id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003382-v3w1wi5c	Rahmatpanah, Farah	Airway epithelial cells prime plasmacytoid dendritic cells to respond to pathogens via secretion of growth factors	2018-10-02		.txt	text/plain	5543	342	56	We examined the effect of human primary bronchial epithelial cells (PBECs) on PDC functions by performing RNA-sequencing of PDCs after co-culture with air liquid interface differentiated PBECs. Functional analysis revealed that PDCs co-cultured with PBECs displayed upregulation of type I IFN production and response genes. In keeping with the RNA-seq data, we observe increased secretion of type I IFN and other cytokines in response to influenza in PDCs co-cultured with PBECs. The PDCs also primed Th1 responses in T cells. PDCs exposed to PBECs secreted significantly higher levels of type I IFNs (p = 0.0001) on stimulation with influenza as compared to PDCs cultured alone (Fig. 3a) , which is in keeping with the RNA-seq data. In keeping with the activation genes in RNA-seq, we also observed increased secretion of type I IFN from influenza stimulated PDCs co-cultured with PBECs. The expression of TLR9 and ZBP1 was upregulated.	./cache/cord-003382-v3w1wi5c.txt	./txt/cord-003382-v3w1wi5c.txt