id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007463-8g0zklzy	Pocock, D.H.	Characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis	2002-11-13		.txt	text/plain	1932	91	49	The 3′ terminal labelling method for the analysis of genome profiles used in this study required only 1 ng of viral RNA, an increase of 1000-fold in sensitivity over ethidium bromide staining for detecting all rotavirus genome segments. To investigate the latter, mixtures containing known amounts of ds-RNA from two rotavirus isolates with disparate genome profiles (UK strain and a C7 type) were used to simulate the type of samples that might be recovered from calves concurrently infected with these two viruses. After 3' terminal labelling the RNA segments were analysed by electrophoresis (Fig. 3) profiles, and meant that in a faecal sample containing two rotaviruses if one virus was present at a concentration 10-fold less than the other, i.e. 1 log10 dilution, it would not be detected. Rotaviruses isolated from 43 sub-clinically infected calves were characterised by genome profile analysis.	./cache/cord-007463-8g0zklzy.txt	./txt/cord-007463-8g0zklzy.txt