id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-005654-n9u2em10	Campbell, David A.	Apparent discontinuous transcription of Trypanosoma brucei variant surface antigen genes	1984		.txt	text/plain	4241	218	58	41 The repeated mini-exon sequence that encodes the first 35 base pairs of all variant surface antigen mRNAs of Trypanosoma brucei directs the synthesis of a discrete I37-nucleotide transcript. 41 The repeated mini-exon sequence that encodes the first 35 base pairs of all variant surface antigen mRNAs of Trypanosoma brucei directs the synthesis of a discrete I37-nucleotide transcript. This result could be interpreted as protection by a short RNA of 137 nucleotides derived from the mini-exon repeats and/or by a longer transcript with a discontinuity at this position in the RNA/DNA duplex. The second method to detect and size transcripts from the mini-exon repeat used Northern blot analysis 31 • For this procedure, total trypanosome RNA was resolved by polyacrylamide gel electrophoresis (PAGE) in denaturing conditions, transferred to GeneScreen membrane 32 and hybridized with a [5/ -32 P]_labelled, synthetic oligonucleotide complementary to positions + 117 to +133.	./cache/cord-005654-n9u2em10.txt	./txt/cord-005654-n9u2em10.txt