id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-352967-y1fyke9u	Jiang, Yunbo	N-acetylpenicillamine inhibits the replication of porcine reproductive and respiratory syndrome virus in vitro	2010-07-31		.txt	text/plain	3271	193	49	In this study, we investigated the inhibitory effect of NO on the infection by porcine reproductive and respiratory syndrome virus (PRRSV) in vitro and the role of NO in the defense against PRRSV. We found that the maximal inhibition effect of NAP on PRRSV replication was achieved upon treatment 1 h after virus infection and the virus yield was reduced by approximately 50 fold in the presence of 400 μM NAP. To date, NAP is only used as the negative control for SNAP to study the inhibitory effect of NO on virus replication in previous studies, and its antiviral activity was not reported. Unexpectedly, NAP, the "commonly used" negative control for NO-donor SNAP had a significant antiviral activity against PRRSV replication (Fig. 1a ) at low infectious dose (MOI=0.05). The inhibitory effect of NAP in Marc-145 cells was investigated by determining the kinetics of PRRSV production under different NAP treatments.	./cache/cord-352967-y1fyke9u.txt	./txt/cord-352967-y1fyke9u.txt