id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-010045-eqzs01au	Britton, P.	Sequence of the nucleoprotein gene from a virulent British field isolate of transmissible gastroenteritis virus and its expression in Saccharomyces cerevisiae	2006-10-27		.txt	text/plain	6185	353	57	Yeast cells containing recombinant plasmids, with the nucleoprotein gene in the correct orientation, produced a polypeptide of M, 47000, identical to the viral product, that reacted with a specific monoclonal antibody. Three recombinant plasmids, shown to contain cDNA inserts of 1.5 kbp and designated pTS13-2, pTS15-1 and pTSi 5-2, were labelled with p^S]-dATP by nick translation and hybridized to glyoxylated TGEV mRNA species separated on 1 % agarose gels as described in Experimentat procedures. The plasmid also contained the smaller 3' ORF which initiates, in a different reading frame, 6 bp 3' from the end of the nucleoprotein gene and terminates 166 bp 5' to the Drall Sma\ combined site between the TGEV cDNA insert and the pUC9 DNA. A recombinant plasmid, pYNGI, was found to contain the TGEV nucleoprotein gene in the correct orientation for expression under the control of the yeast GAL1 promoter.	./cache/cord-010045-eqzs01au.txt	./txt/cord-010045-eqzs01au.txt