id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000125-uvf5qzfd	Kenworthy, Rachael	Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation	2009-09-03		.txt	text/plain	6633	336	54	The interaction between a PAMP and a PRR triggers activation of the interferon (IFN) pathway in mammalian cells, which significantly changes the gene-expression profile in the cells and contributes to the well-documented off-target effect of RNAi. IFN induction is especially problematic in antiviral studies employing RNAi, where the antiviral effect of IFN must be distinguished from that of RNAi. Typical IFN-inducing structure patterns include dsRNA of certain length, single-stranded RNA (ssRNA) containing 5 0 -triphosphates (5 0 -ppp), the dsRNA analogue polyinosinic-polycytidylic acid (poly I:C), and certain dsDNA molecules. Mammalian expression plasmids encoding each of these proteins, as well as the dominant negative (DN) mutants of RIG-I and MDA5, were transfected into 293FT cells with shRNAs and an IFN-b promoter reporter construct.	./cache/cord-000125-uvf5qzfd.txt	./txt/cord-000125-uvf5qzfd.txt