id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002746-qn34eyul	Antzin-Anduetza, Irati	Increasing the CpG dinucleotide abundance in the HIV-1 genomic RNA inhibits viral replication	2017-11-09		.txt	text/plain	9118	491	56	Codon modification of nucleotides (nt) 22-261 or 22-378 in gag inhibited viral replication by decreasing genomic RNA (gRNA) abundance, gRNA stability, Gag expression, virion production and infectivity. To determine if they are necessary for inhibition of HIV-1 replication, codons introducing CpG dinucleotides were mutated back to the wild type codon, which restored efficient Gag expression and infectious virion production. Gag expression and virion production were similar for wild type HIV-1 and HIV-1 ∆22-261 (Fig. 4c) , indicating that RNA or protein sequences in this region are not necessary for these steps of the viral life cycle. When only 11 CpG dinucleotides are inserted into gag in the context of the codon modified sequence (HIV-1 CM22-165), there is a large decrease in infectivity without a substantial loss of gRNA abundance, Gag expression or virion production (Figs. [100] reported that introducing CpG dinucleotides into env inhibited HIV-1 replication by decreasing the abundance of cytoplasmic gRNA, Gag expression, Env expression and infectious virus production.	./cache/cord-002746-qn34eyul.txt	./txt/cord-002746-qn34eyul.txt