id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-256561-fnh2do4z	Barik, Sailen	Therapy of Respiratory Viral Infections with Intranasal siRNAs	2014-09-23		.txt	text/plain	2605	181	60	Based on these results, we propose the following consensus for designing intranasal antiviral siRNAs: (a) modified 19–27 nt-long double-stranded siRNAs are functional in the lung, (b) excessive 2′-OMe and 2′-F modifications in either or both strands of these siRNAs reduce efficacy, (c) limited modifications in the sense strand are beneficial, although their precise efficacy may be position-dependent, (d) cocktail of multiple siRNAs can be highly effective against multiple viral strains and subtypes. Therefore, enhancement of the intracellular and extracellular stability of synthetic siRNAs while increasing (or without compromising) their RNAi activity is a continuing goal for therapeutic translation of RNAi. A variety of chemical modifi cations, including terminal and internal ones, have been added to the fi rst-generation siRNA sequences to improve stability and delivery, leading to what we call "second-generation" siRNAs. Advantage has been taken of the free 2′-OH group of the ribose moiety of RNA (in contrast to DNA that lacks this OH group), to which various substituents were added.	./cache/cord-256561-fnh2do4z.txt	./txt/cord-256561-fnh2do4z.txt