id	sid	tid	token	lemma	pos
cord-311035-s3tkbh9r	1	1	key	key	JJ
cord-311035-s3tkbh9r	2	1	:	:	:
cord-311035-s3tkbh9r	2	2	cord-311035-s3tkbh9r	cord-311035-s3tkbh9r	NNP
cord-311035-s3tkbh9r	2	3	authors	author	NNS
cord-311035-s3tkbh9r	2	4	:	:	:
cord-311035-s3tkbh9r	2	5	Procko	Procko	NNP
cord-311035-s3tkbh9r	2	6	,	,	,
cord-311035-s3tkbh9r	2	7	Erik	Erik	NNP
cord-311035-s3tkbh9r	2	8	title	title	NN
cord-311035-s3tkbh9r	2	9	:	:	:
cord-311035-s3tkbh9r	3	1	Deep	deep	JJ
cord-311035-s3tkbh9r	3	2	mutagenesis	mutagenesis	NN
cord-311035-s3tkbh9r	3	3	in	in	IN
cord-311035-s3tkbh9r	3	4	the	the	DT
cord-311035-s3tkbh9r	3	5	study	study	NN
cord-311035-s3tkbh9r	3	6	of	of	IN
cord-311035-s3tkbh9r	3	7	COVID-19	COVID-19	NNP
cord-311035-s3tkbh9r	3	8	:	:	:
cord-311035-s3tkbh9r	3	9	a	a	DT
cord-311035-s3tkbh9r	3	10	technical	technical	JJ
cord-311035-s3tkbh9r	3	11	overview	overview	NN
cord-311035-s3tkbh9r	3	12	for	for	IN
cord-311035-s3tkbh9r	3	13	the	the	DT
cord-311035-s3tkbh9r	3	14	proteomics	proteomic	NNS
cord-311035-s3tkbh9r	3	15	community	community	NN
cord-311035-s3tkbh9r	3	16	date	date	NN
cord-311035-s3tkbh9r	3	17	:	:	:
cord-311035-s3tkbh9r	3	18	2020	2020	CD
cord-311035-s3tkbh9r	3	19	-	-	SYM
cord-311035-s3tkbh9r	3	20	10	10	CD
cord-311035-s3tkbh9r	3	21	-	-	SYM
cord-311035-s3tkbh9r	3	22	21	21	CD
cord-311035-s3tkbh9r	3	23	journal	journal	NN
cord-311035-s3tkbh9r	3	24	:	:	:
cord-311035-s3tkbh9r	4	1	Expert	expert	NN
cord-311035-s3tkbh9r	4	2	review	review	NN
cord-311035-s3tkbh9r	4	3	of	of	IN
cord-311035-s3tkbh9r	4	4	proteomics	proteomic	NNS
cord-311035-s3tkbh9r	4	5	DOI	doi	NN
cord-311035-s3tkbh9r	4	6	:	:	:
cord-311035-s3tkbh9r	4	7	10.1080/14789450.2020.1833721	10.1080/14789450.2020.1833721	NNP
cord-311035-s3tkbh9r	4	8	sha	sha	NNP
cord-311035-s3tkbh9r	4	9	:	:	:
cord-311035-s3tkbh9r	4	10	1255be96610b4b0bbf06bf283e93e5371df0f474	1255be96610b4b0bbf06bf283e93e5371df0f474	NNP
cord-311035-s3tkbh9r	4	11	doc_id	doc_id	CD
cord-311035-s3tkbh9r	4	12	:	:	:
cord-311035-s3tkbh9r	5	1	311035	311035	CD
cord-311035-s3tkbh9r	5	2	cord_uid	cord_uid	NNS
cord-311035-s3tkbh9r	5	3	:	:	:
cord-311035-s3tkbh9r	6	1	s3tkbh9r	s3tkbh9r	:
cord-311035-s3tkbh9r	6	2	Introduction	introduction	NN
cord-311035-s3tkbh9r	7	1	The	the	DT
cord-311035-s3tkbh9r	7	2	spike	spike	NN
cord-311035-s3tkbh9r	7	3	(	(	-LRB-
cord-311035-s3tkbh9r	7	4	S	s	NN
cord-311035-s3tkbh9r	7	5	)	)	-RRB-
cord-311035-s3tkbh9r	7	6	of	of	IN
cord-311035-s3tkbh9r	7	7	SARS	SARS	NNP
cord-311035-s3tkbh9r	7	8	coronavirus	coronavirus	NN
cord-311035-s3tkbh9r	7	9	2	2	CD
cord-311035-s3tkbh9r	7	10	(	(	-LRB-
cord-311035-s3tkbh9r	7	11	SARS	SARS	NNP
cord-311035-s3tkbh9r	7	12	-	-	HYPH
cord-311035-s3tkbh9r	7	13	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	7	14	)	)	-RRB-
cord-311035-s3tkbh9r	7	15	engages	engage	VBZ
cord-311035-s3tkbh9r	7	16	angiotensin	angiotensin	NN
cord-311035-s3tkbh9r	7	17	-	-	HYPH
cord-311035-s3tkbh9r	7	18	converting	convert	VBG
cord-311035-s3tkbh9r	7	19	enzyme	enzyme	NN
cord-311035-s3tkbh9r	7	20	2	2	CD
cord-311035-s3tkbh9r	7	21	(	(	-LRB-
cord-311035-s3tkbh9r	7	22	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	7	23	)	)	-RRB-
cord-311035-s3tkbh9r	7	24	on	on	IN
cord-311035-s3tkbh9r	7	25	a	a	DT
cord-311035-s3tkbh9r	7	26	host	host	NN
cord-311035-s3tkbh9r	7	27	cell	cell	NN
cord-311035-s3tkbh9r	7	28	to	to	TO
cord-311035-s3tkbh9r	7	29	trigger	trigger	VB
cord-311035-s3tkbh9r	7	30	viral	viral	JJ
cord-311035-s3tkbh9r	7	31	-	-	HYPH
cord-311035-s3tkbh9r	7	32	cell	cell	NN
cord-311035-s3tkbh9r	7	33	membrane	membrane	NN
cord-311035-s3tkbh9r	7	34	fusion	fusion	NN
cord-311035-s3tkbh9r	7	35	and	and	CC
cord-311035-s3tkbh9r	7	36	infection	infection	NN
cord-311035-s3tkbh9r	7	37	.	.	.
cord-311035-s3tkbh9r	8	1	The	the	DT
cord-311035-s3tkbh9r	8	2	extracellular	extracellular	JJ
cord-311035-s3tkbh9r	8	3	region	region	NN
cord-311035-s3tkbh9r	8	4	of	of	IN
cord-311035-s3tkbh9r	8	5	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	8	6	can	can	MD
cord-311035-s3tkbh9r	8	7	be	be	VB
cord-311035-s3tkbh9r	8	8	administered	administer	VBN
cord-311035-s3tkbh9r	8	9	as	as	IN
cord-311035-s3tkbh9r	8	10	a	a	DT
cord-311035-s3tkbh9r	8	11	soluble	soluble	JJ
cord-311035-s3tkbh9r	8	12	decoy	decoy	NN
cord-311035-s3tkbh9r	8	13	to	to	TO
cord-311035-s3tkbh9r	8	14	compete	compete	VB
cord-311035-s3tkbh9r	8	15	for	for	IN
cord-311035-s3tkbh9r	8	16	binding	bind	VBG
cord-311035-s3tkbh9r	8	17	sites	site	NNS
cord-311035-s3tkbh9r	8	18	on	on	IN
cord-311035-s3tkbh9r	8	19	the	the	DT
cord-311035-s3tkbh9r	8	20	receptor	receptor	NN
cord-311035-s3tkbh9r	8	21	-	-	HYPH
cord-311035-s3tkbh9r	8	22	binding	bind	VBG
cord-311035-s3tkbh9r	8	23	domain	domain	NN
cord-311035-s3tkbh9r	8	24	(	(	-LRB-
cord-311035-s3tkbh9r	8	25	RBD	RBD	NNP
cord-311035-s3tkbh9r	8	26	)	)	-RRB-
cord-311035-s3tkbh9r	8	27	of	of	IN
cord-311035-s3tkbh9r	8	28	S	S	NNP
cord-311035-s3tkbh9r	8	29	,	,	,
cord-311035-s3tkbh9r	8	30	but	but	CC
cord-311035-s3tkbh9r	8	31	it	-PRON-	PRP
cord-311035-s3tkbh9r	8	32	has	have	VBZ
cord-311035-s3tkbh9r	8	33	only	only	RB
cord-311035-s3tkbh9r	8	34	moderate	moderate	JJ
cord-311035-s3tkbh9r	8	35	affinity	affinity	NN
cord-311035-s3tkbh9r	8	36	and	and	CC
cord-311035-s3tkbh9r	8	37	efficacy	efficacy	NN
cord-311035-s3tkbh9r	8	38	.	.	.
cord-311035-s3tkbh9r	9	1	The	the	DT
cord-311035-s3tkbh9r	9	2	RBD	RBD	NNP
cord-311035-s3tkbh9r	9	3	,	,	,
cord-311035-s3tkbh9r	9	4	which	which	WDT
cord-311035-s3tkbh9r	9	5	is	be	VBZ
cord-311035-s3tkbh9r	9	6	targeted	target	VBN
cord-311035-s3tkbh9r	9	7	by	by	IN
cord-311035-s3tkbh9r	9	8	neutralizing	neutralize	VBG
cord-311035-s3tkbh9r	9	9	antibodies	antibody	NNS
cord-311035-s3tkbh9r	9	10	,	,	,
cord-311035-s3tkbh9r	9	11	may	may	MD
cord-311035-s3tkbh9r	9	12	also	also	RB
cord-311035-s3tkbh9r	9	13	change	change	VB
cord-311035-s3tkbh9r	9	14	and	and	CC
cord-311035-s3tkbh9r	9	15	adapt	adapt	VB
cord-311035-s3tkbh9r	9	16	through	through	IN
cord-311035-s3tkbh9r	9	17	mutation	mutation	NN
cord-311035-s3tkbh9r	9	18	as	as	IN
cord-311035-s3tkbh9r	9	19	SARS	SARS	NNP
cord-311035-s3tkbh9r	9	20	-	-	HYPH
cord-311035-s3tkbh9r	9	21	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	9	22	becomes	become	VBZ
cord-311035-s3tkbh9r	9	23	endemic	endemic	JJ
cord-311035-s3tkbh9r	9	24	,	,	,
cord-311035-s3tkbh9r	9	25	posing	pose	VBG
cord-311035-s3tkbh9r	9	26	challenges	challenge	NNS
cord-311035-s3tkbh9r	9	27	for	for	IN
cord-311035-s3tkbh9r	9	28	therapeutic	therapeutic	JJ
cord-311035-s3tkbh9r	9	29	and	and	CC
cord-311035-s3tkbh9r	9	30	vaccine	vaccine	NN
cord-311035-s3tkbh9r	9	31	development	development	NN
cord-311035-s3tkbh9r	9	32	.	.	.
cord-311035-s3tkbh9r	10	1	Areas	area	NNS
cord-311035-s3tkbh9r	10	2	Covered	cover	VBN
cord-311035-s3tkbh9r	10	3	Deep	deep	JJ
cord-311035-s3tkbh9r	10	4	mutagenesis	mutagenesis	NN
cord-311035-s3tkbh9r	10	5	is	be	VBZ
cord-311035-s3tkbh9r	10	6	a	a	DT
cord-311035-s3tkbh9r	10	7	Big	Big	NNP
cord-311035-s3tkbh9r	10	8	Data	data	NN
cord-311035-s3tkbh9r	10	9	approach	approach	NN
cord-311035-s3tkbh9r	10	10	to	to	IN
cord-311035-s3tkbh9r	10	11	characterizing	characterize	VBG
cord-311035-s3tkbh9r	10	12	sequence	sequence	NN
cord-311035-s3tkbh9r	10	13	variants	variant	NNS
cord-311035-s3tkbh9r	10	14	.	.	.
cord-311035-s3tkbh9r	11	1	A	a	DT
cord-311035-s3tkbh9r	11	2	deep	deep	JJ
cord-311035-s3tkbh9r	11	3	mutational	mutational	JJ
cord-311035-s3tkbh9r	11	4	scan	scan	NN
cord-311035-s3tkbh9r	11	5	of	of	IN
cord-311035-s3tkbh9r	11	6	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	11	7	expressed	express	VBD
cord-311035-s3tkbh9r	11	8	on	on	IN
cord-311035-s3tkbh9r	11	9	human	human	JJ
cord-311035-s3tkbh9r	11	10	cells	cell	NNS
cord-311035-s3tkbh9r	11	11	identified	identify	VBN
cord-311035-s3tkbh9r	11	12	mutations	mutation	NNS
cord-311035-s3tkbh9r	11	13	that	that	WDT
cord-311035-s3tkbh9r	11	14	increase	increase	VBP
cord-311035-s3tkbh9r	11	15	S	s	NN
cord-311035-s3tkbh9r	11	16	affinity	affinity	NN
cord-311035-s3tkbh9r	11	17	and	and	CC
cord-311035-s3tkbh9r	11	18	guided	guide	VBD
cord-311035-s3tkbh9r	11	19	the	the	DT
cord-311035-s3tkbh9r	11	20	engineering	engineering	NN
cord-311035-s3tkbh9r	11	21	of	of	IN
cord-311035-s3tkbh9r	11	22	a	a	DT
cord-311035-s3tkbh9r	11	23	potent	potent	JJ
cord-311035-s3tkbh9r	11	24	and	and	CC
cord-311035-s3tkbh9r	11	25	broad	broad	JJ
cord-311035-s3tkbh9r	11	26	soluble	soluble	JJ
cord-311035-s3tkbh9r	11	27	receptor	receptor	NN
cord-311035-s3tkbh9r	11	28	decoy	decoy	NN
cord-311035-s3tkbh9r	11	29	.	.	.
cord-311035-s3tkbh9r	12	1	A	a	DT
cord-311035-s3tkbh9r	12	2	deep	deep	JJ
cord-311035-s3tkbh9r	12	3	mutational	mutational	JJ
cord-311035-s3tkbh9r	12	4	scan	scan	NN
cord-311035-s3tkbh9r	12	5	of	of	IN
cord-311035-s3tkbh9r	12	6	the	the	DT
cord-311035-s3tkbh9r	12	7	RBD	RBD	NNP
cord-311035-s3tkbh9r	12	8	displayed	display	VBD
cord-311035-s3tkbh9r	12	9	on	on	IN
cord-311035-s3tkbh9r	12	10	the	the	DT
cord-311035-s3tkbh9r	12	11	surface	surface	NN
cord-311035-s3tkbh9r	12	12	of	of	IN
cord-311035-s3tkbh9r	12	13	yeast	yeast	NN
cord-311035-s3tkbh9r	12	14	has	have	VBZ
cord-311035-s3tkbh9r	12	15	revealed	reveal	VBN
cord-311035-s3tkbh9r	12	16	residues	residue	NNS
cord-311035-s3tkbh9r	12	17	tolerant	tolerant	JJ
cord-311035-s3tkbh9r	12	18	of	of	IN
cord-311035-s3tkbh9r	12	19	mutational	mutational	JJ
cord-311035-s3tkbh9r	12	20	changes	change	NNS
cord-311035-s3tkbh9r	12	21	that	that	WDT
cord-311035-s3tkbh9r	12	22	may	may	MD
cord-311035-s3tkbh9r	12	23	act	act	VB
cord-311035-s3tkbh9r	12	24	as	as	IN
cord-311035-s3tkbh9r	12	25	a	a	DT
cord-311035-s3tkbh9r	12	26	source	source	NN
cord-311035-s3tkbh9r	12	27	for	for	IN
cord-311035-s3tkbh9r	12	28	drug	drug	NN
cord-311035-s3tkbh9r	12	29	resistance	resistance	NN
cord-311035-s3tkbh9r	12	30	and	and	CC
cord-311035-s3tkbh9r	12	31	antigenic	antigenic	JJ
cord-311035-s3tkbh9r	12	32	drift	drift	NN
cord-311035-s3tkbh9r	12	33	.	.	.
cord-311035-s3tkbh9r	13	1	Expert	Expert	NNP
cord-311035-s3tkbh9r	13	2	Opinion	Opinion	NNP
cord-311035-s3tkbh9r	13	3	Deep	Deep	NNP
cord-311035-s3tkbh9r	13	4	mutagenesis	mutagenesis	NN
cord-311035-s3tkbh9r	13	5	requires	require	VBZ
cord-311035-s3tkbh9r	13	6	a	a	DT
cord-311035-s3tkbh9r	13	7	selection	selection	NN
cord-311035-s3tkbh9r	13	8	of	of	IN
cord-311035-s3tkbh9r	13	9	diverse	diverse	JJ
cord-311035-s3tkbh9r	13	10	sequence	sequence	NN
cord-311035-s3tkbh9r	13	11	variants	variant	NNS
cord-311035-s3tkbh9r	13	12	;	;	:
cord-311035-s3tkbh9r	13	13	an	an	DT
cord-311035-s3tkbh9r	13	14	in	in	FW
cord-311035-s3tkbh9r	13	15	vitro	vitro	FW
cord-311035-s3tkbh9r	13	16	evolution	evolution	NN
cord-311035-s3tkbh9r	13	17	experiment	experiment	NN
cord-311035-s3tkbh9r	13	18	that	that	WDT
cord-311035-s3tkbh9r	13	19	is	be	VBZ
cord-311035-s3tkbh9r	13	20	tracked	track	VBN
cord-311035-s3tkbh9r	13	21	with	with	IN
cord-311035-s3tkbh9r	13	22	next	next	JJ
cord-311035-s3tkbh9r	13	23	-	-	HYPH
cord-311035-s3tkbh9r	13	24	generation	generation	NN
cord-311035-s3tkbh9r	13	25	sequencing	sequencing	NN
cord-311035-s3tkbh9r	13	26	.	.	.
cord-311035-s3tkbh9r	14	1	The	the	DT
cord-311035-s3tkbh9r	14	2	choice	choice	NN
cord-311035-s3tkbh9r	14	3	of	of	IN
cord-311035-s3tkbh9r	14	4	expression	expression	NN
cord-311035-s3tkbh9r	14	5	system	system	NN
cord-311035-s3tkbh9r	14	6	,	,	,
cord-311035-s3tkbh9r	14	7	diversity	diversity	NN
cord-311035-s3tkbh9r	14	8	of	of	IN
cord-311035-s3tkbh9r	14	9	the	the	DT
cord-311035-s3tkbh9r	14	10	variant	variant	JJ
cord-311035-s3tkbh9r	14	11	library	library	NN
cord-311035-s3tkbh9r	14	12	and	and	CC
cord-311035-s3tkbh9r	14	13	selection	selection	NN
cord-311035-s3tkbh9r	14	14	strategy	strategy	NN
cord-311035-s3tkbh9r	14	15	have	have	VBP
cord-311035-s3tkbh9r	14	16	important	important	JJ
cord-311035-s3tkbh9r	14	17	consequences	consequence	NNS
cord-311035-s3tkbh9r	14	18	for	for	IN
cord-311035-s3tkbh9r	14	19	data	data	NN
cord-311035-s3tkbh9r	14	20	quality	quality	NN
cord-311035-s3tkbh9r	14	21	and	and	CC
cord-311035-s3tkbh9r	14	22	interpretation	interpretation	NN
cord-311035-s3tkbh9r	14	23	.	.	.
cord-311035-s3tkbh9r	15	1	Investigations	investigation	NNS
cord-311035-s3tkbh9r	15	2	of	of	IN
cord-311035-s3tkbh9r	15	3	protein	protein	NN
cord-311035-s3tkbh9r	15	4	mutations	mutation	NNS
cord-311035-s3tkbh9r	15	5	have	have	VBP
cord-311035-s3tkbh9r	15	6	classically	classically	RB
cord-311035-s3tkbh9r	15	7	been	be	VBN
cord-311035-s3tkbh9r	15	8	approached	approach	VBN
cord-311035-s3tkbh9r	15	9	by	by	IN
cord-311035-s3tkbh9r	15	10	precision	precision	NN
cord-311035-s3tkbh9r	15	11	targeting	targeting	NN
cord-311035-s3tkbh9r	15	12	,	,	,
cord-311035-s3tkbh9r	15	13	in	in	IN
cord-311035-s3tkbh9r	15	14	which	which	WDT
cord-311035-s3tkbh9r	15	15	a	a	DT
cord-311035-s3tkbh9r	15	16	small	small	JJ
cord-311035-s3tkbh9r	15	17	number	number	NN
cord-311035-s3tkbh9r	15	18	of	of	IN
cord-311035-s3tkbh9r	15	19	mutations	mutation	NNS
cord-311035-s3tkbh9r	15	20	are	be	VBP
cord-311035-s3tkbh9r	15	21	deliberately	deliberately	RB
cord-311035-s3tkbh9r	15	22	introduced	introduce	VBN
cord-311035-s3tkbh9r	15	23	and	and	CC
cord-311035-s3tkbh9r	15	24	tested	test	VBN
cord-311035-s3tkbh9r	15	25	individually	individually	RB
cord-311035-s3tkbh9r	15	26	.	.	.
cord-311035-s3tkbh9r	16	1	This	this	DT
cord-311035-s3tkbh9r	16	2	requires	require	VBZ
cord-311035-s3tkbh9r	16	3	preconceived	preconceived	JJ
cord-311035-s3tkbh9r	16	4	ideas	idea	NNS
cord-311035-s3tkbh9r	16	5	or	or	CC
cord-311035-s3tkbh9r	16	6	hypotheses	hypothesis	NNS
cord-311035-s3tkbh9r	16	7	on	on	IN
cord-311035-s3tkbh9r	16	8	which	which	WDT
cord-311035-s3tkbh9r	16	9	residues	residue	NNS
cord-311035-s3tkbh9r	16	10	and	and	CC
cord-311035-s3tkbh9r	16	11	what	what	WP
cord-311035-s3tkbh9r	16	12	changes	change	VBZ
cord-311035-s3tkbh9r	16	13	to	to	IN
cord-311035-s3tkbh9r	16	14	those	those	DT
cord-311035-s3tkbh9r	16	15	residues	residue	NNS
cord-311035-s3tkbh9r	16	16	might	may	MD
cord-311035-s3tkbh9r	16	17	be	be	VB
cord-311035-s3tkbh9r	16	18	relevant	relevant	JJ
cord-311035-s3tkbh9r	16	19	.	.	.
cord-311035-s3tkbh9r	17	1	When	when	WRB
cord-311035-s3tkbh9r	17	2	the	the	DT
cord-311035-s3tkbh9r	17	3	important	important	JJ
cord-311035-s3tkbh9r	17	4	residues	residue	NNS
cord-311035-s3tkbh9r	17	5	in	in	IN
cord-311035-s3tkbh9r	17	6	a	a	DT
cord-311035-s3tkbh9r	17	7	protein	protein	NN
cord-311035-s3tkbh9r	17	8	sequence	sequence	NN
cord-311035-s3tkbh9r	17	9	are	be	VBP
cord-311035-s3tkbh9r	17	10	unknown	unknown	JJ
cord-311035-s3tkbh9r	17	11	,	,	,
cord-311035-s3tkbh9r	17	12	screens	screen	NNS
cord-311035-s3tkbh9r	17	13	and	and	CC
cord-311035-s3tkbh9r	17	14	selections	selection	NNS
cord-311035-s3tkbh9r	17	15	can	can	MD
cord-311035-s3tkbh9r	17	16	be	be	VB
cord-311035-s3tkbh9r	17	17	used	use	VBN
cord-311035-s3tkbh9r	17	18	instead	instead	RB
cord-311035-s3tkbh9r	17	19	,	,	,
cord-311035-s3tkbh9r	17	20	in	in	IN
cord-311035-s3tkbh9r	17	21	which	which	WDT
cord-311035-s3tkbh9r	17	22	a	a	DT
cord-311035-s3tkbh9r	17	23	library	library	NN
cord-311035-s3tkbh9r	17	24	of	of	IN
cord-311035-s3tkbh9r	17	25	random	random	JJ
cord-311035-s3tkbh9r	17	26	mutations	mutation	NNS
cord-311035-s3tkbh9r	17	27	is	be	VBZ
cord-311035-s3tkbh9r	17	28	in	in	IN
cord-311035-s3tkbh9r	17	29	some	some	DT
cord-311035-s3tkbh9r	17	30	way	way	NN
cord-311035-s3tkbh9r	17	31	sorted	sort	VBN
cord-311035-s3tkbh9r	17	32	to	to	TO
cord-311035-s3tkbh9r	17	33	enrich	enrich	VB
cord-311035-s3tkbh9r	17	34	for	for	IN
cord-311035-s3tkbh9r	17	35	a	a	DT
cord-311035-s3tkbh9r	17	36	small	small	JJ
cord-311035-s3tkbh9r	17	37	number	number	NN
cord-311035-s3tkbh9r	17	38	of	of	IN
cord-311035-s3tkbh9r	17	39	mutants	mutant	NNS
cord-311035-s3tkbh9r	17	40	with	with	IN
cord-311035-s3tkbh9r	17	41	the	the	DT
cord-311035-s3tkbh9r	17	42	intended	intended	JJ
cord-311035-s3tkbh9r	17	43	phenotype	phenotype	NN
cord-311035-s3tkbh9r	17	44	.	.	.
cord-311035-s3tkbh9r	18	1	Both	both	DT
cord-311035-s3tkbh9r	18	2	experiments	experiment	NNS
cord-311035-s3tkbh9r	18	3	are	be	VBP
cord-311035-s3tkbh9r	18	4	limited	limit	VBN
cord-311035-s3tkbh9r	18	5	in	in	IN
cord-311035-s3tkbh9r	18	6	the	the	DT
cord-311035-s3tkbh9r	18	7	scale	scale	NN
cord-311035-s3tkbh9r	18	8	of	of	IN
cord-311035-s3tkbh9r	18	9	information	information	NN
cord-311035-s3tkbh9r	18	10	they	-PRON-	PRP
cord-311035-s3tkbh9r	18	11	provide	provide	VBP
cord-311035-s3tkbh9r	18	12	.	.	.
cord-311035-s3tkbh9r	19	1	Deep	deep	JJ
cord-311035-s3tkbh9r	19	2	mutagenesis	mutagenesis	NN
cord-311035-s3tkbh9r	19	3	or	or	CC
cord-311035-s3tkbh9r	19	4	deep	deep	JJ
cord-311035-s3tkbh9r	19	5	mutational	mutational	JJ
cord-311035-s3tkbh9r	19	6	scanning	scanning	NN
cord-311035-s3tkbh9r	19	7	take	take	VBP
cord-311035-s3tkbh9r	19	8	advantage	advantage	NN
cord-311035-s3tkbh9r	19	9	of	of	IN
cord-311035-s3tkbh9r	19	10	next	next	JJ
cord-311035-s3tkbh9r	19	11	-	-	HYPH
cord-311035-s3tkbh9r	19	12	generation	generation	NN
cord-311035-s3tkbh9r	19	13	sequencing	sequencing	NN
cord-311035-s3tkbh9r	19	14	to	to	TO
cord-311035-s3tkbh9r	19	15	bring	bring	VB
cord-311035-s3tkbh9r	19	16	experimental	experimental	JJ
cord-311035-s3tkbh9r	19	17	protein	protein	NN
cord-311035-s3tkbh9r	19	18	mutagenesis	mutagenesis	NN
cord-311035-s3tkbh9r	19	19	to	to	IN
cord-311035-s3tkbh9r	19	20	the	the	DT
cord-311035-s3tkbh9r	19	21	realm	realm	NN
cord-311035-s3tkbh9r	19	22	of	of	IN
cord-311035-s3tkbh9r	19	23	Big	Big	NNP
cord-311035-s3tkbh9r	19	24	Data	Data	NNP
cord-311035-s3tkbh9r	19	25	[	[	-LRB-
cord-311035-s3tkbh9r	19	26	1	1	CD
cord-311035-s3tkbh9r	19	27	]	]	-RRB-
cord-311035-s3tkbh9r	19	28	.	.	.
cord-311035-s3tkbh9r	20	1	A	a	DT
cord-311035-s3tkbh9r	20	2	screen	screen	NN
cord-311035-s3tkbh9r	20	3	or	or	CC
cord-311035-s3tkbh9r	20	4	selection	selection	NN
cord-311035-s3tkbh9r	20	5	of	of	IN
cord-311035-s3tkbh9r	20	6	a	a	DT
cord-311035-s3tkbh9r	20	7	diverse	diverse	JJ
cord-311035-s3tkbh9r	20	8	library	library	NN
cord-311035-s3tkbh9r	20	9	of	of	IN
cord-311035-s3tkbh9r	20	10	variants	variant	NNS
cord-311035-s3tkbh9r	20	11	is	be	VBZ
cord-311035-s3tkbh9r	20	12	tracked	track	VBN
cord-311035-s3tkbh9r	20	13	by	by	IN
cord-311035-s3tkbh9r	20	14	next	next	JJ
cord-311035-s3tkbh9r	20	15	-	-	HYPH
cord-311035-s3tkbh9r	20	16	generation	generation	NN
cord-311035-s3tkbh9r	20	17	sequencing	sequencing	NN
cord-311035-s3tkbh9r	20	18	to	to	TO
cord-311035-s3tkbh9r	20	19	observe	observe	VB
cord-311035-s3tkbh9r	20	20	how	how	WRB
cord-311035-s3tkbh9r	20	21	the	the	DT
cord-311035-s3tkbh9r	20	22	population	population	NN
cord-311035-s3tkbh9r	20	23	's	's	POS
cord-311035-s3tkbh9r	20	24	genetic	genetic	JJ
cord-311035-s3tkbh9r	20	25	makeup	makeup	NN
cord-311035-s3tkbh9r	20	26	changes	change	NNS
cord-311035-s3tkbh9r	20	27	.	.	.
cord-311035-s3tkbh9r	21	1	Mutations	mutation	NNS
cord-311035-s3tkbh9r	21	2	with	with	IN
cord-311035-s3tkbh9r	21	3	enhanced	enhanced	JJ
cord-311035-s3tkbh9r	21	4	function	function	NN
cord-311035-s3tkbh9r	21	5	are	be	VBP
cord-311035-s3tkbh9r	21	6	enriched	enrich	VBN
cord-311035-s3tkbh9r	21	7	,	,	,
cord-311035-s3tkbh9r	21	8	while	while	IN
cord-311035-s3tkbh9r	21	9	deleterious	deleterious	JJ
cord-311035-s3tkbh9r	21	10	mutations	mutation	NNS
cord-311035-s3tkbh9r	21	11	are	be	VBP
cord-311035-s3tkbh9r	21	12	depleted	deplete	VBN
cord-311035-s3tkbh9r	21	13	;	;	:
cord-311035-s3tkbh9r	21	14	the	the	DT
cord-311035-s3tkbh9r	21	15	enrichment	enrichment	NN
cord-311035-s3tkbh9r	21	16	ratio	ratio	NN
cord-311035-s3tkbh9r	21	17	comparing	compare	VBG
cord-311035-s3tkbh9r	21	18	frequencies	frequency	NNS
cord-311035-s3tkbh9r	21	19	in	in	IN
cord-311035-s3tkbh9r	21	20	the	the	DT
cord-311035-s3tkbh9r	21	21	selected	select	VBN
cord-311035-s3tkbh9r	21	22	population	population	NN
cord-311035-s3tkbh9r	21	23	with	with	IN
cord-311035-s3tkbh9r	21	24	the	the	DT
cord-311035-s3tkbh9r	21	25	naive	naive	JJ
cord-311035-s3tkbh9r	21	26	library	library	NN
cord-311035-s3tkbh9r	21	27	thus	thus	RB
cord-311035-s3tkbh9r	21	28	acts	act	VBZ
cord-311035-s3tkbh9r	21	29	as	as	IN
cord-311035-s3tkbh9r	21	30	a	a	DT
cord-311035-s3tkbh9r	21	31	proxy	proxy	NN
cord-311035-s3tkbh9r	21	32	for	for	IN
cord-311035-s3tkbh9r	21	33	relative	relative	JJ
cord-311035-s3tkbh9r	21	34	phenotype	phenotype	NN
cord-311035-s3tkbh9r	21	35	.	.	.
cord-311035-s3tkbh9r	22	1	Now	now	RB
cord-311035-s3tkbh9r	22	2	,	,	,
cord-311035-s3tkbh9r	22	3	the	the	DT
cord-311035-s3tkbh9r	22	4	relative	relative	JJ
cord-311035-s3tkbh9r	22	5	effects	effect	NNS
cord-311035-s3tkbh9r	22	6	of	of	IN
cord-311035-s3tkbh9r	22	7	thousands	thousand	NNS
cord-311035-s3tkbh9r	22	8	of	of	IN
cord-311035-s3tkbh9r	22	9	mutations	mutation	NNS
cord-311035-s3tkbh9r	22	10	can	can	MD
cord-311035-s3tkbh9r	22	11	be	be	VB
cord-311035-s3tkbh9r	22	12	assessed	assess	VBN
cord-311035-s3tkbh9r	22	13	simultaneously	simultaneously	RB
cord-311035-s3tkbh9r	22	14	in	in	IN
cord-311035-s3tkbh9r	22	15	a	a	DT
cord-311035-s3tkbh9r	22	16	single	single	JJ
cord-311035-s3tkbh9r	22	17	experiment	experiment	NN
cord-311035-s3tkbh9r	22	18	and	and	CC
cord-311035-s3tkbh9r	22	19	a	a	DT
cord-311035-s3tkbh9r	22	20	comprehensive	comprehensive	JJ
cord-311035-s3tkbh9r	22	21	mutational	mutational	JJ
cord-311035-s3tkbh9r	22	22	landscape	landscape	NN
cord-311035-s3tkbh9r	22	23	can	can	MD
cord-311035-s3tkbh9r	22	24	be	be	VB
cord-311035-s3tkbh9r	22	25	calculated	calculate	VBN
cord-311035-s3tkbh9r	22	26	from	from	IN
cord-311035-s3tkbh9r	22	27	experimental	experimental	JJ
cord-311035-s3tkbh9r	22	28	data	datum	NNS
cord-311035-s3tkbh9r	22	29	.	.	.
cord-311035-s3tkbh9r	23	1	Deep	deep	JJ
cord-311035-s3tkbh9r	23	2	mutagenesis	mutagenesis	NN
cord-311035-s3tkbh9r	23	3	has	have	VBZ
cord-311035-s3tkbh9r	23	4	been	be	VBN
cord-311035-s3tkbh9r	23	5	developed	develop	VBN
cord-311035-s3tkbh9r	23	6	by	by	IN
cord-311035-s3tkbh9r	23	7	multiple	multiple	JJ
cord-311035-s3tkbh9r	23	8	groups	group	NNS
cord-311035-s3tkbh9r	23	9	over	over	IN
cord-311035-s3tkbh9r	23	10	the	the	DT
cord-311035-s3tkbh9r	23	11	past	past	JJ
cord-311035-s3tkbh9r	23	12	decade	decade	NN
cord-311035-s3tkbh9r	23	13	[	[	-LRB-
cord-311035-s3tkbh9r	23	14	2	2	CD
cord-311035-s3tkbh9r	23	15	]	]	-RRB-
cord-311035-s3tkbh9r	23	16	[	[	-LRB-
cord-311035-s3tkbh9r	23	17	3	3	CD
cord-311035-s3tkbh9r	23	18	]	]	-RRB-
cord-311035-s3tkbh9r	23	19	[	[	-LRB-
cord-311035-s3tkbh9r	23	20	4	4	CD
cord-311035-s3tkbh9r	23	21	]	]	-RRB-
cord-311035-s3tkbh9r	23	22	[	[	-LRB-
cord-311035-s3tkbh9r	23	23	5	5	CD
cord-311035-s3tkbh9r	23	24	]	]	-RRB-
cord-311035-s3tkbh9r	23	25	[	[	-LRB-
cord-311035-s3tkbh9r	23	26	6	6	CD
cord-311035-s3tkbh9r	23	27	]	]	-RRB-
cord-311035-s3tkbh9r	23	28	[	[	-LRB-
cord-311035-s3tkbh9r	23	29	7	7	CD
cord-311035-s3tkbh9r	23	30	]	]	-RRB-
cord-311035-s3tkbh9r	24	1	[	[	-LRB-
cord-311035-s3tkbh9r	24	2	8	8	CD
cord-311035-s3tkbh9r	24	3	]	]	-RRB-
cord-311035-s3tkbh9r	24	4	[	[	-LRB-
cord-311035-s3tkbh9r	24	5	9	9	CD
cord-311035-s3tkbh9r	24	6	]	]	-RRB-
cord-311035-s3tkbh9r	24	7	[	[	-LRB-
cord-311035-s3tkbh9r	24	8	10	10	CD
cord-311035-s3tkbh9r	24	9	]	]	-RRB-
cord-311035-s3tkbh9r	24	10	[	[	-LRB-
cord-311035-s3tkbh9r	24	11	11	11	CD
cord-311035-s3tkbh9r	24	12	]	]	-RRB-
cord-311035-s3tkbh9r	25	1	[	[	-LRB-
cord-311035-s3tkbh9r	25	2	12	12	CD
cord-311035-s3tkbh9r	25	3	]	]	-RRB-
cord-311035-s3tkbh9r	25	4	[	[	-LRB-
cord-311035-s3tkbh9r	25	5	13	13	CD
cord-311035-s3tkbh9r	25	6	]	]	-RRB-
cord-311035-s3tkbh9r	25	7	and	and	CC
cord-311035-s3tkbh9r	25	8	has	have	VBZ
cord-311035-s3tkbh9r	25	9	proven	prove	VBN
cord-311035-s3tkbh9r	25	10	especially	especially	RB
cord-311035-s3tkbh9r	25	11	invaluable	invaluable	JJ
cord-311035-s3tkbh9r	25	12	to	to	TO
cord-311035-s3tkbh9r	25	13	meet	meet	VB
cord-311035-s3tkbh9r	25	14	three	three	CD
cord-311035-s3tkbh9r	25	15	goals	goal	NNS
cord-311035-s3tkbh9r	25	16	:	:	:
cord-311035-s3tkbh9r	25	17	assisting	assist	VBG
cord-311035-s3tkbh9r	25	18	protein	protein	NN
cord-311035-s3tkbh9r	25	19	engineering	engineering	NN
cord-311035-s3tkbh9r	25	20	,	,	,
cord-311035-s3tkbh9r	25	21	understanding	understand	VBG
cord-311035-s3tkbh9r	25	22	mutational	mutational	JJ
cord-311035-s3tkbh9r	25	23	tolerance	tolerance	NN
cord-311035-s3tkbh9r	25	24	within	within	IN
cord-311035-s3tkbh9r	25	25	a	a	DT
cord-311035-s3tkbh9r	25	26	protein	protein	NN
cord-311035-s3tkbh9r	25	27	sequence	sequence	NN
cord-311035-s3tkbh9r	25	28	,	,	,
cord-311035-s3tkbh9r	25	29	and	and	CC
cord-311035-s3tkbh9r	25	30	predicting	predict	VBG
cord-311035-s3tkbh9r	25	31	which	which	WDT
cord-311035-s3tkbh9r	25	32	mutations	mutation	NNS
cord-311035-s3tkbh9r	25	33	might	may	MD
cord-311035-s3tkbh9r	25	34	be	be	VB
cord-311035-s3tkbh9r	25	35	associated	associate	VBN
cord-311035-s3tkbh9r	25	36	with	with	IN
cord-311035-s3tkbh9r	25	37	adverse	adverse	JJ
cord-311035-s3tkbh9r	25	38	disease	disease	NN
cord-311035-s3tkbh9r	25	39	outcomes	outcome	NNS
cord-311035-s3tkbh9r	25	40	,	,	,
cord-311035-s3tkbh9r	25	41	especially	especially	RB
cord-311035-s3tkbh9r	25	42	in	in	IN
cord-311035-s3tkbh9r	25	43	the	the	DT
cord-311035-s3tkbh9r	25	44	context	context	NN
cord-311035-s3tkbh9r	25	45	of	of	IN
cord-311035-s3tkbh9r	25	46	cancer	cancer	NN
cord-311035-s3tkbh9r	25	47	or	or	CC
cord-311035-s3tkbh9r	25	48	drug	drug	NN
cord-311035-s3tkbh9r	25	49	resistance	resistance	NN
cord-311035-s3tkbh9r	25	50	.	.	.
cord-311035-s3tkbh9r	26	1	Two	two	CD
cord-311035-s3tkbh9r	26	2	recent	recent	JJ
cord-311035-s3tkbh9r	26	3	and	and	CC
cord-311035-s3tkbh9r	26	4	prominent	prominent	JJ
cord-311035-s3tkbh9r	26	5	studies	study	NNS
cord-311035-s3tkbh9r	26	6	of	of	IN
cord-311035-s3tkbh9r	26	7	SARS	SARS	NNP
cord-311035-s3tkbh9r	26	8	coronavirus	coronavirus	NN
cord-311035-s3tkbh9r	26	9	2	2	CD
cord-311035-s3tkbh9r	27	1	(	(	-LRB-
cord-311035-s3tkbh9r	27	2	SARS	SARS	NNP
cord-311035-s3tkbh9r	27	3	-	-	HYPH
cord-311035-s3tkbh9r	27	4	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	27	5	)	)	-RRB-
cord-311035-s3tkbh9r	27	6	have	have	VBP
cord-311035-s3tkbh9r	27	7	used	use	VBN
cord-311035-s3tkbh9r	27	8	deep	deep	JJ
cord-311035-s3tkbh9r	27	9	mutagenesis	mutagenesis	NN
cord-311035-s3tkbh9r	27	10	to	to	TO
cord-311035-s3tkbh9r	27	11	address	address	VB
cord-311035-s3tkbh9r	27	12	each	each	DT
cord-311035-s3tkbh9r	27	13	of	of	IN
cord-311035-s3tkbh9r	27	14	these	these	DT
cord-311035-s3tkbh9r	27	15	problems	problem	NNS
cord-311035-s3tkbh9r	27	16	[	[	-LRB-
cord-311035-s3tkbh9r	27	17	14	14	CD
cord-311035-s3tkbh9r	27	18	,	,	,
cord-311035-s3tkbh9r	27	19	15	15	CD
cord-311035-s3tkbh9r	27	20	]	]	-RRB-
cord-311035-s3tkbh9r	27	21	.	.	.
cord-311035-s3tkbh9r	28	1	This	this	DT
cord-311035-s3tkbh9r	28	2	Special	Special	NNP
cord-311035-s3tkbh9r	28	3	Report	Report	NNP
cord-311035-s3tkbh9r	28	4	summarizes	summarize	VBZ
cord-311035-s3tkbh9r	28	5	the	the	DT
cord-311035-s3tkbh9r	28	6	two	two	CD
cord-311035-s3tkbh9r	28	7	studies	study	NNS
cord-311035-s3tkbh9r	28	8	with	with	IN
cord-311035-s3tkbh9r	28	9	a	a	DT
cord-311035-s3tkbh9r	28	10	focus	focus	NN
cord-311035-s3tkbh9r	28	11	on	on	IN
cord-311035-s3tkbh9r	28	12	experimental	experimental	JJ
cord-311035-s3tkbh9r	28	13	details	detail	NNS
cord-311035-s3tkbh9r	28	14	and	and	CC
cord-311035-s3tkbh9r	28	15	caveats	caveat	NNS
cord-311035-s3tkbh9r	28	16	that	that	WDT
cord-311035-s3tkbh9r	28	17	will	will	MD
cord-311035-s3tkbh9r	28	18	be	be	VB
cord-311035-s3tkbh9r	28	19	unfamiliar	unfamiliar	JJ
cord-311035-s3tkbh9r	28	20	to	to	IN
cord-311035-s3tkbh9r	28	21	those	those	DT
cord-311035-s3tkbh9r	28	22	outside	outside	IN
cord-311035-s3tkbh9r	28	23	the	the	DT
cord-311035-s3tkbh9r	28	24	deep	deep	JJ
cord-311035-s3tkbh9r	28	25	mutational	mutational	JJ
cord-311035-s3tkbh9r	28	26	scanning	scanning	NN
cord-311035-s3tkbh9r	28	27	community	community	NN
cord-311035-s3tkbh9r	28	28	.	.	.
cord-311035-s3tkbh9r	29	1	Two	two	CD
cord-311035-s3tkbh9r	29	2	deep	deep	JJ
cord-311035-s3tkbh9r	29	3	mutagenesis	mutagenesis	NN
cord-311035-s3tkbh9r	29	4	studies	study	NNS
cord-311035-s3tkbh9r	29	5	have	have	VBP
cord-311035-s3tkbh9r	29	6	determined	determine	VBN
cord-311035-s3tkbh9r	29	7	how	how	WRB
cord-311035-s3tkbh9r	29	8	thousands	thousand	NNS
cord-311035-s3tkbh9r	29	9	of	of	IN
cord-311035-s3tkbh9r	29	10	mutations	mutation	NNS
cord-311035-s3tkbh9r	29	11	within	within	IN
cord-311035-s3tkbh9r	29	12	the	the	DT
cord-311035-s3tkbh9r	29	13	SARS	SARS	NNP
cord-311035-s3tkbh9r	29	14	-	-	HYPH
cord-311035-s3tkbh9r	29	15	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	29	16	spike	spike	NN
cord-311035-s3tkbh9r	29	17	or	or	CC
cord-311035-s3tkbh9r	29	18	the	the	DT
cord-311035-s3tkbh9r	29	19	virus	virus	NN
cord-311035-s3tkbh9r	29	20	'	'	POS
cord-311035-s3tkbh9r	29	21	human	human	JJ
cord-311035-s3tkbh9r	29	22	receptor	receptor	NN
cord-311035-s3tkbh9r	29	23	affect	affect	VBP
cord-311035-s3tkbh9r	29	24	their	-PRON-	PRP$
cord-311035-s3tkbh9r	29	25	binding	binding	NN
cord-311035-s3tkbh9r	29	26	.	.	.
cord-311035-s3tkbh9r	30	1	The	the	DT
cord-311035-s3tkbh9r	30	2	data	datum	NNS
cord-311035-s3tkbh9r	30	3	have	have	VBP
cord-311035-s3tkbh9r	30	4	proven	prove	VBN
cord-311035-s3tkbh9r	30	5	invaluable	invaluable	JJ
cord-311035-s3tkbh9r	30	6	for	for	IN
cord-311035-s3tkbh9r	30	7	engineering	engineer	VBG
cord-311035-s3tkbh9r	30	8	high	high	JJ
cord-311035-s3tkbh9r	30	9	affinity	affinity	NN
cord-311035-s3tkbh9r	30	10	decoy	decoy	NN
cord-311035-s3tkbh9r	30	11	receptors	receptor	NNS
cord-311035-s3tkbh9r	30	12	that	that	WDT
cord-311035-s3tkbh9r	30	13	are	be	VBP
cord-311035-s3tkbh9r	30	14	under	under	IN
cord-311035-s3tkbh9r	30	15	preclinical	preclinical	JJ
cord-311035-s3tkbh9r	30	16	development	development	NN
cord-311035-s3tkbh9r	30	17	as	as	IN
cord-311035-s3tkbh9r	30	18	a	a	DT
cord-311035-s3tkbh9r	30	19	COVID-19	COVID-19	NNP
cord-311035-s3tkbh9r	30	20	therapy	therapy	NN
cord-311035-s3tkbh9r	30	21	,	,	,
cord-311035-s3tkbh9r	30	22	and	and	CC
cord-311035-s3tkbh9r	30	23	have	have	VBP
cord-311035-s3tkbh9r	30	24	revealed	reveal	VBN
cord-311035-s3tkbh9r	30	25	the	the	DT
cord-311035-s3tkbh9r	30	26	scope	scope	NN
cord-311035-s3tkbh9r	30	27	of	of	IN
cord-311035-s3tkbh9r	30	28	mutational	mutational	JJ
cord-311035-s3tkbh9r	30	29	tolerance	tolerance	NN
cord-311035-s3tkbh9r	30	30	within	within	IN
cord-311035-s3tkbh9r	30	31	the	the	DT
cord-311035-s3tkbh9r	30	32	spike	spike	NN
cord-311035-s3tkbh9r	30	33	that	that	WDT
cord-311035-s3tkbh9r	30	34	may	may	MD
cord-311035-s3tkbh9r	30	35	have	have	VB
cord-311035-s3tkbh9r	30	36	bearing	bear	VBG
cord-311035-s3tkbh9r	30	37	on	on	IN
cord-311035-s3tkbh9r	30	38	genetic	genetic	JJ
cord-311035-s3tkbh9r	30	39	drift	drift	NN
cord-311035-s3tkbh9r	30	40	as	as	IN
cord-311035-s3tkbh9r	30	41	the	the	DT
cord-311035-s3tkbh9r	30	42	virus	virus	NN
cord-311035-s3tkbh9r	30	43	becomes	become	VBZ
cord-311035-s3tkbh9r	30	44	endemic	endemic	JJ
cord-311035-s3tkbh9r	30	45	and	and	CC
cord-311035-s3tkbh9r	30	46	changes	change	NNS
cord-311035-s3tkbh9r	30	47	over	over	IN
cord-311035-s3tkbh9r	30	48	time	time	NN
cord-311035-s3tkbh9r	30	49	.	.	.
cord-311035-s3tkbh9r	31	1	While	while	IN
cord-311035-s3tkbh9r	31	2	these	these	DT
cord-311035-s3tkbh9r	31	3	two	two	CD
cord-311035-s3tkbh9r	31	4	studies	study	NNS
cord-311035-s3tkbh9r	31	5	focused	focus	VBD
cord-311035-s3tkbh9r	31	6	on	on	IN
cord-311035-s3tkbh9r	31	7	expression	expression	NN
cord-311035-s3tkbh9r	31	8	and	and	CC
cord-311035-s3tkbh9r	31	9	binding	binding	NN
cord-311035-s3tkbh9r	31	10	between	between	IN
cord-311035-s3tkbh9r	31	11	the	the	DT
cord-311035-s3tkbh9r	31	12	viral	viral	JJ
cord-311035-s3tkbh9r	31	13	spike	spike	NN
cord-311035-s3tkbh9r	31	14	and	and	CC
cord-311035-s3tkbh9r	31	15	its	-PRON-	PRP$
cord-311035-s3tkbh9r	31	16	receptor	receptor	NN
cord-311035-s3tkbh9r	31	17	,	,	,
cord-311035-s3tkbh9r	31	18	the	the	DT
cord-311035-s3tkbh9r	31	19	underlying	underlie	VBG
cord-311035-s3tkbh9r	31	20	selection	selection	NN
cord-311035-s3tkbh9r	31	21	strategies	strategy	NNS
cord-311035-s3tkbh9r	31	22	used	use	VBN
cord-311035-s3tkbh9r	31	23	in	in	IN
cord-311035-s3tkbh9r	31	24	deep	deep	JJ
cord-311035-s3tkbh9r	31	25	mutational	mutational	JJ
cord-311035-s3tkbh9r	31	26	scans	scan	NNS
cord-311035-s3tkbh9r	31	27	are	be	VBP
cord-311035-s3tkbh9r	31	28	increasingly	increasingly	RB
cord-311035-s3tkbh9r	31	29	tied	tie	VBN
cord-311035-s3tkbh9r	31	30	to	to	IN
cord-311035-s3tkbh9r	31	31	more	more	JJR
cord-311035-s3tkbh9r	31	32	complex	complex	JJ
cord-311035-s3tkbh9r	31	33	phenotypes	phenotype	NNS
cord-311035-s3tkbh9r	31	34	,	,	,
cord-311035-s3tkbh9r	31	35	such	such	JJ
cord-311035-s3tkbh9r	31	36	as	as	IN
cord-311035-s3tkbh9r	31	37	selections	selection	NNS
cord-311035-s3tkbh9r	31	38	for	for	IN
cord-311035-s3tkbh9r	31	39	structural	structural	JJ
cord-311035-s3tkbh9r	31	40	stability	stability	NN
cord-311035-s3tkbh9r	31	41	based	base	VBN
cord-311035-s3tkbh9r	31	42	on	on	IN
cord-311035-s3tkbh9r	31	43	protease	protease	NN
cord-311035-s3tkbh9r	31	44	-	-	HYPH
cord-311035-s3tkbh9r	31	45	sensitivity	sensitivity	NN
cord-311035-s3tkbh9r	31	46	[	[	-LRB-
cord-311035-s3tkbh9r	31	47	16	16	CD
cord-311035-s3tkbh9r	31	48	]	]	-RRB-
cord-311035-s3tkbh9r	31	49	,	,	,
cord-311035-s3tkbh9r	31	50	using	use	VBG
cord-311035-s3tkbh9r	31	51	competing	compete	VBG
cord-311035-s3tkbh9r	31	52	ligands	ligand	NNS
cord-311035-s3tkbh9r	31	53	to	to	TO
cord-311035-s3tkbh9r	31	54	engineer	engineer	VB
cord-311035-s3tkbh9r	31	55	specificity	specificity	NN
cord-311035-s3tkbh9r	31	56	into	into	IN
cord-311035-s3tkbh9r	31	57	proteins	protein	NNS
cord-311035-s3tkbh9r	31	58	including	include	VBG
cord-311035-s3tkbh9r	31	59	viral	viral	JJ
cord-311035-s3tkbh9r	31	60	receptors	receptor	NNS
cord-311035-s3tkbh9r	31	61	[	[	-LRB-
cord-311035-s3tkbh9r	31	62	17	17	CD
cord-311035-s3tkbh9r	31	63	]	]	-RRB-
cord-311035-s3tkbh9r	31	64	[	[	-LRB-
cord-311035-s3tkbh9r	31	65	18	18	CD
cord-311035-s3tkbh9r	31	66	]	]	-RRB-
cord-311035-s3tkbh9r	31	67	[	[	-LRB-
cord-311035-s3tkbh9r	31	68	19	19	CD
cord-311035-s3tkbh9r	31	69	]	]	-RRB-
cord-311035-s3tkbh9r	31	70	,	,	,
cord-311035-s3tkbh9r	31	71	and	and	CC
cord-311035-s3tkbh9r	31	72	selections	selection	NNS
cord-311035-s3tkbh9r	31	73	based	base	VBN
cord-311035-s3tkbh9r	31	74	on	on	IN
cord-311035-s3tkbh9r	31	75	catalytic	catalytic	JJ
cord-311035-s3tkbh9r	31	76	or	or	CC
cord-311035-s3tkbh9r	31	77	biological	biological	JJ
cord-311035-s3tkbh9r	31	78	activity	activity	NN
cord-311035-s3tkbh9r	31	79	[	[	-LRB-
cord-311035-s3tkbh9r	31	80	20	20	CD
cord-311035-s3tkbh9r	31	81	]	]	-RRB-
cord-311035-s3tkbh9r	31	82	[	[	-LRB-
cord-311035-s3tkbh9r	31	83	21	21	CD
cord-311035-s3tkbh9r	31	84	]	]	-RRB-
cord-311035-s3tkbh9r	32	1	[	[	-LRB-
cord-311035-s3tkbh9r	32	2	22	22	CD
cord-311035-s3tkbh9r	32	3	]	]	-RRB-
cord-311035-s3tkbh9r	32	4	[	[	-LRB-
cord-311035-s3tkbh9r	32	5	23	23	CD
cord-311035-s3tkbh9r	32	6	]	]	-RRB-
cord-311035-s3tkbh9r	32	7	.	.	.
cord-311035-s3tkbh9r	33	1	Undoubtedly	undoubtedly	RB
cord-311035-s3tkbh9r	33	2	there	there	EX
cord-311035-s3tkbh9r	33	3	are	be	VBP
cord-311035-s3tkbh9r	33	4	more	more	JJR
cord-311035-s3tkbh9r	33	5	questions	question	NNS
cord-311035-s3tkbh9r	33	6	related	relate	VBN
cord-311035-s3tkbh9r	33	7	to	to	IN
cord-311035-s3tkbh9r	33	8	SARS	SARS	NNP
cord-311035-s3tkbh9r	33	9	-	-	HYPH
cord-311035-s3tkbh9r	33	10	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	33	11	biology	biology	NN
cord-311035-s3tkbh9r	33	12	and	and	CC
cord-311035-s3tkbh9r	33	13	the	the	DT
cord-311035-s3tkbh9r	33	14	biochemistry	biochemistry	NN
cord-311035-s3tkbh9r	33	15	of	of	IN
cord-311035-s3tkbh9r	33	16	its	-PRON-	PRP$
cord-311035-s3tkbh9r	34	1	While	while	IN
cord-311035-s3tkbh9r	34	2	much	much	JJ
cord-311035-s3tkbh9r	34	3	attention	attention	NN
cord-311035-s3tkbh9r	34	4	has	have	VBZ
cord-311035-s3tkbh9r	34	5	been	be	VBN
cord-311035-s3tkbh9r	34	6	given	give	VBN
cord-311035-s3tkbh9r	34	7	to	to	IN
cord-311035-s3tkbh9r	34	8	isolating	isolate	VBG
cord-311035-s3tkbh9r	34	9	monoclonal	monoclonal	JJ
cord-311035-s3tkbh9r	34	10	antibodies	antibody	NNS
cord-311035-s3tkbh9r	34	11	with	with	IN
cord-311035-s3tkbh9r	34	12	tight	tight	JJ
cord-311035-s3tkbh9r	34	13	affinity	affinity	NN
cord-311035-s3tkbh9r	34	14	for	for	IN
cord-311035-s3tkbh9r	34	15	the	the	DT
cord-311035-s3tkbh9r	34	16	SARS	SARS	NNP
cord-311035-s3tkbh9r	34	17	-	-	HYPH
cord-311035-s3tkbh9r	34	18	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	34	19	spike	spike	NN
cord-311035-s3tkbh9r	34	20	(	(	-LRB-
cord-311035-s3tkbh9r	34	21	S	s	NN
cord-311035-s3tkbh9r	34	22	)	)	-RRB-
cord-311035-s3tkbh9r	34	23	glycoprotein	glycoprotein	NN
cord-311035-s3tkbh9r	34	24	[	[	-LRB-
cord-311035-s3tkbh9r	34	25	24	24	CD
cord-311035-s3tkbh9r	34	26	]	]	-RRB-
cord-311035-s3tkbh9r	34	27	[	[	-LRB-
cord-311035-s3tkbh9r	34	28	25	25	CD
cord-311035-s3tkbh9r	34	29	]	]	-RRB-
cord-311035-s3tkbh9r	34	30	[	[	-LRB-
cord-311035-s3tkbh9r	34	31	26	26	CD
cord-311035-s3tkbh9r	34	32	]	]	-RRB-
cord-311035-s3tkbh9r	34	33	[	[	-LRB-
cord-311035-s3tkbh9r	34	34	27	27	CD
cord-311035-s3tkbh9r	34	35	]	]	-RRB-
cord-311035-s3tkbh9r	34	36	[	[	-LRB-
cord-311035-s3tkbh9r	34	37	28	28	CD
cord-311035-s3tkbh9r	34	38	]	]	-RRB-
cord-311035-s3tkbh9r	34	39	[	[	-LRB-
cord-311035-s3tkbh9r	34	40	29	29	CD
cord-311035-s3tkbh9r	34	41	]	]	-RRB-
cord-311035-s3tkbh9r	34	42	[	[	-LRB-
cord-311035-s3tkbh9r	34	43	30	30	CD
cord-311035-s3tkbh9r	34	44	]	]	-RRB-
cord-311035-s3tkbh9r	34	45	,	,	,
cord-311035-s3tkbh9r	34	46	an	an	DT
cord-311035-s3tkbh9r	34	47	alternative	alternative	NN
cord-311035-s3tkbh9r	34	48	is	be	VBZ
cord-311035-s3tkbh9r	34	49	to	to	TO
cord-311035-s3tkbh9r	34	50	use	use	VB
cord-311035-s3tkbh9r	34	51	the	the	DT
cord-311035-s3tkbh9r	34	52	entry	entry	NN
cord-311035-s3tkbh9r	34	53	receptor	receptor	NN
cord-311035-s3tkbh9r	34	54	as	as	IN
cord-311035-s3tkbh9r	34	55	a	a	DT
cord-311035-s3tkbh9r	34	56	soluble	soluble	JJ
cord-311035-s3tkbh9r	34	57	decoy	decoy	NN
cord-311035-s3tkbh9r	34	58	to	to	TO
cord-311035-s3tkbh9r	34	59	neutralize	neutralize	VB
cord-311035-s3tkbh9r	34	60	infection	infection	NN
cord-311035-s3tkbh9r	34	61	.	.	.
cord-311035-s3tkbh9r	35	1	S	S	NNP
cord-311035-s3tkbh9r	35	2	is	be	VBZ
cord-311035-s3tkbh9r	35	3	a	a	DT
cord-311035-s3tkbh9r	35	4	class	class	NN
cord-311035-s3tkbh9r	36	1	I	-PRON-	PRP
cord-311035-s3tkbh9r	36	2	viral	viral	JJ
cord-311035-s3tkbh9r	36	3	fusion	fusion	NN
cord-311035-s3tkbh9r	36	4	protein	protein	NN
cord-311035-s3tkbh9r	36	5	that	that	WDT
cord-311035-s3tkbh9r	36	6	is	be	VBZ
cord-311035-s3tkbh9r	36	7	proteolytically	proteolytically	RB
cord-311035-s3tkbh9r	36	8	processed	process	VBN
cord-311035-s3tkbh9r	36	9	into	into	IN
cord-311035-s3tkbh9r	36	10	two	two	CD
cord-311035-s3tkbh9r	36	11	subunits	subunit	NNS
cord-311035-s3tkbh9r	36	12	,	,	,
cord-311035-s3tkbh9r	36	13	S1	S1	NNP
cord-311035-s3tkbh9r	36	14	and	and	CC
cord-311035-s3tkbh9r	36	15	S2	S2	NNP
cord-311035-s3tkbh9r	36	16	,	,	,
cord-311035-s3tkbh9r	36	17	that	that	WDT
cord-311035-s3tkbh9r	36	18	are	be	VBP
cord-311035-s3tkbh9r	36	19	non	non	JJ
cord-311035-s3tkbh9r	36	20	-	-	JJ
cord-311035-s3tkbh9r	36	21	covalently	covalently	RB
cord-311035-s3tkbh9r	36	22	associated	associate	VBN
cord-311035-s3tkbh9r	36	23	and	and	CC
cord-311035-s3tkbh9r	36	24	decorate	decorate	VB
cord-311035-s3tkbh9r	36	25	the	the	DT
cord-311035-s3tkbh9r	36	26	coronavirus	coronavirus	NN
cord-311035-s3tkbh9r	36	27	envelope	envelope	NN
cord-311035-s3tkbh9r	36	28	[	[	-LRB-
cord-311035-s3tkbh9r	36	29	31	31	CD
cord-311035-s3tkbh9r	36	30	]	]	-RRB-
cord-311035-s3tkbh9r	37	1	[	[	-LRB-
cord-311035-s3tkbh9r	37	2	32	32	CD
cord-311035-s3tkbh9r	37	3	]	]	-RRB-
cord-311035-s3tkbh9r	37	4	[	[	-LRB-
cord-311035-s3tkbh9r	37	5	33	33	CD
cord-311035-s3tkbh9r	37	6	]	]	-RRB-
cord-311035-s3tkbh9r	37	7	.	.	.
cord-311035-s3tkbh9r	38	1	S	S	NNP
cord-311035-s3tkbh9r	38	2	recognizes	recognize	VBZ
cord-311035-s3tkbh9r	38	3	angiotensin	angiotensin	NN
cord-311035-s3tkbh9r	38	4	-	-	HYPH
cord-311035-s3tkbh9r	38	5	converting	convert	VBG
cord-311035-s3tkbh9r	38	6	enzyme	enzyme	NN
cord-311035-s3tkbh9r	38	7	2	2	CD
cord-311035-s3tkbh9r	38	8	(	(	-LRB-
cord-311035-s3tkbh9r	38	9	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	38	10	)	)	-RRB-
cord-311035-s3tkbh9r	38	11	on	on	IN
cord-311035-s3tkbh9r	38	12	host	host	NN
cord-311035-s3tkbh9r	38	13	cells	cell	NNS
cord-311035-s3tkbh9r	38	14	to	to	TO
cord-311035-s3tkbh9r	38	15	initiate	initiate	VB
cord-311035-s3tkbh9r	38	16	attachment	attachment	NN
cord-311035-s3tkbh9r	38	17	and	and	CC
cord-311035-s3tkbh9r	38	18	fusion	fusion	NN
cord-311035-s3tkbh9r	38	19	of	of	IN
cord-311035-s3tkbh9r	38	20	the	the	DT
cord-311035-s3tkbh9r	38	21	viral	viral	JJ
cord-311035-s3tkbh9r	38	22	and	and	CC
cord-311035-s3tkbh9r	38	23	plasma	plasma	NN
cord-311035-s3tkbh9r	38	24	membranes	membrane	NNS
cord-311035-s3tkbh9r	38	25	[	[	-LRB-
cord-311035-s3tkbh9r	38	26	33	33	CD
cord-311035-s3tkbh9r	38	27	]	]	-RRB-
cord-311035-s3tkbh9r	38	28	[	[	-LRB-
cord-311035-s3tkbh9r	38	29	34	34	CD
cord-311035-s3tkbh9r	38	30	]	]	-RRB-
cord-311035-s3tkbh9r	38	31	[	[	-LRB-
cord-311035-s3tkbh9r	38	32	35	35	CD
cord-311035-s3tkbh9r	38	33	]	]	-RRB-
cord-311035-s3tkbh9r	38	34	[	[	-LRB-
cord-311035-s3tkbh9r	38	35	36	36	CD
cord-311035-s3tkbh9r	38	36	]	]	-RRB-
cord-311035-s3tkbh9r	38	37	[	[	-LRB-
cord-311035-s3tkbh9r	38	38	37	37	CD
cord-311035-s3tkbh9r	38	39	]	]	-RRB-
cord-311035-s3tkbh9r	38	40	[	[	-LRB-
cord-311035-s3tkbh9r	38	41	38	38	CD
cord-311035-s3tkbh9r	38	42	]	]	-RRB-
cord-311035-s3tkbh9r	38	43	.	.	.
cord-311035-s3tkbh9r	39	1	Soluble	soluble	JJ
cord-311035-s3tkbh9r	39	2	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	39	3	(	(	-LRB-
cord-311035-s3tkbh9r	39	4	sACE2	sACE2	NNP
cord-311035-s3tkbh9r	39	5	)	)	-RRB-
cord-311035-s3tkbh9r	39	6	blocks	block	VBZ
cord-311035-s3tkbh9r	39	7	receptor	receptor	NN
cord-311035-s3tkbh9r	39	8	-	-	HYPH
cord-311035-s3tkbh9r	39	9	binding	bind	VBG
cord-311035-s3tkbh9r	39	10	sites	site	NNS
cord-311035-s3tkbh9r	39	11	on	on	IN
cord-311035-s3tkbh9r	39	12	S	S	NNP
cord-311035-s3tkbh9r	39	13	[	[	-LRB-
cord-311035-s3tkbh9r	39	14	15	15	CD
cord-311035-s3tkbh9r	39	15	,	,	,
cord-311035-s3tkbh9r	39	16	37	37	CD
cord-311035-s3tkbh9r	39	17	,	,	,
cord-311035-s3tkbh9r	39	18	[	[	-LRB-
cord-311035-s3tkbh9r	39	19	39	39	CD
cord-311035-s3tkbh9r	39	20	]	]	-RRB-
cord-311035-s3tkbh9r	39	21	[	[	-LRB-
cord-311035-s3tkbh9r	39	22	40	40	CD
cord-311035-s3tkbh9r	39	23	]	]	-RRB-
cord-311035-s3tkbh9r	39	24	[	[	-LRB-
cord-311035-s3tkbh9r	39	25	41	41	CD
cord-311035-s3tkbh9r	39	26	]	]	-RRB-
cord-311035-s3tkbh9r	39	27	[	[	-LRB-
cord-311035-s3tkbh9r	39	28	42	42	CD
cord-311035-s3tkbh9r	39	29	]	]	-RRB-
cord-311035-s3tkbh9r	39	30	and	and	CC
cord-311035-s3tkbh9r	39	31	while	while	IN
cord-311035-s3tkbh9r	39	32	escape	escape	NN
cord-311035-s3tkbh9r	39	33	mutations	mutation	NNS
cord-311035-s3tkbh9r	39	34	in	in	IN
cord-311035-s3tkbh9r	39	35	S	S	NNP
cord-311035-s3tkbh9r	39	36	rapidly	rapidly	RB
cord-311035-s3tkbh9r	39	37	emerge	emerge	VBP
cord-311035-s3tkbh9r	39	38	in	in	IN
cord-311035-s3tkbh9r	39	39	tissue	tissue	NN
cord-311035-s3tkbh9r	39	40	culture	culture	NN
cord-311035-s3tkbh9r	39	41	in	in	IN
cord-311035-s3tkbh9r	39	42	the	the	DT
cord-311035-s3tkbh9r	39	43	presence	presence	NN
cord-311035-s3tkbh9r	39	44	of	of	IN
cord-311035-s3tkbh9r	39	45	monoclonal	monoclonal	JJ
cord-311035-s3tkbh9r	39	46	antibodies	antibody	NNS
cord-311035-s3tkbh9r	39	47	[	[	-LRB-
cord-311035-s3tkbh9r	39	48	43	43	CD
cord-311035-s3tkbh9r	39	49	]	]	-RRB-
cord-311035-s3tkbh9r	39	50	,	,	.
cord-311035-s3tkbh9r	40	1	in	in	IN
cord-311035-s3tkbh9r	40	2	principle	principle	NN
cord-311035-s3tkbh9r	40	3	the	the	DT
cord-311035-s3tkbh9r	40	4	virus	virus	NN
cord-311035-s3tkbh9r	40	5	has	have	VBZ
cord-311035-s3tkbh9r	40	6	limited	limit	VBN
cord-311035-s3tkbh9r	40	7	mechanisms	mechanism	NNS
cord-311035-s3tkbh9r	40	8	to	to	TO
cord-311035-s3tkbh9r	40	9	escape	escape	VB
cord-311035-s3tkbh9r	40	10	a	a	DT
cord-311035-s3tkbh9r	40	11	soluble	soluble	JJ
cord-311035-s3tkbh9r	40	12	decoy	decoy	NN
cord-311035-s3tkbh9r	40	13	receptor	receptor	NN
cord-311035-s3tkbh9r	40	14	without	without	IN
cord-311035-s3tkbh9r	40	15	simultaneously	simultaneously	RB
cord-311035-s3tkbh9r	40	16	losing	lose	VBG
cord-311035-s3tkbh9r	40	17	affinity	affinity	NN
cord-311035-s3tkbh9r	40	18	for	for	IN
cord-311035-s3tkbh9r	40	19	the	the	DT
cord-311035-s3tkbh9r	40	20	natural	natural	JJ
cord-311035-s3tkbh9r	40	21	receptor	receptor	NN
cord-311035-s3tkbh9r	40	22	.	.	.
cord-311035-s3tkbh9r	41	1	The	the	DT
cord-311035-s3tkbh9r	41	2	decoy	decoy	NN
cord-311035-s3tkbh9r	41	3	receptor	receptor	NN
cord-311035-s3tkbh9r	41	4	might	may	MD
cord-311035-s3tkbh9r	41	5	also	also	RB
cord-311035-s3tkbh9r	41	6	have	have	VB
cord-311035-s3tkbh9r	41	7	a	a	DT
cord-311035-s3tkbh9r	41	8	virucidal	virucidal	JJ
cord-311035-s3tkbh9r	41	9	effect	effect	NN
cord-311035-s3tkbh9r	41	10	by	by	IN
cord-311035-s3tkbh9r	41	11	inducing	induce	VBG
cord-311035-s3tkbh9r	41	12	conformational	conformational	JJ
cord-311035-s3tkbh9r	41	13	changes	change	NNS
cord-311035-s3tkbh9r	41	14	and	and	CC
cord-311035-s3tkbh9r	41	15	S1	s1	NN
cord-311035-s3tkbh9r	41	16	shedding	shedding	NN
cord-311035-s3tkbh9r	41	17	,	,	,
cord-311035-s3tkbh9r	41	18	such	such	JJ
cord-311035-s3tkbh9r	41	19	that	that	IN
cord-311035-s3tkbh9r	41	20	virus	virus	NN
cord-311035-s3tkbh9r	41	21	particles	particle	NNS
cord-311035-s3tkbh9r	41	22	are	be	VBP
cord-311035-s3tkbh9r	41	23	inactivated	inactivate	VBN
cord-311035-s3tkbh9r	41	24	even	even	RB
cord-311035-s3tkbh9r	41	25	if	if	IN
cord-311035-s3tkbh9r	41	26	sACE2	sACE2	NNP
cord-311035-s3tkbh9r	41	27	dissociates	dissociate	VBZ
cord-311035-s3tkbh9r	41	28	.	.	.
cord-311035-s3tkbh9r	42	1	However	however	RB
cord-311035-s3tkbh9r	42	2	,	,	,
cord-311035-s3tkbh9r	42	3	monoclonal	monoclonal	JJ
cord-311035-s3tkbh9r	42	4	antibodies	antibody	NNS
cord-311035-s3tkbh9r	42	5	have	have	VBP
cord-311035-s3tkbh9r	42	6	superior	superior	JJ
cord-311035-s3tkbh9r	42	7	affinity	affinity	NN
cord-311035-s3tkbh9r	42	8	and	and	CC
cord-311035-s3tkbh9r	42	9	neutralization	neutralization	NN
cord-311035-s3tkbh9r	42	10	efficacy	efficacy	NN
cord-311035-s3tkbh9r	42	11	.	.	.
cord-311035-s3tkbh9r	43	1	To	to	TO
cord-311035-s3tkbh9r	43	2	improve	improve	VB
cord-311035-s3tkbh9r	43	3	the	the	DT
cord-311035-s3tkbh9r	43	4	therapeutic	therapeutic	JJ
cord-311035-s3tkbh9r	43	5	potential	potential	NN
cord-311035-s3tkbh9r	43	6	of	of	IN
cord-311035-s3tkbh9r	43	7	decoy	decoy	NN
cord-311035-s3tkbh9r	43	8	receptors	receptor	NNS
cord-311035-s3tkbh9r	43	9	,	,	,
cord-311035-s3tkbh9r	43	10	my	-PRON-	PRP$
cord-311035-s3tkbh9r	43	11	group	group	NN
cord-311035-s3tkbh9r	43	12	used	use	VBD
cord-311035-s3tkbh9r	43	13	deep	deep	JJ
cord-311035-s3tkbh9r	43	14	mutagenesis	mutagenesis	NN
cord-311035-s3tkbh9r	43	15	to	to	TO
cord-311035-s3tkbh9r	43	16	find	find	VB
cord-311035-s3tkbh9r	43	17	mutations	mutation	NNS
cord-311035-s3tkbh9r	43	18	in	in	IN
cord-311035-s3tkbh9r	43	19	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	43	20	that	that	WDT
cord-311035-s3tkbh9r	43	21	enhance	enhance	VBP
cord-311035-s3tkbh9r	43	22	affinity	affinity	NN
cord-311035-s3tkbh9r	43	23	[	[	-LRB-
cord-311035-s3tkbh9r	43	24	15	15	CD
cord-311035-s3tkbh9r	43	25	]	]	-RRB-
cord-311035-s3tkbh9r	43	26	.	.	.
cord-311035-s3tkbh9r	44	1	A	a	DT
cord-311035-s3tkbh9r	44	2	library	library	NN
cord-311035-s3tkbh9r	44	3	of	of	IN
cord-311035-s3tkbh9r	44	4	over	over	IN
cord-311035-s3tkbh9r	44	5	2,000	2,000	CD
cord-311035-s3tkbh9r	44	6	single	single	JJ
cord-311035-s3tkbh9r	44	7	amino	amino	NN
cord-311035-s3tkbh9r	44	8	acid	acid	NN
cord-311035-s3tkbh9r	44	9	substitutions	substitution	NNS
cord-311035-s3tkbh9r	44	10	in	in	IN
cord-311035-s3tkbh9r	44	11	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	44	12	was	be	VBD
cord-311035-s3tkbh9r	44	13	constructed	construct	VBN
cord-311035-s3tkbh9r	44	14	,	,	,
cord-311035-s3tkbh9r	44	15	focused	focus	VBN
cord-311035-s3tkbh9r	44	16	on	on	IN
cord-311035-s3tkbh9r	44	17	diversification	diversification	NN
cord-311035-s3tkbh9r	44	18	of	of	IN
cord-311035-s3tkbh9r	44	19	residues	residue	NNS
cord-311035-s3tkbh9r	44	20	at	at	IN
cord-311035-s3tkbh9r	44	21	the	the	DT
cord-311035-s3tkbh9r	44	22	structurally	structurally	RB
cord-311035-s3tkbh9r	44	23	defined	define	VBN
cord-311035-s3tkbh9r	44	24	interface	interface	NN
cord-311035-s3tkbh9r	44	25	with	with	IN
cord-311035-s3tkbh9r	44	26	the	the	DT
cord-311035-s3tkbh9r	44	27	receptor	receptor	NN
cord-311035-s3tkbh9r	44	28	-	-	HYPH
cord-311035-s3tkbh9r	44	29	binding	bind	VBG
cord-311035-s3tkbh9r	44	30	domain	domain	NN
cord-311035-s3tkbh9r	44	31	(	(	-LRB-
cord-311035-s3tkbh9r	44	32	RBD	RBD	NNP
cord-311035-s3tkbh9r	44	33	)	)	-RRB-
cord-311035-s3tkbh9r	44	34	of	of	IN
cord-311035-s3tkbh9r	44	35	S	S	NNP
cord-311035-s3tkbh9r	44	36	[	[	-LRB-
cord-311035-s3tkbh9r	44	37	44	44	CD
cord-311035-s3tkbh9r	44	38	,	,	,
cord-311035-s3tkbh9r	44	39	45	45	CD
cord-311035-s3tkbh9r	44	40	]	]	-RRB-
cord-311035-s3tkbh9r	44	41	and	and	CC
cord-311035-s3tkbh9r	44	42	also	also	RB
cord-311035-s3tkbh9r	44	43	within	within	IN
cord-311035-s3tkbh9r	44	44	the	the	DT
cord-311035-s3tkbh9r	44	45	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	44	46	catalytic	catalytic	JJ
cord-311035-s3tkbh9r	44	47	cleft	cleft	NN
cord-311035-s3tkbh9r	44	48	.	.	.
cord-311035-s3tkbh9r	45	1	The	the	DT
cord-311035-s3tkbh9r	45	2	library	library	NN
cord-311035-s3tkbh9r	45	3	was	be	VBD
cord-311035-s3tkbh9r	45	4	expressed	express	VBN
cord-311035-s3tkbh9r	45	5	in	in	IN
cord-311035-s3tkbh9r	45	6	a	a	DT
cord-311035-s3tkbh9r	45	7	human	human	JJ
cord-311035-s3tkbh9r	45	8	cell	cell	NN
cord-311035-s3tkbh9r	45	9	line	line	NN
cord-311035-s3tkbh9r	45	10	,	,	,
cord-311035-s3tkbh9r	45	11	with	with	IN
cord-311035-s3tkbh9r	45	12	a	a	DT
cord-311035-s3tkbh9r	45	13	c	c	NNP
cord-311035-s3tkbh9r	45	14	-	-	HYPH
cord-311035-s3tkbh9r	45	15	myc	myc	NNP
cord-311035-s3tkbh9r	45	16	epitope	epitope	NN
cord-311035-s3tkbh9r	45	17	tag	tag	NN
cord-311035-s3tkbh9r	45	18	fused	fuse	VBN
cord-311035-s3tkbh9r	45	19	to	to	IN
cord-311035-s3tkbh9r	45	20	the	the	DT
cord-311035-s3tkbh9r	45	21	extracellular	extracellular	JJ
cord-311035-s3tkbh9r	45	22	N	n	CD
cord-311035-s3tkbh9r	45	23	-	-	HYPH
cord-311035-s3tkbh9r	45	24	terminus	terminus	NN
cord-311035-s3tkbh9r	45	25	of	of	IN
cord-311035-s3tkbh9r	45	26	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	45	27	for	for	IN
cord-311035-s3tkbh9r	45	28	detection	detection	NN
cord-311035-s3tkbh9r	45	29	of	of	IN
cord-311035-s3tkbh9r	45	30	surface	surface	NN
cord-311035-s3tkbh9r	45	31	expressed	express	VBD
cord-311035-s3tkbh9r	45	32	protein	protein	NN
cord-311035-s3tkbh9r	45	33	.	.	.
cord-311035-s3tkbh9r	46	1	Other	other	JJ
cord-311035-s3tkbh9r	46	2	than	than	IN
cord-311035-s3tkbh9r	46	3	the	the	DT
cord-311035-s3tkbh9r	46	4	presence	presence	NN
cord-311035-s3tkbh9r	46	5	of	of	IN
cord-311035-s3tkbh9r	46	6	the	the	DT
cord-311035-s3tkbh9r	46	7	epitope	epitope	NN
cord-311035-s3tkbh9r	46	8	tag	tag	NN
cord-311035-s3tkbh9r	46	9	,	,	,
cord-311035-s3tkbh9r	46	10	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	46	11	expressed	express	VBD
cord-311035-s3tkbh9r	46	12	in	in	IN
cord-311035-s3tkbh9r	46	13	this	this	DT
cord-311035-s3tkbh9r	46	14	experimental	experimental	JJ
cord-311035-s3tkbh9r	46	15	selection	selection	NN
cord-311035-s3tkbh9r	46	16	system	system	NN
cord-311035-s3tkbh9r	46	17	matches	match	VBZ
cord-311035-s3tkbh9r	46	18	native	native	JJ
cord-311035-s3tkbh9r	46	19	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	46	20	in	in	IN
cord-311035-s3tkbh9r	46	21	the	the	DT
cord-311035-s3tkbh9r	46	22	human	human	JJ
cord-311035-s3tkbh9r	46	23	body	body	NN
cord-311035-s3tkbh9r	46	24	.	.	.
cord-311035-s3tkbh9r	47	1	The	the	DT
cord-311035-s3tkbh9r	47	2	culture	culture	NN
cord-311035-s3tkbh9r	47	3	expressing	express	VBG
cord-311035-s3tkbh9r	47	4	the	the	DT
cord-311035-s3tkbh9r	47	5	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	47	6	library	library	NN
cord-311035-s3tkbh9r	47	7	was	be	VBD
cord-311035-s3tkbh9r	47	8	then	then	RB
cord-311035-s3tkbh9r	47	9	selected	select	VBN
cord-311035-s3tkbh9r	47	10	by	by	IN
cord-311035-s3tkbh9r	47	11	fluorescence	fluorescence	NN
cord-311035-s3tkbh9r	47	12	activated	activate	VBN
cord-311035-s3tkbh9r	47	13	cell	cell	NN
cord-311035-s3tkbh9r	47	14	sorting	sorting	NN
cord-311035-s3tkbh9r	47	15	(	(	-LRB-
cord-311035-s3tkbh9r	47	16	FACS	FACS	NNP
cord-311035-s3tkbh9r	47	17	)	)	-RRB-
cord-311035-s3tkbh9r	47	18	to	to	TO
cord-311035-s3tkbh9r	47	19	collect	collect	VB
cord-311035-s3tkbh9r	47	20	cells	cell	NNS
cord-311035-s3tkbh9r	47	21	expressing	express	VBG
cord-311035-s3tkbh9r	47	22	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	47	23	variants	variant	NNS
cord-311035-s3tkbh9r	47	24	with	with	IN
cord-311035-s3tkbh9r	47	25	tight	tight	JJ
cord-311035-s3tkbh9r	47	26	affinity	affinity	NN
cord-311035-s3tkbh9r	47	27	for	for	IN
cord-311035-s3tkbh9r	47	28	fluorescently	fluorescently	RB
cord-311035-s3tkbh9r	47	29	labeled	label	VBN
cord-311035-s3tkbh9r	47	30	RBD	RBD	NNP
cord-311035-s3tkbh9r	47	31	from	from	IN
cord-311035-s3tkbh9r	47	32	S	S	NNP
cord-311035-s3tkbh9r	47	33	of	of	IN
cord-311035-s3tkbh9r	47	34	SARS	SARS	NNP
cord-311035-s3tkbh9r	47	35	-	-	HYPH
cord-311035-s3tkbh9r	47	36	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	48	1	(	(	-LRB-
cord-311035-s3tkbh9r	48	2	Figure	figure	NN
cord-311035-s3tkbh9r	48	3	1A	1a	NN
cord-311035-s3tkbh9r	48	4	)	)	-RRB-
cord-311035-s3tkbh9r	48	5	.	.	.
cord-311035-s3tkbh9r	49	1	For	for	IN
cord-311035-s3tkbh9r	49	2	the	the	DT
cord-311035-s3tkbh9r	49	3	artificial	artificial	JJ
cord-311035-s3tkbh9r	49	4	selection	selection	NN
cord-311035-s3tkbh9r	49	5	to	to	TO
cord-311035-s3tkbh9r	49	6	be	be	VB
cord-311035-s3tkbh9r	49	7	successful	successful	JJ
cord-311035-s3tkbh9r	49	8	,	,	,
cord-311035-s3tkbh9r	49	9	cells	cell	NNS
cord-311035-s3tkbh9r	49	10	must	must	MD
cord-311035-s3tkbh9r	49	11	express	express	VB
cord-311035-s3tkbh9r	49	12	a	a	DT
cord-311035-s3tkbh9r	49	13	single	single	JJ
cord-311035-s3tkbh9r	49	14	protein	protein	NN
cord-311035-s3tkbh9r	49	15	variant	variant	NN
cord-311035-s3tkbh9r	49	16	from	from	IN
cord-311035-s3tkbh9r	49	17	a	a	DT
cord-311035-s3tkbh9r	49	18	single	single	JJ
cord-311035-s3tkbh9r	49	19	sequence	sequence	NN
cord-311035-s3tkbh9r	49	20	variant	variant	NN
cord-311035-s3tkbh9r	49	21	,	,	,
cord-311035-s3tkbh9r	49	22	thereby	thereby	RB
cord-311035-s3tkbh9r	49	23	providing	provide	VBG
cord-311035-s3tkbh9r	49	24	a	a	DT
cord-311035-s3tkbh9r	49	25	tight	tight	JJ
cord-311035-s3tkbh9r	49	26	physical	physical	JJ
cord-311035-s3tkbh9r	49	27	link	link	NN
cord-311035-s3tkbh9r	49	28	between	between	IN
cord-311035-s3tkbh9r	49	29	the	the	DT
cord-311035-s3tkbh9r	49	30	phenotype	phenotype	NN
cord-311035-s3tkbh9r	49	31	of	of	IN
cord-311035-s3tkbh9r	49	32	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	49	33	expressed	express	VBD
cord-311035-s3tkbh9r	49	34	at	at	IN
cord-311035-s3tkbh9r	49	35	the	the	DT
cord-311035-s3tkbh9r	49	36	plasma	plasma	NN
cord-311035-s3tkbh9r	49	37	membrane	membrane	NN
cord-311035-s3tkbh9r	49	38	and	and	CC
cord-311035-s3tkbh9r	49	39	a	a	DT
cord-311035-s3tkbh9r	49	40	single	single	JJ
cord-311035-s3tkbh9r	49	41	sequence	sequence	NN
cord-311035-s3tkbh9r	49	42	within	within	IN
cord-311035-s3tkbh9r	49	43	the	the	DT
cord-311035-s3tkbh9r	49	44	cell	cell	NN
cord-311035-s3tkbh9r	49	45	.	.	.
cord-311035-s3tkbh9r	50	1	Getting	get	VBG
cord-311035-s3tkbh9r	50	2	human	human	JJ
cord-311035-s3tkbh9r	50	3	cells	cell	NNS
cord-311035-s3tkbh9r	50	4	in	in	IN
cord-311035-s3tkbh9r	50	5	culture	culture	NN
cord-311035-s3tkbh9r	50	6	to	to	TO
cord-311035-s3tkbh9r	50	7	acquire	acquire	VB
cord-311035-s3tkbh9r	50	8	and	and	CC
cord-311035-s3tkbh9r	50	9	express	express	VB
cord-311035-s3tkbh9r	50	10	a	a	DT
cord-311035-s3tkbh9r	50	11	single	single	JJ
cord-311035-s3tkbh9r	50	12	coding	code	VBG
cord-311035-s3tkbh9r	50	13	variant	variant	NN
cord-311035-s3tkbh9r	50	14	is	be	VBZ
cord-311035-s3tkbh9r	50	15	no	no	DT
cord-311035-s3tkbh9r	50	16	trivial	trivial	JJ
cord-311035-s3tkbh9r	50	17	feat	feat	NN
cord-311035-s3tkbh9r	50	18	,	,	,
cord-311035-s3tkbh9r	50	19	as	as	IN
cord-311035-s3tkbh9r	50	20	transfection	transfection	NN
cord-311035-s3tkbh9r	50	21	methods	method	NNS
cord-311035-s3tkbh9r	50	22	typically	typically	RB
cord-311035-s3tkbh9r	50	23	introduce	introduce	VBP
cord-311035-s3tkbh9r	50	24	many	many	JJ
cord-311035-s3tkbh9r	50	25	plasmid	plasmid	NN
cord-311035-s3tkbh9r	50	26	copies	copy	NNS
cord-311035-s3tkbh9r	50	27	.	.	.
cord-311035-s3tkbh9r	51	1	Different	different	JJ
cord-311035-s3tkbh9r	51	2	methods	method	NNS
cord-311035-s3tkbh9r	51	3	to	to	TO
cord-311035-s3tkbh9r	51	4	solve	solve	VB
cord-311035-s3tkbh9r	51	5	this	this	DT
cord-311035-s3tkbh9r	51	6	technical	technical	JJ
cord-311035-s3tkbh9r	51	7	challenge	challenge	NN
cord-311035-s3tkbh9r	51	8	have	have	VBP
cord-311035-s3tkbh9r	51	9	included	include	VBN
cord-311035-s3tkbh9r	51	10	the	the	DT
cord-311035-s3tkbh9r	51	11	use	use	NN
cord-311035-s3tkbh9r	51	12	of	of	IN
cord-311035-s3tkbh9r	51	13	episomal	episomal	JJ
cord-311035-s3tkbh9r	51	14	plasmids	plasmid	NNS
cord-311035-s3tkbh9r	51	15	that	that	WDT
cord-311035-s3tkbh9r	51	16	randomly	randomly	RB
cord-311035-s3tkbh9r	51	17	partition	partition	VBP
cord-311035-s3tkbh9r	51	18	to	to	IN
cord-311035-s3tkbh9r	51	19	daughter	daughter	NN
cord-311035-s3tkbh9r	51	20	cells	cell	NNS
cord-311035-s3tkbh9r	51	21	during	during	IN
cord-311035-s3tkbh9r	51	22	division	division	NN
cord-311035-s3tkbh9r	51	23	until	until	IN
cord-311035-s3tkbh9r	51	24	progeny	progeny	NN
cord-311035-s3tkbh9r	51	25	harbor	harbor	VBP
cord-311035-s3tkbh9r	51	26	a	a	DT
cord-311035-s3tkbh9r	51	27	single	single	JJ
cord-311035-s3tkbh9r	51	28	coding	code	VBG
cord-311035-s3tkbh9r	51	29	variant	variant	NN
cord-311035-s3tkbh9r	51	30	over	over	IN
cord-311035-s3tkbh9r	51	31	time	time	NN
cord-311035-s3tkbh9r	51	32	[	[	-LRB-
cord-311035-s3tkbh9r	51	33	4	4	CD
cord-311035-s3tkbh9r	51	34	]	]	-RRB-
cord-311035-s3tkbh9r	51	35	,	,	,
cord-311035-s3tkbh9r	51	36	the	the	DT
cord-311035-s3tkbh9r	51	37	use	use	NN
cord-311035-s3tkbh9r	51	38	of	of	IN
cord-311035-s3tkbh9r	51	39	engineered	engineer	VBN
cord-311035-s3tkbh9r	51	40	integration	integration	NN
cord-311035-s3tkbh9r	51	41	sites	site	NNS
cord-311035-s3tkbh9r	51	42	in	in	IN
cord-311035-s3tkbh9r	51	43	the	the	DT
cord-311035-s3tkbh9r	51	44	genome	genome	NN
cord-311035-s3tkbh9r	51	45	[	[	-LRB-
cord-311035-s3tkbh9r	51	46	9	9	CD
cord-311035-s3tkbh9r	51	47	,	,	,
cord-311035-s3tkbh9r	51	48	46	46	CD
cord-311035-s3tkbh9r	51	49	,	,	,
cord-311035-s3tkbh9r	51	50	47	47	CD
cord-311035-s3tkbh9r	51	51	]	]	-RRB-
cord-311035-s3tkbh9r	51	52	,	,	,
cord-311035-s3tkbh9r	51	53	or	or	CC
cord-311035-s3tkbh9r	51	54	the	the	DT
cord-311035-s3tkbh9r	51	55	use	use	NN
cord-311035-s3tkbh9r	51	56	of	of	IN
cord-311035-s3tkbh9r	51	57	viral	viral	JJ
cord-311035-s3tkbh9r	51	58	vectors	vector	NNS
cord-311035-s3tkbh9r	51	59	at	at	IN
cord-311035-s3tkbh9r	51	60	low	low	JJ
cord-311035-s3tkbh9r	51	61	multiplicities	multiplicity	NNS
cord-311035-s3tkbh9r	51	62	-	-	HYPH
cord-311035-s3tkbh9r	51	63	of	of	IN
cord-311035-s3tkbh9r	51	64	-	-	HYPH
cord-311035-s3tkbh9r	51	65	infection	infection	NN
cord-311035-s3tkbh9r	51	66	[	[	-LRB-
cord-311035-s3tkbh9r	51	67	48	48	CD
cord-311035-s3tkbh9r	51	68	,	,	,
cord-311035-s3tkbh9r	51	69	49	49	CD
cord-311035-s3tkbh9r	51	70	]	]	-RRB-
cord-311035-s3tkbh9r	51	71	.	.	.
cord-311035-s3tkbh9r	52	1	My	-PRON-	PRP$
cord-311035-s3tkbh9r	52	2	group	group	NN
cord-311035-s3tkbh9r	52	3	used	use	VBD
cord-311035-s3tkbh9r	52	4	carrier	carrier	NN
cord-311035-s3tkbh9r	52	5	DNA	DNA	NNP
cord-311035-s3tkbh9r	52	6	to	to	TO
cord-311035-s3tkbh9r	52	7	sufficiently	sufficiently	RB
cord-311035-s3tkbh9r	52	8	dilute	dilute	VB
cord-311035-s3tkbh9r	52	9	the	the	DT
cord-311035-s3tkbh9r	52	10	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	52	11	plasmid	plasmid	NN
cord-311035-s3tkbh9r	52	12	library	library	NN
cord-311035-s3tkbh9r	52	13	such	such	JJ
cord-311035-s3tkbh9r	52	14	that	that	IN
cord-311035-s3tkbh9r	52	15	each	each	DT
cord-311035-s3tkbh9r	52	16	cell	cell	NN
cord-311035-s3tkbh9r	52	17	typically	typically	RB
cord-311035-s3tkbh9r	52	18	acquired	acquire	VBD
cord-311035-s3tkbh9r	52	19	no	no	DT
cord-311035-s3tkbh9r	52	20	more	more	JJR
cord-311035-s3tkbh9r	52	21	than	than	IN
cord-311035-s3tkbh9r	52	22	a	a	DT
cord-311035-s3tkbh9r	52	23	single	single	JJ
cord-311035-s3tkbh9r	52	24	coding	code	VBG
cord-311035-s3tkbh9r	52	25	variant	variant	NN
cord-311035-s3tkbh9r	52	26	[	[	-LRB-
cord-311035-s3tkbh9r	52	27	11	11	CD
cord-311035-s3tkbh9r	52	28	]	]	-RRB-
cord-311035-s3tkbh9r	52	29	.	.	.
cord-311035-s3tkbh9r	53	1	An	an	DT
cord-311035-s3tkbh9r	53	2	episomal	episomal	JJ
cord-311035-s3tkbh9r	53	3	plasmid	plasmid	NN
cord-311035-s3tkbh9r	53	4	is	be	VBZ
cord-311035-s3tkbh9r	53	5	used	use	VBN
cord-311035-s3tkbh9r	53	6	for	for	IN
cord-311035-s3tkbh9r	53	7	the	the	DT
cord-311035-s3tkbh9r	53	8	library	library	NN
cord-311035-s3tkbh9r	53	9	so	so	IN
cord-311035-s3tkbh9r	53	10	that	that	IN
cord-311035-s3tkbh9r	53	11	extrachromosomal	extrachromosomal	VBN
cord-311035-s3tkbh9r	53	12	replication	replication	NN
cord-311035-s3tkbh9r	53	13	within	within	IN
cord-311035-s3tkbh9r	53	14	the	the	DT
cord-311035-s3tkbh9r	53	15	cell	cell	NN
cord-311035-s3tkbh9r	53	16	enhances	enhance	VBZ
cord-311035-s3tkbh9r	53	17	expression	expression	NN
cord-311035-s3tkbh9r	53	18	of	of	IN
cord-311035-s3tkbh9r	53	19	the	the	DT
cord-311035-s3tkbh9r	53	20	protein	protein	NN
cord-311035-s3tkbh9r	53	21	under	under	IN
cord-311035-s3tkbh9r	53	22	investigation	investigation	NN
cord-311035-s3tkbh9r	53	23	.	.	.
cord-311035-s3tkbh9r	54	1	(	(	-LRB-
cord-311035-s3tkbh9r	54	2	The	the	DT
cord-311035-s3tkbh9r	54	3	carrier	carrier	NN
cord-311035-s3tkbh9r	54	4	DNA	dna	NN
cord-311035-s3tkbh9r	54	5	,	,	,
cord-311035-s3tkbh9r	54	6	itself	-PRON-	PRP
cord-311035-s3tkbh9r	54	7	a	a	DT
cord-311035-s3tkbh9r	54	8	modified	modify	VBN
cord-311035-s3tkbh9r	54	9	episomal	episomal	JJ
cord-311035-s3tkbh9r	54	10	plasmid	plasmid	NN
cord-311035-s3tkbh9r	54	11	,	,	,
cord-311035-s3tkbh9r	54	12	further	further	RB
cord-311035-s3tkbh9r	54	13	assists	assist	NNS
cord-311035-s3tkbh9r	54	14	in	in	IN
cord-311035-s3tkbh9r	54	15	this	this	DT
cord-311035-s3tkbh9r	54	16	process	process	NN
cord-311035-s3tkbh9r	54	17	[	[	-LRB-
cord-311035-s3tkbh9r	54	18	50	50	CD
cord-311035-s3tkbh9r	54	19	]	]	-RRB-
cord-311035-s3tkbh9r	54	20	.	.	.
cord-311035-s3tkbh9r	54	21	)	)	-RRB-
cord-311035-s3tkbh9r	55	1	The	the	DT
cord-311035-s3tkbh9r	55	2	disadvantage	disadvantage	NN
cord-311035-s3tkbh9r	55	3	to	to	IN
cord-311035-s3tkbh9r	55	4	this	this	DT
cord-311035-s3tkbh9r	55	5	simple	simple	JJ
cord-311035-s3tkbh9r	55	6	solution	solution	NN
cord-311035-s3tkbh9r	55	7	for	for	IN
cord-311035-s3tkbh9r	55	8	linking	link	VBG
cord-311035-s3tkbh9r	55	9	a	a	DT
cord-311035-s3tkbh9r	55	10	single	single	JJ
cord-311035-s3tkbh9r	55	11	genotype	genotype	NN
cord-311035-s3tkbh9r	55	12	to	to	IN
cord-311035-s3tkbh9r	55	13	phenotype	phenotype	NN
cord-311035-s3tkbh9r	55	14	is	be	VBZ
cord-311035-s3tkbh9r	55	15	that	that	IN
cord-311035-s3tkbh9r	55	16	the	the	DT
cord-311035-s3tkbh9r	55	17	coding	code	VBG
cord-311035-s3tkbh9r	55	18	sequence	sequence	NN
cord-311035-s3tkbh9r	55	19	is	be	VBZ
cord-311035-s3tkbh9r	55	20	so	so	RB
cord-311035-s3tkbh9r	55	21	diluted	dilute	VBN
cord-311035-s3tkbh9r	55	22	with	with	IN
cord-311035-s3tkbh9r	55	23	carrier	carrier	NN
cord-311035-s3tkbh9r	55	24	DNA	dna	NN
cord-311035-s3tkbh9r	55	25	,	,	,
cord-311035-s3tkbh9r	55	26	most	most	JJS
cord-311035-s3tkbh9r	55	27	cells	cell	NNS
cord-311035-s3tkbh9r	55	28	in	in	IN
cord-311035-s3tkbh9r	55	29	the	the	DT
cord-311035-s3tkbh9r	55	30	(	(	-LRB-
cord-311035-s3tkbh9r	55	31	A	a	NN
cord-311035-s3tkbh9r	55	32	)	)	-RRB-
cord-311035-s3tkbh9r	55	33	A	a	DT
cord-311035-s3tkbh9r	55	34	library	library	NN
cord-311035-s3tkbh9r	55	35	of	of	IN
cord-311035-s3tkbh9r	55	36	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	55	37	variants	variant	NNS
cord-311035-s3tkbh9r	55	38	was	be	VBD
cord-311035-s3tkbh9r	55	39	expressed	express	VBN
cord-311035-s3tkbh9r	55	40	in	in	IN
cord-311035-s3tkbh9r	55	41	human	human	JJ
cord-311035-s3tkbh9r	55	42	cells	cell	NNS
cord-311035-s3tkbh9r	55	43	.	.	.
cord-311035-s3tkbh9r	56	1	Full	full	JJ
cord-311035-s3tkbh9r	56	2	-	-	HYPH
cord-311035-s3tkbh9r	56	3	length	length	NN
cord-311035-s3tkbh9r	56	4	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	56	5	(	(	-LRB-
cord-311035-s3tkbh9r	56	6	tan	tan	NNP
cord-311035-s3tkbh9r	56	7	)	)	-RRB-
cord-311035-s3tkbh9r	56	8	was	be	VBD
cord-311035-s3tkbh9r	56	9	tagged	tag	VBN
cord-311035-s3tkbh9r	56	10	with	with	IN
cord-311035-s3tkbh9r	56	11	a	a	DT
cord-311035-s3tkbh9r	56	12	c	c	NNP
cord-311035-s3tkbh9r	56	13	-	-	HYPH
cord-311035-s3tkbh9r	56	14	myc	myc	NNP
cord-311035-s3tkbh9r	56	15	epitope	epitope	NN
cord-311035-s3tkbh9r	56	16	at	at	IN
cord-311035-s3tkbh9r	56	17	its	-PRON-	PRP$
cord-311035-s3tkbh9r	56	18	extracellular	extracellular	JJ
cord-311035-s3tkbh9r	56	19	N	n	CD
cord-311035-s3tkbh9r	56	20	-	-	HYPH
cord-311035-s3tkbh9r	56	21	terminus	terminus	NN
cord-311035-s3tkbh9r	56	22	for	for	IN
cord-311035-s3tkbh9r	56	23	detection	detection	NN
cord-311035-s3tkbh9r	56	24	of	of	IN
cord-311035-s3tkbh9r	56	25	surface	surface	NN
cord-311035-s3tkbh9r	56	26	expression	expression	NN
cord-311035-s3tkbh9r	56	27	with	with	IN
cord-311035-s3tkbh9r	56	28	a	a	DT
cord-311035-s3tkbh9r	56	29	fluorescent	fluorescent	JJ
cord-311035-s3tkbh9r	56	30	antibody	antibody	NN
cord-311035-s3tkbh9r	56	31	(	(	-LRB-
cord-311035-s3tkbh9r	56	32	red	red	NNP
cord-311035-s3tkbh9r	56	33	)	)	-RRB-
cord-311035-s3tkbh9r	56	34	.	.	.
cord-311035-s3tkbh9r	57	1	SARS	SARS	NNP
cord-311035-s3tkbh9r	57	2	-	-	HYPH
cord-311035-s3tkbh9r	57	3	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	57	4	RBD	RBD	NNP
cord-311035-s3tkbh9r	58	1	(	(	-LRB-
cord-311035-s3tkbh9r	58	2	pale	pale	JJ
cord-311035-s3tkbh9r	58	3	green	green	JJ
cord-311035-s3tkbh9r	58	4	)	)	-RRB-
cord-311035-s3tkbh9r	58	5	genetically	genetically	RB
cord-311035-s3tkbh9r	58	6	fused	fuse	VBN
cord-311035-s3tkbh9r	58	7	with	with	IN
cord-311035-s3tkbh9r	58	8	superfolder	superfolder	NN
cord-311035-s3tkbh9r	58	9	green	green	JJ
cord-311035-s3tkbh9r	58	10	fluorescent	fluorescent	NN
cord-311035-s3tkbh9r	58	11	protein	protein	NN
cord-311035-s3tkbh9r	58	12	(	(	-LRB-
cord-311035-s3tkbh9r	58	13	sfGFP	sfGFP	NNP
cord-311035-s3tkbh9r	58	14	;	;	:
cord-311035-s3tkbh9r	58	15	dark	dark	JJ
cord-311035-s3tkbh9r	58	16	green	green	NNP
cord-311035-s3tkbh9r	58	17	)	)	-RRB-
cord-311035-s3tkbh9r	58	18	was	be	VBD
cord-311035-s3tkbh9r	58	19	incubated	incubate	VBN
cord-311035-s3tkbh9r	58	20	with	with	IN
cord-311035-s3tkbh9r	58	21	the	the	DT
cord-311035-s3tkbh9r	58	22	cell	cell	NN
cord-311035-s3tkbh9r	58	23	culture	culture	NN
cord-311035-s3tkbh9r	58	24	.	.	.
cord-311035-s3tkbh9r	59	1	FACS	FACS	NNP
cord-311035-s3tkbh9r	59	2	was	be	VBD
cord-311035-s3tkbh9r	59	3	used	use	VBN
cord-311035-s3tkbh9r	59	4	to	to	TO
cord-311035-s3tkbh9r	59	5	collect	collect	VB
cord-311035-s3tkbh9r	59	6	fluorescent	fluorescent	NN
cord-311035-s3tkbh9r	59	7	cells	cell	NNS
cord-311035-s3tkbh9r	59	8	expressing	express	VBG
cord-311035-s3tkbh9r	59	9	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	59	10	with	with	IN
cord-311035-s3tkbh9r	59	11	bound	bind	VBN
cord-311035-s3tkbh9r	59	12	RBD	RBD	NNP
cord-311035-s3tkbh9r	59	13	-	-	HYPH
cord-311035-s3tkbh9r	59	14	sfGFP	sfGFP	NNP
cord-311035-s3tkbh9r	59	15	.	.	.
cord-311035-s3tkbh9r	60	1	(	(	-LRB-
cord-311035-s3tkbh9r	60	2	B	b	NN
cord-311035-s3tkbh9r	60	3	)	)	-RRB-
cord-311035-s3tkbh9r	61	1	The	the	DT
cord-311035-s3tkbh9r	61	2	isolated	isolated	JJ
cord-311035-s3tkbh9r	61	3	RBD	RBD	NNP
cord-311035-s3tkbh9r	61	4	of	of	IN
cord-311035-s3tkbh9r	61	5	SARS	SARS	NNP
cord-311035-s3tkbh9r	61	6	-	-	HYPH
cord-311035-s3tkbh9r	61	7	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	61	8	(	(	-LRB-
cord-311035-s3tkbh9r	61	9	pale	pale	JJ
cord-311035-s3tkbh9r	61	10	green	green	JJ
cord-311035-s3tkbh9r	61	11	)	)	-RRB-
cord-311035-s3tkbh9r	61	12	was	be	VBD
cord-311035-s3tkbh9r	61	13	fused	fuse	VBN
cord-311035-s3tkbh9r	61	14	at	at	IN
cord-311035-s3tkbh9r	61	15	its	-PRON-	PRP$
cord-311035-s3tkbh9r	61	16	N	n	CD
cord-311035-s3tkbh9r	61	17	-	-	HYPH
cord-311035-s3tkbh9r	61	18	terminus	terminus	NN
cord-311035-s3tkbh9r	61	19	to	to	IN
cord-311035-s3tkbh9r	61	20	Aga2p	Aga2p	NNP
cord-311035-s3tkbh9r	61	21	(	(	-LRB-
cord-311035-s3tkbh9r	61	22	blue	blue	NNP
cord-311035-s3tkbh9r	61	23	)	)	-RRB-
cord-311035-s3tkbh9r	61	24	and	and	CC
cord-311035-s3tkbh9r	61	25	at	at	IN
cord-311035-s3tkbh9r	61	26	its	-PRON-	PRP$
cord-311035-s3tkbh9r	61	27	C	c	NN
cord-311035-s3tkbh9r	61	28	-	-	HYPH
cord-311035-s3tkbh9r	61	29	terminus	terminus	NN
cord-311035-s3tkbh9r	61	30	to	to	IN
cord-311035-s3tkbh9r	61	31	a	a	DT
cord-311035-s3tkbh9r	61	32	c	c	NNP
cord-311035-s3tkbh9r	61	33	-	-	HYPH
cord-311035-s3tkbh9r	61	34	myc	myc	NNP
cord-311035-s3tkbh9r	61	35	epitope	epitope	NN
cord-311035-s3tkbh9r	61	36	tag	tag	NN
cord-311035-s3tkbh9r	61	37	.	.	.
cord-311035-s3tkbh9r	62	1	A	a	DT
cord-311035-s3tkbh9r	62	2	saturation	saturation	NN
cord-311035-s3tkbh9r	62	3	mutagenesis	mutagenesis	NN
cord-311035-s3tkbh9r	62	4	library	library	NN
cord-311035-s3tkbh9r	62	5	of	of	IN
cord-311035-s3tkbh9r	62	6	the	the	DT
cord-311035-s3tkbh9r	62	7	RBD	RBD	NNP
cord-311035-s3tkbh9r	62	8	was	be	VBD
cord-311035-s3tkbh9r	62	9	expressed	express	VBN
cord-311035-s3tkbh9r	62	10	on	on	IN
cord-311035-s3tkbh9r	62	11	the	the	DT
cord-311035-s3tkbh9r	62	12	yeast	yeast	NN
cord-311035-s3tkbh9r	62	13	surface	surface	NN
cord-311035-s3tkbh9r	62	14	.	.	.
cord-311035-s3tkbh9r	63	1	Following	follow	VBG
cord-311035-s3tkbh9r	63	2	induction	induction	NN
cord-311035-s3tkbh9r	63	3	of	of	IN
cord-311035-s3tkbh9r	63	4	RBD	RBD	NNP
cord-311035-s3tkbh9r	63	5	expression	expression	NN
cord-311035-s3tkbh9r	63	6	,	,	,
cord-311035-s3tkbh9r	63	7	the	the	DT
cord-311035-s3tkbh9r	63	8	yeast	yeast	NN
cord-311035-s3tkbh9r	63	9	were	be	VBD
cord-311035-s3tkbh9r	63	10	incubated	incubate	VBN
cord-311035-s3tkbh9r	63	11	with	with	IN
cord-311035-s3tkbh9r	63	12	dimeric	dimeric	JJ
cord-311035-s3tkbh9r	63	13	,	,	,
cord-311035-s3tkbh9r	63	14	biotinylated	biotinylated	JJ
cord-311035-s3tkbh9r	63	15	sACE2	sACE2	NNP
cord-311035-s3tkbh9r	63	16	(	(	-LRB-
cord-311035-s3tkbh9r	63	17	tan	tan	NNP
cord-311035-s3tkbh9r	63	18	)	)	-RRB-
cord-311035-s3tkbh9r	63	19	.	.	.
cord-311035-s3tkbh9r	64	1	Bound	Bound	NNP
cord-311035-s3tkbh9r	64	2	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	64	3	was	be	VBD
cord-311035-s3tkbh9r	64	4	detected	detect	VBN
cord-311035-s3tkbh9r	64	5	with	with	IN
cord-311035-s3tkbh9r	64	6	fluorescent	fluorescent	JJ
cord-311035-s3tkbh9r	64	7	streptavidin	streptavidin	NNS
cord-311035-s3tkbh9r	64	8	(	(	-LRB-
cord-311035-s3tkbh9r	64	9	purple	purple	NN
cord-311035-s3tkbh9r	64	10	)	)	-RRB-
cord-311035-s3tkbh9r	64	11	and	and	CC
cord-311035-s3tkbh9r	64	12	surface	surface	NN
cord-311035-s3tkbh9r	64	13	expressed	express	VBD
cord-311035-s3tkbh9r	64	14	RBD	RBD	NNP
cord-311035-s3tkbh9r	64	15	was	be	VBD
cord-311035-s3tkbh9r	64	16	detected	detect	VBN
cord-311035-s3tkbh9r	64	17	with	with	IN
cord-311035-s3tkbh9r	64	18	a	a	DT
cord-311035-s3tkbh9r	64	19	fluorescent	fluorescent	JJ
cord-311035-s3tkbh9r	64	20	antibody	antibody	NN
cord-311035-s3tkbh9r	64	21	(	(	-LRB-
cord-311035-s3tkbh9r	64	22	red	red	NNP
cord-311035-s3tkbh9r	64	23	)	)	-RRB-
cord-311035-s3tkbh9r	64	24	.	.	.
cord-311035-s3tkbh9r	65	1	•	•	NNP
cord-311035-s3tkbh9r	66	1	In	in	IN
cord-311035-s3tkbh9r	66	2	deep	deep	JJ
cord-311035-s3tkbh9r	66	3	mutagenesis	mutagenesis	NN
cord-311035-s3tkbh9r	66	4	,	,	,
cord-311035-s3tkbh9r	66	5	the	the	DT
cord-311035-s3tkbh9r	66	6	relative	relative	JJ
cord-311035-s3tkbh9r	66	7	phenotypes	phenotype	NNS
cord-311035-s3tkbh9r	66	8	of	of	IN
cord-311035-s3tkbh9r	66	9	thousands	thousand	NNS
cord-311035-s3tkbh9r	66	10	of	of	IN
cord-311035-s3tkbh9r	66	11	mutations	mutation	NNS
cord-311035-s3tkbh9r	66	12	in	in	IN
cord-311035-s3tkbh9r	66	13	a	a	DT
cord-311035-s3tkbh9r	66	14	protein	protein	NN
cord-311035-s3tkbh9r	66	15	sequence	sequence	NN
cord-311035-s3tkbh9r	66	16	are	be	VBP
cord-311035-s3tkbh9r	66	17	determined	determine	VBN
cord-311035-s3tkbh9r	66	18	in	in	IN
cord-311035-s3tkbh9r	66	19	a	a	DT
cord-311035-s3tkbh9r	66	20	single	single	JJ
cord-311035-s3tkbh9r	66	21	experiment	experiment	NN
cord-311035-s3tkbh9r	66	22	.	.	.
cord-311035-s3tkbh9r	67	1	•	•	NNP
cord-311035-s3tkbh9r	68	1	The	the	DT
cord-311035-s3tkbh9r	68	2	experimental	experimental	JJ
cord-311035-s3tkbh9r	68	3	mutational	mutational	JJ
cord-311035-s3tkbh9r	68	4	landscape	landscape	NN
cord-311035-s3tkbh9r	68	5	of	of	IN
cord-311035-s3tkbh9r	68	6	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	68	7	for	for	IN
cord-311035-s3tkbh9r	68	8	binding	bind	VBG
cord-311035-s3tkbh9r	68	9	the	the	DT
cord-311035-s3tkbh9r	68	10	RBD	RBD	NNP
cord-311035-s3tkbh9r	68	11	of	of	IN
cord-311035-s3tkbh9r	68	12	SARS	SARS	NNP
cord-311035-s3tkbh9r	68	13	-	-	HYPH
cord-311035-s3tkbh9r	68	14	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	68	15	provides	provide	VBZ
cord-311035-s3tkbh9r	68	16	a	a	DT
cord-311035-s3tkbh9r	68	17	blueprint	blueprint	NN
cord-311035-s3tkbh9r	68	18	for	for	IN
cord-311035-s3tkbh9r	68	19	engineering	engineer	VBG
cord-311035-s3tkbh9r	68	20	high	high	JJ
cord-311035-s3tkbh9r	68	21	affinity	affinity	NN
cord-311035-s3tkbh9r	68	22	decoy	decoy	NN
cord-311035-s3tkbh9r	68	23	receptors	receptor	NNS
cord-311035-s3tkbh9r	68	24	.	.	.
cord-311035-s3tkbh9r	69	1	•	•	NNP
cord-311035-s3tkbh9r	70	1	A	a	DT
cord-311035-s3tkbh9r	70	2	deep	deep	JJ
cord-311035-s3tkbh9r	70	3	mutational	mutational	JJ
cord-311035-s3tkbh9r	70	4	scan	scan	NN
cord-311035-s3tkbh9r	70	5	of	of	IN
cord-311035-s3tkbh9r	70	6	the	the	DT
cord-311035-s3tkbh9r	70	7	SARS	SARS	NNP
cord-311035-s3tkbh9r	70	8	-	-	HYPH
cord-311035-s3tkbh9r	70	9	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	70	10	RBD	RBD	NNP
cord-311035-s3tkbh9r	70	11	reveals	reveal	VBZ
cord-311035-s3tkbh9r	70	12	considerable	considerable	JJ
cord-311035-s3tkbh9r	70	13	opportunity	opportunity	NN
cord-311035-s3tkbh9r	70	14	for	for	IN
cord-311035-s3tkbh9r	70	15	genetic	genetic	JJ
cord-311035-s3tkbh9r	70	16	drift	drift	NN
cord-311035-s3tkbh9r	70	17	without	without	IN
cord-311035-s3tkbh9r	70	18	loss	loss	NN
cord-311035-s3tkbh9r	70	19	of	of	IN
cord-311035-s3tkbh9r	70	20	receptor	receptor	NN
cord-311035-s3tkbh9r	70	21	affinity	affinity	NN
cord-311035-s3tkbh9r	70	22	.	.	.
cord-311035-s3tkbh9r	71	1	•	•	NNP
cord-311035-s3tkbh9r	72	1	Different	different	JJ
cord-311035-s3tkbh9r	72	2	expression	expression	NN
cord-311035-s3tkbh9r	72	3	systems	system	NNS
cord-311035-s3tkbh9r	72	4	for	for	IN
cord-311035-s3tkbh9r	72	5	selecting	select	VBG
cord-311035-s3tkbh9r	72	6	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	72	7	or	or	CC
cord-311035-s3tkbh9r	72	8	spike	spike	NN
cord-311035-s3tkbh9r	72	9	variants	variant	NNS
cord-311035-s3tkbh9r	72	10	have	have	VBP
cord-311035-s3tkbh9r	72	11	inherent	inherent	JJ
cord-311035-s3tkbh9r	72	12	advantages	advantage	NNS
cord-311035-s3tkbh9r	72	13	and	and	CC
cord-311035-s3tkbh9r	72	14	disadvantages	disadvantage	NNS
cord-311035-s3tkbh9r	72	15	.	.	.
cord-311035-s3tkbh9r	73	1	•	•	NNP
cord-311035-s3tkbh9r	74	1	There	there	EX
cord-311035-s3tkbh9r	74	2	are	be	VBP
cord-311035-s3tkbh9r	74	3	opportunities	opportunity	NNS
cord-311035-s3tkbh9r	74	4	for	for	IN
cord-311035-s3tkbh9r	74	5	deep	deep	JJ
cord-311035-s3tkbh9r	74	6	mutagenesis	mutagenesis	NN
cord-311035-s3tkbh9r	74	7	to	to	TO
cord-311035-s3tkbh9r	74	8	provide	provide	VB
cord-311035-s3tkbh9r	74	9	biochemical	biochemical	JJ
cord-311035-s3tkbh9r	74	10	insights	insight	NNS
cord-311035-s3tkbh9r	74	11	on	on	IN
cord-311035-s3tkbh9r	74	12	other	other	JJ
cord-311035-s3tkbh9r	74	13	SARS	SARS	NNP
cord-311035-s3tkbh9r	74	14	-	-	HYPH
cord-311035-s3tkbh9r	74	15	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	74	16	proteins	protein	NNS
cord-311035-s3tkbh9r	74	17	.	.	.
cord-311035-s3tkbh9r	75	1	culture	culture	NN
cord-311035-s3tkbh9r	75	2	do	do	VBP
cord-311035-s3tkbh9r	75	3	not	not	RB
cord-311035-s3tkbh9r	75	4	express	express	VB
cord-311035-s3tkbh9r	75	5	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	75	6	and	and	CC
cord-311035-s3tkbh9r	75	7	FACS	FACS	NNP
cord-311035-s3tkbh9r	75	8	time	time	NN
cord-311035-s3tkbh9r	75	9	is	be	VBZ
cord-311035-s3tkbh9r	75	10	wasted	waste	VBN
cord-311035-s3tkbh9r	75	11	on	on	IN
cord-311035-s3tkbh9r	75	12	sorting	sort	VBG
cord-311035-s3tkbh9r	75	13	a	a	DT
cord-311035-s3tkbh9r	75	14	large	large	JJ
cord-311035-s3tkbh9r	75	15	number	number	NN
cord-311035-s3tkbh9r	75	16	of	of	IN
cord-311035-s3tkbh9r	75	17	negative	negative	JJ
cord-311035-s3tkbh9r	75	18	cells	cell	NNS
cord-311035-s3tkbh9r	75	19	.	.	.
cord-311035-s3tkbh9r	76	1	This	this	DT
cord-311035-s3tkbh9r	76	2	has	have	VBZ
cord-311035-s3tkbh9r	76	3	important	important	JJ
cord-311035-s3tkbh9r	76	4	consequences	consequence	NNS
cord-311035-s3tkbh9r	76	5	on	on	IN
cord-311035-s3tkbh9r	76	6	the	the	DT
cord-311035-s3tkbh9r	76	7	data	datum	NNS
cord-311035-s3tkbh9r	76	8	,	,	,
cord-311035-s3tkbh9r	76	9	as	as	IN
cord-311035-s3tkbh9r	76	10	time	time	NN
cord-311035-s3tkbh9r	76	11	spent	spend	VBD
cord-311035-s3tkbh9r	76	12	sampling	sampling	NN
cord-311035-s3tkbh9r	76	13	negative	negative	JJ
cord-311035-s3tkbh9r	76	14	cells	cell	NNS
cord-311035-s3tkbh9r	76	15	is	be	VBZ
cord-311035-s3tkbh9r	76	16	time	time	NN
cord-311035-s3tkbh9r	76	17	not	not	RB
cord-311035-s3tkbh9r	76	18	spent	spend	VBD
cord-311035-s3tkbh9r	76	19	sampling	sampling	NN
cord-311035-s3tkbh9r	76	20	cells	cell	NNS
cord-311035-s3tkbh9r	76	21	expressing	express	VBG
cord-311035-s3tkbh9r	76	22	the	the	DT
cord-311035-s3tkbh9r	76	23	protein	protein	NN
cord-311035-s3tkbh9r	76	24	under	under	IN
cord-311035-s3tkbh9r	76	25	investigation	investigation	NN
cord-311035-s3tkbh9r	76	26	,	,	,
cord-311035-s3tkbh9r	76	27	and	and	CC
cord-311035-s3tkbh9r	76	28	consequently	consequently	RB
cord-311035-s3tkbh9r	76	29	variants	variant	VBZ
cord-311035-s3tkbh9r	76	30	in	in	IN
cord-311035-s3tkbh9r	76	31	the	the	DT
cord-311035-s3tkbh9r	76	32	library	library	NN
cord-311035-s3tkbh9r	76	33	may	may	MD
cord-311035-s3tkbh9r	76	34	be	be	VB
cord-311035-s3tkbh9r	76	35	under	under	RB
cord-311035-s3tkbh9r	76	36	-	-	HYPH
cord-311035-s3tkbh9r	76	37	sampled	sample	VBN
cord-311035-s3tkbh9r	76	38	giving	give	VBG
cord-311035-s3tkbh9r	76	39	poor	poor	JJ
cord-311035-s3tkbh9r	76	40	data	datum	NNS
cord-311035-s3tkbh9r	76	41	accuracy	accuracy	NN
cord-311035-s3tkbh9r	76	42	.	.	.
cord-311035-s3tkbh9r	77	1	Undersampling	Undersampling	NNP
cord-311035-s3tkbh9r	77	2	becomes	become	VBZ
cord-311035-s3tkbh9r	77	3	exceptionally	exceptionally	RB
cord-311035-s3tkbh9r	77	4	concerning	concern	VBG
cord-311035-s3tkbh9r	77	5	as	as	IN
cord-311035-s3tkbh9r	77	6	the	the	DT
cord-311035-s3tkbh9r	77	7	library	library	NN
cord-311035-s3tkbh9r	77	8	size	size	NN
cord-311035-s3tkbh9r	77	9	increases	increase	NNS
cord-311035-s3tkbh9r	77	10	,	,	,
cord-311035-s3tkbh9r	77	11	and	and	CC
cord-311035-s3tkbh9r	77	12	for	for	IN
cord-311035-s3tkbh9r	77	13	this	this	DT
cord-311035-s3tkbh9r	77	14	reason	reason	NN
cord-311035-s3tkbh9r	77	15	the	the	DT
cord-311035-s3tkbh9r	77	16	library	library	NN
cord-311035-s3tkbh9r	77	17	was	be	VBD
cord-311035-s3tkbh9r	77	18	limited	limit	VBN
cord-311035-s3tkbh9r	77	19	to	to	IN
cord-311035-s3tkbh9r	77	20	single	single	JJ
cord-311035-s3tkbh9r	77	21	amino	amino	NN
cord-311035-s3tkbh9r	77	22	acid	acid	NN
cord-311035-s3tkbh9r	77	23	substitutions	substitution	NNS
cord-311035-s3tkbh9r	77	24	at	at	IN
cord-311035-s3tkbh9r	77	25	just	just	RB
cord-311035-s3tkbh9r	77	26	117	117	CD
cord-311035-s3tkbh9r	77	27	positions	position	NNS
cord-311035-s3tkbh9r	77	28	in	in	IN
cord-311035-s3tkbh9r	77	29	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	77	30	.	.	.
cord-311035-s3tkbh9r	78	1	Following	follow	VBG
cord-311035-s3tkbh9r	78	2	FACS	FACS	NNP
cord-311035-s3tkbh9r	78	3	selection	selection	NN
cord-311035-s3tkbh9r	78	4	of	of	IN
cord-311035-s3tkbh9r	78	5	the	the	DT
cord-311035-s3tkbh9r	78	6	human	human	JJ
cord-311035-s3tkbh9r	78	7	culture	culture	NN
cord-311035-s3tkbh9r	78	8	to	to	TO
cord-311035-s3tkbh9r	78	9	enrich	enrich	VB
cord-311035-s3tkbh9r	78	10	a	a	DT
cord-311035-s3tkbh9r	78	11	cell	cell	NN
cord-311035-s3tkbh9r	78	12	population	population	NN
cord-311035-s3tkbh9r	78	13	with	with	IN
cord-311035-s3tkbh9r	78	14	high	high	JJ
cord-311035-s3tkbh9r	78	15	binding	binding	NN
cord-311035-s3tkbh9r	78	16	activity	activity	NN
cord-311035-s3tkbh9r	78	17	for	for	IN
cord-311035-s3tkbh9r	78	18	SARS	SARS	NNP
cord-311035-s3tkbh9r	78	19	-	-	HYPH
cord-311035-s3tkbh9r	78	20	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	78	21	protein	protein	NN
cord-311035-s3tkbh9r	78	22	S	S	NNP
cord-311035-s3tkbh9r	78	23	,	,	,
cord-311035-s3tkbh9r	78	24	RNA	RNA	NNP
cord-311035-s3tkbh9r	78	25	transcripts	transcript	NNS
cord-311035-s3tkbh9r	78	26	were	be	VBD
cord-311035-s3tkbh9r	78	27	isolated	isolate	VBN
cord-311035-s3tkbh9r	78	28	and	and	CC
cord-311035-s3tkbh9r	78	29	Illumina	Illumina	NNP
cord-311035-s3tkbh9r	78	30	sequenced	sequence	VBN
cord-311035-s3tkbh9r	78	31	.	.	.
cord-311035-s3tkbh9r	79	1	An	an	DT
cord-311035-s3tkbh9r	79	2	enrichment	enrichment	NN
cord-311035-s3tkbh9r	79	3	ratio	ratio	NN
cord-311035-s3tkbh9r	79	4	is	be	VBZ
cord-311035-s3tkbh9r	79	5	calculated	calculate	VBN
cord-311035-s3tkbh9r	79	6	for	for	IN
cord-311035-s3tkbh9r	79	7	each	each	DT
cord-311035-s3tkbh9r	79	8	mutation	mutation	NN
cord-311035-s3tkbh9r	79	9	by	by	IN
cord-311035-s3tkbh9r	79	10	dividing	divide	VBG
cord-311035-s3tkbh9r	79	11	its	-PRON-	PRP$
cord-311035-s3tkbh9r	79	12	frequency	frequency	NN
cord-311035-s3tkbh9r	79	13	in	in	IN
cord-311035-s3tkbh9r	79	14	the	the	DT
cord-311035-s3tkbh9r	79	15	sorted	sorted	JJ
cord-311035-s3tkbh9r	79	16	cell	cell	NN
cord-311035-s3tkbh9r	79	17	transcripts	transcript	NNS
cord-311035-s3tkbh9r	79	18	by	by	IN
cord-311035-s3tkbh9r	79	19	its	-PRON-	PRP$
cord-311035-s3tkbh9r	79	20	frequency	frequency	NN
cord-311035-s3tkbh9r	79	21	in	in	IN
cord-311035-s3tkbh9r	79	22	the	the	DT
cord-311035-s3tkbh9r	79	23	naive	naive	JJ
cord-311035-s3tkbh9r	79	24	plasmid	plasmid	NN
cord-311035-s3tkbh9r	79	25	library	library	NN
cord-311035-s3tkbh9r	79	26	[	[	-LRB-
cord-311035-s3tkbh9r	79	27	51	51	CD
cord-311035-s3tkbh9r	79	28	]	]	-RRB-
cord-311035-s3tkbh9r	79	29	.	.	.
cord-311035-s3tkbh9r	80	1	Illumina	Illumina	NNP
cord-311035-s3tkbh9r	80	2	sequencing	sequencing	NN
cord-311035-s3tkbh9r	80	3	did	do	VBD
cord-311035-s3tkbh9r	80	4	not	not	RB
cord-311035-s3tkbh9r	80	5	cover	cover	VB
cord-311035-s3tkbh9r	80	6	the	the	DT
cord-311035-s3tkbh9r	80	7	full	full	JJ
cord-311035-s3tkbh9r	80	8	length	length	NN
cord-311035-s3tkbh9r	80	9	of	of	IN
cord-311035-s3tkbh9r	80	10	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	80	11	and	and	CC
cord-311035-s3tkbh9r	80	12	instead	instead	RB
cord-311035-s3tkbh9r	80	13	the	the	DT
cord-311035-s3tkbh9r	80	14	cDNA	cdna	NN
cord-311035-s3tkbh9r	80	15	was	be	VBD
cord-311035-s3tkbh9r	80	16	sequenced	sequence	VBN
cord-311035-s3tkbh9r	80	17	as	as	IN
cord-311035-s3tkbh9r	80	18	a	a	DT
cord-311035-s3tkbh9r	80	19	series	series	NN
cord-311035-s3tkbh9r	80	20	of	of	IN
cord-311035-s3tkbh9r	80	21	fragments	fragment	NNS
cord-311035-s3tkbh9r	80	22	that	that	WDT
cord-311035-s3tkbh9r	80	23	together	together	RB
cord-311035-s3tkbh9r	80	24	provided	provide	VBD
cord-311035-s3tkbh9r	80	25	full	full	JJ
cord-311035-s3tkbh9r	80	26	coverage	coverage	NN
cord-311035-s3tkbh9r	80	27	of	of	IN
cord-311035-s3tkbh9r	80	28	the	the	DT
cord-311035-s3tkbh9r	80	29	diversified	diversified	JJ
cord-311035-s3tkbh9r	80	30	regions	region	NNS
cord-311035-s3tkbh9r	80	31	.	.	.
cord-311035-s3tkbh9r	81	1	One	one	PRP
cord-311035-s3tkbh9r	81	2	assumes	assume	VBZ
cord-311035-s3tkbh9r	81	3	during	during	IN
cord-311035-s3tkbh9r	81	4	analysis	analysis	NN
cord-311035-s3tkbh9r	81	5	that	that	IN
cord-311035-s3tkbh9r	81	6	there	there	EX
cord-311035-s3tkbh9r	81	7	are	be	VBP
cord-311035-s3tkbh9r	81	8	no	no	DT
cord-311035-s3tkbh9r	81	9	additional	additional	JJ
cord-311035-s3tkbh9r	81	10	mutations	mutation	NNS
cord-311035-s3tkbh9r	81	11	outside	outside	IN
cord-311035-s3tkbh9r	81	12	a	a	DT
cord-311035-s3tkbh9r	81	13	sequenced	sequence	VBN
cord-311035-s3tkbh9r	81	14	fragment	fragment	NN
cord-311035-s3tkbh9r	81	15	,	,	,
cord-311035-s3tkbh9r	81	16	a	a	DT
cord-311035-s3tkbh9r	81	17	reasonable	reasonable	JJ
cord-311035-s3tkbh9r	81	18	assumption	assumption	NN
cord-311035-s3tkbh9r	81	19	when	when	WRB
cord-311035-s3tkbh9r	81	20	a	a	DT
cord-311035-s3tkbh9r	81	21	mutation	mutation	NN
cord-311035-s3tkbh9r	81	22	is	be	VBZ
cord-311035-s3tkbh9r	81	23	found	find	VBN
cord-311035-s3tkbh9r	81	24	because	because	IN
cord-311035-s3tkbh9r	81	25	the	the	DT
cord-311035-s3tkbh9r	81	26	library	library	NN
cord-311035-s3tkbh9r	81	27	was	be	VBD
cord-311035-s3tkbh9r	81	28	constructed	construct	VBN
cord-311035-s3tkbh9r	81	29	to	to	TO
cord-311035-s3tkbh9r	81	30	have	have	VB
cord-311035-s3tkbh9r	81	31	only	only	RB
cord-311035-s3tkbh9r	81	32	one	one	CD
cord-311035-s3tkbh9r	81	33	amino	amino	NN
cord-311035-s3tkbh9r	81	34	acid	acid	NN
cord-311035-s3tkbh9r	81	35	substitution	substitution	NN
cord-311035-s3tkbh9r	81	36	per	per	IN
cord-311035-s3tkbh9r	81	37	plasmid	plasmid	NN
cord-311035-s3tkbh9r	81	38	.	.	.
cord-311035-s3tkbh9r	82	1	However	however	RB
cord-311035-s3tkbh9r	82	2	,	,	,
cord-311035-s3tkbh9r	82	3	the	the	DT
cord-311035-s3tkbh9r	82	4	assumption	assumption	NN
cord-311035-s3tkbh9r	82	5	breaks	break	VBZ
cord-311035-s3tkbh9r	82	6	down	down	RP
cord-311035-s3tkbh9r	82	7	when	when	WRB
cord-311035-s3tkbh9r	82	8	no	no	DT
cord-311035-s3tkbh9r	82	9	mutations	mutation	NNS
cord-311035-s3tkbh9r	82	10	are	be	VBP
cord-311035-s3tkbh9r	82	11	observed	observe	VBN
cord-311035-s3tkbh9r	82	12	in	in	IN
cord-311035-s3tkbh9r	82	13	the	the	DT
cord-311035-s3tkbh9r	82	14	sequenced	sequence	VBN
cord-311035-s3tkbh9r	82	15	fragment	fragment	NN
cord-311035-s3tkbh9r	82	16	,	,	,
cord-311035-s3tkbh9r	82	17	as	as	IN
cord-311035-s3tkbh9r	82	18	one	one	PRP
cord-311035-s3tkbh9r	82	19	can	can	MD
cord-311035-s3tkbh9r	82	20	not	not	RB
cord-311035-s3tkbh9r	82	21	know	know	VB
cord-311035-s3tkbh9r	82	22	whether	whether	IN
cord-311035-s3tkbh9r	82	23	there	there	EX
cord-311035-s3tkbh9r	82	24	was	be	VBD
cord-311035-s3tkbh9r	82	25	a	a	DT
cord-311035-s3tkbh9r	82	26	mutation	mutation	NN
cord-311035-s3tkbh9r	82	27	elsewhere	elsewhere	RB
cord-311035-s3tkbh9r	82	28	outside	outside	IN
cord-311035-s3tkbh9r	82	29	the	the	DT
cord-311035-s3tkbh9r	82	30	sequenced	sequence	VBN
cord-311035-s3tkbh9r	82	31	region	region	NN
cord-311035-s3tkbh9r	82	32	.	.	.
cord-311035-s3tkbh9r	83	1	As	as	IN
cord-311035-s3tkbh9r	83	2	a	a	DT
cord-311035-s3tkbh9r	83	3	consequence	consequence	NN
cord-311035-s3tkbh9r	83	4	,	,	,
cord-311035-s3tkbh9r	83	5	the	the	DT
cord-311035-s3tkbh9r	83	6	wild	wild	JJ
cord-311035-s3tkbh9r	83	7	type	type	NN
cord-311035-s3tkbh9r	83	8	sequence	sequence	NN
cord-311035-s3tkbh9r	83	9	is	be	VBZ
cord-311035-s3tkbh9r	83	10	not	not	RB
cord-311035-s3tkbh9r	83	11	directly	directly	RB
cord-311035-s3tkbh9r	83	12	observed	observe	VBN
cord-311035-s3tkbh9r	83	13	and	and	CC
cord-311035-s3tkbh9r	83	14	is	be	VBZ
cord-311035-s3tkbh9r	83	15	instead	instead	RB
cord-311035-s3tkbh9r	83	16	only	only	RB
cord-311035-s3tkbh9r	83	17	estimated	estimate	VBN
cord-311035-s3tkbh9r	83	18	.	.	.
cord-311035-s3tkbh9r	84	1	There	there	EX
cord-311035-s3tkbh9r	84	2	are	be	VBP
cord-311035-s3tkbh9r	84	3	strategies	strategy	NNS
cord-311035-s3tkbh9r	84	4	using	use	VBG
cord-311035-s3tkbh9r	84	5	the	the	DT
cord-311035-s3tkbh9r	84	6	introduction	introduction	NN
cord-311035-s3tkbh9r	84	7	and	and	CC
cord-311035-s3tkbh9r	84	8	analysis	analysis	NN
cord-311035-s3tkbh9r	84	9	of	of	IN
cord-311035-s3tkbh9r	84	10	silent	silent	JJ
cord-311035-s3tkbh9r	84	11	mutations	mutation	NNS
cord-311035-s3tkbh9r	84	12	that	that	WDT
cord-311035-s3tkbh9r	84	13	can	can	MD
cord-311035-s3tkbh9r	84	14	resolve	resolve	VB
cord-311035-s3tkbh9r	84	15	this	this	DT
cord-311035-s3tkbh9r	84	16	issue	issue	NN
cord-311035-s3tkbh9r	84	17	[	[	-LRB-
cord-311035-s3tkbh9r	84	18	52	52	CD
cord-311035-s3tkbh9r	84	19	]	]	-RRB-
cord-311035-s3tkbh9r	84	20	.	.	.
cord-311035-s3tkbh9r	85	1	Overall	overall	RB
cord-311035-s3tkbh9r	85	2	,	,	,
cord-311035-s3tkbh9r	85	3	there	there	EX
cord-311035-s3tkbh9r	85	4	was	be	VBD
cord-311035-s3tkbh9r	85	5	close	close	JJ
cord-311035-s3tkbh9r	85	6	agreement	agreement	NN
cord-311035-s3tkbh9r	85	7	between	between	IN
cord-311035-s3tkbh9r	85	8	the	the	DT
cord-311035-s3tkbh9r	85	9	mutation	mutation	NN
cord-311035-s3tkbh9r	85	10	enrichment	enrichment	NN
cord-311035-s3tkbh9r	85	11	ratios	ratio	NNS
cord-311035-s3tkbh9r	85	12	from	from	IN
cord-311035-s3tkbh9r	85	13	two	two	CD
cord-311035-s3tkbh9r	85	14	independent	independent	JJ
cord-311035-s3tkbh9r	85	15	replicates	replicate	NNS
cord-311035-s3tkbh9r	85	16	of	of	IN
cord-311035-s3tkbh9r	85	17	the	the	DT
cord-311035-s3tkbh9r	85	18	FACS	FACS	NNP
cord-311035-s3tkbh9r	85	19	experiments	experiment	NNS
cord-311035-s3tkbh9r	85	20	,	,	,
cord-311035-s3tkbh9r	85	21	indicating	indicate	VBG
cord-311035-s3tkbh9r	85	22	that	that	IN
cord-311035-s3tkbh9r	85	23	the	the	DT
cord-311035-s3tkbh9r	85	24	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	85	25	library	library	NN
cord-311035-s3tkbh9r	85	26	was	be	VBD
cord-311035-s3tkbh9r	85	27	well	well	RB
cord-311035-s3tkbh9r	85	28	sampled	sample	VBN
cord-311035-s3tkbh9r	85	29	and	and	CC
cord-311035-s3tkbh9r	85	30	there	there	EX
cord-311035-s3tkbh9r	85	31	was	be	VBD
cord-311035-s3tkbh9r	85	32	high	high	JJ
cord-311035-s3tkbh9r	85	33	confidence	confidence	NN
cord-311035-s3tkbh9r	85	34	in	in	IN
cord-311035-s3tkbh9r	85	35	the	the	DT
cord-311035-s3tkbh9r	85	36	data	datum	NNS
cord-311035-s3tkbh9r	85	37	[	[	-LRB-
cord-311035-s3tkbh9r	85	38	15	15	CD
cord-311035-s3tkbh9r	85	39	]	]	-RRB-
cord-311035-s3tkbh9r	85	40	.	.	.
cord-311035-s3tkbh9r	86	1	The	the	DT
cord-311035-s3tkbh9r	86	2	enrichment	enrichment	NN
cord-311035-s3tkbh9r	86	3	ratios	ratio	NNS
cord-311035-s3tkbh9r	86	4	calculated	calculate	VBN
cord-311035-s3tkbh9r	86	5	for	for	IN
cord-311035-s3tkbh9r	86	6	each	each	DT
cord-311035-s3tkbh9r	86	7	variant	variant	NN
cord-311035-s3tkbh9r	86	8	in	in	IN
cord-311035-s3tkbh9r	86	9	the	the	DT
cord-311035-s3tkbh9r	86	10	sorted	sorted	JJ
cord-311035-s3tkbh9r	86	11	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	86	12	library	library	NN
cord-311035-s3tkbh9r	86	13	provide	provide	VBP
cord-311035-s3tkbh9r	86	14	a	a	DT
cord-311035-s3tkbh9r	86	15	mutational	mutational	JJ
cord-311035-s3tkbh9r	86	16	landscape	landscape	NN
cord-311035-s3tkbh9r	86	17	that	that	WDT
cord-311035-s3tkbh9r	86	18	defines	define	VBZ
cord-311035-s3tkbh9r	86	19	the	the	DT
cord-311035-s3tkbh9r	86	20	relative	relative	JJ
cord-311035-s3tkbh9r	86	21	phenotypes	phenotype	NNS
cord-311035-s3tkbh9r	86	22	of	of	IN
cord-311035-s3tkbh9r	86	23	thousands	thousand	NNS
cord-311035-s3tkbh9r	86	24	of	of	IN
cord-311035-s3tkbh9r	86	25	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	86	26	mutations	mutation	NNS
cord-311035-s3tkbh9r	86	27	for	for	IN
cord-311035-s3tkbh9r	86	28	binding	bind	VBG
cord-311035-s3tkbh9r	86	29	to	to	IN
cord-311035-s3tkbh9r	86	30	SARS	SARS	NNP
cord-311035-s3tkbh9r	86	31	-	-	HYPH
cord-311035-s3tkbh9r	86	32	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	86	33	S	S	NNP
cord-311035-s3tkbh9r	86	34	[	[	-LRB-
cord-311035-s3tkbh9r	86	35	15	15	CD
cord-311035-s3tkbh9r	86	36	]	]	-RRB-
cord-311035-s3tkbh9r	86	37	.	.	.
cord-311035-s3tkbh9r	87	1	The	the	DT
cord-311035-s3tkbh9r	87	2	data	datum	NNS
cord-311035-s3tkbh9r	87	3	in	in	IN
cord-311035-s3tkbh9r	87	4	this	this	DT
cord-311035-s3tkbh9r	87	5	experiment	experiment	NN
cord-311035-s3tkbh9r	87	6	are	be	VBP
cord-311035-s3tkbh9r	87	7	qualitative	qualitative	JJ
cord-311035-s3tkbh9r	87	8	and	and	CC
cord-311035-s3tkbh9r	87	9	it	-PRON-	PRP
cord-311035-s3tkbh9r	87	10	is	be	VBZ
cord-311035-s3tkbh9r	87	11	unclear	unclear	JJ
cord-311035-s3tkbh9r	87	12	how	how	WRB
cord-311035-s3tkbh9r	87	13	a	a	DT
cord-311035-s3tkbh9r	87	14	log	log	NN
cord-311035-s3tkbh9r	87	15	2	2	CD
cord-311035-s3tkbh9r	87	16	enrichment	enrichment	NN
cord-311035-s3tkbh9r	87	17	ratio	ratio	NN
cord-311035-s3tkbh9r	87	18	of	of	IN
cord-311035-s3tkbh9r	87	19	,	,	,
cord-311035-s3tkbh9r	87	20	say	say	UH
cord-311035-s3tkbh9r	87	21	,	,	,
cord-311035-s3tkbh9r	87	22	−2	−2	NNP
cord-311035-s3tkbh9r	87	23	or	or	CC
cord-311035-s3tkbh9r	87	24	+3	+3	NNP
cord-311035-s3tkbh9r	87	25	translates	translate	VBZ
cord-311035-s3tkbh9r	87	26	to	to	IN
cord-311035-s3tkbh9r	87	27	an	an	DT
cord-311035-s3tkbh9r	87	28	exact	exact	JJ
cord-311035-s3tkbh9r	87	29	change	change	NN
cord-311035-s3tkbh9r	87	30	in	in	IN
cord-311035-s3tkbh9r	87	31	a	a	DT
cord-311035-s3tkbh9r	87	32	biophysical	biophysical	JJ
cord-311035-s3tkbh9r	87	33	parameter	parameter	NN
cord-311035-s3tkbh9r	87	34	such	such	JJ
cord-311035-s3tkbh9r	87	35	as	as	IN
cord-311035-s3tkbh9r	87	36	K	K	NNP
cord-311035-s3tkbh9r	87	37	D	D	NNP
cord-311035-s3tkbh9r	87	38	.	.	.
cord-311035-s3tkbh9r	88	1	Furthermore	furthermore	RB
cord-311035-s3tkbh9r	88	2	,	,	,
cord-311035-s3tkbh9r	88	3	mutations	mutation	NNS
cord-311035-s3tkbh9r	88	4	can	can	MD
cord-311035-s3tkbh9r	88	5	impact	impact	VB
cord-311035-s3tkbh9r	88	6	not	not	RB
cord-311035-s3tkbh9r	88	7	only	only	RB
cord-311035-s3tkbh9r	88	8	binding	bind	VBG
cord-311035-s3tkbh9r	88	9	affinity	affinity	NN
cord-311035-s3tkbh9r	88	10	for	for	IN
cord-311035-s3tkbh9r	88	11	the	the	DT
cord-311035-s3tkbh9r	88	12	RBD	RBD	NNP
cord-311035-s3tkbh9r	88	13	of	of	IN
cord-311035-s3tkbh9r	88	14	S	S	NNP
cord-311035-s3tkbh9r	88	15	but	but	CC
cord-311035-s3tkbh9r	88	16	also	also	RB
cord-311035-s3tkbh9r	88	17	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	88	18	surface	surface	NN
cord-311035-s3tkbh9r	88	19	expression	expression	NN
cord-311035-s3tkbh9r	88	20	.	.	.
cord-311035-s3tkbh9r	89	1	To	to	TO
cord-311035-s3tkbh9r	89	2	filter	filter	VB
cord-311035-s3tkbh9r	89	3	out	out	RP
cord-311035-s3tkbh9r	89	4	the	the	DT
cord-311035-s3tkbh9r	89	5	contribution	contribution	NN
cord-311035-s3tkbh9r	89	6	of	of	IN
cord-311035-s3tkbh9r	89	7	mutations	mutation	NNS
cord-311035-s3tkbh9r	89	8	to	to	IN
cord-311035-s3tkbh9r	89	9	expression	expression	NN
cord-311035-s3tkbh9r	89	10	,	,	,
cord-311035-s3tkbh9r	89	11	two	two	CD
cord-311035-s3tkbh9r	89	12	populations	population	NNS
cord-311035-s3tkbh9r	89	13	of	of	IN
cord-311035-s3tkbh9r	89	14	cells	cell	NNS
cord-311035-s3tkbh9r	89	15	were	be	VBD
cord-311035-s3tkbh9r	89	16	collected	collect	VBN
cord-311035-s3tkbh9r	89	17	by	by	IN
cord-311035-s3tkbh9r	89	18	FACS	FACS	NNP
cord-311035-s3tkbh9r	89	19	.	.	.
cord-311035-s3tkbh9r	90	1	In	in	IN
cord-311035-s3tkbh9r	90	2	addition	addition	NN
cord-311035-s3tkbh9r	90	3	to	to	IN
cord-311035-s3tkbh9r	90	4	collecting	collect	VBG
cord-311035-s3tkbh9r	90	5	cells	cell	NNS
cord-311035-s3tkbh9r	90	6	that	that	WDT
cord-311035-s3tkbh9r	90	7	express	express	VBP
cord-311035-s3tkbh9r	90	8	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	90	9	and	and	CC
cord-311035-s3tkbh9r	90	10	tightly	tightly	RB
cord-311035-s3tkbh9r	90	11	bind	bind	VB
cord-311035-s3tkbh9r	90	12	RBD	RBD	NNP
cord-311035-s3tkbh9r	90	13	,	,	,
cord-311035-s3tkbh9r	90	14	cells	cell	NNS
cord-311035-s3tkbh9r	90	15	were	be	VBD
cord-311035-s3tkbh9r	90	16	simultaneously	simultaneously	RB
cord-311035-s3tkbh9r	90	17	collected	collect	VBN
cord-311035-s3tkbh9r	90	18	in	in	IN
cord-311035-s3tkbh9r	90	19	the	the	DT
cord-311035-s3tkbh9r	90	20	same	same	JJ
cord-311035-s3tkbh9r	90	21	experiment	experiment	NN
cord-311035-s3tkbh9r	90	22	that	that	WDT
cord-311035-s3tkbh9r	90	23	express	express	VBP
cord-311035-s3tkbh9r	90	24	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	90	25	but	but	CC
cord-311035-s3tkbh9r	90	26	have	have	VBP
cord-311035-s3tkbh9r	90	27	weak	weak	JJ
cord-311035-s3tkbh9r	90	28	RBD	RBD	NNP
cord-311035-s3tkbh9r	90	29	binding	binding	NN
cord-311035-s3tkbh9r	90	30	.	.	.
cord-311035-s3tkbh9r	91	1	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	91	2	mutants	mutant	NNS
cord-311035-s3tkbh9r	91	3	that	that	WDT
cord-311035-s3tkbh9r	91	4	were	be	VBD
cord-311035-s3tkbh9r	91	5	not	not	RB
cord-311035-s3tkbh9r	91	6	expressed	express	VBN
cord-311035-s3tkbh9r	91	7	at	at	IN
cord-311035-s3tkbh9r	91	8	the	the	DT
cord-311035-s3tkbh9r	91	9	cell	cell	NN
cord-311035-s3tkbh9r	91	10	surface	surface	NN
cord-311035-s3tkbh9r	91	11	would	would	MD
cord-311035-s3tkbh9r	91	12	be	be	VB
cord-311035-s3tkbh9r	91	13	depleted	deplete	VBN
cord-311035-s3tkbh9r	91	14	from	from	IN
cord-311035-s3tkbh9r	91	15	both	both	DT
cord-311035-s3tkbh9r	91	16	sorted	sort	VBN
cord-311035-s3tkbh9r	91	17	populations	population	NNS
cord-311035-s3tkbh9r	91	18	,	,	,
cord-311035-s3tkbh9r	91	19	which	which	WDT
cord-311035-s3tkbh9r	91	20	was	be	VBD
cord-311035-s3tkbh9r	91	21	apparent	apparent	JJ
cord-311035-s3tkbh9r	91	22	from	from	IN
cord-311035-s3tkbh9r	91	23	tracking	track	VBG
cord-311035-s3tkbh9r	91	24	the	the	DT
cord-311035-s3tkbh9r	91	25	depletion	depletion	NN
cord-311035-s3tkbh9r	91	26	of	of	IN
cord-311035-s3tkbh9r	91	27	nonsense	nonsense	NN
cord-311035-s3tkbh9r	91	28	mutations	mutation	NNS
cord-311035-s3tkbh9r	91	29	.	.	.
cord-311035-s3tkbh9r	92	1	In	in	IN
cord-311035-s3tkbh9r	92	2	this	this	DT
cord-311035-s3tkbh9r	92	3	way	way	NN
cord-311035-s3tkbh9r	92	4	,	,	,
cord-311035-s3tkbh9r	92	5	information	information	NN
cord-311035-s3tkbh9r	92	6	was	be	VBD
cord-311035-s3tkbh9r	92	7	collected	collect	VBN
cord-311035-s3tkbh9r	92	8	on	on	IN
cord-311035-s3tkbh9r	92	9	how	how	WRB
cord-311035-s3tkbh9r	92	10	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	92	11	mutations	mutation	NNS
cord-311035-s3tkbh9r	92	12	impact	impact	NN
cord-311035-s3tkbh9r	92	13	expression	expression	NN
cord-311035-s3tkbh9r	92	14	and	and	CC
cord-311035-s3tkbh9r	92	15	RBD	rbd	NN
cord-311035-s3tkbh9r	92	16	binding	bind	VBG
cord-311035-s3tkbh9r	92	17	from	from	IN
cord-311035-s3tkbh9r	92	18	a	a	DT
cord-311035-s3tkbh9r	92	19	single	single	JJ
cord-311035-s3tkbh9r	92	20	FACS	FACS	NNP
cord-311035-s3tkbh9r	92	21	experiment	experiment	NN
cord-311035-s3tkbh9r	92	22	.	.	.
cord-311035-s3tkbh9r	93	1	The	the	DT
cord-311035-s3tkbh9r	93	2	deep	deep	JJ
cord-311035-s3tkbh9r	93	3	mutational	mutational	JJ
cord-311035-s3tkbh9r	93	4	scan	scan	NN
cord-311035-s3tkbh9r	93	5	of	of	IN
cord-311035-s3tkbh9r	93	6	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	93	7	revealed	reveal	VBD
cord-311035-s3tkbh9r	93	8	that	that	IN
cord-311035-s3tkbh9r	93	9	mutations	mutation	NNS
cord-311035-s3tkbh9r	93	10	can	can	MD
cord-311035-s3tkbh9r	93	11	indeed	indeed	RB
cord-311035-s3tkbh9r	93	12	be	be	VB
cord-311035-s3tkbh9r	93	13	found	find	VBN
cord-311035-s3tkbh9r	93	14	to	to	TO
cord-311035-s3tkbh9r	93	15	enhance	enhance	VB
cord-311035-s3tkbh9r	93	16	binding	binding	NN
cord-311035-s3tkbh9r	93	17	toward	toward	IN
cord-311035-s3tkbh9r	93	18	SARS	SARS	NNP
cord-311035-s3tkbh9r	93	19	-	-	HYPH
cord-311035-s3tkbh9r	93	20	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	93	21	RBD	RBD	NNP
cord-311035-s3tkbh9r	93	22	(	(	-LRB-
cord-311035-s3tkbh9r	93	23	Figure	figure	NN
cord-311035-s3tkbh9r	93	24	2	2	CD
cord-311035-s3tkbh9r	93	25	)	)	-RRB-
cord-311035-s3tkbh9r	93	26	,	,	,
cord-311035-s3tkbh9r	93	27	suitable	suitable	JJ
cord-311035-s3tkbh9r	93	28	for	for	IN
cord-311035-s3tkbh9r	93	29	engineering	engineer	VBG
cord-311035-s3tkbh9r	93	30	high	high	JJ
cord-311035-s3tkbh9r	93	31	affinity	affinity	NN
cord-311035-s3tkbh9r	93	32	soluble	soluble	JJ
cord-311035-s3tkbh9r	93	33	decoy	decoy	NN
cord-311035-s3tkbh9r	93	34	receptors	receptor	NNS
cord-311035-s3tkbh9r	93	35	[	[	-LRB-
cord-311035-s3tkbh9r	93	36	15	15	CD
cord-311035-s3tkbh9r	93	37	]	]	-RRB-
cord-311035-s3tkbh9r	93	38	.	.	.
cord-311035-s3tkbh9r	94	1	Mutations	mutation	NNS
cord-311035-s3tkbh9r	94	2	were	be	VBD
cord-311035-s3tkbh9r	94	3	found	find	VBN
cord-311035-s3tkbh9r	94	4	at	at	IN
cord-311035-s3tkbh9r	94	5	the	the	DT
cord-311035-s3tkbh9r	94	6	binding	bind	VBG
cord-311035-s3tkbh9r	94	7	interface	interface	NN
cord-311035-s3tkbh9r	94	8	where	where	WRB
cord-311035-s3tkbh9r	94	9	they	-PRON-	PRP
cord-311035-s3tkbh9r	94	10	enhance	enhance	VBP
cord-311035-s3tkbh9r	94	11	specific	specific	JJ
cord-311035-s3tkbh9r	94	12	atomic	atomic	JJ
cord-311035-s3tkbh9r	94	13	contacts	contact	NNS
cord-311035-s3tkbh9r	94	14	,	,	,
cord-311035-s3tkbh9r	94	15	and	and	CC
cord-311035-s3tkbh9r	94	16	were	be	VBD
cord-311035-s3tkbh9r	94	17	also	also	RB
cord-311035-s3tkbh9r	94	18	found	find	VBN
cord-311035-s3tkbh9r	94	19	distally	distally	RB
cord-311035-s3tkbh9r	94	20	in	in	IN
cord-311035-s3tkbh9r	94	21	the	the	DT
cord-311035-s3tkbh9r	94	22	second	second	JJ
cord-311035-s3tkbh9r	94	23	shell	shell	NN
cord-311035-s3tkbh9r	94	24	and	and	CC
cord-311035-s3tkbh9r	94	25	beyond	beyond	IN
cord-311035-s3tkbh9r	94	26	where	where	WRB
cord-311035-s3tkbh9r	94	27	they	-PRON-	PRP
cord-311035-s3tkbh9r	94	28	may	may	MD
cord-311035-s3tkbh9r	94	29	impact	impact	VB
cord-311035-s3tkbh9r	94	30	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	94	31	conformation	conformation	NN
cord-311035-s3tkbh9r	94	32	,	,	,
cord-311035-s3tkbh9r	94	33	folding	folding	NN
cord-311035-s3tkbh9r	94	34	and	and	CC
cord-311035-s3tkbh9r	94	35	dynamics	dynamic	NNS
cord-311035-s3tkbh9r	94	36	.	.	.
cord-311035-s3tkbh9r	95	1	A	a	DT
cord-311035-s3tkbh9r	95	2	soluble	soluble	JJ
cord-311035-s3tkbh9r	95	3	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	95	4	variant	variant	NN
cord-311035-s3tkbh9r	95	5	that	that	WDT
cord-311035-s3tkbh9r	95	6	combines	combine	VBZ
cord-311035-s3tkbh9r	95	7	three	three	CD
cord-311035-s3tkbh9r	95	8	mutations	mutation	NNS
cord-311035-s3tkbh9r	95	9	,	,	,
cord-311035-s3tkbh9r	95	10	called	call	VBD
cord-311035-s3tkbh9r	95	11	sACE2	sACE2	NNP
cord-311035-s3tkbh9r	95	12	2	2	CD
cord-311035-s3tkbh9r	95	13	.v2.4	.v2.4	NFP
cord-311035-s3tkbh9r	95	14	,	,	,
cord-311035-s3tkbh9r	95	15	was	be	VBD
cord-311035-s3tkbh9r	95	16	found	find	VBN
cord-311035-s3tkbh9r	95	17	to	to	TO
cord-311035-s3tkbh9r	95	18	be	be	VB
cord-311035-s3tkbh9r	95	19	highly	highly	RB
cord-311035-s3tkbh9r	95	20	expressed	express	VBN
cord-311035-s3tkbh9r	95	21	,	,	,
cord-311035-s3tkbh9r	95	22	is	be	VBZ
cord-311035-s3tkbh9r	95	23	a	a	DT
cord-311035-s3tkbh9r	95	24	stable	stable	JJ
cord-311035-s3tkbh9r	95	25	monodisperse	monodisperse	NNP
cord-311035-s3tkbh9r	95	26	dimer	dimer	NN
cord-311035-s3tkbh9r	95	27	,	,	,
cord-311035-s3tkbh9r	95	28	binds	bind	VBZ
cord-311035-s3tkbh9r	95	29	SARS	SARS	NNP
cord-311035-s3tkbh9r	95	30	-	-	HYPH
cord-311035-s3tkbh9r	95	31	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	95	32	S	S	NNP
cord-311035-s3tkbh9r	95	33	with	with	IN
cord-311035-s3tkbh9r	95	34	picomolar	picomolar	JJ
cord-311035-s3tkbh9r	95	35	affinity	affinity	NN
cord-311035-s3tkbh9r	95	36	and	and	CC
cord-311035-s3tkbh9r	95	37	potently	potently	RB
cord-311035-s3tkbh9r	95	38	neutralizes	neutralize	VBZ
cord-311035-s3tkbh9r	95	39	infection	infection	NN
cord-311035-s3tkbh9r	95	40	of	of	IN
cord-311035-s3tkbh9r	95	41	a	a	DT
cord-311035-s3tkbh9r	95	42	susceptible	susceptible	JJ
cord-311035-s3tkbh9r	95	43	cell	cell	NN
cord-311035-s3tkbh9r	95	44	line	line	NN
cord-311035-s3tkbh9r	95	45	by	by	IN
cord-311035-s3tkbh9r	95	46	authentic	authentic	JJ
cord-311035-s3tkbh9r	95	47	virus	virus	NN
cord-311035-s3tkbh9r	95	48	.	.	.
cord-311035-s3tkbh9r	96	1	Its	-PRON-	PRP$
cord-311035-s3tkbh9r	96	2	properties	property	NNS
cord-311035-s3tkbh9r	96	3	rival	rival	JJ
cord-311035-s3tkbh9r	96	4	affinity	affinity	NN
cord-311035-s3tkbh9r	96	5	-	-	HYPH
cord-311035-s3tkbh9r	96	6	matured	mature	VBN
cord-311035-s3tkbh9r	96	7	monoclonal	monoclonal	JJ
cord-311035-s3tkbh9r	96	8	antibodies	antibody	NNS
cord-311035-s3tkbh9r	96	9	under	under	IN
cord-311035-s3tkbh9r	96	10	commercial	commercial	JJ
cord-311035-s3tkbh9r	96	11	development	development	NN
cord-311035-s3tkbh9r	96	12	for	for	IN
cord-311035-s3tkbh9r	96	13	therapy	therapy	NN
cord-311035-s3tkbh9r	96	14	and	and	CC
cord-311035-s3tkbh9r	96	15	prophylaxis	prophylaxis	NN
cord-311035-s3tkbh9r	96	16	.	.	.
cord-311035-s3tkbh9r	97	1	Despite	despite	IN
cord-311035-s3tkbh9r	97	2	only	only	JJ
cord-311035-s3tkbh9r	97	3	affinity	affinity	NN
cord-311035-s3tkbh9r	97	4	toward	toward	IN
cord-311035-s3tkbh9r	97	5	SARS	SARS	NNP
cord-311035-s3tkbh9r	97	6	-	-	HYPH
cord-311035-s3tkbh9r	97	7	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	97	8	being	be	VBG
cord-311035-s3tkbh9r	97	9	considered	consider	VBN
cord-311035-s3tkbh9r	97	10	during	during	IN
cord-311035-s3tkbh9r	97	11	the	the	DT
cord-311035-s3tkbh9r	97	12	engineering	engineering	NN
cord-311035-s3tkbh9r	97	13	process	process	NN
cord-311035-s3tkbh9r	97	14	,	,	,
cord-311035-s3tkbh9r	97	15	sACE2	sACE2	NNP
cord-311035-s3tkbh9r	97	16	2	2	CD
cord-311035-s3tkbh9r	97	17	.	.	.
cord-311035-s3tkbh9r	97	18	v2.4	v2.4	NNP
cord-311035-s3tkbh9r	97	19	also	also	RB
cord-311035-s3tkbh9r	97	20	potently	potently	RB
cord-311035-s3tkbh9r	97	21	neutralizes	neutralize	VBZ
cord-311035-s3tkbh9r	97	22	authentic	authentic	JJ
cord-311035-s3tkbh9r	97	23	SARS	SARS	NNP
cord-311035-s3tkbh9r	97	24	-	-	HYPH
cord-311035-s3tkbh9r	97	25	CoV-1	CoV-1	NNP
cord-311035-s3tkbh9r	97	26	,	,	,
cord-311035-s3tkbh9r	97	27	and	and	CC
cord-311035-s3tkbh9r	97	28	we	-PRON-	PRP
cord-311035-s3tkbh9r	97	29	speculate	speculate	VBP
cord-311035-s3tkbh9r	97	30	that	that	IN
cord-311035-s3tkbh9r	97	31	it	-PRON-	PRP
cord-311035-s3tkbh9r	97	32	will	will	MD
cord-311035-s3tkbh9r	97	33	have	have	VB
cord-311035-s3tkbh9r	97	34	broad	broad	JJ
cord-311035-s3tkbh9r	97	35	activity	activity	NN
cord-311035-s3tkbh9r	97	36	against	against	IN
cord-311035-s3tkbh9r	97	37	betacoronaviruses	betacoronaviruse	NNS
cord-311035-s3tkbh9r	97	38	that	that	WDT
cord-311035-s3tkbh9r	97	39	use	use	VBP
cord-311035-s3tkbh9r	97	40	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	97	41	as	as	IN
cord-311035-s3tkbh9r	97	42	an	an	DT
cord-311035-s3tkbh9r	97	43	entry	entry	NN
cord-311035-s3tkbh9r	97	44	receptor	receptor	NN
cord-311035-s3tkbh9r	97	45	.	.	.
cord-311035-s3tkbh9r	98	1	In	in	IN
cord-311035-s3tkbh9r	98	2	unpublished	unpublished	JJ
cord-311035-s3tkbh9r	98	3	work	work	NN
cord-311035-s3tkbh9r	98	4	that	that	WDT
cord-311035-s3tkbh9r	98	5	has	have	VBZ
cord-311035-s3tkbh9r	98	6	yet	yet	RB
cord-311035-s3tkbh9r	98	7	to	to	TO
cord-311035-s3tkbh9r	98	8	be	be	VB
cord-311035-s3tkbh9r	98	9	peer	peer	NN
cord-311035-s3tkbh9r	98	10	reviewed	review	VBN
cord-311035-s3tkbh9r	98	11	,	,	,
cord-311035-s3tkbh9r	98	12	we	-PRON-	PRP
cord-311035-s3tkbh9r	98	13	have	have	VBP
cord-311035-s3tkbh9r	98	14	found	find	VBN
cord-311035-s3tkbh9r	98	15	sACE2	sACE2	NNP
cord-311035-s3tkbh9r	98	16	2	2	CD
cord-311035-s3tkbh9r	98	17	.	.	.
cord-311035-s3tkbh9r	99	1	v2.4	v2.4	NNP
cord-311035-s3tkbh9r	99	2	broadly	broadly	RB
cord-311035-s3tkbh9r	99	3	and	and	CC
cord-311035-s3tkbh9r	99	4	tightly	tightly	RB
cord-311035-s3tkbh9r	99	5	binds	bind	VBZ
cord-311035-s3tkbh9r	99	6	bat	bat	NN
cord-311035-s3tkbh9r	99	7	coronaviruses	coronaviruse	NNS
cord-311035-s3tkbh9r	99	8	that	that	WDT
cord-311035-s3tkbh9r	99	9	may	may	MD
cord-311035-s3tkbh9r	99	10	be	be	VB
cord-311035-s3tkbh9r	99	11	a	a	DT
cord-311035-s3tkbh9r	99	12	source	source	NN
cord-311035-s3tkbh9r	99	13	for	for	IN
cord-311035-s3tkbh9r	99	14	future	future	JJ
cord-311035-s3tkbh9r	99	15	pandemics	pandemic	NNS
cord-311035-s3tkbh9r	99	16	,	,	,
cord-311035-s3tkbh9r	99	17	supporting	support	VBG
cord-311035-s3tkbh9r	99	18	the	the	DT
cord-311035-s3tkbh9r	99	19	concept	concept	NN
cord-311035-s3tkbh9r	99	20	of	of	IN
cord-311035-s3tkbh9r	99	21	receptor	receptor	NN
cord-311035-s3tkbh9r	99	22	-	-	HYPH
cord-311035-s3tkbh9r	99	23	based	base	VBN
cord-311035-s3tkbh9r	99	24	decoys	decoy	NNS
cord-311035-s3tkbh9r	99	25	as	as	IN
cord-311035-s3tkbh9r	99	26	antiviral	antiviral	JJ
cord-311035-s3tkbh9r	99	27	biologics	biologic	NNS
cord-311035-s3tkbh9r	99	28	with	with	IN
cord-311035-s3tkbh9r	99	29	exceptional	exceptional	JJ
cord-311035-s3tkbh9r	99	30	breadth	breadth	NN
cord-311035-s3tkbh9r	99	31	.	.	.
cord-311035-s3tkbh9r	100	1	As	as	IN
cord-311035-s3tkbh9r	100	2	determined	determine	VBN
cord-311035-s3tkbh9r	100	3	by	by	IN
cord-311035-s3tkbh9r	100	4	yeast	yeast	NN
cord-311035-s3tkbh9r	100	5	display	display	NN
cord-311035-s3tkbh9r	100	6	,	,	,
cord-311035-s3tkbh9r	100	7	the	the	DT
cord-311035-s3tkbh9r	100	8	effects	effect	NNS
cord-311035-s3tkbh9r	100	9	of	of	IN
cord-311035-s3tkbh9r	100	10	mutations	mutation	NNS
cord-311035-s3tkbh9r	100	11	in	in	IN
cord-311035-s3tkbh9r	100	12	the	the	DT
cord-311035-s3tkbh9r	100	13	RBD	RBD	NNP
cord-311035-s3tkbh9r	100	14	of	of	IN
cord-311035-s3tkbh9r	100	15	SARS	SARS	NNP
cord-311035-s3tkbh9r	100	16	-	-	HYPH
cord-311035-s3tkbh9r	100	17	COV-2	COV-2	NNP
cord-311035-s3tkbh9r	100	18	protein	protein	NN
cord-311035-s3tkbh9r	100	19	S	S	NNP
cord-311035-s3tkbh9r	100	20	on	on	IN
cord-311035-s3tkbh9r	100	21	receptor	receptor	NN
cord-311035-s3tkbh9r	100	22	affinity	affinity	NN
cord-311035-s3tkbh9r	100	23	are	be	VBP
cord-311035-s3tkbh9r	100	24	plotted	plot	VBN
cord-311035-s3tkbh9r	100	25	in	in	IN
cord-311035-s3tkbh9r	100	26	the	the	DT
cord-311035-s3tkbh9r	100	27	heat	heat	NN
cord-311035-s3tkbh9r	100	28	map	map	NN
cord-311035-s3tkbh9r	100	29	at	at	IN
cord-311035-s3tkbh9r	100	30	left	left	NN
cord-311035-s3tkbh9r	100	31	,	,	,
cord-311035-s3tkbh9r	100	32	with	with	IN
cord-311035-s3tkbh9r	100	33	dark	dark	JJ
cord-311035-s3tkbh9r	100	34	green	green	JJ
cord-311035-s3tkbh9r	100	35	indicating	indicate	VBG
cord-311035-s3tkbh9r	100	36	the	the	DT
cord-311035-s3tkbh9r	100	37	mutations	mutation	NNS
cord-311035-s3tkbh9r	100	38	are	be	VBP
cord-311035-s3tkbh9r	100	39	deleterious	deleterious	JJ
cord-311035-s3tkbh9r	100	40	and	and	CC
cord-311035-s3tkbh9r	100	41	pale	pale	JJ
cord-311035-s3tkbh9r	100	42	colors	color	NNS
cord-311035-s3tkbh9r	100	43	indicating	indicate	VBG
cord-311035-s3tkbh9r	100	44	the	the	DT
cord-311035-s3tkbh9r	100	45	mutations	mutation	NNS
cord-311035-s3tkbh9r	100	46	are	be	VBP
cord-311035-s3tkbh9r	100	47	neutral	neutral	JJ
cord-311035-s3tkbh9r	100	48	.	.	.
cord-311035-s3tkbh9r	101	1	The	the	DT
cord-311035-s3tkbh9r	101	2	effects	effect	NNS
cord-311035-s3tkbh9r	101	3	of	of	IN
cord-311035-s3tkbh9r	101	4	mutations	mutation	NNS
cord-311035-s3tkbh9r	101	5	in	in	IN
cord-311035-s3tkbh9r	101	6	human	human	JJ
cord-311035-s3tkbh9r	101	7	cell	cell	NN
cord-311035-s3tkbh9r	101	8	-	-	:
cord-311035-s3tkbh9r	101	9	expressed	express	VBN
cord-311035-s3tkbh9r	101	10	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	101	11	on	on	IN
cord-311035-s3tkbh9r	101	12	binding	bind	VBG
cord-311035-s3tkbh9r	101	13	to	to	IN
cord-311035-s3tkbh9r	101	14	soluble	soluble	JJ
cord-311035-s3tkbh9r	101	15	RBD	RBD	NNP
cord-311035-s3tkbh9r	101	16	are	be	VBP
cord-311035-s3tkbh9r	101	17	plotted	plot	VBN
cord-311035-s3tkbh9r	101	18	in	in	IN
cord-311035-s3tkbh9r	101	19	the	the	DT
cord-311035-s3tkbh9r	101	20	heat	heat	NN
cord-311035-s3tkbh9r	101	21	map	map	NN
cord-311035-s3tkbh9r	101	22	at	at	IN
cord-311035-s3tkbh9r	101	23	right	right	NN
cord-311035-s3tkbh9r	101	24	,	,	,
cord-311035-s3tkbh9r	101	25	with	with	IN
cord-311035-s3tkbh9r	101	26	depleted	depleted	JJ
cord-311035-s3tkbh9r	101	27	mutations	mutation	NNS
cord-311035-s3tkbh9r	101	28	in	in	IN
cord-311035-s3tkbh9r	101	29	orange	orange	JJ
cord-311035-s3tkbh9r	101	30	,	,	,
cord-311035-s3tkbh9r	101	31	neutral	neutral	JJ
cord-311035-s3tkbh9r	101	32	mutations	mutation	NNS
cord-311035-s3tkbh9r	101	33	in	in	IN
cord-311035-s3tkbh9r	101	34	white	white	JJ
cord-311035-s3tkbh9r	101	35	and	and	CC
cord-311035-s3tkbh9r	101	36	enriched	enriched	JJ
cord-311035-s3tkbh9r	101	37	mutations	mutation	NNS
cord-311035-s3tkbh9r	101	38	in	in	IN
cord-311035-s3tkbh9r	101	39	blue	blue	NN
cord-311035-s3tkbh9r	101	40	.	.	.
cord-311035-s3tkbh9r	102	1	Positional	positional	JJ
cord-311035-s3tkbh9r	102	2	scores	score	NNS
cord-311035-s3tkbh9r	102	3	are	be	VBP
cord-311035-s3tkbh9r	102	4	mapped	map	VBN
cord-311035-s3tkbh9r	102	5	to	to	IN
cord-311035-s3tkbh9r	102	6	the	the	DT
cord-311035-s3tkbh9r	102	7	atomic	atomic	JJ
cord-311035-s3tkbh9r	102	8	structure	structure	NN
cord-311035-s3tkbh9r	102	9	of	of	IN
cord-311035-s3tkbh9r	102	10	RBD	RBD	NNP
cord-311035-s3tkbh9r	102	11	-	-	HYPH
cord-311035-s3tkbh9r	102	12	bound	bind	VBN
cord-311035-s3tkbh9r	102	13	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	102	14	(	(	-LRB-
cord-311035-s3tkbh9r	102	15	PDB	PDB	NNP
cord-311035-s3tkbh9r	102	16	6M17	6m17	CD
cord-311035-s3tkbh9r	102	17	)	)	-RRB-
cord-311035-s3tkbh9r	102	18	at	at	IN
cord-311035-s3tkbh9r	102	19	center	center	NN
cord-311035-s3tkbh9r	102	20	.	.	.
cord-311035-s3tkbh9r	103	1	Conserved	conserved	JJ
cord-311035-s3tkbh9r	103	2	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	103	3	residues	residue	NNS
cord-311035-s3tkbh9r	103	4	for	for	IN
cord-311035-s3tkbh9r	103	5	RBD	RBD	NNP
cord-311035-s3tkbh9r	103	6	binding	binding	NN
cord-311035-s3tkbh9r	103	7	are	be	VBP
cord-311035-s3tkbh9r	103	8	orange	orange	JJ
cord-311035-s3tkbh9r	103	9	,	,	,
cord-311035-s3tkbh9r	103	10	while	while	IN
cord-311035-s3tkbh9r	103	11	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	103	12	residues	residue	NNS
cord-311035-s3tkbh9r	103	13	that	that	WDT
cord-311035-s3tkbh9r	103	14	are	be	VBP
cord-311035-s3tkbh9r	103	15	hot	hot	JJ
cord-311035-s3tkbh9r	103	16	spots	spot	NNS
cord-311035-s3tkbh9r	103	17	for	for	IN
cord-311035-s3tkbh9r	103	18	mutations	mutation	NNS
cord-311035-s3tkbh9r	103	19	with	with	IN
cord-311035-s3tkbh9r	103	20	increased	increase	VBN
cord-311035-s3tkbh9r	103	21	affinity	affinity	NN
cord-311035-s3tkbh9r	103	22	are	be	VBP
cord-311035-s3tkbh9r	103	23	blue	blue	JJ
cord-311035-s3tkbh9r	103	24	.	.	.
cord-311035-s3tkbh9r	104	1	RBD	RBD	NNP
cord-311035-s3tkbh9r	104	2	residues	residue	NNS
cord-311035-s3tkbh9r	104	3	conserved	conserve	VBN
cord-311035-s3tkbh9r	104	4	for	for	IN
cord-311035-s3tkbh9r	104	5	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	104	6	binding	binding	NN
cord-311035-s3tkbh9r	104	7	are	be	VBP
cord-311035-s3tkbh9r	104	8	green	green	JJ
cord-311035-s3tkbh9r	104	9	.	.	.
cord-311035-s3tkbh9r	105	1	Most	Most	JJS
cord-311035-s3tkbh9r	105	2	RBD	RBD	NNP
cord-311035-s3tkbh9r	105	3	mutations	mutation	NNS
cord-311035-s3tkbh9r	105	4	in	in	IN
cord-311035-s3tkbh9r	105	5	this	this	DT
cord-311035-s3tkbh9r	105	6	region	region	NN
cord-311035-s3tkbh9r	105	7	of	of	IN
cord-311035-s3tkbh9r	105	8	the	the	DT
cord-311035-s3tkbh9r	105	9	interface	interface	NN
cord-311035-s3tkbh9r	105	10	are	be	VBP
cord-311035-s3tkbh9r	105	11	deleterious	deleterious	JJ
cord-311035-s3tkbh9r	105	12	,	,	,
cord-311035-s3tkbh9r	105	13	whereas	whereas	IN
cord-311035-s3tkbh9r	105	14	numerous	numerous	JJ
cord-311035-s3tkbh9r	105	15	mutations	mutation	NNS
cord-311035-s3tkbh9r	105	16	were	be	VBD
cord-311035-s3tkbh9r	105	17	found	find	VBN
cord-311035-s3tkbh9r	105	18	in	in	IN
cord-311035-s3tkbh9r	105	19	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	105	20	that	that	WDT
cord-311035-s3tkbh9r	105	21	increased	increase	VBD
cord-311035-s3tkbh9r	105	22	affinity	affinity	NN
cord-311035-s3tkbh9r	105	23	.	.	.
cord-311035-s3tkbh9r	106	1	In	in	IN
cord-311035-s3tkbh9r	106	2	Starr	Starr	NNP
cord-311035-s3tkbh9r	106	3	et	et	NNP
cord-311035-s3tkbh9r	106	4	al	al	NNP
cord-311035-s3tkbh9r	106	5	,	,	,
cord-311035-s3tkbh9r	106	6	deep	deep	JJ
cord-311035-s3tkbh9r	106	7	mutagenesis	mutagenesis	NN
cord-311035-s3tkbh9r	106	8	was	be	VBD
cord-311035-s3tkbh9r	106	9	applied	apply	VBN
cord-311035-s3tkbh9r	106	10	to	to	IN
cord-311035-s3tkbh9r	106	11	the	the	DT
cord-311035-s3tkbh9r	106	12	SARS	SARS	NNP
cord-311035-s3tkbh9r	106	13	-	-	HYPH
cord-311035-s3tkbh9r	106	14	CoV	CoV	NNP
cord-311035-s3tkbh9r	106	15	-2	-2	NN
cord-311035-s3tkbh9r	106	16	spike	spike	NN
cord-311035-s3tkbh9r	106	17	to	to	TO
cord-311035-s3tkbh9r	106	18	assess	assess	VB
cord-311035-s3tkbh9r	106	19	mutational	mutational	JJ
cord-311035-s3tkbh9r	106	20	tolerance	tolerance	NN
cord-311035-s3tkbh9r	106	21	for	for	IN
cord-311035-s3tkbh9r	106	22	expression	expression	NN
cord-311035-s3tkbh9r	106	23	and	and	CC
cord-311035-s3tkbh9r	106	24	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	106	25	interactions	interaction	NNS
cord-311035-s3tkbh9r	106	26	[	[	-LRB-
cord-311035-s3tkbh9r	106	27	14	14	CD
cord-311035-s3tkbh9r	106	28	]	]	-RRB-
cord-311035-s3tkbh9r	106	29	.	.	.
cord-311035-s3tkbh9r	107	1	Instead	instead	RB
cord-311035-s3tkbh9r	107	2	of	of	IN
cord-311035-s3tkbh9r	107	3	investigating	investigate	VBG
cord-311035-s3tkbh9r	107	4	the	the	DT
cord-311035-s3tkbh9r	107	5	entire	entire	JJ
cord-311035-s3tkbh9r	107	6	trimeric	trimeric	NNP
cord-311035-s3tkbh9r	107	7	S	S	NNP
cord-311035-s3tkbh9r	107	8	protein	protein	NN
cord-311035-s3tkbh9r	107	9	expressed	express	VBD
cord-311035-s3tkbh9r	107	10	on	on	IN
cord-311035-s3tkbh9r	107	11	a	a	DT
cord-311035-s3tkbh9r	107	12	cellular	cellular	JJ
cord-311035-s3tkbh9r	107	13	or	or	CC
cord-311035-s3tkbh9r	107	14	viral	viral	JJ
cord-311035-s3tkbh9r	107	15	membrane	membrane	NN
cord-311035-s3tkbh9r	107	16	,	,	,
cord-311035-s3tkbh9r	107	17	the	the	DT
cord-311035-s3tkbh9r	107	18	isolated	isolated	JJ
cord-311035-s3tkbh9r	107	19	RBD	RBD	NNP
cord-311035-s3tkbh9r	107	20	was	be	VBD
cord-311035-s3tkbh9r	107	21	fused	fuse	VBN
cord-311035-s3tkbh9r	107	22	to	to	IN
cord-311035-s3tkbh9r	107	23	the	the	DT
cord-311035-s3tkbh9r	107	24	yeast	yeast	NN
cord-311035-s3tkbh9r	107	25	mating	mating	NN
cord-311035-s3tkbh9r	107	26	factor	factor	NN
cord-311035-s3tkbh9r	107	27	Aga2p	Aga2p	NNP
cord-311035-s3tkbh9r	107	28	and	and	CC
cord-311035-s3tkbh9r	107	29	displayed	display	VBD
cord-311035-s3tkbh9r	107	30	on	on	IN
cord-311035-s3tkbh9r	107	31	the	the	DT
cord-311035-s3tkbh9r	107	32	yeast	yeast	NN
cord-311035-s3tkbh9r	107	33	surface	surface	NN
cord-311035-s3tkbh9r	107	34	[	[	-LRB-
cord-311035-s3tkbh9r	107	35	53	53	CD
cord-311035-s3tkbh9r	107	36	]	]	-RRB-
cord-311035-s3tkbh9r	107	37	(	(	-LRB-
cord-311035-s3tkbh9r	107	38	Figure	figure	NN
cord-311035-s3tkbh9r	107	39	1B	1b	CD
cord-311035-s3tkbh9r	107	40	)	)	-RRB-
cord-311035-s3tkbh9r	107	41	.	.	.
cord-311035-s3tkbh9r	108	1	This	this	DT
cord-311035-s3tkbh9r	108	2	is	be	VBZ
cord-311035-s3tkbh9r	108	3	an	an	DT
cord-311035-s3tkbh9r	108	4	artificial	artificial	JJ
cord-311035-s3tkbh9r	108	5	display	display	NN
cord-311035-s3tkbh9r	108	6	platform	platform	NN
cord-311035-s3tkbh9r	108	7	that	that	WDT
cord-311035-s3tkbh9r	108	8	removes	remove	VBZ
cord-311035-s3tkbh9r	108	9	the	the	DT
cord-311035-s3tkbh9r	108	10	RBD	RBD	NNP
cord-311035-s3tkbh9r	108	11	from	from	IN
cord-311035-s3tkbh9r	108	12	its	-PRON-	PRP$
cord-311035-s3tkbh9r	108	13	native	native	JJ
cord-311035-s3tkbh9r	108	14	context	context	NN
cord-311035-s3tkbh9r	108	15	.	.	.
cord-311035-s3tkbh9r	109	1	N	N	NNP
cord-311035-s3tkbh9r	109	2	-	-	HYPH
cord-311035-s3tkbh9r	109	3	Glycosylation	Glycosylation	NNP
cord-311035-s3tkbh9r	109	4	in	in	IN
cord-311035-s3tkbh9r	109	5	yeast	yeast	NN
cord-311035-s3tkbh9r	109	6	is	be	VBZ
cord-311035-s3tkbh9r	109	7	also	also	RB
cord-311035-s3tkbh9r	109	8	of	of	IN
cord-311035-s3tkbh9r	109	9	high	high	JJ
cord-311035-s3tkbh9r	109	10	-	-	HYPH
cord-311035-s3tkbh9r	109	11	mannose	mannose	NN
cord-311035-s3tkbh9r	109	12	type	type	NN
cord-311035-s3tkbh9r	109	13	and	and	CC
cord-311035-s3tkbh9r	109	14	lacks	lack	VBZ
cord-311035-s3tkbh9r	109	15	the	the	DT
cord-311035-s3tkbh9r	109	16	complex	complex	JJ
cord-311035-s3tkbh9r	109	17	,	,	,
cord-311035-s3tkbh9r	109	18	terminally	terminally	RB
cord-311035-s3tkbh9r	109	19	sialylated	sialylate	VBN
cord-311035-s3tkbh9r	109	20	glycans	glycan	NNS
cord-311035-s3tkbh9r	109	21	produced	produce	VBN
cord-311035-s3tkbh9r	109	22	by	by	IN
cord-311035-s3tkbh9r	109	23	human	human	JJ
cord-311035-s3tkbh9r	109	24	cells	cell	NNS
cord-311035-s3tkbh9r	109	25	[	[	-LRB-
cord-311035-s3tkbh9r	109	26	54	54	CD
cord-311035-s3tkbh9r	109	27	]	]	-RRB-
cord-311035-s3tkbh9r	109	28	,	,	,
cord-311035-s3tkbh9r	109	29	which	which	WDT
cord-311035-s3tkbh9r	109	30	can	can	MD
cord-311035-s3tkbh9r	109	31	be	be	VB
cord-311035-s3tkbh9r	109	32	important	important	JJ
cord-311035-s3tkbh9r	109	33	when	when	WRB
cord-311035-s3tkbh9r	109	34	binding	bind	VBG
cord-311035-s3tkbh9r	109	35	interactions	interaction	NNS
cord-311035-s3tkbh9r	109	36	are	be	VBP
cord-311035-s3tkbh9r	109	37	glycan	glycan	NN
cord-311035-s3tkbh9r	109	38	-	-	HYPH
cord-311035-s3tkbh9r	109	39	dependent	dependent	JJ
cord-311035-s3tkbh9r	109	40	as	as	IN
cord-311035-s3tkbh9r	109	41	is	be	VBZ
cord-311035-s3tkbh9r	109	42	seen	see	VBN
cord-311035-s3tkbh9r	109	43	for	for	IN
cord-311035-s3tkbh9r	109	44	some	some	DT
cord-311035-s3tkbh9r	109	45	antibodies	antibody	NNS
cord-311035-s3tkbh9r	109	46	targeting	target	VBG
cord-311035-s3tkbh9r	109	47	viral	viral	JJ
cord-311035-s3tkbh9r	109	48	spikes	spike	NNS
cord-311035-s3tkbh9r	109	49	[	[	-LRB-
cord-311035-s3tkbh9r	109	50	55	55	CD
cord-311035-s3tkbh9r	109	51	]	]	-RRB-
cord-311035-s3tkbh9r	109	52	.	.	.
cord-311035-s3tkbh9r	110	1	However	however	RB
cord-311035-s3tkbh9r	110	2	,	,	,
cord-311035-s3tkbh9r	110	3	this	this	DT
cord-311035-s3tkbh9r	110	4	display	display	NN
cord-311035-s3tkbh9r	110	5	platform	platform	NN
cord-311035-s3tkbh9r	110	6	harnesses	harness	VBZ
cord-311035-s3tkbh9r	110	7	yeast	yeast	NN
cord-311035-s3tkbh9r	110	8	genetics	genetic	NNS
cord-311035-s3tkbh9r	110	9	to	to	TO
cord-311035-s3tkbh9r	110	10	confer	confer	VB
cord-311035-s3tkbh9r	110	11	tremendous	tremendous	JJ
cord-311035-s3tkbh9r	110	12	advantages	advantage	NNS
cord-311035-s3tkbh9r	110	13	for	for	IN
cord-311035-s3tkbh9r	110	14	in	in	FW
cord-311035-s3tkbh9r	110	15	vitro	vitro	FW
cord-311035-s3tkbh9r	110	16	selection	selection	NN
cord-311035-s3tkbh9r	110	17	and	and	CC
cord-311035-s3tkbh9r	110	18	evolution	evolution	NN
cord-311035-s3tkbh9r	110	19	.	.	.
cord-311035-s3tkbh9r	111	1	Using	use	VBG
cord-311035-s3tkbh9r	111	2	yeast	yeast	NN
cord-311035-s3tkbh9r	111	3	display	display	NN
cord-311035-s3tkbh9r	111	4	,	,	,
cord-311035-s3tkbh9r	111	5	large	large	JJ
cord-311035-s3tkbh9r	111	6	diverse	diverse	JJ
cord-311035-s3tkbh9r	111	7	libraries	library	NNS
cord-311035-s3tkbh9r	111	8	can	can	MD
cord-311035-s3tkbh9r	111	9	be	be	VB
cord-311035-s3tkbh9r	111	10	readily	readily	RB
cord-311035-s3tkbh9r	111	11	sorted	sort	VBN
cord-311035-s3tkbh9r	111	12	by	by	IN
cord-311035-s3tkbh9r	111	13	FACS	FACS	NNP
cord-311035-s3tkbh9r	111	14	to	to	TO
cord-311035-s3tkbh9r	111	15	provide	provide	VB
cord-311035-s3tkbh9r	111	16	highquality	highquality	JJ
cord-311035-s3tkbh9r	111	17	data	datum	NNS
cord-311035-s3tkbh9r	111	18	.	.	.
cord-311035-s3tkbh9r	112	1	Separate	separate	JJ
cord-311035-s3tkbh9r	112	2	selections	selection	NNS
cord-311035-s3tkbh9r	112	3	were	be	VBD
cord-311035-s3tkbh9r	112	4	completed	complete	VBN
cord-311035-s3tkbh9r	112	5	at	at	IN
cord-311035-s3tkbh9r	112	6	a	a	DT
cord-311035-s3tkbh9r	112	7	range	range	NN
cord-311035-s3tkbh9r	112	8	of	of	IN
cord-311035-s3tkbh9r	112	9	different	different	JJ
cord-311035-s3tkbh9r	112	10	sACE2	sace2	NN
cord-311035-s3tkbh9r	112	11	concentrations	concentration	NNS
cord-311035-s3tkbh9r	112	12	to	to	TO
cord-311035-s3tkbh9r	112	13	simulate	simulate	VB
cord-311035-s3tkbh9r	112	14	a	a	DT
cord-311035-s3tkbh9r	112	15	titration	titration	NN
cord-311035-s3tkbh9r	112	16	experiment	experiment	NN
cord-311035-s3tkbh9r	112	17	,	,	,
cord-311035-s3tkbh9r	112	18	from	from	IN
cord-311035-s3tkbh9r	112	19	which	which	WDT
cord-311035-s3tkbh9r	112	20	the	the	DT
cord-311035-s3tkbh9r	112	21	data	datum	NNS
cord-311035-s3tkbh9r	112	22	could	could	MD
cord-311035-s3tkbh9r	112	23	be	be	VB
cord-311035-s3tkbh9r	112	24	converted	convert	VBN
cord-311035-s3tkbh9r	112	25	to	to	IN
cord-311035-s3tkbh9r	112	26	quantitative	quantitative	JJ
cord-311035-s3tkbh9r	112	27	changes	change	NNS
cord-311035-s3tkbh9r	112	28	in	in	IN
cord-311035-s3tkbh9r	112	29	apparent	apparent	JJ
cord-311035-s3tkbh9r	112	30	K	k	NN
cord-311035-s3tkbh9r	112	31	D	D	NNP
cord-311035-s3tkbh9r	112	32	on	on	IN
cord-311035-s3tkbh9r	112	33	the	the	DT
cord-311035-s3tkbh9r	112	34	yeast	yeast	NN
cord-311035-s3tkbh9r	112	35	surface	surface	NN
cord-311035-s3tkbh9r	112	36	(	(	-LRB-
cord-311035-s3tkbh9r	112	37	Figure	figure	NN
cord-311035-s3tkbh9r	112	38	2	2	CD
cord-311035-s3tkbh9r	112	39	)	)	-RRB-
cord-311035-s3tkbh9r	112	40	.	.	.
cord-311035-s3tkbh9r	113	1	As	as	IN
cord-311035-s3tkbh9r	113	2	a	a	DT
cord-311035-s3tkbh9r	113	3	surrogate	surrogate	NN
cord-311035-s3tkbh9r	113	4	for	for	IN
cord-311035-s3tkbh9r	113	5	how	how	WRB
cord-311035-s3tkbh9r	113	6	RBD	RBD	NNP
cord-311035-s3tkbh9r	113	7	mutations	mutation	NNS
cord-311035-s3tkbh9r	113	8	may	may	MD
cord-311035-s3tkbh9r	113	9	impact	impact	VB
cord-311035-s3tkbh9r	113	10	expression	expression	NN
cord-311035-s3tkbh9r	113	11	of	of	IN
cord-311035-s3tkbh9r	113	12	the	the	DT
cord-311035-s3tkbh9r	113	13	viral	viral	JJ
cord-311035-s3tkbh9r	113	14	spike	spike	NN
cord-311035-s3tkbh9r	113	15	,	,	,
cord-311035-s3tkbh9r	113	16	the	the	DT
cord-311035-s3tkbh9r	113	17	effects	effect	NNS
cord-311035-s3tkbh9r	113	18	of	of	IN
cord-311035-s3tkbh9r	113	19	mutations	mutation	NNS
cord-311035-s3tkbh9r	113	20	on	on	IN
cord-311035-s3tkbh9r	113	21	RBD	RBD	NNP
cord-311035-s3tkbh9r	113	22	surface	surface	NN
cord-311035-s3tkbh9r	113	23	display	display	NN
cord-311035-s3tkbh9r	113	24	were	be	VBD
cord-311035-s3tkbh9r	113	25	also	also	RB
cord-311035-s3tkbh9r	113	26	assessed	assess	VBN
cord-311035-s3tkbh9r	113	27	in	in	IN
cord-311035-s3tkbh9r	113	28	a	a	DT
cord-311035-s3tkbh9r	113	29	standalone	standalone	JJ
cord-311035-s3tkbh9r	113	30	FACS	FACS	NNP
cord-311035-s3tkbh9r	113	31	selection	selection	NN
cord-311035-s3tkbh9r	113	32	.	.	.
cord-311035-s3tkbh9r	114	1	Quality	quality	NN
cord-311035-s3tkbh9r	114	2	control	control	NN
cord-311035-s3tkbh9r	114	3	pathways	pathway	NNS
cord-311035-s3tkbh9r	114	4	for	for	IN
cord-311035-s3tkbh9r	114	5	protein	protein	NN
cord-311035-s3tkbh9r	114	6	secretion	secretion	NN
cord-311035-s3tkbh9r	114	7	in	in	IN
cord-311035-s3tkbh9r	114	8	yeast	yeast	NN
cord-311035-s3tkbh9r	114	9	can	can	MD
cord-311035-s3tkbh9r	114	10	be	be	VB
cord-311035-s3tkbh9r	114	11	forgiving	forgiving	JJ
cord-311035-s3tkbh9r	114	12	of	of	IN
cord-311035-s3tkbh9r	114	13	misfolded	misfolded	JJ
cord-311035-s3tkbh9r	114	14	protein	protein	NN
cord-311035-s3tkbh9r	114	15	sequences	sequence	NNS
cord-311035-s3tkbh9r	114	16	[	[	-LRB-
cord-311035-s3tkbh9r	114	17	16	16	CD
cord-311035-s3tkbh9r	114	18	]	]	-RRB-
cord-311035-s3tkbh9r	114	19	and	and	CC
cord-311035-s3tkbh9r	114	20	there	there	EX
cord-311035-s3tkbh9r	114	21	are	be	VBP
cord-311035-s3tkbh9r	114	22	residues	residue	NNS
cord-311035-s3tkbh9r	114	23	of	of	IN
cord-311035-s3tkbh9r	114	24	the	the	DT
cord-311035-s3tkbh9r	114	25	RBD	RBD	NNP
cord-311035-s3tkbh9r	114	26	that	that	WDT
cord-311035-s3tkbh9r	114	27	would	would	MD
cord-311035-s3tkbh9r	114	28	ordinarily	ordinarily	RB
cord-311035-s3tkbh9r	114	29	be	be	VB
cord-311035-s3tkbh9r	114	30	buried	bury	VBN
cord-311035-s3tkbh9r	114	31	in	in	IN
cord-311035-s3tkbh9r	114	32	the	the	DT
cord-311035-s3tkbh9r	114	33	context	context	NN
cord-311035-s3tkbh9r	114	34	of	of	IN
cord-311035-s3tkbh9r	114	35	the	the	DT
cord-311035-s3tkbh9r	114	36	full	full	JJ
cord-311035-s3tkbh9r	114	37	S	S	NNP
cord-311035-s3tkbh9r	114	38	protein	protein	NN
cord-311035-s3tkbh9r	114	39	;	;	:
cord-311035-s3tkbh9r	114	40	it	-PRON-	PRP
cord-311035-s3tkbh9r	114	41	therefore	therefore	RB
cord-311035-s3tkbh9r	114	42	remains	remain	VBZ
cord-311035-s3tkbh9r	114	43	to	to	TO
cord-311035-s3tkbh9r	114	44	be	be	VB
cord-311035-s3tkbh9r	114	45	seen	see	VBN
cord-311035-s3tkbh9r	114	46	how	how	WRB
cord-311035-s3tkbh9r	114	47	closely	closely	RB
cord-311035-s3tkbh9r	114	48	the	the	DT
cord-311035-s3tkbh9r	114	49	yeast	yeast	NN
cord-311035-s3tkbh9r	114	50	display	display	NN
cord-311035-s3tkbh9r	114	51	data	datum	NNS
cord-311035-s3tkbh9r	114	52	will	will	MD
cord-311035-s3tkbh9r	114	53	correlate	correlate	VB
cord-311035-s3tkbh9r	114	54	with	with	IN
cord-311035-s3tkbh9r	114	55	equivalent	equivalent	JJ
cord-311035-s3tkbh9r	114	56	experiments	experiment	NNS
cord-311035-s3tkbh9r	114	57	in	in	IN
cord-311035-s3tkbh9r	114	58	more	more	RBR
cord-311035-s3tkbh9r	114	59	physiologically	physiologically	RB
cord-311035-s3tkbh9r	114	60	relevant	relevant	JJ
cord-311035-s3tkbh9r	114	61	expression	expression	NN
cord-311035-s3tkbh9r	114	62	systems	system	NNS
cord-311035-s3tkbh9r	114	63	.	.	.
cord-311035-s3tkbh9r	115	1	Nonetheless	nonetheless	RB
cord-311035-s3tkbh9r	115	2	,	,	,
cord-311035-s3tkbh9r	115	3	the	the	DT
cord-311035-s3tkbh9r	115	4	predicted	predict	VBN
cord-311035-s3tkbh9r	115	5	effects	effect	NNS
cord-311035-s3tkbh9r	115	6	by	by	IN
cord-311035-s3tkbh9r	115	7	yeast	yeast	NN
cord-311035-s3tkbh9r	115	8	display	display	NN
cord-311035-s3tkbh9r	115	9	of	of	IN
cord-311035-s3tkbh9r	115	10	some	some	DT
cord-311035-s3tkbh9r	115	11	mutations	mutation	NNS
cord-311035-s3tkbh9r	115	12	were	be	VBD
cord-311035-s3tkbh9r	115	13	validated	validate	VBN
cord-311035-s3tkbh9r	115	14	using	use	VBG
cord-311035-s3tkbh9r	115	15	full	full	JJ
cord-311035-s3tkbh9r	115	16	length	length	NN
cord-311035-s3tkbh9r	115	17	S	S	NNP
cord-311035-s3tkbh9r	115	18	expressed	express	VBN
cord-311035-s3tkbh9r	115	19	in	in	IN
cord-311035-s3tkbh9r	115	20	human	human	JJ
cord-311035-s3tkbh9r	115	21	cells	cell	NNS
cord-311035-s3tkbh9r	115	22	and	and	CC
cord-311035-s3tkbh9r	115	23	packaged	package	VBN
cord-311035-s3tkbh9r	115	24	in	in	IN
cord-311035-s3tkbh9r	115	25	pseudovirus	pseudovirus	NNP
cord-311035-s3tkbh9r	115	26	[	[	-LRB-
cord-311035-s3tkbh9r	115	27	14	14	CD
cord-311035-s3tkbh9r	115	28	]	]	-RRB-
cord-311035-s3tkbh9r	115	29	.	.	.
cord-311035-s3tkbh9r	116	1	The	the	DT
cord-311035-s3tkbh9r	116	2	library	library	NN
cord-311035-s3tkbh9r	116	3	encoding	encode	VBG
cord-311035-s3tkbh9r	116	4	nearly	nearly	RB
cord-311035-s3tkbh9r	116	5	4,000	4,000	CD
cord-311035-s3tkbh9r	116	6	single	single	JJ
cord-311035-s3tkbh9r	116	7	amino	amino	NN
cord-311035-s3tkbh9r	116	8	acid	acid	NN
cord-311035-s3tkbh9r	116	9	substitutions	substitution	NNS
cord-311035-s3tkbh9r	116	10	in	in	IN
cord-311035-s3tkbh9r	116	11	the	the	DT
cord-311035-s3tkbh9r	116	12	SARS	SARS	NNP
cord-311035-s3tkbh9r	116	13	-	-	HYPH
cord-311035-s3tkbh9r	116	14	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	116	15	RBD	RBD	NNP
cord-311035-s3tkbh9r	116	16	was	be	VBD
cord-311035-s3tkbh9r	116	17	PacBio	pacbio	NN
cord-311035-s3tkbh9r	116	18	sequenced	sequence	VBN
cord-311035-s3tkbh9r	116	19	,	,	,
cord-311035-s3tkbh9r	116	20	providing	provide	VBG
cord-311035-s3tkbh9r	116	21	long	long	JJ
cord-311035-s3tkbh9r	116	22	reads	read	NNS
cord-311035-s3tkbh9r	116	23	that	that	WDT
cord-311035-s3tkbh9r	116	24	match	match	VBP
cord-311035-s3tkbh9r	116	25	untranslated	untranslated	JJ
cord-311035-s3tkbh9r	116	26	nucleotide	nucleotide	NN
cord-311035-s3tkbh9r	116	27	barcodes	barcode	NNS
cord-311035-s3tkbh9r	116	28	to	to	IN
cord-311035-s3tkbh9r	116	29	a	a	DT
cord-311035-s3tkbh9r	116	30	specific	specific	JJ
cord-311035-s3tkbh9r	116	31	protein	protein	NN
cord-311035-s3tkbh9r	116	32	variant	variant	NN
cord-311035-s3tkbh9r	116	33	.	.	.
cord-311035-s3tkbh9r	117	1	Following	follow	VBG
cord-311035-s3tkbh9r	117	2	FACS	FACS	NNP
cord-311035-s3tkbh9r	117	3	-	-	HYPH
cord-311035-s3tkbh9r	117	4	based	base	VBN
cord-311035-s3tkbh9r	117	5	selection	selection	NN
cord-311035-s3tkbh9r	117	6	,	,	,
cord-311035-s3tkbh9r	117	7	only	only	RB
cord-311035-s3tkbh9r	117	8	the	the	DT
cord-311035-s3tkbh9r	117	9	barcodes	barcode	NNS
cord-311035-s3tkbh9r	117	10	are	be	VBP
cord-311035-s3tkbh9r	117	11	read	read	VBN
cord-311035-s3tkbh9r	117	12	to	to	TO
cord-311035-s3tkbh9r	117	13	determine	determine	VB
cord-311035-s3tkbh9r	117	14	how	how	WRB
cord-311035-s3tkbh9r	117	15	favorable	favorable	JJ
cord-311035-s3tkbh9r	117	16	sequence	sequence	NN
cord-311035-s3tkbh9r	117	17	variants	variant	NNS
cord-311035-s3tkbh9r	117	18	are	be	VBP
cord-311035-s3tkbh9r	117	19	enriched	enrich	VBN
cord-311035-s3tkbh9r	117	20	or	or	CC
cord-311035-s3tkbh9r	117	21	deleterious	deleterious	JJ
cord-311035-s3tkbh9r	117	22	sequence	sequence	NN
cord-311035-s3tkbh9r	117	23	variants	variant	NNS
cord-311035-s3tkbh9r	117	24	are	be	VBP
cord-311035-s3tkbh9r	117	25	depleted	deplete	VBN
cord-311035-s3tkbh9r	117	26	.	.	.
cord-311035-s3tkbh9r	118	1	This	this	DT
cord-311035-s3tkbh9r	118	2	resolves	resolve	VBZ
cord-311035-s3tkbh9r	118	3	issues	issue	NNS
cord-311035-s3tkbh9r	118	4	with	with	IN
cord-311035-s3tkbh9r	118	5	Illumina	Illumina	NNP
cord-311035-s3tkbh9r	118	6	sequencing	sequencing	NN
cord-311035-s3tkbh9r	118	7	failing	fail	VBG
cord-311035-s3tkbh9r	118	8	to	to	TO
cord-311035-s3tkbh9r	118	9	cover	cover	VB
cord-311035-s3tkbh9r	118	10	the	the	DT
cord-311035-s3tkbh9r	118	11	full	full	JJ
cord-311035-s3tkbh9r	118	12	cDNA	cdna	NN
cord-311035-s3tkbh9r	118	13	length	length	NN
cord-311035-s3tkbh9r	118	14	,	,	,
cord-311035-s3tkbh9r	118	15	and	and	CC
cord-311035-s3tkbh9r	118	16	because	because	IN
cord-311035-s3tkbh9r	118	17	multiple	multiple	JJ
cord-311035-s3tkbh9r	118	18	barcodes	barcode	NNS
cord-311035-s3tkbh9r	118	19	are	be	VBP
cord-311035-s3tkbh9r	118	20	associated	associate	VBN
cord-311035-s3tkbh9r	118	21	with	with	IN
cord-311035-s3tkbh9r	118	22	any	any	DT
cord-311035-s3tkbh9r	118	23	given	give	VBN
cord-311035-s3tkbh9r	118	24	protein	protein	NN
cord-311035-s3tkbh9r	118	25	variant	variant	NN
cord-311035-s3tkbh9r	118	26	,	,	,
cord-311035-s3tkbh9r	118	27	there	there	EX
cord-311035-s3tkbh9r	118	28	are	be	VBP
cord-311035-s3tkbh9r	118	29	additional	additional	JJ
cord-311035-s3tkbh9r	118	30	internal	internal	JJ
cord-311035-s3tkbh9r	118	31	checks	check	NNS
cord-311035-s3tkbh9r	118	32	for	for	IN
cord-311035-s3tkbh9r	118	33	data	data	NN
cord-311035-s3tkbh9r	118	34	quality	quality	NN
cord-311035-s3tkbh9r	118	35	and	and	CC
cord-311035-s3tkbh9r	118	36	consistency	consistency	NN
cord-311035-s3tkbh9r	118	37	.	.	.
cord-311035-s3tkbh9r	119	1	Despite	despite	IN
cord-311035-s3tkbh9r	119	2	the	the	DT
cord-311035-s3tkbh9r	119	3	limitations	limitation	NNS
cord-311035-s3tkbh9r	119	4	of	of	IN
cord-311035-s3tkbh9r	119	5	a	a	DT
cord-311035-s3tkbh9r	119	6	yeast	yeast	NN
cord-311035-s3tkbh9r	119	7	display	display	NN
cord-311035-s3tkbh9r	119	8	platform	platform	NN
cord-311035-s3tkbh9r	119	9	,	,	,
cord-311035-s3tkbh9r	119	10	the	the	DT
cord-311035-s3tkbh9r	119	11	deep	deep	JJ
cord-311035-s3tkbh9r	119	12	mutational	mutational	JJ
cord-311035-s3tkbh9r	119	13	scan	scan	NN
cord-311035-s3tkbh9r	119	14	of	of	IN
cord-311035-s3tkbh9r	119	15	the	the	DT
cord-311035-s3tkbh9r	119	16	isolated	isolate	VBN
cord-311035-s3tkbh9r	119	17	RBD	RBD	NNP
cord-311035-s3tkbh9r	119	18	provides	provide	VBZ
cord-311035-s3tkbh9r	119	19	a	a	DT
cord-311035-s3tkbh9r	119	20	high	high	JJ
cord-311035-s3tkbh9r	119	21	quality	quality	NN
cord-311035-s3tkbh9r	119	22	and	and	CC
cord-311035-s3tkbh9r	119	23	useful	useful	JJ
cord-311035-s3tkbh9r	119	24	data	datum	NNS
cord-311035-s3tkbh9r	119	25	set	set	VBN
cord-311035-s3tkbh9r	119	26	from	from	IN
cord-311035-s3tkbh9r	119	27	which	which	WDT
cord-311035-s3tkbh9r	119	28	several	several	JJ
cord-311035-s3tkbh9r	119	29	important	important	JJ
cord-311035-s3tkbh9r	119	30	conclusions	conclusion	NNS
cord-311035-s3tkbh9r	119	31	were	be	VBD
cord-311035-s3tkbh9r	119	32	drawn	draw	VBN
cord-311035-s3tkbh9r	119	33	.	.	.
cord-311035-s3tkbh9r	120	1	First	first	RB
cord-311035-s3tkbh9r	120	2	,	,	,
cord-311035-s3tkbh9r	120	3	the	the	DT
cord-311035-s3tkbh9r	120	4	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	120	5	binding	binding	NN
cord-311035-s3tkbh9r	120	6	surface	surface	NN
cord-311035-s3tkbh9r	120	7	of	of	IN
cord-311035-s3tkbh9r	120	8	SARS	SARS	NNP
cord-311035-s3tkbh9r	120	9	-	-	HYPH
cord-311035-s3tkbh9r	120	10	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	120	11	RBD	RBD	NNP
cord-311035-s3tkbh9r	120	12	tolerates	tolerate	VBZ
cord-311035-s3tkbh9r	120	13	surprisingly	surprisingly	RB
cord-311035-s3tkbh9r	120	14	high	high	JJ
cord-311035-s3tkbh9r	120	15	sequence	sequence	NN
cord-311035-s3tkbh9r	120	16	diversity	diversity	NN
cord-311035-s3tkbh9r	120	17	,	,	,
cord-311035-s3tkbh9r	120	18	even	even	RB
cord-311035-s3tkbh9r	120	19	though	though	IN
cord-311035-s3tkbh9r	120	20	it	-PRON-	PRP
cord-311035-s3tkbh9r	120	21	is	be	VBZ
cord-311035-s3tkbh9r	120	22	a	a	DT
cord-311035-s3tkbh9r	120	23	critical	critical	JJ
cord-311035-s3tkbh9r	120	24	site	site	NN
cord-311035-s3tkbh9r	120	25	for	for	IN
cord-311035-s3tkbh9r	120	26	function	function	NN
cord-311035-s3tkbh9r	120	27	[	[	-LRB-
cord-311035-s3tkbh9r	120	28	14	14	CD
cord-311035-s3tkbh9r	120	29	]	]	-RRB-
cord-311035-s3tkbh9r	120	30	.	.	.
cord-311035-s3tkbh9r	121	1	High	high	JJ
cord-311035-s3tkbh9r	121	2	diversity	diversity	NN
cord-311035-s3tkbh9r	121	3	is	be	VBZ
cord-311035-s3tkbh9r	121	4	also	also	RB
cord-311035-s3tkbh9r	121	5	seen	see	VBN
cord-311035-s3tkbh9r	121	6	in	in	IN
cord-311035-s3tkbh9r	121	7	the	the	DT
cord-311035-s3tkbh9r	121	8	ACE2-binding	ace2-binde	VBG
cord-311035-s3tkbh9r	121	9	sites	site	NNS
cord-311035-s3tkbh9r	121	10	of	of	IN
cord-311035-s3tkbh9r	121	11	S	S	NNP
cord-311035-s3tkbh9r	121	12	proteins	protein	NNS
cord-311035-s3tkbh9r	121	13	from	from	IN
cord-311035-s3tkbh9r	121	14	SARS	SARS	NNP
cord-311035-s3tkbh9r	121	15	-	-	HYPH
cord-311035-s3tkbh9r	121	16	related	relate	VBN
cord-311035-s3tkbh9r	121	17	bat	bat	NN
cord-311035-s3tkbh9r	121	18	coronaviruses	coronaviruse	NNS
cord-311035-s3tkbh9r	121	19	,	,	,
cord-311035-s3tkbh9r	121	20	but	but	CC
cord-311035-s3tkbh9r	121	21	this	this	DT
cord-311035-s3tkbh9r	121	22	matches	match	VBZ
cord-311035-s3tkbh9r	121	23	corresponding	correspond	VBG
cord-311035-s3tkbh9r	121	24	diversity	diversity	NN
cord-311035-s3tkbh9r	121	25	in	in	IN
cord-311035-s3tkbh9r	121	26	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	121	27	from	from	IN
cord-311035-s3tkbh9r	121	28	ecologically	ecologically	RB
cord-311035-s3tkbh9r	121	29	diverse	diverse	JJ
cord-311035-s3tkbh9r	121	30	bat	bat	NN
cord-311035-s3tkbh9r	121	31	species	specie	NNS
cord-311035-s3tkbh9r	121	32	[	[	-LRB-
cord-311035-s3tkbh9r	121	33	56	56	CD
cord-311035-s3tkbh9r	121	34	]	]	-RRB-
cord-311035-s3tkbh9r	121	35	and	and	CC
cord-311035-s3tkbh9r	121	36	does	do	VBZ
cord-311035-s3tkbh9r	121	37	not	not	RB
cord-311035-s3tkbh9r	121	38	necessarily	necessarily	RB
cord-311035-s3tkbh9r	121	39	mean	mean	VB
cord-311035-s3tkbh9r	121	40	that	that	IN
cord-311035-s3tkbh9r	121	41	the	the	DT
cord-311035-s3tkbh9r	121	42	RBD	RBD	NNP
cord-311035-s3tkbh9r	121	43	tolerates	tolerate	VBZ
cord-311035-s3tkbh9r	121	44	mutations	mutation	NNS
cord-311035-s3tkbh9r	121	45	for	for	IN
cord-311035-s3tkbh9r	121	46	binding	bind	VBG
cord-311035-s3tkbh9r	121	47	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	121	48	from	from	IN
cord-311035-s3tkbh9r	121	49	a	a	DT
cord-311035-s3tkbh9r	121	50	single	single	JJ
cord-311035-s3tkbh9r	121	51	species	specie	NNS
cord-311035-s3tkbh9r	121	52	.	.	.
cord-311035-s3tkbh9r	122	1	The	the	DT
cord-311035-s3tkbh9r	122	2	deep	deep	JJ
cord-311035-s3tkbh9r	122	3	mutational	mutational	JJ
cord-311035-s3tkbh9r	122	4	scan	scan	NN
cord-311035-s3tkbh9r	122	5	addresses	address	NNS
cord-311035-s3tkbh9r	122	6	this	this	DT
cord-311035-s3tkbh9r	122	7	uncertainty	uncertainty	NN
cord-311035-s3tkbh9r	122	8	and	and	CC
cord-311035-s3tkbh9r	122	9	is	be	VBZ
cord-311035-s3tkbh9r	122	10	further	further	RB
cord-311035-s3tkbh9r	122	11	supported	support	VBN
cord-311035-s3tkbh9r	122	12	by	by	IN
cord-311035-s3tkbh9r	122	13	evidence	evidence	NN
cord-311035-s3tkbh9r	122	14	showing	show	VBG
cord-311035-s3tkbh9r	122	15	that	that	IN
cord-311035-s3tkbh9r	122	16	diverse	diverse	JJ
cord-311035-s3tkbh9r	122	17	RBD	rbd	NN
cord-311035-s3tkbh9r	122	18	sequences	sequence	NNS
cord-311035-s3tkbh9r	122	19	from	from	IN
cord-311035-s3tkbh9r	122	20	bat	bat	NN
cord-311035-s3tkbh9r	122	21	coronaviruses	coronaviruse	NNS
cord-311035-s3tkbh9r	122	22	are	be	VBP
cord-311035-s3tkbh9r	122	23	all	all	DT
cord-311035-s3tkbh9r	122	24	competent	competent	JJ
cord-311035-s3tkbh9r	122	25	for	for	IN
cord-311035-s3tkbh9r	122	26	binding	bind	VBG
cord-311035-s3tkbh9r	122	27	human	human	JJ
cord-311035-s3tkbh9r	122	28	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	122	29	with	with	IN
cord-311035-s3tkbh9r	122	30	varying	vary	VBG
cord-311035-s3tkbh9r	122	31	affinities	affinity	NNS
cord-311035-s3tkbh9r	122	32	[	[	-LRB-
cord-311035-s3tkbh9r	122	33	38	38	CD
cord-311035-s3tkbh9r	122	34	]	]	-RRB-
cord-311035-s3tkbh9r	122	35	.	.	.
cord-311035-s3tkbh9r	123	1	Second	second	RB
cord-311035-s3tkbh9r	123	2	,	,	,
cord-311035-s3tkbh9r	123	3	mutations	mutation	NNS
cord-311035-s3tkbh9r	123	4	were	be	VBD
cord-311035-s3tkbh9r	123	5	found	find	VBN
cord-311035-s3tkbh9r	123	6	in	in	IN
cord-311035-s3tkbh9r	123	7	the	the	DT
cord-311035-s3tkbh9r	123	8	RBD	RBD	NNP
cord-311035-s3tkbh9r	123	9	that	that	WDT
cord-311035-s3tkbh9r	123	10	enhance	enhance	VBP
cord-311035-s3tkbh9r	123	11	binding	binding	NN
cord-311035-s3tkbh9r	123	12	to	to	IN
cord-311035-s3tkbh9r	123	13	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	123	14	,	,	,
cord-311035-s3tkbh9r	123	15	yet	yet	CC
cord-311035-s3tkbh9r	123	16	there	there	EX
cord-311035-s3tkbh9r	123	17	does	do	VBZ
cord-311035-s3tkbh9r	123	18	not	not	RB
cord-311035-s3tkbh9r	123	19	appear	appear	VB
cord-311035-s3tkbh9r	123	20	to	to	TO
cord-311035-s3tkbh9r	123	21	be	be	VB
cord-311035-s3tkbh9r	123	22	positive	positive	JJ
cord-311035-s3tkbh9r	123	23	selective	selective	JJ
cord-311035-s3tkbh9r	123	24	pressure	pressure	NN
cord-311035-s3tkbh9r	123	25	for	for	IN
cord-311035-s3tkbh9r	123	26	these	these	DT
cord-311035-s3tkbh9r	123	27	variants	variant	NNS
cord-311035-s3tkbh9r	123	28	in	in	IN
cord-311035-s3tkbh9r	123	29	the	the	DT
cord-311035-s3tkbh9r	123	30	human	human	JJ
cord-311035-s3tkbh9r	123	31	population	population	NN
cord-311035-s3tkbh9r	123	32	[	[	-LRB-
cord-311035-s3tkbh9r	123	33	14	14	CD
cord-311035-s3tkbh9r	123	34	]	]	-RRB-
cord-311035-s3tkbh9r	123	35	.	.	.
cord-311035-s3tkbh9r	124	1	SARS	SARS	NNP
cord-311035-s3tkbh9r	124	2	-	-	HYPH
cord-311035-s3tkbh9r	124	3	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	124	4	affinity	affinity	NN
cord-311035-s3tkbh9r	124	5	for	for	IN
cord-311035-s3tkbh9r	124	6	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	124	7	is	be	VBZ
cord-311035-s3tkbh9r	124	8	therefore	therefore	RB
cord-311035-s3tkbh9r	124	9	'	'	``
cord-311035-s3tkbh9r	124	10	good	good	JJ
cord-311035-s3tkbh9r	124	11	enough	enough	RB
cord-311035-s3tkbh9r	124	12	,	,	,
cord-311035-s3tkbh9r	124	13	'	'	''
cord-311035-s3tkbh9r	124	14	with	with	IN
cord-311035-s3tkbh9r	124	15	no	no	DT
cord-311035-s3tkbh9r	124	16	additional	additional	JJ
cord-311035-s3tkbh9r	124	17	fitness	fitness	NN
cord-311035-s3tkbh9r	124	18	benefit	benefit	NN
cord-311035-s3tkbh9r	124	19	for	for	IN
cord-311035-s3tkbh9r	124	20	higher	high	JJR
cord-311035-s3tkbh9r	124	21	affinity	affinity	NN
cord-311035-s3tkbh9r	124	22	.	.	.
cord-311035-s3tkbh9r	125	1	It	-PRON-	PRP
cord-311035-s3tkbh9r	125	2	is	be	VBZ
cord-311035-s3tkbh9r	125	3	worth	worth	JJ
cord-311035-s3tkbh9r	125	4	noting	note	VBG
cord-311035-s3tkbh9r	125	5	that	that	IN
cord-311035-s3tkbh9r	125	6	classical	classical	JJ
cord-311035-s3tkbh9r	125	7	SARS	SARS	NNP
cord-311035-s3tkbh9r	125	8	-	-	HYPH
cord-311035-s3tkbh9r	125	9	CoV-1	CoV-1	NNP
cord-311035-s3tkbh9r	125	10	is	be	VBZ
cord-311035-s3tkbh9r	125	11	also	also	RB
cord-311035-s3tkbh9r	125	12	a	a	DT
cord-311035-s3tkbh9r	125	13	highly	highly	RB
cord-311035-s3tkbh9r	125	14	infectious	infectious	JJ
cord-311035-s3tkbh9r	125	15	and	and	CC
cord-311035-s3tkbh9r	125	16	virulent	virulent	JJ
cord-311035-s3tkbh9r	125	17	pathogen	pathogen	NN
cord-311035-s3tkbh9r	125	18	,	,	,
cord-311035-s3tkbh9r	125	19	despite	despite	IN
cord-311035-s3tkbh9r	125	20	having	have	VBG
cord-311035-s3tkbh9r	125	21	weaker	weak	JJR
cord-311035-s3tkbh9r	125	22	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	125	23	affinity	affinity	NN
cord-311035-s3tkbh9r	125	24	[	[	-LRB-
cord-311035-s3tkbh9r	125	25	36	36	CD
cord-311035-s3tkbh9r	125	26	,	,	,
cord-311035-s3tkbh9r	125	27	57	57	CD
cord-311035-s3tkbh9r	125	28	]	]	-RRB-
cord-311035-s3tkbh9r	125	29	.	.	.
cord-311035-s3tkbh9r	126	1	The	the	DT
cord-311035-s3tkbh9r	126	2	rapid	rapid	JJ
cord-311035-s3tkbh9r	126	3	spread	spread	NN
cord-311035-s3tkbh9r	126	4	of	of	IN
cord-311035-s3tkbh9r	126	5	SARS	SARS	NNP
cord-311035-s3tkbh9r	126	6	-	-	HYPH
cord-311035-s3tkbh9r	126	7	CoV	CoV	NNP
cord-311035-s3tkbh9r	126	8	-2	-2	XX
cord-311035-s3tkbh9r	126	9	probably	probably	RB
cord-311035-s3tkbh9r	126	10	has	have	VBZ
cord-311035-s3tkbh9r	126	11	more	more	JJR
cord-311035-s3tkbh9r	126	12	to	to	TO
cord-311035-s3tkbh9r	126	13	do	do	VB
cord-311035-s3tkbh9r	126	14	with	with	IN
cord-311035-s3tkbh9r	126	15	asymptomatic	asymptomatic	JJ
cord-311035-s3tkbh9r	126	16	and	and	CC
cord-311035-s3tkbh9r	126	17	presymptomatic	presymptomatic	JJ
cord-311035-s3tkbh9r	126	18	transmission	transmission	NN
cord-311035-s3tkbh9r	126	19	than	than	IN
cord-311035-s3tkbh9r	126	20	enhanced	enhance	VBN
cord-311035-s3tkbh9r	126	21	receptor	receptor	NN
cord-311035-s3tkbh9r	126	22	binding	binding	NN
cord-311035-s3tkbh9r	126	23	.	.	.
cord-311035-s3tkbh9r	127	1	Third	third	RB
cord-311035-s3tkbh9r	127	2	,	,	,
cord-311035-s3tkbh9r	127	3	mutations	mutation	NNS
cord-311035-s3tkbh9r	127	4	were	be	VBD
cord-311035-s3tkbh9r	127	5	found	find	VBN
cord-311035-s3tkbh9r	127	6	within	within	IN
cord-311035-s3tkbh9r	127	7	the	the	DT
cord-311035-s3tkbh9r	127	8	epitopes	epitope	NNS
cord-311035-s3tkbh9r	127	9	for	for	IN
cord-311035-s3tkbh9r	127	10	monoclonal	monoclonal	JJ
cord-311035-s3tkbh9r	127	11	antibodies	antibody	NNS
cord-311035-s3tkbh9r	127	12	but	but	CC
cord-311035-s3tkbh9r	127	13	maintain	maintain	VBP
cord-311035-s3tkbh9r	127	14	high	high	JJ
cord-311035-s3tkbh9r	127	15	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	127	16	binding	binding	NN
cord-311035-s3tkbh9r	127	17	,	,	,
cord-311035-s3tkbh9r	127	18	and	and	CC
cord-311035-s3tkbh9r	127	19	it	-PRON-	PRP
cord-311035-s3tkbh9r	127	20	is	be	VBZ
cord-311035-s3tkbh9r	127	21	likely	likely	JJ
cord-311035-s3tkbh9r	127	22	that	that	IN
cord-311035-s3tkbh9r	127	23	SARS	SARS	NNP
cord-311035-s3tkbh9r	127	24	-	-	HYPH
cord-311035-s3tkbh9r	127	25	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	127	26	can	can	MD
cord-311035-s3tkbh9r	127	27	easily	easily	RB
cord-311035-s3tkbh9r	127	28	mutate	mutate	VB
cord-311035-s3tkbh9r	127	29	to	to	TO
cord-311035-s3tkbh9r	127	30	escape	escape	VB
cord-311035-s3tkbh9r	127	31	neutralization	neutralization	NN
cord-311035-s3tkbh9r	127	32	without	without	IN
cord-311035-s3tkbh9r	127	33	losing	lose	VBG
cord-311035-s3tkbh9r	127	34	infectivity	infectivity	NN
cord-311035-s3tkbh9r	127	35	[	[	-LRB-
cord-311035-s3tkbh9r	127	36	14	14	CD
cord-311035-s3tkbh9r	127	37	]	]	-RRB-
cord-311035-s3tkbh9r	127	38	.	.	.
cord-311035-s3tkbh9r	128	1	This	this	DT
cord-311035-s3tkbh9r	128	2	agrees	agree	VBZ
cord-311035-s3tkbh9r	128	3	with	with	IN
cord-311035-s3tkbh9r	128	4	selection	selection	NN
cord-311035-s3tkbh9r	128	5	experiments	experiment	NNS
cord-311035-s3tkbh9r	128	6	of	of	IN
cord-311035-s3tkbh9r	128	7	pseudovirus	pseudovirus	NNP
cord-311035-s3tkbh9r	128	8	expressing	express	VBG
cord-311035-s3tkbh9r	128	9	SARS	SARS	NNP
cord-311035-s3tkbh9r	128	10	-	-	HYPH
cord-311035-s3tkbh9r	128	11	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	128	12	S	S	NNP
cord-311035-s3tkbh9r	128	13	variants	variant	NNS
cord-311035-s3tkbh9r	128	14	,	,	,
cord-311035-s3tkbh9r	128	15	in	in	IN
cord-311035-s3tkbh9r	128	16	which	which	WDT
cord-311035-s3tkbh9r	128	17	escape	escape	NN
cord-311035-s3tkbh9r	128	18	mutants	mutant	NNS
cord-311035-s3tkbh9r	128	19	in	in	IN
cord-311035-s3tkbh9r	128	20	the	the	DT
cord-311035-s3tkbh9r	128	21	viral	viral	JJ
cord-311035-s3tkbh9r	128	22	spike	spike	NN
cord-311035-s3tkbh9r	128	23	rapidly	rapidly	RB
cord-311035-s3tkbh9r	128	24	emerge	emerge	VBP
cord-311035-s3tkbh9r	128	25	to	to	IN
cord-311035-s3tkbh9r	128	26	neutralizing	neutralize	VBG
cord-311035-s3tkbh9r	128	27	antibodies	antibody	NNS
cord-311035-s3tkbh9r	128	28	in	in	IN
cord-311035-s3tkbh9r	128	29	a	a	DT
cord-311035-s3tkbh9r	128	30	single	single	JJ
cord-311035-s3tkbh9r	128	31	passage	passage	NN
cord-311035-s3tkbh9r	128	32	[	[	-LRB-
cord-311035-s3tkbh9r	128	33	43	43	CD
cord-311035-s3tkbh9r	128	34	]	]	-RRB-
cord-311035-s3tkbh9r	128	35	.	.	.
cord-311035-s3tkbh9r	129	1	This	this	DT
cord-311035-s3tkbh9r	129	2	has	have	VBZ
cord-311035-s3tkbh9r	129	3	profound	profound	JJ
cord-311035-s3tkbh9r	129	4	implications	implication	NNS
cord-311035-s3tkbh9r	129	5	for	for	IN
cord-311035-s3tkbh9r	129	6	antibody	antibody	NN
cord-311035-s3tkbh9r	129	7	therapy	therapy	NN
cord-311035-s3tkbh9r	129	8	,	,	,
cord-311035-s3tkbh9r	129	9	where	where	WRB
cord-311035-s3tkbh9r	129	10	the	the	DT
cord-311035-s3tkbh9r	129	11	standard	standard	NN
cord-311035-s3tkbh9r	129	12	has	have	VBZ
cord-311035-s3tkbh9r	129	13	become	become	VBN
cord-311035-s3tkbh9r	129	14	combinations	combination	NNS
cord-311035-s3tkbh9r	129	15	of	of	IN
cord-311035-s3tkbh9r	129	16	noncompeting	noncompeting	NN
cord-311035-s3tkbh9r	129	17	monoclonals	monoclonal	NNS
cord-311035-s3tkbh9r	129	18	in	in	IN
cord-311035-s3tkbh9r	129	19	a	a	DT
cord-311035-s3tkbh9r	129	20	cocktail	cocktail	NN
cord-311035-s3tkbh9r	129	21	to	to	TO
cord-311035-s3tkbh9r	129	22	prevent	prevent	VB
cord-311035-s3tkbh9r	129	23	rapid	rapid	JJ
cord-311035-s3tkbh9r	129	24	resistance	resistance	NN
cord-311035-s3tkbh9r	129	25	.	.	.
cord-311035-s3tkbh9r	130	1	It	-PRON-	PRP
cord-311035-s3tkbh9r	130	2	is	be	VBZ
cord-311035-s3tkbh9r	130	3	currently	currently	RB
cord-311035-s3tkbh9r	130	4	unknown	unknown	JJ
cord-311035-s3tkbh9r	130	5	whether	whether	IN
cord-311035-s3tkbh9r	130	6	an	an	DT
cord-311035-s3tkbh9r	130	7	engineered	engineer	VBN
cord-311035-s3tkbh9r	130	8	soluble	soluble	JJ
cord-311035-s3tkbh9r	130	9	decoy	decoy	NN
cord-311035-s3tkbh9r	130	10	receptor	receptor	NN
cord-311035-s3tkbh9r	130	11	,	,	,
cord-311035-s3tkbh9r	130	12	such	such	JJ
cord-311035-s3tkbh9r	130	13	as	as	IN
cord-311035-s3tkbh9r	130	14	sACE2	sACE2	NNS
cord-311035-s3tkbh9r	130	15	2	2	CD
cord-311035-s3tkbh9r	130	16	.v2.4	.v2.4	NFP
cord-311035-s3tkbh9r	130	17	,	,	,
cord-311035-s3tkbh9r	130	18	will	will	MD
cord-311035-s3tkbh9r	130	19	similarly	similarly	RB
cord-311035-s3tkbh9r	130	20	be	be	VB
cord-311035-s3tkbh9r	130	21	susceptible	susceptible	JJ
cord-311035-s3tkbh9r	130	22	to	to	IN
cord-311035-s3tkbh9r	130	23	the	the	DT
cord-311035-s3tkbh9r	130	24	emergence	emergence	NN
cord-311035-s3tkbh9r	130	25	of	of	IN
cord-311035-s3tkbh9r	130	26	viral	viral	JJ
cord-311035-s3tkbh9r	130	27	spike	spike	NN
cord-311035-s3tkbh9r	130	28	variants	variant	NNS
cord-311035-s3tkbh9r	130	29	that	that	WDT
cord-311035-s3tkbh9r	130	30	can	can	MD
cord-311035-s3tkbh9r	130	31	discriminate	discriminate	VB
cord-311035-s3tkbh9r	130	32	between	between	IN
cord-311035-s3tkbh9r	130	33	the	the	DT
cord-311035-s3tkbh9r	130	34	engineered	engineer	VBN
cord-311035-s3tkbh9r	130	35	decoy	decoy	NN
cord-311035-s3tkbh9r	130	36	and	and	CC
cord-311035-s3tkbh9r	130	37	the	the	DT
cord-311035-s3tkbh9r	130	38	native	native	JJ
cord-311035-s3tkbh9r	130	39	receptor	receptor	NN
cord-311035-s3tkbh9r	130	40	.	.	.
cord-311035-s3tkbh9r	131	1	We	-PRON-	PRP
cord-311035-s3tkbh9r	131	2	hypothesize	hypothesize	VBP
cord-311035-s3tkbh9r	131	3	that	that	IN
cord-311035-s3tkbh9r	131	4	engineered	engineer	VBN
cord-311035-s3tkbh9r	131	5	decoys	decoy	NNS
cord-311035-s3tkbh9r	131	6	will	will	MD
cord-311035-s3tkbh9r	131	7	be	be	VB
cord-311035-s3tkbh9r	131	8	broadly	broadly	RB
cord-311035-s3tkbh9r	131	9	active	active	JJ
cord-311035-s3tkbh9r	131	10	against	against	IN
cord-311035-s3tkbh9r	131	11	SARS	SARS	NNP
cord-311035-s3tkbh9r	131	12	-	-	HYPH
cord-311035-s3tkbh9r	131	13	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	131	14	variants	variant	NNS
cord-311035-s3tkbh9r	131	15	and	and	CC
cord-311035-s3tkbh9r	131	16	this	this	DT
cord-311035-s3tkbh9r	131	17	remains	remain	VBZ
cord-311035-s3tkbh9r	131	18	an	an	DT
cord-311035-s3tkbh9r	131	19	active	active	JJ
cord-311035-s3tkbh9r	131	20	area	area	NN
cord-311035-s3tkbh9r	131	21	of	of	IN
cord-311035-s3tkbh9r	131	22	investigation	investigation	NN
cord-311035-s3tkbh9r	131	23	.	.	.
cord-311035-s3tkbh9r	132	1	http://orcid.org/0000-0002-0028-490X	http://orcid.org/0000-0002-0028-490X	NNP
cord-311035-s3tkbh9r	132	2	Papers	Papers	NNPS
cord-311035-s3tkbh9r	132	3	of	of	IN
cord-311035-s3tkbh9r	132	4	special	special	JJ
cord-311035-s3tkbh9r	132	5	note	note	NN
cord-311035-s3tkbh9r	132	6	have	have	VBP
cord-311035-s3tkbh9r	132	7	been	be	VBN
cord-311035-s3tkbh9r	132	8	highlighted	highlight	VBN
cord-311035-s3tkbh9r	132	9	as	as	IN
cord-311035-s3tkbh9r	132	10	either	either	DT
cord-311035-s3tkbh9r	132	11	of	of	IN
cord-311035-s3tkbh9r	132	12	interest	interest	NN
cord-311035-s3tkbh9r	132	13	(	(	-LRB-
cord-311035-s3tkbh9r	132	14	•	•	NNP
cord-311035-s3tkbh9r	132	15	)	)	-RRB-
cord-311035-s3tkbh9r	132	16	or	or	CC
cord-311035-s3tkbh9r	132	17	of	of	IN
cord-311035-s3tkbh9r	132	18	considerable	considerable	JJ
cord-311035-s3tkbh9r	132	19	interest	interest	NN
cord-311035-s3tkbh9r	132	20	Deep	deep	JJ
cord-311035-s3tkbh9r	132	21	mutational	mutational	JJ
cord-311035-s3tkbh9r	132	22	scanning	scanning	NN
cord-311035-s3tkbh9r	132	23	:	:	:
cord-311035-s3tkbh9r	132	24	a	a	DT
cord-311035-s3tkbh9r	132	25	new	new	JJ
cord-311035-s3tkbh9r	132	26	style	style	NN
cord-311035-s3tkbh9r	132	27	of	of	IN
cord-311035-s3tkbh9r	132	28	protein	protein	NN
cord-311035-s3tkbh9r	132	29	science	science	NN
cord-311035-s3tkbh9r	132	30	High	high	JJ
cord-311035-s3tkbh9r	132	31	-	-	HYPH
cord-311035-s3tkbh9r	132	32	resolution	resolution	NN
cord-311035-s3tkbh9r	132	33	mapping	mapping	NN
cord-311035-s3tkbh9r	132	34	of	of	IN
cord-311035-s3tkbh9r	132	35	protein	protein	NN
cord-311035-s3tkbh9r	132	36	sequence	sequence	NN
cord-311035-s3tkbh9r	132	37	-	-	HYPH
cord-311035-s3tkbh9r	132	38	function	function	NN
cord-311035-s3tkbh9r	132	39	relationships	relationship	NNS
cord-311035-s3tkbh9r	133	1	A	a	DT
cord-311035-s3tkbh9r	133	2	fundamental	fundamental	JJ
cord-311035-s3tkbh9r	133	3	protein	protein	NN
cord-311035-s3tkbh9r	133	4	property	property	NN
cord-311035-s3tkbh9r	133	5	,	,	,
cord-311035-s3tkbh9r	133	6	thermodynamic	thermodynamic	JJ
cord-311035-s3tkbh9r	133	7	stability	stability	NN
cord-311035-s3tkbh9r	133	8	,	,	,
cord-311035-s3tkbh9r	133	9	revealed	reveal	VBD
cord-311035-s3tkbh9r	133	10	solely	solely	RB
cord-311035-s3tkbh9r	133	11	from	from	IN
cord-311035-s3tkbh9r	133	12	large	large	JJ
cord-311035-s3tkbh9r	133	13	-	-	HYPH
cord-311035-s3tkbh9r	133	14	scale	scale	NN
cord-311035-s3tkbh9r	133	15	measurements	measurement	NNS
cord-311035-s3tkbh9r	133	16	of	of	IN
cord-311035-s3tkbh9r	133	17	protein	protein	NN
cord-311035-s3tkbh9r	133	18	function	function	NN
cord-311035-s3tkbh9r	134	1	Deep	deep	JJ
cord-311035-s3tkbh9r	134	2	mutational	mutational	JJ
cord-311035-s3tkbh9r	134	3	scanning	scanning	NN
cord-311035-s3tkbh9r	134	4	of	of	IN
cord-311035-s3tkbh9r	134	5	an	an	DT
cord-311035-s3tkbh9r	134	6	antibody	antibody	NN
cord-311035-s3tkbh9r	134	7	against	against	IN
cord-311035-s3tkbh9r	134	8	epidermal	epidermal	JJ
cord-311035-s3tkbh9r	134	9	growth	growth	NN
cord-311035-s3tkbh9r	134	10	factor	factor	NN
cord-311035-s3tkbh9r	134	11	receptor	receptor	NN
cord-311035-s3tkbh9r	134	12	using	use	VBG
cord-311035-s3tkbh9r	134	13	mammalian	mammalian	JJ
cord-311035-s3tkbh9r	134	14	cell	cell	NN
cord-311035-s3tkbh9r	134	15	display	display	NN
cord-311035-s3tkbh9r	134	16	and	and	CC
cord-311035-s3tkbh9r	134	17	massively	massively	RB
cord-311035-s3tkbh9r	134	18	parallel	parallel	JJ
cord-311035-s3tkbh9r	134	19	pyrosequencing	pyrosequencing	NN
cord-311035-s3tkbh9r	134	20	Affinity	affinity	NN
cord-311035-s3tkbh9r	134	21	and	and	CC
cord-311035-s3tkbh9r	134	22	cross	cross	JJ
cord-311035-s3tkbh9r	134	23	-	-	JJ
cord-311035-s3tkbh9r	134	24	reactivity	reactivity	JJ
cord-311035-s3tkbh9r	134	25	engineering	engineering	NN
cord-311035-s3tkbh9r	134	26	of	of	IN
cord-311035-s3tkbh9r	134	27	CTLA4-Ig	CTLA4-Ig	NNP
cord-311035-s3tkbh9r	134	28	to	to	TO
cord-311035-s3tkbh9r	134	29	modulate	modulate	VB
cord-311035-s3tkbh9r	134	30	T	T	NNP
cord-311035-s3tkbh9r	134	31	cell	cell	NN
cord-311035-s3tkbh9r	134	32	costimulation	costimulation	NN
cord-311035-s3tkbh9r	135	1	An	an	DT
cord-311035-s3tkbh9r	135	2	engineered	engineer	VBN
cord-311035-s3tkbh9r	135	3	switch	switch	NN
cord-311035-s3tkbh9r	135	4	in	in	IN
cord-311035-s3tkbh9r	135	5	T	T	NNP
cord-311035-s3tkbh9r	135	6	cell	cell	NN
cord-311035-s3tkbh9r	135	7	receptor	receptor	NN
cord-311035-s3tkbh9r	135	8	specificity	specificity	NN
cord-311035-s3tkbh9r	135	9	leads	lead	VBZ
cord-311035-s3tkbh9r	135	10	to	to	IN
cord-311035-s3tkbh9r	135	11	an	an	DT
cord-311035-s3tkbh9r	135	12	unusual	unusual	JJ
cord-311035-s3tkbh9r	135	13	but	but	CC
cord-311035-s3tkbh9r	135	14	functional	functional	JJ
cord-311035-s3tkbh9r	135	15	binding	binding	NN
cord-311035-s3tkbh9r	135	16	geometry	geometry	NN
cord-311035-s3tkbh9r	135	17	Computational	computational	JJ
cord-311035-s3tkbh9r	135	18	design	design	NN
cord-311035-s3tkbh9r	135	19	of	of	IN
cord-311035-s3tkbh9r	135	20	a	a	DT
cord-311035-s3tkbh9r	135	21	protein	protein	NN
cord-311035-s3tkbh9r	135	22	-	-	HYPH
cord-311035-s3tkbh9r	135	23	based	base	VBN
cord-311035-s3tkbh9r	135	24	enzyme	enzyme	NN
cord-311035-s3tkbh9r	135	25	inhibitor	inhibitor	NN
cord-311035-s3tkbh9r	135	26	Experimental	experimental	JJ
cord-311035-s3tkbh9r	135	27	estimation	estimation	NN
cord-311035-s3tkbh9r	135	28	of	of	IN
cord-311035-s3tkbh9r	135	29	the	the	DT
cord-311035-s3tkbh9r	135	30	effects	effect	NNS
cord-311035-s3tkbh9r	135	31	of	of	IN
cord-311035-s3tkbh9r	135	32	all	all	DT
cord-311035-s3tkbh9r	135	33	amino	amino	NN
cord-311035-s3tkbh9r	135	34	-	-	HYPH
cord-311035-s3tkbh9r	135	35	acid	acid	NN
cord-311035-s3tkbh9r	135	36	mutations	mutation	NNS
cord-311035-s3tkbh9r	135	37	to	to	IN
cord-311035-s3tkbh9r	135	38	HIV	HIV	NNP
cord-311035-s3tkbh9r	135	39	's	's	POS
cord-311035-s3tkbh9r	135	40	envelope	envelope	NN
cord-311035-s3tkbh9r	135	41	protein	protein	NN
cord-311035-s3tkbh9r	135	42	on	on	IN
cord-311035-s3tkbh9r	135	43	viral	viral	JJ
cord-311035-s3tkbh9r	135	44	replication	replication	NN
cord-311035-s3tkbh9r	135	45	in	in	IN
cord-311035-s3tkbh9r	135	46	cell	cell	NN
cord-311035-s3tkbh9r	135	47	culture	culture	NN
cord-311035-s3tkbh9r	136	1	A	a	DT
cord-311035-s3tkbh9r	136	2	platform	platform	NN
cord-311035-s3tkbh9r	136	3	for	for	IN
cord-311035-s3tkbh9r	136	4	functional	functional	JJ
cord-311035-s3tkbh9r	136	5	assessment	assessment	NN
cord-311035-s3tkbh9r	136	6	of	of	IN
cord-311035-s3tkbh9r	136	7	large	large	JJ
cord-311035-s3tkbh9r	136	8	variant	variant	JJ
cord-311035-s3tkbh9r	136	9	libraries	library	NNS
cord-311035-s3tkbh9r	136	10	in	in	IN
cord-311035-s3tkbh9r	136	11	mammalian	mammalian	JJ
cord-311035-s3tkbh9r	136	12	cells	cell	NNS
cord-311035-s3tkbh9r	136	13	Multiplex	multiplex	JJ
cord-311035-s3tkbh9r	136	14	assessment	assessment	NN
cord-311035-s3tkbh9r	136	15	of	of	IN
cord-311035-s3tkbh9r	136	16	protein	protein	NN
cord-311035-s3tkbh9r	136	17	variant	variant	NN
cord-311035-s3tkbh9r	136	18	abundance	abundance	NN
cord-311035-s3tkbh9r	136	19	by	by	IN
cord-311035-s3tkbh9r	136	20	massively	massively	RB
cord-311035-s3tkbh9r	136	21	parallel	parallel	JJ
cord-311035-s3tkbh9r	136	22	sequencing	sequencing	NN
cord-311035-s3tkbh9r	136	23	Mapping	mapping	NN
cord-311035-s3tkbh9r	136	24	interaction	interaction	NN
cord-311035-s3tkbh9r	136	25	sites	site	NNS
cord-311035-s3tkbh9r	136	26	on	on	IN
cord-311035-s3tkbh9r	136	27	human	human	JJ
cord-311035-s3tkbh9r	136	28	chemokine	chemokine	NN
cord-311035-s3tkbh9r	136	29	receptors	receptor	NNS
cord-311035-s3tkbh9r	136	30	by	by	IN
cord-311035-s3tkbh9r	136	31	deep	deep	JJ
cord-311035-s3tkbh9r	136	32	mutational	mutational	JJ
cord-311035-s3tkbh9r	136	33	scanning	scanning	NN
cord-311035-s3tkbh9r	136	34	This	this	DT
cord-311035-s3tkbh9r	136	35	study	study	NN
cord-311035-s3tkbh9r	136	36	established	establish	VBD
cord-311035-s3tkbh9r	136	37	a	a	DT
cord-311035-s3tkbh9r	136	38	simple	simple	JJ
cord-311035-s3tkbh9r	136	39	and	and	CC
cord-311035-s3tkbh9r	136	40	effective	effective	JJ
cord-311035-s3tkbh9r	136	41	method	method	NN
cord-311035-s3tkbh9r	136	42	for	for	IN
cord-311035-s3tkbh9r	136	43	linking	link	VBG
cord-311035-s3tkbh9r	136	44	phenotype	phenotype	NN
cord-311035-s3tkbh9r	136	45	to	to	IN
cord-311035-s3tkbh9r	136	46	a	a	DT
cord-311035-s3tkbh9r	136	47	single	single	JJ
cord-311035-s3tkbh9r	136	48	genotype	genotype	NN
cord-311035-s3tkbh9r	136	49	in	in	IN
cord-311035-s3tkbh9r	136	50	transfected	transfecte	VBN
cord-311035-s3tkbh9r	136	51	human	human	JJ
cord-311035-s3tkbh9r	136	52	cells	cell	NNS
cord-311035-s3tkbh9r	136	53	.	.	.
cord-311035-s3tkbh9r	137	1	This	this	DT
cord-311035-s3tkbh9r	137	2	technical	technical	JJ
cord-311035-s3tkbh9r	137	3	accomplishment	accomplishment	NN
cord-311035-s3tkbh9r	137	4	is	be	VBZ
cord-311035-s3tkbh9r	137	5	necessary	necessary	JJ
cord-311035-s3tkbh9r	137	6	for	for	IN
cord-311035-s3tkbh9r	137	7	selection	selection	NN
cord-311035-s3tkbh9r	137	8	and	and	CC
cord-311035-s3tkbh9r	137	9	deep	deep	JJ
cord-311035-s3tkbh9r	137	10	mutational	mutational	JJ
cord-311035-s3tkbh9r	137	11	scanning	scanning	NN
cord-311035-s3tkbh9r	137	12	in	in	IN
cord-311035-s3tkbh9r	137	13	human	human	JJ
cord-311035-s3tkbh9r	137	14	cells	cell	NNS
cord-311035-s3tkbh9r	138	1	A	a	DT
cord-311035-s3tkbh9r	138	2	comprehensive	comprehensive	JJ
cord-311035-s3tkbh9r	138	3	biophysical	biophysical	JJ
cord-311035-s3tkbh9r	138	4	description	description	NN
cord-311035-s3tkbh9r	138	5	of	of	IN
cord-311035-s3tkbh9r	138	6	pairwise	pairwise	FW
cord-311035-s3tkbh9r	138	7	epistasis	epistasis	NN
cord-311035-s3tkbh9r	138	8	throughout	throughout	IN
cord-311035-s3tkbh9r	138	9	an	an	DT
cord-311035-s3tkbh9r	138	10	entire	entire	JJ
cord-311035-s3tkbh9r	138	11	protein	protein	NN
cord-311035-s3tkbh9r	138	12	domain	domain	NN
cord-311035-s3tkbh9r	138	13	High	high	JJ
cord-311035-s3tkbh9r	138	14	-	-	HYPH
cord-311035-s3tkbh9r	138	15	throughput	throughput	NN
cord-311035-s3tkbh9r	138	16	profiling	profiling	NN
cord-311035-s3tkbh9r	138	17	of	of	IN
cord-311035-s3tkbh9r	138	18	influenza	influenza	NN
cord-311035-s3tkbh9r	138	19	A	a	DT
cord-311035-s3tkbh9r	138	20	virus	virus	NN
cord-311035-s3tkbh9r	138	21	hemagglutinin	hemagglutinin	NN
cord-311035-s3tkbh9r	138	22	gene	gene	NN
cord-311035-s3tkbh9r	138	23	at	at	IN
cord-311035-s3tkbh9r	138	24	single	single	JJ
cord-311035-s3tkbh9r	138	25	-	-	HYPH
cord-311035-s3tkbh9r	138	26	nucleotide	nucleotide	NN
cord-311035-s3tkbh9r	138	27	resolution	resolution	NN
cord-311035-s3tkbh9r	138	28	Deep	deep	JJ
cord-311035-s3tkbh9r	138	29	mutational	mutational	JJ
cord-311035-s3tkbh9r	138	30	scanning	scanning	NN
cord-311035-s3tkbh9r	138	31	of	of	IN
cord-311035-s3tkbh9r	138	32	SARS	SARS	NNP
cord-311035-s3tkbh9r	138	33	-	-	HYPH
cord-311035-s3tkbh9r	138	34	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	138	35	receptor	receptor	NN
cord-311035-s3tkbh9r	138	36	binding	bind	VBG
cord-311035-s3tkbh9r	138	37	domain	domain	NN
cord-311035-s3tkbh9r	138	38	reveals	reveal	VBZ
cord-311035-s3tkbh9r	138	39	constraints	constraint	NNS
cord-311035-s3tkbh9r	138	40	on	on	IN
cord-311035-s3tkbh9r	138	41	folding	fold	VBG
cord-311035-s3tkbh9r	138	42	and	and	CC
cord-311035-s3tkbh9r	138	43	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	138	44	binding	binding	NN
cord-311035-s3tkbh9r	139	1	The	the	DT
cord-311035-s3tkbh9r	139	2	isolated	isolated	JJ
cord-311035-s3tkbh9r	139	3	RBD	RBD	NNP
cord-311035-s3tkbh9r	139	4	of	of	IN
cord-311035-s3tkbh9r	139	5	SARS	SARS	NNP
cord-311035-s3tkbh9r	139	6	-	-	HYPH
cord-311035-s3tkbh9r	139	7	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	139	8	was	be	VBD
cord-311035-s3tkbh9r	139	9	displayed	display	VBN
cord-311035-s3tkbh9r	139	10	on	on	IN
cord-311035-s3tkbh9r	139	11	yeast	yeast	NN
cord-311035-s3tkbh9r	139	12	and	and	CC
cord-311035-s3tkbh9r	139	13	deep	deep	JJ
cord-311035-s3tkbh9r	139	14	mutationally	mutationally	RB
cord-311035-s3tkbh9r	139	15	scanned	scan	VBN
cord-311035-s3tkbh9r	139	16	to	to	TO
cord-311035-s3tkbh9r	139	17	understand	understand	VB
cord-311035-s3tkbh9r	139	18	the	the	DT
cord-311035-s3tkbh9r	139	19	mutational	mutational	JJ
cord-311035-s3tkbh9r	139	20	landscape	landscape	NN
cord-311035-s3tkbh9r	139	21	for	for	IN
cord-311035-s3tkbh9r	139	22	yeast	yeast	NN
cord-311035-s3tkbh9r	139	23	surface	surface	NN
cord-311035-s3tkbh9r	139	24	expression	expression	NN
cord-311035-s3tkbh9r	139	25	(	(	-LRB-
cord-311035-s3tkbh9r	139	26	a	a	DT
cord-311035-s3tkbh9r	139	27	surrogate	surrogate	NN
cord-311035-s3tkbh9r	139	28	for	for	IN
cord-311035-s3tkbh9r	139	29	folding	fold	VBG
cord-311035-s3tkbh9r	139	30	)	)	-RRB-
cord-311035-s3tkbh9r	139	31	and	and	CC
cord-311035-s3tkbh9r	139	32	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	139	33	binding	binding	NN
cord-311035-s3tkbh9r	139	34	.	.	.
cord-311035-s3tkbh9r	140	1	The	the	DT
cord-311035-s3tkbh9r	140	2	data	datum	NNS
cord-311035-s3tkbh9r	140	3	reveal	reveal	VBP
cord-311035-s3tkbh9r	140	4	substantial	substantial	JJ
cord-311035-s3tkbh9r	140	5	sequence	sequence	NN
cord-311035-s3tkbh9r	140	6	diversity	diversity	NN
cord-311035-s3tkbh9r	140	7	is	be	VBZ
cord-311035-s3tkbh9r	140	8	tolerated	tolerate	VBN
cord-311035-s3tkbh9r	140	9	on	on	IN
cord-311035-s3tkbh9r	140	10	the	the	DT
cord-311035-s3tkbh9r	140	11	RBD	RBD	NNP
cord-311035-s3tkbh9r	140	12	surface	surface	NN
cord-311035-s3tkbh9r	140	13	Engineering	Engineering	NNP
cord-311035-s3tkbh9r	140	14	human	human	JJ
cord-311035-s3tkbh9r	140	15	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	140	16	to	to	TO
cord-311035-s3tkbh9r	140	17	optimize	optimize	VB
cord-311035-s3tkbh9r	140	18	binding	bind	VBG
cord-311035-s3tkbh9r	140	19	to	to	IN
cord-311035-s3tkbh9r	140	20	the	the	DT
cord-311035-s3tkbh9r	140	21	spike	spike	NN
cord-311035-s3tkbh9r	140	22	protein	protein	NN
cord-311035-s3tkbh9r	140	23	of	of	IN
cord-311035-s3tkbh9r	140	24	SARS	SARS	NNP
cord-311035-s3tkbh9r	140	25	coronavirus	coronavirus	NN
cord-311035-s3tkbh9r	141	1	2	2	LS
cord-311035-s3tkbh9r	141	2	deep	deep	JJ
cord-311035-s3tkbh9r	141	3	mutationally	mutationally	RB
cord-311035-s3tkbh9r	141	4	scanned	scan	VBN
cord-311035-s3tkbh9r	141	5	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	141	6	expressed	express	VBD
cord-311035-s3tkbh9r	141	7	on	on	IN
cord-311035-s3tkbh9r	141	8	a	a	DT
cord-311035-s3tkbh9r	141	9	human	human	JJ
cord-311035-s3tkbh9r	141	10	cell	cell	NN
cord-311035-s3tkbh9r	141	11	membrane	membrane	NN
cord-311035-s3tkbh9r	141	12	to	to	TO
cord-311035-s3tkbh9r	141	13	identify	identify	VB
cord-311035-s3tkbh9r	141	14	substitutions	substitution	NNS
cord-311035-s3tkbh9r	141	15	that	that	WDT
cord-311035-s3tkbh9r	141	16	enhance	enhance	VBP
cord-311035-s3tkbh9r	141	17	binding	binding	NN
cord-311035-s3tkbh9r	141	18	to	to	IN
cord-311035-s3tkbh9r	141	19	S	S	NNP
cord-311035-s3tkbh9r	141	20	of	of	IN
cord-311035-s3tkbh9r	141	21	SARS	SARS	NNP
cord-311035-s3tkbh9r	141	22	-	-	HYPH
cord-311035-s3tkbh9r	141	23	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	141	24	.	.	.
cord-311035-s3tkbh9r	142	1	This	this	DT
cord-311035-s3tkbh9r	142	2	guided	guide	VBD
cord-311035-s3tkbh9r	142	3	the	the	DT
cord-311035-s3tkbh9r	142	4	engineering	engineering	NN
cord-311035-s3tkbh9r	142	5	of	of	IN
cord-311035-s3tkbh9r	142	6	high	high	JJ
cord-311035-s3tkbh9r	142	7	affinity	affinity	NN
cord-311035-s3tkbh9r	142	8	and	and	CC
cord-311035-s3tkbh9r	142	9	potently	potently	RB
cord-311035-s3tkbh9r	142	10	neutralizing	neutralize	VBG
cord-311035-s3tkbh9r	142	11	decoy	decoy	NN
cord-311035-s3tkbh9r	142	12	receptors	receptor	NNS
cord-311035-s3tkbh9r	142	13	Global	global	JJ
cord-311035-s3tkbh9r	142	14	analysis	analysis	NN
cord-311035-s3tkbh9r	142	15	of	of	IN
cord-311035-s3tkbh9r	142	16	protein	protein	NN
cord-311035-s3tkbh9r	142	17	folding	folding	NN
cord-311035-s3tkbh9r	142	18	using	use	VBG
cord-311035-s3tkbh9r	142	19	massively	massively	RB
cord-311035-s3tkbh9r	142	20	parallel	parallel	JJ
cord-311035-s3tkbh9r	142	21	design	design	NN
cord-311035-s3tkbh9r	142	22	,	,	,
cord-311035-s3tkbh9r	142	23	synthesis	synthesis	NN
cord-311035-s3tkbh9r	142	24	,	,	,
cord-311035-s3tkbh9r	142	25	and	and	CC
cord-311035-s3tkbh9r	142	26	testing	test	VBG
cord-311035-s3tkbh9r	142	27	A	a	DT
cord-311035-s3tkbh9r	142	28	computationally	computationally	RB
cord-311035-s3tkbh9r	142	29	designed	design	VBN
cord-311035-s3tkbh9r	142	30	inhibitor	inhibitor	NN
cord-311035-s3tkbh9r	142	31	of	of	IN
cord-311035-s3tkbh9r	142	32	an	an	DT
cord-311035-s3tkbh9r	142	33	Epstein	Epstein	NNP
cord-311035-s3tkbh9r	142	34	-	-	HYPH
cord-311035-s3tkbh9r	142	35	Barr	Barr	NNP
cord-311035-s3tkbh9r	142	36	viral	viral	NN
cord-311035-s3tkbh9r	143	1	Bcl-2	Bcl-2	NNP
cord-311035-s3tkbh9r	143	2	protein	protein	NN
cord-311035-s3tkbh9r	143	3	induces	induce	VBZ
cord-311035-s3tkbh9r	143	4	apoptosis	apoptosis	NN
cord-311035-s3tkbh9r	143	5	in	in	IN
cord-311035-s3tkbh9r	143	6	infected	infected	JJ
cord-311035-s3tkbh9r	143	7	cells	cell	NNS
cord-311035-s3tkbh9r	144	1	Computationally	computationally	RB
cord-311035-s3tkbh9r	144	2	designed	design	VBN
cord-311035-s3tkbh9r	144	3	high	high	JJ
cord-311035-s3tkbh9r	144	4	specificity	specificity	NN
cord-311035-s3tkbh9r	144	5	inhibitors	inhibitor	NNS
cord-311035-s3tkbh9r	144	6	delineate	delineate	VBP
cord-311035-s3tkbh9r	144	7	the	the	DT
cord-311035-s3tkbh9r	144	8	roles	role	NNS
cord-311035-s3tkbh9r	144	9	of	of	IN
cord-311035-s3tkbh9r	144	10	BCL2	BCL2	NNP
cord-311035-s3tkbh9r	144	11	family	family	NN
cord-311035-s3tkbh9r	144	12	proteins	protein	NNS
cord-311035-s3tkbh9r	144	13	in	in	IN
cord-311035-s3tkbh9r	144	14	cancer	cancer	NN
cord-311035-s3tkbh9r	144	15	Engineered	engineer	VBN
cord-311035-s3tkbh9r	144	16	receptors	receptor	NNS
cord-311035-s3tkbh9r	144	17	for	for	IN
cord-311035-s3tkbh9r	144	18	human	human	JJ
cord-311035-s3tkbh9r	144	19	cytomegalovirus	cytomegalovirus	NN
cord-311035-s3tkbh9r	144	20	that	that	WDT
cord-311035-s3tkbh9r	144	21	are	be	VBP
cord-311035-s3tkbh9r	144	22	orthogonal	orthogonal	JJ
cord-311035-s3tkbh9r	144	23	to	to	IN
cord-311035-s3tkbh9r	144	24	normal	normal	JJ
cord-311035-s3tkbh9r	144	25	human	human	JJ
cord-311035-s3tkbh9r	144	26	biology	biology	NN
cord-311035-s3tkbh9r	144	27	Single	single	JJ
cord-311035-s3tkbh9r	144	28	-	-	HYPH
cord-311035-s3tkbh9r	144	29	mutation	mutation	NN
cord-311035-s3tkbh9r	144	30	fitness	fitness	NN
cord-311035-s3tkbh9r	144	31	landscapes	landscape	NNS
cord-311035-s3tkbh9r	144	32	for	for	IN
cord-311035-s3tkbh9r	144	33	an	an	DT
cord-311035-s3tkbh9r	144	34	enzyme	enzyme	NN
cord-311035-s3tkbh9r	144	35	on	on	IN
cord-311035-s3tkbh9r	144	36	multiple	multiple	JJ
cord-311035-s3tkbh9r	144	37	substrates	substrate	NNS
cord-311035-s3tkbh9r	145	1	reveal	reveal	VB
cord-311035-s3tkbh9r	145	2	specificity	specificity	NN
cord-311035-s3tkbh9r	145	3	is	be	VBZ
cord-311035-s3tkbh9r	145	4	globally	globally	RB
cord-311035-s3tkbh9r	145	5	encoded	encode	VBN
cord-311035-s3tkbh9r	145	6	A	a	DT
cord-311035-s3tkbh9r	145	7	comprehensive	comprehensive	JJ
cord-311035-s3tkbh9r	145	8	,	,	,
cord-311035-s3tkbh9r	145	9	highresolution	highresolution	NNP
cord-311035-s3tkbh9r	145	10	map	map	NN
cord-311035-s3tkbh9r	145	11	of	of	IN
cord-311035-s3tkbh9r	145	12	a	a	DT
cord-311035-s3tkbh9r	145	13	gene	gene	NN
cord-311035-s3tkbh9r	145	14	's	's	POS
cord-311035-s3tkbh9r	145	15	fitness	fitness	NN
cord-311035-s3tkbh9r	145	16	landscape	landscape	NN
cord-311035-s3tkbh9r	145	17	Comprehensive	comprehensive	JJ
cord-311035-s3tkbh9r	145	18	sequence	sequence	NN
cord-311035-s3tkbh9r	145	19	-	-	HYPH
cord-311035-s3tkbh9r	145	20	flux	flux	NN
cord-311035-s3tkbh9r	145	21	mapping	mapping	NN
cord-311035-s3tkbh9r	145	22	of	of	IN
cord-311035-s3tkbh9r	145	23	a	a	DT
cord-311035-s3tkbh9r	145	24	levoglucosan	levoglucosan	NNP
cord-311035-s3tkbh9r	145	25	utilization	utilization	NN
cord-311035-s3tkbh9r	145	26	pathway	pathway	NN
cord-311035-s3tkbh9r	145	27	in	in	IN
cord-311035-s3tkbh9r	145	28	E.	E.	NNP
cord-311035-s3tkbh9r	145	29	coli	coli	VBZ
cord-311035-s3tkbh9r	145	30	Molecular	molecular	JJ
cord-311035-s3tkbh9r	145	31	determinants	determinant	NNS
cord-311035-s3tkbh9r	145	32	of	of	IN
cord-311035-s3tkbh9r	145	33	chaperone	chaperone	NN
cord-311035-s3tkbh9r	145	34	interactions	interaction	NNS
cord-311035-s3tkbh9r	145	35	on	on	IN
cord-311035-s3tkbh9r	145	36	MHC	MHC	NNP
cord-311035-s3tkbh9r	145	37	-	-	HYPH
cord-311035-s3tkbh9r	145	38	I	I	NNP
cord-311035-s3tkbh9r	145	39	for	for	IN
cord-311035-s3tkbh9r	145	40	folding	folding	NN
cord-311035-s3tkbh9r	145	41	and	and	CC
cord-311035-s3tkbh9r	145	42	antigen	antigen	NN
cord-311035-s3tkbh9r	145	43	repertoire	repertoire	NN
cord-311035-s3tkbh9r	146	1	selection	selection	NNP
cord-311035-s3tkbh9r	146	2	Cross	cross	NN
cord-311035-s3tkbh9r	146	3	-	-	NN
cord-311035-s3tkbh9r	146	4	neutralization	neutralization	NN
cord-311035-s3tkbh9r	146	5	of	of	IN
cord-311035-s3tkbh9r	146	6	SARS	SARS	NNP
cord-311035-s3tkbh9r	146	7	-	-	HYPH
cord-311035-s3tkbh9r	146	8	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	146	9	by	by	IN
cord-311035-s3tkbh9r	146	10	a	a	DT
cord-311035-s3tkbh9r	146	11	human	human	JJ
cord-311035-s3tkbh9r	146	12	monoclonal	monoclonal	JJ
cord-311035-s3tkbh9r	146	13	SARS	SARS	NNP
cord-311035-s3tkbh9r	146	14	-	-	HYPH
cord-311035-s3tkbh9r	146	15	CoV	CoV	NNP
cord-311035-s3tkbh9r	146	16	antibody	antibody	NN
cord-311035-s3tkbh9r	146	17	Studies	Studies	NNPS
cord-311035-s3tkbh9r	146	18	in	in	IN
cord-311035-s3tkbh9r	146	19	humanized	humanized	JJ
cord-311035-s3tkbh9r	146	20	mice	mouse	NNS
cord-311035-s3tkbh9r	146	21	and	and	CC
cord-311035-s3tkbh9r	146	22	convalescent	convalescent	JJ
cord-311035-s3tkbh9r	146	23	humans	human	NNS
cord-311035-s3tkbh9r	146	24	yield	yield	VBP
cord-311035-s3tkbh9r	146	25	a	a	DT
cord-311035-s3tkbh9r	146	26	SARS	SARS	NNP
cord-311035-s3tkbh9r	146	27	-	-	HYPH
cord-311035-s3tkbh9r	146	28	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	146	29	antibody	antibody	NN
cord-311035-s3tkbh9r	146	30	cocktail	cocktail	NN
cord-311035-s3tkbh9r	146	31	Potent	potent	JJ
cord-311035-s3tkbh9r	146	32	neutralizing	neutralize	VBG
cord-311035-s3tkbh9r	146	33	antibodies	antibody	NNS
cord-311035-s3tkbh9r	146	34	from	from	IN
cord-311035-s3tkbh9r	146	35	COVID-19	covid-19	JJ
cord-311035-s3tkbh9r	146	36	patients	patient	NNS
cord-311035-s3tkbh9r	146	37	define	define	VBP
cord-311035-s3tkbh9r	146	38	multiple	multiple	JJ
cord-311035-s3tkbh9r	146	39	targets	target	NNS
cord-311035-s3tkbh9r	146	40	of	of	IN
cord-311035-s3tkbh9r	146	41	vulnerability	vulnerability	NN
cord-311035-s3tkbh9r	146	42	Broad	broad	JJ
cord-311035-s3tkbh9r	146	43	neutralization	neutralization	NN
cord-311035-s3tkbh9r	146	44	of	of	IN
cord-311035-s3tkbh9r	146	45	SARS	SARS	NNP
cord-311035-s3tkbh9r	146	46	-	-	HYPH
cord-311035-s3tkbh9r	146	47	related	relate	VBN
cord-311035-s3tkbh9r	146	48	viruses	virus	NNS
cord-311035-s3tkbh9r	146	49	by	by	IN
cord-311035-s3tkbh9r	146	50	human	human	JJ
cord-311035-s3tkbh9r	146	51	monoclonal	monoclonal	JJ
cord-311035-s3tkbh9r	146	52	antibodies	antibody	NNS
cord-311035-s3tkbh9r	147	1	A	a	DT
cord-311035-s3tkbh9r	147	2	human	human	JJ
cord-311035-s3tkbh9r	147	3	monoclonal	monoclonal	JJ
cord-311035-s3tkbh9r	147	4	antibody	antibody	NN
cord-311035-s3tkbh9r	147	5	blocking	block	VBG
cord-311035-s3tkbh9r	147	6	SARS	SARS	NNP
cord-311035-s3tkbh9r	147	7	-	-	HYPH
cord-311035-s3tkbh9r	147	8	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	147	9	infection	infection	NN
cord-311035-s3tkbh9r	148	1	A	a	DT
cord-311035-s3tkbh9r	148	2	noncompeting	noncompeting	JJ
cord-311035-s3tkbh9r	148	3	pair	pair	NN
cord-311035-s3tkbh9r	148	4	of	of	IN
cord-311035-s3tkbh9r	148	5	human	human	JJ
cord-311035-s3tkbh9r	148	6	neutralizing	neutralize	VBG
cord-311035-s3tkbh9r	148	7	antibodies	antibody	NNS
cord-311035-s3tkbh9r	148	8	block	block	VBP
cord-311035-s3tkbh9r	148	9	COVID-19	COVID-19	NNP
cord-311035-s3tkbh9r	148	10	virus	virus	NN
cord-311035-s3tkbh9r	148	11	binding	bind	VBG
cord-311035-s3tkbh9r	148	12	to	to	IN
cord-311035-s3tkbh9r	148	13	its	-PRON-	PRP$
cord-311035-s3tkbh9r	148	14	receptor	receptor	NN
cord-311035-s3tkbh9r	148	15	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	148	16	Isolation	Isolation	NNP
cord-311035-s3tkbh9r	148	17	of	of	IN
cord-311035-s3tkbh9r	148	18	potent	potent	JJ
cord-311035-s3tkbh9r	148	19	SARS	SARS	NNP
cord-311035-s3tkbh9r	148	20	-	-	HYPH
cord-311035-s3tkbh9r	148	21	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	148	22	neutralizing	neutralize	VBG
cord-311035-s3tkbh9r	148	23	antibodies	antibody	NNS
cord-311035-s3tkbh9r	148	24	and	and	CC
cord-311035-s3tkbh9r	148	25	protection	protection	NN
cord-311035-s3tkbh9r	148	26	from	from	IN
cord-311035-s3tkbh9r	148	27	disease	disease	NN
cord-311035-s3tkbh9r	148	28	in	in	IN
cord-311035-s3tkbh9r	148	29	a	a	DT
cord-311035-s3tkbh9r	148	30	small	small	JJ
cord-311035-s3tkbh9r	148	31	animal	animal	NN
cord-311035-s3tkbh9r	148	32	model	model	NN
cord-311035-s3tkbh9r	148	33	Structure	Structure	NNP
cord-311035-s3tkbh9r	148	34	,	,	,
cord-311035-s3tkbh9r	148	35	function	function	NN
cord-311035-s3tkbh9r	148	36	,	,	,
cord-311035-s3tkbh9r	148	37	and	and	CC
cord-311035-s3tkbh9r	148	38	antigenicity	antigenicity	NN
cord-311035-s3tkbh9r	148	39	of	of	IN
cord-311035-s3tkbh9r	148	40	the	the	DT
cord-311035-s3tkbh9r	148	41	SARS	SARS	NNP
cord-311035-s3tkbh9r	148	42	-	-	HYPH
cord-311035-s3tkbh9r	148	43	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	148	44	spike	spike	NN
cord-311035-s3tkbh9r	148	45	glycoprotein	glycoprotein	NN
cord-311035-s3tkbh9r	148	46	Structural	structural	JJ
cord-311035-s3tkbh9r	148	47	insights	insight	NNS
cord-311035-s3tkbh9r	148	48	into	into	IN
cord-311035-s3tkbh9r	148	49	coronavirus	coronavirus	NN
cord-311035-s3tkbh9r	148	50	entry	entry	NN
cord-311035-s3tkbh9r	148	51	SARS	SARS	NNP
cord-311035-s3tkbh9r	148	52	-	-	HYPH
cord-311035-s3tkbh9r	148	53	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	148	54	cell	cell	NN
cord-311035-s3tkbh9r	148	55	entry	entry	NN
cord-311035-s3tkbh9r	148	56	depends	depend	VBZ
cord-311035-s3tkbh9r	148	57	on	on	IN
cord-311035-s3tkbh9r	148	58	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	148	59	and	and	CC
cord-311035-s3tkbh9r	148	60	TMPRSS2	TMPRSS2	NNP
cord-311035-s3tkbh9r	148	61	and	and	CC
cord-311035-s3tkbh9r	148	62	is	be	VBZ
cord-311035-s3tkbh9r	148	63	blocked	block	VBN
cord-311035-s3tkbh9r	148	64	by	by	IN
cord-311035-s3tkbh9r	148	65	a	a	DT
cord-311035-s3tkbh9r	148	66	clinically	clinically	RB
cord-311035-s3tkbh9r	148	67	proven	prove	VBN
cord-311035-s3tkbh9r	148	68	protease	protease	NN
cord-311035-s3tkbh9r	148	69	inhibitor	inhibitor	NN
cord-311035-s3tkbh9r	149	1	A	a	DT
cord-311035-s3tkbh9r	149	2	pneumonia	pneumonia	NN
cord-311035-s3tkbh9r	149	3	outbreak	outbreak	NN
cord-311035-s3tkbh9r	149	4	associated	associate	VBN
cord-311035-s3tkbh9r	149	5	with	with	IN
cord-311035-s3tkbh9r	149	6	a	a	DT
cord-311035-s3tkbh9r	149	7	new	new	JJ
cord-311035-s3tkbh9r	149	8	coronavirus	coronavirus	NN
cord-311035-s3tkbh9r	149	9	of	of	IN
cord-311035-s3tkbh9r	149	10	probable	probable	JJ
cord-311035-s3tkbh9r	149	11	bat	bat	NN
cord-311035-s3tkbh9r	149	12	origin	origin	NN
cord-311035-s3tkbh9r	149	13	Receptor	receptor	NN
cord-311035-s3tkbh9r	149	14	recognition	recognition	NN
cord-311035-s3tkbh9r	149	15	by	by	IN
cord-311035-s3tkbh9r	149	16	the	the	DT
cord-311035-s3tkbh9r	149	17	novel	novel	JJ
cord-311035-s3tkbh9r	149	18	coronavirus	coronavirus	NN
cord-311035-s3tkbh9r	149	19	from	from	IN
cord-311035-s3tkbh9r	149	20	Wuhan	Wuhan	NNP
cord-311035-s3tkbh9r	149	21	:	:	:
cord-311035-s3tkbh9r	149	22	an	an	DT
cord-311035-s3tkbh9r	149	23	analysis	analysis	NN
cord-311035-s3tkbh9r	149	24	based	base	VBN
cord-311035-s3tkbh9r	149	25	on	on	IN
cord-311035-s3tkbh9r	149	26	decade	decade	NN
cord-311035-s3tkbh9r	149	27	-	-	HYPH
cord-311035-s3tkbh9r	149	28	long	long	JJ
cord-311035-s3tkbh9r	149	29	structural	structural	JJ
cord-311035-s3tkbh9r	149	30	studies	study	NNS
cord-311035-s3tkbh9r	149	31	of	of	IN
cord-311035-s3tkbh9r	149	32	SARS	SARS	NNP
cord-311035-s3tkbh9r	149	33	Cryo	Cryo	NNP
cord-311035-s3tkbh9r	149	34	-	-	HYPH
cord-311035-s3tkbh9r	149	35	EM	EM	NNP
cord-311035-s3tkbh9r	149	36	structure	structure	NN
cord-311035-s3tkbh9r	149	37	of	of	IN
cord-311035-s3tkbh9r	149	38	the	the	DT
cord-311035-s3tkbh9r	149	39	2019-nCoV	2019-ncov	CD
cord-311035-s3tkbh9r	149	40	spike	spike	NN
cord-311035-s3tkbh9r	149	41	in	in	IN
cord-311035-s3tkbh9r	149	42	the	the	DT
cord-311035-s3tkbh9r	149	43	prefusion	prefusion	NN
cord-311035-s3tkbh9r	149	44	conformation	conformation	NN
cord-311035-s3tkbh9r	149	45	Angiotensin	Angiotensin	NNP
cord-311035-s3tkbh9r	149	46	-	-	HYPH
cord-311035-s3tkbh9r	149	47	converting	convert	VBG
cord-311035-s3tkbh9r	149	48	enzyme	enzyme	NN
cord-311035-s3tkbh9r	149	49	2	2	CD
cord-311035-s3tkbh9r	149	50	is	be	VBZ
cord-311035-s3tkbh9r	149	51	a	a	DT
cord-311035-s3tkbh9r	149	52	functional	functional	JJ
cord-311035-s3tkbh9r	149	53	receptor	receptor	NN
cord-311035-s3tkbh9r	149	54	for	for	IN
cord-311035-s3tkbh9r	149	55	the	the	DT
cord-311035-s3tkbh9r	149	56	SARS	SARS	NNP
cord-311035-s3tkbh9r	149	57	coronavirus	coronavirus	NN
cord-311035-s3tkbh9r	150	1	This	this	DT
cord-311035-s3tkbh9r	150	2	study	study	NN
cord-311035-s3tkbh9r	150	3	reports	report	VBZ
cord-311035-s3tkbh9r	150	4	the	the	DT
cord-311035-s3tkbh9r	150	5	original	original	JJ
cord-311035-s3tkbh9r	150	6	discovery	discovery	NN
cord-311035-s3tkbh9r	150	7	of	of	IN
cord-311035-s3tkbh9r	150	8	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	150	9	as	as	IN
cord-311035-s3tkbh9r	150	10	the	the	DT
cord-311035-s3tkbh9r	150	11	entry	entry	NN
cord-311035-s3tkbh9r	150	12	receptor	receptor	NN
cord-311035-s3tkbh9r	150	13	for	for	IN
cord-311035-s3tkbh9r	150	14	classical	classical	JJ
cord-311035-s3tkbh9r	150	15	SARS	SARS	NNP
cord-311035-s3tkbh9r	150	16	-	-	HYPH
cord-311035-s3tkbh9r	150	17	CoV-1	CoV-1	NNP
cord-311035-s3tkbh9r	150	18	Functional	functional	JJ
cord-311035-s3tkbh9r	150	19	assessment	assessment	NN
cord-311035-s3tkbh9r	150	20	of	of	IN
cord-311035-s3tkbh9r	150	21	cell	cell	NN
cord-311035-s3tkbh9r	150	22	entry	entry	NN
cord-311035-s3tkbh9r	150	23	and	and	CC
cord-311035-s3tkbh9r	150	24	receptor	receptor	NN
cord-311035-s3tkbh9r	150	25	usage	usage	NN
cord-311035-s3tkbh9r	150	26	for	for	IN
cord-311035-s3tkbh9r	150	27	SARS	SARS	NNP
cord-311035-s3tkbh9r	150	28	-	-	HYPH
cord-311035-s3tkbh9r	150	29	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	150	30	and	and	CC
cord-311035-s3tkbh9r	150	31	other	other	JJ
cord-311035-s3tkbh9r	150	32	lineage	lineage	NN
cord-311035-s3tkbh9r	150	33	B	b	NN
cord-311035-s3tkbh9r	150	34	betacoronaviruses	betacoronaviruses	NNP
cord-311035-s3tkbh9r	151	1	*	*	NFP
cord-311035-s3tkbh9r	152	1	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	152	2	is	be	VBZ
cord-311035-s3tkbh9r	152	3	shown	show	VBN
cord-311035-s3tkbh9r	152	4	to	to	TO
cord-311035-s3tkbh9r	152	5	be	be	VB
cord-311035-s3tkbh9r	152	6	a	a	DT
cord-311035-s3tkbh9r	152	7	shared	share	VBN
cord-311035-s3tkbh9r	152	8	entry	entry	NN
cord-311035-s3tkbh9r	152	9	receptor	receptor	NN
cord-311035-s3tkbh9r	152	10	for	for	IN
cord-311035-s3tkbh9r	152	11	a	a	DT
cord-311035-s3tkbh9r	152	12	clade	clade	NN
cord-311035-s3tkbh9r	152	13	of	of	IN
cord-311035-s3tkbh9r	152	14	SARS	SARS	NNP
cord-311035-s3tkbh9r	152	15	-	-	HYPH
cord-311035-s3tkbh9r	152	16	associated	associate	VBN
cord-311035-s3tkbh9r	152	17	betacoronaviruses	betacoronaviruse	NNS
cord-311035-s3tkbh9r	152	18	,	,	,
cord-311035-s3tkbh9r	152	19	including	include	VBG
cord-311035-s3tkbh9r	152	20	diverse	diverse	JJ
cord-311035-s3tkbh9r	152	21	strains	strain	NNS
cord-311035-s3tkbh9r	152	22	from	from	IN
cord-311035-s3tkbh9r	152	23	bats	bat	NNS
cord-311035-s3tkbh9r	152	24	and	and	CC
cord-311035-s3tkbh9r	152	25	human	human	JJ
cord-311035-s3tkbh9r	152	26	virus	virus	NN
cord-311035-s3tkbh9r	152	27	SARS	SARS	NNP
cord-311035-s3tkbh9r	152	28	-	-	HYPH
cord-311035-s3tkbh9r	152	29	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	152	30	Susceptibility	Susceptibility	NNP
cord-311035-s3tkbh9r	152	31	to	to	IN
cord-311035-s3tkbh9r	152	32	SARS	SARS	NNP
cord-311035-s3tkbh9r	152	33	coronavirus	coronavirus	NN
cord-311035-s3tkbh9r	152	34	S	S	NNP
cord-311035-s3tkbh9r	152	35	protein	protein	NN
cord-311035-s3tkbh9r	152	36	-	-	HYPH
cord-311035-s3tkbh9r	152	37	driven	drive	VBN
cord-311035-s3tkbh9r	152	38	infection	infection	NN
cord-311035-s3tkbh9r	153	1	correlates	correlate	VBZ
cord-311035-s3tkbh9r	153	2	with	with	IN
cord-311035-s3tkbh9r	153	3	expression	expression	NN
cord-311035-s3tkbh9r	153	4	of	of	IN
cord-311035-s3tkbh9r	153	5	angiotensin	angiotensin	NN
cord-311035-s3tkbh9r	153	6	converting	convert	VBG
cord-311035-s3tkbh9r	153	7	enzyme	enzyme	NN
cord-311035-s3tkbh9r	153	8	2	2	CD
cord-311035-s3tkbh9r	153	9	and	and	CC
cord-311035-s3tkbh9r	153	10	infection	infection	NN
cord-311035-s3tkbh9r	153	11	can	can	MD
cord-311035-s3tkbh9r	153	12	be	be	VB
cord-311035-s3tkbh9r	153	13	blocked	block	VBN
cord-311035-s3tkbh9r	153	14	by	by	IN
cord-311035-s3tkbh9r	153	15	soluble	soluble	JJ
cord-311035-s3tkbh9r	153	16	receptor	receptor	NN
cord-311035-s3tkbh9r	153	17	Neutralization	neutralization	NN
cord-311035-s3tkbh9r	153	18	of	of	IN
cord-311035-s3tkbh9r	153	19	SARS	SARS	NNP
cord-311035-s3tkbh9r	153	20	-	-	HYPH
cord-311035-s3tkbh9r	153	21	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	153	22	spike	spike	NN
cord-311035-s3tkbh9r	153	23	pseudotyped	pseudotype	VBD
cord-311035-s3tkbh9r	153	24	virus	virus	NN
cord-311035-s3tkbh9r	153	25	by	by	IN
cord-311035-s3tkbh9r	153	26	recombinant	recombinant	JJ
cord-311035-s3tkbh9r	153	27	ACE2-Ig	ACE2-Ig	NNP
cord-311035-s3tkbh9r	153	28	Inhibition	Inhibition	NNP
cord-311035-s3tkbh9r	153	29	of	of	IN
cord-311035-s3tkbh9r	153	30	SARS	SARS	NNP
cord-311035-s3tkbh9r	153	31	-	-	HYPH
cord-311035-s3tkbh9r	153	32	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	153	33	infections	infection	NNS
cord-311035-s3tkbh9r	153	34	in	in	IN
cord-311035-s3tkbh9r	153	35	engineered	engineer	VBN
cord-311035-s3tkbh9r	153	36	human	human	JJ
cord-311035-s3tkbh9r	153	37	tissues	tissue	NNS
cord-311035-s3tkbh9r	153	38	using	use	VBG
cord-311035-s3tkbh9r	153	39	clinical	clinical	JJ
cord-311035-s3tkbh9r	153	40	-	-	HYPH
cord-311035-s3tkbh9r	153	41	grade	grade	NN
cord-311035-s3tkbh9r	153	42	soluble	soluble	JJ
cord-311035-s3tkbh9r	153	43	human	human	JJ
cord-311035-s3tkbh9r	153	44	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	153	45	Novel	Novel	NNP
cord-311035-s3tkbh9r	153	46	ACE2-IgG1	ACE2-IgG1	NNP
cord-311035-s3tkbh9r	153	47	fusions	fusion	NNS
cord-311035-s3tkbh9r	153	48	with	with	IN
cord-311035-s3tkbh9r	153	49	improved	improve	VBN
cord-311035-s3tkbh9r	153	50	in	in	IN
cord-311035-s3tkbh9r	153	51	vitro	vitro	FW
cord-311035-s3tkbh9r	153	52	and	and	CC
cord-311035-s3tkbh9r	153	53	in	in	IN
cord-311035-s3tkbh9r	153	54	vivo	vivo	NN
cord-311035-s3tkbh9r	153	55	activity	activity	NN
cord-311035-s3tkbh9r	153	56	against	against	IN
cord-311035-s3tkbh9r	153	57	SARS	SARS	NNP
cord-311035-s3tkbh9r	153	58	-	-	HYPH
cord-311035-s3tkbh9r	153	59	CoV2	CoV2	NNP
cord-311035-s3tkbh9r	153	60	Antibody	Antibody	NNP
cord-311035-s3tkbh9r	153	61	cocktail	cocktail	NN
cord-311035-s3tkbh9r	153	62	to	to	IN
cord-311035-s3tkbh9r	153	63	SARS	SARS	NNP
cord-311035-s3tkbh9r	153	64	-	-	HYPH
cord-311035-s3tkbh9r	153	65	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	153	66	spike	spike	NN
cord-311035-s3tkbh9r	153	67	protein	protein	NN
cord-311035-s3tkbh9r	154	1	prevents	prevent	VBZ
cord-311035-s3tkbh9r	154	2	rapid	rapid	JJ
cord-311035-s3tkbh9r	154	3	mutational	mutational	JJ
cord-311035-s3tkbh9r	154	4	escape	escape	NN
cord-311035-s3tkbh9r	154	5	seen	see	VBN
cord-311035-s3tkbh9r	154	6	with	with	IN
cord-311035-s3tkbh9r	154	7	individual	individual	JJ
cord-311035-s3tkbh9r	154	8	antibodies	antibody	NNS
cord-311035-s3tkbh9r	155	1	SARS	SARS	NNP
cord-311035-s3tkbh9r	155	2	-	-	HYPH
cord-311035-s3tkbh9r	155	3	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	155	4	spike	spike	NN
cord-311035-s3tkbh9r	155	5	is	be	VBZ
cord-311035-s3tkbh9r	155	6	able	able	JJ
cord-311035-s3tkbh9r	155	7	to	to	TO
cord-311035-s3tkbh9r	155	8	rapidly	rapidly	RB
cord-311035-s3tkbh9r	155	9	acquire	acquire	VB
cord-311035-s3tkbh9r	155	10	mutations	mutation	NNS
cord-311035-s3tkbh9r	155	11	to	to	TO
cord-311035-s3tkbh9r	155	12	escape	escape	VB
cord-311035-s3tkbh9r	155	13	neutralizing	neutralize	VBG
cord-311035-s3tkbh9r	155	14	monoclonal	monoclonal	JJ
cord-311035-s3tkbh9r	155	15	antibodies	antibody	NNS
cord-311035-s3tkbh9r	155	16	in	in	IN
cord-311035-s3tkbh9r	155	17	tissue	tissue	NN
cord-311035-s3tkbh9r	155	18	culture	culture	NN
cord-311035-s3tkbh9r	155	19	,	,	,
cord-311035-s3tkbh9r	155	20	necessitating	necessitate	VBG
cord-311035-s3tkbh9r	155	21	the	the	DT
cord-311035-s3tkbh9r	155	22	combination	combination	NN
cord-311035-s3tkbh9r	155	23	of	of	IN
cord-311035-s3tkbh9r	155	24	multiple	multiple	JJ
cord-311035-s3tkbh9r	155	25	non	non	JJ
cord-311035-s3tkbh9r	155	26	-	-	JJ
cord-311035-s3tkbh9r	155	27	competing	competing	JJ
cord-311035-s3tkbh9r	155	28	monoclonals	monoclonal	NNS
cord-311035-s3tkbh9r	155	29	in	in	IN
cord-311035-s3tkbh9r	155	30	a	a	DT
cord-311035-s3tkbh9r	155	31	cocktail	cocktail	NN
cord-311035-s3tkbh9r	155	32	to	to	TO
cord-311035-s3tkbh9r	155	33	prevent	prevent	VB
cord-311035-s3tkbh9r	155	34	resistance	resistance	NN
cord-311035-s3tkbh9r	155	35	Structural	structural	JJ
cord-311035-s3tkbh9r	155	36	basis	basis	NN
cord-311035-s3tkbh9r	155	37	for	for	IN
cord-311035-s3tkbh9r	155	38	the	the	DT
cord-311035-s3tkbh9r	155	39	recognition	recognition	NN
cord-311035-s3tkbh9r	155	40	of	of	IN
cord-311035-s3tkbh9r	155	41	the	the	DT
cord-311035-s3tkbh9r	155	42	SARS	SARS	NNP
cord-311035-s3tkbh9r	155	43	-	-	HYPH
cord-311035-s3tkbh9r	155	44	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	155	45	by	by	IN
cord-311035-s3tkbh9r	155	46	full	full	JJ
cord-311035-s3tkbh9r	155	47	-	-	HYPH
cord-311035-s3tkbh9r	155	48	length	length	NN
cord-311035-s3tkbh9r	155	49	human	human	JJ
cord-311035-s3tkbh9r	155	50	ACE2	ACE2	NNP
cord-311035-s3tkbh9r	155	51	Structure	Structure	NNP
cord-311035-s3tkbh9r	155	52	of	of	IN
cord-311035-s3tkbh9r	155	53	SARS	SARS	NNP
cord-311035-s3tkbh9r	155	54	coronavirus	coronavirus	NN
cord-311035-s3tkbh9r	155	55	spike	spike	NN
cord-311035-s3tkbh9r	155	56	receptor	receptor	NN
cord-311035-s3tkbh9r	155	57	-	-	HYPH
cord-311035-s3tkbh9r	155	58	binding	bind	VBG
cord-311035-s3tkbh9r	155	59	domain	domain	NN
cord-311035-s3tkbh9r	155	60	complexed	complexe	VBN
cord-311035-s3tkbh9r	155	61	with	with	IN
cord-311035-s3tkbh9r	155	62	receptor	receptor	NN
cord-311035-s3tkbh9r	155	63	An	an	DT
cord-311035-s3tkbh9r	155	64	improved	improved	JJ
cord-311035-s3tkbh9r	155	65	platform	platform	NN
cord-311035-s3tkbh9r	155	66	for	for	IN
cord-311035-s3tkbh9r	155	67	functional	functional	JJ
cord-311035-s3tkbh9r	155	68	assessment	assessment	NN
cord-311035-s3tkbh9r	155	69	of	of	IN
cord-311035-s3tkbh9r	155	70	large	large	JJ
cord-311035-s3tkbh9r	155	71	protein	protein	NN
cord-311035-s3tkbh9r	155	72	libraries	library	NNS
cord-311035-s3tkbh9r	155	73	in	in	IN
cord-311035-s3tkbh9r	155	74	mammalian	mammalian	JJ
cord-311035-s3tkbh9r	155	75	cells	cell	NNS
cord-311035-s3tkbh9r	155	76	Mammalian	mammalian	JJ
cord-311035-s3tkbh9r	155	77	cell	cell	NN
cord-311035-s3tkbh9r	155	78	surface	surface	NN
cord-311035-s3tkbh9r	155	79	display	display	NN
cord-311035-s3tkbh9r	155	80	for	for	IN
cord-311035-s3tkbh9r	155	81	monoclonal	monoclonal	JJ
cord-311035-s3tkbh9r	155	82	antibody	antibody	NN
cord-311035-s3tkbh9r	155	83	-	-	HYPH
cord-311035-s3tkbh9r	155	84	based	base	VBN
cord-311035-s3tkbh9r	155	85	FACS	FACS	NNP
cord-311035-s3tkbh9r	155	86	selection	selection	NN
cord-311035-s3tkbh9r	155	87	of	of	IN
cord-311035-s3tkbh9r	155	88	viral	viral	JJ
cord-311035-s3tkbh9r	155	89	envelope	envelope	NN
cord-311035-s3tkbh9r	155	90	proteins	protein	NNS
cord-311035-s3tkbh9r	155	91	HIV	HIV	NNP
cord-311035-s3tkbh9r	155	92	vaccine	vaccine	NN
cord-311035-s3tkbh9r	155	93	design	design	NN
cord-311035-s3tkbh9r	155	94	to	to	TO
cord-311035-s3tkbh9r	155	95	target	target	VB
cord-311035-s3tkbh9r	155	96	germline	germline	JJ
cord-311035-s3tkbh9r	155	97	precursors	precursor	NNS
cord-311035-s3tkbh9r	155	98	of	of	IN
cord-311035-s3tkbh9r	155	99	glycan	glycan	NN
cord-311035-s3tkbh9r	155	100	-	-	HYPH
cord-311035-s3tkbh9r	155	101	dependent	dependent	JJ
cord-311035-s3tkbh9r	155	102	broadly	broadly	RB
cord-311035-s3tkbh9r	155	103	neutralizing	neutralize	VBG
cord-311035-s3tkbh9r	155	104	antibodies	antibody	NNS
cord-311035-s3tkbh9r	155	105	Structure	structure	NN
cord-311035-s3tkbh9r	155	106	-	-	HYPH
cord-311035-s3tkbh9r	155	107	based	base	VBN
cord-311035-s3tkbh9r	155	108	design	design	NN
cord-311035-s3tkbh9r	155	109	of	of	IN
cord-311035-s3tkbh9r	155	110	native	native	JJ
cord-311035-s3tkbh9r	155	111	-	-	HYPH
cord-311035-s3tkbh9r	155	112	like	like	JJ
cord-311035-s3tkbh9r	155	113	HIV-1	HIV-1	NNP
cord-311035-s3tkbh9r	155	114	envelope	envelope	NN
cord-311035-s3tkbh9r	155	115	trimers	trimer	NNS
cord-311035-s3tkbh9r	155	116	to	to	TO
cord-311035-s3tkbh9r	155	117	silence	silence	VB
cord-311035-s3tkbh9r	155	118	non	non	JJ
cord-311035-s3tkbh9r	155	119	-	-	JJ
cord-311035-s3tkbh9r	155	120	neutralizing	neutralize	VBG
cord-311035-s3tkbh9r	155	121	epitopes	epitope	NNS
cord-311035-s3tkbh9r	155	122	and	and	CC
cord-311035-s3tkbh9r	155	123	eliminate	eliminate	VB
cord-311035-s3tkbh9r	155	124	CD4	CD4	NNP
cord-311035-s3tkbh9r	155	125	binding	bind	VBG
cord-311035-s3tkbh9r	155	126	Structural	structural	JJ
cord-311035-s3tkbh9r	155	127	architecture	architecture	NN
cord-311035-s3tkbh9r	155	128	of	of	IN
cord-311035-s3tkbh9r	155	129	a	a	DT
cord-311035-s3tkbh9r	155	130	dimeric	dimeric	JJ
cord-311035-s3tkbh9r	155	131	class	class	NN
cord-311035-s3tkbh9r	155	132	C	c	NN
cord-311035-s3tkbh9r	155	133	GPCR	GPCR	NNP
cord-311035-s3tkbh9r	155	134	based	base	VBN
cord-311035-s3tkbh9r	155	135	on	on	IN
cord-311035-s3tkbh9r	155	136	co	co	NN
cord-311035-s3tkbh9r	155	137	-	-	NN
cord-311035-s3tkbh9r	155	138	trafficking	trafficking	NN
cord-311035-s3tkbh9r	155	139	of	of	IN
cord-311035-s3tkbh9r	155	140	sweet	sweet	JJ
cord-311035-s3tkbh9r	155	141	taste	taste	NN
cord-311035-s3tkbh9r	155	142	receptor	receptor	NN
cord-311035-s3tkbh9r	155	143	subunits	subunit	NNS
cord-311035-s3tkbh9r	155	144	Enrich	enrich	VB
cord-311035-s3tkbh9r	155	145	:	:	:
cord-311035-s3tkbh9r	155	146	software	software	NN
cord-311035-s3tkbh9r	155	147	for	for	IN
cord-311035-s3tkbh9r	155	148	analysis	analysis	NN
cord-311035-s3tkbh9r	155	149	of	of	IN
cord-311035-s3tkbh9r	155	150	protein	protein	NN
cord-311035-s3tkbh9r	155	151	function	function	NN
cord-311035-s3tkbh9r	155	152	by	by	IN
cord-311035-s3tkbh9r	155	153	enrichment	enrichment	NN
cord-311035-s3tkbh9r	155	154	and	and	CC
cord-311035-s3tkbh9r	155	155	depletion	depletion	NN
cord-311035-s3tkbh9r	155	156	of	of	IN
cord-311035-s3tkbh9r	155	157	variants	variant	NNS
cord-311035-s3tkbh9r	155	158	HIV-1	HIV-1	NNP
cord-311035-s3tkbh9r	155	159	broadly	broadly	RB
cord-311035-s3tkbh9r	155	160	neutralizing	neutralize	VBG
cord-311035-s3tkbh9r	155	161	antibody	antibody	NN
cord-311035-s3tkbh9r	155	162	precursor	precursor	NN
cord-311035-s3tkbh9r	155	163	B	b	NN
cord-311035-s3tkbh9r	155	164	cells	cell	NNS
cord-311035-s3tkbh9r	155	165	revealed	reveal	VBN
cord-311035-s3tkbh9r	155	166	by	by	IN
cord-311035-s3tkbh9r	155	167	germline	germline	NN
cord-311035-s3tkbh9r	155	168	-	-	HYPH
cord-311035-s3tkbh9r	155	169	targeting	target	VBG
cord-311035-s3tkbh9r	155	170	immunogen	immunogen	NN
cord-311035-s3tkbh9r	155	171	Isolating	Isolating	NNP
cord-311035-s3tkbh9r	155	172	and	and	CC
cord-311035-s3tkbh9r	155	173	engineering	engineer	VBG
cord-311035-s3tkbh9r	155	174	human	human	JJ
cord-311035-s3tkbh9r	155	175	antibodies	antibody	NNS
cord-311035-s3tkbh9r	155	176	using	use	VBG
cord-311035-s3tkbh9r	155	177	yeast	yeast	NN
cord-311035-s3tkbh9r	155	178	surface	surface	NN
cord-311035-s3tkbh9r	155	179	display	display	VB
cord-311035-s3tkbh9r	155	180	The	the	DT
cord-311035-s3tkbh9r	155	181	humanization	humanization	NN
cord-311035-s3tkbh9r	155	182	of	of	IN
cord-311035-s3tkbh9r	155	183	N	n	NN
cord-311035-s3tkbh9r	155	184	-	-	HYPH
cord-311035-s3tkbh9r	155	185	glycosylation	glycosylation	NN
cord-311035-s3tkbh9r	155	186	pathways	pathway	NNS
cord-311035-s3tkbh9r	155	187	in	in	IN
cord-311035-s3tkbh9r	155	188	yeast	yeast	NN
cord-311035-s3tkbh9r	155	189	Glycan	Glycan	NNP
cord-311035-s3tkbh9r	155	190	-	-	HYPH
cord-311035-s3tkbh9r	155	191	dependent	dependent	JJ
cord-311035-s3tkbh9r	155	192	neutralizing	neutralize	VBG
cord-311035-s3tkbh9r	155	193	antibodies	antibody	NNS
cord-311035-s3tkbh9r	155	194	are	be	VBP
cord-311035-s3tkbh9r	155	195	frequently	frequently	RB
cord-311035-s3tkbh9r	155	196	elicited	elicit	VBN
cord-311035-s3tkbh9r	155	197	in	in	IN
cord-311035-s3tkbh9r	155	198	individuals	individual	NNS
cord-311035-s3tkbh9r	155	199	chronically	chronically	RB
cord-311035-s3tkbh9r	155	200	infected	infect	VBN
cord-311035-s3tkbh9r	155	201	with	with	IN
cord-311035-s3tkbh9r	155	202	HIV-1	HIV-1	NNP
cord-311035-s3tkbh9r	155	203	Clade	Clade	NNP
cord-311035-s3tkbh9r	155	204	B	B	NNP
cord-311035-s3tkbh9r	155	205	or	or	CC
cord-311035-s3tkbh9r	155	206	C.	C.	NNP
cord-311035-s3tkbh9r	155	207	AIDS	AIDS	NNP
cord-311035-s3tkbh9r	155	208	Research	Research	NNP
cord-311035-s3tkbh9r	155	209	and	and	CC
cord-311035-s3tkbh9r	155	210	Human	Human	NNP
cord-311035-s3tkbh9r	155	211	Retroviruses	Retroviruses	NNPS
cord-311035-s3tkbh9r	155	212	Exceptional	exceptional	JJ
cord-311035-s3tkbh9r	155	213	diversity	diversity	NN
cord-311035-s3tkbh9r	155	214	and	and	CC
cord-311035-s3tkbh9r	155	215	selection	selection	NN
cord-311035-s3tkbh9r	155	216	pressure	pressure	NN
cord-311035-s3tkbh9r	155	217	on	on	IN
cord-311035-s3tkbh9r	155	218	SARS	SARS	NNP
cord-311035-s3tkbh9r	155	219	-	-	HYPH
cord-311035-s3tkbh9r	155	220	CoV	CoV	NNP
cord-311035-s3tkbh9r	155	221	and	and	CC
cord-311035-s3tkbh9r	155	222	SARS	SARS	NNP
cord-311035-s3tkbh9r	155	223	-	-	HYPH
cord-311035-s3tkbh9r	155	224	CoV-2	CoV-2	NNP
cord-311035-s3tkbh9r	155	225	host	host	NN
cord-311035-s3tkbh9r	155	226	receptor	receptor	NN
cord-311035-s3tkbh9r	155	227	in	in	IN
cord-311035-s3tkbh9r	155	228	bats	bat	NNS
cord-311035-s3tkbh9r	155	229	compared	compare	VBN
cord-311035-s3tkbh9r	155	230	to	to	IN
cord-311035-s3tkbh9r	155	231	other	other	JJ
cord-311035-s3tkbh9r	155	232	mammals	mammal	NNS
cord-311035-s3tkbh9r	155	233	Structural	structural	JJ
cord-311035-s3tkbh9r	155	234	basis	basis	NN
cord-311035-s3tkbh9r	155	235	of	of	IN
cord-311035-s3tkbh9r	155	236	receptor	receptor	NN
cord-311035-s3tkbh9r	155	237	recognition	recognition	NN
cord-311035-s3tkbh9r	155	238	by	by	IN
cord-311035-s3tkbh9r	155	239	SARS	SARS	NNP
cord-311035-s3tkbh9r	155	240	-	-	HYPH
cord-311035-s3tkbh9r	155	241	CoV-2	CoV-2	NNP