id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-310771-tnwfp1je	Revilla-Fernández, Sandra	The use of endogenous and exogenous reference RNAs for qualitative and quantitative detection of PRRSV in porcine semen	2005-02-23		.txt	text/plain	5933	297	51	A method was developed for qualitative and quantitative detection of the seminal cell-associated PRRSV RNA in relation to endogenous and exogenous reference RNAs. As endogenous control for one-step real-time reverse transcription (RT)-PCR UBE2D2 mRNA was selected. Particularly for the analysis of persistent infections associated with low copy numbers of PRRSV RNA, UBE2D2 mRNA is an ideal control due to its low expression in seminal cells and its detection in all samples analysed (n = 36). For the development of one-step real-time RT-PCR for four endogenous reference RNAs (HPRT, UBE2D2, PPIA, and HMBS) appropriate target regions were selected and the assay conditions were optimised for amplification efficiency. One-step real-time RT-PCR assays for PRRSV-1 and -2 RNA allowed quantitation with optimal efficiency (Fig. 1a ; standard curves for two additional viremic pigs infected with PRRSV-1 (data not shown)) as achieved for endogenous and Fig. 1 .	./cache/cord-310771-tnwfp1je.txt	./txt/cord-310771-tnwfp1je.txt