id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-282113-sed5xyte	Zheng, Hao	Baculovirus expression of cloned porcine arterivirus generates infectious particles in both insect and mammalian cells	2010-10-15		.txt	text/plain	4553	243	55	In this paper, we have used porcine reproductive and respiratory syndrome virus (PRRSV) as a model virus to address whether infectious mammalian virus particles can be generated in insect cells from recombinant baculovirus. To investigate whether PRRSV particles were generated following infection of insect cells with the recombinant baculovirus, sf9 cells were infected with AcAPRRS and supernatants were harvested 7 days later. We report that infection of insect sf9 cells with the recombinant baculovirus AcAPRRS led to the synthesis of PRRSV proteins and the culture supernatant contained particles that were morphologically indistinguishable from PRRSV virions. In this study, we have demonstrated that a recombinant baculovirus carrying PRRSV genomic cDNA was able to generate infectious PRRSV following transduction of mammalian cells that are themselves refractory to infection with PRRSV in vitro. When the recombinant baculovirus was inoculated onto insect sf9 cells or mammalian Vero and BHK-21 cells, infectious PRRSV virions were generated.	./cache/cord-282113-sed5xyte.txt	./txt/cord-282113-sed5xyte.txt