id author title date pages extension mime words sentences flesch summary cache txt cord-008541-0u2fatbg Bujarski, Jozef J. Molecular Studies of Genetic RNA–RNA Recombination in Brome Mosaic Virus 2008-02-28 .txt text/plain 6347 356 49 The idea was to develop an infectious RNA3 molecule stable in infection with a possibility of inserting sequences of interest and studying their recombinational activity. This indicated that progeny recombinants easily accumulated, because they easily outcompeted their parental RNAs. Figure 3 shows that all legitimate crossover events occurred within the long (197-to 220-nt) 3' region of M4 or DM4 (homologous among three BMV RNA components) and the corresponding part of either wt RNAl or wt RNA2. The recombination activity between pairs of these mutants was determined by coinoculation rearrangements was tested by inserting long palindromic sequences into RNA3 molecules (Section IV,D). The sequence of the inserted region and the presence of a marker mutation a t the 3' end indicated that B x 4 might have been formed through rearrangements between two mutant B RNA3 molecules. ./cache/cord-008541-0u2fatbg.txt ./txt/cord-008541-0u2fatbg.txt