id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-015235-lv8mll28	Kim, Hyun	Functional analysis of the receptor binding domain of SARS coronavirus S1 region and its monoclonal antibody	2014-04-16		.txt	text/plain	5855	323	60	The receptor-binding domain (RBD) positioned in S1 can specifically bind to angiotensin-converting enzyme 2 (ACE2) on target cells, and ACE2 regulates the balance between vasoconstrictors and vasodilators within the heart and kidneys. Infection of SARS-CoV is initiated by binding of the S protein to the angiotensin-converting enzyme 2 (ACE2) functional receptor expressed on target cells (Li et al. Our cellular enzyme-linked immunosorbent assay (ELISA) and competitive binding assay using a polyclonal ACE2 antibody indicated that our prepared recombinant RBD fusion protein binds to various tissues as well as NIH3T3 and HEK293 cells through ACE2. After washing with PBS, the RBD fusion protein was incubated for 1 h at room temperature to bind with the ACE2 molecules on the cell membranes. Next, we examined whether RBD binding was blocked by the ACE2 antibody in a Western blot using mouse tissue cell lysates with a pair of membranes.	./cache/cord-015235-lv8mll28.txt	./txt/cord-015235-lv8mll28.txt