id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-146406-85usg3uh	Morelli, Marco J.	Beyond the consensus: dissecting within-host viral population diversity of foot-and-mouth disease virus using next-generation genome sequencing	2011-01-27		.txt	text/plain	4376	221	55	Using next-generation sequencing (NGS) performed on a Genome Analyzer platform (Illumina), this study compared the viral populations within two bovine epithelial samples (foot lesions) from a single animal with the Inoculum used to initiate experimental infection. Some of the higher frequency polymorphisms identified encoded changes within codons associated with heparan sulphate binding and were present in both feet lesions revealing intermediate stages in the evolution of a tissue-culture adapted virus replicating within a mammalian host. The sequence diversity of viral populations within individual hosts is the starting 24 material for selection and subsequent evolution of RNA viruses such as foot--and--mouth 25 disease virus (FMDV). The sequence diversity of viral populations within individual hosts is the starting 24 material for selection and subsequent evolution of RNA viruses such as foot--and--mouth 25 disease virus (FMDV). Validation and analysis of sequence diversity in the samples 148 The frequency of site--specific polymorphisms was estimated from the frequency of 149 mismatches of the aligned reads to the reference genome.	./cache/cord-146406-85usg3uh.txt	./txt/cord-146406-85usg3uh.txt