could significantly knock down the mRNA level of TGF-β1 gene and obviously increase the viability of PRRSV infected cells.	./cache/cord-029839-jxqi9exm.txt	./txt/cord-029839-jxqi9exm.txt