id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-048198-zjufx4fo	Pasternak, Alexander O.	Sequence requirements for RNA strand transfer during nidovirus discontinuous subgenomic RNA synthesis	2001-12-17		.txt	text/plain	7614	366	58	Here we present results of a comprehensive co-variation mutagenesis study of equine arteritis virus TRSs, demonstrating that discontinuous RNA synthesis depends not only on base pairing between sense leader TRS and antisense body TRS, but also on the primary sequence of the body TRS. Synthesis of sg mRNAs initially was proposed to be primed by free leader transcripts, which would base-pair to the complementary TRS regions in the full-length minus strand, and would be extended subsequently to make sg plus strands ( Figure 1B ; Baric et al., 1983 Baric et al., , 1985 . 7220±7228, 2001 Using site-directed mutagenesis of TRSs of the arterivirus equine arteritis virus (EAV), we have shown previously that base pairing between the sense leader TRS and antisense body TRSs is crucial for sg mRNA synthesis (van Marle et al., 1999a) .	./cache/cord-048198-zjufx4fo.txt	./txt/cord-048198-zjufx4fo.txt