id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007181-qpahuqld	YOGO, Yoshiaki	Polyadenylate in the Virion RNA of Mouse Hepatitis Virus	1977-10-17		.txt	text/plain	2058	122	59	Sedimentation Analysis-PH]Virion RNA dissolved in 0.1 ml NET was layered on a 15-30% sucrose gradient in NET containing 0.3% SDS, then sedimented at 48,000 rpm for 90 min at room temperature in an SW 50.1 rotor. Isolation of Poly(A)-fHJAdenosine-or P'P]labeled MH virion RNA containing 50 (ig of carrier tRNA was digested with a combination of ribonuclease A (2//g) and Tl (50 units The hydrolysate was neutralized with HC1O, at 0°C and the precipitate was removed by centrifugation. We therefore extracted RNA from [*H]uridine-labeled MH virions with phenol/chloroform as well as 1 % SDS, and RNA's obtained by both methods were compared by sucrose gradient centrifugation. To isolate poly(A), ['HJadenosine-labeled total virion RNA was digested with a combination of ribonuclease A and Tl. The digest was adjusted to 0.3 M NaCl-0.001 M EDTA-0.02 M Tris-HCl (pH 7.5)-7 M urea and applied to a DEAE-Sephadex column equilibrated with the same buffer.	./cache/cord-007181-qpahuqld.txt	./txt/cord-007181-qpahuqld.txt