id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-011803-9122f1zc	Zhao, Yang	The RNA quality control pathway nonsense-mediated mRNA decay targets cellular and viral RNAs to restrict KSHV	2020-07-03		.txt	text/plain	8355	493	50	Collectively, our study describes an intricate relationship between cellular targets of an RNA quality control pathway and KSHV lytic gene expression, and demonstrates that NMD can function as a cell intrinsic restriction mechanism acting upon DNA viruses. EJCs are deposited at the exon-exon junction, thus we determined whether the EJC component eukaryotic translation initiation factor 4A3 (eIF4A3) is associated with spliced KSHV RNAs. To test EJC association with KSHV transcripts we performed eIF4A3 formaldehyde crosslinking RNA immunoprecipitation (fRIP) coupled to reverse transcription quantitative PCR (RT-qPCR) on lytic iSLK.219 and TREx-BCBL-RTA cells (Fig. 1g, h) . Leveraging p-UPF1 fRIP-seq we identified NMD targets transcriptome-wide in both latent and lytic PEL cells and targets include both host and viral RNAs. Remarkably, the mRNA encoding RTA, the master transcription factor governing KSHV reactivation, is targeted by NMD via its 3′UTR and silencing of UPF1 is sufficient to potentiate RTA-mediated transactivation.	./cache/cord-011803-9122f1zc.txt	./txt/cord-011803-9122f1zc.txt