id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-048204-6lvn10f4	Shi, Stephanie T.	Heterogeneous nuclear ribonucleoprotein A1 regulates RNA synthesis of a cytoplasmic virus	2000-09-01		.txt	text/plain	7656	378	56	In order to demonstrate the involvement of hnRNP A1 in MHV RNA replication and transcription, we established several DBT cell lines stably expressing either the wildtype (wt) hnRNP A1 or a C-terminus-truncated mutant lacking the M9 sequence and part of the glycine-rich domain. We showed that the mutant hnRNP A1, which was localized predominantly in the cytoplasm, exhibited dominant-negative effects on viral genomic RNA replication and subgenomic mRNA transcription. Cells infected with P0 viruses did not yield detectable amounts of DIssE, but contained the naturally occurring A59 DI RNA, whose replication was inhibited more strongly than the synthesis of MHV genomic and subgenomic RNAs in DBT-A1DC cells ( Figures 5B, lanes 8±10 and 6B, lanes 1± 3). In the present study, we established that MHV RNA transcription and replication were enhanced by overexpression of the wt hnRNP A1 protein, but inhibited by expression of a dominant-negative hnRNP A1 mutant in DBT cell lines.	./cache/cord-048204-6lvn10f4.txt	./txt/cord-048204-6lvn10f4.txt