id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-048222-1pq6dkl5	Imbeaud, Sandrine	Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces	2005-03-30		.txt	text/plain	7001	313	47	With that prospect in mind, and with the aim of anticipating future standards by pre-normative research, we identified and tested two software packages recently developed to gauge the integrity of RNA samples with a user-independent strategy: one open source, the degradometer software for calculation of the degradation factor and 'true' 28S:18S ratio based on peak heights (24) and the freely available RIN algorithm of the Agilent 2100 expert software, based on computation of a 'RNA Integrity Number' (RIN) (25) . A RIN number is computed for each RNA profile (see Supplementary Table 4 online) resulting in the classification of RNA samples in 10 numerically predefined categories of integrity. The values of the mean fold changes, calculated according to the 2 ÀDDCt quantification method (see Materials and Methods), were found lower than 1.0, corresponding to the expression level (1·) in the sample exhibiting the highest RNA quality (Table 2 and Figure 5 ).	./cache/cord-048222-1pq6dkl5.txt	./txt/cord-048222-1pq6dkl5.txt