id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-319779-n5w1f0rr	Lee, Sang-Myeong	Porcine reproductive and respiratory syndrome virus field isolates differ in in vitro interferon phenotypes	2004-12-08		.txt	text/plain	8214	447	51	North American porcine reproductive and respiratory syndrome virus (PRRSV) field isolates were studied with regard to IFN-α sensitivity and induction in order to understand the role of type I IFN in PRRSV pathogenesis. PRRSV isolates were differentially sensitive to porcine recombinant IFN-α (rIFN-α) and varied in their ability to induce IFN-α in porcine alveolar macrophages (PAM) cultures as measured by a porcine IFN-α specific ELISA on cell culture supernatants. In this study, North American field isolates of PRRSV were evaluated with respect to induction, sensitivity and suppression of an in vitro type I IFN response. UV-inactivated PRRSV stocks were tested to determine whether virus binding to the cells is responsible for enhanced IFN-a production by polyI:C in cells infected with isolate 3 (Fig. 6) . In this study, North American PRRSV field isolates were evaluated with respect to induction, sensitivity and suppression of an in vitro IFN response in PAM cultures. Immune responses in pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV)	./cache/cord-319779-n5w1f0rr.txt	./txt/cord-319779-n5w1f0rr.txt