id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-332855-u0amf1oh	Parsons, Lisa M.	Glycosylation of the viral attachment protein of avian coronavirus is essential for host cell and receptor binding	2019-03-22		.txt	text/plain	6964	417	59	In silico docking experiments with the recently published cryo-EM structure of the M41 IBV spike protein and our glycosylation results revealed a potential ligand receptor site that is ringed by four glycosylation sites that dramatically impact ligand binding. However, the Beaudette strain is a cell culture-adapted strain, is nonvirulent in chickens (15) , and does not bind chicken tissues known to be important for infectivity (11) , making it difficult to extrapolate these results to clinically relevant IBVs. To characterize and assess the role that glycosylation plays when interacting with host tissues through the RBD of pathogenic IBV strain M41, we used a combination of molecular and analytical techniques, including histochemistry, ELISA, circular dichroism (CD), MS, and docking analyses as listed in Table 1 . Six of the 10 glycosylation sites in the RBD domain of IBV M41 were essential for binding to chicken trachea tissue and an ELISA-presented sialylated oligosaccharide ligand.	./cache/cord-332855-u0amf1oh.txt	./txt/cord-332855-u0amf1oh.txt