id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-262592-0rdiosxd	Cuevas, José M.	Human norovirus hyper-mutation revealed by ultra-deep sequencing	2016-04-17		.txt	text/plain	5828	276	54	This revealed the presence of low-frequency sequences carrying large numbers of U-to-C or A-to-G base transitions, suggesting a role for hyper-mutation in NoV diversity. Based on the sequence context of the observed changes, we propose that NoV hypermutation might be driven by ADAR-mediated editing of the viral genomic RNA of either polarity during replication. We used 16 stool samples from patients acutely infected with NoV GII.4 to amplify by RT-PCR a 386-base region encompassing nucleotides 1 to 386 of the VP1 gene (reference sequence: GenBank JX459908; Fig. 1A ). After 48 h incubation, total RNA was extracted from cells, residual DNA was removed with DNAse I, a specific primer annealing to the minus-strand of the VP1 capsid gene was used for reverse transcription, and high-fidelity PCR amplification of a region encompassing positions 19 to 323 of the VP1 gene (305 bases, although only the 266 bases excluding primer regions Fig. 1 .	./cache/cord-262592-0rdiosxd.txt	./txt/cord-262592-0rdiosxd.txt