id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-263033-4790dhc5	Laptev, I. G.	Posttranscriptional modification of messenger RNAs in eukaryotes	2015-12-11		.txt	text/plain	6792	437	58	The review considers posttranscriptional modification of eukaryotic mRNA, focusing on the major modified nucleotides, the role they play in the cell, the methods to detect them, and the enzymes responsible for modification. Regions distant from the mRNA ends may contain N6 methyladenosine (m 6 A), 5 methylcytidine (m 5 C), pseudouridine (Ψ), and inosine (I), which were believed to play only a minor role because their pro portion in cell RNA is extremely low as compared with the standard nucleotides. In the case of eukaryotic mRNAs, the method is suitable for probing the adenosine methylation status in a particular site of a particular RNA in various cell growth conditions. A method known as site specific cleavage and radioactive labeling followed by ligation assisted extrac tion and thin layer chromatography (SCARLET) [23] makes it possible to establish whether adenosine is methylated in a given position of a given molecule and to estimate the proportion of modified and unmodi fied nucleotides (Fig. 2) .	./cache/cord-263033-4790dhc5.txt	./txt/cord-263033-4790dhc5.txt