id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-309428-qkjjxr6p	Li, Liwei	Host miR-26a suppresses replication of porcine reproductive and respiratory syndrome virus by upregulating type I interferons	2015-01-02		.txt	text/plain	5084	324	53	MicroRNAs (miRNAs) play important roles in viral infections, especially by modulating the expression of cellular factors essential to viral replication or the host innate immune response to infection. To identify host miRNAs important to controlling porcine reproductive and respiratory syndrome virus (PRRSV) infection, we screened 15 miRNAs that were previously implicated in innate immunity or antiviral functions. Luciferase reporters showed that miR-26a does not target the PRRSV genome directly but instead affects the expression of type I interferon and the IFN-stimulated genes MX1 and ISG15 during PRRSV infection. We found that miR-26a does not target the PRRSV genome directly, but rather affects the expression of type I interferon and the IFN-stimulated genes MX1 and ISG15 during PRRSV infection. Indirect immunofluorescence assays (IFA) were performed as described previously (Tian et al., 2011) for the detection of nucleocapsid (N) protein in PRRSV vJX143 infected MARC-145 cells or PAMs pre-transfected with miR-26 family or mutant mimics.	./cache/cord-309428-qkjjxr6p.txt	./txt/cord-309428-qkjjxr6p.txt