id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002689-qakbp4dz	Brisse, Morgan	Viral inhibitions of PACT-induced RIG-I activation	2017-07-03		.txt	text/plain	1046	56	50	Influenza virus NS1, MERS-CoV 4a, herpesvirus HSV1 Us11, and ebola virus VP35 proteins have all been shown to directly disrupt the interaction between RIG-I and PACT, and hence blocks the ability of PACT to activate RIG-I (4-7). All these viral proteins have RNA binding capabilities, yet it isn't clear from the published reports whether dsRNA is absolutely required to activate RIG-I via PACT induction. When HEK293T cells were transfected with 4a from dsRNA binding MERS-CoV and bCoV-HKU5 and non-dsRNA binding bCoV-HKU4, the IFNβ promoter activity was suppressed in the dsRNA binding 4a expressing cells but was not affected in the non-dsRNA 4a expressing cells, indicating that dsRNA binding is necessary for inhibition of IFN1 production [5] . Like influenza NS1 and MERS-CoV 4a proteins, Us11 protein of HSV1 is a dsRNA binding protein and has been shown to associate with PACT, PKR, MDA5 and RIG-I in addition to 2′,5′-oligoadenylate synthetase (OAS). These known viral proteins have RNA-binding properties, yet it still isn't entirely clear whether RNA binding is an absolute requirement to inhibit PACT-induced RIG-I activation.	./cache/cord-002689-qakbp4dz.txt	./txt/cord-002689-qakbp4dz.txt