id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-253307-4bpdfgau	Sanz, Miguel A.	Inhibition of host protein synthesis by Sindbis virus: correlation with viral RNA replication and release of nuclear proteins to the cytoplasm	2014-11-19		.txt	text/plain	10157	537	49	Analysis of a SINV variant with a point mutation in nsP2, exhibiting defects in the shut‐off of host protein synthesis, indicates that both viral RNA replication and the release of nuclear proteins to the cytoplasm are greatly inhibited. Furthermore, nucleoside analogues that inhibit cellular and viral RNA synthesis impede the blockade of host mRNA translation, in addition to the release of nuclear proteins. Therefore, in SINV nsP2 (P726G)-replicating BHK cells, a correlation exists between the inhibition of viral RNA replication and the release of nuclear proteins, in addition to the failure to completely block cellular mRNA translation. Thus, in agreement with previous results, these findings indicate that the shutoff of host translation after SINV infection is restricted when viral RNA synthesis is blocked and nuclear proteins are not released to the cytoplasm. Indeed, in cells co-transfected with rep C+luc(ΔnsPs) and EMCV(IRES)-nsP1-4 mRNA, there is low-level RNA replication, no release of nuclear proteins and the synthesis of cellular proteins is not blocked.	./cache/cord-253307-4bpdfgau.txt	./txt/cord-253307-4bpdfgau.txt