id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-027865-p1epjn51	Sterchi, Diane L.	Molecular pathology	2020-06-22		.txt	text/plain	13228	841	54	ISH is a method of localizing and detecting specific mRNA sequences in preserved tissue sections or cell preparations by hybridizing the complementary strand of a nucleotide probe to the sequence of interest. (See Data Sheet Kappa and Lambda Probe ISH Kit, Novacastra.) (Fig. 21.3.) Chromogenic in situ hybridization (CISH) is a method 'that enables the detection of gene expression in the nucleus using a conventional histochemical reaction' (White 2005) ; it is used for the detection of abnormal genes and to identify a gene therapy treatment direction. However, FISH still has an advantage over chromogenic methods for labeling specific nucleic acid sequences in cells and tissues. This may be done by using the normal detection procedure for the ISH method on dots of labeled nucleic acid sequence and a labeled control applied at matching descending concentrations on a positively charged nylon membrane.	./cache/cord-027865-p1epjn51.txt	./txt/cord-027865-p1epjn51.txt