id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-254210-3mi2aop5	Haddad, Rodrigo	Silencing of HTLV-1 gag and env genes by small interfering RNAs in HEK 293 cells	2011-01-26		.txt	text/plain	4490	281	57	title: Silencing of HTLV-1 gag and env genes by small interfering RNAs in HEK 293 cells Three small interference RNAs (siRNAs) corresponding to gag and three corresponding to env were designed to analyze the effect of silencing by RNAi technology. In the present study, siRNAs were used for inhibition of the expression of the HTLV-1 structural genes gag and env. Forty-eight hours after transfection, the expression of EGFP-target (gag or env) fusion proteins was observed directly under an inverted fluorescence microscope. HEK 293 cells were observed 48 h posttransfection under a fluorescence microscope to determine the silencing effect of siRNAs on Gag and Env protein expression in cultured cells (Fig. 2B and C) . Based on the fluorescence data, flow cytometry and real time quantitative PCR, two siRNAs targeted to the HTLV-1 gag gene reduced efficiently gene expression by different amounts compared to the negative siRNA, scrambled siRNAs and controls without siRNA.	./cache/cord-254210-3mi2aop5.txt	./txt/cord-254210-3mi2aop5.txt