id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-006826-vnmg0zid	Rayaprolu, Vamseedhar	Length of encapsidated cargo impacts stability and structure of in vitro assembled alphavirus core-like particles	2017-12-06		.txt	text/plain	6543	338	56	In this work, we wanted to determine how the length of the cargo impacts the stability and structure of the assembled CLPs. We hypothesized that cargo neutralizes the basic region of the alphavirus capsid protein and if the cargo is long enough, it will also act to scaffold the CP monomers together. Modest differences in particle size on DLS and EM suggest that changes in light scattering intensity indicate that similar amounts of CLPs were formed using 48hp, 48lin, and 90mer oligos than with the shorter 27mer ( figure 1(B) ). From our ionic strength stability results (figure 1(B)), we postulated that longer cargo that functioned to both neutralize charge and scaffold CP would favor the formation of complete, closed, spherical cores. This model provides an explanation for the structural defects observed in the cryo-EM classification, but does not account for the possible role of longer cargo in scaffolding or bridging between CP monomers to promote CLP assembly.	./cache/cord-006826-vnmg0zid.txt	./txt/cord-006826-vnmg0zid.txt