id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-009943-fzynh14x	Ai‐Nakib, W.	Detection of Human Rhinoviruses and Their Molecular Relationship Using cDNA Probes	2005-12-11		.txt	text/plain	2879	158	58	Further, evidence is provided in this study that indicates that it would be feasible to use cDNA probes to detect human rhinoviruses in nasal washings. In a preliminary study we found that this probe does indeed cross-hybridize with a number of human rhinoviruses indicating the feasibility of cDNA:RNA hybridization for rhinovirus detection [Al-Nakib et al, 19861 . We have now extended these studies to include a larger series of human rhinoviruses (totalling some 56 viruses) and looked in more detail at the molecular relationship between these viruses, the limits of detection, and the feasibility of applying these procedures for the direct detection of viral RNA in nasal washings. However, in addition to detecting virus at lower titres, the strength of the hybridization signals obtained with 20 x SSC-37 % formaldehyde were particularly strong at all virus dilutions in nasal washings compared with phenol/chloroform extraction, indicating that more viral RNA has been immobilised onto the nitrocellulose filters.	./cache/cord-009943-fzynh14x.txt	./txt/cord-009943-fzynh14x.txt