id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-262609-cssgzvus	Sivakumaran, K	RNA sequence and secondary structural determinants in a minimal viral promoter that directs replicase recognition and initiation of genomic plus-strand RNA synthesis	1999-12-03		.txt	text/plain	10580	550	55	title: RNA sequence and secondary structural determinants in a minimal viral promoter that directs replicase recognition and initiation of genomic plus-strand RNA synthesis In contrast, we find that position-specific changes in the RNA sequence will affect replicase recognition, modulate the polymerization process, and contribute to the differential accumulation of viral RNAs. These functional results are in agreement with the phylogenetic analysis of BMV and related viral sequences and suggest that a similar mechanism of RNA synthesis takes place for members of the alphavirus superfamily. Results herein demonstrate that the endscript directing genomic plus-strand synthesis also interacts with the viral replicase via a mechanism that does not depend on a speci®c preformed RNA structure. An interesting conclusion from our analysis of the BMV genomic minus-strand RNA endscript is that template sequence may regulate the amount of RNA synthesis in a manner independent of replicase-promoter interaction.	./cache/cord-262609-cssgzvus.txt	./txt/cord-262609-cssgzvus.txt