id author title date pages extension mime words sentences flesch summary cache txt cord-027975-77544sed Tars, Kaspars ssRNA Phages: Life Cycle, Structure and Applications 2020-06-30 .txt text/plain 11655 582 55 Several of the studied ssRNA characteristics, such as coat protein–RNA interactions and the ability to readily form virus-like particles in recombinant expression systems, have fueled many practical applications such as RNA labeling and tracking systems and vaccine development. Bacteriophages belonging to the family Leviviridae are among the simplest known viruses, exhibiting positive-sense single-stranded RNA (ssRNA) genomes of just 3.5-4.5 kilobases, typically encoding only 4 proteins. Due to their simplicity, ssRNA phages have been used as models to study various processes in molecular biology and virology, including translation repression, RNA-protein interactions and virus evolution. For quite some time, the only available structural information about protein-RNA interactions in ssRNA phage particles came from the studies of CP dimers in complex with a 19 nucleotide-long stem-loop fragment known as TR (translation repression) from the genome region located around the replicase start codon in bacteriophage MS2 ). ./cache/cord-027975-77544sed.txt ./txt/cord-027975-77544sed.txt