id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000981-6vloa2w3	Bálint, Zoltán	Double-Stranded RNA Attenuates the Barrier Function of Human Pulmonary Artery Endothelial Cells	2013-06-03		.txt	text/plain	6086	480	56	The effect of natural and synthetic double-stranded RNA (dsRNA) on hPAECs was investigated using trans-endothelial electric resistance, molecule trafficking, calcium (Ca(2+)) homeostasis, gene expression and proliferation studies. The cells were stimulated with 100 mM histamine or 15 mM 2,5-Di-t-butyl-1,4-benzohydroquinone (BHQ: selective SERCA blocker) in the presence and absence of extracellular Ca 2+ , after incubation for 24 h with 25 mg/mL Poly I:C, 25 mg/mL dsRNA, 2.5 mg/mL L-DNA, 2.5 mg/mL total RNA or control solution. Double-stranded RNA (dsRNA) or its synthetic analogue (Poly I:C) significantly decreased the electric resistance and increased the permeability of the confluent human pulmonary artery endothelial (hPAEC) monolayer as shown on Figure 1 . Altogether, these data suggest that synthetic dsRNA treatment alters the function of SERCA, which inhibits cell proliferation by inducing G1 arrest in the hPAECs and contributing to endothelial dysfunction.	./cache/cord-000981-6vloa2w3.txt	./txt/cord-000981-6vloa2w3.txt