id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-009662-ntjngiem	Moulton, Jon D.	Using Morpholinos to Control Gene Expression	2007-01-15		.txt	text/plain	11606	583	48	When delivering Morpholinos to cell cultures using Endo-Porter, starting with a 10 µM Morpholino concentration for both fluorescent delivery assays and functional experiments increases the chances that the fluorescence will be visible in the cytosol and that the first functional experiment will produce measurable results. However, assaying only for a phenotypic effect becomes problematic if the expected change in phenotype does not occur; if antisense activity is not separately assessed at the level of protein concentration or mRNA mass, the experimenter will not be able to discern whether (1) the oligo failed to reach and interact with its target mRNA to produce the knockdown or splice-block, or (2) the knockdown or spliceblock was successful but did not cause the expected phenotypic change. The negative control shows that the effects observed during the antisense experiment are due to the sequence of the targeting oligo and not to the backbone chemistry of the Morpholino or the cytosolic delivery method used.	./cache/cord-009662-ntjngiem.txt	./txt/cord-009662-ntjngiem.txt